AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0600680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 9 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGTTACTTCTTCTCTTTCA+TGG 0.301626 4_2:-42477572 None:intergenic
TGCTGCTGCAACTTCTGTTT+CGG 0.361471 4_2:+42474851 None:intergenic
ATTGCCTTTAGGGTCCATAT+TGG 0.409955 4_2:+42474774 None:intergenic
TGCAGCGCTGGTTGCAATGA+AGG 0.439173 4_2:+42477530 None:intergenic
AGAAGGAGTCTACTATTTCA+AGG 0.491554 4_2:-42477474 Msa0600680:intron
CAGTGGTCTGAAAATTGAAA+AGG 0.521123 4_2:-42477509 Msa0600680:CDS
CATTGCAACCAGCGCTGCAG+TGG 0.543575 4_2:-42477526 Msa0600680:CDS
AAAGGACAGCAAGAAGAAGA+AGG 0.546280 4_2:-42477491 Msa0600680:CDS
AACGCCAATATGGACCCTAA+AGG 0.590616 4_2:-42474778 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTGTTATTTATTTTTC+TGG - chr4_2:42476273-42476292 Msa0600680:intron 10.0%
!! ATAAATAAATTAAATTACTC+TGG - chr4_2:42477172-42477191 Msa0600680:intron 10.0%
!! TAGAGAAAAGAAAAAAAAAA+AGG + chr4_2:42475146-42475165 None:intergenic 15.0%
!!! TCTTATTGTTAATTTTCTTT+TGG - chr4_2:42475344-42475363 Msa0600680:intron 15.0%
!!! TAAGAATAATGGTTTTATAT+GGG + chr4_2:42475451-42475470 None:intergenic 15.0%
!!! ATAAGAATAATGGTTTTATA+TGG + chr4_2:42475452-42475471 None:intergenic 15.0%
!!! TTCTTTTTAATAGTATTTGA+TGG - chr4_2:42476679-42476698 Msa0600680:intron 15.0%
!!! TCTTTTTAATAGTATTTGAT+GGG - chr4_2:42476680-42476699 Msa0600680:intron 15.0%
!!! GTTAATTTAAACTTTAAAGT+TGG - chr4_2:42476790-42476809 Msa0600680:intron 15.0%
!! AAATTGATGAATGAATAATA+CGG + chr4_2:42477067-42477086 None:intergenic 15.0%
!! GTATCTGATAATAAGAATAA+TGG + chr4_2:42475462-42475481 None:intergenic 20.0%
!!! TATTTGTTCTTTTTTGTGTT+TGG - chr4_2:42476458-42476477 Msa0600680:intron 20.0%
!!! TGCATAAATATACTACATTT+TGG - chr4_2:42476507-42476526 Msa0600680:intron 20.0%
!! AATCAAATATGTTAGCTATT+TGG + chr4_2:42476535-42476554 None:intergenic 20.0%
!! AAATAGCTAACATATTTGAT+TGG - chr4_2:42476534-42476553 Msa0600680:intron 20.0%
!! AATAGCTAACATATTTGATT+GGG - chr4_2:42476535-42476554 Msa0600680:intron 20.0%
!! AATATATCTATCTGTTTGTT+GGG - chr4_2:42476731-42476750 Msa0600680:intron 20.0%
!!! TAATAACCAGATGATTTTAT+AGG - chr4_2:42476879-42476898 Msa0600680:intron 20.0%
!! TTATTCATTCATCAATTTCT+TGG - chr4_2:42477069-42477088 Msa0600680:intron 20.0%
!!! AGTAGTTTAACATTTTCTTA+AGG - chr4_2:42477263-42477282 Msa0600680:intron 20.0%
! CATTATCGTGAAAATTGAAA+AGG + chr4_2:42474924-42474943 None:intergenic 25.0%
! ATGATGAGATATTTGCAAAT+TGG - chr4_2:42475281-42475300 Msa0600680:intron 25.0%
! AGATACTCTTTCTTTATTGT+TGG - chr4_2:42475476-42475495 Msa0600680:intron 25.0%
!!! AATGAGATGATTTTCAAGAA+TGG - chr4_2:42475583-42475602 Msa0600680:intron 25.0%
! CACTCATAAAATAGAAATGA+CGG + chr4_2:42475685-42475704 None:intergenic 25.0%
! GTTGGGCTTATAATATATTT+CGG - chr4_2:42476005-42476024 Msa0600680:intron 25.0%
! AAAATTGAAGTCTATGTTTG+AGG - chr4_2:42476180-42476199 Msa0600680:intron 25.0%
! AAATTGAAGTCTATGTTTGA+GGG - chr4_2:42476181-42476200 Msa0600680:intron 25.0%
!!! TGTTAGACAAACTTTTTCTA+AGG - chr4_2:42476326-42476345 Msa0600680:intron 25.0%
!!! AATATGTTTTAGACGTTCAT+AGG + chr4_2:42476442-42476461 None:intergenic 25.0%
! TAAAAAGAAAAACAGCCATA+TGG + chr4_2:42476668-42476687 None:intergenic 25.0%
! GAATATATCTATCTGTTTGT+TGG - chr4_2:42476730-42476749 Msa0600680:intron 25.0%
! AGTTATGAAGAATGTGTATA+CGG + chr4_2:42477014-42477033 None:intergenic 25.0%
! TACACATTCTTCATAACTTA+AGG - chr4_2:42477016-42477035 Msa0600680:intron 25.0%
AGTCTACTATTTCAAGGTAT+GGG - chr4_2:42474879-42474898 Msa0600680:CDS 30.0%
AAATTGGTTGTAATCAGTGA+AGG - chr4_2:42475297-42475316 Msa0600680:intron 30.0%
AATTGGTTGTAATCAGTGAA+GGG - chr4_2:42475298-42475317 Msa0600680:intron 30.0%
!! TTGGATGTTCTGTTTCTATT+TGG - chr4_2:42475363-42475382 Msa0600680:intron 30.0%
! TCTTTATTGTTGGTCTAATG+AGG - chr4_2:42475486-42475505 Msa0600680:intron 30.0%
! AAACCTTGTCTAAGCTTTTT+CGG + chr4_2:42475517-42475536 None:intergenic 30.0%
! AAAAAGCTTAGACAAGGTTT+TGG - chr4_2:42475517-42475536 Msa0600680:intron 30.0%
!!! GACAAGGTTTTGGTTTAATT+TGG - chr4_2:42475527-42475546 Msa0600680:intron 30.0%
TAATTTGGCATGCTGTAATT+TGG - chr4_2:42475542-42475561 Msa0600680:intron 30.0%
AATTTGGCATGCTGTAATTT+GGG - chr4_2:42475543-42475562 Msa0600680:intron 30.0%
AGAAAATTAAGGTTCAGTCT+TGG - chr4_2:42475632-42475651 Msa0600680:intron 30.0%
GAAAATTAAGGTTCAGTCTT+GGG - chr4_2:42475633-42475652 Msa0600680:intron 30.0%
! AAATGACGGAATTTACAACT+GGG + chr4_2:42475671-42475690 None:intergenic 30.0%
! CGTCATTTCTATTTTATGAG+TGG - chr4_2:42475683-42475702 Msa0600680:intron 30.0%
! TTCTATTTTATGAGTGGTGT+TGG - chr4_2:42475689-42475708 Msa0600680:intron 30.0%
!! TCTAAGTTCTGATGTTGTTT+TGG + chr4_2:42476138-42476157 None:intergenic 30.0%
! TTTAGAATGTGCTAGCATTT+AGG - chr4_2:42476247-42476266 Msa0600680:intron 30.0%
AGAGGTTGTGAAAAACAAAT+TGG - chr4_2:42476368-42476387 Msa0600680:intron 30.0%
CTAACATATTTGATTGGGAT+TGG - chr4_2:42476540-42476559 Msa0600680:intron 30.0%
AGCATGTGTATCTTTACTTA+GGG + chr4_2:42476604-42476623 None:intergenic 30.0%
AAGCATGTGTATCTTTACTT+AGG + chr4_2:42476605-42476624 None:intergenic 30.0%
! ACACATGCTTTTACATTTGT+AGG - chr4_2:42476615-42476634 Msa0600680:intron 30.0%
TCTCTTCATTATGTATCTCT+TGG - chr4_2:42476768-42476787 Msa0600680:intron 30.0%
TTGGAACCTATAAAATCATC+TGG + chr4_2:42476888-42476907 None:intergenic 30.0%
! TAAAGGATGAGTCAAGATTT+TGG - chr4_2:42476938-42476957 Msa0600680:intron 30.0%
!!! GATTTTGGTTAAGAATTCCA+AGG - chr4_2:42476953-42476972 Msa0600680:intron 30.0%
!! TTAACAACCTTAAAAGCACT+TGG + chr4_2:42477304-42477323 None:intergenic 30.0%
!!! TTTTTTTGGTGGTTTAGGTA+TGG + chr4_2:42477551-42477570 None:intergenic 30.0%
CAGTGGTCTGAAAATTGAAA+AGG - chr4_2:42474838-42474857 Msa0600680:CDS 35.0%
AGAAGGAGTCTACTATTTCA+AGG - chr4_2:42474873-42474892 Msa0600680:CDS 35.0%
GAGTCTACTATTTCAAGGTA+TGG - chr4_2:42474878-42474897 Msa0600680:CDS 35.0%
ATTGGTTGTAATCAGTGAAG+GGG - chr4_2:42475299-42475318 Msa0600680:intron 35.0%
TGGGATGAACGAAATTCTAA+CGG + chr4_2:42475432-42475451 None:intergenic 35.0%
ATGCTGTAATTTGGGTGATT+TGG - chr4_2:42475551-42475570 Msa0600680:intron 35.0%
! TGTAATTTGGGTGATTTGGA+AGG - chr4_2:42475555-42475574 Msa0600680:intron 35.0%
TGTAACTGATGTAGAGGATA+TGG - chr4_2:42475606-42475625 Msa0600680:intron 35.0%
! GGATATGGTGGAGAAAATTA+AGG - chr4_2:42475621-42475640 Msa0600680:intron 35.0%
! GAAATGACGGAATTTACAAC+TGG + chr4_2:42475672-42475691 None:intergenic 35.0%
TCGTCTTAGTTGTTATGTGT+TGG - chr4_2:42475746-42475765 Msa0600680:intron 35.0%
CGTCTTAGTTGTTATGTGTT+GGG - chr4_2:42475747-42475766 Msa0600680:intron 35.0%
!! CTGTTAGTTTGTTTCTGAGA+CGG - chr4_2:42475797-42475816 Msa0600680:intron 35.0%
!! TGTTAGTTTGTTTCTGAGAC+GGG - chr4_2:42475798-42475817 Msa0600680:intron 35.0%
!!! TTATAGGCAGCTGTTTTTAG+TGG - chr4_2:42475859-42475878 Msa0600680:intron 35.0%
! CTAATTGCTTTCTGGTGTAT+TGG - chr4_2:42475935-42475954 Msa0600680:intron 35.0%
!! GCTTTGTTTGGTACTTGAAT+TGG + chr4_2:42476071-42476090 None:intergenic 35.0%
TTGTGCAAAGAATGTGGTAA+GGG + chr4_2:42476110-42476129 None:intergenic 35.0%
ATTGTGCAAAGAATGTGGTA+AGG + chr4_2:42476111-42476130 None:intergenic 35.0%
GACAAATTGTGCAAAGAATG+TGG + chr4_2:42476116-42476135 None:intergenic 35.0%
ATTGAGGACCAAATTTGAGA+AGG - chr4_2:42476204-42476223 Msa0600680:intron 35.0%
CTACGTTTCCTTCTCAAATT+TGG + chr4_2:42476215-42476234 None:intergenic 35.0%
!! TTTGTTTTTCACAACCTCTC+TGG + chr4_2:42476367-42476386 None:intergenic 35.0%
GCATGTGTATCTTTACTTAG+GGG + chr4_2:42476603-42476622 None:intergenic 35.0%
GCTTACTTATTCTCTCCATA+TGG - chr4_2:42476650-42476669 Msa0600680:intron 35.0%
TAGTATTTGATGGGACTGTT+TGG - chr4_2:42476689-42476708 Msa0600680:intron 35.0%
! ATCTGTTTGTTGGGTATGTT+TGG - chr4_2:42476740-42476759 Msa0600680:intron 35.0%
TTTGTTGGGTATGTTTGGTT+TGG - chr4_2:42476745-42476764 Msa0600680:intron 35.0%
!! CTTTCAAGTTCTCTGAGTTA+AGG + chr4_2:42477044-42477063 None:intergenic 35.0%
! TGAAATGCCAAGTGCTTTTA+AGG - chr4_2:42477294-42477313 Msa0600680:intron 35.0%
!! TTGAAGTGTGTACTGATTCT+AGG - chr4_2:42477358-42477377 Msa0600680:intron 35.0%
AAAGGACAGCAAGAAGAAGA+AGG - chr4_2:42474856-42474875 Msa0600680:CDS 40.0%
GAAACACATAGATCAAGACG+AGG + chr4_2:42475043-42475062 None:intergenic 40.0%
!! GTGTTCTTGTGTTGCTTTCT+TGG - chr4_2:42475085-42475104 Msa0600680:intron 40.0%
!! TTCTTGTGTTGCTTTCTTGG+AGG - chr4_2:42475088-42475107 Msa0600680:intron 40.0%
GATGAACGAAATTCTAACGG+TGG + chr4_2:42475429-42475448 None:intergenic 40.0%
AGACCGAAAAAGCTTAGACA+AGG - chr4_2:42475511-42475530 Msa0600680:intron 40.0%
GAATGGTGTAACTGATGTAG+AGG - chr4_2:42475600-42475619 Msa0600680:intron 40.0%
AACTGATGTAGAGGATATGG+TGG - chr4_2:42475609-42475628 Msa0600680:intron 40.0%
TTAAGGTTCAGTCTTGGGTT+TGG - chr4_2:42475638-42475657 Msa0600680:intron 40.0%
!! GTTAGTTTGTTTCTGAGACG+GGG - chr4_2:42475799-42475818 Msa0600680:intron 40.0%
!! TTAGTTTGTTTCTGAGACGG+GGG - chr4_2:42475800-42475819 Msa0600680:intron 40.0%
GCCTATAAACACAACCAACA+GGG + chr4_2:42475847-42475866 None:intergenic 40.0%
TGCCTATAAACACAACCAAC+AGG + chr4_2:42475848-42475867 None:intergenic 40.0%
CAAATCCACAACACCACAAA+CGG + chr4_2:42475914-42475933 None:intergenic 40.0%
GGATTTGGCTAATTGCTTTC+TGG - chr4_2:42475927-42475946 Msa0600680:intron 40.0%
TGCCTATCTTAAGTGGTGTT+GGG - chr4_2:42475988-42476007 Msa0600680:intron 40.0%
!! CAATCTCGGAAAGCTTTGTT+TGG + chr4_2:42476083-42476102 None:intergenic 40.0%
! GTGGTAAGGGTTTTCAATCT+CGG + chr4_2:42476097-42476116 None:intergenic 40.0%
AGTCTATGTTTGAGGGATTG+AGG - chr4_2:42476188-42476207 Msa0600680:intron 40.0%
!!! TTTTCTGGTTCGCTTTACTG+AGG - chr4_2:42476288-42476307 Msa0600680:intron 40.0%
!! TTTCTGGTTCGCTTTACTGA+GGG - chr4_2:42476289-42476308 Msa0600680:intron 40.0%
! ACTTTCAAAGTGTTCCAGAG+AGG - chr4_2:42476350-42476369 Msa0600680:intron 40.0%
TGAGCATGAGATAGAAGACT+TGG + chr4_2:42476418-42476437 None:intergenic 40.0%
TAGTTGTCATGTTTGCTCCT+TGG + chr4_2:42476973-42476992 None:intergenic 40.0%
AATGTGTATACGGTTCACTG+AGG + chr4_2:42477004-42477023 None:intergenic 40.0%
!!! TGGCGTTTTTTTGGTGGTTT+AGG + chr4_2:42477556-42477575 None:intergenic 40.0%
!!! CCATATTGGCGTTTTTTTGG+TGG + chr4_2:42477562-42477581 None:intergenic 40.0%
CCACCAAAAAAACGCCAATA+TGG - chr4_2:42477559-42477578 Msa0600680:exon 40.0%
!!! GGTCCATATTGGCGTTTTTT+TGG + chr4_2:42477565-42477584 None:intergenic 40.0%
GAACGAAATTCTAACGGTGG+CGG + chr4_2:42475426-42475445 None:intergenic 45.0%
ACCGAATTAGACAATCCCCA+CGG + chr4_2:42475718-42475737 None:intergenic 45.0%
AAACACAACCAACAGGGCAA+GGG + chr4_2:42475841-42475860 None:intergenic 45.0%
TAAACACAACCAACAGGGCA+AGG + chr4_2:42475842-42475861 None:intergenic 45.0%
! GCCCTGTTGGTTGTGTTTAT+AGG - chr4_2:42475843-42475862 Msa0600680:intron 45.0%
!!! TAGGCAGCTGTTTTTAGTGG+TGG - chr4_2:42475862-42475881 Msa0600680:intron 45.0%
ACCACAAACGGTATGGAACA+GGG + chr4_2:42475902-42475921 None:intergenic 45.0%
!! CGTTTGTGGTGTTGTGGATT+TGG - chr4_2:42475912-42475931 Msa0600680:intron 45.0%
CCACTTAAGATAGGCAGCTA+GGG + chr4_2:42475984-42476003 None:intergenic 45.0%
ACCACTTAAGATAGGCAGCT+AGG + chr4_2:42475985-42476004 None:intergenic 45.0%
CTGCCTATCTTAAGTGGTGT+TGG - chr4_2:42475987-42476006 Msa0600680:intron 45.0%
AGCCCAACACCACTTAAGAT+AGG + chr4_2:42475993-42476012 None:intergenic 45.0%
!! TTCTGGTTCGCTTTACTGAG+GGG - chr4_2:42476290-42476309 Msa0600680:intron 45.0%
! AGGCGCTACATGAAAGGTTT+TGG + chr4_2:42476907-42476926 None:intergenic 45.0%
CTTTCATGTAGCGCCTTCAA+GGG - chr4_2:42476911-42476930 Msa0600680:intron 45.0%
TTTCATGTAGCGCCTTCAAG+GGG - chr4_2:42476912-42476931 Msa0600680:intron 45.0%
CATCCTTTAACACCCCTTGA+AGG + chr4_2:42476927-42476946 None:intergenic 45.0%
TGCTGCTGCAACTTCTGTTT+CGG + chr4_2:42477499-42477518 None:intergenic 45.0%
!!! AAATTTTTTTTATTTCAATA+TGG + chr4_2:42475256-42475275 None:intergenic 5.0%
CCGAATTAGACAATCCCCAC+GGG + chr4_2:42475717-42475736 None:intergenic 50.0%
CCCGTGGGGATTGTCTAATT+CGG - chr4_2:42475714-42475733 Msa0600680:intron 50.0%
GCGTGCAAGTAGTCTGATGT+GGG - chr4_2:42475769-42475788 Msa0600680:intron 50.0%
CGTGCAAGTAGTCTGATGTG+GGG - chr4_2:42475770-42475789 Msa0600680:intron 50.0%
! TTCTTTTCCCCTTGCCCTGT+TGG - chr4_2:42475830-42475849 Msa0600680:intron 50.0%
AACACAACCAACAGGGCAAG+GGG + chr4_2:42475840-42475859 None:intergenic 50.0%
TCCCTGTTCCATACCGTTTG+TGG - chr4_2:42475898-42475917 Msa0600680:intron 50.0%
CACCACAAACGGTATGGAAC+AGG + chr4_2:42475903-42475922 None:intergenic 50.0%
CCACAACACCACAAACGGTA+TGG + chr4_2:42475909-42475928 None:intergenic 50.0%
! CCATACCGTTTGTGGTGTTG+TGG - chr4_2:42475906-42475925 Msa0600680:intron 50.0%
CCCTAGCTGCCTATCTTAAG+TGG - chr4_2:42475981-42476000 Msa0600680:intron 50.0%
!! CCTTGAAGGCGCTACATGAA+AGG + chr4_2:42476913-42476932 None:intergenic 50.0%
CCTTTCATGTAGCGCCTTCA+AGG - chr4_2:42476910-42476929 Msa0600680:intron 50.0%
! ATACGGTTCACTGAGGCATC+TGG + chr4_2:42476997-42477016 None:intergenic 50.0%
!! TGCAGCGCTGGTTGCAATGA+AGG + chr4_2:42474820-42474839 None:intergenic 55.0%
! TTTTCAGACCACTGCAGCGC+TGG + chr4_2:42474832-42474851 None:intergenic 55.0%
! ATGAGTGGTGTTGGAACCCG+TGG - chr4_2:42475698-42475717 Msa0600680:intron 55.0%
! TGAGTGGTGTTGGAACCCGT+GGG - chr4_2:42475699-42475718 Msa0600680:intron 55.0%
GGCGTGCAAGTAGTCTGATG+TGG - chr4_2:42475768-42475787 Msa0600680:intron 55.0%
!! GCGCCTTCAAGGGGTGTTAA+AGG - chr4_2:42476921-42476940 Msa0600680:intron 55.0%
CATTGCAACCAGCGCTGCAG+TGG - chr4_2:42474821-42474840 Msa0600680:CDS 60.0%
! GAGTGGTGTTGGAACCCGTG+GGG - chr4_2:42475700-42475719 Msa0600680:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 42474781 42477588 42474781 ID=Msa0600680;Name=Msa0600680
chr4_2 mRNA 42474781 42477588 42474781 ID=Msa0600680-mRNA-1;Parent=Msa0600680;Name=Msa0600680-mRNA-1;_AED=0.65;_eAED=0.65;_QI=13|0|0.5|0.5|1|1|2|0|72
chr4_2 exon 42474781 42474898 42474781 ID=Msa0600680-mRNA-1:exon:9227;Parent=Msa0600680-mRNA-1
chr4_2 exon 42477475 42477588 42477475 ID=Msa0600680-mRNA-1:exon:9226;Parent=Msa0600680-mRNA-1
chr4_2 five_prime_UTR 42477576 42477588 42477576 ID=Msa0600680-mRNA-1:five_prime_utr;Parent=Msa0600680-mRNA-1
chr4_2 CDS 42477475 42477575 42477475 ID=Msa0600680-mRNA-1:cds;Parent=Msa0600680-mRNA-1
chr4_2 CDS 42474781 42474898 42474781 ID=Msa0600680-mRNA-1:cds;Parent=Msa0600680-mRNA-1
Gene Sequence

>Msa0600680

ATGGCAGTTCTTCTTCTTCATCTCCTTCATTGCAACCAGCGCTGCAGTGGTCTGAAAATTGAAAAGGACAGCAAGAAGAAGAAGGAGTCTACTATTTCAAGTGTAGACAGCAAACAACGAAAGATGCCGAAACAGAAGTTGCAGCAGCATCATACAGCTGCTGTCATACAGCTTACTGCCATACCTAAACCACCAAAAAAACGCCAATATGGACCCTAA

Protein sequence

>Msa0600680

MAVLLLHLLHCNQRCSGLKIEKDSKKKKESTISSVDSKQRKMPKQKLQQHHTAAVIQLTAIPKPPKKRQYGP*