Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1260150 | Msa1260160 | 0.882135 | 1.342654e-70 | -8.615850e-47 |
| Msa0985130 | Msa1260150 | 0.968717 | 4.480262e-129 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCCGCAGCCAATATCATT+TGG | 0.419971 | 8_2:-62033269 | Msa1260150:CDS |
| ATCAACAAAACATTGGCATT+GGG | 0.442427 | 8_2:+62033411 | None:intergenic |
| GGTACAGAGCCAAATGATAT+TGG | 0.445610 | 8_2:+62033260 | None:intergenic |
| TATCAACAAAACATTGGCAT+TGG | 0.453142 | 8_2:+62033410 | None:intergenic |
| GAGCCCTATGCTCATTGAAC+AGG | 0.459760 | 8_2:-62033220 | Msa1260150:intron |
| GACTTACTAATATGTCACTA+CGG | 0.503550 | 8_2:-62033446 | Msa1260150:CDS |
| TTACCTGTTCAATGAGCATA+GGG | 0.542489 | 8_2:+62033217 | None:intergenic |
| CCTAAATATCAACAAAACAT+TGG | 0.555904 | 8_2:+62033404 | None:intergenic |
| AGTGATGCATCAACTTATGA+TGG | 0.570183 | 8_2:+62033294 | None:intergenic |
| ACTTACTAATATGTCACTAC+GGG | 0.571008 | 8_2:-62033445 | Msa1260150:CDS |
| GTTACCTGTTCAATGAGCAT+AGG | 0.574967 | 8_2:+62033216 | None:intergenic |
| GAGCCAAATGATATTGGCTG+CGG | 0.587422 | 8_2:+62033266 | None:intergenic |
| TTGTTGATATTTAGGGACGG+AGG | 0.617463 | 8_2:-62033396 | Msa1260150:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATAATTAAATCTCATTTA+TGG | + | chr8_2:62033056-62033075 | None:intergenic | 15.0% |
| !!! | CAATGTTTTGTTGATATTTA+GGG | - | chr8_2:62032804-62032823 | Msa1260150:intron | 20.0% |
| !! | AATTATGATTATTCTAACAG+TGG | - | chr8_2:62033068-62033087 | Msa1260150:intron | 20.0% |
| !! | AAGAATAAAAATGTCAAAAG+AGG | + | chr8_2:62033393-62033412 | None:intergenic | 20.0% |
| ! | CCTAAATATCAACAAAACAT+TGG | + | chr8_2:62032806-62032825 | None:intergenic | 25.0% |
| !!! | CCAATGTTTTGTTGATATTT+AGG | - | chr8_2:62032803-62032822 | Msa1260150:intron | 25.0% |
| !!! | CCATAAAGTTTTGATTTTGA+AGG | + | chr8_2:62033016-62033035 | None:intergenic | 25.0% |
| ! | CCTTCAAAATCAAAACTTTA+TGG | - | chr8_2:62033013-62033032 | Msa1260150:intron | 25.0% |
| ! | TACATCAAATGTTGTGTATA+AGG | - | chr8_2:62033276-62033295 | Msa1260150:CDS | 25.0% |
| GACTTACTAATATGTCACTA+CGG | - | chr8_2:62032761-62032780 | Msa1260150:intron | 30.0% | |
| ACTTACTAATATGTCACTAC+GGG | - | chr8_2:62032762-62032781 | Msa1260150:intron | 30.0% | |
| ATCAACAAAACATTGGCATT+GGG | + | chr8_2:62032799-62032818 | None:intergenic | 30.0% | |
| TATCAACAAAACATTGGCAT+TGG | + | chr8_2:62032800-62032819 | None:intergenic | 30.0% | |
| !! | GTTTTGTTGATATTTAGGGA+CGG | - | chr8_2:62032808-62032827 | Msa1260150:intron | 30.0% |
| ! | CAGAAATATGTGAGTTGAAT+CGG | + | chr8_2:62033177-62033196 | None:intergenic | 30.0% |
| ATAGTTGCCTCATAACAAAA+CGG | + | chr8_2:62033223-62033242 | None:intergenic | 30.0% | |
| GATGTAGTATTCAGAAATTC+AGG | + | chr8_2:62033262-62033281 | None:intergenic | 30.0% | |
| !! | CAACTTATGATGGTTTTGCA+AGG | + | chr8_2:62032906-62032925 | None:intergenic | 35.0% |
| AGTGATGCATCAACTTATGA+TGG | + | chr8_2:62032916-62032935 | None:intergenic | 35.0% | |
| TTACCTGTTCAATGAGCATA+GGG | + | chr8_2:62032993-62033012 | None:intergenic | 35.0% | |
| CGATTCAACTCACATATTTC+TGG | - | chr8_2:62033175-62033194 | Msa1260150:intron | 35.0% | |
| TAGTTGCCTCATAACAAAAC+GGG | + | chr8_2:62033222-62033241 | None:intergenic | 35.0% | |
| AGGCAACTATGTTGTGTATA+AGG | - | chr8_2:62033233-62033252 | Msa1260150:CDS | 35.0% | |
| CAACTATGTTGTGTATAAGG+AGG | - | chr8_2:62033236-62033255 | Msa1260150:CDS | 35.0% | |
| GCAGGATTAACTACAGATTA+CGG | + | chr8_2:62033309-62033328 | None:intergenic | 35.0% | |
| ATGTCAAAGTTAGTATCTGC+AGG | + | chr8_2:62033426-62033445 | None:intergenic | 35.0% | |
| ! | GACATCTGATATGTTTCTGA+AGG | - | chr8_2:62033441-62033460 | Msa1260150:CDS | 35.0% |
| TTGTTGATATTTAGGGACGG+AGG | - | chr8_2:62032811-62032830 | Msa1260150:intron | 40.0% | |
| GGTACAGAGCCAAATGATAT+TGG | + | chr8_2:62032950-62032969 | None:intergenic | 40.0% | |
| !!! | CTCTGTACCGTACTCTTTTT+TGG | - | chr8_2:62032961-62032980 | Msa1260150:intron | 40.0% |
| !!! | TGTACCGTACTCTTTTTTGG+TGG | - | chr8_2:62032964-62032983 | Msa1260150:intron | 40.0% |
| !!! | GTACCGTACTCTTTTTTGGT+GGG | - | chr8_2:62032965-62032984 | Msa1260150:intron | 40.0% |
| GTTACCTGTTCAATGAGCAT+AGG | + | chr8_2:62032994-62033013 | None:intergenic | 40.0% | |
| ATGCTGTCCCAATTGTCTCA+AGG | - | chr8_2:62032836-62032855 | Msa1260150:intron | 45.0% | |
| TGCTGTCCCAATTGTCTCAA+GGG | - | chr8_2:62032837-62032856 | Msa1260150:intron | 45.0% | |
| GAAGAGCCCTTGAGACAATT+GGG | + | chr8_2:62032846-62032865 | None:intergenic | 45.0% | |
| TGAAGAGCCCTTGAGACAAT+TGG | + | chr8_2:62032847-62032866 | None:intergenic | 45.0% | |
| ! | GAGCCAAATGATATTGGCTG+CGG | + | chr8_2:62032944-62032963 | None:intergenic | 45.0% |
| GCTCCCACCAAAAAAGAGTA+CGG | + | chr8_2:62032971-62032990 | None:intergenic | 45.0% | |
| !! | TTGCGATGCTACTTTGTCAC+TGG | - | chr8_2:62033336-62033355 | Msa1260150:intron | 45.0% |
| !! | TGCGATGCTACTTTGTCACT+GGG | - | chr8_2:62033337-62033356 | Msa1260150:intron | 45.0% |
| !! | TGCTACTTTGTCACTGGGAT+AGG | - | chr8_2:62033342-62033361 | Msa1260150:intron | 45.0% |
| CTGCCGCAGCCAATATCATT+TGG | - | chr8_2:62032938-62032957 | Msa1260150:intron | 50.0% | |
| GAGCCCTATGCTCATTGAAC+AGG | - | chr8_2:62032987-62033006 | Msa1260150:intron | 50.0% | |
| ACAAGTGCAGAAGCAAGTGC+TGG | - | chr8_2:62033129-62033148 | Msa1260150:intron | 50.0% | |
| CAAGTGCAGAAGCAAGTGCT+GGG | - | chr8_2:62033130-62033149 | Msa1260150:intron | 50.0% | |
| !! | TCGACGCCCGTTTTGTTATG+AGG | - | chr8_2:62033213-62033232 | Msa1260150:intron | 50.0% |
| TAGCATCGCAATCAGACAGC+AGG | + | chr8_2:62033327-62033346 | None:intergenic | 50.0% | |
| ! | CTGATGGCGTGGGAACATCA+TGG | - | chr8_2:62033101-62033120 | Msa1260150:intron | 55.0% |
| CAGTGGTCGTCGCTCTCTGA+TGG | - | chr8_2:62033085-62033104 | Msa1260150:intron | 60.0% | |
| ! | TCGTCGCTCTCTGATGGCGT+GGG | - | chr8_2:62033091-62033110 | Msa1260150:intron | 60.0% |
| !! | TGCAGAAGCAAGTGCTGGGC+AGG | - | chr8_2:62033134-62033153 | Msa1260150:intron | 60.0% |
| !! | GCAGAAGCAAGTGCTGGGCA+GGG | - | chr8_2:62033135-62033154 | Msa1260150:intron | 60.0% |
| ! | GTCGTCGCTCTCTGATGGCG+TGG | - | chr8_2:62033090-62033109 | Msa1260150:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 62032745 | 62033484 | 62032745 | ID=Msa1260150;Name=Msa1260150 |
| chr8_2 | mRNA | 62032745 | 62033484 | 62032745 | ID=Msa1260150-mRNA-1;Parent=Msa1260150;Name=Msa1260150-mRNA-1;_AED=0.33;_eAED=0.72;_QI=0|0|0|0.66|0|0.33|3|0|73 |
| chr8_2 | exon | 62033397 | 62033484 | 62033397 | ID=Msa1260150-mRNA-1:exon:19684;Parent=Msa1260150-mRNA-1 |
| chr8_2 | exon | 62033221 | 62033347 | 62033221 | ID=Msa1260150-mRNA-1:exon:19683;Parent=Msa1260150-mRNA-1 |
| chr8_2 | exon | 62032745 | 62032748 | 62032745 | ID=Msa1260150-mRNA-1:exon:19682;Parent=Msa1260150-mRNA-1 |
| chr8_2 | CDS | 62033397 | 62033484 | 62033397 | ID=Msa1260150-mRNA-1:cds;Parent=Msa1260150-mRNA-1 |
| chr8_2 | CDS | 62033221 | 62033347 | 62033221 | ID=Msa1260150-mRNA-1:cds;Parent=Msa1260150-mRNA-1 |
| chr8_2 | CDS | 62032745 | 62032748 | 62032745 | ID=Msa1260150-mRNA-1:cds;Parent=Msa1260150-mRNA-1 |
| Gene Sequence |
| Protein sequence |
