AlfalfaGEDB | Msa1257370

M. sativa cultivar zhongmu-4 / Msa1257370

KEGG: K09422
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1257370	RHN63223.1	95.722	187	8	0	1	187	43	229	3.53e-129	372

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1257370	sp\|A0A4D6Q411\|MYB1_CROXC	62.687	134	47	1	4	134	3	136	9.11e-53	172

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1257370	A0A396IEI4	95.722	187	8	0	1	187	43	229	1.68e-129	372

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TFs/TRs:

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Gene ID	Type	Classification
Msa1257370	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0793060	Msa1257370	0.800788	1.223000e-48	-8.615850e-47
Msa1207030	Msa1257370	0.930497	1.503475e-93	-8.615850e-47
Msa1207120	Msa1257370	0.932828	4.734506e-95	-8.615850e-47
Msa1257370	Msa1319410	0.873853	1.065739e-67	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1257370	MtrunA17_Chr4g0055981	95.722	187	8	0	1	187	43	229	3.24e-133	372
Msa1257370	MtrunA17_Chr4g0070641	66.942	121	40	0	9	129	8	128	2.97e-55	173
Msa1257370	MtrunA17_Chr4g0070611	66.942	121	40	0	9	129	8	128	5.42e-55	177
Msa1257370	MtrunA17_Chr5g0435101	66.372	113	38	0	4	116	2	114	1.69e-51	165
Msa1257370	MtrunA17_Chr7g0275911	64.912	114	40	0	9	122	8	121	2.97e-51	166
Msa1257370	MtrunA17_Chr8g0346561	59.843	127	51	0	9	135	8	134	3.90e-51	166
Msa1257370	MtrunA17_Chr8g0343831	71.154	104	30	0	13	116	8	111	1.04e-50	164
Msa1257370	MtrunA17_Chr7g0240821	61.157	121	47	0	13	133	1	121	1.29e-49	160
Msa1257370	MtrunA17_Chr3g0121241	65.741	108	37	0	9	116	38	145	4.28e-49	161
Msa1257370	MtrunA17_Chr3g0131121	58.462	130	48	1	10	133	9	138	9.62e-49	160

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1257370	AT5G35550.1	62.308	130	49	0	10	139	11	140	3.07e-51	166
Msa1257370	AT5G14750.1	47.929	169	71	2	15	166	18	186	1.35e-49	160
Msa1257370	AT3G13540.1	62.712	118	44	0	9	126	19	136	2.56e-49	160
Msa1257370	AT1G22640.1	66.355	107	36	0	10	116	9	115	5.75e-49	160
Msa1257370	AT5G40330.1	53.103	145	54	2	15	145	14	158	9.80e-49	158
Msa1257370	AT1G35515.1	44.330	194	85	3	10	187	9	195	1.30e-48	157
Msa1257370	AT4G09460.1	60.684	117	46	0	10	126	9	125	1.84e-48	158
Msa1257370	AT4G38620.1	64.486	107	38	0	10	116	9	115	3.09e-48	159
Msa1257370	AT5G52600.1	57.692	130	55	0	10	139	9	138	3.35e-48	156
Msa1257370	AT2G16720.1	64.486	107	38	0	10	116	9	115	5.96e-48	158

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Find 55 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCGTTGCAAACAATTC+AGG	0.371468	8_2:+58008900	Msa1257370:CDS
AAACAATGAATAGAGGAGCA+TGG	0.625013	8_2:+58008817	Msa1257370:CDS
AAACCCATATGGTTCTTCAG+TGG	0.603396	8_2:-58009602	None:intergenic
AAATTCATGGTGCCAAGAAA+TGG	0.392964	8_2:+58008877	Msa1257370:CDS
AAGAACCATATGGGTTTGAT+TGG	0.366727	8_2:+58009608	Msa1257370:CDS
AAGAAGAGGATTTGATTATT+AGG	0.284473	8_2:+58009128	Msa1257370:CDS
AATGTTCCCTCTCTTGATAT+TGG	0.336344	8_2:-58009098	None:intergenic
ACAACGAAATTAAGAACTAT+TGG	0.381215	8_2:+58009398	Msa1257370:CDS
ACTAAGGAAACAATGAATAG+AGG	0.506733	8_2:+58008810	Msa1257370:CDS
AGAACCATATGGGTTTGATT+GGG	0.507623	8_2:+58009609	Msa1257370:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTATTCATTGTTTCCTTAGT+AGG	-	chr8_2:58008810-58008829	None:intergenic	30.0%
ACTAAGGAAACAATGAATAG+AGG	+	chr8_2:58008810-58008829	Msa1257370:CDS	30.0%
ATTACCTGAATTGTTTGCAA+CGG	-	chr8_2:58008907-58008926	None:intergenic	30.0%
ACAAGTTTAGAAAAGAAAGG+GGG	+	chr8_2:58008972-58008991	Msa1257370:intron	30.0%
GTTAACTTTCTTAGCATTTG+TGG	+	chr8_2:58009312-58009331	Msa1257370:intron	30.0%
GAGAATAAAGATTCAATTGG+TGG	+	chr8_2:58009529-58009548	Msa1257370:CDS	30.0%
TGATTGGGTCAATAAATTCT+TGG	+	chr8_2:58009624-58009643	Msa1257370:CDS	30.0%
TACATGACATTATACTTCAC+TGG	-	chr8_2:58009722-58009741	None:intergenic	30.0%
CTAATTCTAAATGCATGTTC+GGG	+	chr8_2:58009760-58009779	Msa1257370:three_prime_UTR	30.0%
CAGCTTCAACAAAATCATAT+TGG	+	chr8_2:58009839-58009858	Msa1257370:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	58008771	58009881	58008771	ID=Msa1257370;Name=Msa1257370
chr8_2	mRNA	58008771	58009881	58008771	ID=Msa1257370-mRNA-1;Parent=Msa1257370;Name=Msa1257370-mRNA-1;_AED=0.05;_eAED=0.05;_QI=15\|1\|1\|1\|1\|1\|3\|194\|197
chr8_2	exon	58008771	58008921	58008771	ID=Msa1257370-mRNA-1:exon:17893;Parent=Msa1257370-mRNA-1
chr8_2	exon	58009044	58009173	58009044	ID=Msa1257370-mRNA-1:exon:17894;Parent=Msa1257370-mRNA-1
chr8_2	exon	58009360	58009881	58009360	ID=Msa1257370-mRNA-1:exon:17895;Parent=Msa1257370-mRNA-1
chr8_2	five_prime_UTR	58008771	58008785	58008771	ID=Msa1257370-mRNA-1:five_prime_utr;Parent=Msa1257370-mRNA-1
chr8_2	CDS	58008786	58008921	58008786	ID=Msa1257370-mRNA-1:cds;Parent=Msa1257370-mRNA-1
chr8_2	CDS	58009044	58009173	58009044	ID=Msa1257370-mRNA-1:cds;Parent=Msa1257370-mRNA-1
chr8_2	CDS	58009360	58009687	58009360	ID=Msa1257370-mRNA-1:cds;Parent=Msa1257370-mRNA-1
chr8_2	three_prime_UTR	58009688	58009881	58009688	ID=Msa1257370-mRNA-1:three_prime_utr;Parent=Msa1257370-mRNA-1

Gene Sequence

>Msa1257370

ATGGCACCCAGAAAATATGGACCTACTAAGGAAACAATGAATAGAGGAGCATGGACCCAGGAGGAAGATCAAAAGCTAGCACAATGCATCCAAATTCATGGTGCCAAGAAATGGAAAACCGTTGCAAACAATTCAGGTTTGAATAGGTGTGGAAAGAGTTGCAGGCTAAGATGGTTGAACTATCTTAGACCCAATATCAAGAGAGGGAACATTTCAGATGAAGAAGAGGATTTGATTATTAGGCTTCACAAACTTCTTGGAAACAGGTGGTCTTTGATAGCTGGAAGACTTCCAGGGCGAACAGACAACGAAATTAAGAACTATTGGAATTCTCATTTGTGCAAAAAAGCCAGTCACATAGAGGAAAAACCAGAAACTTCCACTACACAAGAAACTATTGCCCAAGACAATGTTGTTGGGGATAATGCTATGTTAGAGAATAAAGATTCAATTGGTGGAAGTCTTGATTCAGATGTTATCATTGATGTGAATGAATTCTTAGACTTTTCCACTGAAGAACCATATGGGTTTGATTGGGTCAATAAATTCTTGGAAATTGATCGGTTTCACATTCCTGAGAACAGAAACATCTGA

Protein sequence

>Msa1257370

MAPRKYGPTKETMNRGAWTQEEDQKLAQCIQIHGAKKWKTVANNSGLNRCGKSCRLRWLNYLRPNIKRGNISDEEEDLIIRLHKLLGNRWSLIAGRLPGRTDNEIKNYWNSHLCKKASHIEEKPETSTTQETIAQDNVVGDNAMLENKDSIGGSLDSDVIIDVNEFLDFSTEEPYGFDWVNKFLEIDRFHIPENRNI*