AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1266200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1266200 MtrunA17_Chr4g0070791 99.377 321 2 0 1 321 1 321 0.0 644
Msa1266200 MtrunA17_Chr5g0405851 29.237 236 152 3 35 270 229 449 5.83e-24 101
Msa1266200 MtrunA17_Chr5g0405851 32.593 135 90 1 164 298 259 392 2.36e-14 73.6
Msa1266200 MtrunA17_Chr5g0405851 25.217 230 150 5 90 318 241 449 6.90e-13 68.9
Msa1266200 MtrunA17_Chr1g0172501 30.252 238 151 3 33 270 330 552 2.34e-23 100
Msa1266200 MtrunA17_Chr1g0172501 31.469 143 97 1 156 298 354 495 1.80e-12 67.8
Msa1266200 MtrunA17_Chr1g0172501 29.577 142 99 1 156 297 270 410 3.29e-12 67.0
Msa1266200 MtrunA17_Chr3g0093681 29.832 238 152 3 33 270 330 552 1.64e-22 97.8
Msa1266200 MtrunA17_Chr3g0093681 31.469 143 97 1 156 298 354 495 2.03e-12 67.8
Msa1266200 MtrunA17_Chr3g0093681 29.577 142 99 1 156 297 270 410 3.06e-12 67.4
Msa1266200 MtrunA17_Chr1g0185501 25.468 267 182 3 32 295 41 293 1.40e-21 93.2
Msa1266200 MtrunA17_Chr1g0185501 34.307 137 89 1 161 297 30 165 3.38e-17 80.9
Msa1266200 MtrunA17_Chr6g0448921 25.681 257 171 5 41 297 3 239 1.85e-21 95.1
Msa1266200 MtrunA17_Chr6g0448921 29.032 186 117 3 32 217 73 243 2.98e-14 73.6
Msa1266200 MtrunA17_Chr6g0448921 24.348 230 159 3 32 261 31 245 3.70e-13 70.1
Msa1266200 MtrunA17_Chr4g0067151 26.446 242 161 5 33 270 93 321 4.57e-20 89.0
Msa1266200 MtrunA17_Chr4g0067151 25.667 300 191 10 32 318 41 321 1.18e-19 87.8
Msa1266200 MtrunA17_Chr4g0067151 32.237 152 93 2 155 297 24 174 7.62e-19 85.5
Msa1266200 MtrunA17_Chr2g0281451 25.490 255 170 5 43 297 4 238 4.44e-19 88.2
Msa1266200 MtrunA17_Chr2g0281451 24.893 233 160 3 32 264 30 247 3.62e-15 76.6
Msa1266200 MtrunA17_Chr2g0281451 28.125 192 123 3 32 223 72 248 4.27e-15 76.3
Msa1266200 MtrunA17_Chr2g0281451 25.000 220 145 4 100 319 13 212 2.11e-12 68.2
Msa1266200 MtrunA17_Chr3g0136971 29.412 221 141 4 58 278 417 622 6.08e-19 87.4
Msa1266200 MtrunA17_Chr3g0136971 26.977 215 136 4 105 319 422 615 2.95e-13 70.5
Msa1266200 MtrunA17_Chr1g0176241 29.583 240 143 7 92 321 109 332 2.68e-18 85.1
Msa1266200 MtrunA17_Chr5g0401661 25.418 299 188 8 32 320 410 683 1.15e-17 83.6
Msa1266200 MtrunA17_Chr5g0401661 25.559 313 190 8 29 319 308 599 6.07e-17 81.6
Msa1266200 MtrunA17_Chr5g0401671 23.926 326 189 10 8 280 241 560 2.38e-17 82.8
Msa1266200 MtrunA17_Chr5g0401671 24.759 311 193 8 29 319 220 509 1.06e-16 80.9
Msa1266200 MtrunA17_Chr1g0200901 24.407 295 198 8 12 300 78 353 3.87e-17 81.6
Msa1266200 MtrunA17_Chr1g0200901 27.919 197 127 3 95 291 73 254 1.00e-12 68.2
Msa1266200 MtrunA17_Chr3g0103361 24.254 268 177 6 54 317 13 258 2.67e-16 78.2
Msa1266200 MtrunA17_Chr2g0295791 30.909 165 107 2 155 319 167 324 5.11e-16 78.6
Msa1266200 MtrunA17_Chr2g0295791 24.658 292 190 8 35 318 187 456 1.12e-12 68.6
Msa1266200 MtrunA17_Chr2g0295791 26.738 187 118 6 111 295 179 348 6.54e-12 66.2
Msa1266200 MtrunA17_Chr4g0053651 24.771 327 187 10 9 282 225 545 2.38e-15 76.6
Msa1266200 MtrunA17_Chr4g0053651 23.473 311 197 8 29 319 203 492 1.67e-12 68.2
Msa1266200 MtrunA17_Chr2g0331961 23.387 248 167 7 81 320 80 312 7.36e-15 73.9
Msa1266200 MtrunA17_Chr4g0059031 27.519 258 160 9 72 318 31 272 4.69e-14 73.2
Msa1266200 MtrunA17_Chr4g0059031 34.247 146 90 3 167 312 28 167 4.08e-13 70.1
Msa1266200 MtrunA17_Chr8g0390861 28.980 245 148 7 73 310 839 1064 5.14e-14 73.2
Msa1266200 MtrunA17_Chr1g0196201 32.624 141 92 2 161 298 88 228 9.34e-14 71.6
Msa1266200 MtrunA17_Chr7g0253861 23.197 319 203 8 12 317 53 342 1.34e-13 70.5
Msa1266200 MtrunA17_Chr7g0253861 24.638 207 134 4 95 297 48 236 1.91e-13 70.1
Msa1266200 MtrunA17_Chr8g0352821 22.945 292 206 4 6 296 217 490 1.36e-13 71.6
Msa1266200 MtrunA17_Chr2g0317491 27.232 224 137 8 105 321 568 772 4.88e-13 70.1
Msa1266200 MtrunA17_Chr4g0058091 25.188 266 168 8 33 288 245 489 5.80e-13 69.3
Msa1266200 MtrunA17_Chr8g0392701 29.348 184 120 5 11 189 146 324 6.20e-13 68.6
Msa1266200 MtrunA17_Chr8g0392701 31.973 147 95 3 155 297 60 205 1.67e-12 67.4
Msa1266200 MtrunA17_Chr8g0392701 25.385 260 157 7 34 272 79 322 1.33e-11 64.7
Msa1266200 MtrunA17_Chr2g0284241 26.014 296 172 9 32 284 22 313 9.82e-13 67.8
Msa1266200 MtrunA17_Chr4g0035611 25.843 267 166 7 31 295 64 300 1.48e-12 68.6
Msa1266200 MtrunA17_Chr3g0099061 30.159 189 112 5 109 296 221 390 1.75e-12 67.8
Msa1266200 MtrunA17_Chr1g0155601 27.778 324 192 15 12 318 61 359 4.65e-12 66.6
Msa1266200 MtrunA17_Chr3g0138311 27.869 244 149 11 31 270 676 896 8.14e-12 66.2
Msa1266200 MtrunA17_Chr2g0307941 24.904 261 180 7 13 271 61 307 1.22e-11 65.9
Msa1266200 MtrunA17_Chr8g0392731 27.854 219 133 8 109 321 66 265 2.46e-11 64.7
Msa1266200 MtrunA17_Chr3g0095501 23.588 301 187 10 8 272 264 557 3.17e-11 64.3
Msa1266200 MtrunA17_Chr3g0144511 23.492 315 179 11 11 270 62 369 4.28e-11 63.2
Msa1266200 MtrunA17_Chr4g0069701 23.636 275 184 7 56 319 435 694 5.46e-11 63.5
Msa1266200 MtrunA17_Chr2g0331981 23.596 267 185 6 31 293 28 279 5.78e-11 63.5
Msa1266200 MtrunA17_Chr4g0004241 25.346 217 143 6 81 293 78 279 5.80e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1266200 AT4G29830.1 78.816 321 68 0 1 321 1 321 0.0 522
Msa1266200 AT2G41500.1 31.933 238 147 3 33 270 313 535 1.29e-28 115
Msa1266200 AT2G41500.1 26.754 228 150 4 72 298 267 478 5.13e-13 70.1
Msa1266200 AT2G41500.2 31.933 238 147 3 33 270 313 535 1.29e-28 115
Msa1266200 AT2G41500.2 26.754 228 150 4 72 298 267 478 5.13e-13 70.1
Msa1266200 AT3G49660.1 28.631 241 157 4 32 270 85 312 3.46e-25 103
Msa1266200 AT3G49660.1 24.662 296 193 10 32 318 38 312 8.60e-19 85.9
Msa1266200 AT3G49660.1 33.099 142 89 2 161 297 27 167 9.03e-19 85.5
Msa1266200 AT5G52820.1 27.347 245 145 5 32 270 254 471 3.80e-19 88.2
Msa1266200 AT5G52820.1 30.808 198 117 6 92 284 99 281 1.18e-16 80.9
Msa1266200 AT5G52820.1 29.825 171 106 5 155 319 106 268 4.26e-13 70.1
Msa1266200 AT5G52820.1 22.699 326 212 8 11 305 148 464 1.81e-12 68.2
Msa1266200 AT1G71840.1 27.341 267 172 5 12 273 68 317 4.45e-19 87.4
Msa1266200 AT1G71840.1 27.500 200 126 4 87 284 56 238 3.51e-16 79.0
Msa1266200 AT5G23430.3 29.121 182 115 2 116 297 72 239 5.76e-19 88.2
Msa1266200 AT5G23430.3 24.783 230 158 3 32 261 31 245 9.09e-14 72.4
Msa1266200 AT5G23430.3 24.528 212 140 3 108 319 22 213 3.26e-13 70.9
Msa1266200 AT5G23430.2 29.121 182 115 2 116 297 72 239 5.76e-19 88.2
Msa1266200 AT5G23430.2 24.783 230 158 3 32 261 31 245 9.09e-14 72.4
Msa1266200 AT5G23430.2 24.528 212 140 3 108 319 22 213 3.26e-13 70.9
Msa1266200 AT5G23430.1 29.121 182 115 2 116 297 72 239 5.93e-19 88.2
Msa1266200 AT5G23430.1 24.783 230 158 3 32 261 31 245 9.10e-14 72.4
Msa1266200 AT5G23430.1 24.528 212 140 3 108 319 22 213 3.35e-13 70.9
Msa1266200 AT1G11160.1 26.482 253 168 4 12 264 13 247 1.03e-18 87.4
Msa1266200 AT1G11160.1 28.022 182 117 2 116 297 71 238 6.55e-15 76.3
Msa1266200 AT1G11160.1 27.604 192 124 4 32 223 72 248 2.11e-12 68.6
Msa1266200 AT1G11160.1 24.638 207 136 4 113 319 26 212 1.16e-11 66.2
Msa1266200 AT1G11160.4 26.482 253 168 4 12 264 13 247 1.10e-18 87.4
Msa1266200 AT1G11160.4 28.022 182 117 2 116 297 71 238 6.58e-15 75.9
Msa1266200 AT1G11160.4 27.604 192 124 4 32 223 72 248 2.16e-12 68.6
Msa1266200 AT1G11160.4 24.638 207 136 4 113 319 26 212 1.08e-11 66.2
Msa1266200 AT1G11160.2 26.482 253 168 4 12 264 13 247 1.19e-18 87.4
Msa1266200 AT1G11160.2 28.022 182 117 2 116 297 71 238 7.11e-15 75.9
Msa1266200 AT1G11160.2 27.604 192 124 4 32 223 72 248 2.47e-12 68.2
Msa1266200 AT1G11160.2 24.638 207 136 4 113 319 26 212 1.24e-11 66.2
Msa1266200 AT5G25150.2 29.767 215 136 4 58 272 416 615 1.57e-18 86.7
Msa1266200 AT5G25150.2 30.769 221 128 6 12 229 416 614 2.95e-15 77.0
Msa1266200 AT5G25150.2 25.581 215 139 4 105 319 421 614 1.00e-12 69.3
Msa1266200 AT5G25150.1 29.767 215 136 4 58 272 416 615 1.85e-18 86.7
Msa1266200 AT5G25150.1 30.769 221 128 6 12 229 416 614 2.88e-15 77.0
Msa1266200 AT5G25150.1 25.581 215 139 4 105 319 421 614 1.07e-12 69.3
Msa1266200 AT5G08390.1 25.292 257 172 5 41 297 3 239 2.10e-18 86.7
Msa1266200 AT5G08390.1 25.217 230 157 3 32 261 31 245 5.76e-16 79.3
Msa1266200 AT5G08390.1 25.000 212 139 3 108 319 22 213 1.10e-13 72.4
Msa1266200 AT4G02730.1 23.985 271 182 5 32 295 57 310 6.92e-18 83.6
Msa1266200 AT4G02730.1 28.485 165 110 3 156 319 41 198 3.88e-14 72.4
Msa1266200 AT4G02730.1 26.699 206 133 5 12 215 126 315 1.77e-11 64.7
Msa1266200 AT5G56130.1 25.614 285 181 8 38 317 1 259 8.86e-18 82.8
Msa1266200 AT5G67320.1 22.192 365 218 11 9 320 260 611 1.08e-17 84.3
Msa1266200 AT2G43770.1 27.053 207 133 4 93 297 44 234 9.23e-17 80.1
Msa1266200 AT2G43770.1 24.765 319 198 8 12 317 51 340 1.13e-16 80.1
Msa1266200 AT1G61210.3 25.692 253 170 4 12 264 13 247 1.09e-16 81.3
Msa1266200 AT1G61210.3 26.064 188 124 3 110 297 66 238 5.16e-14 73.6
Msa1266200 AT1G61210.3 24.590 183 124 2 113 295 26 194 3.24e-11 64.7
Msa1266200 AT1G61210.1 26.180 233 157 3 32 264 30 247 1.67e-16 80.9
Msa1266200 AT1G61210.1 26.064 188 124 3 110 297 66 238 6.63e-14 73.2
Msa1266200 AT1G61210.1 24.590 183 124 2 113 295 26 194 3.96e-11 64.7
Msa1266200 AT1G61210.2 26.180 233 157 3 32 264 30 247 1.78e-16 80.9
Msa1266200 AT1G61210.2 26.064 188 124 3 110 297 66 238 7.19e-14 72.8
Msa1266200 AT1G61210.2 24.590 183 124 2 113 295 26 194 3.96e-11 64.7
Msa1266200 AT5G13480.4 24.632 272 186 4 6 276 145 398 2.40e-16 80.1
Msa1266200 AT5G13480.1 24.632 272 186 4 6 276 157 410 2.56e-16 80.1
Msa1266200 AT5G13480.3 24.632 272 186 4 6 276 145 398 2.65e-16 80.1
Msa1266200 AT5G13480.2 24.632 272 186 4 6 276 168 421 2.96e-16 79.7
Msa1266200 AT1G11160.3 26.087 207 138 3 58 264 14 205 1.14e-15 78.2
Msa1266200 AT1G11160.3 28.022 182 117 2 116 297 29 196 5.85e-15 76.3
Msa1266200 AT1G11160.3 27.604 192 124 4 32 223 30 206 2.42e-12 68.2
Msa1266200 AT1G11160.3 24.841 157 111 2 163 319 21 170 2.37e-11 65.1
Msa1266200 AT2G47990.1 34.286 140 90 2 161 298 96 235 2.27e-15 77.0
Msa1266200 AT4G15900.1 30.303 165 108 2 155 319 173 330 4.75e-15 75.9
Msa1266200 AT4G15900.1 25.101 247 169 4 33 279 191 421 3.80e-13 70.1
Msa1266200 AT4G15900.1 25.121 207 130 7 110 314 184 367 4.89e-11 63.9
Msa1266200 AT3G15354.4 30.357 224 130 9 105 321 287 491 1.41e-13 71.6
Msa1266200 AT3G15354.5 30.357 224 130 9 105 321 287 491 1.41e-13 71.6
Msa1266200 AT2G22040.1 27.660 235 147 8 54 281 78 296 1.79e-13 70.5
Msa1266200 AT3G21540.1 23.881 335 219 10 1 321 384 696 2.00e-13 71.6
Msa1266200 AT3G15354.3 30.357 224 130 9 105 321 537 741 2.11e-13 71.2
Msa1266200 AT1G18080.1 30.811 185 117 5 11 189 147 326 2.16e-13 70.1
Msa1266200 AT1G18080.1 32.886 149 92 4 155 297 60 206 7.21e-12 65.9
Msa1266200 AT1G18080.1 25.191 262 157 7 34 272 79 324 6.73e-11 62.8
Msa1266200 AT1G53090.4 30.594 219 126 9 110 321 271 470 3.14e-13 70.9
Msa1266200 AT1G48630.1 31.293 147 96 3 155 297 60 205 3.50e-13 69.7
Msa1266200 AT1G48630.1 25.940 266 170 8 34 292 79 324 4.46e-12 66.2
Msa1266200 AT1G48630.1 29.189 185 120 5 11 189 146 325 5.84e-12 65.9
Msa1266200 AT1G48630.1 30.625 160 93 5 117 272 77 222 3.08e-11 63.9
Msa1266200 AT1G53090.3 30.594 219 126 9 110 321 492 691 5.10e-13 70.1
Msa1266200 AT1G53090.1 30.594 219 126 9 110 321 492 691 5.10e-13 70.1
Msa1266200 AT1G53090.2 30.594 219 126 9 110 321 492 691 5.10e-13 70.1
Msa1266200 AT3G16650.1 31.206 141 95 2 155 295 167 305 1.08e-12 68.9
Msa1266200 AT2G32700.2 24.818 274 176 11 1 270 539 786 1.40e-12 68.9
Msa1266200 AT2G32700.5 24.818 274 176 11 1 270 539 786 1.40e-12 68.9
Msa1266200 AT2G32700.1 24.818 274 176 11 1 270 539 786 1.40e-12 68.9
Msa1266200 AT2G32700.4 24.818 274 176 11 1 270 539 786 1.40e-12 68.9
Msa1266200 AT2G32700.3 24.818 274 176 11 1 270 539 786 1.40e-12 68.9
Msa1266200 AT2G32700.6 24.818 274 176 11 1 270 537 784 1.45e-12 68.9
Msa1266200 AT2G32700.7 24.818 274 176 11 1 270 558 805 1.49e-12 68.9
Msa1266200 AT3G18140.1 23.333 300 171 10 32 284 7 294 2.31e-12 67.0
Msa1266200 AT3G18130.1 24.620 329 195 12 6 292 7 324 2.48e-12 67.0
Msa1266200 AT3G18130.1 30.612 147 97 3 155 297 60 205 4.06e-12 66.6
Msa1266200 AT3G18130.1 27.568 185 123 5 11 189 146 325 3.95e-11 63.5
Msa1266200 AT3G15980.7 22.932 266 188 5 31 293 28 279 8.69e-12 66.6
Msa1266200 AT3G15980.7 24.153 236 162 4 63 295 18 239 6.35e-11 63.9
Msa1266200 AT3G15980.1 22.932 266 188 5 31 293 28 279 1.03e-11 66.2
Msa1266200 AT3G15980.1 24.153 236 162 4 63 295 18 239 7.13e-11 63.5
Msa1266200 AT3G15980.5 22.932 266 188 5 31 293 28 279 1.04e-11 66.2
Msa1266200 AT3G15980.5 24.153 236 162 4 63 295 18 239 8.29e-11 63.5
Msa1266200 AT3G15980.3 22.932 266 188 5 31 293 28 279 1.07e-11 66.2
Msa1266200 AT3G15980.3 24.153 236 162 4 63 295 18 239 8.19e-11 63.5
Msa1266200 AT3G15980.6 22.932 266 188 5 31 293 28 279 1.07e-11 66.2
Msa1266200 AT3G15980.6 24.153 236 162 4 63 295 18 239 8.19e-11 63.5
Msa1266200 AT3G15980.2 22.932 266 188 5 31 293 28 279 1.07e-11 66.2
Msa1266200 AT3G15980.2 24.153 236 162 4 63 295 18 239 8.19e-11 63.5
Msa1266200 AT3G15980.4 22.932 266 188 5 31 293 28 279 1.07e-11 66.2
Msa1266200 AT3G15980.4 24.153 236 162 4 63 295 18 239 8.19e-11 63.5
Msa1266200 AT1G73720.1 28.723 188 115 4 109 296 220 388 1.12e-11 65.9
Msa1266200 AT2G26060.2 25.410 244 146 10 95 318 13 240 1.25e-11 65.1
Msa1266200 AT2G26060.1 25.410 244 146 10 95 318 13 240 1.34e-11 65.1
Msa1266200 AT1G79990.2 24.413 213 146 4 81 291 78 277 2.15e-11 65.5
Msa1266200 AT1G79990.1 24.413 213 146 4 81 291 78 277 2.19e-11 65.5
Msa1266200 AT5G50230.1 26.855 283 176 11 11 285 222 481 3.01e-11 64.7
Msa1266200 AT5G16750.1 26.267 217 137 7 72 280 31 232 5.89e-11 63.9
Msa1266200 AT2G05720.1 27.976 168 97 2 152 295 57 224 8.21e-11 62.0
Msa1266200 AT1G52360.2 22.556 266 189 5 31 293 72 323 9.26e-11 63.5

Find 109 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAAAGATTGAACTATTTA+TGG 0.091497 8_2:+70961856 Msa1266200:three_prime_UTR
AGGTGGTCGGGTGGCGGTTG+CGG 0.205600 8_2:-70959128 None:intergenic
GTGGCAATAGCTGCCCCATC+TGG 0.222615 8_2:-70961484 None:intergenic
GGTGGTCGGGTGGCGGTTGC+GGG 0.251237 8_2:-70959127 None:intergenic
TTCTCTACTAATTGATTTGA+AGG 0.340653 8_2:+70961750 Msa1266200:three_prime_UTR
CGCTCACGACGATTCCGTTT+GGG 0.357973 8_2:+70959090 Msa1266200:CDS
CTTCTACACGAGGAATTGAT+AGG 0.378120 8_2:-70961147 None:intergenic
TCAACTCTACTTCACTTCGT+TGG 0.397174 8_2:-70958979 None:intergenic
ACGCTCACGACGATTCCGTT+TGG 0.403861 8_2:+70959089 Msa1266200:CDS
GGTGAGAAGGAGAGGTGGTC+GGG 0.404168 8_2:-70959140 None:intergenic
GCCTTAGTTGGGACCATGTC+AGG 0.407942 8_2:+70961427 Msa1266200:CDS
CAACTCTACTTCACTTCGTT+GGG 0.408639 8_2:-70958978 None:intergenic
CTATCAATTCCTCGTGTAGA+AGG 0.408966 8_2:+70961148 Msa1266200:CDS
GTTGTCTATCGATTTGATTC+CGG 0.412974 8_2:-70959068 None:intergenic
GGTGCCATTCTAGCAGTTGC+TGG 0.416864 8_2:+70961067 Msa1266200:CDS
AACAGTAGCCTTGGATCATA+AGG 0.425528 8_2:-70961355 None:intergenic
ATGTTTGGCTGGACTTTGAC+AGG 0.426014 8_2:+70961775 Msa1266200:three_prime_UTR
AAGCTCCTCCTTCTGAAGTC+TGG 0.428122 8_2:+70959353 Msa1266200:CDS
AAGAAATATGTGATGTGAAC+GGG 0.433906 8_2:+70958932 Msa1266200:five_prime_UTR
ATTGATTTGAAGGAAATGTT+TGG 0.440406 8_2:+70961760 Msa1266200:three_prime_UTR
CATGTCAGGACATGCTAGTT+GGG 0.445698 8_2:+70961441 Msa1266200:CDS
CAGGCATGAAGTGGCCTTCA+AGG 0.448183 8_2:-70961312 None:intergenic
GTGATAGAACTGTTAGGCTA+TGG 0.457317 8_2:+70961515 Msa1266200:CDS
AAGGAGAGGTGGTCGGGTGG+CGG 0.461374 8_2:-70959134 None:intergenic
GCTGAGGGAAAAGCCTTAGT+TGG 0.466242 8_2:+70961415 Msa1266200:CDS
TGGCAATAGCTGCCCCATCT+GGG 0.466304 8_2:-70961483 None:intergenic
TCCACCAGCAACTGCTAGAA+TGG 0.469517 8_2:-70961071 None:intergenic
TCCTGACATGGTCCCAACTA+AGG 0.472225 8_2:-70961428 None:intergenic
TCGACGAAACGGTACGCCTA+TGG 0.472782 8_2:+70959170 Msa1266200:CDS
TAGTGCATCAGTCAAACTTT+GGG 0.474897 8_2:+70961096 Msa1266200:CDS
ACGACTTGCTTGTGGCTCAA+TGG 0.474942 8_2:+70961240 Msa1266200:CDS
TCCGTTGCTGCTCATCCTCT+CGG 0.475436 8_2:+70959250 Msa1266200:CDS
GATACAGAGATATCTAAATC+AGG 0.476144 8_2:-70961689 None:intergenic
TACCGTTTCGTCGAGGGAGC+CGG 0.481975 8_2:-70959161 None:intergenic
TGCCCATGTGACTGCCCAAA+CGG 0.489414 8_2:-70959104 None:intergenic
CTTGCTTGTGGCTCAATGGA+TGG 0.490041 8_2:+70961244 Msa1266200:CDS
ATTTGAAGGAAATGTTTGGC+TGG 0.491783 8_2:+70961764 Msa1266200:three_prime_UTR
ACCAACACCGGCCACTGTCT+CGG 0.494590 8_2:+70959220 Msa1266200:CDS
TGTGTGGATGTGAGCCCAGA+TGG 0.495754 8_2:+70961469 Msa1266200:CDS
ATTCTAGCAGTTGCTGGTGG+AGG 0.496649 8_2:+70961073 Msa1266200:CDS
GAAGCTGAAAACAGTAGCCT+TGG 0.499277 8_2:-70961364 None:intergenic
ATTCCGTTTGGGCAGTCACA+TGG 0.502235 8_2:+70959101 Msa1266200:CDS
CACCGGCTCCCTCGACGAAA+CGG 0.502345 8_2:+70959159 Msa1266200:CDS
CCATGTCAGGACATGCTAGT+TGG 0.502674 8_2:+70961440 Msa1266200:CDS
CGCATTTGCCAGACTTCAGA+AGG 0.502981 8_2:-70959361 None:intergenic
TTTATTCTCCTTATGATCCA+AGG 0.502981 8_2:+70961347 Msa1266200:CDS
TCTGTTGCATGGAGCCCTGA+TGG 0.505116 8_2:+70961214 Msa1266200:CDS
GTTCAAGTGATAGAACTGTT+AGG 0.509508 8_2:+70961509 Msa1266200:CDS
GTAGTGCATCAGTCAAACTT+TGG 0.510035 8_2:+70961095 Msa1266200:CDS
ATATGTGATGTGAACGGGCT+TGG 0.516641 8_2:+70958937 Msa1266200:five_prime_UTR
CTGAGGGAAAAGCCTTAGTT+GGG 0.525517 8_2:+70961416 Msa1266200:CDS
CGTCGAGGGAGCCGGTGAGA+AGG 0.528073 8_2:-70959153 None:intergenic
GCCGAGACAGTGGCCGGTGT+TGG 0.530328 8_2:-70959221 None:intergenic
TGATAGAACTGTTAGGCTAT+GGG 0.537678 8_2:+70961516 Msa1266200:CDS
ACTTTGGGACACTTCTACGT+GGG 0.539380 8_2:+70961111 Msa1266200:CDS
CTTCGCACATCATTTCCTCC+AGG 0.542384 8_2:-70961613 None:intergenic
AGGGAGCCGGTGAGAAGGAG+AGG 0.544965 8_2:-70959148 None:intergenic
GATGGGGCAGCTATTGCCAC+AGG 0.547141 8_2:+70961487 Msa1266200:CDS
CCCAAACCCAGCGACAAAAG+TGG 0.550059 8_2:+70961172 Msa1266200:CDS
GCCATTCTAGCAGTTGCTGG+TGG 0.550553 8_2:+70961070 Msa1266200:CDS
GCAACCACACGGACCAAGTG+TGG 0.554761 8_2:+70961572 Msa1266200:CDS
CACGGACCAAGTGTGGGGAG+TGG 0.554970 8_2:+70961579 Msa1266200:CDS
TGTTGGTGCGTTCGAGAACA+AGG 0.555662 8_2:-70959204 None:intergenic
AGCGACGCCGAGACAGTGGC+CGG 0.557402 8_2:-70959227 None:intergenic
CGGTGAGAAGGAGAGGTGGT+CGG 0.558774 8_2:-70959141 None:intergenic
TCCGAGAGGATGAGCAGCAA+CGG 0.561208 8_2:-70959251 None:intergenic
AATTTGTCCTGTCTGTTGCA+TGG 0.564640 8_2:+70961203 Msa1266200:CDS
CAGGGCTCCATGCAACAGAC+AGG 0.567813 8_2:-70961210 None:intergenic
CAAGGTCATCGGATTTCCAT+AGG 0.573281 8_2:-70959186 None:intergenic
CGCACATCATTTCCTCCAGG+TGG 0.573491 8_2:-70961610 None:intergenic
CGGAAGCGACGCCGAGACAG+TGG 0.574570 8_2:-70959231 None:intergenic
TAAATGCCACTCCCCACACT+TGG 0.575870 8_2:-70961585 None:intergenic
AATCTTTAAATGCAATGACT+AGG 0.577073 8_2:-70961843 None:intergenic
GAGAAGGAGAGGTGGTCGGG+TGG 0.578252 8_2:-70959137 None:intergenic
GGAGTGGCATTTAGACCACC+TGG 0.580192 8_2:+70961595 Msa1266200:CDS
GCAAATGCGTTTCGATCCCA+AGG 0.583049 8_2:+70959375 Msa1266200:CDS
GAGCCGGTGAGAAGGAGAGG+TGG 0.586712 8_2:-70959145 None:intergenic
CAAGAAATATGTGATGTGAA+CGG 0.592636 8_2:+70958931 None:intergenic
GATGGAAAACGACTTGCTTG+TGG 0.596181 8_2:+70961232 Msa1266200:CDS
TGTTCACATGTATGATGCTG+AGG 0.597783 8_2:+70961399 Msa1266200:CDS
CAACCACACGGACCAAGTGT+GGG 0.600837 8_2:+70961573 Msa1266200:CDS
AGCTTCAAGAGTAGCGACAG+TGG 0.603641 8_2:-70959335 None:intergenic
GCGTTCGAGAACAAGGTCAT+CGG 0.604223 8_2:-70959197 None:intergenic
TTCTACACGAGGAATTGATA+GGG 0.604233 8_2:-70961146 None:intergenic
GTGTGGATGTGAGCCCAGAT+GGG 0.605196 8_2:+70961470 Msa1266200:CDS
GTTGTTGCAATGAAACTCGC+CGG 0.606480 8_2:+70959049 Msa1266200:exon
TTCCGTTTGGGCAGTCACAT+GGG 0.606559 8_2:+70959102 Msa1266200:CDS
GTTCTCGAACGCACCAACAC+CGG 0.609099 8_2:+70959208 Msa1266200:CDS
GGCTATGGGATCTTAACATG+AGG 0.612345 8_2:+70961530 Msa1266200:CDS
GCTATCAAGAGAAGAAGAAG+CGG 0.616284 8_2:-70959281 None:intergenic
GAGAACGCACAGGCATGAAG+TGG 0.618047 8_2:-70961321 None:intergenic
CGACCACCTCTCCTTCTCAC+CGG 0.619054 8_2:+70959142 Msa1266200:CDS
CCAACTAGCATGTCCTGACA+TGG 0.632027 8_2:-70961440 None:intergenic
AGTTCTATCACTTGAACCTG+TGG 0.635592 8_2:-70961503 None:intergenic
ATAGGCGTACCGTTTCGTCG+AGG 0.643084 8_2:-70959168 None:intergenic
AACCACACGGACCAAGTGTG+GGG 0.646543 8_2:+70961574 Msa1266200:CDS
CTTGGTAAACTAATAGAGAG+AGG 0.651831 8_2:+70958955 Msa1266200:five_prime_UTR
GAAATGATGTGCGAAGCGGT+CGG 0.656755 8_2:+70961620 Msa1266200:CDS
GTTCACATGTATGATGCTGA+GGG 0.658597 8_2:+70961400 Msa1266200:CDS
ATTTGCCAGACTTCAGAAGG+AGG 0.661343 8_2:-70959358 None:intergenic
GCTATGGGATCTTAACATGA+GGG 0.661632 8_2:+70961531 Msa1266200:CDS
GAATAAACAAGAGAACGCAC+AGG 0.670302 8_2:-70961331 None:intergenic
GGTTTGGGTCCTTCTACACG+AGG 0.670981 8_2:-70961157 None:intergenic
AACTTTGGGACACTTCTACG+TGG 0.681297 8_2:+70961110 Msa1266200:CDS
TGTGGATGTGAGCCCAGATG+GGG 0.693493 8_2:+70961471 Msa1266200:CDS
GGAGGAAATGATGTGCGAAG+CGG 0.697673 8_2:+70961616 Msa1266200:CDS
GAAGCGGCAATTGATCCGAG+AGG 0.698535 8_2:-70959265 None:intergenic
GCAGACGATGAGCAACCACA+CGG 0.725867 8_2:+70961561 Msa1266200:CDS
GTGGCATTTAGACCACCTGG+AGG 0.789300 8_2:+70961598 Msa1266200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAAAAATAAAAATTGA+TGG + chr8_2:70959558-70959577 Msa1266200:intron 10.0%
!! AAATGTTTACAAAAAAAAAT+AGG + chr8_2:70959914-70959933 Msa1266200:intron 10.0%
!!! TTATAGAATTCTCTAAAATT+TGG - chr8_2:70959464-70959483 None:intergenic 15.0%
!!! TAGTTTTGATAATGTAAATT+AGG - chr8_2:70959533-70959552 None:intergenic 15.0%
!!! TATTTTTCGTTTCTAAATTA+AGG + chr8_2:70959883-70959902 Msa1266200:intron 15.0%
!! ATATATCAAAATATTGACAT+AGG + chr8_2:70960004-70960023 Msa1266200:intron 15.0%
!!! GTATTGAAATTTTCTTTTTT+TGG + chr8_2:70960560-70960579 Msa1266200:intron 15.0%
!!! CAAGATTTCTATTAGTTTTT+TGG + chr8_2:70959499-70959518 Msa1266200:intron 20.0%
!!! AGTAAAAATGTAGTACATTT+TGG + chr8_2:70959698-70959717 Msa1266200:intron 20.0%
!!! ATATAAATGCATCTGTTTTA+TGG + chr8_2:70960374-70960393 Msa1266200:intron 20.0%
!!! TATAAATGCATCTGTTTTAT+GGG + chr8_2:70960375-70960394 Msa1266200:intron 20.0%
!! ATACATTCTTAACATAAAGT+GGG - chr8_2:70960742-70960761 None:intergenic 20.0%
!! GTAAATTTGAAGCAAATTGA+TGG - chr8_2:70959411-70959430 None:intergenic 25.0%
! TGACATGTTACAAATTAGAA+TGG + chr8_2:70959614-70959633 Msa1266200:intron 25.0%
!!! ATGTGTTTTCTTTTTAGTGT+TGG + chr8_2:70959646-70959665 Msa1266200:intron 25.0%
!!! TGTGTTTTCTTTTTAGTGTT+GGG + chr8_2:70959647-70959666 Msa1266200:intron 25.0%
! GTTCTATTAGAAAAGTAGAA+TGG + chr8_2:70959720-70959739 Msa1266200:intron 25.0%
! TTAAACTAATTACCCAAACA+GGG - chr8_2:70960030-70960049 None:intergenic 25.0%
! ATTAAACTAATTACCCAAAC+AGG - chr8_2:70960031-70960050 None:intergenic 25.0%
! ATTCATAACACAAGCATTTA+GGG - chr8_2:70960331-70960350 None:intergenic 25.0%
!! ATAAATGCATCTGTTTTATG+GGG + chr8_2:70960376-70960395 Msa1266200:intron 25.0%
!!! CACTTTTTAACATCATTGTT+GGG + chr8_2:70960428-70960447 Msa1266200:intron 25.0%
!! GCAAAAATGCTTTTACTTTA+CGG - chr8_2:70960496-70960515 None:intergenic 25.0%
! TGCAAACATAGTATTTAAAC+AGG - chr8_2:70960620-70960639 None:intergenic 25.0%
! AATCAAGATAACTGACATAT+AGG - chr8_2:70960706-70960725 None:intergenic 25.0%
! GATACATTCTTAACATAAAG+TGG - chr8_2:70960743-70960762 None:intergenic 25.0%
! CATATATGGTTCACATAAAA+GGG - chr8_2:70960903-70960922 None:intergenic 25.0%
! AATATTATGCACATAGATAG+TGG - chr8_2:70960928-70960947 None:intergenic 25.0%
! TCTATGTGCATAATATTCAT+AGG + chr8_2:70960931-70960950 Msa1266200:intron 25.0%
!!! TTCTCTACTAATTGATTTGA+AGG + chr8_2:70961750-70961769 Msa1266200:three_prime_UTR 25.0%
!!! ATTGATTTGAAGGAAATGTT+TGG + chr8_2:70961760-70961779 Msa1266200:three_prime_UTR 25.0%
! AATCTTTAAATGCAATGACT+AGG - chr8_2:70961846-70961865 None:intergenic 25.0%
AAGAAATATGTGATGTGAAC+GGG + chr8_2:70958932-70958951 Msa1266200:five_prime_UTR 30.0%
TGATGGAATTGAATTACCTT+GGG - chr8_2:70959394-70959413 None:intergenic 30.0%
TTGATGGAATTGAATTACCT+TGG - chr8_2:70959395-70959414 None:intergenic 30.0%
! ATATTTTCCCTGGATAACTT+AGG - chr8_2:70959790-70959809 None:intergenic 30.0%
GCTAAACTTAACTCATCTAA+GGG + chr8_2:70959816-70959835 Msa1266200:intron 30.0%
!!! TATGTTTTGATGGAGAGTTT+TGG + chr8_2:70959839-70959858 Msa1266200:intron 30.0%
CTATTTACATAAGCACTTGT+GGG - chr8_2:70960245-70960264 None:intergenic 30.0%
TCTATTTACATAAGCACTTG+TGG - chr8_2:70960246-70960265 None:intergenic 30.0%
TATGTTTGGATTGAAGATTC+TGG - chr8_2:70960308-70960327 None:intergenic 30.0%
GATTCATAACACAAGCATTT+AGG - chr8_2:70960332-70960351 None:intergenic 30.0%
ATGCTTGTGTTATGAATCAA+CGG + chr8_2:70960334-70960353 Msa1266200:intron 30.0%
CCAACAATGATGTTAAAAAG+TGG - chr8_2:70960430-70960449 None:intergenic 30.0%
!!! CCACTTTTTAACATCATTGT+TGG + chr8_2:70960427-70960446 Msa1266200:intron 30.0%
! TTTTAACATCATTGTTGGGA+TGG + chr8_2:70960432-70960451 Msa1266200:intron 30.0%
TTTAACATCATTGTTGGGAT+GGG + chr8_2:70960433-70960452 Msa1266200:intron 30.0%
ACACAAAAACAAGTAGTAAC+CGG + chr8_2:70960463-70960482 Msa1266200:intron 30.0%
TGGGTATCACAAGAAAATAT+TGG - chr8_2:70960518-70960537 None:intergenic 30.0%
CAATCAACTTCATGAGATAT+AGG - chr8_2:70960667-70960686 None:intergenic 30.0%
! CTATATCTCATGAAGTTGAT+TGG + chr8_2:70960665-70960684 Msa1266200:intron 30.0%
AATAGTCCATTATTGCATAC+AGG + chr8_2:70960793-70960812 Msa1266200:intron 30.0%
ATAGTCCATTATTGCATACA+GGG + chr8_2:70960794-70960813 Msa1266200:intron 30.0%
ACATATTAACAGTGAATCGA+CGG - chr8_2:70960879-70960898 None:intergenic 30.0%
CCATATATGGTTCACATAAA+AGG - chr8_2:70960904-70960923 None:intergenic 30.0%
! CCTTTTATGTGAACCATATA+TGG + chr8_2:70960901-70960920 Msa1266200:intron 30.0%
CGTTATATACAAGTGTCAAA+AGG - chr8_2:70960972-70960991 None:intergenic 30.0%
!! GCAAAAACTATTATCGATTC+TGG - chr8_2:70961005-70961024 None:intergenic 30.0%
ATTAAAAGTTCTCAACCTTC+AGG + chr8_2:70961045-70961064 Msa1266200:intron 30.0%
TTAAAAGTTCTCAACCTTCA+GGG + chr8_2:70961046-70961065 Msa1266200:intron 30.0%
TTGCACATCAAAAACAGAAA+TGG - chr8_2:70961272-70961291 None:intergenic 30.0%
TTCAAGGTGATGCAAAAATT+TGG - chr8_2:70961299-70961318 None:intergenic 30.0%
! AAATTTTTGCATCACCTTGA+AGG + chr8_2:70961298-70961317 Msa1266200:CDS 30.0%
TTTATTCTCCTTATGATCCA+AGG + chr8_2:70961347-70961366 Msa1266200:CDS 30.0%
GATACAGAGATATCTAAATC+AGG - chr8_2:70961692-70961711 None:intergenic 30.0%
CTTGGTAAACTAATAGAGAG+AGG + chr8_2:70958955-70958974 Msa1266200:five_prime_UTR 35.0%
!! GTTGTCTATCGATTTGATTC+CGG - chr8_2:70959071-70959090 None:intergenic 35.0%
! TGTTTTGTCCTAAGTTATCC+AGG + chr8_2:70959779-70959798 Msa1266200:intron 35.0%
! GTTTTGTCCTAAGTTATCCA+GGG + chr8_2:70959780-70959799 Msa1266200:intron 35.0%
TATCCAGGGAAAATATCTGT+TGG + chr8_2:70959794-70959813 Msa1266200:intron 35.0%
GGCTAAACTTAACTCATCTA+AGG + chr8_2:70959815-70959834 Msa1266200:intron 35.0%
!! CATCTAAGGGTATGTTTTGA+TGG + chr8_2:70959829-70959848 Msa1266200:intron 35.0%
TTTGGATTGAAGATTCTGGA+GGG - chr8_2:70960304-70960323 None:intergenic 35.0%
! ACAAGCATTTAGGGTATGTT+TGG - chr8_2:70960322-70960341 None:intergenic 35.0%
TTTACTTTACGGAAGTTAGC+CGG - chr8_2:70960485-70960504 None:intergenic 35.0%
TGCTACAAATGTGAAATCGT+GGG - chr8_2:70960537-70960556 None:intergenic 35.0%
ATGCTACAAATGTGAAATCG+TGG - chr8_2:70960538-70960557 None:intergenic 35.0%
!! ATAATGGACTATTCTGAGAC+TGG - chr8_2:70960786-70960805 None:intergenic 35.0%
TAGTCCATTATTGCATACAG+GGG + chr8_2:70960795-70960814 Msa1266200:intron 35.0%
AGCTTTCAGTATAAGAACTG+AGG - chr8_2:70960832-70960851 None:intergenic 35.0%
!! CATAGATAGTGGACCATATA+TGG - chr8_2:70960917-70960936 None:intergenic 35.0%
! TAATAGTTTTTGCCACACAG+TGG + chr8_2:70961012-70961031 Msa1266200:intron 35.0%
ATGATTAAACAACCACTGTG+TGG - chr8_2:70961027-70961046 None:intergenic 35.0%
TAGTGCATCAGTCAAACTTT+GGG + chr8_2:70961096-70961115 Msa1266200:CDS 35.0%
TTCTACACGAGGAATTGATA+GGG - chr8_2:70961149-70961168 None:intergenic 35.0%
GTTCAAGTGATAGAACTGTT+AGG + chr8_2:70961509-70961528 Msa1266200:CDS 35.0%
TGATAGAACTGTTAGGCTAT+GGG + chr8_2:70961516-70961535 Msa1266200:CDS 35.0%
ATTTGAAGGAAATGTTTGGC+TGG + chr8_2:70961764-70961783 Msa1266200:three_prime_UTR 35.0%
CAACTCTACTTCACTTCGTT+GGG - chr8_2:70958981-70959000 None:intergenic 40.0%
TCAACTCTACTTCACTTCGT+TGG - chr8_2:70958982-70959001 None:intergenic 40.0%
GCTATCAAGAGAAGAAGAAG+CGG - chr8_2:70959284-70959303 None:intergenic 40.0%
! TAGCCAACAGATATTTTCCC+TGG - chr8_2:70959800-70959819 None:intergenic 40.0%
!! GTTTTGATGGAGAGTTTTGG+AGG + chr8_2:70959842-70959861 Msa1266200:intron 40.0%
ATATTGACATAGGCCCTGTT+TGG + chr8_2:70960014-70960033 Msa1266200:intron 40.0%
TATTGACATAGGCCCTGTTT+GGG + chr8_2:70960015-70960034 Msa1266200:intron 40.0%
GTTTGGATTGAAGATTCTGG+AGG - chr8_2:70960305-70960324 None:intergenic 40.0%
! TGCATCTGTTTTATGGGGAA+AGG + chr8_2:70960381-70960400 Msa1266200:intron 40.0%
! GGATTTTGCTTCCAATTCTG+CGG + chr8_2:70960402-70960421 Msa1266200:intron 40.0%
!!! TTCTTTTTTTGGTCCTGCAC+TGG + chr8_2:70960571-70960590 Msa1266200:intron 40.0%
GGTTACATGCGTAAGTAAGT+TGG - chr8_2:70960765-70960784 None:intergenic 40.0%
CGACGGAACAAGGAATTAAA+TGG - chr8_2:70960862-70960881 None:intergenic 40.0%
GTAGTGCATCAGTCAAACTT+TGG + chr8_2:70961095-70961114 Msa1266200:CDS 40.0%
CTTCTACACGAGGAATTGAT+AGG - chr8_2:70961150-70961169 None:intergenic 40.0%
CTATCAATTCCTCGTGTAGA+AGG + chr8_2:70961148-70961167 Msa1266200:CDS 40.0%
AATTTGTCCTGTCTGTTGCA+TGG + chr8_2:70961203-70961222 Msa1266200:CDS 40.0%
!! AAGCAAGTCGTTTTCCATCA+GGG - chr8_2:70961231-70961250 None:intergenic 40.0%
GAATAAACAAGAGAACGCAC+AGG - chr8_2:70961334-70961353 None:intergenic 40.0%
! AACAGTAGCCTTGGATCATA+AGG - chr8_2:70961358-70961377 None:intergenic 40.0%
! CTGTTTTCAGCTTCAGATGA+TGG + chr8_2:70961373-70961392 Msa1266200:CDS 40.0%
TGTTCACATGTATGATGCTG+AGG + chr8_2:70961399-70961418 Msa1266200:CDS 40.0%
GTTCACATGTATGATGCTGA+GGG + chr8_2:70961400-70961419 Msa1266200:CDS 40.0%
!! TGCTAGTTGGGTTTTATGTG+TGG + chr8_2:70961453-70961472 Msa1266200:CDS 40.0%
AGTTCTATCACTTGAACCTG+TGG - chr8_2:70961506-70961525 None:intergenic 40.0%
GTGATAGAACTGTTAGGCTA+TGG + chr8_2:70961515-70961534 Msa1266200:CDS 40.0%
GCTATGGGATCTTAACATGA+GGG + chr8_2:70961531-70961550 Msa1266200:CDS 40.0%
! ATATGTGATGTGAACGGGCT+TGG + chr8_2:70958937-70958956 Msa1266200:five_prime_UTR 45.0%
GTTGTTGCAATGAAACTCGC+CGG + chr8_2:70959049-70959068 Msa1266200:exon 45.0%
CAAGGTCATCGGATTTCCAT+AGG - chr8_2:70959189-70959208 None:intergenic 45.0%
ATTTGCCAGACTTCAGAAGG+AGG - chr8_2:70959361-70959380 None:intergenic 45.0%
! TGGAGGGCTAAGAACTTGTT+TGG - chr8_2:70960288-70960307 None:intergenic 45.0%
AAAAAGTGGCACCGCAGAAT+TGG - chr8_2:70960416-70960435 None:intergenic 45.0%
AATTGCAAACACACCAGTGC+AGG - chr8_2:70960587-70960606 None:intergenic 45.0%
! TGCACTGGTGTGTTTGCAAT+TGG + chr8_2:70960586-70960605 Msa1266200:intron 45.0%
CAAGCCCCTGTATGCAATAA+TGG - chr8_2:70960802-70960821 None:intergenic 45.0%
AACAGTGAATCGACGGAACA+AGG - chr8_2:70960872-70960891 None:intergenic 45.0%
!! AACTTTGGGACACTTCTACG+TGG + chr8_2:70961110-70961129 Msa1266200:CDS 45.0%
!! ACTTTGGGACACTTCTACGT+GGG + chr8_2:70961111-70961130 Msa1266200:CDS 45.0%
!! CAAGCAAGTCGTTTTCCATC+AGG - chr8_2:70961232-70961251 None:intergenic 45.0%
! GATGGAAAACGACTTGCTTG+TGG + chr8_2:70961232-70961251 Msa1266200:CDS 45.0%
GAAGCTGAAAACAGTAGCCT+TGG - chr8_2:70961367-70961386 None:intergenic 45.0%
CTGAGGGAAAAGCCTTAGTT+GGG + chr8_2:70961416-70961435 Msa1266200:CDS 45.0%
CATGTCAGGACATGCTAGTT+GGG + chr8_2:70961441-70961460 Msa1266200:CDS 45.0%
GGCTATGGGATCTTAACATG+AGG + chr8_2:70961530-70961549 Msa1266200:CDS 45.0%
ATGTTTGGCTGGACTTTGAC+AGG + chr8_2:70961775-70961794 Msa1266200:three_prime_UTR 45.0%
ATTCCGTTTGGGCAGTCACA+TGG + chr8_2:70959101-70959120 Msa1266200:CDS 50.0%
TTCCGTTTGGGCAGTCACAT+GGG + chr8_2:70959102-70959121 Msa1266200:CDS 50.0%
GCGTTCGAGAACAAGGTCAT+CGG - chr8_2:70959200-70959219 None:intergenic 50.0%
TGTTGGTGCGTTCGAGAACA+AGG - chr8_2:70959207-70959226 None:intergenic 50.0%
AGCTTCAAGAGTAGCGACAG+TGG - chr8_2:70959338-70959357 None:intergenic 50.0%
!! AAGCTCCTCCTTCTGAAGTC+TGG + chr8_2:70959353-70959372 Msa1266200:CDS 50.0%
CGCATTTGCCAGACTTCAGA+AGG - chr8_2:70959364-70959383 None:intergenic 50.0%
GCAAATGCGTTTCGATCCCA+AGG + chr8_2:70959375-70959394 Msa1266200:CDS 50.0%
!!! GATGGAGAGTTTTGGAGGAG+AGG + chr8_2:70959847-70959866 Msa1266200:intron 50.0%
!!! AGTTTTGGAGGAGAGGAGAG+TGG + chr8_2:70959854-70959873 Msa1266200:intron 50.0%
TCCACCAGCAACTGCTAGAA+TGG - chr8_2:70961074-70961093 None:intergenic 50.0%
ATTCTAGCAGTTGCTGGTGG+AGG + chr8_2:70961073-70961092 Msa1266200:CDS 50.0%
!! CCACTTTTGTCGCTGGGTTT+GGG - chr8_2:70961175-70961194 None:intergenic 50.0%
!!! TTGCTGCCACTTTTGTCGCT+GGG - chr8_2:70961181-70961200 None:intergenic 50.0%
! ACGACTTGCTTGTGGCTCAA+TGG + chr8_2:70961240-70961259 Msa1266200:CDS 50.0%
CTTGCTTGTGGCTCAATGGA+TGG + chr8_2:70961244-70961263 Msa1266200:CDS 50.0%
GCTGAGGGAAAAGCCTTAGT+TGG + chr8_2:70961415-70961434 Msa1266200:CDS 50.0%
TCCTGACATGGTCCCAACTA+AGG - chr8_2:70961431-70961450 None:intergenic 50.0%
CCAACTAGCATGTCCTGACA+TGG - chr8_2:70961443-70961462 None:intergenic 50.0%
CCATGTCAGGACATGCTAGT+TGG + chr8_2:70961440-70961459 Msa1266200:CDS 50.0%
TAAATGCCACTCCCCACACT+TGG - chr8_2:70961588-70961607 None:intergenic 50.0%
CTTCGCACATCATTTCCTCC+AGG - chr8_2:70961616-70961635 None:intergenic 50.0%
GGAGGAAATGATGTGCGAAG+CGG + chr8_2:70961616-70961635 Msa1266200:CDS 50.0%
GAAATGATGTGCGAAGCGGT+CGG + chr8_2:70961620-70961639 Msa1266200:CDS 50.0%
ACGCTCACGACGATTCCGTT+TGG + chr8_2:70959089-70959108 Msa1266200:CDS 55.0%
CGCTCACGACGATTCCGTTT+GGG + chr8_2:70959090-70959109 Msa1266200:CDS 55.0%
TGCCCATGTGACTGCCCAAA+CGG - chr8_2:70959107-70959126 None:intergenic 55.0%
TAGGCGTACCGTTTCGTCGA+GGG - chr8_2:70959170-70959189 None:intergenic 55.0%
ATAGGCGTACCGTTTCGTCG+AGG - chr8_2:70959171-70959190 None:intergenic 55.0%
TCGACGAAACGGTACGCCTA+TGG + chr8_2:70959170-70959189 Msa1266200:CDS 55.0%
GTTCTCGAACGCACCAACAC+CGG + chr8_2:70959208-70959227 Msa1266200:CDS 55.0%
TCCGTTGCTGCTCATCCTCT+CGG + chr8_2:70959250-70959269 Msa1266200:CDS 55.0%
TCCGAGAGGATGAGCAGCAA+CGG - chr8_2:70959254-70959273 None:intergenic 55.0%
GAAGCGGCAATTGATCCGAG+AGG - chr8_2:70959268-70959287 None:intergenic 55.0%
TTTGGAGGAGAGGAGAGTGG+AGG + chr8_2:70959857-70959876 Msa1266200:intron 55.0%
CTGCTAGAATGGCACCCTGA+AGG - chr8_2:70961063-70961082 None:intergenic 55.0%
!! GGTGCCATTCTAGCAGTTGC+TGG + chr8_2:70961067-70961086 Msa1266200:CDS 55.0%
GCCATTCTAGCAGTTGCTGG+TGG + chr8_2:70961070-70961089 Msa1266200:CDS 55.0%
GGTTTGGGTCCTTCTACACG+AGG - chr8_2:70961160-70961179 None:intergenic 55.0%
CCCAAACCCAGCGACAAAAG+TGG + chr8_2:70961172-70961191 Msa1266200:CDS 55.0%
!!! GCCACTTTTGTCGCTGGGTT+TGG - chr8_2:70961176-70961195 None:intergenic 55.0%
!!! CTTGCTGCCACTTTTGTCGC+TGG - chr8_2:70961182-70961201 None:intergenic 55.0%
TCTGTTGCATGGAGCCCTGA+TGG + chr8_2:70961214-70961233 Msa1266200:CDS 55.0%
CAGGCATGAAGTGGCCTTCA+AGG - chr8_2:70961315-70961334 None:intergenic 55.0%
GAGAACGCACAGGCATGAAG+TGG - chr8_2:70961324-70961343 None:intergenic 55.0%
GCCTTAGTTGGGACCATGTC+AGG + chr8_2:70961427-70961446 Msa1266200:CDS 55.0%
TGTGTGGATGTGAGCCCAGA+TGG + chr8_2:70961469-70961488 Msa1266200:CDS 55.0%
GTGTGGATGTGAGCCCAGAT+GGG + chr8_2:70961470-70961489 Msa1266200:CDS 55.0%
TGTGGATGTGAGCCCAGATG+GGG + chr8_2:70961471-70961490 Msa1266200:CDS 55.0%
TGGCAATAGCTGCCCCATCT+GGG - chr8_2:70961486-70961505 None:intergenic 55.0%
GCAGACGATGAGCAACCACA+CGG + chr8_2:70961561-70961580 Msa1266200:CDS 55.0%
CAACCACACGGACCAAGTGT+GGG + chr8_2:70961573-70961592 Msa1266200:CDS 55.0%
AACCACACGGACCAAGTGTG+GGG + chr8_2:70961574-70961593 Msa1266200:CDS 55.0%
GGAGTGGCATTTAGACCACC+TGG + chr8_2:70961595-70961614 Msa1266200:CDS 55.0%
GTGGCATTTAGACCACCTGG+AGG + chr8_2:70961598-70961617 Msa1266200:CDS 55.0%
CGCACATCATTTCCTCCAGG+TGG - chr8_2:70961613-70961632 None:intergenic 55.0%
GGTGAGAAGGAGAGGTGGTC+GGG - chr8_2:70959143-70959162 None:intergenic 60.0%
CGGTGAGAAGGAGAGGTGGT+CGG - chr8_2:70959144-70959163 None:intergenic 60.0%
CGACCACCTCTCCTTCTCAC+CGG + chr8_2:70959142-70959161 Msa1266200:CDS 60.0%
TACCGTTTCGTCGAGGGAGC+CGG - chr8_2:70959164-70959183 None:intergenic 60.0%
ACCAACACCGGCCACTGTCT+CGG + chr8_2:70959220-70959239 Msa1266200:CDS 60.0%
CAGGGCTCCATGCAACAGAC+AGG - chr8_2:70961213-70961232 None:intergenic 60.0%
GTGGCAATAGCTGCCCCATC+TGG - chr8_2:70961487-70961506 None:intergenic 60.0%
GATGGGGCAGCTATTGCCAC+AGG + chr8_2:70961487-70961506 Msa1266200:CDS 60.0%
GCAACCACACGGACCAAGTG+TGG + chr8_2:70961572-70961591 Msa1266200:CDS 60.0%
AAGGAGAGGTGGTCGGGTGG+CGG - chr8_2:70959137-70959156 None:intergenic 65.0%
GAGAAGGAGAGGTGGTCGGG+TGG - chr8_2:70959140-70959159 None:intergenic 65.0%
GAGCCGGTGAGAAGGAGAGG+TGG - chr8_2:70959148-70959167 None:intergenic 65.0%
AGGGAGCCGGTGAGAAGGAG+AGG - chr8_2:70959151-70959170 None:intergenic 65.0%
!! CACCGGCTCCCTCGACGAAA+CGG + chr8_2:70959159-70959178 Msa1266200:CDS 65.0%
CTCCCCACACTTGGTCCGTG+TGG - chr8_2:70961579-70961598 None:intergenic 65.0%
CACGGACCAAGTGTGGGGAG+TGG + chr8_2:70961579-70961598 Msa1266200:CDS 65.0%
! AGGTGGTCGGGTGGCGGTTG+CGG - chr8_2:70959131-70959150 None:intergenic 70.0%
CGTCGAGGGAGCCGGTGAGA+AGG - chr8_2:70959156-70959175 None:intergenic 70.0%
GCCGAGACAGTGGCCGGTGT+TGG - chr8_2:70959224-70959243 None:intergenic 70.0%
AGCGACGCCGAGACAGTGGC+CGG - chr8_2:70959230-70959249 None:intergenic 70.0%
CGGAAGCGACGCCGAGACAG+TGG - chr8_2:70959234-70959253 None:intergenic 70.0%
! GGTGGTCGGGTGGCGGTTGC+GGG - chr8_2:70959130-70959149 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 70958932 70961878 70958932 ID=Msa1266200;Name=Msa1266200
chr8_2 mRNA 70958932 70961878 70958932 ID=Msa1266200-mRNA-1;Parent=Msa1266200;Name=Msa1266200-mRNA-1;_AED=0.00;_eAED=0.00;_QI=126|1|1|1|1|1|2|185|321
chr8_2 exon 70958932 70959396 70958932 ID=Msa1266200-mRNA-1:exon:23614;Parent=Msa1266200-mRNA-1
chr8_2 exon 70961067 70961878 70961067 ID=Msa1266200-mRNA-1:exon:23615;Parent=Msa1266200-mRNA-1
chr8_2 five_prime_UTR 70958932 70959057 70958932 ID=Msa1266200-mRNA-1:five_prime_utr;Parent=Msa1266200-mRNA-1
chr8_2 CDS 70959058 70959396 70959058 ID=Msa1266200-mRNA-1:cds;Parent=Msa1266200-mRNA-1
chr8_2 CDS 70961067 70961693 70961067 ID=Msa1266200-mRNA-1:cds;Parent=Msa1266200-mRNA-1
chr8_2 three_prime_UTR 70961694 70961878 70961694 ID=Msa1266200-mRNA-1:three_prime_utr;Parent=Msa1266200-mRNA-1
Gene Sequence

>Msa1266200

ATGAAACTCGCCGGAATCAAATCGATAGACAACGCTCACGACGATTCCGTTTGGGCAGTCACATGGGCACCCGCAACCGCCACCCGACCACCTCTCCTTCTCACCGGCTCCCTCGACGAAACGGTACGCCTATGGAAATCCGATGACCTTGTTCTCGAACGCACCAACACCGGCCACTGTCTCGGCGTCGCTTCCGTTGCTGCTCATCCTCTCGGATCAATTGCCGCTTCTTCTTCTCTTGATAGCTTTGTTCGTGTCTTTGATGTTGATTCTAATGCCACTGTCGCTACTCTTGAAGCTCCTCCTTCTGAAGTCTGGCAAATGCGTTTCGATCCCAAGGGTGCCATTCTAGCAGTTGCTGGTGGAGGTAGTGCATCAGTCAAACTTTGGGACACTTCTACGTGGGAACTTGTTGTCACCCTATCAATTCCTCGTGTAGAAGGACCCAAACCCAGCGACAAAAGTGGCAGCAAGAAATTTGTCCTGTCTGTTGCATGGAGCCCTGATGGAAAACGACTTGCTTGTGGCTCAATGGATGGAACCATTTCTGTTTTTGATGTGCAACGAGCCAAATTTTTGCATCACCTTGAAGGCCACTTCATGCCTGTGCGTTCTCTTGTTTATTCTCCTTATGATCCAAGGCTACTGTTTTCAGCTTCAGATGATGGTAATGTTCACATGTATGATGCTGAGGGAAAAGCCTTAGTTGGGACCATGTCAGGACATGCTAGTTGGGTTTTATGTGTGGATGTGAGCCCAGATGGGGCAGCTATTGCCACAGGTTCAAGTGATAGAACTGTTAGGCTATGGGATCTTAACATGAGGGCGTCTGTGCAGACGATGAGCAACCACACGGACCAAGTGTGGGGAGTGGCATTTAGACCACCTGGAGGAAATGATGTGCGAAGCGGTCGGCTTGCTAGTGTATCAGATGATAAGAGCATATCGCTGTATGATTATTCCTGA
Protein sequence

>Msa1266200

MKLAGIKSIDNAHDDSVWAVTWAPATATRPPLLLTGSLDETVRLWKSDDLVLERTNTGHCLGVASVAAHPLGSIAASSSLDSFVRVFDVDSNATVATLEAPPSEVWQMRFDPKGAILAVAGGGSASVKLWDTSTWELVVTLSIPRVEGPKPSDKSGSKKFVLSVAWSPDGKRLACGSMDGTISVFDVQRAKFLHHLEGHFMPVRSLVYSPYDPRLLFSASDDGNVHMYDAEGKALVGTMSGHASWVLCVDVSPDGAAIATGSSDRTVRLWDLNMRASVQTMSNHTDQVWGVAFRPPGGNDVRSGRLASVSDDKSISLYDYS*