AlfalfaGEDB | Msa1233010

M. sativa cultivar zhongmu-4 / Msa1233010

KEGG: -
GO: -
PFAM: PF14009.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1233010	XP_003627099.1	98.515	202	2	1	1	201	1	202	1.28e-141	403

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1233010	G7LHF9	98.515	202	2	1	1	201	1	202	6.09e-142	403

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0427700	Msa1233010	0.802060	6.709289e-49	-8.615850e-47
Msa1233010	Msa1275450	0.800869	1.177126e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1233010	MtrunA17_Chr8g0342361	98.515	202	2	1	1	201	1	202	1.17e-145	403
Msa1233010	MtrunA17_Chr1g0202491	43.000	200	87	6	1	181	1	192	2.68e-42	141
Msa1233010	MtrunA17_Chr6g0469901	35.827	254	98	8	1	196	1	247	4.52e-35	124
Msa1233010	MtrunA17_Chr8g0388471	31.148	183	89	8	1	160	1	169	2.09e-14	69.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1233010	AT3G61920.1	45.960	198	81	6	1	186	1	184	9.23e-42	139
Msa1233010	AT1G64700.1	36.559	186	90	7	1	161	1	183	2.59e-28	105

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Find 40 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTCCGACAACTTCTC+CGG	0.445716	8_2:-9646330	None:intergenic
AACATCAAATGGAGGCATCA+TGG	0.506675	8_2:+9645990	Msa1233010:CDS
ACAATAACAATAACAATATT+TGG	0.279085	8_2:+9646241	Msa1233010:CDS
ACCAACGAGTTCCCACATCA+TGG	0.565815	8_2:+9646045	Msa1233010:CDS
ACGCCACTACTATTGTACCT+CGG	0.689786	8_2:-9646282	None:intergenic
ACGGTGGCTAAGTGCGGTAA+CGG	0.449663	8_2:+9646396	Msa1233010:CDS
ACTTGACACACTCCATTGAT+CGG	0.607173	8_2:-9646448	None:intergenic
AGAATTGTAACATCAAATGG+AGG	0.652719	8_2:+9645982	Msa1233010:CDS
AGGCGTTGATAGAGAGTGTG+AGG	0.660586	8_2:+9646373	Msa1233010:CDS
ATGGGTAATTGCGTATTCAA+AGG	0.536080	8_2:+9645925	Msa1233010:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAATTGTAACATCAAATGG+AGG	+	chr8_2:9645982-9646001	Msa1233010:CDS	30.0%
ATGGGTAATTGCGTATTCAA+AGG	+	chr8_2:9645925-9645944	Msa1233010:CDS	35.0%
GTATTCAAAGGCTTTCATCA+TGG	+	chr8_2:9645937-9645956	Msa1233010:CDS	35.0%
GGAACTTTATTCTCCCATAA+CGG	+	chr8_2:9646011-9646030	Msa1233010:CDS	35.0%
GTTATGCATTCAACCGTTAT+GGG	-	chr8_2:9646027-9646046	None:intergenic	35.0%
AAAAACGAAGAACTTCAAGC+TGG	+	chr8_2:9646111-9646130	Msa1233010:CDS	35.0%
GGTTATGCATTCAACCGTTA+TGG	-	chr8_2:9646028-9646047	None:intergenic	40.0%
TCACATGTGGACATTCGATA+CGG	-	chr8_2:9646207-9646226	None:intergenic	40.0%
TTTCCGAGGTACAATAGTAG+TGG	+	chr8_2:9646279-9646298	Msa1233010:CDS	40.0%
AAAATCTCCGACAACTTCTC+CGG	-	chr8_2:9646333-9646352	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	9645925	9646530	9645925	ID=Msa1233010;Name=Msa1233010
chr8_2	mRNA	9645925	9646530	9645925	ID=Msa1233010-mRNA-1;Parent=Msa1233010;Name=Msa1233010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|201
chr8_2	exon	9645925	9646530	9645925	ID=Msa1233010-mRNA-1:exon:3804;Parent=Msa1233010-mRNA-1
chr8_2	CDS	9645925	9646530	9645925	ID=Msa1233010-mRNA-1:cds;Parent=Msa1233010-mRNA-1

Gene Sequence

>Msa1233010

ATGGGTAATTGCGTATTCAAAGGCTTTCATCATGGAGATTTTGAAGAAACAATGGTAAGAATTGTAACATCAAATGGAGGCATCATGGAACTTTATTCTCCCATAACGGTTGAATGCATAACCAACGAGTTCCCACATCATGGAATTTTCAAAAACAACCGTAACACACTCTCTAAACCACTGTCAAAAAACGAAGAACTTCAAGCTGGTGAAATTTACTACCTTCTCCCTCTCCAAAATATTGTTAAACAATTTGGTGAAACATTCGAAACGTTAACTCCGTATCGAATGTCCACATGTGACAAGAATAGCAACAACAATAACAATAACAATATTTGGTCGGAACATGAAGTGTTTCCGAGGTACAATAGTAGTGGCGTGTGGAAGGTGAAATTGGTGATAAGTCCGGAGAAGTTGTCGGAGATTTTGTCGCAAGAGTCGAGGACGGAGGCGTTGATAGAGAGTGTGAGGACGGTGGCTAAGTGCGGTAACGGGGTGCCTTCATCATCTTCGGTGGCAAACTCCGATCAATGGAGTGTGTCAAGTAGTTTTAAAGGTTCTACGTTAATTGACAAATTTAATTTAGAATCTTCAAGTTCAAATTAG

Protein sequence

>Msa1233010

MGNCVFKGFHHGDFEETMVRIVTSNGGIMELYSPITVECITNEFPHHGIFKNNRNTLSKPLSKNEELQAGEIYYLLPLQNIVKQFGETFETLTPYRMSTCDKNSNNNNNNNIWSEHEVFPRYNSSGVWKVKLVISPEKLSEILSQESRTEALIESVRTVAKCGNGVPSSSSVANSDQWSVSSSFKGSTLIDKFNLESSSSN*