Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1275450 | G7LHF9 | 99.010 | 202 | 1 | 1 | 1 | 201 | 1 | 202 | 2.03e-142 | 404 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0106710 | Msa1275450 | 0.803469 | 3.434688e-49 | -8.615850e-47 |
| Msa0108410 | Msa1275450 | 0.812128 | 4.955083e-51 | -8.615850e-47 |
| Msa0624420 | Msa1275450 | 0.802305 | 5.975169e-49 | -8.615850e-47 |
| Msa0657660 | Msa1275450 | 0.808997 | 2.352097e-50 | -8.615850e-47 |
| Msa0664540 | Msa1275450 | 0.803911 | 2.780852e-49 | -8.615850e-47 |
| Msa0693330 | Msa1275450 | 0.820993 | 5.113531e-53 | -8.615850e-47 |
| Msa0702720 | Msa1275450 | 0.827685 | 1.364701e-54 | -8.615850e-47 |
| Msa0715060 | Msa1275450 | 0.807655 | 4.545761e-50 | -8.615850e-47 |
| Msa0833530 | Msa1275450 | 0.803819 | 2.905231e-49 | -8.615850e-47 |
| Msa0207350 | Msa1275450 | 0.814908 | 1.212273e-51 | -8.615850e-47 |
| Msa0221000 | Msa1275450 | 0.800910 | 1.154841e-48 | -8.615850e-47 |
| Msa1233010 | Msa1275450 | 0.800869 | 1.177126e-48 | -8.615850e-47 |
| Msa1275450 | Msa1340200 | 0.802372 | 5.789372e-49 | -8.615850e-47 |
| Msa1275450 | Msa1341840 | 0.819975 | 8.757933e-53 | -8.615850e-47 |
| Msa1275450 | Msa1418240 | 0.821849 | 3.243903e-53 | -8.615850e-47 |
| Msa1275450 | Msa1461370 | 0.813437 | 2.561375e-51 | -8.615850e-47 |
| Msa0378720 | Msa1275450 | 0.801073 | 1.069357e-48 | -8.615850e-47 |
| Msa0427100 | Msa1275450 | 0.803653 | 3.146054e-49 | -8.615850e-47 |
| Msa0427120 | Msa1275450 | 0.802565 | 5.280887e-49 | -8.615850e-47 |
| Msa0450400 | Msa1275450 | -0.802181 | 6.337742e-49 | -8.615850e-47 |
| Msa1006810 | Msa1275450 | 0.827621 | 1.413937e-54 | -8.615850e-47 |
| Msa1034500 | Msa1275450 | 0.811754 | 5.976379e-51 | -8.615850e-47 |
| Msa1061980 | Msa1275450 | 0.805115 | 1.559725e-49 | -8.615850e-47 |
| Msa1089450 | Msa1275450 | 0.814977 | 1.170081e-51 | -8.615850e-47 |
| Msa0240900 | Msa1275450 | 0.802190 | 6.310722e-49 | -8.615850e-47 |
| Msa0279850 | Msa1275450 | 0.801557 | 8.511329e-49 | -8.615850e-47 |
| Msa0300320 | Msa1275450 | 0.803706 | 3.067544e-49 | -8.615850e-47 |
| Msa0317970 | Msa1275450 | 0.811785 | 5.884499e-51 | -8.615850e-47 |
| Msa0322900 | Msa1275450 | 0.817363 | 3.427741e-52 | -8.615850e-47 |
| Msa0341690 | Msa1275450 | 0.804893 | 1.736197e-49 | -8.615850e-47 |
| Msa0485240 | Msa1275450 | 0.825994 | 3.460591e-54 | -8.615850e-47 |
| Msa0488260 | Msa1275450 | 0.808518 | 2.977149e-50 | -8.615850e-47 |
| Msa0601950 | Msa1275450 | 0.813063 | 3.093228e-51 | -8.615850e-47 |
| Msa0855870 | Msa1275450 | 0.801975 | 6.987470e-49 | -8.615850e-47 |
| Msa0870520 | Msa1275450 | 0.826362 | 2.828597e-54 | -8.615850e-47 |
| Msa0870540 | Msa1275450 | 0.818645 | 1.759113e-52 | -8.615850e-47 |
| Msa0871720 | Msa1275450 | 0.806261 | 8.962940e-50 | -8.615850e-47 |
| Msa0903710 | Msa1275450 | 0.808208 | 3.467846e-50 | -8.615850e-47 |
| Msa0936380 | Msa1275450 | 0.804503 | 2.093808e-49 | -8.615850e-47 |
| Msa0937020 | Msa1275450 | 0.807373 | 5.216431e-50 | -8.615850e-47 |
| Msa0973140 | Msa1275450 | 0.821264 | 4.429678e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1275450 | MtrunA17_Chr8g0342361 | 99.010 | 202 | 1 | 1 | 1 | 201 | 1 | 202 | 3.91e-146 | 404 |
| Msa1275450 | MtrunA17_Chr1g0202491 | 43.000 | 200 | 87 | 6 | 1 | 181 | 1 | 192 | 2.80e-42 | 141 |
| Msa1275450 | MtrunA17_Chr6g0469901 | 35.827 | 254 | 98 | 8 | 1 | 196 | 1 | 247 | 4.11e-35 | 124 |
| Msa1275450 | MtrunA17_Chr8g0388471 | 33.880 | 183 | 84 | 6 | 1 | 160 | 1 | 169 | 4.05e-14 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1275450 | AT3G61920.1 | 45.960 | 198 | 81 | 6 | 1 | 186 | 1 | 184 | 8.94e-42 | 139 |
| Msa1275450 | AT1G64700.1 | 37.097 | 186 | 89 | 7 | 1 | 161 | 1 | 183 | 1.88e-28 | 106 |
Find 38 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAATAACAATAACAATATT+TGG | 0.279085 | 8_3:-9388180 | Msa1275450:CDS |
| GGTTATGCATTCAACCGTTA+TGG | 0.318183 | 8_3:+9388396 | None:intergenic |
| CGGTGGCTAAGTGTGGTAAC+GGG | 0.373321 | 8_3:-9388024 | Msa1275450:CDS |
| TGGAGTGTGGAAGGTGAAAT+TGG | 0.387323 | 8_3:-9388122 | Msa1275450:CDS |
| GTAAGAATTGTAACATCAAA+TGG | 0.436383 | 8_3:-9388442 | Msa1275450:CDS |
| AAAATCTCCGACAACTTCTC+CGG | 0.445716 | 8_3:+9388091 | None:intergenic |
| GTTATGCATTCAACCGTTAT+GGG | 0.458911 | 8_3:+9388397 | None:intergenic |
| CGGTGGCAAACTCCGATCAA+TGG | 0.497676 | 8_3:-9387985 | Msa1275450:CDS |
| AACATCAAATGGAGGCATCA+TGG | 0.506675 | 8_3:-9388431 | Msa1275450:CDS |
| CAATAGTAGTGGAGTGTGGA+AGG | 0.512822 | 8_3:-9388131 | Msa1275450:CDS |
| GGAACTTTATTCTCCCATAA+CGG | 0.519483 | 8_3:-9388410 | Msa1275450:CDS |
| GGTGAAATTGGTGATAAGTC+CGG | 0.528772 | 8_3:-9388110 | Msa1275450:CDS |
| ATGGGTAATTGCGTATTCAA+AGG | 0.536080 | 8_3:-9388496 | Msa1275450:CDS |
| CGGGGTGCCTTCATCGTCAT+CGG | 0.542605 | 8_3:-9388005 | Msa1275450:CDS |
| CACACTCTCTATCAATGCCT+CGG | 0.551049 | 8_3:+9388051 | None:intergenic |
| ACTTGACACGCTCCATTGAT+CGG | 0.555748 | 8_3:+9387973 | None:intergenic |
| ACGGTGGCTAAGTGTGGTAA+CGG | 0.556735 | 8_3:-9388025 | Msa1275450:CDS |
| GTATTCAAAGGCTTTCATCA+TGG | 0.561060 | 8_3:-9388484 | Msa1275450:CDS |
| ACCAACGAGTTCCCACATCA+TGG | 0.565815 | 8_3:-9388376 | Msa1275450:CDS |
| GTTGCTATTCTTGTCACATG+TGG | 0.579217 | 8_3:+9388204 | None:intergenic |
| GTGGTTTAGAGAGTGTGTTA+CGG | 0.579924 | 8_3:+9388338 | None:intergenic |
| GATAAGTCCGGAGAAGTTGT+CGG | 0.582804 | 8_3:-9388098 | Msa1275450:CDS |
| TAACAATAACAATATTTGGT+CGG | 0.587255 | 8_3:-9388176 | Msa1275450:CDS |
| TTTGCCACCGATGACGATGA+AGG | 0.593281 | 8_3:+9387998 | None:intergenic |
| TCACATGTGGACATTCGATA+CGG | 0.603282 | 8_3:+9388217 | None:intergenic |
| TTTCCGAGGTACAATAGTAG+TGG | 0.606512 | 8_3:-9388142 | Msa1275450:CDS |
| GGTGGCTAAGTGTGGTAACG+GGG | 0.606842 | 8_3:-9388023 | Msa1275450:CDS |
| GGTGCCTTCATCGTCATCGG+TGG | 0.616262 | 8_3:-9388002 | Msa1275450:CDS |
| ATTGATAGAGAGTGTGAGGA+CGG | 0.627000 | 8_3:-9388044 | Msa1275450:CDS |
| AGGCATTGATAGAGAGTGTG+AGG | 0.645919 | 8_3:-9388048 | Msa1275450:CDS |
| TCCATGATGTGGGAACTCGT+TGG | 0.650105 | 8_3:+9388375 | None:intergenic |
| GGTACAATAGTAGTGGAGTG+TGG | 0.650966 | 8_3:-9388135 | Msa1275450:CDS |
| AGAATTGTAACATCAAATGG+AGG | 0.652719 | 8_3:-9388439 | Msa1275450:CDS |
| GTCGCAAGAGTCGAGGACCG+AGG | 0.657790 | 8_3:-9388068 | Msa1275450:CDS |
| CGGAACATGAAGTGTTTCCG+AGG | 0.663787 | 8_3:-9388156 | Msa1275450:CDS |
| GTGAGGACGGTGGCTAAGTG+TGG | 0.679079 | 8_3:-9388031 | Msa1275450:CDS |
| GATAGAGAGTGTGAGGACGG+TGG | 0.695119 | 8_3:-9388041 | Msa1275450:CDS |
| ACTCCACTACTATTGTACCT+CGG | 0.708316 | 8_3:+9388139 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACATTGTTAAACAATT+TGG | - | chr8_3:9388147-9388166 | Msa1275450:CDS | 15.0% |
| !! | ACAATAACAATAACAATATT+TGG | - | chr8_3:9388229-9388248 | Msa1275450:CDS | 15.0% |
| !! | TAACAATAACAATATTTGGT+CGG | - | chr8_3:9388233-9388252 | Msa1275450:CDS | 20.0% |
| ! | GTAAGAATTGTAACATCAAA+TGG | - | chr8_3:9387967-9387986 | Msa1275450:CDS | 25.0% |
| !!! | TTTTGAAAATTCCATGATGT+GGG | + | chr8_3:9388047-9388066 | None:intergenic | 25.0% |
| !!! | TTTTTGAAAATTCCATGATG+TGG | + | chr8_3:9388048-9388067 | None:intergenic | 25.0% |
| !!! | TGTTTAACAATGTTTTTGAG+AGG | + | chr8_3:9388144-9388163 | None:intergenic | 25.0% |
| !! | TGGAGATTTTGAAGAAACAA+TGG | - | chr8_3:9387945-9387964 | Msa1275450:CDS | 30.0% |
| AGAATTGTAACATCAAATGG+AGG | - | chr8_3:9387970-9387989 | Msa1275450:CDS | 30.0% | |
| ATGGGTAATTGCGTATTCAA+AGG | - | chr8_3:9387913-9387932 | Msa1275450:CDS | 35.0% | |
| GTATTCAAAGGCTTTCATCA+TGG | - | chr8_3:9387925-9387944 | Msa1275450:CDS | 35.0% | |
| GGAACTTTATTCTCCCATAA+CGG | - | chr8_3:9387999-9388018 | Msa1275450:CDS | 35.0% | |
| GTTATGCATTCAACCGTTAT+GGG | + | chr8_3:9388015-9388034 | None:intergenic | 35.0% | |
| !!! | AGTTCTTCGTTTTTTGAGAG+TGG | + | chr8_3:9388093-9388112 | None:intergenic | 35.0% |
| AAAAACGAAGAACTTCAAGC+TGG | - | chr8_3:9388099-9388118 | Msa1275450:CDS | 35.0% | |
| !! | CAATGTTTTTGAGAGGAAGA+AGG | + | chr8_3:9388137-9388156 | None:intergenic | 35.0% |
| !!! | AGCGTGTCAAGTAGTTTTAA+AGG | - | chr8_3:9388447-9388466 | Msa1275450:CDS | 35.0% |
| !! | AACATCAAATGGAGGCATCA+TGG | - | chr8_3:9387978-9387997 | Msa1275450:CDS | 40.0% |
| GGTTATGCATTCAACCGTTA+TGG | + | chr8_3:9388016-9388035 | None:intergenic | 40.0% | |
| ! | GTGGTTTAGAGAGTGTGTTA+CGG | + | chr8_3:9388074-9388093 | None:intergenic | 40.0% |
| TCACATGTGGACATTCGATA+CGG | + | chr8_3:9388195-9388214 | None:intergenic | 40.0% | |
| ! | GTTGCTATTCTTGTCACATG+TGG | + | chr8_3:9388208-9388227 | None:intergenic | 40.0% |
| TTTCCGAGGTACAATAGTAG+TGG | - | chr8_3:9388267-9388286 | Msa1275450:CDS | 40.0% | |
| ACTCCACTACTATTGTACCT+CGG | + | chr8_3:9388273-9388292 | None:intergenic | 40.0% | |
| ! | GGTGAAATTGGTGATAAGTC+CGG | - | chr8_3:9388299-9388318 | Msa1275450:CDS | 40.0% |
| AAAATCTCCGACAACTTCTC+CGG | + | chr8_3:9388321-9388340 | None:intergenic | 40.0% | |
| ! | ATTGATAGAGAGTGTGAGGA+CGG | - | chr8_3:9388365-9388384 | Msa1275450:CDS | 40.0% |
| ! | GGTACAATAGTAGTGGAGTG+TGG | - | chr8_3:9388274-9388293 | Msa1275450:CDS | 45.0% |
| ! | CAATAGTAGTGGAGTGTGGA+AGG | - | chr8_3:9388278-9388297 | Msa1275450:CDS | 45.0% |
| TGGAGTGTGGAAGGTGAAAT+TGG | - | chr8_3:9388287-9388306 | Msa1275450:CDS | 45.0% | |
| GATAAGTCCGGAGAAGTTGT+CGG | - | chr8_3:9388311-9388330 | Msa1275450:CDS | 45.0% | |
| ! | AGATTTTGTCGCAAGAGTCG+AGG | - | chr8_3:9388334-9388353 | Msa1275450:CDS | 45.0% |
| CACACTCTCTATCAATGCCT+CGG | + | chr8_3:9388361-9388380 | None:intergenic | 45.0% | |
| !! | AGGCATTGATAGAGAGTGTG+AGG | - | chr8_3:9388361-9388380 | Msa1275450:CDS | 45.0% |
| ACTTGACACGCTCCATTGAT+CGG | + | chr8_3:9388439-9388458 | None:intergenic | 45.0% | |
| ACCAACGAGTTCCCACATCA+TGG | - | chr8_3:9388033-9388052 | Msa1275450:CDS | 50.0% | |
| TCCATGATGTGGGAACTCGT+TGG | + | chr8_3:9388037-9388056 | None:intergenic | 50.0% | |
| ! | CGGAACATGAAGTGTTTCCG+AGG | - | chr8_3:9388253-9388272 | Msa1275450:CDS | 50.0% |
| ACGGTGGCTAAGTGTGGTAA+CGG | - | chr8_3:9388384-9388403 | Msa1275450:CDS | 50.0% | |
| TTTGCCACCGATGACGATGA+AGG | + | chr8_3:9388414-9388433 | None:intergenic | 50.0% | |
| ! | GATAGAGAGTGTGAGGACGG+TGG | - | chr8_3:9388368-9388387 | Msa1275450:CDS | 55.0% |
| CGGTGGCTAAGTGTGGTAAC+GGG | - | chr8_3:9388385-9388404 | Msa1275450:CDS | 55.0% | |
| ! | GGTGGCTAAGTGTGGTAACG+GGG | - | chr8_3:9388386-9388405 | Msa1275450:CDS | 55.0% |
| CGGTGGCAAACTCCGATCAA+TGG | - | chr8_3:9388424-9388443 | Msa1275450:CDS | 55.0% | |
| GTGAGGACGGTGGCTAAGTG+TGG | - | chr8_3:9388378-9388397 | Msa1275450:CDS | 60.0% | |
| ! | CGGGGTGCCTTCATCGTCAT+CGG | - | chr8_3:9388404-9388423 | Msa1275450:CDS | 60.0% |
| GGTGCCTTCATCGTCATCGG+TGG | - | chr8_3:9388407-9388426 | Msa1275450:CDS | 60.0% | |
| GTCGCAAGAGTCGAGGACCG+AGG | - | chr8_3:9388341-9388360 | Msa1275450:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 9387913 | 9388518 | 9387913 | ID=Msa1275450;Name=Msa1275450 |
| chr8_3 | mRNA | 9387913 | 9388518 | 9387913 | ID=Msa1275450-mRNA-1;Parent=Msa1275450;Name=Msa1275450-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|201 |
| chr8_3 | exon | 9387913 | 9388518 | 9387913 | ID=Msa1275450-mRNA-1:exon:3942;Parent=Msa1275450-mRNA-1 |
| chr8_3 | CDS | 9387913 | 9388518 | 9387913 | ID=Msa1275450-mRNA-1:cds;Parent=Msa1275450-mRNA-1 |
| Gene Sequence |
| Protein sequence |
