AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1235500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1235500 MtrunA17_Chr8g0345521 87.640 89 5 1 1 83 1 89 3.47e-48 147
Msa1235500 MtrunA17_Chr8g0345541 83.133 83 14 0 1 83 1 83 1.17e-42 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 101 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATGAACCTTCAAATATTT+TGG 0.096347 8_2:+13536389 Msa1235500:three_prime_UTR
ACTGATCCCACTCTTGATTT+TGG 0.147095 8_2:+13535854 Msa1235500:three_prime_UTR
CAATTTCCTTGTCATCAATT+TGG 0.159156 8_2:-13535057 None:intergenic
CATACGAACACCGCGGTATT+TGG 0.219306 8_2:-13535342 None:intergenic
TATGTCTTCTCAGTTTATTC+TGG 0.229280 8_2:+13534451 Msa1235500:exon
CGGTTTAACATTGACGATTT+CGG 0.262003 8_2:+13535890 Msa1235500:three_prime_UTR
GGCAATGGAGGCAATCAAAA+AGG 0.264426 8_2:+13535502 Msa1235500:three_prime_UTR
CTCAAGCTTATGAATCTAAA+AGG 0.275287 8_2:+13535456 Msa1235500:three_prime_UTR
CAATGCGATTGAGGCCTAAA+AGG 0.279868 8_2:+13534594 Msa1235500:CDS
TTTCTCCTTGATACAGTCTT+AGG 0.281725 8_2:-13535236 None:intergenic
TTCAAGGATATTCAAATTCA+TGG 0.318937 8_2:+13535947 Msa1235500:three_prime_UTR
TGTTACCGAGTTGTGTGTTT+TGG 0.333384 8_2:+13534772 Msa1235500:CDS
TTATTCTGGAATCAGTTGTT+GGG 0.348752 8_2:+13534465 Msa1235500:CDS
ATCAAACTTCTCAATCGAAT+TGG 0.353862 8_2:-13535696 None:intergenic
CTTTCAGAAATGTTAGAATC+TGG 0.376430 8_2:+13535402 Msa1235500:three_prime_UTR
TTTATTCTGGAATCAGTTGT+TGG 0.383668 8_2:+13534464 Msa1235500:CDS
ATCAATTTGGATTCTTTCAA+AGG 0.400070 8_2:-13535044 None:intergenic
GGGCTCTGTTGAAGAACCTC+TGG 0.401388 8_2:+13534486 Msa1235500:CDS
GGTGATGATGATGAATTTGC+TGG 0.406438 8_2:+13536022 Msa1235500:three_prime_UTR
ATTGTTGCTGAGAATCCAAT+TGG 0.407295 8_2:+13535830 Msa1235500:three_prime_UTR
AAGGTTGAAGTGTTTGTTGT+TGG 0.411506 8_2:+13534391 Msa1235500:intron
AAGTTTAAAACAGTCATATC+TGG 0.411631 8_2:-13535797 None:intergenic
AATGTATCCAAAATATTTGA+AGG 0.416141 8_2:-13536396 None:intergenic
TGGAGACTTCGGTGACTTCA+AGG 0.419980 8_2:+13535931 Msa1235500:three_prime_UTR
GCCTTCTTCCGTGCTTGAGT+TGG 0.420542 8_2:+13535661 Msa1235500:three_prime_UTR
CTGAACTTTACTATATTGTT+CGG 0.427450 8_2:+13536332 Msa1235500:three_prime_UTR
GTATGAGGCCATGGGGCAAA+TGG 0.449753 8_2:+13535360 Msa1235500:three_prime_UTR
CGAACAATATAGTAAAGTTC+AGG 0.452336 8_2:-13536331 None:intergenic
GTTGAAGTGTTTGTTGTTGG+AGG 0.456195 8_2:+13534394 Msa1235500:five_prime_UTR
ACAAGGTATGTTGTGTTGGC+GGG 0.457585 8_2:-13536059 None:intergenic
CAGAAATGTTAGAATCTGGT+TGG 0.458650 8_2:+13535406 Msa1235500:three_prime_UTR
AGAAATGTTAGAATCTGGTT+GGG 0.458838 8_2:+13535407 Msa1235500:three_prime_UTR
TGAACTTAAATTTCAGGCAA+TGG 0.461033 8_2:+13535487 Msa1235500:three_prime_UTR
GCGGTGTTCGTATGAGGCCA+TGG 0.462248 8_2:+13535351 Msa1235500:three_prime_UTR
TTCATTGACAGCAGCAGCCT+CGG 0.463601 8_2:-13535732 None:intergenic
ATCAAATCCAAAATCAAGAG+TGG 0.471193 8_2:-13535861 None:intergenic
ACAACCTTCTTGTTGATTTG+AGG 0.472466 8_2:-13535278 None:intergenic
TTTGATGAATTTGGAGACTT+CGG 0.478525 8_2:+13535920 Msa1235500:three_prime_UTR
CTCAATCGAATTGGATGCTA+AGG 0.488315 8_2:-13535687 None:intergenic
ATATCACCGAAATCAAAATC+AGG 0.494934 8_2:-13535998 None:intergenic
TCAGATACATCAGTGGCATC+AGG 0.497181 8_2:-13535098 None:intergenic
AGAGTGGGATCAGTCCCAAT+TGG 0.498263 8_2:-13535845 None:intergenic
TTCCACTGAGAATGAGGAGG+AGG 0.498809 8_2:-13533920 None:intergenic
GCAGCAGCCTCGGTGTTTGA+TGG 0.499088 8_2:-13535722 None:intergenic
TCTCCAAATTCATCAAAATC+CGG 0.501543 8_2:-13535914 None:intergenic
CACTGAGAATGAGGAGGAGG+AGG 0.502790 8_2:-13533917 None:intergenic
TCAATTTGGATTCTTTCAAA+GGG 0.504678 8_2:-13535043 None:intergenic
AATACCGCGGTGTTCGTATG+AGG 0.504954 8_2:+13535345 Msa1235500:three_prime_UTR
TATGAGGCCATGGGGCAAAT+GGG 0.507279 8_2:+13535361 Msa1235500:three_prime_UTR
CGGTGTTCGTATGAGGCCAT+GGG 0.508951 8_2:+13535352 Msa1235500:three_prime_UTR
TTGGATTCTTTCAAAGGGTT+AGG 0.509411 8_2:-13535038 None:intergenic
CCCTGTGGTTGGAGAAGTTG+AGG 0.509822 8_2:-13535306 None:intergenic
TCCAACTCAAGCACGGAAGA+AGG 0.513314 8_2:-13535662 None:intergenic
TCGAGGGGCTGCTGCAGAAG+CGG 0.520027 8_2:-13535528 None:intergenic
ACCTCAACTTCTCCAACCAC+AGG 0.522334 8_2:+13535305 Msa1235500:three_prime_UTR
CACAAGGTATGTTGTGTTGG+CGG 0.523962 8_2:-13536060 None:intergenic
TATTCTGGAATCAGTTGTTG+GGG 0.535730 8_2:+13534466 Msa1235500:CDS
TGTTCAATGCAGCAAGTCGA+GGG 0.537113 8_2:-13535544 None:intergenic
TGAATTTGCTGGTTGGATTG+AGG 0.543020 8_2:+13536033 Msa1235500:three_prime_UTR
ATGATGATGAATTTGCTGGT+TGG 0.546189 8_2:+13536026 Msa1235500:three_prime_UTR
TCAAAATCAGGAAGCTCAGT+AGG 0.547403 8_2:-13535986 None:intergenic
AGACACAAGGTATGTTGTGT+TGG 0.548226 8_2:-13536063 None:intergenic
GATTATAGAAAATGAATGAA+AGG 0.548978 8_2:-13534887 None:intergenic
TTGTTCAATGCAGCAAGTCG+AGG 0.554361 8_2:-13535545 None:intergenic
TCAGCAGCCCATTTGCCCCA+TGG 0.555170 8_2:-13535368 None:intergenic
AGTAAAGTTCAGGAACCCTT+CGG 0.555444 8_2:-13536321 None:intergenic
TGTCAATGAAGCGAGTGAAA+TGG 0.557538 8_2:+13535745 Msa1235500:three_prime_UTR
CAAGGTATGTTGTGTTGGCG+GGG 0.560891 8_2:-13536058 None:intergenic
CAAGATCGTCTCCCTGTGGT+TGG 0.561188 8_2:-13535317 None:intergenic
TAACATTGACGATTTCGGAC+CGG 0.572404 8_2:+13535895 Msa1235500:three_prime_UTR
TGCTGTTAGAGTCGTCCGAA+GGG 0.573780 8_2:+13536306 Msa1235500:three_prime_UTR
ACTTAAATTTCAGGCAATGG+AGG 0.581959 8_2:+13535490 Msa1235500:three_prime_UTR
ACTGCTGCAGGTACATGAGG+TGG 0.583732 8_2:-13535179 None:intergenic
TTGTTGCTGAGAATCCAATT+GGG 0.585213 8_2:+13535831 Msa1235500:three_prime_UTR
GTGGTTGGAGAAGTTGAGGT+TGG 0.588127 8_2:-13535302 None:intergenic
GAAGTCAACGAAACTGCTGC+AGG 0.590286 8_2:-13535191 None:intergenic
TTCTCAGCAACAATTGACTC+AGG 0.591804 8_2:-13535821 None:intergenic
AGCCTCCAAAACACACAACT+CGG 0.594430 8_2:-13534777 None:intergenic
CAGATTCTAACATTTCTGAA+AGG 0.601245 8_2:-13535401 None:intergenic
ATACAGTGAGAGATAGTGAC+AGG 0.601697 8_2:+13533849 Msa1235500:five_prime_UTR
CTGCTGCAGGTACATGAGGT+GGG 0.605078 8_2:-13535178 None:intergenic
TGAAGCCTAAGACTGTATCA+AGG 0.608688 8_2:+13535231 Msa1235500:three_prime_UTR
TCAAATCCAAAATCAAGAGT+GGG 0.608872 8_2:-13535860 None:intergenic
AAGAATCCAAATTGATGACA+AGG 0.614973 8_2:+13535051 Msa1235500:three_prime_UTR
AGTCTCTTAGGAGTGAGACT+AGG 0.623010 8_2:+13536095 Msa1235500:three_prime_UTR
ACAACCTCAAATCAACAAGA+AGG 0.623642 8_2:+13535274 Msa1235500:three_prime_UTR
GTTCAATGCAGCAAGTCGAG+GGG 0.629744 8_2:-13535543 None:intergenic
GAAAATCTAGAGCTAGACAA+AGG 0.632966 8_2:+13535015 Msa1235500:three_prime_UTR
GACTACAAAACTTAGACACA+AGG 0.640936 8_2:-13536076 None:intergenic
GAAACTGCTGCAGGTACATG+AGG 0.646090 8_2:-13535182 None:intergenic
TAAGGTATCCAACTCAAGCA+CGG 0.649733 8_2:-13535669 None:intergenic
GGCACAAGATCGTCTCCCTG+TGG 0.652150 8_2:-13535321 None:intergenic
CCTCAACTTCTCCAACCACA+GGG 0.653648 8_2:+13535306 Msa1235500:three_prime_UTR
CTCCTCCTCCTCATTCTCAG+TGG 0.680005 8_2:+13533918 Msa1235500:five_prime_UTR
AAGAAACAGAGAAAATCCAG+AGG 0.688778 8_2:-13534502 None:intergenic
CTCGTCTTCAGATACATCAG+TGG 0.692327 8_2:-13535105 None:intergenic
TTTGAAGGTTCATTCATACG+CGG 0.699500 8_2:-13536381 None:intergenic
CGATCTTGTGCCAAATACCG+CGG 0.713089 8_2:+13535332 Msa1235500:three_prime_UTR
GGTGTTCGTATGAGGCCATG+GGG 0.719703 8_2:+13535353 Msa1235500:three_prime_UTR
TGATGTTCCATCAAACACCG+AGG 0.748525 8_2:+13535715 Msa1235500:three_prime_UTR
ATGGCCTCATACGAACACCG+CGG 0.828126 8_2:-13535349 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATTGAAAAAAATTCT+TGG + chr8_2:13534080-13534099 Msa1235500:intron 10.0%
!! AAAACAATAAAAAATTGTTT+GGG - chr8_2:13534974-13534993 None:intergenic 10.0%
!!! TAATAAAAAACAAACCTTTT+AGG - chr8_2:13534611-13534630 None:intergenic 15.0%
!! CAAAACAATAAAAAATTGTT+TGG - chr8_2:13534975-13534994 None:intergenic 15.0%
!! AAAAAAACAACCAAATCAAA+GGG + chr8_2:13533941-13533960 Msa1235500:five_prime_UTR 20.0%
!!! GAAAAAATGAGTTTTTTCTT+GGG - chr8_2:13534005-13534024 None:intergenic 20.0%
!!! TGAAAAAATGAGTTTTTTCT+TGG - chr8_2:13534006-13534025 None:intergenic 20.0%
!!! GTGTTATTATTTTATTGATC+TGG - chr8_2:13534246-13534265 None:intergenic 20.0%
!! GATTATAGAAAATGAATGAA+AGG - chr8_2:13534890-13534909 None:intergenic 20.0%
!!! CTCACAAAATTTTTAGAAAA+TGG - chr8_2:13534922-13534941 None:intergenic 20.0%
!!! CATTTTCTAAAAATTTTGTG+AGG + chr8_2:13534920-13534939 Msa1235500:three_prime_UTR 20.0%
!!! TCATTTTGAACTTAAATTTC+AGG + chr8_2:13535481-13535500 Msa1235500:three_prime_UTR 20.0%
!!! ATTTTGGATTTGATTTTGAT+CGG + chr8_2:13535870-13535889 Msa1235500:three_prime_UTR 20.0%
! GAAAAAAACAACCAAATCAA+AGG + chr8_2:13533940-13533959 Msa1235500:five_prime_UTR 25.0%
!! AAGAATTTTACCCTTTGATT+TGG - chr8_2:13533954-13533973 None:intergenic 25.0%
! TTGAAAAAAATTCTTGGTCT+TGG + chr8_2:13534086-13534105 Msa1235500:intron 25.0%
! GATGATTATTGTTTCATAGA+TGG + chr8_2:13534694-13534713 Msa1235500:intron 25.0%
!!! TCTTTTTTTGTTCTTAAGAG+TGG + chr8_2:13534822-13534841 Msa1235500:CDS 25.0%
! AAATGAATGAAAGGCAAAAA+TGG - chr8_2:13534881-13534900 None:intergenic 25.0%
!!! ATTGTTTTGATTCAACATGT+CGG + chr8_2:13534986-13535005 Msa1235500:three_prime_UTR 25.0%
! TCAATTTGGATTCTTTCAAA+GGG - chr8_2:13535046-13535065 None:intergenic 25.0%
! ATCAATTTGGATTCTTTCAA+AGG - chr8_2:13535047-13535066 None:intergenic 25.0%
!! AAGTTTAAAACAGTCATATC+TGG - chr8_2:13535800-13535819 None:intergenic 25.0%
! TTCAAGGATATTCAAATTCA+TGG + chr8_2:13535947-13535966 Msa1235500:three_prime_UTR 25.0%
! CTGAACTTTACTATATTGTT+CGG + chr8_2:13536332-13536351 Msa1235500:three_prime_UTR 25.0%
!! ATTGATCTGGAATTTTGTGT+TGG - chr8_2:13534233-13534252 None:intergenic 30.0%
! CACACTCATGTGATTTTATT+TGG + chr8_2:13534263-13534282 Msa1235500:intron 30.0%
GATAAAGGATCACAAATCTA+CGG - chr8_2:13534313-13534332 None:intergenic 30.0%
ACACACGCTAATATAGATAA+AGG - chr8_2:13534328-13534347 None:intergenic 30.0%
! ACTCTTTTTCTTCTTCTGTT+TGG + chr8_2:13534364-13534383 Msa1235500:intron 30.0%
! AATTTGCTCTCAAAAACAAG+AGG + chr8_2:13534417-13534436 Msa1235500:five_prime_UTR 30.0%
TATGTCTTCTCAGTTTATTC+TGG + chr8_2:13534451-13534470 Msa1235500:exon 30.0%
!! TTTATTCTGGAATCAGTTGT+TGG + chr8_2:13534464-13534483 Msa1235500:CDS 30.0%
!! TTATTCTGGAATCAGTTGTT+GGG + chr8_2:13534465-13534484 Msa1235500:CDS 30.0%
! ATTGTTTCATAGATGGATTC+TGG + chr8_2:13534701-13534720 Msa1235500:intron 30.0%
! TTGTTTCATAGATGGATTCT+GGG + chr8_2:13534702-13534721 Msa1235500:intron 30.0%
!!! TTTGTGTCTGTCATTTTTTC+AGG + chr8_2:13534746-13534765 Msa1235500:intron 30.0%
! AAATTTTGTGAGGATTCTGA+AGG + chr8_2:13534930-13534949 Msa1235500:three_prime_UTR 30.0%
! AATTTTGTGAGGATTCTGAA+GGG + chr8_2:13534931-13534950 Msa1235500:three_prime_UTR 30.0%
!!! TTGTTTTGATTCAACATGTC+GGG + chr8_2:13534987-13535006 Msa1235500:three_prime_UTR 30.0%
! AAGAATCCAAATTGATGACA+AGG + chr8_2:13535051-13535070 Msa1235500:three_prime_UTR 30.0%
CAATTTCCTTGTCATCAATT+TGG - chr8_2:13535060-13535079 None:intergenic 30.0%
AACAAAAAGAAGCTTCTTTG+AGG + chr8_2:13535148-13535167 Msa1235500:three_prime_UTR 30.0%
CAGATTCTAACATTTCTGAA+AGG - chr8_2:13535404-13535423 None:intergenic 30.0%
CTTTCAGAAATGTTAGAATC+TGG + chr8_2:13535402-13535421 Msa1235500:three_prime_UTR 30.0%
AGAAATGTTAGAATCTGGTT+GGG + chr8_2:13535407-13535426 Msa1235500:three_prime_UTR 30.0%
CTCAAGCTTATGAATCTAAA+AGG + chr8_2:13535456-13535475 Msa1235500:three_prime_UTR 30.0%
TGAACTTAAATTTCAGGCAA+TGG + chr8_2:13535487-13535506 Msa1235500:three_prime_UTR 30.0%
ATCAAACTTCTCAATCGAAT+TGG - chr8_2:13535699-13535718 None:intergenic 30.0%
TCAAATCCAAAATCAAGAGT+GGG - chr8_2:13535863-13535882 None:intergenic 30.0%
ATCAAATCCAAAATCAAGAG+TGG - chr8_2:13535864-13535883 None:intergenic 30.0%
TCTCCAAATTCATCAAAATC+CGG - chr8_2:13535917-13535936 None:intergenic 30.0%
TTTGATGAATTTGGAGACTT+CGG + chr8_2:13535920-13535939 Msa1235500:three_prime_UTR 30.0%
ATATCACCGAAATCAAAATC+AGG - chr8_2:13536001-13536020 None:intergenic 30.0%
!!! GATTTTGATTTCGGTGATAT+TGG + chr8_2:13536001-13536020 Msa1235500:three_prime_UTR 30.0%
!! TCTAAGTTTTGTAGTCTCTT+AGG + chr8_2:13536083-13536102 Msa1235500:three_prime_UTR 30.0%
! GGATATACAATATCCGTTTT+TGG + chr8_2:13536187-13536206 Msa1235500:three_prime_UTR 30.0%
CGAACAATATAGTAAAGTTC+AGG - chr8_2:13536334-13536353 None:intergenic 30.0%
TGAGAGAAAGAGAAAAACAG+AGG + chr8_2:13533880-13533899 Msa1235500:five_prime_UTR 35.0%
!!! GTTTTTTTCCACTGAGAATG+AGG - chr8_2:13533929-13533948 None:intergenic 35.0%
TTTGTCTGTGTGTCATACTA+AGG + chr8_2:13534145-13534164 Msa1235500:intron 35.0%
!! TCTTCTTCTGTTTGGTTTGA+AGG + chr8_2:13534372-13534391 Msa1235500:intron 35.0%
!! AAGGTTGAAGTGTTTGTTGT+TGG + chr8_2:13534391-13534410 Msa1235500:intron 35.0%
!! TATTCTGGAATCAGTTGTTG+GGG + chr8_2:13534466-13534485 Msa1235500:CDS 35.0%
AAGAAACAGAGAAAATCCAG+AGG - chr8_2:13534505-13534524 None:intergenic 35.0%
!! GCAATTTTTCAATGCGATTG+AGG + chr8_2:13534585-13534604 Msa1235500:CDS 35.0%
! ATAGATGGATTCTGGGTTTA+AGG + chr8_2:13534709-13534728 Msa1235500:intron 35.0%
! TAGATGGATTCTGGGTTTAA+GGG + chr8_2:13534710-13534729 Msa1235500:intron 35.0%
!! ATTTTGTGAGGATTCTGAAG+GGG + chr8_2:13534932-13534951 Msa1235500:three_prime_UTR 35.0%
!! ATTCTGAAGGGGTTTTTCAA+GGG + chr8_2:13534943-13534962 Msa1235500:three_prime_UTR 35.0%
! GAAAATCTAGAGCTAGACAA+AGG + chr8_2:13535015-13535034 Msa1235500:three_prime_UTR 35.0%
TTGGATTCTTTCAAAGGGTT+AGG - chr8_2:13535041-13535060 None:intergenic 35.0%
TTTCTCCTTGATACAGTCTT+AGG - chr8_2:13535239-13535258 None:intergenic 35.0%
ACAACCTCAAATCAACAAGA+AGG + chr8_2:13535274-13535293 Msa1235500:three_prime_UTR 35.0%
!! ACAACCTTCTTGTTGATTTG+AGG - chr8_2:13535281-13535300 None:intergenic 35.0%
CAGAAATGTTAGAATCTGGT+TGG + chr8_2:13535406-13535425 Msa1235500:three_prime_UTR 35.0%
ACTTAAATTTCAGGCAATGG+AGG + chr8_2:13535490-13535509 Msa1235500:three_prime_UTR 35.0%
ATTGTTGCTGAGAATCCAAT+TGG + chr8_2:13535830-13535849 Msa1235500:three_prime_UTR 35.0%
TTGTTGCTGAGAATCCAATT+GGG + chr8_2:13535831-13535850 Msa1235500:three_prime_UTR 35.0%
CGGTTTAACATTGACGATTT+CGG + chr8_2:13535890-13535909 Msa1235500:three_prime_UTR 35.0%
!!! GAGCTTCCTGATTTTGATTT+CGG + chr8_2:13535992-13536011 Msa1235500:three_prime_UTR 35.0%
!! ATGATGATGAATTTGCTGGT+TGG + chr8_2:13536026-13536045 Msa1235500:three_prime_UTR 35.0%
GACTACAAAACTTAGACACA+AGG - chr8_2:13536079-13536098 None:intergenic 35.0%
!! TAGTTTTGCGAGACAATATG+AGG + chr8_2:13536166-13536185 Msa1235500:three_prime_UTR 35.0%
GAATCACTCTCAACCAAAAA+CGG - chr8_2:13536203-13536222 None:intergenic 35.0%
TTTGAAGGTTCATTCATACG+CGG - chr8_2:13536384-13536403 None:intergenic 35.0%
! TGTTTTTCTCACACTCTGAC+TGG - chr8_2:13533821-13533840 None:intergenic 40.0%
ATACAGTGAGAGATAGTGAC+AGG + chr8_2:13533849-13533868 Msa1235500:five_prime_UTR 40.0%
! TTTTTCCACTGAGAATGAGG+AGG - chr8_2:13533926-13533945 None:intergenic 40.0%
TCTGTGTGTCATACTAAGGT+CGG + chr8_2:13534149-13534168 Msa1235500:intron 40.0%
!! GTTGAAGTGTTTGTTGTTGG+AGG + chr8_2:13534394-13534413 Msa1235500:five_prime_UTR 40.0%
! TGTTACCGAGTTGTGTGTTT+TGG + chr8_2:13534772-13534791 Msa1235500:CDS 40.0%
!! GATTCTGAAGGGGTTTTTCA+AGG + chr8_2:13534942-13534961 Msa1235500:three_prime_UTR 40.0%
TGAAGCCTAAGACTGTATCA+AGG + chr8_2:13535231-13535250 Msa1235500:three_prime_UTR 40.0%
GCAATGGAGGCAATCAAAAA+GGG + chr8_2:13535503-13535522 Msa1235500:three_prime_UTR 40.0%
! TAAGGTATCCAACTCAAGCA+CGG - chr8_2:13535672-13535691 None:intergenic 40.0%
CTCAATCGAATTGGATGCTA+AGG - chr8_2:13535690-13535709 None:intergenic 40.0%
TGTCAATGAAGCGAGTGAAA+TGG + chr8_2:13535745-13535764 Msa1235500:three_prime_UTR 40.0%
! TTCTCAGCAACAATTGACTC+AGG - chr8_2:13535824-13535843 None:intergenic 40.0%
! ACTGATCCCACTCTTGATTT+TGG + chr8_2:13535854-13535873 Msa1235500:three_prime_UTR 40.0%
TAACATTGACGATTTCGGAC+CGG + chr8_2:13535895-13535914 Msa1235500:three_prime_UTR 40.0%
!! GGACCGGATTTTGATGAATT+TGG + chr8_2:13535911-13535930 Msa1235500:three_prime_UTR 40.0%
TCAAAATCAGGAAGCTCAGT+AGG - chr8_2:13535989-13536008 None:intergenic 40.0%
! GGTGATGATGATGAATTTGC+TGG + chr8_2:13536022-13536041 Msa1235500:three_prime_UTR 40.0%
!! TGAATTTGCTGGTTGGATTG+AGG + chr8_2:13536033-13536052 Msa1235500:three_prime_UTR 40.0%
AGACACAAGGTATGTTGTGT+TGG - chr8_2:13536066-13536085 None:intergenic 40.0%
AGTAAAGTTCAGGAACCCTT+CGG - chr8_2:13536324-13536343 None:intergenic 40.0%
CAATGCGATTGAGGCCTAAA+AGG + chr8_2:13534594-13534613 Msa1235500:CDS 45.0%
!! TACCGAGTTGTGTGTTTTGG+AGG + chr8_2:13534775-13534794 Msa1235500:CDS 45.0%
AGCCTCCAAAACACACAACT+CGG - chr8_2:13534780-13534799 None:intergenic 45.0%
TCAGATACATCAGTGGCATC+AGG - chr8_2:13535101-13535120 None:intergenic 45.0%
CTCGTCTTCAGATACATCAG+TGG - chr8_2:13535108-13535127 None:intergenic 45.0%
GGCAATGGAGGCAATCAAAA+AGG + chr8_2:13535502-13535521 Msa1235500:three_prime_UTR 45.0%
TGTTCAATGCAGCAAGTCGA+GGG - chr8_2:13535547-13535566 None:intergenic 45.0%
TTGTTCAATGCAGCAAGTCG+AGG - chr8_2:13535548-13535567 None:intergenic 45.0%
TGATGTTCCATCAAACACCG+AGG + chr8_2:13535715-13535734 Msa1235500:three_prime_UTR 45.0%
! ACAAGGTATGTTGTGTTGGC+GGG - chr8_2:13536062-13536081 None:intergenic 45.0%
CACAAGGTATGTTGTGTTGG+CGG - chr8_2:13536063-13536082 None:intergenic 45.0%
AGTCTCTTAGGAGTGAGACT+AGG + chr8_2:13536095-13536114 Msa1235500:three_prime_UTR 45.0%
TTCCACTGAGAATGAGGAGG+AGG - chr8_2:13533923-13533942 None:intergenic 50.0%
GAAACTGCTGCAGGTACATG+AGG - chr8_2:13535185-13535204 None:intergenic 50.0%
GAAGTCAACGAAACTGCTGC+AGG - chr8_2:13535194-13535213 None:intergenic 50.0%
GTGGTTGGAGAAGTTGAGGT+TGG - chr8_2:13535305-13535324 None:intergenic 50.0%
ACCTCAACTTCTCCAACCAC+AGG + chr8_2:13535305-13535324 Msa1235500:three_prime_UTR 50.0%
CCTCAACTTCTCCAACCACA+GGG + chr8_2:13535306-13535325 Msa1235500:three_prime_UTR 50.0%
CGATCTTGTGCCAAATACCG+CGG + chr8_2:13535332-13535351 Msa1235500:three_prime_UTR 50.0%
CATACGAACACCGCGGTATT+TGG - chr8_2:13535345-13535364 None:intergenic 50.0%
AATACCGCGGTGTTCGTATG+AGG + chr8_2:13535345-13535364 Msa1235500:three_prime_UTR 50.0%
TATGAGGCCATGGGGCAAAT+GGG + chr8_2:13535361-13535380 Msa1235500:three_prime_UTR 50.0%
GTTCAATGCAGCAAGTCGAG+GGG - chr8_2:13535546-13535565 None:intergenic 50.0%
TCCAACTCAAGCACGGAAGA+AGG - chr8_2:13535665-13535684 None:intergenic 50.0%
TTCATTGACAGCAGCAGCCT+CGG - chr8_2:13535735-13535754 None:intergenic 50.0%
AGAGTGGGATCAGTCCCAAT+TGG - chr8_2:13535848-13535867 None:intergenic 50.0%
TGGAGACTTCGGTGACTTCA+AGG + chr8_2:13535931-13535950 Msa1235500:three_prime_UTR 50.0%
! CAAGGTATGTTGTGTTGGCG+GGG - chr8_2:13536061-13536080 None:intergenic 50.0%
TGCTGTTAGAGTCGTCCGAA+GGG + chr8_2:13536306-13536325 Msa1235500:three_prime_UTR 50.0%
CACTGAGAATGAGGAGGAGG+AGG - chr8_2:13533920-13533939 None:intergenic 55.0%
CTCCTCCTCCTCATTCTCAG+TGG + chr8_2:13533918-13533937 Msa1235500:five_prime_UTR 55.0%
GGGCTCTGTTGAAGAACCTC+TGG + chr8_2:13534486-13534505 Msa1235500:CDS 55.0%
CTGCTGCAGGTACATGAGGT+GGG - chr8_2:13535181-13535200 None:intergenic 55.0%
ACTGCTGCAGGTACATGAGG+TGG - chr8_2:13535182-13535201 None:intergenic 55.0%
CCCTGTGGTTGGAGAAGTTG+AGG - chr8_2:13535309-13535328 None:intergenic 55.0%
CAAGATCGTCTCCCTGTGGT+TGG - chr8_2:13535320-13535339 None:intergenic 55.0%
ATGGCCTCATACGAACACCG+CGG - chr8_2:13535352-13535371 None:intergenic 55.0%
CGGTGTTCGTATGAGGCCAT+GGG + chr8_2:13535352-13535371 Msa1235500:three_prime_UTR 55.0%
GGTGTTCGTATGAGGCCATG+GGG + chr8_2:13535353-13535372 Msa1235500:three_prime_UTR 55.0%
GTATGAGGCCATGGGGCAAA+TGG + chr8_2:13535360-13535379 Msa1235500:three_prime_UTR 55.0%
GCCTTCTTCCGTGCTTGAGT+TGG + chr8_2:13535661-13535680 Msa1235500:three_prime_UTR 55.0%
! GTGCTGTTAGAGTCGTCCGA+AGG + chr8_2:13536305-13536324 Msa1235500:three_prime_UTR 55.0%
GGCACAAGATCGTCTCCCTG+TGG - chr8_2:13535324-13535343 None:intergenic 60.0%
GCGGTGTTCGTATGAGGCCA+TGG + chr8_2:13535351-13535370 Msa1235500:three_prime_UTR 60.0%
TCAGCAGCCCATTTGCCCCA+TGG - chr8_2:13535371-13535390 None:intergenic 60.0%
!! GCAGCAGCCTCGGTGTTTGA+TGG - chr8_2:13535725-13535744 None:intergenic 60.0%
TCGAGGGGCTGCTGCAGAAG+CGG - chr8_2:13535531-13535550 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 13533814 13536409 13533814 ID=Msa1235500;Name=Msa1235500
chr8_2 mRNA 13533814 13536409 13533814 ID=Msa1235500-mRNA-1;Parent=Msa1235500;Name=Msa1235500-mRNA-1;_AED=0.27;_eAED=0.27;_QI=207|1|1|1|0|0|3|1554|83
chr8_2 exon 13533814 13533962 13533814 ID=Msa1235500-mRNA-1:exon:5202;Parent=Msa1235500-mRNA-1
chr8_2 exon 13534394 13534615 13534394 ID=Msa1235500-mRNA-1:exon:5203;Parent=Msa1235500-mRNA-1
chr8_2 exon 13534768 13536409 13534768 ID=Msa1235500-mRNA-1:exon:5204;Parent=Msa1235500-mRNA-1
chr8_2 five_prime_UTR 13533814 13533962 13533814 ID=Msa1235500-mRNA-1:five_prime_utr;Parent=Msa1235500-mRNA-1
chr8_2 five_prime_UTR 13534394 13534451 13534394 ID=Msa1235500-mRNA-1:five_prime_utr;Parent=Msa1235500-mRNA-1
chr8_2 CDS 13534452 13534615 13534452 ID=Msa1235500-mRNA-1:cds;Parent=Msa1235500-mRNA-1
chr8_2 CDS 13534768 13534855 13534768 ID=Msa1235500-mRNA-1:cds;Parent=Msa1235500-mRNA-1
chr8_2 three_prime_UTR 13534856 13536409 13534856 ID=Msa1235500-mRNA-1:three_prime_utr;Parent=Msa1235500-mRNA-1
Gene Sequence

>Msa1235500

ATGTCTTCTCAGTTTATTCTGGAATCAGTTGTTGGGGCTCTGTTGAAGAACCTCTGGATTTTCTCTGTTTCTTGTCTTCTGCTCACTCTTTCTGTGTTCTGTTCTGCTCTCTATATGCTCTTTGTCATCAAGAGCAATTTTTCAATGCGATTGAGGCCTAAAAGGACTTGTTACCGAGTTGTGTGTTTTGGAGGCTTTCATATACAGAAATTTTCTAATCTTTTTTTGTTCTTAAGAGTGGATCTCACTTGA
Protein sequence

>Msa1235500

MSSQFILESVVGALLKNLWIFSVSCLLLTLSVFCSALYMLFVIKSNFSMRLRPKRTCYRVVCFGGFHIQKFSNLFLFLRVDLT*