AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1310150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1310150 MtrunA17_Chr8g0337261 85.882 170 4 3 1 168 1 152 1.61e-55 172
Msa1310150 MtrunA17_Chr8g0337251 64.286 168 23 8 1 168 1 131 1.11e-31 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 77 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCCCTCAACTTTATTT+AGG 0.238868 8_4:+6678684 Msa1310150:three_prime_UTR
AAGTTTAAGCGTGGAAAATT+TGG 0.266461 8_4:+6678455 Msa1310150:CDS
CAACTTTATTTAGGTCTGTT+TGG 0.271608 8_4:+6678693 Msa1310150:three_prime_UTR
ATGAACAATTATTGTTCATT+TGG 0.293223 8_4:+6678616 Msa1310150:three_prime_UTR
AAGTATAAGCATGGAAAATT+TGG 0.307873 8_4:+6678398 Msa1310150:CDS
TATCATCATGGTTATGGTTA+TGG 0.313342 8_4:+6678368 Msa1310150:CDS
AAGCATGGTAGGTTTGGTTT+TGG 0.332336 8_4:+6678431 Msa1310150:CDS
TCTTATCATGCTCCACAATA+TGG 0.348923 8_4:+6677724 Msa1310150:CDS
TATGGCTATCCATATCCTTC+TGG 0.365272 8_4:+6677742 Msa1310150:CDS
GGAGGTTATCATCATGGTTA+TGG 0.376411 8_4:+6678362 Msa1310150:CDS
CGTTGGAAGCATGGTAGGTT+TGG 0.389621 8_4:+6678425 Msa1310150:CDS
TCAAGTGTACCAGAAGGATA+TGG 0.413672 8_4:-6677751 None:intergenic
CCTTCCGGTTATCCTCATTC+CGG 0.421172 8_4:+6677685 Msa1310150:CDS
GAACCAGGTGGAGGAGGATA+AGG 0.429948 8_4:-6677550 None:intergenic
GGAGGATAAGGTGGTGCTGC+TGG 0.432218 8_4:-6677538 None:intergenic
TGTTATAAAGGGCGTGGATC+TGG 0.441402 8_4:+6678275 Msa1310150:intron
ATATCCGGAATGAGGATAAC+CGG 0.442176 8_4:-6677689 None:intergenic
GGTATAGGAGGATTAATTGC+AGG 0.442489 8_4:+6678296 Msa1310150:CDS
GCAAGCGATGGAAGTGATGC+AGG 0.445817 8_4:+6678477 Msa1310150:exon
AGGTTATTATCCACCGCAAC+CGG 0.446409 8_4:+6677594 Msa1310150:CDS
GGAGGAGGGTATCCACCTTC+CGG 0.452585 8_4:+6677670 Msa1310150:CDS
GCAGGGGCGGCAGCTGCATA+CGG 0.453407 8_4:+6678314 Msa1310150:CDS
AAAGGGCGTGGATCTGGTAT+AGG 0.462773 8_4:+6678281 Msa1310150:intron
CACCATCTGTCGCATGGACA+TGG 0.469597 8_4:+6678341 Msa1310150:CDS
CATGGTTATGGTTATGGACA+TGG 0.469853 8_4:+6678374 Msa1310150:CDS
CCACCACATGCTGCAGGAGG+AGG 0.490677 8_4:+6677655 Msa1310150:CDS
AGATGGTTGATGATGATATC+CGG 0.500001 8_4:-6677704 None:intergenic
TTTGGCAAGCGTTGGAAGCA+TGG 0.515401 8_4:+6678416 Msa1310150:CDS
CCTCCTTGAGGTGGATATGC+CGG 0.520114 8_4:-6677613 None:intergenic
ACAGACCTAAATAAAGTTGA+GGG 0.520171 8_4:-6678689 None:intergenic
ATGGAAAATTTGGCAAGCGT+TGG 0.520922 8_4:+6678408 Msa1310150:CDS
CCACCTTATCCTCCTCCACC+TGG 0.523771 8_4:+6677547 Msa1310150:CDS
GAATAAGAACCAGGTGGAGG+AGG 0.530977 8_4:-6677556 None:intergenic
CTCCATGTCCATGCGACAGA+TGG 0.531469 8_4:-6678343 None:intergenic
ATAACCTCCTTGAGGTGGAT+AGG 0.535330 8_4:-6677578 None:intergenic
GCAAGCGTTGGAAGCATGGT+AGG 0.542067 8_4:+6678420 Msa1310150:CDS
AACAGACCTAAATAAAGTTG+AGG 0.548568 8_4:-6678690 None:intergenic
CAATATCCACCACATGCTGC+AGG 0.549438 8_4:+6677649 Msa1310150:CDS
CACCACATGCTGCAGGAGGA+GGG 0.555583 8_4:+6677656 Msa1310150:CDS
TGAGGTGGATATGCCGGTTG+CGG 0.565399 8_4:-6677607 None:intergenic
GGACATGGAGGTTATCATCA+TGG 0.565727 8_4:+6678356 Msa1310150:CDS
GGATATGGATAGCCATATTG+TGG 0.568164 8_4:-6677736 None:intergenic
CAAGCGATGGAAGTGATGCA+GGG 0.575898 8_4:+6678478 Msa1310150:exon
TACCCTCCTCCTGCAGCATG+TGG 0.578490 8_4:-6677658 None:intergenic
CCTCCTCCTGCAGCATGTGG+TGG 0.578595 8_4:-6677655 None:intergenic
TCTTATTCCTATCCACCTCA+AGG 0.583936 8_4:+6677571 Msa1310150:CDS
GTATAGGAGGATTAATTGCA+GGG 0.585893 8_4:+6678297 Msa1310150:CDS
GGGCGTGGATCTGGTATAGG+AGG 0.586096 8_4:+6678284 Msa1310150:intron
TATTGTGGAGCATGATAAGA+TGG 0.586561 8_4:-6677721 None:intergenic
ACATGTCATAAGATATCTCA+TGG 0.598626 8_4:-6678735 None:intergenic
GGTGGATATGCCGGTTGCGG+TGG 0.601680 8_4:-6677604 None:intergenic
GGATAATAACCTCCTTGAGG+TGG 0.602130 8_4:-6677622 None:intergenic
GTGGAAAATTTGGCAAGCGA+TGG 0.603805 8_4:+6678465 Msa1310150:CDS
CCAGGTGGAGGAGGATAAGG+TGG 0.606697 8_4:-6677547 None:intergenic
GGACATGGGAAGTATAAGCA+TGG 0.607097 8_4:+6678389 Msa1310150:CDS
GGTGCTCACCATCTGTCGCA+TGG 0.609238 8_4:+6678335 Msa1310150:CDS
TAGGAATAAGAACCAGGTGG+AGG 0.612421 8_4:-6677559 None:intergenic
GGATAATAACCTCCTTGAGG+TGG 0.612862 8_4:-6677583 None:intergenic
GATCAAGAATCTAGTGAGAG+AGG 0.615149 8_4:+6677490 Msa1310150:CDS
AGGAGGATTAATTGCAGGGG+CGG 0.617439 8_4:+6678301 Msa1310150:CDS
ATGGTTATGGTTATGGACAT+GGG 0.618834 8_4:+6678375 Msa1310150:CDS
GAATGAGGATAACCGGAAGG+TGG 0.623841 8_4:-6677682 None:intergenic
GGTGGATAGGAATAAGAACC+AGG 0.628633 8_4:-6677565 None:intergenic
AAGCGATGGAAGTGATGCAG+GGG 0.637098 8_4:+6678479 Msa1310150:exon
TGATGATGATATCCGGAATG+AGG 0.639527 8_4:-6677697 None:intergenic
CAACCGGCATATCCACCTCA+AGG 0.642247 8_4:+6677610 Msa1310150:CDS
GGGTGAAACAACATGAACAA+TGG 0.660870 8_4:+6677454 None:intergenic
TATCCACCACATGCTGCAGG+AGG 0.666317 8_4:+6677652 Msa1310150:CDS
CCGGAATGAGGATAACCGGA+AGG 0.667322 8_4:-6677685 None:intergenic
GCATGTGGTGGATATTGTGA+AGG 0.678658 8_4:-6677643 None:intergenic
CATCTGTCGCATGGACATGG+AGG 0.679502 8_4:+6678344 Msa1310150:CDS
GAAGGATAATAACCTCCTTG+AGG 0.682876 8_4:-6677625 None:intergenic
TATTCCTATCCACCTCAAGG+AGG 0.698574 8_4:+6677574 Msa1310150:CDS
GGTGGATAATAACCTCCTTG+AGG 0.702028 8_4:-6677586 None:intergenic
GGATAGGAATAAGAACCAGG+TGG 0.722057 8_4:-6677562 None:intergenic
CCGGCATATCCACCTCAAGG+AGG 0.736973 8_4:+6677613 Msa1310150:CDS
TATAGGAGGATTAATTGCAG+GGG 0.742815 8_4:+6678298 Msa1310150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTATTGTTGTTATAAA+GGG + chr8_4:6678264-6678283 Msa1310150:intron 10.0%
!!! TAATTTATTGTTGTTATAAA+GGG + chr8_4:6678264-6678283 Msa1310150:intron 10.0%
!! AATATACTCCATAAAAAATT+GGG - chr8_4:6677781-6677800 None:intergenic 15.0%
!! TAATATACTCCATAAAAAAT+TGG - chr8_4:6677782-6677801 None:intergenic 15.0%
!!! TACTAGTTTCAAATATTATT+AGG + chr8_4:6677828-6677847 Msa1310150:intron 15.0%
!!! ATTTTCTGCTTTTTTTTATT+GGG + chr8_4:6677871-6677890 Msa1310150:intron 15.0%
!!! CTAATTTATTGTTGTTATAA+AGG + chr8_4:6678263-6678282 Msa1310150:intron 15.0%
!! AATATACTCCATAAAAAATT+GGG - chr8_4:6677781-6677800 None:intergenic 15.0%
!! TAATATACTCCATAAAAAAT+TGG - chr8_4:6677782-6677801 None:intergenic 15.0%
!!! TACTAGTTTCAAATATTATT+AGG + chr8_4:6677828-6677847 Msa1310150:intron 15.0%
!!! ATTTTCTGCTTTTTTTTATT+GGG + chr8_4:6677871-6677890 Msa1310150:intron 15.0%
!!! CTAATTTATTGTTGTTATAA+AGG + chr8_4:6678263-6678282 Msa1310150:intron 15.0%
!!! CATTTTCTGCTTTTTTTTAT+TGG + chr8_4:6677870-6677889 Msa1310150:intron 20.0%
!! ATGAACAATTATTGTTCATT+TGG + chr8_4:6678616-6678635 Msa1310150:three_prime_UTR 20.0%
!!! CATTTTCTGCTTTTTTTTAT+TGG + chr8_4:6677870-6677889 Msa1310150:intron 20.0%
!! ATGAACAATTATTGTTCATT+TGG + chr8_4:6678616-6678635 Msa1310150:three_prime_UTR 20.0%
!!! TTGATCATCCCAATTTTTTA+TGG + chr8_4:6677770-6677789 Msa1310150:intron 25.0%
!!! TATAAAGTGTTGTCAAATAG+AGG + chr8_4:6677965-6677984 Msa1310150:intron 25.0%
! AACAACATTGATCACAAAAA+TGG + chr8_4:6678057-6678076 Msa1310150:intron 25.0%
!!! AGATCAATGTTTTTTGCTAA+TGG + chr8_4:6678099-6678118 Msa1310150:intron 25.0%
! TAAAACAAAGACGTAATATC+AGG - chr8_4:6678178-6678197 None:intergenic 25.0%
! AAGTATAAGCATGGAAAATT+TGG + chr8_4:6678398-6678417 Msa1310150:CDS 25.0%
!!! TTGATCATCCCAATTTTTTA+TGG + chr8_4:6677770-6677789 Msa1310150:intron 25.0%
!!! TATAAAGTGTTGTCAAATAG+AGG + chr8_4:6677965-6677984 Msa1310150:intron 25.0%
! AACAACATTGATCACAAAAA+TGG + chr8_4:6678057-6678076 Msa1310150:intron 25.0%
!!! AGATCAATGTTTTTTGCTAA+TGG + chr8_4:6678099-6678118 Msa1310150:intron 25.0%
! TAAAACAAAGACGTAATATC+AGG - chr8_4:6678178-6678197 None:intergenic 25.0%
! AAGTATAAGCATGGAAAATT+TGG + chr8_4:6678398-6678417 Msa1310150:CDS 25.0%
CATCTATTACAGAACAATAG+CGG - chr8_4:6677939-6677958 None:intergenic 30.0%
CAGAATTTGTACAAACTATG+TGG + chr8_4:6678020-6678039 Msa1310150:intron 30.0%
TAACACATGCAAGAAACATT+AGG - chr8_4:6678135-6678154 None:intergenic 30.0%
TATCATCATGGTTATGGTTA+TGG + chr8_4:6678368-6678387 Msa1310150:CDS 30.0%
AAGTTTAAGCGTGGAAAATT+TGG + chr8_4:6678455-6678474 Msa1310150:CDS 30.0%
!!! AGCAAGTTTTGAATTTCAGT+TGG + chr8_4:6678584-6678603 Msa1310150:three_prime_UTR 30.0%
ACAGACCTAAATAAAGTTGA+GGG - chr8_4:6678692-6678711 None:intergenic 30.0%
AACAGACCTAAATAAAGTTG+AGG - chr8_4:6678693-6678712 None:intergenic 30.0%
! CAACTTTATTTAGGTCTGTT+TGG + chr8_4:6678693-6678712 Msa1310150:three_prime_UTR 30.0%
CATCTATTACAGAACAATAG+CGG - chr8_4:6677939-6677958 None:intergenic 30.0%
CAGAATTTGTACAAACTATG+TGG + chr8_4:6678020-6678039 Msa1310150:intron 30.0%
TAACACATGCAAGAAACATT+AGG - chr8_4:6678135-6678154 None:intergenic 30.0%
TATCATCATGGTTATGGTTA+TGG + chr8_4:6678368-6678387 Msa1310150:CDS 30.0%
AAGTTTAAGCGTGGAAAATT+TGG + chr8_4:6678455-6678474 Msa1310150:CDS 30.0%
!!! AGCAAGTTTTGAATTTCAGT+TGG + chr8_4:6678584-6678603 Msa1310150:three_prime_UTR 30.0%
ACAGACCTAAATAAAGTTGA+GGG - chr8_4:6678692-6678711 None:intergenic 30.0%
AACAGACCTAAATAAAGTTG+AGG - chr8_4:6678693-6678712 None:intergenic 30.0%
! CAACTTTATTTAGGTCTGTT+TGG + chr8_4:6678693-6678712 Msa1310150:three_prime_UTR 30.0%
! GGTCTGTTTTCACATATAAC+AGG + chr8_4:6677511-6677530 Msa1310150:CDS 35.0%
!! AGATGGTTGATGATGATATC+CGG - chr8_4:6677707-6677726 None:intergenic 35.0%
TATTGTGGAGCATGATAAGA+TGG - chr8_4:6677724-6677743 None:intergenic 35.0%
TCTTATCATGCTCCACAATA+TGG + chr8_4:6677724-6677743 Msa1310150:CDS 35.0%
CAAAGACGTAATATCAGGTA+TGG - chr8_4:6678173-6678192 None:intergenic 35.0%
! TATTGTTGTTATAAAGGGCG+TGG + chr8_4:6678269-6678288 Msa1310150:intron 35.0%
GTATAGGAGGATTAATTGCA+GGG + chr8_4:6678297-6678316 Msa1310150:CDS 35.0%
TATAGGAGGATTAATTGCAG+GGG + chr8_4:6678298-6678317 Msa1310150:CDS 35.0%
ATGGTTATGGTTATGGACAT+GGG + chr8_4:6678375-6678394 Msa1310150:CDS 35.0%
! GTCTTTTGTTCAAATGTGTG+TGG + chr8_4:6678646-6678665 Msa1310150:three_prime_UTR 35.0%
! GAAAGCCCTCAACTTTATTT+AGG + chr8_4:6678684-6678703 Msa1310150:three_prime_UTR 35.0%
! GGTCTGTTTTCACATATAAC+AGG + chr8_4:6677511-6677530 Msa1310150:CDS 35.0%
!! AGATGGTTGATGATGATATC+CGG - chr8_4:6677707-6677726 None:intergenic 35.0%
TATTGTGGAGCATGATAAGA+TGG - chr8_4:6677724-6677743 None:intergenic 35.0%
TCTTATCATGCTCCACAATA+TGG + chr8_4:6677724-6677743 Msa1310150:CDS 35.0%
CAAAGACGTAATATCAGGTA+TGG - chr8_4:6678173-6678192 None:intergenic 35.0%
! TATTGTTGTTATAAAGGGCG+TGG + chr8_4:6678269-6678288 Msa1310150:intron 35.0%
GTATAGGAGGATTAATTGCA+GGG + chr8_4:6678297-6678316 Msa1310150:CDS 35.0%
TATAGGAGGATTAATTGCAG+GGG + chr8_4:6678298-6678317 Msa1310150:CDS 35.0%
ATGGTTATGGTTATGGACAT+GGG + chr8_4:6678375-6678394 Msa1310150:CDS 35.0%
! GTCTTTTGTTCAAATGTGTG+TGG + chr8_4:6678646-6678665 Msa1310150:three_prime_UTR 35.0%
! GAAAGCCCTCAACTTTATTT+AGG + chr8_4:6678684-6678703 Msa1310150:three_prime_UTR 35.0%
GATCAAGAATCTAGTGAGAG+AGG + chr8_4:6677490-6677509 Msa1310150:CDS 40.0%
TCTTATTCCTATCCACCTCA+AGG + chr8_4:6677571-6677590 Msa1310150:CDS 40.0%
GAAGGATAATAACCTCCTTG+AGG - chr8_4:6677628-6677647 None:intergenic 40.0%
ATATCCGGAATGAGGATAAC+CGG - chr8_4:6677692-6677711 None:intergenic 40.0%
! TGATGATGATATCCGGAATG+AGG - chr8_4:6677700-6677719 None:intergenic 40.0%
GGATATGGATAGCCATATTG+TGG - chr8_4:6677739-6677758 None:intergenic 40.0%
TATGGCTATCCATATCCTTC+TGG + chr8_4:6677742-6677761 Msa1310150:CDS 40.0%
TCAAGTGTACCAGAAGGATA+TGG - chr8_4:6677754-6677773 None:intergenic 40.0%
!! CGGCTTGTTAAAATTCTGCT+AGG - chr8_4:6677919-6677938 None:intergenic 40.0%
GGTATAGGAGGATTAATTGC+AGG + chr8_4:6678296-6678315 Msa1310150:CDS 40.0%
GGAGGTTATCATCATGGTTA+TGG + chr8_4:6678362-6678381 Msa1310150:CDS 40.0%
CATGGTTATGGTTATGGACA+TGG + chr8_4:6678374-6678393 Msa1310150:CDS 40.0%
ATGGAAAATTTGGCAAGCGT+TGG + chr8_4:6678408-6678427 Msa1310150:CDS 40.0%
!! AAGCATGGTAGGTTTGGTTT+TGG + chr8_4:6678431-6678450 Msa1310150:CDS 40.0%
!!! GGTTTTGGAAAGTTTAAGCG+TGG + chr8_4:6678446-6678465 Msa1310150:CDS 40.0%
GATCAAGAATCTAGTGAGAG+AGG + chr8_4:6677490-6677509 Msa1310150:CDS 40.0%
TCTTATTCCTATCCACCTCA+AGG + chr8_4:6677571-6677590 Msa1310150:CDS 40.0%
GAAGGATAATAACCTCCTTG+AGG - chr8_4:6677628-6677647 None:intergenic 40.0%
ATATCCGGAATGAGGATAAC+CGG - chr8_4:6677692-6677711 None:intergenic 40.0%
! TGATGATGATATCCGGAATG+AGG - chr8_4:6677700-6677719 None:intergenic 40.0%
GGATATGGATAGCCATATTG+TGG - chr8_4:6677739-6677758 None:intergenic 40.0%
TATGGCTATCCATATCCTTC+TGG + chr8_4:6677742-6677761 Msa1310150:CDS 40.0%
TCAAGTGTACCAGAAGGATA+TGG - chr8_4:6677754-6677773 None:intergenic 40.0%
!! CGGCTTGTTAAAATTCTGCT+AGG - chr8_4:6677919-6677938 None:intergenic 40.0%
GGTATAGGAGGATTAATTGC+AGG + chr8_4:6678296-6678315 Msa1310150:CDS 40.0%
GGAGGTTATCATCATGGTTA+TGG + chr8_4:6678362-6678381 Msa1310150:CDS 40.0%
CATGGTTATGGTTATGGACA+TGG + chr8_4:6678374-6678393 Msa1310150:CDS 40.0%
ATGGAAAATTTGGCAAGCGT+TGG + chr8_4:6678408-6678427 Msa1310150:CDS 40.0%
!! AAGCATGGTAGGTTTGGTTT+TGG + chr8_4:6678431-6678450 Msa1310150:CDS 40.0%
!!! GGTTTTGGAAAGTTTAAGCG+TGG + chr8_4:6678446-6678465 Msa1310150:CDS 40.0%
TAGGAATAAGAACCAGGTGG+AGG - chr8_4:6677562-6677581 None:intergenic 45.0%
GGATAGGAATAAGAACCAGG+TGG - chr8_4:6677565-6677584 None:intergenic 45.0%
GGTGGATAGGAATAAGAACC+AGG - chr8_4:6677568-6677587 None:intergenic 45.0%
TATTCCTATCCACCTCAAGG+AGG + chr8_4:6677574-6677593 Msa1310150:CDS 45.0%
ATAACCTCCTTGAGGTGGAT+AGG - chr8_4:6677581-6677600 None:intergenic 45.0%
GGATAATAACCTCCTTGAGG+TGG - chr8_4:6677586-6677605 None:intergenic 45.0%
GGTGGATAATAACCTCCTTG+AGG - chr8_4:6677589-6677608 None:intergenic 45.0%
AGGTTATTATCCACCGCAAC+CGG + chr8_4:6677594-6677613 Msa1310150:CDS 45.0%
GGATAATAACCTCCTTGAGG+TGG - chr8_4:6677586-6677605 None:intergenic 45.0%
!! GCATGTGGTGGATATTGTGA+AGG - chr8_4:6677646-6677665 None:intergenic 45.0%
GGATGATCAAGTGTACCAGA+AGG - chr8_4:6677760-6677779 None:intergenic 45.0%
CAGAATGATGCTCGTTTGAG+AGG + chr8_4:6678221-6678240 Msa1310150:intron 45.0%
! TGTTATAAAGGGCGTGGATC+TGG + chr8_4:6678275-6678294 Msa1310150:intron 45.0%
GGACATGGAGGTTATCATCA+TGG + chr8_4:6678356-6678375 Msa1310150:CDS 45.0%
! GGACATGGGAAGTATAAGCA+TGG + chr8_4:6678389-6678408 Msa1310150:CDS 45.0%
GTGGAAAATTTGGCAAGCGA+TGG + chr8_4:6678465-6678484 Msa1310150:CDS 45.0%
TAGGAATAAGAACCAGGTGG+AGG - chr8_4:6677562-6677581 None:intergenic 45.0%
GGATAGGAATAAGAACCAGG+TGG - chr8_4:6677565-6677584 None:intergenic 45.0%
GGTGGATAGGAATAAGAACC+AGG - chr8_4:6677568-6677587 None:intergenic 45.0%
TATTCCTATCCACCTCAAGG+AGG + chr8_4:6677574-6677593 Msa1310150:CDS 45.0%
ATAACCTCCTTGAGGTGGAT+AGG - chr8_4:6677581-6677600 None:intergenic 45.0%
GGATAATAACCTCCTTGAGG+TGG - chr8_4:6677586-6677605 None:intergenic 45.0%
GGTGGATAATAACCTCCTTG+AGG - chr8_4:6677589-6677608 None:intergenic 45.0%
AGGTTATTATCCACCGCAAC+CGG + chr8_4:6677594-6677613 Msa1310150:CDS 45.0%
GGATAATAACCTCCTTGAGG+TGG - chr8_4:6677586-6677605 None:intergenic 45.0%
!! GCATGTGGTGGATATTGTGA+AGG - chr8_4:6677646-6677665 None:intergenic 45.0%
GGATGATCAAGTGTACCAGA+AGG - chr8_4:6677760-6677779 None:intergenic 45.0%
CAGAATGATGCTCGTTTGAG+AGG + chr8_4:6678221-6678240 Msa1310150:intron 45.0%
! TGTTATAAAGGGCGTGGATC+TGG + chr8_4:6678275-6678294 Msa1310150:intron 45.0%
GGACATGGAGGTTATCATCA+TGG + chr8_4:6678356-6678375 Msa1310150:CDS 45.0%
! GGACATGGGAAGTATAAGCA+TGG + chr8_4:6678389-6678408 Msa1310150:CDS 45.0%
GTGGAAAATTTGGCAAGCGA+TGG + chr8_4:6678465-6678484 Msa1310150:CDS 45.0%
GAATAAGAACCAGGTGGAGG+AGG - chr8_4:6677559-6677578 None:intergenic 50.0%
CAATATCCACCACATGCTGC+AGG + chr8_4:6677649-6677668 Msa1310150:CDS 50.0%
GAATGAGGATAACCGGAAGG+TGG - chr8_4:6677685-6677704 None:intergenic 50.0%
CCTTCCGGTTATCCTCATTC+CGG + chr8_4:6677685-6677704 Msa1310150:CDS 50.0%
! AAAGGGCGTGGATCTGGTAT+AGG + chr8_4:6678281-6678300 Msa1310150:intron 50.0%
AGGAGGATTAATTGCAGGGG+CGG + chr8_4:6678301-6678320 Msa1310150:CDS 50.0%
!! TTTGGCAAGCGTTGGAAGCA+TGG + chr8_4:6678416-6678435 Msa1310150:CDS 50.0%
! CGTTGGAAGCATGGTAGGTT+TGG + chr8_4:6678425-6678444 Msa1310150:CDS 50.0%
CAAGCGATGGAAGTGATGCA+GGG + chr8_4:6678478-6678497 Msa1310150:exon 50.0%
AAGCGATGGAAGTGATGCAG+GGG + chr8_4:6678479-6678498 Msa1310150:exon 50.0%
GAATAAGAACCAGGTGGAGG+AGG - chr8_4:6677559-6677578 None:intergenic 50.0%
CAATATCCACCACATGCTGC+AGG + chr8_4:6677649-6677668 Msa1310150:CDS 50.0%
GAATGAGGATAACCGGAAGG+TGG - chr8_4:6677685-6677704 None:intergenic 50.0%
CCTTCCGGTTATCCTCATTC+CGG + chr8_4:6677685-6677704 Msa1310150:CDS 50.0%
! AAAGGGCGTGGATCTGGTAT+AGG + chr8_4:6678281-6678300 Msa1310150:intron 50.0%
AGGAGGATTAATTGCAGGGG+CGG + chr8_4:6678301-6678320 Msa1310150:CDS 50.0%
!! TTTGGCAAGCGTTGGAAGCA+TGG + chr8_4:6678416-6678435 Msa1310150:CDS 50.0%
! CGTTGGAAGCATGGTAGGTT+TGG + chr8_4:6678425-6678444 Msa1310150:CDS 50.0%
CAAGCGATGGAAGTGATGCA+GGG + chr8_4:6678478-6678497 Msa1310150:exon 50.0%
AAGCGATGGAAGTGATGCAG+GGG + chr8_4:6678479-6678498 Msa1310150:exon 50.0%
GAACCAGGTGGAGGAGGATA+AGG - chr8_4:6677553-6677572 None:intergenic 55.0%
! TGAGGTGGATATGCCGGTTG+CGG - chr8_4:6677610-6677629 None:intergenic 55.0%
CAACCGGCATATCCACCTCA+AGG + chr8_4:6677610-6677629 Msa1310150:CDS 55.0%
CCTCCTTGAGGTGGATATGC+CGG - chr8_4:6677616-6677635 None:intergenic 55.0%
TATCCACCACATGCTGCAGG+AGG + chr8_4:6677652-6677671 Msa1310150:CDS 55.0%
CCGGAATGAGGATAACCGGA+AGG - chr8_4:6677688-6677707 None:intergenic 55.0%
CACCATCTGTCGCATGGACA+TGG + chr8_4:6678341-6678360 Msa1310150:CDS 55.0%
CTCCATGTCCATGCGACAGA+TGG - chr8_4:6678346-6678365 None:intergenic 55.0%
CATCTGTCGCATGGACATGG+AGG + chr8_4:6678344-6678363 Msa1310150:CDS 55.0%
!! GCAAGCGTTGGAAGCATGGT+AGG + chr8_4:6678420-6678439 Msa1310150:CDS 55.0%
GCAAGCGATGGAAGTGATGC+AGG + chr8_4:6678477-6678496 Msa1310150:exon 55.0%
GAACCAGGTGGAGGAGGATA+AGG - chr8_4:6677553-6677572 None:intergenic 55.0%
! TGAGGTGGATATGCCGGTTG+CGG - chr8_4:6677610-6677629 None:intergenic 55.0%
CAACCGGCATATCCACCTCA+AGG + chr8_4:6677610-6677629 Msa1310150:CDS 55.0%
CCTCCTTGAGGTGGATATGC+CGG - chr8_4:6677616-6677635 None:intergenic 55.0%
TATCCACCACATGCTGCAGG+AGG + chr8_4:6677652-6677671 Msa1310150:CDS 55.0%
CCGGAATGAGGATAACCGGA+AGG - chr8_4:6677688-6677707 None:intergenic 55.0%
CACCATCTGTCGCATGGACA+TGG + chr8_4:6678341-6678360 Msa1310150:CDS 55.0%
CTCCATGTCCATGCGACAGA+TGG - chr8_4:6678346-6678365 None:intergenic 55.0%
CATCTGTCGCATGGACATGG+AGG + chr8_4:6678344-6678363 Msa1310150:CDS 55.0%
!! GCAAGCGTTGGAAGCATGGT+AGG + chr8_4:6678420-6678439 Msa1310150:CDS 55.0%
GCAAGCGATGGAAGTGATGC+AGG + chr8_4:6678477-6678496 Msa1310150:exon 55.0%
!! GGAGGATAAGGTGGTGCTGC+TGG - chr8_4:6677541-6677560 None:intergenic 60.0%
CCAGGTGGAGGAGGATAAGG+TGG - chr8_4:6677550-6677569 None:intergenic 60.0%
CCACCTTATCCTCCTCCACC+TGG + chr8_4:6677547-6677566 Msa1310150:CDS 60.0%
CCGGCATATCCACCTCAAGG+AGG + chr8_4:6677613-6677632 Msa1310150:CDS 60.0%
CACCACATGCTGCAGGAGGA+GGG + chr8_4:6677656-6677675 Msa1310150:CDS 60.0%
TACCCTCCTCCTGCAGCATG+TGG - chr8_4:6677661-6677680 None:intergenic 60.0%
!! GGAGGAGGGTATCCACCTTC+CGG + chr8_4:6677670-6677689 Msa1310150:CDS 60.0%
GGGCGTGGATCTGGTATAGG+AGG + chr8_4:6678284-6678303 Msa1310150:intron 60.0%
GGTGCTCACCATCTGTCGCA+TGG + chr8_4:6678335-6678354 Msa1310150:CDS 60.0%
!! GGAGGATAAGGTGGTGCTGC+TGG - chr8_4:6677541-6677560 None:intergenic 60.0%
CCAGGTGGAGGAGGATAAGG+TGG - chr8_4:6677550-6677569 None:intergenic 60.0%
CCACCTTATCCTCCTCCACC+TGG + chr8_4:6677547-6677566 Msa1310150:CDS 60.0%
CCGGCATATCCACCTCAAGG+AGG + chr8_4:6677613-6677632 Msa1310150:CDS 60.0%
CACCACATGCTGCAGGAGGA+GGG + chr8_4:6677656-6677675 Msa1310150:CDS 60.0%
TACCCTCCTCCTGCAGCATG+TGG - chr8_4:6677661-6677680 None:intergenic 60.0%
!! GGAGGAGGGTATCCACCTTC+CGG + chr8_4:6677670-6677689 Msa1310150:CDS 60.0%
GGGCGTGGATCTGGTATAGG+AGG + chr8_4:6678284-6678303 Msa1310150:intron 60.0%
GGTGCTCACCATCTGTCGCA+TGG + chr8_4:6678335-6678354 Msa1310150:CDS 60.0%
! GGTGGATATGCCGGTTGCGG+TGG - chr8_4:6677607-6677626 None:intergenic 65.0%
CCTCCTCCTGCAGCATGTGG+TGG - chr8_4:6677658-6677677 None:intergenic 65.0%
CCACCACATGCTGCAGGAGG+AGG + chr8_4:6677655-6677674 Msa1310150:CDS 65.0%
! GGTGGATATGCCGGTTGCGG+TGG - chr8_4:6677607-6677626 None:intergenic 65.0%
CCTCCTCCTGCAGCATGTGG+TGG - chr8_4:6677658-6677677 None:intergenic 65.0%
CCACCACATGCTGCAGGAGG+AGG + chr8_4:6677655-6677674 Msa1310150:CDS 65.0%
GCAGGGGCGGCAGCTGCATA+CGG + chr8_4:6678314-6678333 Msa1310150:CDS 70.0%
GCAGGGGCGGCAGCTGCATA+CGG + chr8_4:6678314-6678333 Msa1310150:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 6677466 6678748 6677466 ID=Msa1310150;Name=Msa1310150
chr8_4 mRNA 6677466 6678748 6677466 ID=Msa1310150-mRNA-1;Parent=Msa1310150;Name=Msa1310150-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|1|0.5|1|1|1|2|255|168
chr8_4 exon 6677466 6677763 6677466 ID=Msa1310150-mRNA-1:exon:2244;Parent=Msa1310150-mRNA-1
chr8_4 exon 6678285 6678748 6678285 ID=Msa1310150-mRNA-1:exon:2245;Parent=Msa1310150-mRNA-1
chr8_4 CDS 6677466 6677763 6677466 ID=Msa1310150-mRNA-1:cds;Parent=Msa1310150-mRNA-1
chr8_4 CDS 6678285 6678493 6678285 ID=Msa1310150-mRNA-1:cds;Parent=Msa1310150-mRNA-1
chr8_4 three_prime_UTR 6678494 6678748 6678494 ID=Msa1310150-mRNA-1:three_prime_utr;Parent=Msa1310150-mRNA-1
Gene Sequence

>Msa1310150

ATGAACAATGGTAGAAACAAGCATGATCAAGAATCTAGTGAGAGAGGTCTGTTTTCACATATAACAGGCTATCCAGCAGCACCACCTTATCCTCCTCCACCTGGTTCTTATTCCTATCCACCTCAAGGAGGTTATTATCCACCGCAACCGGCATATCCACCTCAAGGAGGTTATTATCCTTCACAATATCCACCACATGCTGCAGGAGGAGGGTATCCACCTTCCGGTTATCCTCATTCCGGATATCATCATCAACCATCTTATCATGCTCCACAATATGGCTATCCATATCCTTCTGGGCGTGGATCTGGTATAGGAGGATTAATTGCAGGGGCGGCAGCTGCATACGGTGCTCACCATCTGTCGCATGGACATGGAGGTTATCATCATGGTTATGGTTATGGACATGGGAAGTATAAGCATGGAAAATTTGGCAAGCGTTGGAAGCATGGTAGGTTTGGTTTTGGAAAGTTTAAGCGTGGAAAATTTGGCAAGCGATGGAAGTGA
Protein sequence

>Msa1310150

MNNGRNKHDQESSERGLFSHITGYPAAPPYPPPPGSYSYPPQGGYYPPQPAYPPQGGYYPSQYPPHAAGGGYPPSGYPHSGYHHQPSYHAPQYGYPYPSGRGSGIGGLIAGAAAAYGAHHLSHGHGGYHHGYGYGHGKYKHGKFGKRWKHGRFGFGKFKRGKFGKRWK*