AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1257810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1257810 MtrunA17_Chr4g0056431 98.675 302 4 0 1 302 1 302 0.0 623
Msa1257810 MtrunA17_Chr5g0399071 74.172 302 77 1 1 302 1 301 2.64e-157 451
Msa1257810 MtrunA17_Chr5g0399061 70.724 304 85 2 1 302 1 302 2.46e-155 446
Msa1257810 MtrunA17_Chr4g0063591 59.364 283 112 2 11 293 8 287 1.87e-96 295
Msa1257810 MtrunA17_Chr5g0439381 53.676 272 124 1 8 277 11 282 3.55e-82 259
Msa1257810 MtrunA17_Chr8g0375311 45.105 286 152 2 17 302 32 312 2.24e-80 254
Msa1257810 MtrunA17_Chr4g0005901 44.406 286 152 3 17 302 24 302 4.29e-79 250
Msa1257810 MtrunA17_Chr8g0375291 44.755 286 153 2 17 302 32 312 2.01e-78 249
Msa1257810 MtrunA17_Chr8g0375351 43.357 286 157 2 17 302 32 312 7.13e-77 245
Msa1257810 MtrunA17_Chr8g0375341 43.007 286 158 2 17 302 32 312 1.34e-76 244
Msa1257810 MtrunA17_Chr7g0241741 44.056 286 153 3 17 302 14 292 4.64e-75 240
Msa1257810 MtrunA17_Chr8g0375321 42.014 288 158 4 17 302 29 309 8.34e-75 239
Msa1257810 MtrunA17_Chr8g0375331 41.667 288 159 4 17 302 16 296 5.48e-74 237
Msa1257810 MtrunA17_Chr3g0104011 45.105 286 151 3 17 302 14 293 6.78e-74 237
Msa1257810 MtrunA17_Chr7g0261881 41.463 287 165 2 17 302 19 303 1.15e-72 234
Msa1257810 MtrunA17_Chr7g0261811 43.704 270 150 2 17 285 19 287 2.12e-72 234
Msa1257810 MtrunA17_Chr3g0142111 39.721 287 167 3 17 302 38 319 2.80e-70 221
Msa1257810 MtrunA17_Chr1g0166711 41.887 265 154 0 17 281 17 281 5.81e-70 227
Msa1257810 MtrunA17_Chr7g0261731 40.977 266 156 1 17 281 23 288 1.40e-69 219
Msa1257810 MtrunA17_Chr3g0142101 39.721 287 167 3 17 302 27 308 2.55e-68 223
Msa1257810 MtrunA17_Chr1g0166791 39.161 286 172 1 17 302 16 299 2.89e-67 220
Msa1257810 MtrunA17_Chr7g0261791 41.445 263 152 1 17 277 21 283 5.53e-67 219
Msa1257810 MtrunA17_Chr7g0261771 41.221 262 153 1 17 277 23 284 1.36e-66 218
Msa1257810 MtrunA17_Chr1g0187241 38.214 280 167 1 8 281 3 282 2.10e-66 218
Msa1257810 MtrunA17_Chr7g0261851 40.794 277 162 2 19 294 22 297 3.17e-66 217
Msa1257810 MtrunA17_Chr1g0166741 39.860 286 170 1 17 302 19 302 1.09e-65 216
Msa1257810 MtrunA17_Chr7g0261751 40.602 266 157 1 17 281 23 288 1.70e-65 214
Msa1257810 MtrunA17_Chr7g0255621 38.790 281 166 3 23 302 18 293 8.40e-65 214
Msa1257810 MtrunA17_Chr8g0375361 44.867 263 142 1 17 279 31 290 3.93e-64 212
Msa1257810 MtrunA17_Chr5g0422881 38.129 278 168 2 25 302 20 293 8.92e-64 211
Msa1257810 MtrunA17_Chr3g0142091 37.457 291 178 3 13 302 14 301 1.16e-63 210
Msa1257810 MtrunA17_Chr1g0166771 40.927 259 151 1 44 302 10 266 3.11e-61 203
Msa1257810 MtrunA17_Chr7g0214731 38.235 306 172 5 2 302 48 341 1.07e-58 198
Msa1257810 MtrunA17_Chr3g0114501 37.979 287 171 5 17 302 14 294 3.59e-58 196
Msa1257810 MtrunA17_Chr7g0255611 36.630 273 167 3 13 284 19 286 3.49e-57 193
Msa1257810 MtrunA17_Chr3g0112671 37.993 279 158 4 10 280 12 283 7.03e-57 192
Msa1257810 MtrunA17_Chr3g0133341 33.333 285 184 2 21 302 10 291 2.64e-56 191
Msa1257810 MtrunA17_Chr8g0379971 36.842 304 172 4 17 302 12 313 3.52e-56 191
Msa1257810 MtrunA17_Chr2g0284701 36.111 288 180 3 17 302 24 309 4.05e-56 191
Msa1257810 MtrunA17_Chr7g0217461 36.395 294 178 4 11 302 10 296 4.26e-56 190
Msa1257810 MtrunA17_Chr8g0362711 39.130 299 178 2 4 300 3 299 1.17e-54 186
Msa1257810 MtrunA17_Chr2g0276751 35.664 286 169 4 17 302 30 300 1.12e-53 184
Msa1257810 MtrunA17_Chr4g0008651 34.589 292 179 5 13 302 22 303 1.13e-53 184
Msa1257810 MtrunA17_Chr4g0014781 41.732 254 131 4 17 265 21 262 1.63e-53 183
Msa1257810 MtrunA17_Chr7g0255601 37.456 283 167 5 21 302 27 300 1.79e-53 184
Msa1257810 MtrunA17_Chr5g0415961 43.902 246 136 1 17 262 16 259 2.65e-53 183
Msa1257810 MtrunA17_Chr4g0014791 39.655 290 146 7 17 301 21 286 3.90e-53 182
Msa1257810 MtrunA17_Chr4g0014871 37.413 286 154 5 12 292 17 282 1.12e-52 181
Msa1257810 MtrunA17_Chr4g0014841 40.000 285 148 5 11 292 15 279 1.56e-52 180
Msa1257810 MtrunA17_Chr7g0240381 34.375 288 177 5 17 302 21 298 2.22e-51 177
Msa1257810 MtrunA17_Chr5g0400371 34.021 291 191 1 11 301 98 387 3.15e-51 179
Msa1257810 MtrunA17_Chr4g0014801 38.284 303 157 6 4 301 8 285 4.33e-51 176
Msa1257810 MtrunA17_Chr7g0214461 32.746 284 187 4 19 302 72 351 6.61e-50 174
Msa1257810 MtrunA17_Chr4g0054891 32.302 291 196 1 11 301 96 385 1.32e-49 174
Msa1257810 MtrunA17_Chr5g0415791 33.333 306 193 4 2 302 8 307 1.45e-49 173
Msa1257810 MtrunA17_Chr7g0271221 34.532 278 172 5 25 302 17 284 2.23e-49 172
Msa1257810 MtrunA17_Chr3g0112611 33.103 290 181 5 15 302 22 300 3.13e-49 172
Msa1257810 MtrunA17_Chr8g0379991 34.843 287 155 4 46 302 1 285 9.48e-47 165
Msa1257810 MtrunA17_Chr7g0218851 31.987 297 196 4 8 302 29 321 1.34e-46 165
Msa1257810 MtrunA17_Chr1g0193391 32.734 278 176 5 25 302 17 283 2.55e-46 164
Msa1257810 MtrunA17_Chr1g0213711 33.571 280 181 5 23 302 19 293 2.70e-46 164
Msa1257810 MtrunA17_Chr4g0059971 33.333 273 178 2 30 302 39 307 1.33e-45 162
Msa1257810 MtrunA17_Chr3g0077151 37.066 259 144 2 10 265 13 255 1.43e-45 161
Msa1257810 MtrunA17_Chr1g0166731 44.118 170 95 0 112 281 9 178 1.60e-44 157
Msa1257810 MtrunA17_Chr4g0011471 34.630 257 163 2 46 302 37 288 3.78e-43 155
Msa1257810 MtrunA17_Chr8g0362781 29.808 312 197 4 3 302 6 307 1.76e-42 154
Msa1257810 MtrunA17_Chr4g0059991 32.117 274 181 3 30 302 27 296 6.30e-42 152
Msa1257810 MtrunA17_Chr4g0059981 29.801 302 200 4 8 302 8 304 1.70e-41 151
Msa1257810 MtrunA17_Chr4g0011041 30.420 286 195 3 17 302 28 309 1.13e-40 149
Msa1257810 MtrunA17_Chr4g0060001 31.200 250 172 0 21 270 25 274 1.45e-40 149
Msa1257810 MtrunA17_Chr2g0331471 30.566 265 181 3 25 288 88 350 1.84e-39 146
Msa1257810 MtrunA17_Chr7g0240281 30.952 294 197 4 13 302 11 302 6.00e-39 144
Msa1257810 MtrunA17_Chr7g0240271 30.479 292 199 3 13 302 11 300 1.77e-37 140
Msa1257810 MtrunA17_Chr8g0353031 34.752 282 159 4 26 302 19 280 2.50e-37 139
Msa1257810 MtrunA17_Chr7g0261661 39.227 181 108 2 115 294 3 182 2.79e-37 138
Msa1257810 MtrunA17_Chr2g0331481 31.250 256 172 4 24 278 25 277 5.41e-37 139
Msa1257810 MtrunA17_Chr1g0157601 30.072 276 179 5 29 302 19 282 7.78e-35 132
Msa1257810 MtrunA17_Chr2g0325951 32.245 245 161 2 26 270 20 259 1.29e-34 132
Msa1257810 MtrunA17_Chr2g0326211 33.061 245 159 1 26 270 20 259 1.33e-34 131
Msa1257810 MtrunA17_Chr2g0331511 28.000 275 191 6 6 278 4 273 2.33e-34 131
Msa1257810 MtrunA17_Chr2g0331521 25.292 257 190 2 21 276 18 273 1.21e-33 129
Msa1257810 MtrunA17_Chr4g0069581 33.333 264 157 8 46 302 4 255 3.73e-33 127
Msa1257810 MtrunA17_Chr4g0003761 29.435 248 172 3 29 275 34 279 1.48e-32 126
Msa1257810 MtrunA17_Chr8g0390331 29.688 256 176 3 24 278 15 267 8.00e-32 124
Msa1257810 MtrunA17_Chr3g0112651 33.854 192 118 3 100 289 9 193 1.23e-31 117
Msa1257810 MtrunA17_Chr1g0157611 29.091 275 186 4 25 295 16 285 3.28e-30 119
Msa1257810 MtrunA17_Chr2g0325961 31.837 245 162 2 26 270 20 259 9.47e-30 118
Msa1257810 MtrunA17_Chr2g0331461 30.233 258 172 6 24 278 25 277 3.20e-29 117
Msa1257810 MtrunA17_Chr1g0147631 27.165 254 179 4 25 274 25 276 3.34e-28 114
Msa1257810 MtrunA17_Chr1g0177591 26.277 274 184 7 8 278 27 285 3.09e-27 111
Msa1257810 MtrunA17_Chr4g0060061 39.344 122 74 0 138 259 8 129 5.02e-27 102
Msa1257810 MtrunA17_Chr8g0362801 35.417 192 117 3 115 302 3 191 5.43e-27 109
Msa1257810 MtrunA17_Chr1g0157621 27.559 254 175 4 46 295 1 249 3.07e-26 108
Msa1257810 MtrunA17_Chr4g0014861 38.509 161 84 4 145 301 13 162 2.87e-23 99.0
Msa1257810 MtrunA17_Chr3g0082631 44.915 118 65 0 148 265 30 147 9.85e-23 97.8
Msa1257810 MtrunA17_Chr7g0240181 33.889 180 116 2 124 302 12 189 7.13e-22 95.5
Msa1257810 MtrunA17_Chr7g0240211 33.889 180 116 2 124 302 12 189 7.55e-22 95.1
Msa1257810 MtrunA17_Chr7g0235521 33.889 180 116 2 124 302 12 189 7.55e-22 95.1
Msa1257810 MtrunA17_Chr7g0240151 33.333 180 117 2 124 302 12 189 1.24e-21 94.4
Msa1257810 MtrunA17_Chr7g0240131 33.889 180 116 2 124 302 12 189 1.55e-21 94.4
Msa1257810 MtrunA17_Chr4g0014851 39.815 108 65 0 12 119 17 124 7.09e-21 87.4
Msa1257810 MtrunA17_Chr7g0261671 45.455 88 48 0 17 104 24 111 2.29e-20 84.7
Msa1257810 MtrunA17_Chr4g0060051 45.161 93 51 0 17 109 14 106 4.89e-20 84.0
Msa1257810 MtrunA17_Chr6g0451841 30.568 229 138 3 27 246 6 222 6.39e-20 89.7
Msa1257810 MtrunA17_Chr7g0234381 33.333 144 85 2 112 255 3 135 1.61e-19 88.2
Msa1257810 MtrunA17_Chr1g0166721 34.586 133 82 1 17 149 17 144 1.64e-19 83.2
Msa1257810 MtrunA17_Chr5g0415841 29.688 192 126 3 115 302 3 189 2.25e-19 87.8
Msa1257810 MtrunA17_Chr7g0234181 30.726 179 109 4 115 293 1 164 4.41e-19 86.7
Msa1257810 MtrunA17_Chr7g0234151 30.233 172 107 3 112 283 3 161 6.05e-19 86.7
Msa1257810 MtrunA17_Chr3g0112661 37.736 106 65 1 17 122 23 127 3.97e-17 76.6
Msa1257810 MtrunA17_Chr1g0177611 27.407 135 96 2 145 278 23 156 3.06e-12 66.6
Msa1257810 MtrunA17_Chr1g0177631 28.148 135 95 2 145 278 23 156 3.74e-12 66.6
Msa1257810 MtrunA17_Chr8g0362791 36.782 87 54 1 30 116 41 126 8.95e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1257810 AT1G12110.1 67.550 302 96 2 3 302 2 303 3.61e-139 405
Msa1257810 AT2G26690.1 56.507 292 117 4 11 302 8 289 2.18e-98 301
Msa1257810 AT3G21670.1 51.325 302 135 3 13 302 15 316 3.43e-88 275
Msa1257810 AT3G54140.1 45.965 285 150 2 17 301 14 294 1.17e-84 265
Msa1257810 AT3G54140.2 45.965 285 150 2 17 301 14 294 1.17e-84 265
Msa1257810 AT2G26690.2 56.061 264 106 4 39 302 3 256 6.23e-84 263
Msa1257810 AT2G26690.3 55.906 254 102 4 49 302 1 244 1.26e-78 248
Msa1257810 AT5G01180.2 42.756 283 155 4 17 299 15 290 3.06e-74 238
Msa1257810 AT5G01180.3 42.756 283 155 4 17 299 15 290 3.06e-74 238
Msa1257810 AT5G01180.1 42.756 283 155 4 17 299 15 290 3.06e-74 238
Msa1257810 AT2G02040.1 42.754 276 155 1 17 292 31 303 7.55e-74 237
Msa1257810 AT1G62200.1 41.786 280 158 3 17 294 42 318 2.22e-71 231
Msa1257810 AT1G62200.2 41.786 280 158 3 17 294 42 318 3.93e-71 231
Msa1257810 AT1G62200.3 41.786 280 158 3 17 294 56 332 4.75e-71 231
Msa1257810 AT5G46040.1 41.045 268 158 0 17 284 17 284 8.73e-71 229
Msa1257810 AT2G02020.2 41.219 279 158 2 17 295 32 304 4.17e-69 223
Msa1257810 AT2G02020.1 42.857 259 145 1 17 275 32 287 8.66e-69 223
Msa1257810 AT5G46050.1 39.630 270 163 0 17 286 17 286 6.65e-68 222
Msa1257810 AT1G59740.1 35.172 290 186 1 13 302 27 314 1.35e-61 205
Msa1257810 AT5G13400.1 36.770 291 176 4 17 301 53 341 1.41e-58 198
Msa1257810 AT1G68570.1 39.322 295 173 4 6 300 4 292 5.14e-57 193
Msa1257810 AT1G68570.2 41.538 260 148 2 41 300 7 262 2.40e-55 188
Msa1257810 AT2G37900.1 36.918 279 167 4 8 284 19 290 1.08e-54 187
Msa1257810 AT2G40460.1 36.934 287 174 4 17 302 14 294 9.75e-54 184
Msa1257810 AT3G53960.1 35.354 297 179 5 8 302 18 303 1.76e-53 184
Msa1257810 AT3G25280.1 37.226 274 147 4 17 289 11 260 7.39e-53 181
Msa1257810 AT1G69850.1 41.057 246 142 1 17 262 15 257 7.65e-52 179
Msa1257810 AT5G19640.1 34.672 274 172 3 28 301 60 326 8.58e-51 177
Msa1257810 AT1G22540.1 43.775 249 131 4 17 262 20 262 4.65e-50 174
Msa1257810 AT1G69870.1 33.929 280 179 2 25 302 49 324 7.50e-49 172
Msa1257810 AT4G21680.1 33.916 286 183 3 17 302 18 297 1.13e-48 171
Msa1257810 AT1G27040.1 40.244 246 147 0 17 262 17 262 1.81e-48 170
Msa1257810 AT1G27040.2 40.244 246 147 0 17 262 13 258 2.04e-48 170
Msa1257810 AT5G62730.1 35.556 270 150 4 6 267 31 284 5.54e-48 169
Msa1257810 AT1G22570.1 36.897 290 173 5 12 295 15 300 9.55e-48 168
Msa1257810 AT1G33440.1 36.585 287 173 4 17 302 28 306 1.78e-47 168
Msa1257810 AT3G25260.1 35.458 251 151 3 17 267 11 250 4.39e-47 165
Msa1257810 AT1G72125.1 34.694 294 174 4 7 295 9 289 3.17e-46 164
Msa1257810 AT1G72140.1 35.484 279 166 4 7 277 14 286 1.16e-45 162
Msa1257810 AT1G32450.1 34.118 255 167 1 17 271 27 280 6.05e-45 161
Msa1257810 AT1G22550.1 34.926 272 171 3 10 276 13 283 1.16e-44 160
Msa1257810 AT1G27040.3 41.518 224 131 0 39 262 7 230 7.50e-44 157
Msa1257810 AT5G62730.2 36.402 239 132 3 33 267 3 225 1.87e-43 156
Msa1257810 AT1G72120.1 34.545 275 164 4 1 268 1 266 2.35e-43 156
Msa1257810 AT5G46050.2 41.860 172 100 0 115 286 3 174 2.26e-42 152
Msa1257810 AT2G37900.2 38.967 213 121 4 74 284 6 211 9.10e-41 148
Msa1257810 AT3G54450.1 32.624 282 169 4 24 302 10 273 2.18e-40 148
Msa1257810 AT3G16180.1 31.102 254 171 3 21 271 16 268 6.80e-39 144
Msa1257810 AT5G28470.2 32.016 253 171 1 21 273 11 262 9.24e-39 144
Msa1257810 AT5G28470.1 32.016 253 171 1 21 273 11 262 9.43e-39 144
Msa1257810 AT3G45660.1 31.418 261 173 2 7 267 1 255 1.03e-38 143
Msa1257810 AT3G45660.3 31.418 261 173 2 7 267 1 255 1.03e-38 143
Msa1257810 AT3G45660.2 31.298 262 174 2 6 267 21 276 1.06e-38 144
Msa1257810 AT1G22570.2 36.364 264 158 5 38 295 4 263 1.40e-38 142
Msa1257810 AT1G72130.1 34.386 285 159 7 24 302 22 284 8.18e-38 140
Msa1257810 AT1G18880.1 31.273 275 179 5 30 302 22 288 8.26e-38 141
Msa1257810 AT1G69860.1 28.929 280 193 4 24 302 15 289 2.20e-37 140
Msa1257810 AT3G47960.1 34.167 240 156 1 30 269 64 301 2.06e-36 138
Msa1257810 AT5G62680.1 31.405 242 162 2 30 269 46 285 2.76e-36 137
Msa1257810 AT1G27080.1 30.389 283 184 3 25 302 12 286 1.38e-35 135
Msa1257810 AT1G52190.1 29.242 277 189 5 25 295 19 294 5.03e-35 134
Msa1257810 AT3G45710.1 31.020 245 161 3 22 266 18 254 6.01e-35 133
Msa1257810 AT3G45690.1 29.876 241 163 2 26 266 17 251 6.94e-35 132
Msa1257810 AT3G45680.1 29.960 247 165 3 20 266 15 253 1.42e-33 129
Msa1257810 AT3G45700.1 28.522 291 192 5 14 302 5 281 6.19e-33 127
Msa1257810 AT3G45650.1 27.347 245 172 2 23 267 18 256 2.57e-30 120
Msa1257810 AT3G45650.2 27.347 245 172 2 23 267 18 256 2.88e-30 120
Msa1257810 AT5G11570.1 29.577 213 142 4 37 246 23 230 1.11e-28 115
Msa1257810 AT5G14940.1 30.894 246 142 4 25 255 5 237 1.73e-27 112
Msa1257810 AT3G45720.1 28.070 228 154 3 35 262 31 248 3.81e-27 111
Msa1257810 AT1G27080.2 29.600 250 163 3 58 302 3 244 2.43e-26 108
Msa1257810 AT3G01350.1 28.287 251 148 3 24 255 4 241 2.48e-22 97.4
Msa1257810 AT1G72130.2 31.579 171 100 5 138 302 7 166 9.26e-15 74.7
Msa1257810 AT1G72130.3 31.579 171 100 5 138 302 7 166 9.26e-15 74.7

Find 74 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTTTGTGTGTTCTTATA+TGG 0.178369 8_2:+58751606 Msa1257810:CDS
TGCTTCAACTGTTGCAAATA+TGG 0.190845 8_2:-58750964 None:intergenic
TTGACTGGAACTATGCATTT+AGG 0.240557 8_2:+58750717 Msa1257810:CDS
CTTCGTTTGTTTCGTCGAAT+TGG 0.273393 8_2:-58751434 None:intergenic
AATGGGCATAGCTGTAAATT+TGG 0.280360 8_2:+58750686 Msa1257810:CDS
GAGTACTCTCCCTCAAATTC+AGG 0.283666 8_2:+58750422 Msa1257810:CDS
ACCTCCTCACTTCTTCCTAT+TGG 0.291828 8_2:+58750368 Msa1257810:five_prime_UTR
GTTGGAAGAGATTGGGGTTA+TGG 0.306784 8_2:+58751558 Msa1257810:CDS
TGATGTCCAACCACCAGTTT+TGG 0.310306 8_2:-58750496 None:intergenic
ACATCATCTGCAATGATCTT+AGG 0.331493 8_2:+58750513 Msa1257810:CDS
TTTGAGGGAGAGTACTCATT+TGG 0.350245 8_2:-58750416 None:intergenic
AAATCAAGTGTCTCAGGCTT+TGG 0.360766 8_2:+58751405 Msa1257810:CDS
ATATAAGTGGTGAAGTAAAA+TGG 0.368636 8_2:-58750393 None:intergenic
AAGACCATGTTGGAAGAGAT+TGG 0.374975 8_2:+58751550 Msa1257810:CDS
AGACCATGTTGGAAGAGATT+GGG 0.380571 8_2:+58751551 Msa1257810:CDS
ACATGGTTAAATTCTTCAAC+TGG 0.400044 8_2:+58751469 Msa1257810:CDS
ATTAGTTGAATGTTGTTTGC+TGG 0.402464 8_2:-58751333 None:intergenic
TTTAAAATCCTAGTCATCAA+TGG 0.415468 8_2:+58750289 Msa1257810:five_prime_UTR
TAACATAGTCACCAACTTCA+TGG 0.417110 8_2:+58750755 Msa1257810:CDS
AGCAAACAACATTCAACTAA+TGG 0.417456 8_2:+58751335 Msa1257810:CDS
GTGAGGAGGTATGGCACAAA+GGG 0.420597 8_2:-58750355 None:intergenic
TGCCACATAGCAAGCAGTTT+CGG 0.423688 8_2:+58751799 Msa1257810:CDS
TCTCTCTTTATATACAACTT+AGG 0.445373 8_2:-58750319 None:intergenic
TATCGGTTTAAGAAACTTGT+TGG 0.448144 8_2:+58751639 Msa1257810:CDS
CCTGCTGAAAGATCCAAAAC+TGG 0.463034 8_2:+58750483 Msa1257810:CDS
TGAAGTAAAATGGCACCAAT+AGG 0.464965 8_2:-58750383 None:intergenic
TGGTTTATGTGGCTGCTTGG+AGG 0.470365 8_2:+58751685 Msa1257810:CDS
ATGTGGCTGCTTGGAGGAAG+AGG 0.476181 8_2:+58751691 Msa1257810:CDS
CAGTGGTTTATGTGGCTGCT+TGG 0.479040 8_2:+58751682 Msa1257810:CDS
GGTTTGAAATCAAGTGTCTC+AGG 0.479380 8_2:+58751399 Msa1257810:CDS
TACGTGACTGCACTTGGAAT+AGG 0.480168 8_2:+58751375 Msa1257810:CDS
TGTGAGAGGTTAACAACAAT+GGG 0.485740 8_2:+58750669 Msa1257810:CDS
AATTTGGTGACATATTTGAC+TGG 0.493264 8_2:+58750702 Msa1257810:CDS
GCACTATACGTGACTGCACT+TGG 0.501447 8_2:+58751369 Msa1257810:CDS
TTTATGCTTTGTTTGCTTGG+TGG 0.503937 8_2:+58750786 Msa1257810:CDS
AACAACAACGAGGAATCATA+TGG 0.505294 8_2:-58751726 None:intergenic
GAAAGAGACCATTGATGACT+AGG 0.509440 8_2:-58750297 None:intergenic
AGTACTCTCCCTCAAATTCA+GGG 0.511632 8_2:+58750423 Msa1257810:CDS
TAGGAAGAAGTGAGGAGGTA+TGG 0.515564 8_2:-58750364 None:intergenic
GGCAAACAATTCAAGATGCA+TGG 0.519315 8_2:+58750445 Msa1257810:CDS
AAAACATGTTCCCATGAAGT+TGG 0.526746 8_2:-58750766 None:intergenic
ACCACTGCAATCTGAGTCAA+AGG 0.532078 8_2:-58751666 None:intergenic
AACATAGTCACCAACTTCAT+GGG 0.533077 8_2:+58750756 Msa1257810:CDS
TCAGATTGCAGTGGTTTATG+TGG 0.533701 8_2:+58751674 Msa1257810:CDS
TATATGGAACAAAGAGGTAT+CGG 0.534715 8_2:+58751622 Msa1257810:CDS
AACTGTTGCAAATATGGCTA+TGG 0.535861 8_2:-58750958 None:intergenic
TCAAGATGCATGGGACTGCA+AGG 0.541640 8_2:+58750455 Msa1257810:CDS
TACCGAAACTGCTTGCTATG+TGG 0.545893 8_2:-58751801 None:intergenic
TCCTTTGACTCAGATTGCAG+TGG 0.548723 8_2:+58751665 Msa1257810:CDS
TTGTTTGCCCCTGAATTTGA+GGG 0.550085 8_2:-58750431 None:intergenic
AGTACTCATTTGGATATAAG+TGG 0.552042 8_2:-58750406 None:intergenic
AAAGATCCAAAACTGGTGGT+TGG 0.557448 8_2:+58750490 Msa1257810:CDS
ATTGTTTGCCCCTGAATTTG+AGG 0.568720 8_2:-58750432 None:intergenic
ATGTGAGAGGTTAACAACAA+TGG 0.573976 8_2:+58750668 Msa1257810:CDS
GCAAACAATTCAAGATGCAT+GGG 0.583011 8_2:+58750446 Msa1257810:CDS
GACCATGTTGGAAGAGATTG+GGG 0.583524 8_2:+58751552 Msa1257810:CDS
AGTGAGGAGGTATGGCACAA+AGG 0.594327 8_2:-58750356 None:intergenic
CAGGAGTAGAAGCATGTGAG+AGG 0.601559 8_2:+58750655 Msa1257810:intron
GTGACTGCACTTGGAATAGG+TGG 0.605369 8_2:+58751378 Msa1257810:CDS
GTTTACATTCAAGACCATGT+TGG 0.621720 8_2:+58751540 Msa1257810:CDS
AACCCCAATCTCTTCCAACA+TGG 0.639041 8_2:-58751554 None:intergenic
AAACCTTCCAATAAAAGTGT+CGG 0.639102 8_2:-58750817 None:intergenic
CAAGATGCATGGGACTGCAA+GGG 0.643607 8_2:+58750456 Msa1257810:CDS
GTTGGTGACTATGTTAGCAG+AGG 0.651075 8_2:-58750748 None:intergenic
ACCAATAGGAAGAAGTGAGG+AGG 0.666026 8_2:-58750369 None:intergenic
TGTTCTTATATGGAACAAAG+AGG 0.675410 8_2:+58751616 Msa1257810:CDS
GCTGAAAGATCCAAAACTGG+TGG 0.682083 8_2:+58750486 Msa1257810:CDS
TGCTTGGAGGAAGAGGAACA+TGG 0.693498 8_2:+58751698 Msa1257810:CDS
GTACTCTCCCTCAAATTCAG+GGG 0.697575 8_2:+58750424 Msa1257810:CDS
GGCACCAATAGGAAGAAGTG+AGG 0.698436 8_2:-58750372 None:intergenic
TGAGGAGGTATGGCACAAAG+GGG 0.716938 8_2:-58750354 None:intergenic
ATATTGAAGATGAAATGTTG+AGG 0.721487 8_2:+58751760 Msa1257810:CDS
TTGCAAATATGGCTATGGTG+AGG 0.755146 8_2:-58750953 None:intergenic
ATCAACATTAAACAACAACG+AGG 0.808925 8_2:-58751736 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTCTAATTGTAT+AGG - chr8_2:58751007-58751026 None:intergenic 10.0%
!! TAATATAAAGAAAATGAAAA+GGG - chr8_2:58751178-58751197 None:intergenic 10.0%
!! ATAATATAAAGAAAATGAAA+AGG - chr8_2:58751179-58751198 None:intergenic 10.0%
!!! TTTATATTATATTTCACTTT+TGG + chr8_2:58751189-58751208 Msa1257810:intron 10.0%
!!! ATTTTTTATTCTAATTGTAT+AGG - chr8_2:58751007-58751026 None:intergenic 10.0%
!! TAATATAAAGAAAATGAAAA+GGG - chr8_2:58751178-58751197 None:intergenic 10.0%
!! ATAATATAAAGAAAATGAAA+AGG - chr8_2:58751179-58751198 None:intergenic 10.0%
!!! TTTATATTATATTTCACTTT+TGG + chr8_2:58751189-58751208 Msa1257810:intron 10.0%
!! TAATTTGATTGTAGAAAAAA+TGG - chr8_2:58751123-58751142 None:intergenic 15.0%
!! AATATAAAGAAAATGAAAAG+GGG - chr8_2:58751177-58751196 None:intergenic 15.0%
!! TAATTTGATTGTAGAAAAAA+TGG - chr8_2:58751123-58751142 None:intergenic 15.0%
!! AATATAAAGAAAATGAAAAG+GGG - chr8_2:58751177-58751196 None:intergenic 15.0%
!!! TAATTGTCTTGTTCAAATTT+TGG + chr8_2:58750593-58750612 Msa1257810:intron 20.0%
!!! ATTCATTTTCTTTCTAGTAT+TGG - chr8_2:58750851-58750870 None:intergenic 20.0%
!! TTTATTCTAATTGTATAGGT+AGG - chr8_2:58751003-58751022 None:intergenic 20.0%
!!! TAATTGTCTTGTTCAAATTT+TGG + chr8_2:58750593-58750612 Msa1257810:intron 20.0%
!!! ATTCATTTTCTTTCTAGTAT+TGG - chr8_2:58750851-58750870 None:intergenic 20.0%
!! TTTATTCTAATTGTATAGGT+AGG - chr8_2:58751003-58751022 None:intergenic 20.0%
!! TTTAAAATCCTAGTCATCAA+TGG + chr8_2:58750289-58750308 Msa1257810:five_prime_UTR 25.0%
! TCTCTCTTTATATACAACTT+AGG - chr8_2:58750322-58750341 None:intergenic 25.0%
! ATATAAGTGGTGAAGTAAAA+TGG - chr8_2:58750396-58750415 None:intergenic 25.0%
!!! TGTGATGGATAATATATTTC+AGG + chr8_2:58750636-58750655 Msa1257810:intron 25.0%
!! TAAGACAAATTATCGATTGT+CGG + chr8_2:58751094-58751113 Msa1257810:intron 25.0%
! TTATTTGATATGCACATACT+AGG + chr8_2:58751227-58751246 Msa1257810:intron 25.0%
! TATTTGATATGCACATACTA+GGG + chr8_2:58751228-58751247 Msa1257810:intron 25.0%
!!! TTCTTCTTTTTCATAAGCAT+AGG + chr8_2:58751492-58751511 Msa1257810:CDS 25.0%
! ATATTGAAGATGAAATGTTG+AGG + chr8_2:58751760-58751779 Msa1257810:CDS 25.0%
!! TTTAAAATCCTAGTCATCAA+TGG + chr8_2:58750289-58750308 Msa1257810:five_prime_UTR 25.0%
! TCTCTCTTTATATACAACTT+AGG - chr8_2:58750322-58750341 None:intergenic 25.0%
! ATATAAGTGGTGAAGTAAAA+TGG - chr8_2:58750396-58750415 None:intergenic 25.0%
!!! TGTGATGGATAATATATTTC+AGG + chr8_2:58750636-58750655 Msa1257810:intron 25.0%
!! TAAGACAAATTATCGATTGT+CGG + chr8_2:58751094-58751113 Msa1257810:intron 25.0%
! TTATTTGATATGCACATACT+AGG + chr8_2:58751227-58751246 Msa1257810:intron 25.0%
! TATTTGATATGCACATACTA+GGG + chr8_2:58751228-58751247 Msa1257810:intron 25.0%
!!! TTCTTCTTTTTCATAAGCAT+AGG + chr8_2:58751492-58751511 Msa1257810:CDS 25.0%
! ATATTGAAGATGAAATGTTG+AGG + chr8_2:58751760-58751779 Msa1257810:CDS 25.0%
AGTACTCATTTGGATATAAG+TGG - chr8_2:58750409-58750428 None:intergenic 30.0%
! AATTTGGTGACATATTTGAC+TGG + chr8_2:58750702-58750721 Msa1257810:CDS 30.0%
!!! TGTTTTATGCTTTGTTTGCT+TGG + chr8_2:58750783-58750802 Msa1257810:CDS 30.0%
! AAACCTTCCAATAAAAGTGT+CGG - chr8_2:58750820-58750839 None:intergenic 30.0%
!!! AGATTGCTTTTTGTGCATTT+TGG - chr8_2:58751300-58751319 None:intergenic 30.0%
! ATTAGTTGAATGTTGTTTGC+TGG - chr8_2:58751336-58751355 None:intergenic 30.0%
AGCAAACAACATTCAACTAA+TGG + chr8_2:58751335-58751354 Msa1257810:CDS 30.0%
CGAAGTTGAAAAAAATCACA+TGG + chr8_2:58751452-58751471 Msa1257810:CDS 30.0%
ACATGGTTAAATTCTTCAAC+TGG + chr8_2:58751469-58751488 Msa1257810:CDS 30.0%
TGTTCTTATATGGAACAAAG+AGG + chr8_2:58751616-58751635 Msa1257810:CDS 30.0%
! TATATGGAACAAAGAGGTAT+CGG + chr8_2:58751622-58751641 Msa1257810:CDS 30.0%
!! TATCGGTTTAAGAAACTTGT+TGG + chr8_2:58751639-58751658 Msa1257810:CDS 30.0%
ATCAACATTAAACAACAACG+AGG - chr8_2:58751739-58751758 None:intergenic 30.0%
AGTACTCATTTGGATATAAG+TGG - chr8_2:58750409-58750428 None:intergenic 30.0%
! AATTTGGTGACATATTTGAC+TGG + chr8_2:58750702-58750721 Msa1257810:CDS 30.0%
!!! TGTTTTATGCTTTGTTTGCT+TGG + chr8_2:58750783-58750802 Msa1257810:CDS 30.0%
! AAACCTTCCAATAAAAGTGT+CGG - chr8_2:58750820-58750839 None:intergenic 30.0%
!!! AGATTGCTTTTTGTGCATTT+TGG - chr8_2:58751300-58751319 None:intergenic 30.0%
! ATTAGTTGAATGTTGTTTGC+TGG - chr8_2:58751336-58751355 None:intergenic 30.0%
AGCAAACAACATTCAACTAA+TGG + chr8_2:58751335-58751354 Msa1257810:CDS 30.0%
CGAAGTTGAAAAAAATCACA+TGG + chr8_2:58751452-58751471 Msa1257810:CDS 30.0%
ACATGGTTAAATTCTTCAAC+TGG + chr8_2:58751469-58751488 Msa1257810:CDS 30.0%
TGTTCTTATATGGAACAAAG+AGG + chr8_2:58751616-58751635 Msa1257810:CDS 30.0%
! TATATGGAACAAAGAGGTAT+CGG + chr8_2:58751622-58751641 Msa1257810:CDS 30.0%
!! TATCGGTTTAAGAAACTTGT+TGG + chr8_2:58751639-58751658 Msa1257810:CDS 30.0%
ATCAACATTAAACAACAACG+AGG - chr8_2:58751739-58751758 None:intergenic 30.0%
TGAAGTAAAATGGCACCAAT+AGG - chr8_2:58750386-58750405 None:intergenic 35.0%
GCAAACAATTCAAGATGCAT+GGG + chr8_2:58750446-58750465 Msa1257810:CDS 35.0%
ACATCATCTGCAATGATCTT+AGG + chr8_2:58750513-58750532 Msa1257810:CDS 35.0%
!!! ACTAGTTTTATGCTGTGTGA+TGG + chr8_2:58750621-58750640 Msa1257810:intron 35.0%
ATGTGAGAGGTTAACAACAA+TGG + chr8_2:58750668-58750687 Msa1257810:CDS 35.0%
TGTGAGAGGTTAACAACAAT+GGG + chr8_2:58750669-58750688 Msa1257810:CDS 35.0%
AATGGGCATAGCTGTAAATT+TGG + chr8_2:58750686-58750705 Msa1257810:CDS 35.0%
TTGACTGGAACTATGCATTT+AGG + chr8_2:58750717-58750736 Msa1257810:CDS 35.0%
TAACATAGTCACCAACTTCA+TGG + chr8_2:58750755-58750774 Msa1257810:CDS 35.0%
AACATAGTCACCAACTTCAT+GGG + chr8_2:58750756-58750775 Msa1257810:CDS 35.0%
AAAACATGTTCCCATGAAGT+TGG - chr8_2:58750769-58750788 None:intergenic 35.0%
!! TTTATGCTTTGTTTGCTTGG+TGG + chr8_2:58750786-58750805 Msa1257810:CDS 35.0%
! TTTGTAGCCGACACTTTTAT+TGG + chr8_2:58750810-58750829 Msa1257810:CDS 35.0%
AACTGTTGCAAATATGGCTA+TGG - chr8_2:58750961-58750980 None:intergenic 35.0%
TGCTTCAACTGTTGCAAATA+TGG - chr8_2:58750967-58750986 None:intergenic 35.0%
!!! TTGCTTTTTGTGCATTTTGG+TGG - chr8_2:58751297-58751316 None:intergenic 35.0%
GTTTACATTCAAGACCATGT+TGG + chr8_2:58751540-58751559 Msa1257810:CDS 35.0%
GCTCTTTGTGTGTTCTTATA+TGG + chr8_2:58751606-58751625 Msa1257810:CDS 35.0%
AACAACAACGAGGAATCATA+TGG - chr8_2:58751729-58751748 None:intergenic 35.0%
TGAAGTAAAATGGCACCAAT+AGG - chr8_2:58750386-58750405 None:intergenic 35.0%
GCAAACAATTCAAGATGCAT+GGG + chr8_2:58750446-58750465 Msa1257810:CDS 35.0%
ACATCATCTGCAATGATCTT+AGG + chr8_2:58750513-58750532 Msa1257810:CDS 35.0%
!!! ACTAGTTTTATGCTGTGTGA+TGG + chr8_2:58750621-58750640 Msa1257810:intron 35.0%
ATGTGAGAGGTTAACAACAA+TGG + chr8_2:58750668-58750687 Msa1257810:CDS 35.0%
TGTGAGAGGTTAACAACAAT+GGG + chr8_2:58750669-58750688 Msa1257810:CDS 35.0%
AATGGGCATAGCTGTAAATT+TGG + chr8_2:58750686-58750705 Msa1257810:CDS 35.0%
TTGACTGGAACTATGCATTT+AGG + chr8_2:58750717-58750736 Msa1257810:CDS 35.0%
TAACATAGTCACCAACTTCA+TGG + chr8_2:58750755-58750774 Msa1257810:CDS 35.0%
AACATAGTCACCAACTTCAT+GGG + chr8_2:58750756-58750775 Msa1257810:CDS 35.0%
AAAACATGTTCCCATGAAGT+TGG - chr8_2:58750769-58750788 None:intergenic 35.0%
!! TTTATGCTTTGTTTGCTTGG+TGG + chr8_2:58750786-58750805 Msa1257810:CDS 35.0%
! TTTGTAGCCGACACTTTTAT+TGG + chr8_2:58750810-58750829 Msa1257810:CDS 35.0%
AACTGTTGCAAATATGGCTA+TGG - chr8_2:58750961-58750980 None:intergenic 35.0%
TGCTTCAACTGTTGCAAATA+TGG - chr8_2:58750967-58750986 None:intergenic 35.0%
!!! TTGCTTTTTGTGCATTTTGG+TGG - chr8_2:58751297-58751316 None:intergenic 35.0%
GTTTACATTCAAGACCATGT+TGG + chr8_2:58751540-58751559 Msa1257810:CDS 35.0%
GCTCTTTGTGTGTTCTTATA+TGG + chr8_2:58751606-58751625 Msa1257810:CDS 35.0%
AACAACAACGAGGAATCATA+TGG - chr8_2:58751729-58751748 None:intergenic 35.0%
! GAAAGAGACCATTGATGACT+AGG - chr8_2:58750300-58750319 None:intergenic 40.0%
!! TTTGAGGGAGAGTACTCATT+TGG - chr8_2:58750419-58750438 None:intergenic 40.0%
AGTACTCTCCCTCAAATTCA+GGG + chr8_2:58750423-58750442 Msa1257810:CDS 40.0%
TTGTTTGCCCCTGAATTTGA+GGG - chr8_2:58750434-58750453 None:intergenic 40.0%
ATTGTTTGCCCCTGAATTTG+AGG - chr8_2:58750435-58750454 None:intergenic 40.0%
GGCAAACAATTCAAGATGCA+TGG + chr8_2:58750445-58750464 Msa1257810:CDS 40.0%
!!! CAGTTTTGGATCTTTCAGCA+GGG - chr8_2:58750485-58750504 None:intergenic 40.0%
AAAGATCCAAAACTGGTGGT+TGG + chr8_2:58750490-58750509 Msa1257810:CDS 40.0%
! TAGCCGACACTTTTATTGGA+AGG + chr8_2:58750814-58750833 Msa1257810:CDS 40.0%
TTGCAAATATGGCTATGGTG+AGG - chr8_2:58750956-58750975 None:intergenic 40.0%
!! TTGTGCATTTTGGTGGATGT+AGG - chr8_2:58751290-58751309 None:intergenic 40.0%
! GGTTTGAAATCAAGTGTCTC+AGG + chr8_2:58751399-58751418 Msa1257810:CDS 40.0%
AAATCAAGTGTCTCAGGCTT+TGG + chr8_2:58751405-58751424 Msa1257810:CDS 40.0%
CTTCGTTTGTTTCGTCGAAT+TGG - chr8_2:58751437-58751456 None:intergenic 40.0%
AAGACCATGTTGGAAGAGAT+TGG + chr8_2:58751550-58751569 Msa1257810:CDS 40.0%
AGACCATGTTGGAAGAGATT+GGG + chr8_2:58751551-58751570 Msa1257810:CDS 40.0%
TCAGATTGCAGTGGTTTATG+TGG + chr8_2:58751674-58751693 Msa1257810:CDS 40.0%
! GAAAGAGACCATTGATGACT+AGG - chr8_2:58750300-58750319 None:intergenic 40.0%
!! TTTGAGGGAGAGTACTCATT+TGG - chr8_2:58750419-58750438 None:intergenic 40.0%
AGTACTCTCCCTCAAATTCA+GGG + chr8_2:58750423-58750442 Msa1257810:CDS 40.0%
TTGTTTGCCCCTGAATTTGA+GGG - chr8_2:58750434-58750453 None:intergenic 40.0%
ATTGTTTGCCCCTGAATTTG+AGG - chr8_2:58750435-58750454 None:intergenic 40.0%
GGCAAACAATTCAAGATGCA+TGG + chr8_2:58750445-58750464 Msa1257810:CDS 40.0%
!!! CAGTTTTGGATCTTTCAGCA+GGG - chr8_2:58750485-58750504 None:intergenic 40.0%
AAAGATCCAAAACTGGTGGT+TGG + chr8_2:58750490-58750509 Msa1257810:CDS 40.0%
! TAGCCGACACTTTTATTGGA+AGG + chr8_2:58750814-58750833 Msa1257810:CDS 40.0%
TTGCAAATATGGCTATGGTG+AGG - chr8_2:58750956-58750975 None:intergenic 40.0%
!! TTGTGCATTTTGGTGGATGT+AGG - chr8_2:58751290-58751309 None:intergenic 40.0%
! GGTTTGAAATCAAGTGTCTC+AGG + chr8_2:58751399-58751418 Msa1257810:CDS 40.0%
AAATCAAGTGTCTCAGGCTT+TGG + chr8_2:58751405-58751424 Msa1257810:CDS 40.0%
CTTCGTTTGTTTCGTCGAAT+TGG - chr8_2:58751437-58751456 None:intergenic 40.0%
AAGACCATGTTGGAAGAGAT+TGG + chr8_2:58751550-58751569 Msa1257810:CDS 40.0%
AGACCATGTTGGAAGAGATT+GGG + chr8_2:58751551-58751570 Msa1257810:CDS 40.0%
TCAGATTGCAGTGGTTTATG+TGG + chr8_2:58751674-58751693 Msa1257810:CDS 40.0%
TAGGAAGAAGTGAGGAGGTA+TGG - chr8_2:58750367-58750386 None:intergenic 45.0%
ACCTCCTCACTTCTTCCTAT+TGG + chr8_2:58750368-58750387 Msa1257810:five_prime_UTR 45.0%
ACCAATAGGAAGAAGTGAGG+AGG - chr8_2:58750372-58750391 None:intergenic 45.0%
GAGTACTCTCCCTCAAATTC+AGG + chr8_2:58750422-58750441 Msa1257810:CDS 45.0%
GTACTCTCCCTCAAATTCAG+GGG + chr8_2:58750424-58750443 Msa1257810:CDS 45.0%
!!! CCAGTTTTGGATCTTTCAGC+AGG - chr8_2:58750486-58750505 None:intergenic 45.0%
CCTGCTGAAAGATCCAAAAC+TGG + chr8_2:58750483-58750502 Msa1257810:CDS 45.0%
GCTGAAAGATCCAAAACTGG+TGG + chr8_2:58750486-58750505 Msa1257810:CDS 45.0%
! TGATGTCCAACCACCAGTTT+TGG - chr8_2:58750499-58750518 None:intergenic 45.0%
!! GTTGGTGACTATGTTAGCAG+AGG - chr8_2:58750751-58750770 None:intergenic 45.0%
! GGTGAGGTATCTGTCAAAGA+AGG - chr8_2:58750940-58750959 None:intergenic 45.0%
!! CATTTTGGTGGATGTAGGCT+TGG - chr8_2:58751285-58751304 None:intergenic 45.0%
TACGTGACTGCACTTGGAAT+AGG + chr8_2:58751375-58751394 Msa1257810:CDS 45.0%
GACCATGTTGGAAGAGATTG+GGG + chr8_2:58751552-58751571 Msa1257810:CDS 45.0%
AACCCCAATCTCTTCCAACA+TGG - chr8_2:58751557-58751576 None:intergenic 45.0%
GTTGGAAGAGATTGGGGTTA+TGG + chr8_2:58751558-58751577 Msa1257810:CDS 45.0%
TCCTTTGACTCAGATTGCAG+TGG + chr8_2:58751665-58751684 Msa1257810:CDS 45.0%
ACCACTGCAATCTGAGTCAA+AGG - chr8_2:58751669-58751688 None:intergenic 45.0%
TGCCACATAGCAAGCAGTTT+CGG + chr8_2:58751799-58751818 Msa1257810:CDS 45.0%
!! TACCGAAACTGCTTGCTATG+TGG - chr8_2:58751804-58751823 None:intergenic 45.0%
TAGGAAGAAGTGAGGAGGTA+TGG - chr8_2:58750367-58750386 None:intergenic 45.0%
ACCTCCTCACTTCTTCCTAT+TGG + chr8_2:58750368-58750387 Msa1257810:five_prime_UTR 45.0%
ACCAATAGGAAGAAGTGAGG+AGG - chr8_2:58750372-58750391 None:intergenic 45.0%
GAGTACTCTCCCTCAAATTC+AGG + chr8_2:58750422-58750441 Msa1257810:CDS 45.0%
GTACTCTCCCTCAAATTCAG+GGG + chr8_2:58750424-58750443 Msa1257810:CDS 45.0%
!!! CCAGTTTTGGATCTTTCAGC+AGG - chr8_2:58750486-58750505 None:intergenic 45.0%
CCTGCTGAAAGATCCAAAAC+TGG + chr8_2:58750483-58750502 Msa1257810:CDS 45.0%
GCTGAAAGATCCAAAACTGG+TGG + chr8_2:58750486-58750505 Msa1257810:CDS 45.0%
! TGATGTCCAACCACCAGTTT+TGG - chr8_2:58750499-58750518 None:intergenic 45.0%
!! GTTGGTGACTATGTTAGCAG+AGG - chr8_2:58750751-58750770 None:intergenic 45.0%
! GGTGAGGTATCTGTCAAAGA+AGG - chr8_2:58750940-58750959 None:intergenic 45.0%
!! CATTTTGGTGGATGTAGGCT+TGG - chr8_2:58751285-58751304 None:intergenic 45.0%
TACGTGACTGCACTTGGAAT+AGG + chr8_2:58751375-58751394 Msa1257810:CDS 45.0%
GACCATGTTGGAAGAGATTG+GGG + chr8_2:58751552-58751571 Msa1257810:CDS 45.0%
AACCCCAATCTCTTCCAACA+TGG - chr8_2:58751557-58751576 None:intergenic 45.0%
GTTGGAAGAGATTGGGGTTA+TGG + chr8_2:58751558-58751577 Msa1257810:CDS 45.0%
TCCTTTGACTCAGATTGCAG+TGG + chr8_2:58751665-58751684 Msa1257810:CDS 45.0%
ACCACTGCAATCTGAGTCAA+AGG - chr8_2:58751669-58751688 None:intergenic 45.0%
TGCCACATAGCAAGCAGTTT+CGG + chr8_2:58751799-58751818 Msa1257810:CDS 45.0%
!! TACCGAAACTGCTTGCTATG+TGG - chr8_2:58751804-58751823 None:intergenic 45.0%
! TGAGGAGGTATGGCACAAAG+GGG - chr8_2:58750357-58750376 None:intergenic 50.0%
! GTGAGGAGGTATGGCACAAA+GGG - chr8_2:58750358-58750377 None:intergenic 50.0%
! AGTGAGGAGGTATGGCACAA+AGG - chr8_2:58750359-58750378 None:intergenic 50.0%
GGCACCAATAGGAAGAAGTG+AGG - chr8_2:58750375-58750394 None:intergenic 50.0%
TCAAGATGCATGGGACTGCA+AGG + chr8_2:58750455-58750474 Msa1257810:CDS 50.0%
CAAGATGCATGGGACTGCAA+GGG + chr8_2:58750456-58750475 Msa1257810:CDS 50.0%
! CAGGAGTAGAAGCATGTGAG+AGG + chr8_2:58750655-58750674 Msa1257810:intron 50.0%
GCACTATACGTGACTGCACT+TGG + chr8_2:58751369-58751388 Msa1257810:CDS 50.0%
GTGACTGCACTTGGAATAGG+TGG + chr8_2:58751378-58751397 Msa1257810:CDS 50.0%
CAGTGGTTTATGTGGCTGCT+TGG + chr8_2:58751682-58751701 Msa1257810:CDS 50.0%
TGGTTTATGTGGCTGCTTGG+AGG + chr8_2:58751685-58751704 Msa1257810:CDS 50.0%
TGCTTGGAGGAAGAGGAACA+TGG + chr8_2:58751698-58751717 Msa1257810:CDS 50.0%
! TGAGGAGGTATGGCACAAAG+GGG - chr8_2:58750357-58750376 None:intergenic 50.0%
! GTGAGGAGGTATGGCACAAA+GGG - chr8_2:58750358-58750377 None:intergenic 50.0%
! AGTGAGGAGGTATGGCACAA+AGG - chr8_2:58750359-58750378 None:intergenic 50.0%
GGCACCAATAGGAAGAAGTG+AGG - chr8_2:58750375-58750394 None:intergenic 50.0%
TCAAGATGCATGGGACTGCA+AGG + chr8_2:58750455-58750474 Msa1257810:CDS 50.0%
CAAGATGCATGGGACTGCAA+GGG + chr8_2:58750456-58750475 Msa1257810:CDS 50.0%
! CAGGAGTAGAAGCATGTGAG+AGG + chr8_2:58750655-58750674 Msa1257810:intron 50.0%
GCACTATACGTGACTGCACT+TGG + chr8_2:58751369-58751388 Msa1257810:CDS 50.0%
GTGACTGCACTTGGAATAGG+TGG + chr8_2:58751378-58751397 Msa1257810:CDS 50.0%
CAGTGGTTTATGTGGCTGCT+TGG + chr8_2:58751682-58751701 Msa1257810:CDS 50.0%
TGGTTTATGTGGCTGCTTGG+AGG + chr8_2:58751685-58751704 Msa1257810:CDS 50.0%
TGCTTGGAGGAAGAGGAACA+TGG + chr8_2:58751698-58751717 Msa1257810:CDS 50.0%
ATGTGGCTGCTTGGAGGAAG+AGG + chr8_2:58751691-58751710 Msa1257810:CDS 55.0%
ATGTGGCTGCTTGGAGGAAG+AGG + chr8_2:58751691-58751710 Msa1257810:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 58750268 58751824 58750268 ID=Msa1257810;Name=Msa1257810
chr8_2 mRNA 58750268 58751824 58750268 ID=Msa1257810-mRNA-1;Parent=Msa1257810;Name=Msa1257810-mRNA-1;_AED=0.09;_eAED=0.09;_QI=152|1|0.75|1|1|1|4|0|302
chr8_2 exon 58750268 58750534 58750268 ID=Msa1257810-mRNA-1:exon:18190;Parent=Msa1257810-mRNA-1
chr8_2 exon 58750658 58750835 58750658 ID=Msa1257810-mRNA-1:exon:18191;Parent=Msa1257810-mRNA-1
chr8_2 exon 58750950 58750989 58750950 ID=Msa1257810-mRNA-1:exon:18192;Parent=Msa1257810-mRNA-1
chr8_2 exon 58751249 58751824 58751249 ID=Msa1257810-mRNA-1:exon:18193;Parent=Msa1257810-mRNA-1
chr8_2 five_prime_UTR 58750268 58750419 58750268 ID=Msa1257810-mRNA-1:five_prime_utr;Parent=Msa1257810-mRNA-1
chr8_2 CDS 58750420 58750534 58750420 ID=Msa1257810-mRNA-1:cds;Parent=Msa1257810-mRNA-1
chr8_2 CDS 58750658 58750835 58750658 ID=Msa1257810-mRNA-1:cds;Parent=Msa1257810-mRNA-1
chr8_2 CDS 58750950 58750989 58750950 ID=Msa1257810-mRNA-1:cds;Parent=Msa1257810-mRNA-1
chr8_2 CDS 58751249 58751824 58751249 ID=Msa1257810-mRNA-1:cds;Parent=Msa1257810-mRNA-1
Gene Sequence

>Msa1257810

ATGAGTACTCTCCCTCAAATTCAGGGGCAAACAATTCAAGATGCATGGGACTGCAAGGGTCACCCTGCTGAAAGATCCAAAACTGGTGGTTGGACATCATCTGCAATGATCTTAGGAGTAGAAGCATGTGAGAGGTTAACAACAATGGGCATAGCTGTAAATTTGGTGACATATTTGACTGGAACTATGCATTTAGGAAATGCTTCCTCTGCTAACATAGTCACCAACTTCATGGGAACATGTTTTATGCTTTGTTTGCTTGGTGGTTTTGTAGCCGACACTTTTATTGGAAGATACCTCACCATAGCCATATTTGCAACAGTTGAAGCAATTGGTGTTACAATCTTGACAATATCAACAATAATTCCAAGCCTACATCCACCAAAATGCACAAAAAGCAATCTCAAATCTTGTGAACCAGCAAACAACATTCAACTAATGGTTCTATACATAGCACTATACGTGACTGCACTTGGAATAGGTGGTTTGAAATCAAGTGTCTCAGGCTTTGGTTCAGACCAATTCGACGAAACAAACGAAGTTGAAAAAAATCACATGGTTAAATTCTTCAACTGGTTCTTCTTTTTCATAAGCATAGGTTCATTAACAGCAGTGACAATTCTTGTTTACATTCAAGACCATGTTGGAAGAGATTGGGGTTATGGAATATGTGTTTGTGCTATTGTTGTTGCTCTTTGTGTGTTCTTATATGGAACAAAGAGGTATCGGTTTAAGAAACTTGTTGGTAGTCCTTTGACTCAGATTGCAGTGGTTTATGTGGCTGCTTGGAGGAAGAGGAACATGGAACTGCCATATGATTCCTCGTTGTTGTTTAATGTTGATGATATTGAAGATGAAATGTTGAGGAAGAAGAAGCAAGTGTTGCCACATAGCAAGCAGTTTCGGTAA
Protein sequence

>Msa1257810

MSTLPQIQGQTIQDAWDCKGHPAERSKTGGWTSSAMILGVEACERLTTMGIAVNLVTYLTGTMHLGNASSANIVTNFMGTCFMLCLLGGFVADTFIGRYLTIAIFATVEAIGVTILTISTIIPSLHPPKCTKSNLKSCEPANNIQLMVLYIALYVTALGIGGLKSSVSGFGSDQFDETNEVEKNHMVKFFNWFFFFISIGSLTAVTILVYIQDHVGRDWGYGICVCAIVVALCVFLYGTKRYRFKKLVGSPLTQIAVVYVAAWRKRNMELPYDSSLLFNVDDIEDEMLRKKKQVLPHSKQFR*