AlfalfaGEDB | Msa1233430

M. sativa cultivar zhongmu-4 / Msa1233430

KEGG: K02218
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1233430	XP_003627159.1	99.578	474	2	0	1	474	1	474	0.0	973

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1233430	sp\|Q9CAI5\|CKL2_ARATH	76.170	470	104	6	1	467	1	465	0.0	709

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1233430	G7LI85	99.578	474	2	0	1	474	1	474	0.0	973

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1195260	Msa1233430	0.997545	1.749888e-244	-8.615850e-47
Msa1233430	Msa1275740	0.845334	4.334096e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1233430	MtrunA17_Chr8g0342821	99.578	474	2	0	1	474	1	474	0.0	973
Msa1233430	MtrunA17_Chr2g0329981	83.438	477	74	5	1	474	1	475	0.0	809
Msa1233430	MtrunA17_Chr4g0071031	75.314	478	90	7	1	474	1	454	0.0	690
Msa1233430	MtrunA17_Chr2g0299661	68.723	470	142	4	1	469	1	466	0.0	653
Msa1233430	MtrunA17_Chr1g0183631	60.000	475	155	8	1	465	1	450	0.0	547
Msa1233430	MtrunA17_Chr7g0261501	77.538	325	64	3	1	318	1	323	0.0	540
Msa1233430	MtrunA17_Chr1g0166821	79.180	317	63	2	1	314	2	318	0.0	540
Msa1233430	MtrunA17_Chr1g0187571	79.798	297	59	1	1	296	2	298	0.0	519
Msa1233430	MtrunA17_Chr6g0486611	77.926	299	65	1	1	298	1	299	1.04e-178	507
Msa1233430	MtrunA17_Chr8g0390211	76.589	299	69	1	1	298	1	299	1.41e-174	496

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1233430	AT1G72710.1	76.170	470	104	6	1	467	1	465	709
Msa1233430	AT4G26100.1	69.979	473	112	9	1	470	1	446	652
Msa1233430	AT5G57015.1	69.538	476	101	9	1	474	1	434	644
Msa1233430	AT2G19470.1	69.533	407	102	5	1	404	1	388	573
Msa1233430	AT1G03930.1	60.970	474	173	6	1	467	1	469	542
Msa1233430	AT5G44100.1	82.492	297	51	1	1	296	1	297	539
Msa1233430	AT5G44100.2	82.492	297	51	1	1	296	1	297	539
Msa1233430	AT3G23340.1	64.096	415	135	6	1	414	1	402	531
Msa1233430	AT4G28540.1	73.620	326	78	2	1	318	5	330	520
Msa1233430	AT4G14340.1	63.260	411	137	5	1	410	7	404	519

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Find 148 sgRNAs with CRISPR-Local

Find 319 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGGTAGCGGATCTTT+TGG	0.312915	8_2:-10206765	Msa1233430:CDS
AAACAAGGCCCAAACCTTCA+CGG	0.553845	8_2:+10203745	None:intergenic
AAACCCATGAGGAAATTGTC+TGG	0.531680	8_2:+10205476	None:intergenic
AAACTTGAATCCAGCGTGAG+AGG	0.575553	8_2:+10202705	None:intergenic
AAAGCCAGACAATTTCCTCA+TGG	0.544831	8_2:-10205480	Msa1233430:CDS
AACAAAACTAGCCTCGTAAA+AGG	0.358968	8_2:-10202336	Msa1233430:three_prime_UTR
AACCGGATCTAAAGATGCTA+TGG	0.540198	8_2:-10203007	Msa1233430:intron
AAGAAAACAAATGATAGTAA+AGG	0.529240	8_2:+10202269	None:intergenic
AAGAAGAAAAGGGTATATCT+GGG	0.527099	8_2:+10206890	None:intergenic
AAGATGGTAGGAATATTGGG+TGG	0.729848	8_2:-10203122	Msa1233430:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTCCAAAAAACAGCGTT+AGG	-	chr8_2:10201856-10201875	Msa1233430:three_prime_UTR	30.0%
GAAAAAGGAAAGGACAAAAT+AGG	+	chr8_2:10201972-10201991	None:intergenic	30.0%
GTTATTGAAGTTTGATGAGA+AGG	-	chr8_2:10202383-10202402	Msa1233430:three_prime_UTR	30.0%
TATTGAAGTTTGATGAGAAG+GGG	-	chr8_2:10202385-10202404	Msa1233430:three_prime_UTR	30.0%
TAATCGATACTTCGATTACA+AGG	+	chr8_2:10202550-10202569	None:intergenic	30.0%
AGGTAATTAGATGATTGATC+AGG	-	chr8_2:10202577-10202596	Msa1233430:CDS	30.0%
TGATTAACCAAGTCTGATTA+TGG	+	chr8_2:10202618-10202637	None:intergenic	30.0%
TTGCTTCATACAACAATTGA+GGG	+	chr8_2:10202971-10202990	None:intergenic	30.0%
TTTGCTTCATACAACAATTG+AGG	+	chr8_2:10202972-10202991	None:intergenic	30.0%
AAGCTGTATAAAATACTGCA+AGG	-	chr8_2:10202990-10203009	Msa1233430:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	10201799	10207112	10201799	ID=Msa1233430;Name=Msa1233430
chr8_2	mRNA	10201799	10207112	10201799	ID=Msa1233430-mRNA-1;Parent=Msa1233430;Name=Msa1233430-mRNA-1;_AED=0.18;_eAED=0.18;_QI=286\|1\|1\|1\|1\|1\|14\|740\|474
chr8_2	exon	10201799	10202832	10201799	ID=Msa1233430-mRNA-1:exon:4032;Parent=Msa1233430-mRNA-1
chr8_2	exon	10203008	10203153	10203008	ID=Msa1233430-mRNA-1:exon:4031;Parent=Msa1233430-mRNA-1
chr8_2	exon	10203335	10203398	10203335	ID=Msa1233430-mRNA-1:exon:4030;Parent=Msa1233430-mRNA-1
chr8_2	exon	10203494	10203576	10203494	ID=Msa1233430-mRNA-1:exon:4029;Parent=Msa1233430-mRNA-1
chr8_2	exon	10203760	10203886	10203760	ID=Msa1233430-mRNA-1:exon:4028;Parent=Msa1233430-mRNA-1
chr8_2	exon	10203984	10204068	10203984	ID=Msa1233430-mRNA-1:exon:4027;Parent=Msa1233430-mRNA-1
chr8_2	exon	10204150	10204213	10204150	ID=Msa1233430-mRNA-1:exon:4026;Parent=Msa1233430-mRNA-1
chr8_2	exon	10204301	10204362	10204301	ID=Msa1233430-mRNA-1:exon:4025;Parent=Msa1233430-mRNA-1
chr8_2	exon	10205291	10205358	10205291	ID=Msa1233430-mRNA-1:exon:4024;Parent=Msa1233430-mRNA-1
chr8_2	exon	10205457	10205552	10205457	ID=Msa1233430-mRNA-1:exon:4023;Parent=Msa1233430-mRNA-1
chr8_2	exon	10205634	10205782	10205634	ID=Msa1233430-mRNA-1:exon:4022;Parent=Msa1233430-mRNA-1
chr8_2	exon	10205894	10205963	10205894	ID=Msa1233430-mRNA-1:exon:4021;Parent=Msa1233430-mRNA-1
chr8_2	exon	10206624	10206664	10206624	ID=Msa1233430-mRNA-1:exon:4020;Parent=Msa1233430-mRNA-1
chr8_2	exon	10206751	10207112	10206751	ID=Msa1233430-mRNA-1:exon:4019;Parent=Msa1233430-mRNA-1
chr8_2	five_prime_UTR	10206827	10207112	10206827	ID=Msa1233430-mRNA-1:five_prime_utr;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10206751	10206826	10206751	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10206624	10206664	10206624	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10205894	10205963	10205894	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10205634	10205782	10205634	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10205457	10205552	10205457	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10205291	10205358	10205291	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10204301	10204362	10204301	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10204150	10204213	10204150	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10203984	10204068	10203984	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10203760	10203886	10203760	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10203494	10203576	10203494	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10203335	10203398	10203335	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10203008	10203153	10203008	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	CDS	10202539	10202832	10202539	ID=Msa1233430-mRNA-1:cds;Parent=Msa1233430-mRNA-1
chr8_2	three_prime_UTR	10201799	10202538	10201799	ID=Msa1233430-mRNA-1:three_prime_utr;Parent=Msa1233430-mRNA-1

Gene Sequence

>Msa1233430

ATGGAACCTCGAATTGGAAATAAGTTCCGTCTTGGGAGAAAAATTGGTAGCGGATCTTTTGGAGAGATCTATTTAGGTACCAATATTCAGACAAATGAAGAAGTTGCGATTAAGCTTGAAAATGTTAAAACCAAGCACCCTCAATTGTTGTATGAAGCAAAGCTGTATAAAATACTGCAAGGAGGAACTGGAATACCGAATGTGAAATGGTCCGGTGTGGAAGGAGAGTACAACATCCTTGTGATGGATTTACTTGGCCCTAGTCTTGAGGATTTATTCAATTTCTGTAATAGGAAATTGTCTCTCAAAACCGTTCTTATGCTTGCCGATCAAATGATAAATCGAGTTGAATTTGTTCATACCAAGTCATTTTTACATCGGGACATAAAGCCAGACAATTTCCTCATGGGTTTAGGGAGGCGTGCAAATCAAGTGTACATCATTGACTTTGGTCTTGCTAAGAAATTCAGAGACAGTGCACATCAGCATATTCCCTATAGAGAGAATAAGAATCTGACGGGAACTGCTAGATATGCTAGTATGAATACTCATCTTGGAATTGAGCAAAGCCGTAGGGATGATTTAGAGTCGCTTGGATATGTTCTTATGTATTTTTTAAGAGGAAGTCTACCTTGGCAGGGATTGAAAGCAGGTACTAAAAAGCAGAAGTATGAGAGAATCAGTGAGAAGAAAGTTTCTACATCTATTGAGTCTCTCTGCCGTGGCTATCCCTCAGAATTTGCTTCATACTTCCATTATTGTCGGTCACTGAGGTTTGATGATAAGCCAGATTATGCTTATCTGAAAAGACTTTTGCGTGACCTATTCATCCGTGAAGGATTCCAGTTTGATTATGTCTTCGATTGGACTATCTTGAAATATCAGCAGTCTCAAATCGCCACTCCTCCTTCCCGTGGTATTGGTCTTGCTGCTGGACCGAGTTCTGGTCTGCCGCTAGCTTCTGCAAATGCTGATGGACAGTCAGGTGGTGGAAAAGATGGTAGGAATATTGGGTGGTCTTCGTCTGATCCCACTCGAAGGAGAACCTCTGGACCTATTGCTAATGATGGAATCTTATCTAGAGAAAAAGCCCCACTTACAAATGACTTAACCGGATCTAAAGATGCTATGTTATCGAGTTCGAATTTCTTTCGGTCAAGTGGATCTGCAAGGCGAGGTGCTATGTTAAGCAGCCGTGATGCAGCTGTTGGCAGTGAGACGGAGCCCTCCTCTCGTCCTCTCACGCTGGATTCAAGTTTAGGAGCAGTCCGTAAAAGCTCTGGTGCTCATAGAAGTTCACATATCATGTCATCGGAGCACAACCGAGTATCATCTGGAAGAAACACATCAAACATAAATAATTTGGATTCAACGCTTAGAGGTATTGAGAGTCTGCATTTAAACGATGAGAGGGCACAGTATTAG

Protein sequence

>Msa1233430

MEPRIGNKFRLGRKIGSGSFGEIYLGTNIQTNEEVAIKLENVKTKHPQLLYEAKLYKILQGGTGIPNVKWSGVEGEYNILVMDLLGPSLEDLFNFCNRKLSLKTVLMLADQMINRVEFVHTKSFLHRDIKPDNFLMGLGRRANQVYIIDFGLAKKFRDSAHQHIPYRENKNLTGTARYASMNTHLGIEQSRRDDLESLGYVLMYFLRGSLPWQGLKAGTKKQKYERISEKKVSTSIESLCRGYPSEFASYFHYCRSLRFDDKPDYAYLKRLLRDLFIREGFQFDYVFDWTILKYQQSQIATPPSRGIGLAAGPSSGLPLASANADGQSGGGKDGRNIGWSSSDPTRRRTSGPIANDGILSREKAPLTNDLTGSKDAMLSSSNFFRSSGSARRGAMLSSRDAAVGSETEPSSRPLTLDSSLGAVRKSSGAHRSSHIMSSEHNRVSSGRNTSNINNLDSTLRGIESLHLNDERAQY*