Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1233010 | XP_003627099.1 | 98.515 | 202 | 2 | 1 | 1 | 201 | 1 | 202 | 1.28e-141 | 403 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1233010 | G7LHF9 | 98.515 | 202 | 2 | 1 | 1 | 201 | 1 | 202 | 6.09e-142 | 403 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1233010 | Msa1275450 | 0.800869 | 1.177126e-48 | -8.615850e-47 |
| Msa0427700 | Msa1233010 | 0.802060 | 6.709289e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1233010 | MtrunA17_Chr8g0342361 | 98.515 | 202 | 2 | 1 | 1 | 201 | 1 | 202 | 1.17e-145 | 403 |
| Msa1233010 | MtrunA17_Chr1g0202491 | 43.000 | 200 | 87 | 6 | 1 | 181 | 1 | 192 | 2.68e-42 | 141 |
| Msa1233010 | MtrunA17_Chr6g0469901 | 35.827 | 254 | 98 | 8 | 1 | 196 | 1 | 247 | 4.52e-35 | 124 |
| Msa1233010 | MtrunA17_Chr8g0388471 | 31.148 | 183 | 89 | 8 | 1 | 160 | 1 | 169 | 2.09e-14 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1233010 | AT3G61920.1 | 45.960 | 198 | 81 | 6 | 1 | 186 | 1 | 184 | 9.23e-42 | 139 |
| Msa1233010 | AT1G64700.1 | 36.559 | 186 | 90 | 7 | 1 | 161 | 1 | 183 | 2.59e-28 | 105 |
Find 40 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATATTGTTAAACAATT+TGG | 0.119340 | 8_2:+9646159 | Msa1233010:CDS |
| CAAATTGTTTAACAATATTT+TGG | 0.127794 | 8_2:-9646158 | None:intergenic |
| ACAATAACAATAACAATATT+TGG | 0.279085 | 8_2:+9646241 | Msa1233010:CDS |
| GGTTATGCATTCAACCGTTA+TGG | 0.318183 | 8_2:-9646025 | None:intergenic |
| CGGTGGCTAAGTGCGGTAAC+GGG | 0.346940 | 8_2:+9646397 | Msa1233010:CDS |
| TGGCGTGTGGAAGGTGAAAT+TGG | 0.388861 | 8_2:+9646299 | Msa1233010:CDS |
| GTAAGAATTGTAACATCAAA+TGG | 0.436383 | 8_2:+9645979 | Msa1233010:CDS |
| CGGGGTGCCTTCATCATCTT+CGG | 0.443512 | 8_2:+9646416 | Msa1233010:CDS |
| AAAATCTCCGACAACTTCTC+CGG | 0.445716 | 8_2:-9646330 | None:intergenic |
| ACGGTGGCTAAGTGCGGTAA+CGG | 0.449663 | 8_2:+9646396 | Msa1233010:CDS |
| GTTATGCATTCAACCGTTAT+GGG | 0.458911 | 8_2:-9646024 | None:intergenic |
| AACATCAAATGGAGGCATCA+TGG | 0.506675 | 8_2:+9645990 | Msa1233010:CDS |
| CGGTGGCAAACTCCGATCAA+TGG | 0.517287 | 8_2:+9646436 | Msa1233010:CDS |
| GGAACTTTATTCTCCCATAA+CGG | 0.519483 | 8_2:+9646011 | Msa1233010:CDS |
| CAATAGTAGTGGCGTGTGGA+AGG | 0.523746 | 8_2:+9646290 | Msa1233010:CDS |
| GGTGAAATTGGTGATAAGTC+CGG | 0.528772 | 8_2:+9646311 | Msa1233010:CDS |
| ATGGGTAATTGCGTATTCAA+AGG | 0.536080 | 8_2:+9645925 | Msa1233010:CDS |
| GTATTCAAAGGCTTTCATCA+TGG | 0.561060 | 8_2:+9645937 | Msa1233010:CDS |
| ACCAACGAGTTCCCACATCA+TGG | 0.565815 | 8_2:+9646045 | Msa1233010:CDS |
| GTTGCTATTCTTGTCACATG+TGG | 0.579217 | 8_2:-9646217 | None:intergenic |
| GATAAGTCCGGAGAAGTTGT+CGG | 0.582804 | 8_2:+9646323 | Msa1233010:CDS |
| TAACAATAACAATATTTGGT+CGG | 0.587255 | 8_2:+9646245 | Msa1233010:CDS |
| GGTGGCTAAGTGCGGTAACG+GGG | 0.589494 | 8_2:+9646398 | Msa1233010:CDS |
| TTTGCCACCGAAGATGATGA+AGG | 0.601663 | 8_2:-9646423 | None:intergenic |
| TCACATGTGGACATTCGATA+CGG | 0.603282 | 8_2:-9646204 | None:intergenic |
| ACTTGACACACTCCATTGAT+CGG | 0.607173 | 8_2:-9646448 | None:intergenic |
| GTGGTTTAGAGAGTGTGTTA+CGG | 0.621760 | 8_2:-9646083 | None:intergenic |
| GTTGATAGAGAGTGTGAGGA+CGG | 0.635016 | 8_2:+9646377 | Msa1233010:CDS |
| TTTCCGAGGTACAATAGTAG+TGG | 0.636988 | 8_2:+9646279 | Msa1233010:CDS |
| GGTGCCTTCATCATCTTCGG+TGG | 0.641775 | 8_2:+9646419 | Msa1233010:CDS |
| TCCATGATGTGGGAACTCGT+TGG | 0.650105 | 8_2:-9646046 | None:intergenic |
| AGAATTGTAACATCAAATGG+AGG | 0.652719 | 8_2:+9645982 | Msa1233010:CDS |
| GTCGCAAGAGTCGAGGACGG+AGG | 0.655770 | 8_2:+9646353 | Msa1233010:CDS |
| AGGCGTTGATAGAGAGTGTG+AGG | 0.660586 | 8_2:+9646373 | Msa1233010:CDS |
| GTGAGGACGGTGGCTAAGTG+CGG | 0.661155 | 8_2:+9646390 | Msa1233010:CDS |
| TTTGTCGCAAGAGTCGAGGA+CGG | 0.662433 | 8_2:+9646350 | Msa1233010:CDS |
| CGGAACATGAAGTGTTTCCG+AGG | 0.663787 | 8_2:+9646265 | Msa1233010:CDS |
| GGTACAATAGTAGTGGCGTG+TGG | 0.679504 | 8_2:+9646286 | Msa1233010:CDS |
| ACGCCACTACTATTGTACCT+CGG | 0.689786 | 8_2:-9646282 | None:intergenic |
| GATAGAGAGTGTGAGGACGG+TGG | 0.695119 | 8_2:+9646380 | Msa1233010:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAAATTGTTTAACAATATTT+TGG | - | chr8_2:9646161-9646180 | None:intergenic | 15.0% |
| !! | CAAAATATTGTTAAACAATT+TGG | + | chr8_2:9646159-9646178 | Msa1233010:CDS | 15.0% |
| !! | ACAATAACAATAACAATATT+TGG | + | chr8_2:9646241-9646260 | Msa1233010:CDS | 15.0% |
| !! | TAACAATAACAATATTTGGT+CGG | + | chr8_2:9646245-9646264 | Msa1233010:CDS | 20.0% |
| ! | GTAAGAATTGTAACATCAAA+TGG | + | chr8_2:9645979-9645998 | Msa1233010:CDS | 25.0% |
| !!! | TTTTGAAAATTCCATGATGT+GGG | - | chr8_2:9646059-9646078 | None:intergenic | 25.0% |
| !!! | TTTTTGAAAATTCCATGATG+TGG | - | chr8_2:9646060-9646079 | None:intergenic | 25.0% |
| !!! | GTTTAACAATATTTTGGAGA+GGG | - | chr8_2:9646155-9646174 | None:intergenic | 25.0% |
| !!! | TGTTTAACAATATTTTGGAG+AGG | - | chr8_2:9646156-9646175 | None:intergenic | 25.0% |
| !! | TGGAGATTTTGAAGAAACAA+TGG | + | chr8_2:9645957-9645976 | Msa1233010:CDS | 30.0% |
| AGAATTGTAACATCAAATGG+AGG | + | chr8_2:9645982-9646001 | Msa1233010:CDS | 30.0% | |
| !!! | AGTGTGTCAAGTAGTTTTAA+AGG | + | chr8_2:9646459-9646478 | Msa1233010:CDS | 30.0% |
| ATGGGTAATTGCGTATTCAA+AGG | + | chr8_2:9645925-9645944 | Msa1233010:CDS | 35.0% | |
| GTATTCAAAGGCTTTCATCA+TGG | + | chr8_2:9645937-9645956 | Msa1233010:CDS | 35.0% | |
| GGAACTTTATTCTCCCATAA+CGG | + | chr8_2:9646011-9646030 | Msa1233010:CDS | 35.0% | |
| GTTATGCATTCAACCGTTAT+GGG | - | chr8_2:9646027-9646046 | None:intergenic | 35.0% | |
| !!! | AGTTCTTCGTTTTTTGACAG+TGG | - | chr8_2:9646105-9646124 | None:intergenic | 35.0% |
| AAAAACGAAGAACTTCAAGC+TGG | + | chr8_2:9646111-9646130 | Msa1233010:CDS | 35.0% | |
| !! | AACATCAAATGGAGGCATCA+TGG | + | chr8_2:9645990-9646009 | Msa1233010:CDS | 40.0% |
| GGTTATGCATTCAACCGTTA+TGG | - | chr8_2:9646028-9646047 | None:intergenic | 40.0% | |
| ! | GTGGTTTAGAGAGTGTGTTA+CGG | - | chr8_2:9646086-9646105 | None:intergenic | 40.0% |
| !! | CAATATTTTGGAGAGGGAGA+AGG | - | chr8_2:9646149-9646168 | None:intergenic | 40.0% |
| TCACATGTGGACATTCGATA+CGG | - | chr8_2:9646207-9646226 | None:intergenic | 40.0% | |
| ! | GTTGCTATTCTTGTCACATG+TGG | - | chr8_2:9646220-9646239 | None:intergenic | 40.0% |
| TTTCCGAGGTACAATAGTAG+TGG | + | chr8_2:9646279-9646298 | Msa1233010:CDS | 40.0% | |
| ! | GGTGAAATTGGTGATAAGTC+CGG | + | chr8_2:9646311-9646330 | Msa1233010:CDS | 40.0% |
| AAAATCTCCGACAACTTCTC+CGG | - | chr8_2:9646333-9646352 | None:intergenic | 40.0% | |
| ACTTGACACACTCCATTGAT+CGG | - | chr8_2:9646451-9646470 | None:intergenic | 40.0% | |
| ACGCCACTACTATTGTACCT+CGG | - | chr8_2:9646285-9646304 | None:intergenic | 45.0% | |
| GATAAGTCCGGAGAAGTTGT+CGG | + | chr8_2:9646323-9646342 | Msa1233010:CDS | 45.0% | |
| ! | AGATTTTGTCGCAAGAGTCG+AGG | + | chr8_2:9646346-9646365 | Msa1233010:CDS | 45.0% |
| ! | GTTGATAGAGAGTGTGAGGA+CGG | + | chr8_2:9646377-9646396 | Msa1233010:CDS | 45.0% |
| TTTGCCACCGAAGATGATGA+AGG | - | chr8_2:9646426-9646445 | None:intergenic | 45.0% | |
| ACCAACGAGTTCCCACATCA+TGG | + | chr8_2:9646045-9646064 | Msa1233010:CDS | 50.0% | |
| TCCATGATGTGGGAACTCGT+TGG | - | chr8_2:9646049-9646068 | None:intergenic | 50.0% | |
| ! | CGGAACATGAAGTGTTTCCG+AGG | + | chr8_2:9646265-9646284 | Msa1233010:CDS | 50.0% |
| GGTACAATAGTAGTGGCGTG+TGG | + | chr8_2:9646286-9646305 | Msa1233010:CDS | 50.0% | |
| CAATAGTAGTGGCGTGTGGA+AGG | + | chr8_2:9646290-9646309 | Msa1233010:CDS | 50.0% | |
| TGGCGTGTGGAAGGTGAAAT+TGG | + | chr8_2:9646299-9646318 | Msa1233010:CDS | 50.0% | |
| TTTGTCGCAAGAGTCGAGGA+CGG | + | chr8_2:9646350-9646369 | Msa1233010:CDS | 50.0% | |
| !! | AGGCGTTGATAGAGAGTGTG+AGG | + | chr8_2:9646373-9646392 | Msa1233010:CDS | 50.0% |
| ! | GATAGAGAGTGTGAGGACGG+TGG | + | chr8_2:9646380-9646399 | Msa1233010:CDS | 55.0% |
| ACGGTGGCTAAGTGCGGTAA+CGG | + | chr8_2:9646396-9646415 | Msa1233010:CDS | 55.0% | |
| ! | CGGGGTGCCTTCATCATCTT+CGG | + | chr8_2:9646416-9646435 | Msa1233010:CDS | 55.0% |
| GGTGCCTTCATCATCTTCGG+TGG | + | chr8_2:9646419-9646438 | Msa1233010:CDS | 55.0% | |
| CGGTGGCAAACTCCGATCAA+TGG | + | chr8_2:9646436-9646455 | Msa1233010:CDS | 55.0% | |
| GTGAGGACGGTGGCTAAGTG+CGG | + | chr8_2:9646390-9646409 | Msa1233010:CDS | 60.0% | |
| CGGTGGCTAAGTGCGGTAAC+GGG | + | chr8_2:9646397-9646416 | Msa1233010:CDS | 60.0% | |
| GGTGGCTAAGTGCGGTAACG+GGG | + | chr8_2:9646398-9646417 | Msa1233010:CDS | 60.0% | |
| GTCGCAAGAGTCGAGGACGG+AGG | + | chr8_2:9646353-9646372 | Msa1233010:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 9645925 | 9646530 | 9645925 | ID=Msa1233010;Name=Msa1233010 |
| chr8_2 | mRNA | 9645925 | 9646530 | 9645925 | ID=Msa1233010-mRNA-1;Parent=Msa1233010;Name=Msa1233010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201 |
| chr8_2 | exon | 9645925 | 9646530 | 9645925 | ID=Msa1233010-mRNA-1:exon:3804;Parent=Msa1233010-mRNA-1 |
| chr8_2 | CDS | 9645925 | 9646530 | 9645925 | ID=Msa1233010-mRNA-1:cds;Parent=Msa1233010-mRNA-1 |
| Gene Sequence |
| Protein sequence |
