AlfalfaGEDB | Msa1235750

M. sativa cultivar zhongmu-4 / Msa1235750

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1235750	KEH18547.1	97.826	138	3	0	1	138	1	138	8.10e-90	271

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1235750	A0A072TMA7	97.826	138	3	0	1	138	1	138	3.87e-90	271

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0201070	Msa1235750	0.814258	1.688145e-51	-8.615850e-47
Msa0891040	Msa1235750	0.811906	5.537031e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1235750	MtrunA17_Chr8g0345761	97.826	138	3	0	1	138	57	194	7.66e-94	273
Msa1235750	MtrunA17_Chr8g0347771	95.035	141	4	1	1	138	1	141	4.66e-91	264

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1235750	AT4G05400.1	47.682	151	63	5	1	138	1	148	3.13e-33	117
Msa1235750	AT4G05400.2	47.682	151	63	5	1	138	1	148	3.13e-33	117
Msa1235750	AT4G05400.3	47.682	151	63	5	1	138	1	148	3.13e-33	117
Msa1235750	AT4G21140.1	48.592	142	67	4	1	138	1	140	8.39e-26	98.2

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Find 38 sgRNAs with CRISPR-Local

Find 342 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGTTATATAATCCAACCC+AGG	0.612178	8_2:+13934805	None:intergenic
ACCGGGTCCGAACCCACACC+TGG	0.460493	8_2:-13935051	Msa1235750:CDS
ACGTTATATAATCCAACCCA+GGG	0.597116	8_2:+13934806	None:intergenic
AGCCGCACCGCCAAAGGAAA+AGG	0.428181	8_2:-13935120	Msa1235750:CDS
AGCTTCCCGTGCGCGTTGTT+TGG	0.253091	8_2:+13934939	None:intergenic
AGTTGGTGCGGTGAAGATGA+CGG	0.654761	8_2:+13935162	None:intergenic
CAAACCCAAACAACGCGCAC+GGG	0.595471	8_2:-13934944	Msa1235750:CDS
CAACGATAATGGGAACCCCT+GGG	0.617131	8_2:-13934822	Msa1235750:CDS
CAGCGTTGGAGGAGTTGGTG+CGG	0.641635	8_2:+13935150	None:intergenic
CCAACGATAATGGGAACCCC+TGG	0.483698	8_2:-13934823	Msa1235750:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGCTGAACGAGAAAAATTA+GGG	-	chr8_2:13933117-13933136	Msa1235750:intron	30.0%
TCTGAAAAACCAACGATAAT+GGG	-	chr8_2:13933206-13933225	Msa1235750:intron	30.0%
GGTTGGATTATATAACGTTA+GGG	-	chr8_2:13933237-13933256	Msa1235750:intron	30.0%
ATGTATCAACCCTATTACAT+TGG	+	chr8_2:13933496-13933515	None:intergenic	30.0%
ACTCGTCTATGAAATCAAAT+AGG	-	chr8_2:13933738-13933757	Msa1235750:intron	30.0%
TCAAATAGGAACTGAACTTT+CGG	-	chr8_2:13933752-13933771	Msa1235750:intron	30.0%
GCAAAAACACCAAAACAAAA+TGG	-	chr8_2:13933852-13933871	Msa1235750:intron	30.0%
ATATCTAACATGCTTGAAGA+TGG	+	chr8_2:13933901-13933920	None:intergenic	30.0%
ATATTGAGATAACACTTTGG+TGG	+	chr8_2:13934034-13934053	None:intergenic	30.0%
ATATGATCACTTGAAAAGAG+TGG	-	chr8_2:13934050-13934069	Msa1235750:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	13932849	13935211	13932849	ID=Msa1235750;Name=Msa1235750
chr8_2	mRNA	13932849	13935211	13932849	ID=Msa1235750-mRNA-1;Parent=Msa1235750;Name=Msa1235750-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|141
chr8_2	exon	13934802	13935211	13934802	ID=Msa1235750-mRNA-1:exon:5360;Parent=Msa1235750-mRNA-1
chr8_2	exon	13932849	13932864	13932849	ID=Msa1235750-mRNA-1:exon:5359;Parent=Msa1235750-mRNA-1
chr8_2	CDS	13934802	13935211	13934802	ID=Msa1235750-mRNA-1:cds;Parent=Msa1235750-mRNA-1
chr8_2	CDS	13932849	13932864	13932849	ID=Msa1235750-mRNA-1:cds;Parent=Msa1235750-mRNA-1

Gene Sequence

>Msa1235750

ATGCTCCGTCTCACAAAGCTAAAACCACCGTCATCTTCACCGCACCAACTCCTCCAACGCTGCTCCGTCAGCCGCACCGCCAAAGGAAAAGGAAAAGCCGGTCAAATCCTAAAACGTTCCAAAATCACAGTAAAAAAAACCGGGTCCGAACCCACACCTGGAGCCCCAACCGGATCCCGCGAGAAACAAGAACGCGAACGTCTCTACGATCAATGCCTCAATGCACCAACACCGCTTCGTTTTCTCAAACCCAAACAACGCGCACGGGAAGCTGAACGAGAAAAATTAGGGTTAATTAGCAAAGATCGTCAAATTGAACTTGATATGATGAAGAAAAAAAACGCCAAATTTAGGGTTTCTGAAAAACCAACGATAATGGGAACCCCTGGGTTGGATTATATAACGTTAGGGCTTAGATTCCCATAG

Protein sequence

>Msa1235750

MLRLTKLKPPSSSPHQLLQRCSVSRTAKGKGKAGQILKRSKITVKKTGSEPTPGAPTGSREKQERERLYDQCLNAPTPLRFLKPKQRAREAEREKLGLISKDRQIELDMMKKKNAKFRVSEKPTIMGTPGLDYITLGLRFP*