AlfalfaGEDB | Msa1245480

M. sativa cultivar zhongmu-4 / Msa1245480

KEGG: K09285
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006109 GO:0006110 GO:0006140 GO:0006355 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009743 GO:0009744 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0019219 GO:0019220 GO:0019222 GO:0019432 GO:0030808 GO:0030811 GO:0031323 GO:0031326 GO:0031329 GO:0034285 GO:0042221 GO:0042325 GO:0043467 GO:0043470 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0048518 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051174 GO:0051193 GO:0051196 GO:0051252 GO:0060255 GO:0062012 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1900371 GO:1900542 GO:1901363 GO:1901576 GO:1901700 GO:1901957 GO:1901959 GO:1903506 GO:1903578 GO:2000112 GO:2001141 GO:2001169
PFAM: PF00847.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1245480	AET02608.2	95.943	419	14	2	1	418	1	417	0.0	835

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1245480	sp\|Q6X5Y6\|WRI1_ARATH	53.646	384	132	15	21	376	27	392	4.14e-98	302

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1245480	G7L700	95.943	419	14	2	1	418	1	417	0.0	835

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TFs/TRs:

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Gene ID	Type	Classification
Msa1245480	TF	AP2/ERF-AP2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0067590	Msa1245480	-0.805700	1.176300e-49	-8.615850e-47
Msa0178000	Msa1245480	0.803308	3.709137e-49	-8.615850e-47
Msa0334450	Msa1245480	-0.800721	1.261956e-48	-8.615850e-47
Msa1245480	Msa1322560	0.850504	1.627520e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1245480	MtrunA17_Chr8g0355191	95.943	419	14	2	1	418	1	417	0.0	835
Msa1245480	MtrunA17_Chr8g0355181	72.236	407	90	10	1	404	1	387	0.0	528
Msa1245480	MtrunA17_Chr4g0073521	76.023	171	41	0	56	226	46	216	2.00e-95	290
Msa1245480	MtrunA17_Chr7g0216601	62.555	227	68	3	48	271	56	268	7.20e-92	282
Msa1245480	MtrunA17_Chr4g0002011	59.592	245	68	5	52	265	31	275	1.24e-86	266
Msa1245480	MtrunA17_Chr6g0452911	69.318	176	44	1	61	226	65	240	1.44e-83	260
Msa1245480	MtrunA17_Chr8g0362541	66.832	202	52	2	56	243	68	268	2.41e-80	253
Msa1245480	MtrunA17_Chr5g0400871	65.934	182	57	2	43	221	237	416	1.47e-77	250
Msa1245480	MtrunA17_Chr2g0328971	54.661	236	93	4	5	228	105	338	1.32e-76	247
Msa1245480	MtrunA17_Chr4g0072291	66.864	169	53	1	56	221	158	326	2.56e-76	246

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1245480	AT3G54320.1	53.646	384	132	15	21	376	27	392	4.14e-99	303
Msa1245480	AT3G54320.3	53.646	384	132	15	21	376	27	392	4.21e-99	302
Msa1245480	AT1G79700.2	63.274	226	71	4	45	260	37	260	3.24e-78	245
Msa1245480	AT5G57390.2	60.577	208	74	4	17	221	47	249	5.63e-78	248
Msa1245480	AT5G57390.2	38.835	103	47	2	153	247	79	173	1.03e-11	67.0
Msa1245480	AT5G57390.1	60.476	210	75	4	15	221	163	367	4.76e-77	249
Msa1245480	AT5G57390.1	38.835	103	47	2	153	247	197	291	8.93e-12	67.4
Msa1245480	AT1G16060.1	69.841	189	53	2	45	230	43	230	1.01e-75	239
Msa1245480	AT1G51190.1	64.607	178	60	1	56	230	186	363	4.53e-75	244
Msa1245480	AT1G51190.1	40.741	108	48	3	147	246	178	277	2.39e-12	68.9

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Find 84 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCAAGATGCCGGAGATA+TGG	0.435000	8_2:+35591378	Msa1245480:CDS
AACAAGATTCAGAACAAGAA+GGG	0.581787	8_2:+35586510	Msa1245480:CDS
AACACGGAAGAACCTTGTGG+TGG	0.703150	8_2:+35591289	Msa1245480:CDS
AACTCAAGAAGAGGCAGCAG+TGG	0.504028	8_2:+35590811	Msa1245480:CDS
AACTTATGCAAAAGAGTTTG+AGG	0.450069	8_2:+35587945	Msa1245480:CDS
AAGAGTATTTGAACTCTTTG+CGG	0.450951	8_2:+35587989	Msa1245480:CDS
AAGATTTGAAGCTCATCTAT+GGG	0.572069	8_2:+35586473	Msa1245480:CDS
ACAATTTCTGTGTTTGATTG+TGG	0.530549	8_2:-35590950	None:intergenic
ACTGTAGTGGTTGAAGATGA+TGG	0.521228	8_2:-35591463	None:intergenic
AGAAGAAGCTCCATTTACAG+AGG	0.653025	8_2:+35586296	Msa1245480:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCATCATCTTCATCTTCTAT+TGG	+	chr8_2:35586158-35586177	Msa1245480:CDS	30.0%
CAGAAGAAAAACCAAACAAA+TGG	+	chr8_2:35586266-35586285	Msa1245480:CDS	30.0%
AAGAAAAACCAAACAAATGG+TGG	+	chr8_2:35586269-35586288	Msa1245480:CDS	30.0%
CCCATCAACAAAATTCAAAA+GGG	-	chr8_2:35586391-35586410	None:intergenic	30.0%
ACCCATCAACAAAATTCAAA+AGG	-	chr8_2:35586392-35586411	None:intergenic	30.0%
AACAAGATTCAGAACAAGAA+GGG	+	chr8_2:35586510-35586529	Msa1245480:CDS	30.0%
GTTAAATTAGTCCTTGATCA+TGG	-	chr8_2:35587450-35587469	None:intergenic	30.0%
ATAACATTAATACATGGACC+TGG	+	chr8_2:35587491-35587510	Msa1245480:intron	30.0%
TGGAAAAAATTAGTTAGGAG+AGG	-	chr8_2:35587716-35587735	None:intergenic	30.0%
ATTCCTTAAATAGACGATCA+GGG	-	chr8_2:35587750-35587769	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	35586131	35591495	35586131	ID=Msa1245480;Name=Msa1245480
chr8_2	mRNA	35586131	35591495	35586131	ID=Msa1245480-mRNA-1;Parent=Msa1245480;Name=Msa1245480-mRNA-1;_AED=0.34;_eAED=0.35;_QI=0\|0.2\|0\|1\|1\|1\|6\|0\|418
chr8_2	exon	35586131	35586327	35586131	ID=Msa1245480-mRNA-1:exon:11283;Parent=Msa1245480-mRNA-1
chr8_2	exon	35586458	35586540	35586458	ID=Msa1245480-mRNA-1:exon:11284;Parent=Msa1245480-mRNA-1
chr8_2	exon	35587211	35587299	35587211	ID=Msa1245480-mRNA-1:exon:11285;Parent=Msa1245480-mRNA-1
chr8_2	exon	35587940	35588064	35587940	ID=Msa1245480-mRNA-1:exon:11286;Parent=Msa1245480-mRNA-1
chr8_2	exon	35588633	35588709	35588633	ID=Msa1245480-mRNA-1:exon:11287;Parent=Msa1245480-mRNA-1
chr8_2	exon	35590810	35591495	35590810	ID=Msa1245480-mRNA-1:exon:11288;Parent=Msa1245480-mRNA-1
chr8_2	CDS	35586131	35586327	35586131	ID=Msa1245480-mRNA-1:cds;Parent=Msa1245480-mRNA-1
chr8_2	CDS	35586458	35586540	35586458	ID=Msa1245480-mRNA-1:cds;Parent=Msa1245480-mRNA-1
chr8_2	CDS	35587211	35587299	35587211	ID=Msa1245480-mRNA-1:cds;Parent=Msa1245480-mRNA-1
chr8_2	CDS	35587940	35588064	35587940	ID=Msa1245480-mRNA-1:cds;Parent=Msa1245480-mRNA-1
chr8_2	CDS	35588633	35588709	35588633	ID=Msa1245480-mRNA-1:cds;Parent=Msa1245480-mRNA-1
chr8_2	CDS	35590810	35591495	35590810	ID=Msa1245480-mRNA-1:cds;Parent=Msa1245480-mRNA-1

Gene Sequence

>Msa1245480

ATGAAGAGGTCTCCACCATCTTCAAGTTCATCATCTTCATCTTCTATTGGATTCGAGAATCCAATTCAATCAGTGAAAAAAAAGGGTGCTAAGAATCCAAAAAAGAATCTCAATGTCAAATCACAGAATTTAAAGCAGAAGAAAAACCAAACAAATGGTGGCAGTAGAAGAAGCTCCATTTACAGAGGAGTTACAAGACATAGATGGACTGGAAGATTTGAAGCTCATCTATGGGATAAGACTTCTTGGAACAAGATTCAGAACAAGAAGGGAAAACAAGGTGCTTATGATACTGAAGAAGCAGCAGCTCGTACCTATGATCTTGCAGCACTCAAGTATTGGGGGAACGATGCAACCTTAAATTTTCCGATAGAAACTTATGCAAAAGAGTTTGAGGAAATGGACAAAGCCTCAAAAGAAGAGTATTTGAACTCTTTGCGGCGGCAAAGCAGTGGTTTTTCTAGAGGACTTTCTAAATACCGTGGGGTTGCCAGGCATCACCATAATGGTCGGTGGGAAGCTCGCATTGGAAGAGTTCGTGGTAACAAGTACCTCTATTTGGGCACATACAAAACTCAAGAAGAGGCAGCAGTGGCTTATGATATGGCAGCAATAAAGCACAGAGGAATCAATGCCGTGACCAATTTTGATATAAGCAACTACATGGACAAATTGAAGGTGGAGAAAAATGATGAAACAGAACAAACAAAACCACAATCAAACACAGAAATTGTTGCTAATTCCCCTGATTCCGAAGAAGCATTGGAAGAACAAACAACAACTACCCCGCCTCCACAAGAAAATCAACTACAAATAGAACCTTCGCAGCAACAATTTCAACAACATGTTCCTCCAACTAGTCATGAATGCGCAATAGTTAATATTATGGATCATTGTTTTGACCAAGATTATCCATGGAGCTTCATGTATAATGGATTGTCAGAGTTTCAAGACACAGGTTCGACTATGAGTAAGTGTGGTGATGATGATTTAATGAATATATTTGATGGCTCAAGCTTTGATGATAACATTGAATTTGAACTTATGTTCAACACGGAAGAACCTTGTGGTGGTCGTGGTGAAAGTGATATCAATATCGGTGAAGTTTTGGAGGGTATTGATCTTTGTGGAGGATTATTAAATCAAGATGCCGGAGATATGGTTGCGGATGAGAATAGTAACAAGGAGAAGGATTTATTATCCTTAGATTCTTCTTCTTCTTCCCCATCATCTTCAACCACTACAGTTTCTTTGTAA

Protein sequence

>Msa1245480

MKRSPPSSSSSSSSSIGFENPIQSVKKKGAKNPKKNLNVKSQNLKQKKNQTNGGSRRSSIYRGVTRHRWTGRFEAHLWDKTSWNKIQNKKGKQGAYDTEEAAARTYDLAALKYWGNDATLNFPIETYAKEFEEMDKASKEEYLNSLRRQSSGFSRGLSKYRGVARHHHNGRWEARIGRVRGNKYLYLGTYKTQEEAAVAYDMAAIKHRGINAVTNFDISNYMDKLKVEKNDETEQTKPQSNTEIVANSPDSEEALEEQTTTTPPPQENQLQIEPSQQQFQQHVPPTSHECAIVNIMDHCFDQDYPWSFMYNGLSEFQDTGSTMSKCGDDDLMNIFDGSSFDDNIEFELMFNTEEPCGGRGESDINIGEVLEGIDLCGGLLNQDAGDMVADENSNKEKDLLSLDSSSSSPSSSTTTVSL*