Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1253430 | XP_003629040.1 | 91.667 | 96 | 8 | 0 | 2 | 97 | 84 | 179 | 1.05e-51 | 170 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1253430 | G7L7W4 | 91.667 | 96 | 8 | 0 | 2 | 97 | 84 | 179 | 5.00e-52 | 170 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1253430 | Msa1253450 | 0.904398 | 1.279233e-79 | -8.615850e-47 |
| Msa1253430 | Msa1293750 | 0.841238 | 5.365376e-58 | -8.615850e-47 |
| Msa1200420 | Msa1253430 | 0.872573 | 2.866131e-67 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1253430 | MtrunA17_Chr8g0369901 | 91.667 | 96 | 8 | 0 | 2 | 97 | 84 | 179 | 9.64e-56 | 170 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCTTAAGTGGTTGAAATT+TGG | 0.225001 | 8_2:-50883268 | Msa1253430:CDS |
| CATTAAACTCACTATCTTGC+AGG | 0.316668 | 8_2:-50883336 | Msa1253430:CDS |
| ATTAGATGCAAATGAGAATA+TGG | 0.327895 | 8_2:-50883522 | Msa1253430:CDS |
| AATCAAAGCAATGTCTAGAA+TGG | 0.406480 | 8_2:-50883305 | Msa1253430:CDS |
| TGTCTAGAATGGGTTACAAT+AGG | 0.431528 | 8_2:-50883294 | Msa1253430:CDS |
| TGCAAATGAGAATATGGATA+TGG | 0.443801 | 8_2:-50883516 | Msa1253430:CDS |
| ACAATAGGAAATGGCTTAAG+TGG | 0.455244 | 8_2:-50883279 | Msa1253430:CDS |
| GAATGGGTTACAATAGGAAA+TGG | 0.469355 | 8_2:-50883288 | Msa1253430:CDS |
| TCTTCCACATCTATAATAGC+TGG | 0.475039 | 8_2:-50883569 | Msa1253430:CDS |
| TCTATAATAGCTGGAAAGCT+AGG | 0.479436 | 8_2:-50883560 | Msa1253430:CDS |
| ATCAAAGCAATGTCTAGAAT+GGG | 0.486283 | 8_2:-50883304 | Msa1253430:CDS |
| ATAGCTGGAAAGCTAGGAAA+AGG | 0.490776 | 8_2:-50883554 | Msa1253430:CDS |
| GTAGGATAGCATTGTTGTGA+AGG | 0.491797 | 8_2:-50883365 | Msa1253430:intron |
| TCTTCAGCTGAGTATTGACT+TGG | 0.499669 | 8_2:-50883618 | Msa1253430:CDS |
| GGAAAGCTAGGAAAAGGATC+AGG | 0.545629 | 8_2:-50883548 | Msa1253430:CDS |
| CTGAGTATTGACTTGGACAT+GGG | 0.547034 | 8_2:-50883611 | Msa1253430:CDS |
| CTTTCCAGCTATTATAGATG+TGG | 0.555271 | 8_2:+50883565 | None:intergenic |
| ATTAAACTCACTATCTTGCA+GGG | 0.576938 | 8_2:-50883335 | Msa1253430:CDS |
| AAAGTTACTTGTGTTATGAG+AGG | 0.587359 | 8_2:+50883467 | None:intergenic |
| GCTGAGTATTGACTTGGACA+TGG | 0.601595 | 8_2:-50883612 | Msa1253430:CDS |
| TGGACATGGGAAACACGTCA+AGG | 0.684180 | 8_2:-50883598 | Msa1253430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTTTTTCTTCTTCTAA+TGG | - | chr8_2:50883480-50883499 | Msa1253430:CDS | 15.0% |
| !! | AGAAGAAGAAAAAAATATGT+AGG | + | chr8_2:50883478-50883497 | None:intergenic | 20.0% |
| ! | ATTAGATGCAAATGAGAATA+TGG | - | chr8_2:50883362-50883381 | Msa1253430:CDS | 25.0% |
| TGCAAATGAGAATATGGATA+TGG | - | chr8_2:50883368-50883387 | Msa1253430:intron | 30.0% | |
| AAAGTTACTTGTGTTATGAG+AGG | + | chr8_2:50883420-50883439 | None:intergenic | 30.0% | |
| !! | TATGTAGGAAGTGTACTTTT+AGG | + | chr8_2:50883463-50883482 | None:intergenic | 30.0% |
| ATTAAACTCACTATCTTGCA+GGG | - | chr8_2:50883549-50883568 | Msa1253430:CDS | 30.0% | |
| AATCAAAGCAATGTCTAGAA+TGG | - | chr8_2:50883579-50883598 | Msa1253430:CDS | 30.0% | |
| ! | ATCAAAGCAATGTCTAGAAT+GGG | - | chr8_2:50883580-50883599 | Msa1253430:CDS | 30.0% |
| TCTTCCACATCTATAATAGC+TGG | - | chr8_2:50883315-50883334 | Msa1253430:CDS | 35.0% | |
| CTTTCCAGCTATTATAGATG+TGG | + | chr8_2:50883322-50883341 | None:intergenic | 35.0% | |
| TCTATAATAGCTGGAAAGCT+AGG | - | chr8_2:50883324-50883343 | Msa1253430:CDS | 35.0% | |
| CATTAAACTCACTATCTTGC+AGG | - | chr8_2:50883548-50883567 | Msa1253430:CDS | 35.0% | |
| !! | TGTCTAGAATGGGTTACAAT+AGG | - | chr8_2:50883590-50883609 | Msa1253430:CDS | 35.0% |
| !! | GAATGGGTTACAATAGGAAA+TGG | - | chr8_2:50883596-50883615 | Msa1253430:CDS | 35.0% |
| ACAATAGGAAATGGCTTAAG+TGG | - | chr8_2:50883605-50883624 | Msa1253430:CDS | 35.0% | |
| TGGCTTAAGTGGTTGAAATT+TGG | - | chr8_2:50883616-50883635 | Msa1253430:CDS | 35.0% | |
| !! | TCTTCAGCTGAGTATTGACT+TGG | - | chr8_2:50883266-50883285 | Msa1253430:CDS | 40.0% |
| !! | CTGAGTATTGACTTGGACAT+GGG | - | chr8_2:50883273-50883292 | Msa1253430:CDS | 40.0% |
| ATAGCTGGAAAGCTAGGAAA+AGG | - | chr8_2:50883330-50883349 | Msa1253430:CDS | 40.0% | |
| TCTTCTAATGGTCAGCTTTG+TGG | - | chr8_2:50883492-50883511 | Msa1253430:CDS | 40.0% | |
| GTAGGATAGCATTGTTGTGA+AGG | - | chr8_2:50883519-50883538 | Msa1253430:CDS | 40.0% | |
| !! | GCTGAGTATTGACTTGGACA+TGG | - | chr8_2:50883272-50883291 | Msa1253430:CDS | 45.0% |
| GGAAAGCTAGGAAAAGGATC+AGG | - | chr8_2:50883336-50883355 | Msa1253430:CDS | 45.0% | |
| TGGACATGGGAAACACGTCA+AGG | - | chr8_2:50883286-50883305 | Msa1253430:CDS | 50.0% | |
| !! | GGTCAGCTTTGTGGTCTTGT+AGG | - | chr8_2:50883501-50883520 | Msa1253430:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 50883261 | 50883645 | 50883261 | ID=Msa1253430;Name=Msa1253430 |
| chr8_2 | mRNA | 50883261 | 50883645 | 50883261 | ID=Msa1253430-mRNA-1;Parent=Msa1253430;Name=Msa1253430-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|97 |
| chr8_2 | exon | 50883475 | 50883645 | 50883475 | ID=Msa1253430-mRNA-1:exon:15573;Parent=Msa1253430-mRNA-1 |
| chr8_2 | exon | 50883261 | 50883383 | 50883261 | ID=Msa1253430-mRNA-1:exon:15572;Parent=Msa1253430-mRNA-1 |
| chr8_2 | CDS | 50883475 | 50883645 | 50883475 | ID=Msa1253430-mRNA-1:cds;Parent=Msa1253430-mRNA-1 |
| chr8_2 | CDS | 50883261 | 50883383 | 50883261 | ID=Msa1253430-mRNA-1:cds;Parent=Msa1253430-mRNA-1 |
| Gene Sequence |
| Protein sequence |
