AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1250440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1250440 MtrunA17_Chr4g0054621 75.516 339 28 5 2 309 28 342 5.67e-174 485
Msa1250440 MtrunA17_Chr4g0054581 46.866 367 120 14 2 309 29 379 1.74e-78 243
Msa1250440 MtrunA17_Chr2g0307131 45.299 351 125 15 2 308 28 355 1.06e-69 220
Msa1250440 MtrunA17_Chr6g0468491 44.413 349 113 16 2 309 6 314 1.05e-68 216
Msa1250440 MtrunA17_Chr2g0307141 41.926 353 140 14 2 309 28 360 1.11e-64 207
Msa1250440 MtrunA17_Chr4g0033281 42.515 334 129 9 2 308 28 325 2.80e-64 206
Msa1250440 MtrunA17_Chr4g0033291 43.620 337 125 13 2 309 29 329 1.05e-63 204
Msa1250440 MtrunA17_Chr4g0033391 42.560 336 118 15 2 307 29 319 3.63e-61 197
Msa1250440 MtrunA17_Chr6g0468461 42.320 319 113 15 2 284 28 311 7.64e-50 169
Msa1250440 MtrunA17_Chr5g0400621 36.905 336 145 9 5 308 33 333 7.87e-42 147
Msa1250440 MtrunA17_Chr7g0225901 40.650 246 80 11 74 284 26 240 2.40e-36 130
Msa1250440 MtrunA17_Chr5g0400641 47.328 131 59 2 168 290 28 156 4.06e-31 114
Msa1250440 MtrunA17_Chr2g0280161 30.392 306 153 9 50 307 60 353 1.52e-26 107
Msa1250440 MtrunA17_Chr4g0002801 32.812 256 118 8 93 305 87 331 2.45e-26 106
Msa1250440 MtrunA17_Chr4g0002831 39.416 137 79 2 166 302 70 202 2.85e-26 103
Msa1250440 MtrunA17_Chr5g0400661 38.462 156 77 1 168 304 179 334 7.23e-26 105
Msa1250440 MtrunA17_Chr4g0002851 40.000 135 69 2 168 302 72 194 3.90e-25 99.8
Msa1250440 MtrunA17_Chr7g0233441 38.519 135 79 2 168 302 64 194 2.16e-24 97.8
Msa1250440 MtrunA17_Chr4g0049331 36.486 148 84 2 168 307 165 310 6.08e-21 90.9
Msa1250440 MtrunA17_Chr8g0336681 28.736 261 120 9 84 300 93 331 2.11e-20 90.1
Msa1250440 MtrunA17_Chr2g0333611 34.028 144 89 2 168 306 75 217 3.35e-20 87.0
Msa1250440 MtrunA17_Chr4g0002781 33.498 203 91 6 145 305 150 350 6.29e-20 89.0
Msa1250440 MtrunA17_Chr4g0054561 36.957 138 79 3 168 299 185 320 9.75e-20 87.8
Msa1250440 MtrunA17_Chr4g0054551 36.957 138 79 3 168 299 185 320 2.51e-19 86.7
Msa1250440 MtrunA17_Chr2g0333621 26.459 257 135 7 89 308 76 315 3.95e-18 83.2
Msa1250440 MtrunA17_Chr2g0333641 30.093 216 106 9 119 299 97 302 1.16e-17 82.0
Msa1250440 MtrunA17_Chr4g0054571 35.897 156 79 2 168 306 180 331 1.16e-17 82.0
Msa1250440 MtrunA17_Chr5g0400671 25.850 294 172 7 54 304 33 323 1.97e-17 81.3
Msa1250440 MtrunA17_Chr2g0333541 35.172 145 84 4 168 303 124 267 7.22e-16 76.3
Msa1250440 MtrunA17_Chr7g0249811 23.427 286 178 7 49 305 56 329 3.00e-14 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1250440 AT2G22770.1 33.333 156 83 1 168 302 162 317 1.23e-21 93.6
Msa1250440 AT2G22760.1 36.242 149 81 5 168 304 149 295 8.70e-21 90.9
Msa1250440 AT4G37850.2 28.514 249 133 7 96 307 88 328 7.26e-18 83.2
Msa1250440 AT4G37850.1 28.514 249 133 7 96 307 88 328 7.26e-18 83.2
Msa1250440 AT2G22760.2 35.938 128 69 4 168 284 149 274 6.19e-16 77.0
Msa1250440 AT2G22750.5 30.667 150 92 3 168 306 180 328 3.07e-11 63.9
Msa1250440 AT2G22750.3 30.667 150 92 3 168 306 184 332 3.08e-11 63.9
Msa1250440 AT2G22750.2 30.667 150 92 3 168 306 156 304 3.95e-11 63.2

Find 48 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGTGTCATTGCCCAATT+TGG 0.159846 8_2:-46102671 Msa1250440:CDS
AAAACGTGTGTTATCCAAAT+TGG 0.202416 8_2:+46102657 None:intergenic
CCAAACCCAACAGATTTATA+CGG 0.283498 8_2:-46102721 Msa1250440:CDS
CAAGAAGTCCATCATGCTTT+TGG 0.296888 8_2:+46101833 None:intergenic
CGTATAAATCTGTTGGGTTT+GGG 0.304768 8_2:+46102722 None:intergenic
AGGGTAGAAGCAAAAGTGTT+AGG 0.330735 8_2:-46101883 Msa1250440:CDS
ACAATAGTGGTCTGGTTTGT+TGG 0.341236 8_2:+46101939 None:intergenic
CCGTATAAATCTGTTGGGTT+TGG 0.355612 8_2:+46102721 None:intergenic
AATTTGTTGTGAAAAGCTTT+TGG 0.355869 8_2:+46102795 None:intergenic
GGCTAGTGCCATTGCCTTTG+AGG 0.361619 8_2:-46102959 Msa1250440:CDS
ATGCTGCCATGCGTAGATTC+TGG 0.364951 8_2:+46100073 None:intergenic
AACGCTCTTTAAGCTCTTTG+AGG 0.388426 8_2:+46102022 None:intergenic
TCATGTAAAAGATCACATTA+TGG 0.398549 8_2:-46102557 Msa1250440:intron
TTGGTGATGCTACTGCTATC+AGG 0.398588 8_2:-46101903 Msa1250440:CDS
CACTTTCAGTTTCACAATAG+TGG 0.411282 8_2:+46101926 None:intergenic
TCAGTTTCACAATAGTGGTC+TGG 0.454097 8_2:+46101931 None:intergenic
AAACGTGTGTTATCCAAATT+GGG 0.461557 8_2:+46102658 None:intergenic
GGGTAGAAGCAAAAGTGTTA+GGG 0.464493 8_2:-46101882 Msa1250440:CDS
TCAAACCCGTATAAATCTGT+TGG 0.488589 8_2:+46102715 None:intergenic
CAAACCCGTATAAATCTGTT+GGG 0.491045 8_2:+46102716 None:intergenic
ATCACAATCTAAGAGGTCTT+CGG 0.516297 8_2:-46102581 Msa1250440:CDS
CAAACCCAACAGATTTATAC+GGG 0.531352 8_2:-46102720 Msa1250440:CDS
CTAAAACATCACAATCTAAG+AGG 0.533656 8_2:-46102588 Msa1250440:CDS
GGGTTTGGGTTGTCAAAAGA+CGG 0.535552 8_2:+46102736 None:intergenic
AATCTCCAAAGGCTAAAATA+CGG 0.541965 8_2:+46101761 None:intergenic
GAATGTTTCGCTTAGAGTGG+TGG 0.560814 8_2:+46102906 None:intergenic
AGTCTCTCTTTGTTCCTCAA+AGG 0.562733 8_2:+46102945 None:intergenic
TGAGATGACTGAAAAGAGGA+TGG 0.564225 8_2:+46102763 None:intergenic
TCTTTGTTCCTCAAAGGCAA+TGG 0.566921 8_2:+46102951 None:intergenic
TGGTGATGCTACTGCTATCA+GGG 0.568737 8_2:-46101902 Msa1250440:CDS
ATCCACTGCCAAAAGCATGA+TGG 0.569741 8_2:-46101841 Msa1250440:CDS
ATTGAATGTTTCGCTTAGAG+TGG 0.570806 8_2:+46102903 None:intergenic
AATCTGAGATGACTGAAAAG+AGG 0.576642 8_2:+46102759 None:intergenic
ACTCTAAGCGAAACATTCAA+TGG 0.582474 8_2:-46102901 Msa1250440:CDS
CAGCAATGATTGTAATGTCG+TGG 0.596337 8_2:+46101734 None:intergenic
ATTGGGCAATGACACTGAAG+TGG 0.602756 8_2:+46102675 None:intergenic
AGAGTTTCTCTCACGTGACA+TGG 0.613188 8_2:-46102980 Msa1250440:CDS
TGAATACATGTTATTTGACA+AGG 0.622791 8_2:-46103040 None:intergenic
TTGGGCAATGACACTGAAGT+GGG 0.628306 8_2:+46102676 None:intergenic
ATATGTTAATTAGAAGGACA+AGG 0.637697 8_2:-46102073 Msa1250440:intron
CTGAAAAGAGGATGGAGATG+CGG 0.641434 8_2:+46102771 None:intergenic
CACAAAGGAAGATCAATCGA+TGG 0.644880 8_2:-46101968 Msa1250440:CDS
GAACTTGCTTGATGCTGACG+TGG 0.647274 8_2:-46103007 Msa1250440:CDS
CGACATTACAATCATTGCTG+AGG 0.658041 8_2:-46101731 Msa1250440:intron
TATTGTGAAACTGAAAGTGT+TGG 0.666038 8_2:-46101922 Msa1250440:CDS
ACTCGTCCAGAATCTACGCA+TGG 0.674291 8_2:-46100079 Msa1250440:CDS
AAAGGAAGATCAATCGATGG+TGG 0.686344 8_2:-46101965 Msa1250440:CDS
GTCGTGGACAGAATCTCCAA+AGG 0.686404 8_2:+46101750 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATTATATATAAAAACAAA+TGG + chr8_2:46100804-46100823 None:intergenic 10.0%
!! TATATAATAGTAAAAGAATA+TGG - chr8_2:46100814-46100833 Msa1250440:intron 10.0%
!! ATAATTTAAGAAAATTAAAG+TGG + chr8_2:46100957-46100976 None:intergenic 10.0%
!! TGTAAAATATGTTAATTAGA+AGG - chr8_2:46101007-46101026 Msa1250440:intron 15.0%
!!! CTTCTTTTTTATCTTTTTAT+TGG - chr8_2:46101632-46101651 Msa1250440:intron 15.0%
!! ACATCAAAATATTTAATGAT+TGG + chr8_2:46101747-46101766 None:intergenic 15.0%
!!! CACTCATTTTATAAAAAATT+AGG - chr8_2:46102536-46102555 Msa1250440:intron 15.0%
!! TCTTAATAAATAAATCACTA+TGG - chr8_2:46102673-46102692 Msa1250440:CDS 15.0%
!!! TCTAGAAATTTTTATATATG+GGG + chr8_2:46102699-46102718 None:intergenic 15.0%
!!! GTCTAGAAATTTTTATATAT+GGG + chr8_2:46102700-46102719 None:intergenic 15.0%
!! TAATACATGGTTAAAGATAT+AGG + chr8_2:46100718-46100737 None:intergenic 20.0%
!!! TTATAAGTTTTGCTAATACA+TGG + chr8_2:46100731-46100750 None:intergenic 20.0%
!!! GTTGTTATTATTCAACAAAT+GGG + chr8_2:46100865-46100884 None:intergenic 20.0%
!!! TCAATTTTAGAAGAAGAAAA+AGG - chr8_2:46101089-46101108 Msa1250440:intron 20.0%
!!! CAATTTTAGAAGAAGAAAAA+GGG - chr8_2:46101090-46101109 Msa1250440:intron 20.0%
!! ACAAAATAAACTCTATGATT+TGG + chr8_2:46101507-46101526 None:intergenic 20.0%
!! TAAAAAGTAATCAAGCTTAT+GGG + chr8_2:46101723-46101742 None:intergenic 20.0%
!! TTAAAAAGTAATCAAGCTTA+TGG + chr8_2:46101724-46101743 None:intergenic 20.0%
!! TTCTTATTGATCAAAAACAA+TGG + chr8_2:46101979-46101998 None:intergenic 20.0%
!! AATAAGAAGTGAGAAAAATA+AGG - chr8_2:46101991-46102010 Msa1250440:CDS 20.0%
!!! CTTAAATAATGAAAACGTTT+TGG + chr8_2:46102086-46102105 None:intergenic 20.0%
!!! TTTTTTGACAAAGAGAATAA+TGG + chr8_2:46102119-46102138 None:intergenic 20.0%
!! ATTATTCTCTTTGTCAAAAA+AGG - chr8_2:46102118-46102137 Msa1250440:intron 20.0%
!! TTATTCTCTTTGTCAAAAAA+GGG - chr8_2:46102119-46102138 Msa1250440:intron 20.0%
!! TATCATGAACTTAAATTGTA+GGG - chr8_2:46102224-46102243 Msa1250440:intron 20.0%
!!! TGAACTTTTCATAGTTTATA+TGG - chr8_2:46102295-46102314 Msa1250440:intron 20.0%
!!! CATTCTCTTTAAAAAAACAA+TGG + chr8_2:46102423-46102442 None:intergenic 20.0%
!!! AAAAATTAGGTCCATATTTT+AGG - chr8_2:46102549-46102568 Msa1250440:intron 20.0%
!! TAGAATCTATTCCTAAAATA+TGG + chr8_2:46102563-46102582 None:intergenic 20.0%
!! TTATTAGATGGAATTCATAT+AGG + chr8_2:46102601-46102620 None:intergenic 20.0%
!!! ATATTCACTCTTTTATTAGA+TGG + chr8_2:46102613-46102632 None:intergenic 20.0%
!!! GGTCTAGAAATTTTTATATA+TGG + chr8_2:46102701-46102720 None:intergenic 20.0%
!! TAACAATCAATACAAATAAG+TGG + chr8_2:46102935-46102954 None:intergenic 20.0%
!!! TCTATTATTGTTTTACAGAT+TGG - chr8_2:46102957-46102976 Msa1250440:CDS 20.0%
! ATCAAAAAATTTCAGCTTCT+TGG + chr8_2:46100255-46100274 None:intergenic 25.0%
!!! AATTTGTTGTGAAAAGCTTT+TGG + chr8_2:46100294-46100313 None:intergenic 25.0%
!!! TTTTTTGAAGACTCAATTGA+GGG + chr8_2:46100464-46100483 None:intergenic 25.0%
!!! ATTTTTTGAAGACTCAATTG+AGG + chr8_2:46100465-46100484 None:intergenic 25.0%
! TCATGTAAAAGATCACATTA+TGG - chr8_2:46100529-46100548 Msa1250440:intron 25.0%
! TATAGGTTAAAGATGTGTAT+TGG + chr8_2:46100701-46100720 None:intergenic 25.0%
!! TAATGTTAGATTTGTTTCGA+CGG + chr8_2:46100776-46100795 None:intergenic 25.0%
!! CGTTGTTATTATTCAACAAA+TGG + chr8_2:46100866-46100885 None:intergenic 25.0%
! ATATGTTAATTAGAAGGACA+AGG - chr8_2:46101013-46101032 Msa1250440:intron 25.0%
! AAGATGATGTAGAGAATAAA+TGG + chr8_2:46101430-46101449 None:intergenic 25.0%
!!! TTTTCATAAGTTGAGATTGT+GGG + chr8_2:46101840-46101859 None:intergenic 25.0%
!!! AACCACTTTTTGATAACTTT+TGG - chr8_2:46101894-46101913 Msa1250440:CDS 25.0%
! ATCCAAAAGTTATCAAAAAG+TGG + chr8_2:46101899-46101918 None:intergenic 25.0%
! GTATCATGAACTTAAATTGT+AGG - chr8_2:46102223-46102242 Msa1250440:intron 25.0%
! ATGTTTAAGCGATAAAATGA+TGG + chr8_2:46102449-46102468 None:intergenic 25.0%
!! ATAACTTTTCTCTTTTTGTG+TGG - chr8_2:46102647-46102666 Msa1250440:CDS 25.0%
! TGAATGAGAAAGTTGTTTAA+TGG - chr8_2:46102885-46102904 Msa1250440:CDS 25.0%
AAAAATTTCAGCTTCTTGGT+TGG + chr8_2:46100251-46100270 None:intergenic 30.0%
AAACGTGTGTTATCCAAATT+GGG + chr8_2:46100431-46100450 None:intergenic 30.0%
AAAACGTGTGTTATCCAAAT+TGG + chr8_2:46100432-46100451 None:intergenic 30.0%
GTCTTCAAAAAATCAGAACT+TGG - chr8_2:46100472-46100491 Msa1250440:intron 30.0%
CTAAAACATCACAATCTAAG+AGG - chr8_2:46100498-46100517 Msa1250440:intron 30.0%
AGTTACTTCTTACCTTCTTT+AGG + chr8_2:46100619-46100638 None:intergenic 30.0%
! TATTGTGAAACTGAAAGTGT+TGG - chr8_2:46101164-46101183 Msa1250440:intron 30.0%
! TAGGGAAAAAGATGTTGATT+CGG - chr8_2:46101222-46101241 Msa1250440:intron 30.0%
!!! AATAACCGTATTTTAGCCTT+TGG - chr8_2:46101320-46101339 Msa1250440:intron 30.0%
AATCTCCAAAGGCTAAAATA+CGG + chr8_2:46101328-46101347 None:intergenic 30.0%
!! ATTTTGATGTTTTGCTCTAG+AGG - chr8_2:46101757-46101776 Msa1250440:CDS 30.0%
! TTTCATAAGTTGAGATTGTG+GGG + chr8_2:46101839-46101858 None:intergenic 30.0%
!! GTTTTCATAAGTTGAGATTG+TGG + chr8_2:46101841-46101860 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG + chr8_2:46101966-46101985 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+TGG - chr8_2:46102017-46102036 Msa1250440:CDS 30.0%
! AAGCTTGCAGTTTGTATTTT+GGG + chr8_2:46102187-46102206 None:intergenic 30.0%
! AAAGCTTGCAGTTTGTATTT+TGG + chr8_2:46102188-46102207 None:intergenic 30.0%
AGGGCTTAATACAAGTAAAA+AGG - chr8_2:46102243-46102262 Msa1250440:intron 30.0%
GGGCTTAATACAAGTAAAAA+GGG - chr8_2:46102244-46102263 Msa1250440:intron 30.0%
!!! CGTTTGTTTTTACAAAAGTG+CGG + chr8_2:46102842-46102861 None:intergenic 30.0%
ATGGTTGTATAATTTCTCTC+TGG - chr8_2:46102904-46102923 Msa1250440:CDS 30.0%
!! ATTGTTTTACAGATTGGTGA+AGG - chr8_2:46102963-46102982 Msa1250440:CDS 30.0%
ATTGAATGTTTCGCTTAGAG+TGG + chr8_2:46100186-46100205 None:intergenic 35.0%
ACTCTAAGCGAAACATTCAA+TGG - chr8_2:46100185-46100204 Msa1250440:intron 35.0%
! AATCTGAGATGACTGAAAAG+AGG + chr8_2:46100330-46100349 None:intergenic 35.0%
CGTATAAATCTGTTGGGTTT+GGG + chr8_2:46100367-46100386 None:intergenic 35.0%
CCAAACCCAACAGATTTATA+CGG - chr8_2:46100365-46100384 Msa1250440:intron 35.0%
CAAACCCAACAGATTTATAC+GGG - chr8_2:46100366-46100385 Msa1250440:intron 35.0%
CAAACCCGTATAAATCTGTT+GGG + chr8_2:46100373-46100392 None:intergenic 35.0%
TCAAACCCGTATAAATCTGT+TGG + chr8_2:46100374-46100393 None:intergenic 35.0%
ATCACAATCTAAGAGGTCTT+CGG - chr8_2:46100505-46100524 Msa1250440:intron 35.0%
AGAAAACGAAGAGAGAAACT+CGG - chr8_2:46100557-46100576 Msa1250440:intron 35.0%
GACTAGTAAGACATAGTCAT+AGG + chr8_2:46100984-46101003 None:intergenic 35.0%
AGAAAAAGGGAAGAACACAA+AGG - chr8_2:46101103-46101122 Msa1250440:intron 35.0%
CACTTTCAGTTTCACAATAG+TGG + chr8_2:46101163-46101182 None:intergenic 35.0%
TACAATCATTGCTGAGGTAA+AGG - chr8_2:46101361-46101380 Msa1250440:intron 35.0%
! TAGAGAGAGAAGTATGAACA+TGG - chr8_2:46101665-46101684 Msa1250440:intron 35.0%
! AGAGAGAGAAGTATGAACAT+GGG - chr8_2:46101666-46101685 Msa1250440:intron 35.0%
GGCAACACAATATTTGAAAG+GGG + chr8_2:46101817-46101836 None:intergenic 35.0%
GGGCAACACAATATTTGAAA+GGG + chr8_2:46101818-46101837 None:intergenic 35.0%
! TTCATAAGTTGAGATTGTGG+GGG + chr8_2:46101838-46101857 None:intergenic 35.0%
TGACAAAGAGAATAATGGCA+TGG + chr8_2:46102114-46102133 None:intergenic 35.0%
TCCAAACATGTCATTAGTGA+CGG + chr8_2:46102723-46102742 None:intergenic 35.0%
ATGCATTGGGAAGTGAAATT+TGG + chr8_2:46102760-46102779 None:intergenic 35.0%
TATCACGTCTAACATGCATT+GGG + chr8_2:46102773-46102792 None:intergenic 35.0%
ATATCACGTCTAACATGCAT+TGG + chr8_2:46102774-46102793 None:intergenic 35.0%
! ATTCTGGACGAGTTCTTTTA+AGG + chr8_2:46103000-46103019 None:intergenic 35.0%
TCTTTGTTCCTCAAAGGCAA+TGG + chr8_2:46100138-46100157 None:intergenic 40.0%
AGTCTCTCTTTGTTCCTCAA+AGG + chr8_2:46100144-46100163 None:intergenic 40.0%
TGAGATGACTGAAAAGAGGA+TGG + chr8_2:46100326-46100345 None:intergenic 40.0%
CCGTATAAATCTGTTGGGTT+TGG + chr8_2:46100368-46100387 None:intergenic 40.0%
! TTCAGTGTCATTGCCCAATT+TGG - chr8_2:46100415-46100434 Msa1250440:intron 40.0%
ACTCATTCCTGACCTAAAGA+AGG - chr8_2:46100604-46100623 Msa1250440:intron 40.0%
CTTCTTACCTTCTTTAGGTC+AGG + chr8_2:46100614-46100633 None:intergenic 40.0%
CTTTAGATCGAAGACATACC+AGG - chr8_2:46100893-46100912 Msa1250440:intron 40.0%
AACGCTCTTTAAGCTCTTTG+AGG + chr8_2:46101067-46101086 None:intergenic 40.0%
CACAAAGGAAGATCAATCGA+TGG - chr8_2:46101118-46101137 Msa1250440:intron 40.0%
AAAGGAAGATCAATCGATGG+TGG - chr8_2:46101121-46101140 Msa1250440:intron 40.0%
! ACAATAGTGGTCTGGTTTGT+TGG + chr8_2:46101150-46101169 None:intergenic 40.0%
TCAGTTTCACAATAGTGGTC+TGG + chr8_2:46101158-46101177 None:intergenic 40.0%
!! AGGGTAGAAGCAAAAGTGTT+AGG - chr8_2:46101203-46101222 Msa1250440:intron 40.0%
!! GGGTAGAAGCAAAAGTGTTA+GGG - chr8_2:46101204-46101223 Msa1250440:intron 40.0%
! CAAGAAGTCCATCATGCTTT+TGG + chr8_2:46101256-46101275 None:intergenic 40.0%
CAGCAATGATTGTAATGTCG+TGG + chr8_2:46101355-46101374 None:intergenic 40.0%
CGACATTACAATCATTGCTG+AGG - chr8_2:46101355-46101374 Msa1250440:intron 40.0%
ATCTGCTATAGTGACTGTGA+CGG + chr8_2:46101789-46101808 None:intergenic 40.0%
GGGGCAACACAATATTTGAA+AGG + chr8_2:46101819-46101838 None:intergenic 40.0%
!! ATTTTGGGTGTGGTAGTATG+TGG + chr8_2:46102172-46102191 None:intergenic 40.0%
!! TGCAGTTTGTATTTTGGGTG+TGG + chr8_2:46102182-46102201 None:intergenic 40.0%
CCAAACATGTCATTAGTGAC+GGG + chr8_2:46102722-46102741 None:intergenic 40.0%
! ATGTAGCTTTTGCTTTGCCA+TGG - chr8_2:46102809-46102828 Msa1250440:CDS 40.0%
! TGTAGCTTTTGCTTTGCCAT+GGG - chr8_2:46102810-46102829 Msa1250440:CDS 40.0%
AGAGTTTCTCTCACGTGACA+TGG - chr8_2:46100106-46100125 Msa1250440:CDS 45.0%
GAATGTTTCGCTTAGAGTGG+TGG + chr8_2:46100183-46100202 None:intergenic 45.0%
CTGAAAAGAGGATGGAGATG+CGG + chr8_2:46100318-46100337 None:intergenic 45.0%
!! GGGTTTGGGTTGTCAAAAGA+CGG + chr8_2:46100353-46100372 None:intergenic 45.0%
TTGGGCAATGACACTGAAGT+GGG + chr8_2:46100413-46100432 None:intergenic 45.0%
ATTGGGCAATGACACTGAAG+TGG + chr8_2:46100414-46100433 None:intergenic 45.0%
GAGTTCTGCAAGAGCAATGA+AGG + chr8_2:46100588-46100607 None:intergenic 45.0%
ATATATTCGACCGAGACACC+TGG + chr8_2:46100914-46100933 None:intergenic 45.0%
!! TTGGTGATGCTACTGCTATC+AGG - chr8_2:46101183-46101202 Msa1250440:intron 45.0%
! TGGTGATGCTACTGCTATCA+GGG - chr8_2:46101184-46101203 Msa1250440:intron 45.0%
! ATCCACTGCCAAAAGCATGA+TGG - chr8_2:46101245-46101264 Msa1250440:intron 45.0%
! GAGAGAAGTATGAACATGGG+CGG - chr8_2:46101669-46101688 Msa1250440:intron 45.0%
TCTGCTATAGTGACTGTGAC+GGG + chr8_2:46101788-46101807 None:intergenic 45.0%
AAGCTATAACCATGCCAGCT+CGG - chr8_2:46102057-46102076 Msa1250440:CDS 45.0%
!!! AAACGTTTTGGTTACCGAGC+TGG + chr8_2:46102074-46102093 None:intergenic 45.0%
CCCGTCACTAATGACATGTT+TGG - chr8_2:46102719-46102738 Msa1250440:CDS 45.0%
TGACATGTTTGGATCAGCGT+TGG - chr8_2:46102730-46102749 Msa1250440:CDS 45.0%
! AAAAGTGCGGTGAATTCCCA+TGG + chr8_2:46102829-46102848 None:intergenic 45.0%
! GAACTTGCTTGATGCTGACG+TGG - chr8_2:46100079-46100098 Msa1250440:CDS 50.0%
CGAAGACATACCAGGTGTCT+CGG - chr8_2:46100901-46100920 Msa1250440:intron 50.0%
! GTCCATCATGCTTTTGGCAG+TGG + chr8_2:46101250-46101269 None:intergenic 50.0%
GTCGTGGACAGAATCTCCAA+AGG + chr8_2:46101339-46101358 None:intergenic 50.0%
TATGAACATGGGCGGATCCA+CGG - chr8_2:46101677-46101696 Msa1250440:intron 50.0%
!! TTTTGGTTACCGAGCTGGCA+TGG + chr8_2:46102069-46102088 None:intergenic 50.0%
ACTCGTCCAGAATCTACGCA+TGG - chr8_2:46103007-46103026 Msa1250440:CDS 50.0%
ATGCTGCCATGCGTAGATTC+TGG + chr8_2:46103016-46103035 None:intergenic 50.0%
! GGCTAGTGCCATTGCCTTTG+AGG - chr8_2:46100127-46100146 Msa1250440:intron 55.0%
ATGAACATGGGCGGATCCAC+GGG - chr8_2:46101678-46101697 Msa1250440:intron 55.0%
GCGGATCCACGGGTTCAATT+GGG - chr8_2:46101688-46101707 Msa1250440:intron 55.0%
CGGATCCACGGGTTCAATTG+GGG - chr8_2:46101689-46101708 Msa1250440:intron 55.0%
GGCGGATCCACGGGTTCAAT+TGG - chr8_2:46101687-46101706 Msa1250440:intron 60.0%
GCTGTCCCCAATTGAACCCG+TGG + chr8_2:46101697-46101716 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 46100053 46103055 46100053 ID=Msa1250440;Name=Msa1250440
chr8_2 mRNA 46100053 46103055 46100053 ID=Msa1250440-mRNA-1;Parent=Msa1250440;Name=Msa1250440-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0|1|1|1|3|0|309
chr8_2 exon 46102558 46103055 46102558 ID=Msa1250440-mRNA-1:exon:13884;Parent=Msa1250440-mRNA-1
chr8_2 exon 46101732 46102082 46101732 ID=Msa1250440-mRNA-1:exon:13883;Parent=Msa1250440-mRNA-1
chr8_2 exon 46100053 46100133 46100053 ID=Msa1250440-mRNA-1:exon:13882;Parent=Msa1250440-mRNA-1
chr8_2 CDS 46102558 46103055 46102558 ID=Msa1250440-mRNA-1:cds;Parent=Msa1250440-mRNA-1
chr8_2 CDS 46101732 46102082 46101732 ID=Msa1250440-mRNA-1:cds;Parent=Msa1250440-mRNA-1
chr8_2 CDS 46100053 46100133 46100053 ID=Msa1250440-mRNA-1:cds;Parent=Msa1250440-mRNA-1
Gene Sequence

>Msa1250440

ATGTTATTTGACAAGGAATGCTACTTGAACTTGCTTGATGCTGACGTGGTAGAAGAGTTTCTCTCACGTGACATGGCTAGTGCCATTGCCTTTGAGGAACAAAGAGAGACTCTACAACAATGTATTTCCACCACTCTAAGCGAAACATTCAATGGTGAAACAAGTTTTGAGTCTTTTGACTTTGATTTTGAGAATCCAACCAAGAAGCTGAAATTTTTTGATCAAAGTAACAACATAACCAAAAGCTTTTCACAACAAATTTCCGCATCTCCATCCTCTTTTCAGTCATCTCAGATTCCGTCTTTTGACAACCCAAACCCAACAGATTTATACGGGTTTGATCAAAAACAGAAAGAACCCACTTCAGTGTCATTGCCCAATTTGGATAACACACGTTTTTCAGCACAACCCTCAATTGAGTCTTCAAAAAATCAGAACTTGGAAACTAAAACATCACAATCTAAGAGGTCTTCGGCTCATGTAAAAGATCACATTATGAAGGACAAGGCTTCAGTATTAGCAGATACAATCAAATACCTCAAAGAGCTTAAAGAGCGTTTGTCAATTTTAGAAGAAGAAAAAGGGAAGAACACAAAGGAAGATCAATCGATGGTGGTTTCCAACAAACCAGACCACTATTGTGAAACTGAAAGTGTTGGTGATGCTACTGCTATCAGGGTAGAAGCAAAAGTGTTAGGGAAAAAGATGTTGATTCGGATCCACTGCCAAAAGCATGATGGACTTCTTGTTAAAGTCATCACTGAGATTCAAAGTTTTCAACTATTAGTTGTCAATAACCGTATTTTAGCCTTTGGAGATTCTGTCCACGACATTACAATCATTGCTGAGATTGGTGAAGGCTACAACTTGACCTTAAAAGAACTCGTCCAGAATCTACGCATGGCAGCATTGAAGTTTATGTCATCGTAA
Protein sequence

>Msa1250440

MLFDKECYLNLLDADVVEEFLSRDMASAIAFEEQRETLQQCISTTLSETFNGETSFESFDFDFENPTKKLKFFDQSNNITKSFSQQISASPSSFQSSQIPSFDNPNPTDLYGFDQKQKEPTSVSLPNLDNTRFSAQPSIESSKNQNLETKTSQSKRSSAHVKDHIMKDKASVLADTIKYLKELKERLSILEEEKGKNTKEDQSMVVSNKPDHYCETESVGDATAIRVEAKVLGKKMLIRIHCQKHDGLLVKVITEIQSFQLLVVNNRILAFGDSVHDITIIAEIGEGYNLTLKELVQNLRMAALKFMSS*