AlfalfaGEDB | Msa1278670

M. sativa cultivar zhongmu-4 / Msa1278670

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1278670	A0A396GMD0	86.275	255	31	4	1	252	1	254	2.88e-138	398

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1278670	Msa1368080	0.811632	6.352455e-51	-8.615850e-47
Msa1278670	Msa1373170	0.801291	9.649256e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1278670	MtrunA17_Chr8g0348581	86.275	255	31	4	1	252	1	254	5.54e-142	398
Msa1278670	MtrunA17_Chr8g0348571	91.333	150	10	3	1	149	1	148	4.83e-92	270
Msa1278670	MtrunA17_Chr3g0083791	62.346	162	42	4	1	154	1	151	5.91e-58	181
Msa1278670	MtrunA17_Chr8g0348601	54.626	227	65	12	20	238	3	199	5.80e-57	180
Msa1278670	MtrunA17_Chr3g0083161	61.728	162	43	4	1	154	1	151	7.30e-57	178

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1278670	AT4G00310.2	30.284	317	141	14	1	252	1	302	1.55e-16	77.8
Msa1278670	AT4G00310.1	30.284	317	141	14	1	252	1	302	1.55e-16	77.8

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Find 80 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGAGTGTTGTGGAATT+AGG	0.304116	8_3:+15135502	Msa1278670:CDS
AAGGGATATTTATATAGATA+GGG	0.497218	8_3:+15135725	Msa1278670:three_prime_UTR
AAGGGTGATAGTAAAAGTGA+AGG	0.640778	8_3:+15135472	Msa1278670:CDS
AAGGTGGTTGATAATAATGG+TGG	0.617164	8_3:+15135283	Msa1278670:CDS
AATAAGGTGGTTGATAATAA+TGG	0.414023	8_3:+15135280	Msa1278670:CDS
AATAATGGTGGCAGCAACAA+AGG	0.529728	8_3:+15135295	Msa1278670:CDS
AATCCAATCCCTTCGAACAA+GGG	0.636829	8_3:-15135600	None:intergenic
AATGAATTCTATTATTATTC+TGG	0.162225	8_3:+15135421	Msa1278670:CDS
AATGGTGTTTGATGATAATA+AGG	0.374369	8_3:+15135264	Msa1278670:CDS
AATGTTGTCCCTTGTTCGAA+GGG	0.513132	8_3:+15135592	Msa1278670:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGAACAAGTGAATAGAAGA+GGG	-	chr8_3:15134633-15134652	None:intergenic	30.0%
TCATCTTCACTTCTACAAAA+TGG	+	chr8_3:15134649-15134668	Msa1278670:CDS	30.0%
TGGGTTTGAAAAATTTCAAC+TGG	-	chr8_3:15134682-15134701	None:intergenic	30.0%
CTTCTCTTGTCATATCTATT+AGG	+	chr8_3:15134789-15134808	Msa1278670:intron	30.0%
TTGTGAAAATATCTTGCTCT+TGG	-	chr8_3:15134979-15134998	None:intergenic	30.0%
GGATTATGTTGAAGATTATG+AGG	+	chr8_3:15135618-15135637	Msa1278670:CDS	30.0%
CTAAAATCGTTAATGTGAGA+AGG	+	chr8_3:15135706-15135725	Msa1278670:exon	30.0%
TGTATTGTTTGTTCTTATGG+TGG	+	chr8_3:15135802-15135821	Msa1278670:three_prime_UTR	30.0%
ATGAACAAGTGAATAGAAGA+GGG	-	chr8_3:15134633-15134652	None:intergenic	30.0%
TCATCTTCACTTCTACAAAA+TGG	+	chr8_3:15134649-15134668	Msa1278670:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	15134591	15135834	15134591	ID=Msa1278670;Name=Msa1278670
chr8_3	mRNA	15134591	15135834	15134591	ID=Msa1278670-mRNA-1;Parent=Msa1278670;Name=Msa1278670-mRNA-1;_AED=0.07;_eAED=0.07;_QI=10\|1\|1\|1\|1\|1\|3\|111\|252
chr8_3	exon	15134591	15134774	15134591	ID=Msa1278670-mRNA-1:exon:5864;Parent=Msa1278670-mRNA-1
chr8_3	exon	15134974	15135029	15134974	ID=Msa1278670-mRNA-1:exon:5865;Parent=Msa1278670-mRNA-1
chr8_3	exon	15135195	15135834	15135195	ID=Msa1278670-mRNA-1:exon:5866;Parent=Msa1278670-mRNA-1
chr8_3	five_prime_UTR	15134591	15134600	15134591	ID=Msa1278670-mRNA-1:five_prime_utr;Parent=Msa1278670-mRNA-1
chr8_3	CDS	15134601	15134774	15134601	ID=Msa1278670-mRNA-1:cds;Parent=Msa1278670-mRNA-1
chr8_3	CDS	15134974	15135029	15134974	ID=Msa1278670-mRNA-1:cds;Parent=Msa1278670-mRNA-1
chr8_3	CDS	15135195	15135723	15135195	ID=Msa1278670-mRNA-1:cds;Parent=Msa1278670-mRNA-1
chr8_3	three_prime_UTR	15135724	15135834	15135724	ID=Msa1278670-mRNA-1:three_prime_utr;Parent=Msa1278670-mRNA-1

Gene Sequence

>Msa1278670

ATGAGGATTCGGAAAAACACAGAGCTTTCCCCTCTTCTATTCACTTGTTCATCTTCACTTCTACAAAATGGTTCATTTCCAGTTGAAATTTTTCAAACCCATGTTTGTCAACTTAACCAATCTCCATGGGATGTGATCCCCTTTGCCTCCAACAACTCCTCCTCAATTCACCAGTTCCAAGAGCAAGATATTTTCACAAATGGGGATTCCTTTGGAGCTCTTGATGAAAGTGTGGATTCGTTGATGGAAACTGAAGTTGTTGATGCTGATAACAACAAACCTGCGATTGAAAATTATGAAATGGTGTTTGATGATAATAAGGTGGTTGATAATAATGGTGGCAGCAACAAAGGTTTGAAGAGAAACTCGAATGGGGGTGGCAGCATTGAGGCGCCGCGACGTTGTAGAGGCCGACCGAAGAAGTCATTGGTGGGTTCGGCTTCGGGTTCAAATAATAATGAATTCTATTATTATTCTGGGTTTGGTCCATCGCGGCGGAAACAACGAAAGGGTGATAGTAAAAGTGAAGGTGAAGATAACAAGAGTGTTGTGGAATTAGGTTCTACTAGTAGTGATAATGTTAAGGTTAATGTTGGTAATAATAATGTTGTTGCTAGTGTTGATGCTAATGTTGTCCCTTGTTCGAAGGGATTGGATTATGTTGAAGATTATGAGGATGATTATGATGATTCTGGTGATGATAATGGGAGGAGAACAAGGAAGCCAGTGAAAGAAAGGTCCCTAAAATCGTTAATGTGA

Protein sequence

>Msa1278670

MRIRKNTELSPLLFTCSSSLLQNGSFPVEIFQTHVCQLNQSPWDVIPFASNNSSSIHQFQEQDIFTNGDSFGALDESVDSLMETEVVDADNNKPAIENYEMVFDDNKVVDNNGGSNKGLKRNSNGGGSIEAPRRCRGRPKKSLVGSASGSNNNEFYYYSGFGPSRRKQRKGDSKSEGEDNKSVVELGSTSSDNVKVNVGNNNVVASVDANVVPCSKGLDYVEDYEDDYDDSGDDNGRRTRKPVKERSLKSLM*