AlfalfaGEDB | Msa1250630

M. sativa cultivar zhongmu-4 / Msa1250630

KEGG: -
GO: -
PFAM: PF00407.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1250630	XP_003628200.1	84.076	157	24	1	1	157	1	156	8.69e-93	276

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1250630	sp\|Q9SSK5\|MLP43_ARATH	34.000	150	96	3	3	150	7	155	6.64e-22	89.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1250630	G7L7T5	84.076	157	24	1	1	157	1	156	4.15e-93	276

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1228190	Msa1250630	0.800366	1.490381e-48	-8.615850e-47
Msa1242110	Msa1250630	0.951435	2.057288e-109	-8.615850e-47
Msa1250630	Msa1326700	0.974105	1.422458e-137	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1250630	MtrunA17_Chr8g0355721	84.076	157	24	1	1	157	1	156	7.99e-97	276
Msa1250630	MtrunA17_Chr8g0355661	80.255	157	30	1	1	157	1	156	4.64e-91	261
Msa1250630	MtrunA17_Chr8g0355741	76.433	157	37	0	1	157	1	157	3.77e-89	256
Msa1250630	MtrunA17_Chr8g0355811	70.701	157	45	1	1	157	1	156	2.90e-82	239
Msa1250630	MtrunA17_Chr8g0355781	72.368	152	42	0	1	152	1	152	4.24e-82	238
Msa1250630	MtrunA17_Chr8g0355641	69.231	156	48	0	1	156	1	156	8.10e-80	233
Msa1250630	MtrunA17_Chr8g0355761	71.711	152	43	0	1	152	1	152	1.61e-79	232
Msa1250630	MtrunA17_Chr8g0355821	69.032	155	47	1	1	155	1	154	3.72e-79	231
Msa1250630	MtrunA17_Chr8g0355801	68.153	157	49	1	1	157	1	156	1.46e-77	227
Msa1250630	MtrunA17_Chr8g0355791	68.790	157	48	1	1	157	1	156	3.32e-77	226

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1250630	AT5G28010.1	32.667	150	98	3	3	150	15	163	2.08e-23	90.5
Msa1250630	AT5G28010.2	32.667	150	98	3	3	150	15	163	2.08e-23	90.5
Msa1250630	AT1G70890.1	34.000	150	96	3	3	150	7	155	6.75e-23	89.0
Msa1250630	AT1G70840.1	31.333	150	100	3	3	150	20	168	1.02e-20	83.6
Msa1250630	AT1G70830.5	32.000	150	99	3	3	150	22	170	1.43e-20	83.6
Msa1250630	AT1G70870.1	42.708	96	54	1	56	151	44	138	1.99e-20	82.0
Msa1250630	AT1G70830.3	32.000	150	99	3	3	150	22	170	2.85e-20	83.2
Msa1250630	AT5G28000.1	30.667	150	101	3	3	150	13	161	9.34e-20	80.9
Msa1250630	AT1G70830.2	32.000	150	99	3	3	150	22	170	9.87e-20	82.8
Msa1250630	AT1G23120.1	29.167	144	97	3	7	150	7	145	1.20e-19	80.5

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Find 39 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACACTGGACTTATGTCAT+AGG	0.470664	8_2:-46368344	Msa1250630:intron
AAAGAGAAGAAAGAAAACTA+GGG	0.476000	8_2:+46368557	None:intergenic
AAATGTTCCATGTAGCCATT+TGG	0.413070	8_2:+46366366	None:intergenic
AACACTTATAAGCTCTTTGG+TGG	0.457999	8_2:-46366510	Msa1250630:CDS
AACTTCATGAAGGTGAAGAT+TGG	0.483425	8_2:-46368385	Msa1250630:CDS
AAGGTCATCCTTGAAGTAAT+TGG	0.283197	8_2:-46366459	Msa1250630:CDS
AATGGCTACATGGAACATTT+TGG	0.264766	8_2:-46366363	Msa1250630:CDS
AATTATTGTGATGTTTGTAA+TGG	0.494485	8_2:-46366184	Msa1250630:three_prime_UTR
ACTCTGATTTGGTTAAACAC+TGG	0.575483	8_2:-46368358	Msa1250630:CDS
AGATATTGATGAGCATTATA+GGG	0.551405	8_2:-46366487	Msa1250630:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAAGAGAAGAAAGAAAACT+AGG	+	chr8_2:46366247-46366266	None:intergenic	30.0%
AGATTGTAGAACTTATCAGA+GGG	+	chr8_2:46366339-46366358	None:intergenic	30.0%
TAGATTGTAGAACTTATCAG+AGG	+	chr8_2:46366340-46366359	None:intergenic	30.0%
CGACATAATAACATTTGCAA+AGG	-	chr8_2:46367096-46367115	Msa1250630:intron	30.0%
AGTCAACCTTATATCTATAG+AGG	+	chr8_2:46367377-46367396	None:intergenic	30.0%
CGAAAATAATCCTAGGTATA+AGG	-	chr8_2:46367442-46367461	Msa1250630:intron	30.0%
CTTCGTAAACTTATCTAAGT+TGG	-	chr8_2:46367650-46367669	Msa1250630:intron	30.0%
GAGATATTGATGAGCATTAT+AGG	-	chr8_2:46368312-46368331	Msa1250630:intron	30.0%
ATCCTTGAAGTAATTGGTAA+GGG	-	chr8_2:46368347-46368366	Msa1250630:CDS	30.0%
ATCTTCACCTTCATGAAGTT+TGG	+	chr8_2:46366415-46366434	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	46366178	46368644	46366178	ID=Msa1250630;Name=Msa1250630
chr8_2	mRNA	46366178	46368644	46366178	ID=Msa1250630-mRNA-1;Parent=Msa1250630;Name=Msa1250630-mRNA-1;_AED=0.30;_eAED=0.30;_QI=113\|1\|1\|1\|1\|1\|2\|127\|157
chr8_2	exon	46366178	46366591	46366178	ID=Msa1250630-mRNA-1:exon:13956;Parent=Msa1250630-mRNA-1
chr8_2	exon	46368345	46368644	46368345	ID=Msa1250630-mRNA-1:exon:13955;Parent=Msa1250630-mRNA-1
chr8_2	five_prime_UTR	46368532	46368644	46368532	ID=Msa1250630-mRNA-1:five_prime_utr;Parent=Msa1250630-mRNA-1
chr8_2	CDS	46368345	46368531	46368345	ID=Msa1250630-mRNA-1:cds;Parent=Msa1250630-mRNA-1
chr8_2	CDS	46366305	46366591	46366305	ID=Msa1250630-mRNA-1:cds;Parent=Msa1250630-mRNA-1
chr8_2	three_prime_UTR	46366178	46366304	46366178	ID=Msa1250630-mRNA-1:three_prime_utr;Parent=Msa1250630-mRNA-1

Gene Sequence

>Msa1250630

ATGGTGCTCTCTGGTAAGCTCAGTACTGAAATTGGAATCAAAACACCCTCTGATAAGTTCTACAATCTATTTGTAACAGAACTTCATGAAGTGCAAAACATTAGTGAAAGAGTTCACCATACCAAACTTCATGAAGGTGAAGATTGGCATCACTCTGATTTGGTTAAACACTGGACTTATGTCATAGATGGCAAGGTACACACATGTCATGAGAGTGTTGAAGAAGTTGATGAACTGAATAAAAAAAACACTTATAAGCTCTTTGGTGGAGATATTGATGAGCATTATAGGGTCTTTAAGGTCATCCTTGAAGTAATTGGTAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAAAAGATCAATGAGGATATTGACCCTCCAAATGGCTACATGGAACATTTTGGCAAAGTCACTAGAGATGTTGATGCTCATCTTGTCAAAGGCGCAAATATAGCTCTATAA

Protein sequence

>Msa1250630

MVLSGKLSTEIGIKTPSDKFYNLFVTELHEVQNISERVHHTKLHEGEDWHHSDLVKHWTYVIDGKVHTCHESVEEVDELNKKNTYKLFGGDIDEHYRVFKVILEVIGKGDGTAAAKWTVEYEKINEDIDPPNGYMEHFGKVTRDVDAHLVKGANIAL*