AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1277210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1277210 MtrunA17_Chr8g0345361 95.473 243 8 2 1 243 1 240 3.31e-171 474
Msa1277210 MtrunA17_Chr7g0276591 41.434 251 111 5 19 265 12 230 7.18e-46 157
Msa1277210 MtrunA17_Chr4g0067591 42.697 178 76 7 23 183 13 181 8.02e-33 122
Msa1277210 MtrunA17_Chr2g0295571 36.994 173 92 5 21 181 23 190 6.54e-31 117
Msa1277210 MtrunA17_Chr3g0105441 37.931 174 90 3 30 185 25 198 5.46e-24 98.6
Msa1277210 MtrunA17_Chr5g0426781 28.846 208 142 4 31 234 63 268 8.77e-24 99.8
Msa1277210 MtrunA17_Chr4g0059281 34.359 195 95 7 31 206 48 228 2.97e-22 94.7
Msa1277210 MtrunA17_Chr5g0413841 35.507 138 80 4 33 162 49 185 3.82e-22 95.5
Msa1277210 MtrunA17_Chr5g0407431 30.726 179 99 3 32 207 33 189 9.82e-22 91.7
Msa1277210 MtrunA17_Chr5g0430781 39.860 143 68 3 31 167 28 158 1.11e-21 91.3
Msa1277210 MtrunA17_Chr8g0364881 32.639 144 72 4 32 161 4 136 1.01e-20 91.3
Msa1277210 MtrunA17_Chr6g0486651 30.942 223 117 7 6 222 21 212 1.06e-20 91.7
Msa1277210 MtrunA17_Chr4g0073141 38.793 116 62 3 48 161 78 186 1.53e-20 92.0
Msa1277210 MtrunA17_Chr4g0050041 33.333 180 104 5 34 199 58 235 1.91e-19 87.8
Msa1277210 MtrunA17_Chr8g0383861 46.835 79 39 2 94 170 113 190 5.01e-19 84.3
Msa1277210 MtrunA17_Chr8g0383881 46.835 79 39 2 94 170 103 180 5.27e-19 84.0
Msa1277210 MtrunA17_Chr6g0451161 36.957 138 71 5 33 161 71 201 6.66e-19 86.7
Msa1277210 MtrunA17_Chr5g0427431 44.186 86 47 1 81 165 121 206 6.74e-19 87.0
Msa1277210 MtrunA17_Chr1g0186661 33.824 136 75 4 40 168 53 180 7.43e-19 85.9
Msa1277210 MtrunA17_Chr3g0144241 44.737 76 42 0 94 169 79 154 7.74e-19 83.2
Msa1277210 MtrunA17_Chr8g0381731 35.514 107 57 2 93 198 147 242 8.12e-19 85.1
Msa1277210 MtrunA17_Chr4g0030831 33.548 155 81 7 22 161 7 154 9.01e-19 82.4
Msa1277210 MtrunA17_Chr1g0203541 47.297 74 33 1 94 161 105 178 1.43e-18 82.8
Msa1277210 MtrunA17_Chr1g0167811 35.461 141 79 5 34 166 34 170 1.99e-18 84.3
Msa1277210 MtrunA17_Chr8g0383941 41.935 93 50 3 80 170 94 184 2.50e-18 82.4
Msa1277210 MtrunA17_Chr3g0128771 47.222 72 35 1 94 162 91 162 2.75e-18 81.3
Msa1277210 MtrunA17_Chr7g0244851 48.101 79 38 2 91 167 78 155 3.94e-18 80.9
Msa1277210 MtrunA17_Chr2g0327791 37.600 125 66 3 64 185 86 201 5.09e-18 81.3
Msa1277210 MtrunA17_Chr7g0273051 33.333 132 72 5 40 164 44 166 5.12e-18 83.6
Msa1277210 MtrunA17_Chr3g0133001 48.000 75 38 1 94 167 130 204 5.48e-18 84.0
Msa1277210 MtrunA17_Chr3g0113811 30.573 157 90 5 21 172 17 159 5.88e-18 80.1
Msa1277210 MtrunA17_Chr2g0325161 35.669 157 86 3 16 164 19 168 7.36e-18 81.3
Msa1277210 MtrunA17_Chr8g0383871 44.304 79 41 2 94 170 112 189 9.47e-18 80.9
Msa1277210 MtrunA17_Chr8g0383921 44.304 79 41 2 94 170 111 188 1.06e-17 80.5
Msa1277210 MtrunA17_Chr3g0132491 35.915 142 68 7 30 164 57 182 1.42e-17 82.4
Msa1277210 MtrunA17_Chr8g0370171 41.250 80 44 1 94 170 70 149 1.56e-17 79.7
Msa1277210 MtrunA17_Chr3g0129671 44.318 88 44 3 94 179 109 193 1.56e-17 80.1
Msa1277210 MtrunA17_Chr6g0470751 44.944 89 45 2 94 178 97 185 1.89e-17 81.6
Msa1277210 MtrunA17_Chr1g0197031 32.836 134 79 3 34 162 18 145 2.20e-17 78.2
Msa1277210 MtrunA17_Chr3g0126931 40.230 87 46 2 83 168 95 176 2.25e-17 81.6
Msa1277210 MtrunA17_Chr5g0426881 35.252 139 68 5 30 161 62 185 2.53e-17 82.0
Msa1277210 MtrunA17_Chr3g0123631 34.783 138 62 4 31 161 1 117 2.96e-17 78.6
Msa1277210 MtrunA17_Chr3g0123641 31.429 175 85 5 31 191 1 154 3.15e-17 79.7
Msa1277210 MtrunA17_Chr5g0411081 31.387 137 89 3 32 164 58 193 3.77e-17 81.3
Msa1277210 MtrunA17_Chr2g0325141 31.655 139 69 4 39 164 11 136 3.95e-17 77.0
Msa1277210 MtrunA17_Chr1g0191601 47.368 76 35 2 94 164 98 173 4.13e-17 81.3
Msa1277210 MtrunA17_Chr7g0246761 41.463 82 38 2 82 161 59 132 7.02e-17 77.4
Msa1277210 MtrunA17_Chr2g0279431 43.038 79 44 1 84 161 125 203 8.95e-17 80.5
Msa1277210 MtrunA17_Chr4g0013561 40.196 102 54 5 93 189 84 183 1.58e-16 77.4
Msa1277210 MtrunA17_Chr5g0437361 31.858 113 62 2 58 167 51 151 3.09e-16 75.1
Msa1277210 MtrunA17_Chr2g0307411 47.059 68 34 1 94 161 77 142 1.57e-15 73.9
Msa1277210 MtrunA17_Chr8g0383931 52.830 53 24 1 118 170 140 191 1.64e-15 74.7
Msa1277210 MtrunA17_Chr8g0383901 52.830 53 24 1 118 170 130 181 1.82e-15 74.3
Msa1277210 MtrunA17_Chr1g0184171 41.892 74 41 1 94 165 103 176 2.02e-15 75.9
Msa1277210 MtrunA17_Chr7g0269491 37.589 141 67 6 72 208 47 170 3.77e-15 72.8
Msa1277210 MtrunA17_Chr4g0052671 42.105 76 41 1 94 166 78 153 8.02e-15 70.9
Msa1277210 MtrunA17_Chr1g0198201 29.412 136 85 3 28 161 4 130 1.05e-14 72.4
Msa1277210 MtrunA17_Chr3g0081611 38.961 77 44 1 89 162 75 151 1.06e-14 71.2
Msa1277210 MtrunA17_Chr5g0409211 30.405 148 83 4 20 161 3 136 1.36e-14 70.9
Msa1277210 MtrunA17_Chr3g0143301 45.333 75 39 1 94 168 48 120 2.19e-14 68.9
Msa1277210 MtrunA17_Chr5g0426871 44.444 72 36 2 94 161 108 179 2.48e-14 73.2
Msa1277210 MtrunA17_Chr7g0274741 34.409 93 53 2 94 180 70 160 3.42e-14 69.7
Msa1277210 MtrunA17_Chr8g0366561 46.667 75 37 2 93 164 113 187 3.42e-14 72.4
Msa1277210 MtrunA17_Chr1g0157091 44.118 68 36 1 94 161 48 113 5.49e-14 68.2
Msa1277210 MtrunA17_Chr6g0451051 28.667 150 80 4 33 181 4 127 5.67e-14 68.6
Msa1277210 MtrunA17_Chr8g0350741 42.647 68 37 1 96 161 64 131 7.93e-14 68.9
Msa1277210 MtrunA17_Chr1g0197021 27.660 141 95 1 23 163 10 143 8.25e-14 68.2
Msa1277210 MtrunA17_Chr2g0297011 42.466 73 39 2 94 164 76 147 8.59e-14 68.2
Msa1277210 MtrunA17_Chr5g0409201 56.250 48 21 0 115 162 89 136 8.83e-14 68.2
Msa1277210 MtrunA17_Chr4g0027761 41.096 73 41 2 94 166 100 170 1.46e-13 68.6
Msa1277210 MtrunA17_Chr8g0366521 37.234 94 46 3 94 180 76 163 5.31e-13 68.6
Msa1277210 MtrunA17_Chr8g0345401 51.064 47 23 0 119 165 49 95 7.32e-13 65.1
Msa1277210 MtrunA17_Chr6g0451031 39.130 69 42 0 100 168 50 118 7.44e-13 64.7
Msa1277210 MtrunA17_Chr7g0248841 35.897 78 41 2 94 164 74 149 8.94e-13 65.5
Msa1277210 MtrunA17_Chr7g0261091 29.452 146 78 7 31 165 15 146 1.04e-12 67.8
Msa1277210 MtrunA17_Chr7g0248831 35.616 73 44 1 93 162 79 151 1.08e-12 65.5
Msa1277210 MtrunA17_Chr8g0345381 48.148 54 28 0 119 172 53 106 1.42e-12 64.7
Msa1277210 MtrunA17_Chr8g0369571 45.455 66 36 0 119 184 90 155 1.52e-12 65.1
Msa1277210 MtrunA17_Chr7g0274711 38.356 73 41 2 94 162 67 139 1.59e-12 65.1
Msa1277210 MtrunA17_Chr5g0433861 24.268 239 153 8 31 264 27 242 1.84e-12 67.0
Msa1277210 MtrunA17_Chr3g0118061 36.000 75 45 1 91 162 73 147 2.06e-12 65.5
Msa1277210 MtrunA17_Chr1g0184631 37.838 74 40 2 94 162 68 140 2.74e-12 64.3
Msa1277210 MtrunA17_Chr8g0348151 28.261 138 93 3 29 162 22 157 2.94e-12 64.7
Msa1277210 MtrunA17_Chr7g0274721 36.765 68 43 0 94 161 68 135 3.82e-12 63.9
Msa1277210 MtrunA17_Chr8g0388201 51.111 45 22 0 119 163 347 391 3.82e-12 66.6
Msa1277210 MtrunA17_Chr5g0406031 57.778 45 19 0 117 161 187 231 3.98e-12 65.1
Msa1277210 MtrunA17_Chr2g0300331 61.364 44 17 0 118 161 168 211 4.85e-12 64.7
Msa1277210 MtrunA17_Chr7g0237871 41.333 75 40 2 107 179 144 216 5.21e-12 64.7
Msa1277210 MtrunA17_Chr3g0124531 42.683 82 38 4 118 196 68 143 5.49e-12 63.2
Msa1277210 MtrunA17_Chr3g0120011 44.444 72 36 3 92 161 92 161 9.23e-12 63.5
Msa1277210 MtrunA17_Chr3g0118071 36.486 74 44 2 91 161 73 146 1.04e-11 63.5
Msa1277210 MtrunA17_Chr4g0060981 42.857 70 38 2 94 162 149 217 1.18e-11 63.5
Msa1277210 MtrunA17_Chr8g0343571 38.095 105 59 4 100 199 62 165 1.35e-11 62.4
Msa1277210 MtrunA17_Chr4g0010171 52.000 50 24 0 116 165 110 159 1.53e-11 62.4
Msa1277210 MtrunA17_Chr1g0200721 42.254 71 37 3 94 161 208 277 2.40e-11 64.3
Msa1277210 MtrunA17_Chr5g0396611 53.333 45 21 0 119 163 340 384 3.76e-11 63.5
Msa1277210 MtrunA17_Chr5g0396601 53.333 45 21 0 119 163 350 394 5.28e-11 63.2
Msa1277210 MtrunA17_Chr2g0301871 53.191 47 22 0 118 164 82 128 5.50e-11 62.4
Msa1277210 MtrunA17_Chr7g0274731 35.294 68 44 0 94 161 89 156 7.70e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1277210 AT1G53820.1 45.695 302 127 10 26 306 19 304 8.83e-67 212
Msa1277210 AT1G72310.1 40.881 318 136 9 18 308 11 303 2.35e-66 211
Msa1277210 AT3G16720.1 37.248 298 137 13 22 313 21 274 2.35e-40 143
Msa1277210 AT3G62690.1 36.667 150 90 2 16 164 13 158 2.03e-25 102
Msa1277210 AT4G15975.1 42.241 116 55 3 49 164 18 121 8.63e-24 97.8
Msa1277210 AT5G17600.1 34.337 166 100 4 34 198 59 216 1.36e-23 99.8
Msa1277210 AT1G23980.1 36.296 135 81 3 32 161 52 186 4.31e-23 98.6
Msa1277210 AT5G05280.1 35.366 164 89 4 1 161 6 155 8.89e-23 93.6
Msa1277210 AT1G49220.1 47.170 106 45 4 72 170 84 185 1.91e-22 94.7
Msa1277210 AT3G14320.1 47.368 76 40 0 92 167 61 136 2.07e-22 93.6
Msa1277210 AT2G47560.1 36.842 152 82 3 16 164 13 153 7.95e-22 92.4
Msa1277210 AT2G20030.1 39.437 142 72 4 27 167 44 172 3.73e-21 93.6
Msa1277210 AT1G04360.1 35.570 149 71 6 31 168 49 183 4.43e-21 93.2
Msa1277210 AT2G42360.1 34.932 146 80 2 21 164 21 153 4.74e-21 90.5
Msa1277210 AT5G43420.1 31.220 205 105 7 20 202 31 221 2.85e-20 90.9
Msa1277210 AT3G48030.2 34.459 148 92 3 31 175 41 186 4.31e-20 88.6
Msa1277210 AT1G20823.1 33.766 154 85 5 21 169 20 161 4.45e-20 87.0
Msa1277210 AT1G49210.1 46.154 91 46 2 94 182 108 197 5.21e-20 87.4
Msa1277210 AT1G49200.1 48.810 84 40 2 89 170 104 186 5.99e-20 87.4
Msa1277210 AT3G48030.1 34.459 148 92 3 31 175 119 264 9.28e-20 89.0
Msa1277210 AT2G35000.1 30.045 223 135 7 32 244 51 262 1.51e-19 88.6
Msa1277210 AT4G40070.1 36.000 150 77 6 28 171 40 176 1.69e-19 87.8
Msa1277210 AT3G18773.1 50.633 79 36 2 94 170 103 180 1.98e-19 85.9
Msa1277210 AT4G28890.1 44.330 97 50 2 94 189 97 190 2.55e-19 88.6
Msa1277210 AT5G05810.1 52.000 75 35 1 94 167 120 194 3.01e-19 88.2
Msa1277210 AT1G76410.1 33.571 140 78 4 32 169 28 154 3.46e-19 84.3
Msa1277210 AT5G10380.1 46.067 89 47 1 93 180 108 196 6.97e-19 85.9
Msa1277210 AT4G30400.1 42.157 102 52 3 65 161 77 176 1.60e-18 86.3
Msa1277210 AT5G40250.1 34.014 147 73 4 33 161 45 185 3.10e-18 84.7
Msa1277210 AT1G72200.1 31.765 170 98 4 2 166 36 192 3.40e-18 85.1
Msa1277210 AT4G10150.1 28.415 183 118 3 32 203 26 206 6.04e-18 82.0
Msa1277210 AT3G10910.1 44.086 93 48 3 94 184 88 178 7.38e-18 80.5
Msa1277210 AT3G03550.1 46.667 75 39 1 95 168 134 208 9.87e-18 83.2
Msa1277210 AT2G18650.1 45.977 87 44 3 82 165 87 173 1.60e-17 83.2
Msa1277210 AT5G01880.1 45.833 72 38 1 92 162 77 148 2.02e-17 78.6
Msa1277210 AT3G19140.1 41.176 85 44 1 83 161 37 121 2.03e-17 78.2
Msa1277210 AT2G27940.1 31.343 134 89 1 32 162 50 183 6.05e-17 79.3
Msa1277210 AT1G33480.1 40.698 86 48 1 76 161 60 142 8.30e-17 79.3
Msa1277210 AT5G42200.1 31.544 149 80 4 19 161 14 146 1.06e-16 76.6
Msa1277210 AT2G17450.1 40.777 103 48 3 75 169 55 152 1.09e-16 77.4
Msa1277210 AT4G10160.1 42.857 70 40 0 92 161 70 139 1.20e-16 78.2
Msa1277210 AT4G09110.1 32.374 139 72 3 33 167 51 171 1.61e-16 79.0
Msa1277210 AT4G33565.1 38.318 107 64 2 84 189 181 286 1.68e-16 79.7
Msa1277210 AT4G17905.1 44.186 86 45 2 84 166 117 202 2.02e-16 79.0
Msa1277210 AT1G72220.1 29.861 144 94 3 25 166 86 224 2.09e-16 79.7
Msa1277210 AT4G09120.1 38.144 97 55 2 75 167 76 171 2.11e-16 79.3
Msa1277210 AT4G17905.2 44.186 86 45 2 84 166 143 228 2.22e-16 79.0
Msa1277210 AT2G42350.1 30.496 141 82 1 21 161 18 142 4.45e-16 76.3
Msa1277210 AT1G49230.1 44.304 79 41 2 94 170 104 181 5.45e-16 76.3
Msa1277210 AT1G32361.1 36.364 99 56 3 69 167 111 202 5.52e-16 77.8
Msa1277210 AT5G27420.1 41.463 82 44 2 94 171 95 176 5.59e-16 78.2
Msa1277210 AT2G34990.2 43.056 72 37 1 94 161 89 160 8.63e-16 77.4
Msa1277210 AT5G57750.1 30.464 151 86 5 21 162 25 165 9.01e-16 75.5
Msa1277210 AT2G34990.1 43.056 72 37 1 94 161 67 138 9.65e-16 77.0
Msa1277210 AT4G09130.1 37.363 91 53 2 82 168 79 169 1.63e-15 76.6
Msa1277210 AT1G74410.1 42.500 80 44 2 87 164 142 221 1.70e-15 74.7
Msa1277210 AT3G05200.1 38.889 90 46 3 94 174 99 188 2.17e-15 76.6
Msa1277210 AT5G07040.1 39.189 74 42 1 92 162 64 137 2.41e-15 72.8
Msa1277210 AT2G35420.1 32.031 128 77 4 37 161 25 145 3.46e-15 74.7
Msa1277210 AT5G58580.1 38.393 112 62 3 94 198 111 222 3.48e-15 75.5
Msa1277210 AT4G17920.1 45.833 72 35 1 94 161 81 152 6.35e-15 74.3
Msa1277210 AT3G18930.2 36.559 93 47 2 84 164 109 201 1.28e-14 74.3
Msa1277210 AT3G18930.3 36.559 93 47 2 84 164 109 201 1.28e-14 74.3
Msa1277210 AT3G18930.1 36.559 93 47 2 84 164 109 201 1.28e-14 74.3
Msa1277210 AT1G22500.1 36.441 118 63 4 80 192 75 185 1.31e-14 74.3
Msa1277210 AT4G09100.1 44.000 75 38 1 94 164 56 130 3.66e-14 68.9
Msa1277210 AT5G46650.1 34.507 142 72 7 32 161 25 157 4.45e-14 72.0
Msa1277210 AT1G35330.1 41.667 72 38 1 94 161 100 171 9.81e-14 71.2
Msa1277210 AT3G61550.1 31.034 116 60 4 60 162 71 179 3.60e-13 68.2
Msa1277210 AT4G35480.1 34.737 95 59 1 112 206 106 197 8.15e-13 66.6
Msa1277210 AT3G60220.1 38.372 86 46 2 84 162 75 160 1.09e-12 68.2
Msa1277210 AT3G11110.1 30.000 150 88 3 21 168 22 156 1.51e-12 65.1
Msa1277210 AT2G46493.1 45.833 48 26 0 115 162 134 181 1.55e-12 65.9
Msa1277210 AT2G46160.1 30.612 98 57 2 76 162 84 181 2.13e-12 65.9
Msa1277210 AT2G46160.2 30.612 98 57 2 76 162 84 181 2.13e-12 65.9
Msa1277210 AT5G06490.1 34.615 78 43 1 93 162 95 172 3.00e-12 65.1
Msa1277210 AT2G44578.1 58.140 43 18 0 119 161 70 112 5.80e-12 63.2
Msa1277210 AT5G66070.5 54.348 46 21 0 117 162 174 219 7.24e-12 64.3
Msa1277210 AT5G66070.1 54.348 46 21 0 117 162 174 219 7.24e-12 64.3
Msa1277210 AT2G46494.1 47.917 48 25 0 115 162 312 359 7.33e-12 65.9
Msa1277210 AT4G24015.1 42.500 80 42 3 93 170 79 156 8.01e-12 63.5
Msa1277210 AT2G17730.1 40.278 72 39 1 94 161 167 238 9.86e-12 64.3
Msa1277210 AT5G53110.1 36.735 98 54 3 73 162 276 373 1.00e-11 65.9
Msa1277210 AT5G66070.2 55.556 45 20 0 117 161 198 242 1.20e-11 64.3
Msa1277210 AT2G34000.1 34.343 99 57 3 73 163 37 135 1.30e-11 62.4
Msa1277210 AT1G28040.1 45.833 48 26 0 115 162 249 296 2.07e-11 64.3
Msa1277210 AT5G47610.1 41.270 63 35 1 104 164 90 152 2.14e-11 62.0
Msa1277210 AT3G60966.1 60.465 43 17 0 119 161 62 104 6.70e-11 60.1
Msa1277210 AT2G44581.1 53.488 43 20 0 119 161 70 112 6.82e-11 60.1
Msa1277210 AT3G20395.1 36.111 72 45 1 94 165 147 217 7.02e-11 61.6
Msa1277210 AT2G46495.2 45.833 48 26 0 115 162 288 335 8.38e-11 62.8
Msa1277210 AT2G46495.1 45.833 48 26 0 115 162 316 363 8.67e-11 62.8

Find 55 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCAAGATCCTGTTGTTTA+TGG 0.168039 8_3:+12417267 Msa1277210:CDS
TCCTACTAATGTGTTGGTTT+GGG 0.185611 8_3:+12417635 Msa1277210:CDS
TCTTAAGTCTTTGCCTGTTT+TGG 0.218884 8_3:+12417320 Msa1277210:CDS
TGAAACCCATGATTGCATTT+TGG 0.226368 8_3:-12417429 None:intergenic
CGTCGACGACGGCGGTTTCT+TGG 0.237232 8_3:-12417225 None:intergenic
TTTGAAGCAGTAGTACTATT+TGG 0.249796 8_3:-12417534 None:intergenic
ATTCTACTTGTCCTCTTTGT+AGG 0.257676 8_3:+12417484 Msa1277210:CDS
TTCCTACTAATGTGTTGGTT+TGG 0.265728 8_3:+12417634 Msa1277210:CDS
ATTTATATGCAAAGTGGTTT+TGG 0.278266 8_3:+12417172 Msa1277210:CDS
CATTGTCACCTTCTGATAAT+AGG 0.288696 8_3:+12417844 Msa1277210:CDS
TCCTTCAACGCCAAGTTCTA+TGG 0.312910 8_3:+12417950 Msa1277210:CDS
GTTTATGGAAGTCACCAAAT+TGG 0.322819 8_3:+12417282 Msa1277210:CDS
ACACAAGTTAGTTCTTCTTT+TGG 0.347982 8_3:+12417669 Msa1277210:CDS
TGAGGATTTCAAAGATGGTT+TGG 0.348325 8_3:+12417353 Msa1277210:CDS
AAAGGGTCAGGTGATGTATT+AGG 0.362982 8_3:+12417060 Msa1277210:CDS
GATGATGACGATGTTGAAAA+AGG 0.389069 8_3:-12417726 None:intergenic
GCAACTGCAACTGCAACAAA+AGG 0.394252 8_3:+12417042 Msa1277210:CDS
AAGAATGAGGATTTCAAAGA+TGG 0.398458 8_3:+12417348 Msa1277210:CDS
TCTAATACAGTTGAAATAAC+AGG 0.400393 8_3:+12417087 Msa1277210:CDS
CAAGATTGAGGTCATTTAAG+AGG 0.400414 8_3:+12417901 Msa1277210:CDS
TCCATAGAACTTGGCGTTGA+AGG 0.401246 8_3:-12417951 None:intergenic
AGTAGTAGATGTCATGGAAA+TGG 0.412403 8_3:+12417774 Msa1277210:CDS
TGTCTTTGTGATGTTGTTGA+AGG 0.438454 8_3:+12417387 Msa1277210:CDS
TCATTCTTGAAAACCAAAAC+AGG 0.442504 8_3:-12417333 None:intergenic
ATTTAGATTGTATTGATATG+TGG 0.449698 8_3:+12417451 Msa1277210:CDS
TGGTGACTTCCATAAACAAC+AGG 0.454316 8_3:-12417276 None:intergenic
CGACGGCGGTTTCTTGGTTG+AGG 0.455007 8_3:-12417219 None:intergenic
CCTACTAATGTGTTGGTTTG+GGG 0.459901 8_3:+12417636 Msa1277210:CDS
GACCTCAATCTTGTTGTCAT+TGG 0.460150 8_3:-12417891 None:intergenic
CCCCAAACCAACACATTAGT+AGG 0.471494 8_3:-12417636 None:intergenic
TTTGGTGTTTCTTTGGAAGA+AGG 0.471878 8_3:+12417687 Msa1277210:CDS
TTAGAGGTCACTACTAGCTC+TGG 0.483042 8_3:-12417986 None:intergenic
CTACTAATGTGTTGGTTTGG+GGG 0.498156 8_3:+12417637 Msa1277210:CDS
GAACTAAATTCACTTGGTAA+TGG 0.506647 8_3:-12417816 None:intergenic
GAAAATTTGAGTCATGTGAA+TGG 0.511787 8_3:+12417594 Msa1277210:CDS
TGTTCCACATCCATAGAACT+TGG 0.513956 8_3:-12417960 None:intergenic
AATAGTACTACTGCTTCAAA+TGG 0.515388 8_3:+12417537 Msa1277210:CDS
GTTAATAGTAGTAGATGTCA+TGG 0.517473 8_3:+12417768 Msa1277210:CDS
AACGCCAAGTTCTATGGATG+TGG 0.519885 8_3:+12417956 Msa1277210:CDS
AAACAAACCGCCGTCGACGA+CGG 0.534947 8_3:-12417236 None:intergenic
TGCTTCATTTATATGCAAAG+TGG 0.539115 8_3:+12417166 Msa1277210:CDS
GCAACTGCAACAAAAGGGTC+AGG 0.546145 8_3:+12417048 Msa1277210:CDS
CAACTGCAACTGCAACAAAA+GGG 0.548726 8_3:+12417043 Msa1277210:CDS
CACCAATGACAACAAGATTG+AGG 0.562761 8_3:+12417889 Msa1277210:CDS
GAAACTAAATTCCTACAAAG+AGG 0.564972 8_3:-12417495 None:intergenic
CAAGAAACCGCCGTCGTCGA+CGG 0.576823 8_3:+12417226 Msa1277210:CDS
AGTTCTATGGATGTGGAACA+AGG 0.581073 8_3:+12417963 Msa1277210:CDS
ATGTCATGGAAATGGAATGT+TGG 0.602451 8_3:+12417782 Msa1277210:CDS
GAGGAAGAACTAAATTCACT+TGG 0.635097 8_3:-12417822 None:intergenic
TCAACACACCTATTATCAGA+AGG 0.647870 8_3:-12417852 None:intergenic
CAAACCGCCGTCGACGACGG+CGG 0.650391 8_3:-12417233 None:intergenic
GAAACCGCCGTCGTCGACGG+CGG 0.652134 8_3:+12417229 Msa1277210:CDS
ATTATCAGAAGGTGACAATG+AGG 0.655023 8_3:-12417841 None:intergenic
TAAACAACAGGATCTTGACC+AGG 0.667684 8_3:-12417264 None:intergenic
AATGTGTTAGTGTCATCAGA+AGG 0.695473 8_3:+12417570 Msa1277210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAATAACAGGAAAAATTA+TGG + chr8_3:12417098-12417117 Msa1277210:CDS 20.0%
!!! AGCAATAATTTTTCTATTCA+TGG + chr8_3:12417125-12417144 Msa1277210:CDS 20.0%
!!! ATTTAGATTGTATTGATATG+TGG + chr8_3:12417451-12417470 Msa1277210:CDS 20.0%
!! TGAAATAACAGGAAAAATTA+TGG + chr8_3:12417098-12417117 Msa1277210:CDS 20.0%
!!! AGCAATAATTTTTCTATTCA+TGG + chr8_3:12417125-12417144 Msa1277210:CDS 20.0%
!!! ATTTAGATTGTATTGATATG+TGG + chr8_3:12417451-12417470 Msa1277210:CDS 20.0%
! TCTAATACAGTTGAAATAAC+AGG + chr8_3:12417087-12417106 Msa1277210:CDS 25.0%
!! ATTTATATGCAAAGTGGTTT+TGG + chr8_3:12417172-12417191 Msa1277210:CDS 25.0%
!! TTTTACCAAAATGCAATCAT+GGG + chr8_3:12417424-12417443 Msa1277210:CDS 25.0%
!! AAATTTTCCTACTAATGTGT+TGG + chr8_3:12417629-12417648 Msa1277210:CDS 25.0%
! TCTAATACAGTTGAAATAAC+AGG + chr8_3:12417087-12417106 Msa1277210:CDS 25.0%
!! ATTTATATGCAAAGTGGTTT+TGG + chr8_3:12417172-12417191 Msa1277210:CDS 25.0%
!! TTTTACCAAAATGCAATCAT+GGG + chr8_3:12417424-12417443 Msa1277210:CDS 25.0%
!! AAATTTTCCTACTAATGTGT+TGG + chr8_3:12417629-12417648 Msa1277210:CDS 25.0%
TGCTTCATTTATATGCAAAG+TGG + chr8_3:12417166-12417185 Msa1277210:CDS 30.0%
!!! TTTTAGCTTCAAGTCCAATT+TGG - chr8_3:12417299-12417318 None:intergenic 30.0%
TCATTCTTGAAAACCAAAAC+AGG - chr8_3:12417336-12417355 None:intergenic 30.0%
!!! TGTTTTGGTTTTCAAGAATG+AGG + chr8_3:12417335-12417354 Msa1277210:CDS 30.0%
AAGAATGAGGATTTCAAAGA+TGG + chr8_3:12417348-12417367 Msa1277210:CDS 30.0%
TTGTTGAAGGAGAAAAAACT+AGG + chr8_3:12417400-12417419 Msa1277210:CDS 30.0%
! CTTTTACCAAAATGCAATCA+TGG + chr8_3:12417423-12417442 Msa1277210:CDS 30.0%
GAAACTAAATTCCTACAAAG+AGG - chr8_3:12417498-12417517 None:intergenic 30.0%
! TTTGAAGCAGTAGTACTATT+TGG - chr8_3:12417537-12417556 None:intergenic 30.0%
AATAGTACTACTGCTTCAAA+TGG + chr8_3:12417537-12417556 Msa1277210:CDS 30.0%
GAAAATTTGAGTCATGTGAA+TGG + chr8_3:12417594-12417613 Msa1277210:CDS 30.0%
!! ACACAAGTTAGTTCTTCTTT+TGG + chr8_3:12417669-12417688 Msa1277210:CDS 30.0%
!!! TTCTTCTTTTGGTGTTTCTT+TGG + chr8_3:12417680-12417699 Msa1277210:CDS 30.0%
GTTAATAGTAGTAGATGTCA+TGG + chr8_3:12417768-12417787 Msa1277210:CDS 30.0%
GAACTAAATTCACTTGGTAA+TGG - chr8_3:12417819-12417838 None:intergenic 30.0%
TGCTTCATTTATATGCAAAG+TGG + chr8_3:12417166-12417185 Msa1277210:CDS 30.0%
!!! TTTTAGCTTCAAGTCCAATT+TGG - chr8_3:12417299-12417318 None:intergenic 30.0%
TCATTCTTGAAAACCAAAAC+AGG - chr8_3:12417336-12417355 None:intergenic 30.0%
!!! TGTTTTGGTTTTCAAGAATG+AGG + chr8_3:12417335-12417354 Msa1277210:CDS 30.0%
AAGAATGAGGATTTCAAAGA+TGG + chr8_3:12417348-12417367 Msa1277210:CDS 30.0%
TTGTTGAAGGAGAAAAAACT+AGG + chr8_3:12417400-12417419 Msa1277210:CDS 30.0%
! CTTTTACCAAAATGCAATCA+TGG + chr8_3:12417423-12417442 Msa1277210:CDS 30.0%
GAAACTAAATTCCTACAAAG+AGG - chr8_3:12417498-12417517 None:intergenic 30.0%
! TTTGAAGCAGTAGTACTATT+TGG - chr8_3:12417537-12417556 None:intergenic 30.0%
AATAGTACTACTGCTTCAAA+TGG + chr8_3:12417537-12417556 Msa1277210:CDS 30.0%
GAAAATTTGAGTCATGTGAA+TGG + chr8_3:12417594-12417613 Msa1277210:CDS 30.0%
!! ACACAAGTTAGTTCTTCTTT+TGG + chr8_3:12417669-12417688 Msa1277210:CDS 30.0%
!!! TTCTTCTTTTGGTGTTTCTT+TGG + chr8_3:12417680-12417699 Msa1277210:CDS 30.0%
GTTAATAGTAGTAGATGTCA+TGG + chr8_3:12417768-12417787 Msa1277210:CDS 30.0%
GAACTAAATTCACTTGGTAA+TGG - chr8_3:12417819-12417838 None:intergenic 30.0%
!!! TATATGCAAAGTGGTTTTGG+TGG + chr8_3:12417175-12417194 Msa1277210:CDS 35.0%
GTTTATGGAAGTCACCAAAT+TGG + chr8_3:12417282-12417301 Msa1277210:CDS 35.0%
! TCTTAAGTCTTTGCCTGTTT+TGG + chr8_3:12417320-12417339 Msa1277210:CDS 35.0%
TGAGGATTTCAAAGATGGTT+TGG + chr8_3:12417353-12417372 Msa1277210:CDS 35.0%
TGTCTTTGTGATGTTGTTGA+AGG + chr8_3:12417387-12417406 Msa1277210:CDS 35.0%
! TGAAACCCATGATTGCATTT+TGG - chr8_3:12417432-12417451 None:intergenic 35.0%
ATTCTACTTGTCCTCTTTGT+AGG + chr8_3:12417484-12417503 Msa1277210:CDS 35.0%
AATGTGTTAGTGTCATCAGA+AGG + chr8_3:12417570-12417589 Msa1277210:CDS 35.0%
!! TTCCTACTAATGTGTTGGTT+TGG + chr8_3:12417634-12417653 Msa1277210:CDS 35.0%
!! TCCTACTAATGTGTTGGTTT+GGG + chr8_3:12417635-12417654 Msa1277210:CDS 35.0%
!! TTTGGTGTTTCTTTGGAAGA+AGG + chr8_3:12417687-12417706 Msa1277210:CDS 35.0%
! GATGATGACGATGTTGAAAA+AGG - chr8_3:12417729-12417748 None:intergenic 35.0%
AGTAGTAGATGTCATGGAAA+TGG + chr8_3:12417774-12417793 Msa1277210:CDS 35.0%
ATGTCATGGAAATGGAATGT+TGG + chr8_3:12417782-12417801 Msa1277210:CDS 35.0%
GAGGAAGAACTAAATTCACT+TGG - chr8_3:12417825-12417844 None:intergenic 35.0%
ATTATCAGAAGGTGACAATG+AGG - chr8_3:12417844-12417863 None:intergenic 35.0%
CATTGTCACCTTCTGATAAT+AGG + chr8_3:12417844-12417863 Msa1277210:CDS 35.0%
TCAACACACCTATTATCAGA+AGG - chr8_3:12417855-12417874 None:intergenic 35.0%
CAAGATTGAGGTCATTTAAG+AGG + chr8_3:12417901-12417920 Msa1277210:CDS 35.0%
!!! TATATGCAAAGTGGTTTTGG+TGG + chr8_3:12417175-12417194 Msa1277210:CDS 35.0%
GTTTATGGAAGTCACCAAAT+TGG + chr8_3:12417282-12417301 Msa1277210:CDS 35.0%
! TCTTAAGTCTTTGCCTGTTT+TGG + chr8_3:12417320-12417339 Msa1277210:CDS 35.0%
TGAGGATTTCAAAGATGGTT+TGG + chr8_3:12417353-12417372 Msa1277210:CDS 35.0%
TGTCTTTGTGATGTTGTTGA+AGG + chr8_3:12417387-12417406 Msa1277210:CDS 35.0%
! TGAAACCCATGATTGCATTT+TGG - chr8_3:12417432-12417451 None:intergenic 35.0%
ATTCTACTTGTCCTCTTTGT+AGG + chr8_3:12417484-12417503 Msa1277210:CDS 35.0%
AATGTGTTAGTGTCATCAGA+AGG + chr8_3:12417570-12417589 Msa1277210:CDS 35.0%
!! TTCCTACTAATGTGTTGGTT+TGG + chr8_3:12417634-12417653 Msa1277210:CDS 35.0%
!! TCCTACTAATGTGTTGGTTT+GGG + chr8_3:12417635-12417654 Msa1277210:CDS 35.0%
!! TTTGGTGTTTCTTTGGAAGA+AGG + chr8_3:12417687-12417706 Msa1277210:CDS 35.0%
! GATGATGACGATGTTGAAAA+AGG - chr8_3:12417729-12417748 None:intergenic 35.0%
AGTAGTAGATGTCATGGAAA+TGG + chr8_3:12417774-12417793 Msa1277210:CDS 35.0%
ATGTCATGGAAATGGAATGT+TGG + chr8_3:12417782-12417801 Msa1277210:CDS 35.0%
GAGGAAGAACTAAATTCACT+TGG - chr8_3:12417825-12417844 None:intergenic 35.0%
ATTATCAGAAGGTGACAATG+AGG - chr8_3:12417844-12417863 None:intergenic 35.0%
CATTGTCACCTTCTGATAAT+AGG + chr8_3:12417844-12417863 Msa1277210:CDS 35.0%
TCAACACACCTATTATCAGA+AGG - chr8_3:12417855-12417874 None:intergenic 35.0%
CAAGATTGAGGTCATTTAAG+AGG + chr8_3:12417901-12417920 Msa1277210:CDS 35.0%
CAACTGCAACTGCAACAAAA+GGG + chr8_3:12417043-12417062 Msa1277210:CDS 40.0%
AAAGGGTCAGGTGATGTATT+AGG + chr8_3:12417060-12417079 Msa1277210:CDS 40.0%
TAAACAACAGGATCTTGACC+AGG - chr8_3:12417267-12417286 None:intergenic 40.0%
! GGTCAAGATCCTGTTGTTTA+TGG + chr8_3:12417267-12417286 Msa1277210:CDS 40.0%
TGGTGACTTCCATAAACAAC+AGG - chr8_3:12417279-12417298 None:intergenic 40.0%
!! CCTACTAATGTGTTGGTTTG+GGG + chr8_3:12417636-12417655 Msa1277210:CDS 40.0%
!! CTACTAATGTGTTGGTTTGG+GGG + chr8_3:12417637-12417656 Msa1277210:CDS 40.0%
CACCAATGACAACAAGATTG+AGG + chr8_3:12417889-12417908 Msa1277210:CDS 40.0%
! GACCTCAATCTTGTTGTCAT+TGG - chr8_3:12417894-12417913 None:intergenic 40.0%
TGTTCCACATCCATAGAACT+TGG - chr8_3:12417963-12417982 None:intergenic 40.0%
AGTTCTATGGATGTGGAACA+AGG + chr8_3:12417963-12417982 Msa1277210:CDS 40.0%
CAACTGCAACTGCAACAAAA+GGG + chr8_3:12417043-12417062 Msa1277210:CDS 40.0%
AAAGGGTCAGGTGATGTATT+AGG + chr8_3:12417060-12417079 Msa1277210:CDS 40.0%
TAAACAACAGGATCTTGACC+AGG - chr8_3:12417267-12417286 None:intergenic 40.0%
! GGTCAAGATCCTGTTGTTTA+TGG + chr8_3:12417267-12417286 Msa1277210:CDS 40.0%
TGGTGACTTCCATAAACAAC+AGG - chr8_3:12417279-12417298 None:intergenic 40.0%
!! CCTACTAATGTGTTGGTTTG+GGG + chr8_3:12417636-12417655 Msa1277210:CDS 40.0%
!! CTACTAATGTGTTGGTTTGG+GGG + chr8_3:12417637-12417656 Msa1277210:CDS 40.0%
CACCAATGACAACAAGATTG+AGG + chr8_3:12417889-12417908 Msa1277210:CDS 40.0%
! GACCTCAATCTTGTTGTCAT+TGG - chr8_3:12417894-12417913 None:intergenic 40.0%
TGTTCCACATCCATAGAACT+TGG - chr8_3:12417963-12417982 None:intergenic 40.0%
AGTTCTATGGATGTGGAACA+AGG + chr8_3:12417963-12417982 Msa1277210:CDS 40.0%
GCAACTGCAACTGCAACAAA+AGG + chr8_3:12417042-12417061 Msa1277210:CDS 45.0%
CCCCAAACCAACACATTAGT+AGG - chr8_3:12417639-12417658 None:intergenic 45.0%
TCCTTCAACGCCAAGTTCTA+TGG + chr8_3:12417950-12417969 Msa1277210:CDS 45.0%
! TCCATAGAACTTGGCGTTGA+AGG - chr8_3:12417954-12417973 None:intergenic 45.0%
AACGCCAAGTTCTATGGATG+TGG + chr8_3:12417956-12417975 Msa1277210:CDS 45.0%
GCAACTGCAACTGCAACAAA+AGG + chr8_3:12417042-12417061 Msa1277210:CDS 45.0%
CCCCAAACCAACACATTAGT+AGG - chr8_3:12417639-12417658 None:intergenic 45.0%
TCCTTCAACGCCAAGTTCTA+TGG + chr8_3:12417950-12417969 Msa1277210:CDS 45.0%
! TCCATAGAACTTGGCGTTGA+AGG - chr8_3:12417954-12417973 None:intergenic 45.0%
AACGCCAAGTTCTATGGATG+TGG + chr8_3:12417956-12417975 Msa1277210:CDS 45.0%
GCAACTGCAACAAAAGGGTC+AGG + chr8_3:12417048-12417067 Msa1277210:CDS 50.0%
!!! GTGGTTTTGGTGGCGTTTTG+AGG + chr8_3:12417185-12417204 Msa1277210:CDS 50.0%
GCAACTGCAACAAAAGGGTC+AGG + chr8_3:12417048-12417067 Msa1277210:CDS 50.0%
!!! GTGGTTTTGGTGGCGTTTTG+AGG + chr8_3:12417185-12417204 Msa1277210:CDS 50.0%
AAACAAACCGCCGTCGACGA+CGG - chr8_3:12417239-12417258 None:intergenic 55.0%
!!! CGGCGGTTTGTTTTTGCTCC+TGG + chr8_3:12417246-12417265 Msa1277210:CDS 55.0%
AAACAAACCGCCGTCGACGA+CGG - chr8_3:12417239-12417258 None:intergenic 55.0%
!!! CGGCGGTTTGTTTTTGCTCC+TGG + chr8_3:12417246-12417265 Msa1277210:CDS 55.0%
CGACGGCGGTTTCTTGGTTG+AGG - chr8_3:12417222-12417241 None:intergenic 60.0%
CAAGAAACCGCCGTCGTCGA+CGG + chr8_3:12417226-12417245 Msa1277210:CDS 60.0%
CGACGGCGGTTTCTTGGTTG+AGG - chr8_3:12417222-12417241 None:intergenic 60.0%
CAAGAAACCGCCGTCGTCGA+CGG + chr8_3:12417226-12417245 Msa1277210:CDS 60.0%
CGTCGACGACGGCGGTTTCT+TGG - chr8_3:12417228-12417247 None:intergenic 65.0%
CGTCGACGACGGCGGTTTCT+TGG - chr8_3:12417228-12417247 None:intergenic 65.0%
GAAACCGCCGTCGTCGACGG+CGG + chr8_3:12417229-12417248 Msa1277210:CDS 70.0%
CAAACCGCCGTCGACGACGG+CGG - chr8_3:12417236-12417255 None:intergenic 70.0%
GAAACCGCCGTCGTCGACGG+CGG + chr8_3:12417229-12417248 Msa1277210:CDS 70.0%
CAAACCGCCGTCGACGACGG+CGG - chr8_3:12417236-12417255 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 12417024 12417995 12417024 ID=Msa1277210;Name=Msa1277210
chr8_3 mRNA 12417024 12417995 12417024 ID=Msa1277210-mRNA-1;Parent=Msa1277210;Name=Msa1277210-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|-1|0|1|-1|1|1|0|323
chr8_3 exon 12417024 12417995 12417024 ID=Msa1277210-mRNA-1:exon:5025;Parent=Msa1277210-mRNA-1
chr8_3 CDS 12417024 12417995 12417024 ID=Msa1277210-mRNA-1:cds;Parent=Msa1277210-mRNA-1
Gene Sequence

>Msa1277210

ATGGGTGCAACTGCAGCTGCAACTGCAACTGCAACAAAAGGGTCAGGTGATGTATTAGGTGATTCTAATACAGTTGAAATAACAGGAAAAATTATGGTTTTAGCAATAATTTTTCTATTCATGGTTGTTATGTTTGTTCTAATGCTTCATTTATATGCAAAGTGGTTTTGGTGGCGTTTTGAGGAAAGAACTGTTCCTCAACCAAGAAACCGCCGTCGTCGACGGCGGTTTGTTTTTGCTCCTGGTCAAGATCCTGTTGTTTATGGAAGTCACCAAATTGGACTTGAAGCTAAAATTCTTAAGTCTTTGCCTGTTTTGGTTTTCAAGAATGAGGATTTCAAAGATGGTTTGGAATGTGCTGTTTGTCTTTGTGATGTTGTTGAAGGAGAAAAAACTAGGCTTTTACCAAAATGCAATCATGGGTTTCATTTAGATTGTATTGATATGTGGTTTCAATCTCATTCTACTTGTCCTCTTTGTAGGAATTTAGTTTCTGTTGAATCATGTAAACCAAATAGTACTACTGCTTCAAATGGTGAAGAGATGAATGTGTTAGTGTCATCAGAAGGTGAAAATTTGAGTCATGTGAATGGTTTAGAGTCTTCAAATTTTCCTACTAATGTGTTGGTTTGGGGGAATTCTCAAACACAAGTTAGTTCTTCTTTTGGTGTTTCTTTGGAAGAAGGAAGTTCTCATCAACAACCTTTTTCAACATCGTCATCATCATCTTTAGATAGTGATAATGTTAATAGTAGTAGATGTCATGGAAATGGAATGTTGGTGATTGATATACCATTACCAAGTGAATTTAGTTCTTCCTCATTGTCACCTTCTGATAATAGGTGTGTTGAAGATGAGATGAAATCACCAATGACAACAAGATTGAGGTCATTTAAGAGGCTTTTAAGTAGAGACAAAAAGTTAAGTCCTTCAACGCCAAGTTCTATGGATGTGGAACAAGGCCAGAGCTAG
Protein sequence

>Msa1277210

MGATAAATATATKGSGDVLGDSNTVEITGKIMVLAIIFLFMVVMFVLMLHLYAKWFWWRFEERTVPQPRNRRRRRRFVFAPGQDPVVYGSHQIGLEAKILKSLPVLVFKNEDFKDGLECAVCLCDVVEGEKTRLLPKCNHGFHLDCIDMWFQSHSTCPLCRNLVSVESCKPNSTTASNGEEMNVLVSSEGENLSHVNGLESSNFPTNVLVWGNSQTQVSSSFGVSLEEGSSHQQPFSTSSSSSLDSDNVNSSRCHGNGMLVIDIPLPSEFSSSSLSPSDNRCVEDEMKSPMTTRLRSFKRLLSRDKKLSPSTPSSMDVEQGQS*