AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1278670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1278670 MtrunA17_Chr8g0348581 86.275 255 31 4 1 252 1 254 5.54e-142 398
Msa1278670 MtrunA17_Chr8g0348571 91.333 150 10 3 1 149 1 148 4.83e-92 270
Msa1278670 MtrunA17_Chr3g0083791 62.346 162 42 4 1 154 1 151 5.91e-58 181
Msa1278670 MtrunA17_Chr8g0348601 54.626 227 65 12 20 238 3 199 5.80e-57 180
Msa1278670 MtrunA17_Chr3g0083161 61.728 162 43 4 1 154 1 151 7.30e-57 178
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1278670 AT4G00310.2 30.284 317 141 14 1 252 1 302 1.55e-16 77.8
Msa1278670 AT4G00310.1 30.284 317 141 14 1 252 1 302 1.55e-16 77.8

Find 80 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGAATTCTATTATTATTC+TGG 0.162225 8_3:+15135421 Msa1278670:CDS
TGTTGTATTGTTTGTTCTTA+TGG 0.227248 8_3:+15135799 Msa1278670:three_prime_UTR
TTCTATTATTATTCTGGGTT+TGG 0.245234 8_3:+15135427 Msa1278670:CDS
TCATCTTCACTTCTACAAAA+TGG 0.281057 8_3:+15134649 Msa1278670:CDS
TCATTGGTGGGTTCGGCTTC+GGG 0.282884 8_3:+15135388 Msa1278670:CDS
GTCATTGGTGGGTTCGGCTT+CGG 0.286926 8_3:+15135387 Msa1278670:CDS
AACAAGAGTGTTGTGGAATT+AGG 0.304116 8_3:+15135502 Msa1278670:CDS
GATGATTCTGGTGATGATAA+TGG 0.323884 8_3:+15135649 Msa1278670:CDS
GAGGATGATTATGATGATTC+TGG 0.345855 8_3:+15135637 Msa1278670:CDS
TTCACAAATGGGGATTCCTT+TGG 0.354696 8_3:+15134992 Msa1278670:CDS
GAAGGGATATTTATATAGAT+AGG 0.367782 8_3:+15135724 Msa1278670:three_prime_UTR
GGAGGAGTTGTTGGAGGCAA+AGG 0.371296 8_3:-15134740 None:intergenic
AATGGTGTTTGATGATAATA+AGG 0.374369 8_3:+15135264 Msa1278670:CDS
ATGAATTCTATTATTATTCT+GGG 0.377468 8_3:+15135422 Msa1278670:CDS
GAGGAGTTGTTGGAGGCAAA+GGG 0.388818 8_3:-15134739 None:intergenic
ATATTTATATAGATAGGGTT+GGG 0.397118 8_3:+15135730 Msa1278670:three_prime_UTR
TTGTGAAAATATCTTGCTCT+TGG 0.399145 8_3:-15134976 None:intergenic
AATAAGGTGGTTGATAATAA+TGG 0.414023 8_3:+15135280 Msa1278670:CDS
TGTCCCTTGTTCGAAGGGAT+TGG 0.425173 8_3:+15135597 Msa1278670:CDS
AGTGAAAGTAGATGAGGATT+CGG 0.428936 8_3:+15134590 None:intergenic
TACTAGTAGTGATAATGTTA+AGG 0.430645 8_3:+15135528 Msa1278670:CDS
TTTAGGGACCTTTCTTTCAC+TGG 0.431289 8_3:-15135688 None:intergenic
ATATAGTGTGGATTCGTTGA+TGG 0.437744 8_3:+15135189 Msa1278670:intron
GAAGAAGTCATTGGTGGGTT+CGG 0.439883 8_3:+15135381 Msa1278670:CDS
CAAGGAAGCCAGTGAAAGAA+AGG 0.466608 8_3:+15135680 Msa1278670:CDS
GGATTATGTTGAAGATTATG+AGG 0.475571 8_3:+15135618 Msa1278670:CDS
GATAATGTTAAGGTTAATGT+TGG 0.478265 8_3:+15135538 Msa1278670:CDS
GTGAATTGAGGAGGAGTTGT+TGG 0.479999 8_3:-15134749 None:intergenic
ATGATTCTGGTGATGATAAT+GGG 0.482433 8_3:+15135650 Msa1278670:CDS
GGATCACATCCCATGGAGAT+TGG 0.490080 8_3:-15134717 None:intergenic
AAGGGATATTTATATAGATA+GGG 0.497218 8_3:+15135725 Msa1278670:three_prime_UTR
GTTTGGAACCTGGTGAATTG+AGG 0.498578 8_3:-15134761 None:intergenic
GATATTTATATAGATAGGGT+TGG 0.505707 8_3:+15135729 Msa1278670:three_prime_UTR
ATGAACAAGTGAATAGAAGA+GGG 0.507830 8_3:-15134630 None:intergenic
ATGATAATGGGAGGAGAACA+AGG 0.509142 8_3:+15135662 Msa1278670:CDS
GTCAACTTAACCAATCTCCA+TGG 0.512228 8_3:+15134707 Msa1278670:CDS
TGCGATTGAAAATTATGAAA+TGG 0.512443 8_3:+15135246 Msa1278670:CDS
AATGTTGTCCCTTGTTCGAA+GGG 0.513132 8_3:+15135592 Msa1278670:CDS
CCTTTCATCAAGAGCTCCAA+AGG 0.518678 8_3:-15135008 None:intergenic
CCTTTGGAGCTCTTGATGAA+AGG 0.521590 8_3:+15135008 Msa1278670:CDS
CTAAAATCGTTAATGTGAGA+AGG 0.524077 8_3:+15135706 Msa1278670:exon
TGTATTGTTTGTTCTTATGG+TGG 0.524557 8_3:+15135802 Msa1278670:three_prime_UTR
TTTCGTTGTTTCCGCCGCGA+TGG 0.527941 8_3:-15135451 None:intergenic
AATAATGGTGGCAGCAACAA+AGG 0.529728 8_3:+15135295 Msa1278670:CDS
AGGCCGACCGAAGAAGTCAT+TGG 0.537961 8_3:+15135372 Msa1278670:CDS
GATGAACAAGTGAATAGAAG+AGG 0.539010 8_3:-15134631 None:intergenic
GAACCCACCAATGACTTCTT+CGG 0.541039 8_3:-15135379 None:intergenic
GAAGAAAGTGAAAGTAGATG+AGG 0.542687 8_3:+15134584 None:intergenic
AATTGAGGAGGAGTTGTTGG+AGG 0.546737 8_3:-15134746 None:intergenic
TAATGTTGTCCCTTGTTCGA+AGG 0.547501 8_3:+15135591 Msa1278670:CDS
TTGGTTAAGTTGACAAACAT+GGG 0.552844 8_3:-15134698 None:intergenic
TAAAATCGTTAATGTGAGAA+GGG 0.557477 8_3:+15135707 Msa1278670:exon
CAACTCCTCCTCAATTCACC+AGG 0.560031 8_3:+15134753 Msa1278670:CDS
ATTGGTTAAGTTGACAAACA+TGG 0.560560 8_3:-15134699 None:intergenic
TAATCCAATCCCTTCGAACA+AGG 0.560699 8_3:-15135601 None:intergenic
TCAACTTAACCAATCTCCAT+GGG 0.567539 8_3:+15134708 Msa1278670:CDS
TGAAGATAACAAGAGTGTTG+TGG 0.571409 8_3:+15135495 Msa1278670:CDS
TATTTATATAGATAGGGTTG+GGG 0.571755 8_3:+15135731 Msa1278670:three_prime_UTR
GAGGCGCCGCGACGTTGTAG+AGG 0.576078 8_3:+15135352 Msa1278670:CDS
GTTTGAAGAGAAACTCGAAT+GGG 0.587709 8_3:+15135317 Msa1278670:CDS
GCAAAGGGGATCACATCCCA+TGG 0.588226 8_3:-15134724 None:intergenic
TCGCGGCGGAAACAACGAAA+GGG 0.598136 8_3:+15135454 Msa1278670:CDS
TGGAACCTGGTGAATTGAGG+AGG 0.607964 8_3:-15134758 None:intergenic
ATCGCGGCGGAAACAACGAA+AGG 0.612725 8_3:+15135453 Msa1278670:CDS
AAGGTGGTTGATAATAATGG+TGG 0.617164 8_3:+15135283 Msa1278670:CDS
TGAACAAGTGAATAGAAGAG+GGG 0.618395 8_3:-15134629 None:intergenic
CGACCGAAGAAGTCATTGGT+GGG 0.625633 8_3:+15135376 Msa1278670:CDS
CCGACCGAAGAAGTCATTGG+TGG 0.626091 8_3:+15135375 Msa1278670:CDS
GGTGTTTGATGATAATAAGG+TGG 0.630851 8_3:+15135267 Msa1278670:CDS
AATCCAATCCCTTCGAACAA+GGG 0.636829 8_3:-15135600 None:intergenic
AAGGGTGATAGTAAAAGTGA+AGG 0.640778 8_3:+15135472 Msa1278670:CDS
TTTGAAGAGAAACTCGAATG+GGG 0.644320 8_3:+15135318 Msa1278670:CDS
GGTTTGAAGAGAAACTCGAA+TGG 0.644835 8_3:+15135316 Msa1278670:CDS
CTGGGTTTGGTCCATCGCGG+CGG 0.654002 8_3:+15135440 Msa1278670:CDS
AGGAGTTGTTGGAGGCAAAG+GGG 0.661260 8_3:-15134738 None:intergenic
CCACCAATGACTTCTTCGGT+CGG 0.687410 8_3:-15135375 None:intergenic
TTGAAGAGAAACTCGAATGG+GGG 0.687747 8_3:+15135319 Msa1278670:CDS
ATTCTGGGTTTGGTCCATCG+CGG 0.711134 8_3:+15135437 Msa1278670:CDS
GGTCGGCCTCTACAACGTCG+CGG 0.724123 8_3:-15135358 None:intergenic
ATTCTGGTGATGATAATGGG+AGG 0.724688 8_3:+15135653 Msa1278670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTATTGTTAATTTTTTTT+TGG + chr8_3:15135766-15135785 Msa1278670:three_prime_UTR 10.0%
!!! TTGTATTGTTAATTTTTTTT+TGG + chr8_3:15135766-15135785 Msa1278670:three_prime_UTR 10.0%
!!! TTTTTTCATATGCTTTATTA+GGG + chr8_3:15134813-15134832 Msa1278670:intron 15.0%
!! AATGAATTCTATTATTATTC+TGG + chr8_3:15135421-15135440 Msa1278670:CDS 15.0%
!! ATGAATTCTATTATTATTCT+GGG + chr8_3:15135422-15135441 Msa1278670:CDS 15.0%
!!! TTTTTTCATATGCTTTATTA+GGG + chr8_3:15134813-15134832 Msa1278670:intron 15.0%
!! AATGAATTCTATTATTATTC+TGG + chr8_3:15135421-15135440 Msa1278670:CDS 15.0%
!! ATGAATTCTATTATTATTCT+GGG + chr8_3:15135422-15135441 Msa1278670:CDS 15.0%
!!! GTTTTTTCATATGCTTTATT+AGG + chr8_3:15134812-15134831 Msa1278670:intron 20.0%
!! AGTAAAAAAGATAACGAATT+GGG - chr8_3:15135051-15135070 None:intergenic 20.0%
!! AAGTAAAAAAGATAACGAAT+TGG - chr8_3:15135052-15135071 None:intergenic 20.0%
!!! TGTTTGAATGAATTTTTGTT+TGG + chr8_3:15135149-15135168 Msa1278670:intron 20.0%
!! AATGATGAAAAAATATAGTG+TGG + chr8_3:15135177-15135196 Msa1278670:intron 20.0%
!! AAGGGATATTTATATAGATA+GGG + chr8_3:15135725-15135744 Msa1278670:three_prime_UTR 20.0%
!! ATATTTATATAGATAGGGTT+GGG + chr8_3:15135730-15135749 Msa1278670:three_prime_UTR 20.0%
!!! GTTTTTTCATATGCTTTATT+AGG + chr8_3:15134812-15134831 Msa1278670:intron 20.0%
!! AGTAAAAAAGATAACGAATT+GGG - chr8_3:15135051-15135070 None:intergenic 20.0%
!! AAGTAAAAAAGATAACGAAT+TGG - chr8_3:15135052-15135071 None:intergenic 20.0%
!!! TGTTTGAATGAATTTTTGTT+TGG + chr8_3:15135149-15135168 Msa1278670:intron 20.0%
!! AATGATGAAAAAATATAGTG+TGG + chr8_3:15135177-15135196 Msa1278670:intron 20.0%
!! AAGGGATATTTATATAGATA+GGG + chr8_3:15135725-15135744 Msa1278670:three_prime_UTR 20.0%
!! ATATTTATATAGATAGGGTT+GGG + chr8_3:15135730-15135749 Msa1278670:three_prime_UTR 20.0%
! TTCTCTTGTCATATCTATTA+GGG + chr8_3:15134790-15134809 Msa1278670:intron 25.0%
!!! AACCTATTTTGAAGCAAAAA+GGG - chr8_3:15134892-15134911 None:intergenic 25.0%
!!! TAACCTATTTTGAAGCAAAA+AGG - chr8_3:15134893-15134912 None:intergenic 25.0%
! TTTGCTTCAAAATAGGTTAA+TGG + chr8_3:15134894-15134913 Msa1278670:intron 25.0%
! TTGCTTCAAAATAGGTTAAT+GGG + chr8_3:15134895-15134914 Msa1278670:intron 25.0%
!! AGCAAGATATTTTCACAAAT+GGG + chr8_3:15134981-15135000 Msa1278670:CDS 25.0%
! GTAGAAAAAAAAACCCTAAT+GGG - chr8_3:15135086-15135105 None:intergenic 25.0%
! AGTAGAAAAAAAAACCCTAA+TGG - chr8_3:15135087-15135106 None:intergenic 25.0%
!! ATTTCATAATTTTCAATCGC+AGG - chr8_3:15135247-15135266 None:intergenic 25.0%
! TGCGATTGAAAATTATGAAA+TGG + chr8_3:15135246-15135265 Msa1278670:CDS 25.0%
!! AATGGTGTTTGATGATAATA+AGG + chr8_3:15135264-15135283 Msa1278670:CDS 25.0%
!!! AATAAGGTGGTTGATAATAA+TGG + chr8_3:15135280-15135299 Msa1278670:CDS 25.0%
! TTCTATTATTATTCTGGGTT+TGG + chr8_3:15135427-15135446 Msa1278670:CDS 25.0%
!! TACTAGTAGTGATAATGTTA+AGG + chr8_3:15135528-15135547 Msa1278670:CDS 25.0%
! GATAATGTTAAGGTTAATGT+TGG + chr8_3:15135538-15135557 Msa1278670:CDS 25.0%
!! TTCTCACATTAACGATTTTA+GGG - chr8_3:15135707-15135726 None:intergenic 25.0%
! TAAAATCGTTAATGTGAGAA+GGG + chr8_3:15135707-15135726 Msa1278670:exon 25.0%
! GAAGGGATATTTATATAGAT+AGG + chr8_3:15135724-15135743 Msa1278670:three_prime_UTR 25.0%
! GATATTTATATAGATAGGGT+TGG + chr8_3:15135729-15135748 Msa1278670:three_prime_UTR 25.0%
! TATTTATATAGATAGGGTTG+GGG + chr8_3:15135731-15135750 Msa1278670:three_prime_UTR 25.0%
! TGTTGTATTGTTTGTTCTTA+TGG + chr8_3:15135799-15135818 Msa1278670:three_prime_UTR 25.0%
! TTCTCTTGTCATATCTATTA+GGG + chr8_3:15134790-15134809 Msa1278670:intron 25.0%
!!! AACCTATTTTGAAGCAAAAA+GGG - chr8_3:15134892-15134911 None:intergenic 25.0%
!!! TAACCTATTTTGAAGCAAAA+AGG - chr8_3:15134893-15134912 None:intergenic 25.0%
! TTTGCTTCAAAATAGGTTAA+TGG + chr8_3:15134894-15134913 Msa1278670:intron 25.0%
! TTGCTTCAAAATAGGTTAAT+GGG + chr8_3:15134895-15134914 Msa1278670:intron 25.0%
!! AGCAAGATATTTTCACAAAT+GGG + chr8_3:15134981-15135000 Msa1278670:CDS 25.0%
! GTAGAAAAAAAAACCCTAAT+GGG - chr8_3:15135086-15135105 None:intergenic 25.0%
! AGTAGAAAAAAAAACCCTAA+TGG - chr8_3:15135087-15135106 None:intergenic 25.0%
!! ATTTCATAATTTTCAATCGC+AGG - chr8_3:15135247-15135266 None:intergenic 25.0%
! TGCGATTGAAAATTATGAAA+TGG + chr8_3:15135246-15135265 Msa1278670:CDS 25.0%
!! AATGGTGTTTGATGATAATA+AGG + chr8_3:15135264-15135283 Msa1278670:CDS 25.0%
!!! AATAAGGTGGTTGATAATAA+TGG + chr8_3:15135280-15135299 Msa1278670:CDS 25.0%
! TTCTATTATTATTCTGGGTT+TGG + chr8_3:15135427-15135446 Msa1278670:CDS 25.0%
!! TACTAGTAGTGATAATGTTA+AGG + chr8_3:15135528-15135547 Msa1278670:CDS 25.0%
! GATAATGTTAAGGTTAATGT+TGG + chr8_3:15135538-15135557 Msa1278670:CDS 25.0%
!! TTCTCACATTAACGATTTTA+GGG - chr8_3:15135707-15135726 None:intergenic 25.0%
! TAAAATCGTTAATGTGAGAA+GGG + chr8_3:15135707-15135726 Msa1278670:exon 25.0%
! GAAGGGATATTTATATAGAT+AGG + chr8_3:15135724-15135743 Msa1278670:three_prime_UTR 25.0%
! GATATTTATATAGATAGGGT+TGG + chr8_3:15135729-15135748 Msa1278670:three_prime_UTR 25.0%
! TATTTATATAGATAGGGTTG+GGG + chr8_3:15135731-15135750 Msa1278670:three_prime_UTR 25.0%
! TGTTGTATTGTTTGTTCTTA+TGG + chr8_3:15135799-15135818 Msa1278670:three_prime_UTR 25.0%
ATGAACAAGTGAATAGAAGA+GGG - chr8_3:15134633-15134652 None:intergenic 30.0%
TCATCTTCACTTCTACAAAA+TGG + chr8_3:15134649-15134668 Msa1278670:CDS 30.0%
TGGGTTTGAAAAATTTCAAC+TGG - chr8_3:15134682-15134701 None:intergenic 30.0%
!! TTGGTTAAGTTGACAAACAT+GGG - chr8_3:15134701-15134720 None:intergenic 30.0%
!! ATTGGTTAAGTTGACAAACA+TGG - chr8_3:15134702-15134721 None:intergenic 30.0%
CTTCTCTTGTCATATCTATT+AGG + chr8_3:15134789-15134808 Msa1278670:intron 30.0%
TTGTGAAAATATCTTGCTCT+TGG - chr8_3:15134979-15134998 None:intergenic 30.0%
! GAGCAAGATATTTTCACAAA+TGG + chr8_3:15134980-15134999 Msa1278670:CDS 30.0%
! GCAAGATATTTTCACAAATG+GGG + chr8_3:15134982-15135001 Msa1278670:CDS 30.0%
!! TTAACTTGCAAAACCCATTA+GGG + chr8_3:15135070-15135089 Msa1278670:intron 30.0%
GGATTATGTTGAAGATTATG+AGG + chr8_3:15135618-15135637 Msa1278670:CDS 30.0%
! ATGATTCTGGTGATGATAAT+GGG + chr8_3:15135650-15135669 Msa1278670:CDS 30.0%
! CTTCTCACATTAACGATTTT+AGG - chr8_3:15135708-15135727 None:intergenic 30.0%
CTAAAATCGTTAATGTGAGA+AGG + chr8_3:15135706-15135725 Msa1278670:exon 30.0%
TGTATTGTTTGTTCTTATGG+TGG + chr8_3:15135802-15135821 Msa1278670:three_prime_UTR 30.0%
ATGAACAAGTGAATAGAAGA+GGG - chr8_3:15134633-15134652 None:intergenic 30.0%
TCATCTTCACTTCTACAAAA+TGG + chr8_3:15134649-15134668 Msa1278670:CDS 30.0%
TGGGTTTGAAAAATTTCAAC+TGG - chr8_3:15134682-15134701 None:intergenic 30.0%
!! TTGGTTAAGTTGACAAACAT+GGG - chr8_3:15134701-15134720 None:intergenic 30.0%
!! ATTGGTTAAGTTGACAAACA+TGG - chr8_3:15134702-15134721 None:intergenic 30.0%
CTTCTCTTGTCATATCTATT+AGG + chr8_3:15134789-15134808 Msa1278670:intron 30.0%
TTGTGAAAATATCTTGCTCT+TGG - chr8_3:15134979-15134998 None:intergenic 30.0%
! GAGCAAGATATTTTCACAAA+TGG + chr8_3:15134980-15134999 Msa1278670:CDS 30.0%
! GCAAGATATTTTCACAAATG+GGG + chr8_3:15134982-15135001 Msa1278670:CDS 30.0%
!! TTAACTTGCAAAACCCATTA+GGG + chr8_3:15135070-15135089 Msa1278670:intron 30.0%
GGATTATGTTGAAGATTATG+AGG + chr8_3:15135618-15135637 Msa1278670:CDS 30.0%
! ATGATTCTGGTGATGATAAT+GGG + chr8_3:15135650-15135669 Msa1278670:CDS 30.0%
! CTTCTCACATTAACGATTTT+AGG - chr8_3:15135708-15135727 None:intergenic 30.0%
CTAAAATCGTTAATGTGAGA+AGG + chr8_3:15135706-15135725 Msa1278670:exon 30.0%
TGTATTGTTTGTTCTTATGG+TGG + chr8_3:15135802-15135821 Msa1278670:three_prime_UTR 30.0%
TGAACAAGTGAATAGAAGAG+GGG - chr8_3:15134632-15134651 None:intergenic 35.0%
GATGAACAAGTGAATAGAAG+AGG - chr8_3:15134634-15134653 None:intergenic 35.0%
TCAACTTAACCAATCTCCAT+GGG + chr8_3:15134708-15134727 Msa1278670:CDS 35.0%
TGACAAGAGAAGAAGAAGTT+TGG - chr8_3:15134781-15134800 None:intergenic 35.0%
! TGCCCTTTTTGCTTCAAAAT+AGG + chr8_3:15134887-15134906 Msa1278670:intron 35.0%
!! CTTAACTTGCAAAACCCATT+AGG + chr8_3:15135069-15135088 Msa1278670:intron 35.0%
ATATAGTGTGGATTCGTTGA+TGG + chr8_3:15135189-15135208 Msa1278670:intron 35.0%
! GGTGTTTGATGATAATAAGG+TGG + chr8_3:15135267-15135286 Msa1278670:CDS 35.0%
!! AAGGTGGTTGATAATAATGG+TGG + chr8_3:15135283-15135302 Msa1278670:CDS 35.0%
GTTTGAAGAGAAACTCGAAT+GGG + chr8_3:15135317-15135336 Msa1278670:CDS 35.0%
TTTGAAGAGAAACTCGAATG+GGG + chr8_3:15135318-15135337 Msa1278670:CDS 35.0%
AAGGGTGATAGTAAAAGTGA+AGG + chr8_3:15135472-15135491 Msa1278670:CDS 35.0%
!! TGAAGATAACAAGAGTGTTG+TGG + chr8_3:15135495-15135514 Msa1278670:CDS 35.0%
!! AACAAGAGTGTTGTGGAATT+AGG + chr8_3:15135502-15135521 Msa1278670:CDS 35.0%
GAGGATGATTATGATGATTC+TGG + chr8_3:15135637-15135656 Msa1278670:CDS 35.0%
! GATGATTCTGGTGATGATAA+TGG + chr8_3:15135649-15135668 Msa1278670:CDS 35.0%
TGAACAAGTGAATAGAAGAG+GGG - chr8_3:15134632-15134651 None:intergenic 35.0%
GATGAACAAGTGAATAGAAG+AGG - chr8_3:15134634-15134653 None:intergenic 35.0%
TCAACTTAACCAATCTCCAT+GGG + chr8_3:15134708-15134727 Msa1278670:CDS 35.0%
TGACAAGAGAAGAAGAAGTT+TGG - chr8_3:15134781-15134800 None:intergenic 35.0%
! TGCCCTTTTTGCTTCAAAAT+AGG + chr8_3:15134887-15134906 Msa1278670:intron 35.0%
!! CTTAACTTGCAAAACCCATT+AGG + chr8_3:15135069-15135088 Msa1278670:intron 35.0%
ATATAGTGTGGATTCGTTGA+TGG + chr8_3:15135189-15135208 Msa1278670:intron 35.0%
! GGTGTTTGATGATAATAAGG+TGG + chr8_3:15135267-15135286 Msa1278670:CDS 35.0%
!! AAGGTGGTTGATAATAATGG+TGG + chr8_3:15135283-15135302 Msa1278670:CDS 35.0%
GTTTGAAGAGAAACTCGAAT+GGG + chr8_3:15135317-15135336 Msa1278670:CDS 35.0%
TTTGAAGAGAAACTCGAATG+GGG + chr8_3:15135318-15135337 Msa1278670:CDS 35.0%
AAGGGTGATAGTAAAAGTGA+AGG + chr8_3:15135472-15135491 Msa1278670:CDS 35.0%
!! TGAAGATAACAAGAGTGTTG+TGG + chr8_3:15135495-15135514 Msa1278670:CDS 35.0%
!! AACAAGAGTGTTGTGGAATT+AGG + chr8_3:15135502-15135521 Msa1278670:CDS 35.0%
GAGGATGATTATGATGATTC+TGG + chr8_3:15135637-15135656 Msa1278670:CDS 35.0%
! GATGATTCTGGTGATGATAA+TGG + chr8_3:15135649-15135668 Msa1278670:CDS 35.0%
GTCAACTTAACCAATCTCCA+TGG + chr8_3:15134707-15134726 Msa1278670:CDS 40.0%
! AGAAGAAGAAGTTTGGAACC+TGG - chr8_3:15134774-15134793 None:intergenic 40.0%
TTCACAAATGGGGATTCCTT+TGG + chr8_3:15134992-15135011 Msa1278670:CDS 40.0%
AATAATGGTGGCAGCAACAA+AGG + chr8_3:15135295-15135314 Msa1278670:CDS 40.0%
GGTTTGAAGAGAAACTCGAA+TGG + chr8_3:15135316-15135335 Msa1278670:CDS 40.0%
TTGAAGAGAAACTCGAATGG+GGG + chr8_3:15135319-15135338 Msa1278670:CDS 40.0%
TAATGTTGTCCCTTGTTCGA+AGG + chr8_3:15135591-15135610 Msa1278670:CDS 40.0%
AATGTTGTCCCTTGTTCGAA+GGG + chr8_3:15135592-15135611 Msa1278670:CDS 40.0%
AATCCAATCCCTTCGAACAA+GGG - chr8_3:15135603-15135622 None:intergenic 40.0%
TAATCCAATCCCTTCGAACA+AGG - chr8_3:15135604-15135623 None:intergenic 40.0%
!! ATTCTGGTGATGATAATGGG+AGG + chr8_3:15135653-15135672 Msa1278670:CDS 40.0%
!! ATGATAATGGGAGGAGAACA+AGG + chr8_3:15135662-15135681 Msa1278670:CDS 40.0%
TTTAGGGACCTTTCTTTCAC+TGG - chr8_3:15135691-15135710 None:intergenic 40.0%
GTCAACTTAACCAATCTCCA+TGG + chr8_3:15134707-15134726 Msa1278670:CDS 40.0%
! AGAAGAAGAAGTTTGGAACC+TGG - chr8_3:15134774-15134793 None:intergenic 40.0%
TTCACAAATGGGGATTCCTT+TGG + chr8_3:15134992-15135011 Msa1278670:CDS 40.0%
AATAATGGTGGCAGCAACAA+AGG + chr8_3:15135295-15135314 Msa1278670:CDS 40.0%
GGTTTGAAGAGAAACTCGAA+TGG + chr8_3:15135316-15135335 Msa1278670:CDS 40.0%
TTGAAGAGAAACTCGAATGG+GGG + chr8_3:15135319-15135338 Msa1278670:CDS 40.0%
TAATGTTGTCCCTTGTTCGA+AGG + chr8_3:15135591-15135610 Msa1278670:CDS 40.0%
AATGTTGTCCCTTGTTCGAA+GGG + chr8_3:15135592-15135611 Msa1278670:CDS 40.0%
AATCCAATCCCTTCGAACAA+GGG - chr8_3:15135603-15135622 None:intergenic 40.0%
TAATCCAATCCCTTCGAACA+AGG - chr8_3:15135604-15135623 None:intergenic 40.0%
!! ATTCTGGTGATGATAATGGG+AGG + chr8_3:15135653-15135672 Msa1278670:CDS 40.0%
!! ATGATAATGGGAGGAGAACA+AGG + chr8_3:15135662-15135681 Msa1278670:CDS 40.0%
TTTAGGGACCTTTCTTTCAC+TGG - chr8_3:15135691-15135710 None:intergenic 40.0%
AATTGAGGAGGAGTTGTTGG+AGG - chr8_3:15134749-15134768 None:intergenic 45.0%
GTGAATTGAGGAGGAGTTGT+TGG - chr8_3:15134752-15134771 None:intergenic 45.0%
! GTTTGGAACCTGGTGAATTG+AGG - chr8_3:15134764-15134783 None:intergenic 45.0%
CCTTTCATCAAGAGCTCCAA+AGG - chr8_3:15135011-15135030 None:intergenic 45.0%
!! CCTTTGGAGCTCTTGATGAA+AGG + chr8_3:15135008-15135027 Msa1278670:CDS 45.0%
GAACCCACCAATGACTTCTT+CGG - chr8_3:15135382-15135401 None:intergenic 45.0%
!! GAAGAAGTCATTGGTGGGTT+CGG + chr8_3:15135381-15135400 Msa1278670:CDS 45.0%
CAAGGAAGCCAGTGAAAGAA+AGG + chr8_3:15135680-15135699 Msa1278670:CDS 45.0%
AATTGAGGAGGAGTTGTTGG+AGG - chr8_3:15134749-15134768 None:intergenic 45.0%
GTGAATTGAGGAGGAGTTGT+TGG - chr8_3:15134752-15134771 None:intergenic 45.0%
! GTTTGGAACCTGGTGAATTG+AGG - chr8_3:15134764-15134783 None:intergenic 45.0%
CCTTTCATCAAGAGCTCCAA+AGG - chr8_3:15135011-15135030 None:intergenic 45.0%
!! CCTTTGGAGCTCTTGATGAA+AGG + chr8_3:15135008-15135027 Msa1278670:CDS 45.0%
GAACCCACCAATGACTTCTT+CGG - chr8_3:15135382-15135401 None:intergenic 45.0%
!! GAAGAAGTCATTGGTGGGTT+CGG + chr8_3:15135381-15135400 Msa1278670:CDS 45.0%
CAAGGAAGCCAGTGAAAGAA+AGG + chr8_3:15135680-15135699 Msa1278670:CDS 45.0%
GGATCACATCCCATGGAGAT+TGG - chr8_3:15134720-15134739 None:intergenic 50.0%
AGGAGTTGTTGGAGGCAAAG+GGG - chr8_3:15134741-15134760 None:intergenic 50.0%
GAGGAGTTGTTGGAGGCAAA+GGG - chr8_3:15134742-15134761 None:intergenic 50.0%
CAACTCCTCCTCAATTCACC+AGG + chr8_3:15134753-15134772 Msa1278670:CDS 50.0%
TGGAACCTGGTGAATTGAGG+AGG - chr8_3:15134761-15134780 None:intergenic 50.0%
AAGAGAAACTCGAATGGGGG+TGG + chr8_3:15135322-15135341 Msa1278670:CDS 50.0%
CCACCAATGACTTCTTCGGT+CGG - chr8_3:15135378-15135397 None:intergenic 50.0%
CGACCGAAGAAGTCATTGGT+GGG + chr8_3:15135376-15135395 Msa1278670:CDS 50.0%
! ATTCTGGGTTTGGTCCATCG+CGG + chr8_3:15135437-15135456 Msa1278670:CDS 50.0%
TGTCCCTTGTTCGAAGGGAT+TGG + chr8_3:15135597-15135616 Msa1278670:CDS 50.0%
GGATCACATCCCATGGAGAT+TGG - chr8_3:15134720-15134739 None:intergenic 50.0%
AGGAGTTGTTGGAGGCAAAG+GGG - chr8_3:15134741-15134760 None:intergenic 50.0%
GAGGAGTTGTTGGAGGCAAA+GGG - chr8_3:15134742-15134761 None:intergenic 50.0%
CAACTCCTCCTCAATTCACC+AGG + chr8_3:15134753-15134772 Msa1278670:CDS 50.0%
TGGAACCTGGTGAATTGAGG+AGG - chr8_3:15134761-15134780 None:intergenic 50.0%
AAGAGAAACTCGAATGGGGG+TGG + chr8_3:15135322-15135341 Msa1278670:CDS 50.0%
CCACCAATGACTTCTTCGGT+CGG - chr8_3:15135378-15135397 None:intergenic 50.0%
CGACCGAAGAAGTCATTGGT+GGG + chr8_3:15135376-15135395 Msa1278670:CDS 50.0%
! ATTCTGGGTTTGGTCCATCG+CGG + chr8_3:15135437-15135456 Msa1278670:CDS 50.0%
TGTCCCTTGTTCGAAGGGAT+TGG + chr8_3:15135597-15135616 Msa1278670:CDS 50.0%
GCAAAGGGGATCACATCCCA+TGG - chr8_3:15134727-15134746 None:intergenic 55.0%
GGAGGAGTTGTTGGAGGCAA+AGG - chr8_3:15134743-15134762 None:intergenic 55.0%
AGGCCGACCGAAGAAGTCAT+TGG + chr8_3:15135372-15135391 Msa1278670:CDS 55.0%
CCGACCGAAGAAGTCATTGG+TGG + chr8_3:15135375-15135394 Msa1278670:CDS 55.0%
!! GTCATTGGTGGGTTCGGCTT+CGG + chr8_3:15135387-15135406 Msa1278670:CDS 55.0%
!! TCATTGGTGGGTTCGGCTTC+GGG + chr8_3:15135388-15135407 Msa1278670:CDS 55.0%
! TTTCGTTGTTTCCGCCGCGA+TGG - chr8_3:15135454-15135473 None:intergenic 55.0%
ATCGCGGCGGAAACAACGAA+AGG + chr8_3:15135453-15135472 Msa1278670:CDS 55.0%
TCGCGGCGGAAACAACGAAA+GGG + chr8_3:15135454-15135473 Msa1278670:CDS 55.0%
GCAAAGGGGATCACATCCCA+TGG - chr8_3:15134727-15134746 None:intergenic 55.0%
GGAGGAGTTGTTGGAGGCAA+AGG - chr8_3:15134743-15134762 None:intergenic 55.0%
AGGCCGACCGAAGAAGTCAT+TGG + chr8_3:15135372-15135391 Msa1278670:CDS 55.0%
CCGACCGAAGAAGTCATTGG+TGG + chr8_3:15135375-15135394 Msa1278670:CDS 55.0%
!! GTCATTGGTGGGTTCGGCTT+CGG + chr8_3:15135387-15135406 Msa1278670:CDS 55.0%
!! TCATTGGTGGGTTCGGCTTC+GGG + chr8_3:15135388-15135407 Msa1278670:CDS 55.0%
! TTTCGTTGTTTCCGCCGCGA+TGG - chr8_3:15135454-15135473 None:intergenic 55.0%
ATCGCGGCGGAAACAACGAA+AGG + chr8_3:15135453-15135472 Msa1278670:CDS 55.0%
TCGCGGCGGAAACAACGAAA+GGG + chr8_3:15135454-15135473 Msa1278670:CDS 55.0%
GAATGGGGGTGGCAGCATTG+AGG + chr8_3:15135333-15135352 Msa1278670:CDS 60.0%
GAATGGGGGTGGCAGCATTG+AGG + chr8_3:15135333-15135352 Msa1278670:CDS 60.0%
GGTCGGCCTCTACAACGTCG+CGG - chr8_3:15135361-15135380 None:intergenic 65.0%
! CTGGGTTTGGTCCATCGCGG+CGG + chr8_3:15135440-15135459 Msa1278670:CDS 65.0%
GGTCGGCCTCTACAACGTCG+CGG - chr8_3:15135361-15135380 None:intergenic 65.0%
! CTGGGTTTGGTCCATCGCGG+CGG + chr8_3:15135440-15135459 Msa1278670:CDS 65.0%
!! GAGGCGCCGCGACGTTGTAG+AGG + chr8_3:15135352-15135371 Msa1278670:CDS 70.0%
!! GAGGCGCCGCGACGTTGTAG+AGG + chr8_3:15135352-15135371 Msa1278670:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 15134591 15135834 15134591 ID=Msa1278670;Name=Msa1278670
chr8_3 mRNA 15134591 15135834 15134591 ID=Msa1278670-mRNA-1;Parent=Msa1278670;Name=Msa1278670-mRNA-1;_AED=0.07;_eAED=0.07;_QI=10|1|1|1|1|1|3|111|252
chr8_3 exon 15134591 15134774 15134591 ID=Msa1278670-mRNA-1:exon:5864;Parent=Msa1278670-mRNA-1
chr8_3 exon 15134974 15135029 15134974 ID=Msa1278670-mRNA-1:exon:5865;Parent=Msa1278670-mRNA-1
chr8_3 exon 15135195 15135834 15135195 ID=Msa1278670-mRNA-1:exon:5866;Parent=Msa1278670-mRNA-1
chr8_3 five_prime_UTR 15134591 15134600 15134591 ID=Msa1278670-mRNA-1:five_prime_utr;Parent=Msa1278670-mRNA-1
chr8_3 CDS 15134601 15134774 15134601 ID=Msa1278670-mRNA-1:cds;Parent=Msa1278670-mRNA-1
chr8_3 CDS 15134974 15135029 15134974 ID=Msa1278670-mRNA-1:cds;Parent=Msa1278670-mRNA-1
chr8_3 CDS 15135195 15135723 15135195 ID=Msa1278670-mRNA-1:cds;Parent=Msa1278670-mRNA-1
chr8_3 three_prime_UTR 15135724 15135834 15135724 ID=Msa1278670-mRNA-1:three_prime_utr;Parent=Msa1278670-mRNA-1
Gene Sequence

>Msa1278670

ATGAGGATTCGGAAAAACACAGAGCTTTCCCCTCTTCTATTCACTTGTTCATCTTCACTTCTACAAAATGGTTCATTTCCAGTTGAAATTTTTCAAACCCATGTTTGTCAACTTAACCAATCTCCATGGGATGTGATCCCCTTTGCCTCCAACAACTCCTCCTCAATTCACCAGTTCCAAGAGCAAGATATTTTCACAAATGGGGATTCCTTTGGAGCTCTTGATGAAAGTGTGGATTCGTTGATGGAAACTGAAGTTGTTGATGCTGATAACAACAAACCTGCGATTGAAAATTATGAAATGGTGTTTGATGATAATAAGGTGGTTGATAATAATGGTGGCAGCAACAAAGGTTTGAAGAGAAACTCGAATGGGGGTGGCAGCATTGAGGCGCCGCGACGTTGTAGAGGCCGACCGAAGAAGTCATTGGTGGGTTCGGCTTCGGGTTCAAATAATAATGAATTCTATTATTATTCTGGGTTTGGTCCATCGCGGCGGAAACAACGAAAGGGTGATAGTAAAAGTGAAGGTGAAGATAACAAGAGTGTTGTGGAATTAGGTTCTACTAGTAGTGATAATGTTAAGGTTAATGTTGGTAATAATAATGTTGTTGCTAGTGTTGATGCTAATGTTGTCCCTTGTTCGAAGGGATTGGATTATGTTGAAGATTATGAGGATGATTATGATGATTCTGGTGATGATAATGGGAGGAGAACAAGGAAGCCAGTGAAAGAAAGGTCCCTAAAATCGTTAATGTGA
Protein sequence

>Msa1278670

MRIRKNTELSPLLFTCSSSLLQNGSFPVEIFQTHVCQLNQSPWDVIPFASNNSSSIHQFQEQDIFTNGDSFGALDESVDSLMETEVVDADNNKPAIENYEMVFDDNKVVDNNGGSNKGLKRNSNGGGSIEAPRRCRGRPKKSLVGSASGSNNNEFYYYSGFGPSRRKQRKGDSKSEGEDNKSVVELGSTSSDNVKVNVGNNNVVASVDANVVPCSKGLDYVEDYEDDYDDSGDDNGRRTRKPVKERSLKSLM*