AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1315780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1315780 MtrunA17_Chr8g0345521 87.640 89 5 1 1 83 1 89 3.47e-48 147
Msa1315780 MtrunA17_Chr8g0345541 83.133 83 14 0 1 83 1 83 1.17e-42 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 101 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATGAACCTTCAAATATTT+TGG 0.096347 8_4:+15337674 Msa1315780:three_prime_UTR
ACTGATCCCACTCTTGATTT+TGG 0.147095 8_4:+15337139 Msa1315780:three_prime_UTR
CAATTTCCTTGTCATCAATT+TGG 0.159156 8_4:-15336342 None:intergenic
CATACGAACACCGCGGTATT+TGG 0.219306 8_4:-15336627 None:intergenic
TATGTCTTCTCAGTTTATTC+TGG 0.229280 8_4:+15335736 Msa1315780:exon
CGGTTTAACATTGACGATTT+CGG 0.262003 8_4:+15337175 Msa1315780:three_prime_UTR
GGCAATGGAGGCAATCAAAA+AGG 0.264426 8_4:+15336787 Msa1315780:three_prime_UTR
CTCAAGCTTATGAATCTAAA+AGG 0.275287 8_4:+15336741 Msa1315780:three_prime_UTR
CAATGCGATTGAGGCCTAAA+AGG 0.279868 8_4:+15335879 Msa1315780:CDS
TTTCTCCTTGATACAGTCTT+AGG 0.281725 8_4:-15336521 None:intergenic
TTCAAGGATATTCAAATTCA+TGG 0.318937 8_4:+15337232 Msa1315780:three_prime_UTR
TGTTACCGAGTTGTGTGTTT+TGG 0.333384 8_4:+15336057 Msa1315780:CDS
TTATTCTGGAATCAGTTGTT+GGG 0.348752 8_4:+15335750 Msa1315780:CDS
ATCAAACTTCTCAATCGAAT+TGG 0.353862 8_4:-15336981 None:intergenic
CTTTCAGAAATGTTAGAATC+TGG 0.376430 8_4:+15336687 Msa1315780:three_prime_UTR
TTTATTCTGGAATCAGTTGT+TGG 0.383668 8_4:+15335749 Msa1315780:CDS
ATCAATTTGGATTCTTTCAA+AGG 0.400070 8_4:-15336329 None:intergenic
GGGCTCTGTTGAAGAACCTC+TGG 0.401388 8_4:+15335771 Msa1315780:CDS
GGTGATGATGATGAATTTGC+TGG 0.406438 8_4:+15337307 Msa1315780:three_prime_UTR
ATTGTTGCTGAGAATCCAAT+TGG 0.407295 8_4:+15337115 Msa1315780:three_prime_UTR
AAGGTTGAAGTGTTTGTTGT+TGG 0.411506 8_4:+15335676 Msa1315780:intron
AAGTTTAAAACAGTCATATC+TGG 0.411631 8_4:-15337082 None:intergenic
AATGTATCCAAAATATTTGA+AGG 0.416141 8_4:-15337681 None:intergenic
TGGAGACTTCGGTGACTTCA+AGG 0.419980 8_4:+15337216 Msa1315780:three_prime_UTR
GCCTTCTTCCGTGCTTGAGT+TGG 0.420542 8_4:+15336946 Msa1315780:three_prime_UTR
CTGAACTTTACTATATTGTT+CGG 0.427450 8_4:+15337617 Msa1315780:three_prime_UTR
GTATGAGGCCATGGGGCAAA+TGG 0.449753 8_4:+15336645 Msa1315780:three_prime_UTR
CGAACAATATAGTAAAGTTC+AGG 0.452336 8_4:-15337616 None:intergenic
GTTGAAGTGTTTGTTGTTGG+AGG 0.456195 8_4:+15335679 Msa1315780:five_prime_UTR
ACAAGGTATGTTGTGTTGGC+GGG 0.457585 8_4:-15337344 None:intergenic
CAGAAATGTTAGAATCTGGT+TGG 0.458650 8_4:+15336691 Msa1315780:three_prime_UTR
AGAAATGTTAGAATCTGGTT+GGG 0.458838 8_4:+15336692 Msa1315780:three_prime_UTR
TGAACTTAAATTTCAGGCAA+TGG 0.461033 8_4:+15336772 Msa1315780:three_prime_UTR
GCGGTGTTCGTATGAGGCCA+TGG 0.462248 8_4:+15336636 Msa1315780:three_prime_UTR
TTCATTGACAGCAGCAGCCT+CGG 0.463601 8_4:-15337017 None:intergenic
ATCAAATCCAAAATCAAGAG+TGG 0.471193 8_4:-15337146 None:intergenic
ACAACCTTCTTGTTGATTTG+AGG 0.472466 8_4:-15336563 None:intergenic
TTTGATGAATTTGGAGACTT+CGG 0.478525 8_4:+15337205 Msa1315780:three_prime_UTR
CTCAATCGAATTGGATGCTA+AGG 0.488315 8_4:-15336972 None:intergenic
ATATCACCGAAATCAAAATC+AGG 0.494934 8_4:-15337283 None:intergenic
TCAGATACATCAGTGGCATC+AGG 0.497181 8_4:-15336383 None:intergenic
AGAGTGGGATCAGTCCCAAT+TGG 0.498263 8_4:-15337130 None:intergenic
TTCCACTGAGAATGAGGAGG+AGG 0.498809 8_4:-15335205 None:intergenic
GCAGCAGCCTCGGTGTTTGA+TGG 0.499088 8_4:-15337007 None:intergenic
TCTCCAAATTCATCAAAATC+CGG 0.501543 8_4:-15337199 None:intergenic
CACTGAGAATGAGGAGGAGG+AGG 0.502790 8_4:-15335202 None:intergenic
TCAATTTGGATTCTTTCAAA+GGG 0.504678 8_4:-15336328 None:intergenic
AATACCGCGGTGTTCGTATG+AGG 0.504954 8_4:+15336630 Msa1315780:three_prime_UTR
TATGAGGCCATGGGGCAAAT+GGG 0.507279 8_4:+15336646 Msa1315780:three_prime_UTR
CGGTGTTCGTATGAGGCCAT+GGG 0.508951 8_4:+15336637 Msa1315780:three_prime_UTR
TTGGATTCTTTCAAAGGGTT+AGG 0.509411 8_4:-15336323 None:intergenic
CCCTGTGGTTGGAGAAGTTG+AGG 0.509822 8_4:-15336591 None:intergenic
TCCAACTCAAGCACGGAAGA+AGG 0.513314 8_4:-15336947 None:intergenic
TCGAGGGGCTGCTGCAGAAG+CGG 0.520027 8_4:-15336813 None:intergenic
ACCTCAACTTCTCCAACCAC+AGG 0.522334 8_4:+15336590 Msa1315780:three_prime_UTR
CACAAGGTATGTTGTGTTGG+CGG 0.523962 8_4:-15337345 None:intergenic
TATTCTGGAATCAGTTGTTG+GGG 0.535730 8_4:+15335751 Msa1315780:CDS
TGTTCAATGCAGCAAGTCGA+GGG 0.537113 8_4:-15336829 None:intergenic
TGAATTTGCTGGTTGGATTG+AGG 0.543020 8_4:+15337318 Msa1315780:three_prime_UTR
ATGATGATGAATTTGCTGGT+TGG 0.546189 8_4:+15337311 Msa1315780:three_prime_UTR
TCAAAATCAGGAAGCTCAGT+AGG 0.547403 8_4:-15337271 None:intergenic
AGACACAAGGTATGTTGTGT+TGG 0.548226 8_4:-15337348 None:intergenic
GATTATAGAAAATGAATGAA+AGG 0.548978 8_4:-15336172 None:intergenic
TTGTTCAATGCAGCAAGTCG+AGG 0.554361 8_4:-15336830 None:intergenic
TCAGCAGCCCATTTGCCCCA+TGG 0.555170 8_4:-15336653 None:intergenic
AGTAAAGTTCAGGAACCCTT+CGG 0.555444 8_4:-15337606 None:intergenic
TGTCAATGAAGCGAGTGAAA+TGG 0.557538 8_4:+15337030 Msa1315780:three_prime_UTR
CAAGGTATGTTGTGTTGGCG+GGG 0.560891 8_4:-15337343 None:intergenic
CAAGATCGTCTCCCTGTGGT+TGG 0.561188 8_4:-15336602 None:intergenic
TAACATTGACGATTTCGGAC+CGG 0.572404 8_4:+15337180 Msa1315780:three_prime_UTR
TGCTGTTAGAGTCGTCCGAA+GGG 0.573780 8_4:+15337591 Msa1315780:three_prime_UTR
ACTTAAATTTCAGGCAATGG+AGG 0.581959 8_4:+15336775 Msa1315780:three_prime_UTR
ACTGCTGCAGGTACATGAGG+TGG 0.583732 8_4:-15336464 None:intergenic
TTGTTGCTGAGAATCCAATT+GGG 0.585213 8_4:+15337116 Msa1315780:three_prime_UTR
GTGGTTGGAGAAGTTGAGGT+TGG 0.588127 8_4:-15336587 None:intergenic
GAAGTCAACGAAACTGCTGC+AGG 0.590286 8_4:-15336476 None:intergenic
TTCTCAGCAACAATTGACTC+AGG 0.591804 8_4:-15337106 None:intergenic
AGCCTCCAAAACACACAACT+CGG 0.594430 8_4:-15336062 None:intergenic
CAGATTCTAACATTTCTGAA+AGG 0.601245 8_4:-15336686 None:intergenic
ATACAGTGAGAGATAGTGAC+AGG 0.601697 8_4:+15335134 Msa1315780:five_prime_UTR
CTGCTGCAGGTACATGAGGT+GGG 0.605078 8_4:-15336463 None:intergenic
TGAAGCCTAAGACTGTATCA+AGG 0.608688 8_4:+15336516 Msa1315780:three_prime_UTR
TCAAATCCAAAATCAAGAGT+GGG 0.608872 8_4:-15337145 None:intergenic
AAGAATCCAAATTGATGACA+AGG 0.614973 8_4:+15336336 Msa1315780:three_prime_UTR
AGTCTCTTAGGAGTGAGACT+AGG 0.623010 8_4:+15337380 Msa1315780:three_prime_UTR
ACAACCTCAAATCAACAAGA+AGG 0.623642 8_4:+15336559 Msa1315780:three_prime_UTR
GTTCAATGCAGCAAGTCGAG+GGG 0.629744 8_4:-15336828 None:intergenic
GAAAATCTAGAGCTAGACAA+AGG 0.632966 8_4:+15336300 Msa1315780:three_prime_UTR
GACTACAAAACTTAGACACA+AGG 0.640936 8_4:-15337361 None:intergenic
GAAACTGCTGCAGGTACATG+AGG 0.646090 8_4:-15336467 None:intergenic
TAAGGTATCCAACTCAAGCA+CGG 0.649733 8_4:-15336954 None:intergenic
GGCACAAGATCGTCTCCCTG+TGG 0.652150 8_4:-15336606 None:intergenic
CCTCAACTTCTCCAACCACA+GGG 0.653648 8_4:+15336591 Msa1315780:three_prime_UTR
CTCCTCCTCCTCATTCTCAG+TGG 0.680005 8_4:+15335203 Msa1315780:five_prime_UTR
AAGAAACAGAGAAAATCCAG+AGG 0.688778 8_4:-15335787 None:intergenic
CTCGTCTTCAGATACATCAG+TGG 0.692327 8_4:-15336390 None:intergenic
TTTGAAGGTTCATTCATACG+CGG 0.699500 8_4:-15337666 None:intergenic
CGATCTTGTGCCAAATACCG+CGG 0.713089 8_4:+15336617 Msa1315780:three_prime_UTR
GGTGTTCGTATGAGGCCATG+GGG 0.719703 8_4:+15336638 Msa1315780:three_prime_UTR
TGATGTTCCATCAAACACCG+AGG 0.748525 8_4:+15337000 Msa1315780:three_prime_UTR
ATGGCCTCATACGAACACCG+CGG 0.828126 8_4:-15336634 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATTGAAAAAAATTCT+TGG + chr8_4:15335365-15335384 Msa1315780:intron 10.0%
!! AAAACAATAAAAAATTGTTT+GGG - chr8_4:15336259-15336278 None:intergenic 10.0%
!!! TAATAAAAAACAAACCTTTT+AGG - chr8_4:15335896-15335915 None:intergenic 15.0%
!! CAAAACAATAAAAAATTGTT+TGG - chr8_4:15336260-15336279 None:intergenic 15.0%
!! AAAAAAACAACCAAATCAAA+GGG + chr8_4:15335226-15335245 Msa1315780:five_prime_UTR 20.0%
!!! GAAAAAATGAGTTTTTTCTT+GGG - chr8_4:15335290-15335309 None:intergenic 20.0%
!!! TGAAAAAATGAGTTTTTTCT+TGG - chr8_4:15335291-15335310 None:intergenic 20.0%
!!! GTGTTATTATTTTATTGATC+TGG - chr8_4:15335531-15335550 None:intergenic 20.0%
!! GATTATAGAAAATGAATGAA+AGG - chr8_4:15336175-15336194 None:intergenic 20.0%
!!! CTCACAAAATTTTTAGAAAA+TGG - chr8_4:15336207-15336226 None:intergenic 20.0%
!!! CATTTTCTAAAAATTTTGTG+AGG + chr8_4:15336205-15336224 Msa1315780:three_prime_UTR 20.0%
!!! TCATTTTGAACTTAAATTTC+AGG + chr8_4:15336766-15336785 Msa1315780:three_prime_UTR 20.0%
!!! ATTTTGGATTTGATTTTGAT+CGG + chr8_4:15337155-15337174 Msa1315780:three_prime_UTR 20.0%
! GAAAAAAACAACCAAATCAA+AGG + chr8_4:15335225-15335244 Msa1315780:five_prime_UTR 25.0%
!! AAGAATTTTACCCTTTGATT+TGG - chr8_4:15335239-15335258 None:intergenic 25.0%
! TTGAAAAAAATTCTTGGTCT+TGG + chr8_4:15335371-15335390 Msa1315780:intron 25.0%
! GATGATTATTGTTTCATAGA+TGG + chr8_4:15335979-15335998 Msa1315780:intron 25.0%
!!! TCTTTTTTTGTTCTTAAGAG+TGG + chr8_4:15336107-15336126 Msa1315780:CDS 25.0%
! AAATGAATGAAAGGCAAAAA+TGG - chr8_4:15336166-15336185 None:intergenic 25.0%
!!! ATTGTTTTGATTCAACATGT+CGG + chr8_4:15336271-15336290 Msa1315780:three_prime_UTR 25.0%
! TCAATTTGGATTCTTTCAAA+GGG - chr8_4:15336331-15336350 None:intergenic 25.0%
! ATCAATTTGGATTCTTTCAA+AGG - chr8_4:15336332-15336351 None:intergenic 25.0%
!! AAGTTTAAAACAGTCATATC+TGG - chr8_4:15337085-15337104 None:intergenic 25.0%
! TTCAAGGATATTCAAATTCA+TGG + chr8_4:15337232-15337251 Msa1315780:three_prime_UTR 25.0%
! CTGAACTTTACTATATTGTT+CGG + chr8_4:15337617-15337636 Msa1315780:three_prime_UTR 25.0%
!! ATTGATCTGGAATTTTGTGT+TGG - chr8_4:15335518-15335537 None:intergenic 30.0%
! CACACTCATGTGATTTTATT+TGG + chr8_4:15335548-15335567 Msa1315780:intron 30.0%
GATAAAGGATCACAAATCTA+CGG - chr8_4:15335598-15335617 None:intergenic 30.0%
ACACACGCTAATATAGATAA+AGG - chr8_4:15335613-15335632 None:intergenic 30.0%
! ACTCTTTTTCTTCTTCTGTT+TGG + chr8_4:15335649-15335668 Msa1315780:intron 30.0%
! AATTTGCTCTCAAAAACAAG+AGG + chr8_4:15335702-15335721 Msa1315780:five_prime_UTR 30.0%
TATGTCTTCTCAGTTTATTC+TGG + chr8_4:15335736-15335755 Msa1315780:exon 30.0%
!! TTTATTCTGGAATCAGTTGT+TGG + chr8_4:15335749-15335768 Msa1315780:CDS 30.0%
!! TTATTCTGGAATCAGTTGTT+GGG + chr8_4:15335750-15335769 Msa1315780:CDS 30.0%
! ATTGTTTCATAGATGGATTC+TGG + chr8_4:15335986-15336005 Msa1315780:intron 30.0%
! TTGTTTCATAGATGGATTCT+GGG + chr8_4:15335987-15336006 Msa1315780:intron 30.0%
!!! TTTGTGTCTGTCATTTTTTC+AGG + chr8_4:15336031-15336050 Msa1315780:intron 30.0%
! AAATTTTGTGAGGATTCTGA+AGG + chr8_4:15336215-15336234 Msa1315780:three_prime_UTR 30.0%
! AATTTTGTGAGGATTCTGAA+GGG + chr8_4:15336216-15336235 Msa1315780:three_prime_UTR 30.0%
!!! TTGTTTTGATTCAACATGTC+GGG + chr8_4:15336272-15336291 Msa1315780:three_prime_UTR 30.0%
! AAGAATCCAAATTGATGACA+AGG + chr8_4:15336336-15336355 Msa1315780:three_prime_UTR 30.0%
CAATTTCCTTGTCATCAATT+TGG - chr8_4:15336345-15336364 None:intergenic 30.0%
AACAAAAAGAAGCTTCTTTG+AGG + chr8_4:15336433-15336452 Msa1315780:three_prime_UTR 30.0%
CAGATTCTAACATTTCTGAA+AGG - chr8_4:15336689-15336708 None:intergenic 30.0%
CTTTCAGAAATGTTAGAATC+TGG + chr8_4:15336687-15336706 Msa1315780:three_prime_UTR 30.0%
AGAAATGTTAGAATCTGGTT+GGG + chr8_4:15336692-15336711 Msa1315780:three_prime_UTR 30.0%
CTCAAGCTTATGAATCTAAA+AGG + chr8_4:15336741-15336760 Msa1315780:three_prime_UTR 30.0%
TGAACTTAAATTTCAGGCAA+TGG + chr8_4:15336772-15336791 Msa1315780:three_prime_UTR 30.0%
ATCAAACTTCTCAATCGAAT+TGG - chr8_4:15336984-15337003 None:intergenic 30.0%
TCAAATCCAAAATCAAGAGT+GGG - chr8_4:15337148-15337167 None:intergenic 30.0%
ATCAAATCCAAAATCAAGAG+TGG - chr8_4:15337149-15337168 None:intergenic 30.0%
TCTCCAAATTCATCAAAATC+CGG - chr8_4:15337202-15337221 None:intergenic 30.0%
TTTGATGAATTTGGAGACTT+CGG + chr8_4:15337205-15337224 Msa1315780:three_prime_UTR 30.0%
ATATCACCGAAATCAAAATC+AGG - chr8_4:15337286-15337305 None:intergenic 30.0%
!!! GATTTTGATTTCGGTGATAT+TGG + chr8_4:15337286-15337305 Msa1315780:three_prime_UTR 30.0%
!! TCTAAGTTTTGTAGTCTCTT+AGG + chr8_4:15337368-15337387 Msa1315780:three_prime_UTR 30.0%
! GGATATACAATATCCGTTTT+TGG + chr8_4:15337472-15337491 Msa1315780:three_prime_UTR 30.0%
CGAACAATATAGTAAAGTTC+AGG - chr8_4:15337619-15337638 None:intergenic 30.0%
TGAGAGAAAGAGAAAAACAG+AGG + chr8_4:15335165-15335184 Msa1315780:five_prime_UTR 35.0%
!!! GTTTTTTTCCACTGAGAATG+AGG - chr8_4:15335214-15335233 None:intergenic 35.0%
TTTGTCTGTGTGTCATACTA+AGG + chr8_4:15335430-15335449 Msa1315780:intron 35.0%
!! TCTTCTTCTGTTTGGTTTGA+AGG + chr8_4:15335657-15335676 Msa1315780:intron 35.0%
!! AAGGTTGAAGTGTTTGTTGT+TGG + chr8_4:15335676-15335695 Msa1315780:intron 35.0%
!! TATTCTGGAATCAGTTGTTG+GGG + chr8_4:15335751-15335770 Msa1315780:CDS 35.0%
AAGAAACAGAGAAAATCCAG+AGG - chr8_4:15335790-15335809 None:intergenic 35.0%
!! GCAATTTTTCAATGCGATTG+AGG + chr8_4:15335870-15335889 Msa1315780:CDS 35.0%
! ATAGATGGATTCTGGGTTTA+AGG + chr8_4:15335994-15336013 Msa1315780:intron 35.0%
! TAGATGGATTCTGGGTTTAA+GGG + chr8_4:15335995-15336014 Msa1315780:intron 35.0%
!! ATTTTGTGAGGATTCTGAAG+GGG + chr8_4:15336217-15336236 Msa1315780:three_prime_UTR 35.0%
!! ATTCTGAAGGGGTTTTTCAA+GGG + chr8_4:15336228-15336247 Msa1315780:three_prime_UTR 35.0%
! GAAAATCTAGAGCTAGACAA+AGG + chr8_4:15336300-15336319 Msa1315780:three_prime_UTR 35.0%
TTGGATTCTTTCAAAGGGTT+AGG - chr8_4:15336326-15336345 None:intergenic 35.0%
TTTCTCCTTGATACAGTCTT+AGG - chr8_4:15336524-15336543 None:intergenic 35.0%
ACAACCTCAAATCAACAAGA+AGG + chr8_4:15336559-15336578 Msa1315780:three_prime_UTR 35.0%
!! ACAACCTTCTTGTTGATTTG+AGG - chr8_4:15336566-15336585 None:intergenic 35.0%
CAGAAATGTTAGAATCTGGT+TGG + chr8_4:15336691-15336710 Msa1315780:three_prime_UTR 35.0%
ACTTAAATTTCAGGCAATGG+AGG + chr8_4:15336775-15336794 Msa1315780:three_prime_UTR 35.0%
ATTGTTGCTGAGAATCCAAT+TGG + chr8_4:15337115-15337134 Msa1315780:three_prime_UTR 35.0%
TTGTTGCTGAGAATCCAATT+GGG + chr8_4:15337116-15337135 Msa1315780:three_prime_UTR 35.0%
CGGTTTAACATTGACGATTT+CGG + chr8_4:15337175-15337194 Msa1315780:three_prime_UTR 35.0%
!!! GAGCTTCCTGATTTTGATTT+CGG + chr8_4:15337277-15337296 Msa1315780:three_prime_UTR 35.0%
!! ATGATGATGAATTTGCTGGT+TGG + chr8_4:15337311-15337330 Msa1315780:three_prime_UTR 35.0%
GACTACAAAACTTAGACACA+AGG - chr8_4:15337364-15337383 None:intergenic 35.0%
!! TAGTTTTGCGAGACAATATG+AGG + chr8_4:15337451-15337470 Msa1315780:three_prime_UTR 35.0%
GAATCACTCTCAACCAAAAA+CGG - chr8_4:15337488-15337507 None:intergenic 35.0%
TTTGAAGGTTCATTCATACG+CGG - chr8_4:15337669-15337688 None:intergenic 35.0%
! TGTTTTTCTCACACTCTGAC+TGG - chr8_4:15335106-15335125 None:intergenic 40.0%
ATACAGTGAGAGATAGTGAC+AGG + chr8_4:15335134-15335153 Msa1315780:five_prime_UTR 40.0%
! TTTTTCCACTGAGAATGAGG+AGG - chr8_4:15335211-15335230 None:intergenic 40.0%
TCTGTGTGTCATACTAAGGT+CGG + chr8_4:15335434-15335453 Msa1315780:intron 40.0%
!! GTTGAAGTGTTTGTTGTTGG+AGG + chr8_4:15335679-15335698 Msa1315780:five_prime_UTR 40.0%
! TGTTACCGAGTTGTGTGTTT+TGG + chr8_4:15336057-15336076 Msa1315780:CDS 40.0%
!! GATTCTGAAGGGGTTTTTCA+AGG + chr8_4:15336227-15336246 Msa1315780:three_prime_UTR 40.0%
TGAAGCCTAAGACTGTATCA+AGG + chr8_4:15336516-15336535 Msa1315780:three_prime_UTR 40.0%
GCAATGGAGGCAATCAAAAA+GGG + chr8_4:15336788-15336807 Msa1315780:three_prime_UTR 40.0%
! TAAGGTATCCAACTCAAGCA+CGG - chr8_4:15336957-15336976 None:intergenic 40.0%
CTCAATCGAATTGGATGCTA+AGG - chr8_4:15336975-15336994 None:intergenic 40.0%
TGTCAATGAAGCGAGTGAAA+TGG + chr8_4:15337030-15337049 Msa1315780:three_prime_UTR 40.0%
! TTCTCAGCAACAATTGACTC+AGG - chr8_4:15337109-15337128 None:intergenic 40.0%
! ACTGATCCCACTCTTGATTT+TGG + chr8_4:15337139-15337158 Msa1315780:three_prime_UTR 40.0%
TAACATTGACGATTTCGGAC+CGG + chr8_4:15337180-15337199 Msa1315780:three_prime_UTR 40.0%
!! GGACCGGATTTTGATGAATT+TGG + chr8_4:15337196-15337215 Msa1315780:three_prime_UTR 40.0%
TCAAAATCAGGAAGCTCAGT+AGG - chr8_4:15337274-15337293 None:intergenic 40.0%
! GGTGATGATGATGAATTTGC+TGG + chr8_4:15337307-15337326 Msa1315780:three_prime_UTR 40.0%
!! TGAATTTGCTGGTTGGATTG+AGG + chr8_4:15337318-15337337 Msa1315780:three_prime_UTR 40.0%
AGACACAAGGTATGTTGTGT+TGG - chr8_4:15337351-15337370 None:intergenic 40.0%
AGTAAAGTTCAGGAACCCTT+CGG - chr8_4:15337609-15337628 None:intergenic 40.0%
CAATGCGATTGAGGCCTAAA+AGG + chr8_4:15335879-15335898 Msa1315780:CDS 45.0%
!! TACCGAGTTGTGTGTTTTGG+AGG + chr8_4:15336060-15336079 Msa1315780:CDS 45.0%
AGCCTCCAAAACACACAACT+CGG - chr8_4:15336065-15336084 None:intergenic 45.0%
TCAGATACATCAGTGGCATC+AGG - chr8_4:15336386-15336405 None:intergenic 45.0%
CTCGTCTTCAGATACATCAG+TGG - chr8_4:15336393-15336412 None:intergenic 45.0%
GGCAATGGAGGCAATCAAAA+AGG + chr8_4:15336787-15336806 Msa1315780:three_prime_UTR 45.0%
TGTTCAATGCAGCAAGTCGA+GGG - chr8_4:15336832-15336851 None:intergenic 45.0%
TTGTTCAATGCAGCAAGTCG+AGG - chr8_4:15336833-15336852 None:intergenic 45.0%
TGATGTTCCATCAAACACCG+AGG + chr8_4:15337000-15337019 Msa1315780:three_prime_UTR 45.0%
! ACAAGGTATGTTGTGTTGGC+GGG - chr8_4:15337347-15337366 None:intergenic 45.0%
CACAAGGTATGTTGTGTTGG+CGG - chr8_4:15337348-15337367 None:intergenic 45.0%
AGTCTCTTAGGAGTGAGACT+AGG + chr8_4:15337380-15337399 Msa1315780:three_prime_UTR 45.0%
TTCCACTGAGAATGAGGAGG+AGG - chr8_4:15335208-15335227 None:intergenic 50.0%
GAAACTGCTGCAGGTACATG+AGG - chr8_4:15336470-15336489 None:intergenic 50.0%
GAAGTCAACGAAACTGCTGC+AGG - chr8_4:15336479-15336498 None:intergenic 50.0%
GTGGTTGGAGAAGTTGAGGT+TGG - chr8_4:15336590-15336609 None:intergenic 50.0%
ACCTCAACTTCTCCAACCAC+AGG + chr8_4:15336590-15336609 Msa1315780:three_prime_UTR 50.0%
CCTCAACTTCTCCAACCACA+GGG + chr8_4:15336591-15336610 Msa1315780:three_prime_UTR 50.0%
CGATCTTGTGCCAAATACCG+CGG + chr8_4:15336617-15336636 Msa1315780:three_prime_UTR 50.0%
CATACGAACACCGCGGTATT+TGG - chr8_4:15336630-15336649 None:intergenic 50.0%
AATACCGCGGTGTTCGTATG+AGG + chr8_4:15336630-15336649 Msa1315780:three_prime_UTR 50.0%
TATGAGGCCATGGGGCAAAT+GGG + chr8_4:15336646-15336665 Msa1315780:three_prime_UTR 50.0%
GTTCAATGCAGCAAGTCGAG+GGG - chr8_4:15336831-15336850 None:intergenic 50.0%
TCCAACTCAAGCACGGAAGA+AGG - chr8_4:15336950-15336969 None:intergenic 50.0%
TTCATTGACAGCAGCAGCCT+CGG - chr8_4:15337020-15337039 None:intergenic 50.0%
AGAGTGGGATCAGTCCCAAT+TGG - chr8_4:15337133-15337152 None:intergenic 50.0%
TGGAGACTTCGGTGACTTCA+AGG + chr8_4:15337216-15337235 Msa1315780:three_prime_UTR 50.0%
! CAAGGTATGTTGTGTTGGCG+GGG - chr8_4:15337346-15337365 None:intergenic 50.0%
TGCTGTTAGAGTCGTCCGAA+GGG + chr8_4:15337591-15337610 Msa1315780:three_prime_UTR 50.0%
CACTGAGAATGAGGAGGAGG+AGG - chr8_4:15335205-15335224 None:intergenic 55.0%
CTCCTCCTCCTCATTCTCAG+TGG + chr8_4:15335203-15335222 Msa1315780:five_prime_UTR 55.0%
GGGCTCTGTTGAAGAACCTC+TGG + chr8_4:15335771-15335790 Msa1315780:CDS 55.0%
CTGCTGCAGGTACATGAGGT+GGG - chr8_4:15336466-15336485 None:intergenic 55.0%
ACTGCTGCAGGTACATGAGG+TGG - chr8_4:15336467-15336486 None:intergenic 55.0%
CCCTGTGGTTGGAGAAGTTG+AGG - chr8_4:15336594-15336613 None:intergenic 55.0%
CAAGATCGTCTCCCTGTGGT+TGG - chr8_4:15336605-15336624 None:intergenic 55.0%
ATGGCCTCATACGAACACCG+CGG - chr8_4:15336637-15336656 None:intergenic 55.0%
CGGTGTTCGTATGAGGCCAT+GGG + chr8_4:15336637-15336656 Msa1315780:three_prime_UTR 55.0%
GGTGTTCGTATGAGGCCATG+GGG + chr8_4:15336638-15336657 Msa1315780:three_prime_UTR 55.0%
GTATGAGGCCATGGGGCAAA+TGG + chr8_4:15336645-15336664 Msa1315780:three_prime_UTR 55.0%
GCCTTCTTCCGTGCTTGAGT+TGG + chr8_4:15336946-15336965 Msa1315780:three_prime_UTR 55.0%
! GTGCTGTTAGAGTCGTCCGA+AGG + chr8_4:15337590-15337609 Msa1315780:three_prime_UTR 55.0%
GGCACAAGATCGTCTCCCTG+TGG - chr8_4:15336609-15336628 None:intergenic 60.0%
GCGGTGTTCGTATGAGGCCA+TGG + chr8_4:15336636-15336655 Msa1315780:three_prime_UTR 60.0%
TCAGCAGCCCATTTGCCCCA+TGG - chr8_4:15336656-15336675 None:intergenic 60.0%
!! GCAGCAGCCTCGGTGTTTGA+TGG - chr8_4:15337010-15337029 None:intergenic 60.0%
TCGAGGGGCTGCTGCAGAAG+CGG - chr8_4:15336816-15336835 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 15335099 15337694 15335099 ID=Msa1315780;Name=Msa1315780
chr8_4 mRNA 15335099 15337694 15335099 ID=Msa1315780-mRNA-1;Parent=Msa1315780;Name=Msa1315780-mRNA-1;_AED=0.27;_eAED=0.27;_QI=207|1|1|1|0|0|3|1554|83
chr8_4 exon 15335099 15335247 15335099 ID=Msa1315780-mRNA-1:exon:5886;Parent=Msa1315780-mRNA-1
chr8_4 exon 15335679 15335900 15335679 ID=Msa1315780-mRNA-1:exon:5887;Parent=Msa1315780-mRNA-1
chr8_4 exon 15336053 15337694 15336053 ID=Msa1315780-mRNA-1:exon:5888;Parent=Msa1315780-mRNA-1
chr8_4 five_prime_UTR 15335099 15335247 15335099 ID=Msa1315780-mRNA-1:five_prime_utr;Parent=Msa1315780-mRNA-1
chr8_4 five_prime_UTR 15335679 15335736 15335679 ID=Msa1315780-mRNA-1:five_prime_utr;Parent=Msa1315780-mRNA-1
chr8_4 CDS 15335737 15335900 15335737 ID=Msa1315780-mRNA-1:cds;Parent=Msa1315780-mRNA-1
chr8_4 CDS 15336053 15336140 15336053 ID=Msa1315780-mRNA-1:cds;Parent=Msa1315780-mRNA-1
chr8_4 three_prime_UTR 15336141 15337694 15336141 ID=Msa1315780-mRNA-1:three_prime_utr;Parent=Msa1315780-mRNA-1
Gene Sequence

>Msa1315780

ATGTCTTCTCAGTTTATTCTGGAATCAGTTGTTGGGGCTCTGTTGAAGAACCTCTGGATTTTCTCTGTTTCTTGTCTTCTGCTCACTCTTTCTGTGTTCTGTTCTGCTCTCTATATGCTCTTTGTCATCAAGAGCAATTTTTCAATGCGATTGAGGCCTAAAAGGACTTGTTACCGAGTTGTGTGTTTTGGAGGCTTTCATATACAGAAATTTTCTAATCTTTTTTTGTTCTTAAGAGTGGATCTCACTTGA
Protein sequence

>Msa1315780

MSSQFILESVVGALLKNLWIFSVSCLLLTLSVFCSALYMLFVIKSNFSMRLRPKRTCYRVVCFGGFHIQKFSNLFLFLRVDLT*