Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0985130 | Msa0985140 | 0.809947 | 1.470829e-50 | -8.615850e-47 |
| Msa0985130 | Msa1260150 | 0.968717 | 4.480262e-129 | -8.615850e-47 |
| Msa0985130 | Msa1260160 | 0.866441 | 2.844194e-65 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCCGCAGCCAATATCATT+TGG | 0.419971 | 6_4:-31107393 | Msa0985130:CDS |
| ATCAACAAAACATTGGCATT+GGG | 0.442427 | 6_4:+31107535 | None:intergenic |
| GGTACAGAGCCAAATGATAT+TGG | 0.445610 | 6_4:+31107384 | None:intergenic |
| TATCAACAAAACATTGGCAT+TGG | 0.453142 | 6_4:+31107534 | None:intergenic |
| GAGCCCTATGCTCATTGAAC+AGG | 0.459760 | 6_4:-31107344 | Msa0985130:intron |
| GACTTACTAATATGTCACTA+CGG | 0.503550 | 6_4:-31107570 | Msa0985130:CDS |
| TTACCTGTTCAATGAGCATA+GGG | 0.542489 | 6_4:+31107341 | None:intergenic |
| CCTAAATATCAACAAAACAT+TGG | 0.555904 | 6_4:+31107528 | None:intergenic |
| AGTGATGCATCAACTTATGA+TGG | 0.570183 | 6_4:+31107418 | None:intergenic |
| ACTTACTAATATGTCACTAC+GGG | 0.571008 | 6_4:-31107569 | Msa0985130:CDS |
| GTTACCTGTTCAATGAGCAT+AGG | 0.574967 | 6_4:+31107340 | None:intergenic |
| GAGCCAAATGATATTGGCTG+CGG | 0.587422 | 6_4:+31107390 | None:intergenic |
| TTGTTGATATTTAGGGACGG+AGG | 0.617463 | 6_4:-31107520 | Msa0985130:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATAATTAAATCTCATTTA+TGG | + | chr6_4:31107180-31107199 | None:intergenic | 15.0% |
| !!! | CAATGTTTTGTTGATATTTA+GGG | - | chr6_4:31106928-31106947 | Msa0985130:intron | 20.0% |
| !! | AATTATGATTATTCTAACAG+TGG | - | chr6_4:31107192-31107211 | Msa0985130:intron | 20.0% |
| !! | AAGAATAAAAATGTCAAAAG+AGG | + | chr6_4:31107517-31107536 | None:intergenic | 20.0% |
| ! | CCTAAATATCAACAAAACAT+TGG | + | chr6_4:31106930-31106949 | None:intergenic | 25.0% |
| !!! | CCAATGTTTTGTTGATATTT+AGG | - | chr6_4:31106927-31106946 | Msa0985130:intron | 25.0% |
| !!! | CCATAAAGTTTTGATTTTGA+AGG | + | chr6_4:31107140-31107159 | None:intergenic | 25.0% |
| ! | CCTTCAAAATCAAAACTTTA+TGG | - | chr6_4:31107137-31107156 | Msa0985130:intron | 25.0% |
| ! | TACATCAAATGTTGTGTATA+AGG | - | chr6_4:31107400-31107419 | Msa0985130:CDS | 25.0% |
| GACTTACTAATATGTCACTA+CGG | - | chr6_4:31106885-31106904 | Msa0985130:intron | 30.0% | |
| ACTTACTAATATGTCACTAC+GGG | - | chr6_4:31106886-31106905 | Msa0985130:intron | 30.0% | |
| ATCAACAAAACATTGGCATT+GGG | + | chr6_4:31106923-31106942 | None:intergenic | 30.0% | |
| TATCAACAAAACATTGGCAT+TGG | + | chr6_4:31106924-31106943 | None:intergenic | 30.0% | |
| !! | GTTTTGTTGATATTTAGGGA+CGG | - | chr6_4:31106932-31106951 | Msa0985130:intron | 30.0% |
| ! | CAGAAATATGTGAGTTGAAT+CGG | + | chr6_4:31107301-31107320 | None:intergenic | 30.0% |
| ATAGTTGCCTCATAACAAAA+CGG | + | chr6_4:31107347-31107366 | None:intergenic | 30.0% | |
| GATGTAGTATTCAGAAATTC+AGG | + | chr6_4:31107386-31107405 | None:intergenic | 30.0% | |
| !! | CAACTTATGATGGTTTTGCA+AGG | + | chr6_4:31107030-31107049 | None:intergenic | 35.0% |
| AGTGATGCATCAACTTATGA+TGG | + | chr6_4:31107040-31107059 | None:intergenic | 35.0% | |
| TTACCTGTTCAATGAGCATA+GGG | + | chr6_4:31107117-31107136 | None:intergenic | 35.0% | |
| CGATTCAACTCACATATTTC+TGG | - | chr6_4:31107299-31107318 | Msa0985130:intron | 35.0% | |
| TAGTTGCCTCATAACAAAAC+GGG | + | chr6_4:31107346-31107365 | None:intergenic | 35.0% | |
| AGGCAACTATGTTGTGTATA+AGG | - | chr6_4:31107357-31107376 | Msa0985130:CDS | 35.0% | |
| CAACTATGTTGTGTATAAGG+AGG | - | chr6_4:31107360-31107379 | Msa0985130:CDS | 35.0% | |
| GCAGGATTAACTACAGATTA+CGG | + | chr6_4:31107433-31107452 | None:intergenic | 35.0% | |
| ATGTCAAAGTTAGTATCTGC+AGG | + | chr6_4:31107550-31107569 | None:intergenic | 35.0% | |
| ! | GACATCTGATATGTTTCTGA+AGG | - | chr6_4:31107565-31107584 | Msa0985130:CDS | 35.0% |
| TTGTTGATATTTAGGGACGG+AGG | - | chr6_4:31106935-31106954 | Msa0985130:intron | 40.0% | |
| GGTACAGAGCCAAATGATAT+TGG | + | chr6_4:31107074-31107093 | None:intergenic | 40.0% | |
| !!! | CTCTGTACCGTACTCTTTTT+TGG | - | chr6_4:31107085-31107104 | Msa0985130:intron | 40.0% |
| !!! | TGTACCGTACTCTTTTTTGG+TGG | - | chr6_4:31107088-31107107 | Msa0985130:intron | 40.0% |
| !!! | GTACCGTACTCTTTTTTGGT+GGG | - | chr6_4:31107089-31107108 | Msa0985130:intron | 40.0% |
| GTTACCTGTTCAATGAGCAT+AGG | + | chr6_4:31107118-31107137 | None:intergenic | 40.0% | |
| ATGCTGTCCCAATTGTCTCA+AGG | - | chr6_4:31106960-31106979 | Msa0985130:intron | 45.0% | |
| TGCTGTCCCAATTGTCTCAA+GGG | - | chr6_4:31106961-31106980 | Msa0985130:intron | 45.0% | |
| GAAGAGCCCTTGAGACAATT+GGG | + | chr6_4:31106970-31106989 | None:intergenic | 45.0% | |
| TGAAGAGCCCTTGAGACAAT+TGG | + | chr6_4:31106971-31106990 | None:intergenic | 45.0% | |
| ! | GAGCCAAATGATATTGGCTG+CGG | + | chr6_4:31107068-31107087 | None:intergenic | 45.0% |
| GCTCCCACCAAAAAAGAGTA+CGG | + | chr6_4:31107095-31107114 | None:intergenic | 45.0% | |
| !! | TTGCGATGCTACTTTGTCAC+TGG | - | chr6_4:31107460-31107479 | Msa0985130:intron | 45.0% |
| !! | TGCGATGCTACTTTGTCACT+GGG | - | chr6_4:31107461-31107480 | Msa0985130:intron | 45.0% |
| !! | TGCTACTTTGTCACTGGGAT+AGG | - | chr6_4:31107466-31107485 | Msa0985130:intron | 45.0% |
| CTGCCGCAGCCAATATCATT+TGG | - | chr6_4:31107062-31107081 | Msa0985130:intron | 50.0% | |
| GAGCCCTATGCTCATTGAAC+AGG | - | chr6_4:31107111-31107130 | Msa0985130:intron | 50.0% | |
| ACAAGTGCAGAAGCAAGTGC+TGG | - | chr6_4:31107253-31107272 | Msa0985130:intron | 50.0% | |
| CAAGTGCAGAAGCAAGTGCT+GGG | - | chr6_4:31107254-31107273 | Msa0985130:intron | 50.0% | |
| !! | TCGACGCCCGTTTTGTTATG+AGG | - | chr6_4:31107337-31107356 | Msa0985130:intron | 50.0% |
| TAGCATCGCAATCAGACAGC+AGG | + | chr6_4:31107451-31107470 | None:intergenic | 50.0% | |
| ! | CTGATGGCGTGGGAACATCA+TGG | - | chr6_4:31107225-31107244 | Msa0985130:intron | 55.0% |
| CAGTGGTCGTCGCTCTCTGA+TGG | - | chr6_4:31107209-31107228 | Msa0985130:intron | 60.0% | |
| ! | TCGTCGCTCTCTGATGGCGT+GGG | - | chr6_4:31107215-31107234 | Msa0985130:intron | 60.0% |
| !! | TGCAGAAGCAAGTGCTGGGC+AGG | - | chr6_4:31107258-31107277 | Msa0985130:intron | 60.0% |
| !! | GCAGAAGCAAGTGCTGGGCA+GGG | - | chr6_4:31107259-31107278 | Msa0985130:intron | 60.0% |
| ! | GTCGTCGCTCTCTGATGGCG+TGG | - | chr6_4:31107214-31107233 | Msa0985130:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 31106869 | 31107608 | 31106869 | ID=Msa0985130;Name=Msa0985130 |
| chr6_4 | mRNA | 31106869 | 31107608 | 31106869 | ID=Msa0985130-mRNA-1;Parent=Msa0985130;Name=Msa0985130-mRNA-1;_AED=0.33;_eAED=0.72;_QI=0|0|0|0.66|0|0|3|0|73 |
| chr6_4 | exon | 31107521 | 31107608 | 31107521 | ID=Msa0985130-mRNA-1:exon:8402;Parent=Msa0985130-mRNA-1 |
| chr6_4 | exon | 31107345 | 31107471 | 31107345 | ID=Msa0985130-mRNA-1:exon:8401;Parent=Msa0985130-mRNA-1 |
| chr6_4 | exon | 31106869 | 31106872 | 31106869 | ID=Msa0985130-mRNA-1:exon:8400;Parent=Msa0985130-mRNA-1 |
| chr6_4 | CDS | 31107521 | 31107608 | 31107521 | ID=Msa0985130-mRNA-1:cds;Parent=Msa0985130-mRNA-1 |
| chr6_4 | CDS | 31107345 | 31107471 | 31107345 | ID=Msa0985130-mRNA-1:cds;Parent=Msa0985130-mRNA-1 |
| chr6_4 | CDS | 31106869 | 31106872 | 31106869 | ID=Msa0985130-mRNA-1:cds;Parent=Msa0985130-mRNA-1 |
| Gene Sequence |
| Protein sequence |
