AlfalfaGEDB | Msa1282990

M. sativa cultivar zhongmu-4 / Msa1282990

KEGG: K09422
GO: GO:0000003 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006996 GO:0007033 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008285 GO:0009555 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009735 GO:0009739 GO:0009740 GO:0009751 GO:0009755 GO:0009787 GO:0009789 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010476 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0014070 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022412 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033993 GO:0040008 GO:0042127 GO:0042221 GO:0042493 GO:0043067 GO:0043068 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0044703 GO:0045893 GO:0045926 GO:0045935 GO:0046677 GO:0048229 GO:0048232 GO:0048235 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048466 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048608 GO:0048609 GO:0048653 GO:0048654 GO:0048655 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051716 GO:0055046 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071840 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901419 GO:1901421 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1905957 GO:1905959 GO:1990019 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1282990	sp\|Q9FR97\|MYB65_ARATH	41.308	535	265	14	36	542	40	553	1.35e-107	335

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1282990	A0A072TNU8	96.886	546	17	0	1	546	1	546	0.0	1105

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TFs/TRs:

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Gene ID	Type	Classification
Msa1282990	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0424320	Msa1282990	-0.800822	1.203629e-48	-8.615850e-47
Msa0701010	Msa1282990	-0.801720	7.881015e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1282990	MtrunA17_Chr8g0354661	96.886	546	17	0	1	546	1	546	0.0	1105
Msa1282990	MtrunA17_Chr3g0080861	53.704	540	213	9	2	528	1	516	0.0	523
Msa1282990	MtrunA17_Chr3g0099131	81.739	115	21	0	37	151	41	155	3.00e-61	211
Msa1282990	MtrunA17_Chr2g0322521	76.860	121	28	0	37	157	29	149	1.20e-58	200
Msa1282990	MtrunA17_Chr1g0154031	70.968	124	36	0	37	160	76	199	7.72e-57	196
Msa1282990	MtrunA17_Chr1g0192411	64.912	114	40	0	35	148	10	123	3.49e-46	167
Msa1282990	MtrunA17_Chr8g0349261	66.667	105	34	1	37	140	13	117	1.90e-45	162
Msa1282990	MtrunA17_Chr5g0410961	62.264	106	40	0	35	140	10	115	9.14e-45	161
Msa1282990	MtrunA17_Chr1g0203411	60.331	121	46	1	33	153	10	128	1.42e-44	159
Msa1282990	MtrunA17_Chr4g0039971	66.346	104	35	0	37	140	13	116	3.26e-44	160

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1282990	AT3G11440.3	41.308	535	265	14	36	542	40	553	1.38e-108	335
Msa1282990	AT3G11440.4	41.308	535	265	14	36	542	40	553	1.38e-108	335
Msa1282990	AT3G11440.1	41.308	535	265	14	36	542	40	553	1.38e-108	335
Msa1282990	AT5G06100.5	39.925	531	260	15	34	545	29	519	1.23e-101	317
Msa1282990	AT5G06100.2	39.925	531	260	15	34	545	29	519	1.23e-101	317
Msa1282990	AT5G06100.3	39.925	531	260	15	34	545	29	519	1.23e-101	317
Msa1282990	AT5G06100.4	39.925	531	260	15	34	545	29	519	1.23e-101	317
Msa1282990	AT3G11440.2	47.354	378	172	9	36	388	40	415	2.31e-94	296
Msa1282990	AT5G06100.1	46.978	364	171	11	34	378	29	389	6.49e-88	278
Msa1282990	AT5G55020.1	80.000	120	24	0	34	153	23	142	5.97e-66	223

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Find 165 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACAAATGCAATTAGTAC+AGG	0.532480	8_3:+24120106	None:intergenic
AACTATAAACACATGACTGC+TGG	0.447793	8_3:-24120245	Msa1282990:CDS
AAGAAGAGAAAGAAAAGAAG+AGG	0.485281	8_3:+24124357	None:intergenic
AAGTCTATAAACCAGTGTAA+CGG	0.649430	8_3:+24120006	None:intergenic
AATACTTCAGGAGGATAAAG+TGG	0.587179	8_3:+24121741	None:intergenic
AATATTGAAGATGTGCCAAA+TGG	0.499766	8_3:+24120937	None:intergenic
AATGCTGTTCAGAAGCAGAC+AGG	0.553023	8_3:-24122614	Msa1282990:CDS
AATGTCCCTTTCTTTAAATT+TGG	0.223698	8_3:+24122539	None:intergenic
AATTCATCTACTGCAACTCC+TGG	0.580591	8_3:-24121030	Msa1282990:CDS
ACAATACCTGAATTAGAACG+TGG	0.712128	8_3:+24123964	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAAGAGAAAGAAAAGAAG+AGG	+	chr8_3:24119954-24119973	None:intergenic	30.0%
TTTACTAGGTTAGAAATTGG+GGG	+	chr8_3:24120198-24120217	None:intergenic	30.0%
GTTTACTAGGTTAGAAATTG+GGG	+	chr8_3:24120199-24120218	None:intergenic	30.0%
CAGTTTACTAGGTTAGAAAT+TGG	+	chr8_3:24120201-24120220	None:intergenic	30.0%
ATGACAGAATTTAGTGTGTT+TGG	-	chr8_3:24120738-24120757	Msa1282990:intron	30.0%
CCATACCCTAATTATGTTTA+GGG	+	chr8_3:24120870-24120889	None:intergenic	30.0%
TCCATACCCTAATTATGTTT+AGG	+	chr8_3:24120871-24120890	None:intergenic	30.0%
TTTGATACAGTAGACGAATA+TGG	-	chr8_3:24121064-24121083	Msa1282990:CDS	30.0%
TTGTTTCCATTTAAGTGCTT+TGG	-	chr8_3:24121278-24121297	Msa1282990:CDS	30.0%
GTCAATTATGTCAAGAAGAA+CGG	-	chr8_3:24121658-24121677	Msa1282990:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	24119896	24124434	24119896	ID=Msa1282990;Name=Msa1282990
chr8_3	mRNA	24119896	24124434	24119896	ID=Msa1282990-mRNA-1;Parent=Msa1282990;Name=Msa1282990-mRNA-1;_AED=0.01;_eAED=0.01;_QI=283\|1\|1\|1\|0.5\|0.4\|5\|261\|546
chr8_3	exon	24119896	24120464	24119896	ID=Msa1282990-mRNA-1:exon:8551;Parent=Msa1282990-mRNA-1
chr8_3	exon	24120866	24121838	24120866	ID=Msa1282990-mRNA-1:exon:8550;Parent=Msa1282990-mRNA-1
chr8_3	exon	24122463	24122838	24122463	ID=Msa1282990-mRNA-1:exon:8549;Parent=Msa1282990-mRNA-1
chr8_3	exon	24123971	24124075	24123971	ID=Msa1282990-mRNA-1:exon:8548;Parent=Msa1282990-mRNA-1
chr8_3	exon	24124273	24124434	24124273	ID=Msa1282990-mRNA-1:exon:8547;Parent=Msa1282990-mRNA-1
chr8_3	five_prime_UTR	24124273	24124434	24124273	ID=Msa1282990-mRNA-1:five_prime_utr;Parent=Msa1282990-mRNA-1
chr8_3	five_prime_UTR	24123971	24124075	24123971	ID=Msa1282990-mRNA-1:five_prime_utr;Parent=Msa1282990-mRNA-1
chr8_3	five_prime_UTR	24122823	24122838	24122823	ID=Msa1282990-mRNA-1:five_prime_utr;Parent=Msa1282990-mRNA-1
chr8_3	CDS	24122463	24122822	24122463	ID=Msa1282990-mRNA-1:cds;Parent=Msa1282990-mRNA-1
chr8_3	CDS	24120866	24121838	24120866	ID=Msa1282990-mRNA-1:cds;Parent=Msa1282990-mRNA-1
chr8_3	CDS	24120157	24120464	24120157	ID=Msa1282990-mRNA-1:cds;Parent=Msa1282990-mRNA-1
chr8_3	three_prime_UTR	24119896	24120156	24119896	ID=Msa1282990-mRNA-1:three_prime_utr;Parent=Msa1282990-mRNA-1

Gene Sequence

>Msa1282990

ATGATGAGGCGGATGAAAAAGGAGATTGAGGATGAGATGCTCGACGATGATCGGATTGGGTCAGAGTTTAATGATGAGGAGTATGACAGAAGTGCCCATGGAGTTCTTCTAAAGAAAGGGCCATGGACATCTGCTGAAGACGATATTTTGGTCAATTATGTCAAGAAGAACGGAGAGGGGAACTGGAATGCTGTTCAGAAGCAGACAGGCCTGCTACGTTGCGGAAAAAGTTGTCGATTGCGGTGGGCTAATCACCTTAGGCCAAATTTAAAGAAAGGGACATTTACTGCAGAAGAGGAGCAGTTGATTTGTGAGCTTCATGCAAAATTGGGAAATAAATGGGCACGCATGGCAGCACATTTACCTGGTCGTACAGATAACGAGATAAAGAACTATTGGAACACTCGGATCAAGAGGCGTCACCGGGCTGGCTTGCCACTTTATCCTCCTGAAGTATTGCAACAGACTCTTCATGGCCAAAGCACCGATGGAATTAACAGCGGCAACAAAGTGCATCCTGATTTCTTGCAGAAAAAGAATTTTGATATACATGATGCAATATATGACTGTATGAAGGATCACCAGGGAATGTTACCTTATGTTGCTGAGCTTCCTGATATTTCTGCTTATAGCAATATGCTAAATGGTTTTGATTCTTCTCAGTATTGTAGTTTTGGGCAATCAACATCATCTAACCACAAACATTTTCGAGATTCAACAATTCCATTCCTGGATTTCAGTTGTATCGACAGAAATGGTTATTATCCATTTGATCATATTCAGGATGATACTTTTTATAAGTGGACACAATCATTTGGCATGCAATCACCCCTTCATCATCCTGGTCCCTCCTCTCACAGCTCAATGTGTTACAGCCATTCACTAAAAAATGGAAATTCCTCCACTTCTATGCCTTTCGAGGAGCTTCCTTCAATCCAATATCCAGAAACTGATTTAGGTAGCTGGGGCACATCTCCCCCACCTCCTTTACTTGAGTCAGTTGATGATTTCATTAAGTCTCCTACACCAATTAGTACACTGGAGTCAGATTGTTCTTCTCCACAGAATAGTGGCCTGCTACAATCTGTACTTTATCCGCGGAAGACTCCAAGCAGTTCAAAAAACCAATTTTATGTCAAGAGTTCAAATTCATCTACTGCAACTCCTGGTGAAAGAGCAGGCAGCTCTGCTTTGAACATGTATGAGACAGAATTGGAAGAATGTGCTGACCCTGTATCTCCATTTGGCACATCTTCAATATTGAACGAGTGCCCTGCTGCTAGTGCCAACAGAAGTTCACTGGATGAGCAGCCACCAGTTCATACCGTTAATGGCAACACTGTGAAAGTAGAGTATGTTGACCCTGTTTGGACCCCTGACAGTGGAAATCAAATCACGTCTCTGTCGAACTTTAGTCGGCCAGATTTCTTGCTTGGTTCAGATTGGTATGAGCAATGTTCTGGGAATGACCAGAACCAATCCATCTTCTATGATGCTGCATCGATGTTTCTTGAAGATCAATCAGCCACCAACTATAAACACATGACTGCTGGAACTTCTAACCCAAGTCAGGTAAGGGAGTTTGGTTCGTGTGCATGGAACAACATGCCTGCTGTCTGTCAATTATCTGATCTTGGTTAA

Protein sequence

>Msa1282990

MMRRMKKEIEDEMLDDDRIGSEFNDEEYDRSAHGVLLKKGPWTSAEDDILVNYVKKNGEGNWNAVQKQTGLLRCGKSCRLRWANHLRPNLKKGTFTAEEEQLICELHAKLGNKWARMAAHLPGRTDNEIKNYWNTRIKRRHRAGLPLYPPEVLQQTLHGQSTDGINSGNKVHPDFLQKKNFDIHDAIYDCMKDHQGMLPYVAELPDISAYSNMLNGFDSSQYCSFGQSTSSNHKHFRDSTIPFLDFSCIDRNGYYPFDHIQDDTFYKWTQSFGMQSPLHHPGPSSHSSMCYSHSLKNGNSSTSMPFEELPSIQYPETDLGSWGTSPPPPLLESVDDFIKSPTPISTLESDCSSPQNSGLLQSVLYPRKTPSSSKNQFYVKSSNSSTATPGERAGSSALNMYETELEECADPVSPFGTSSILNECPAASANRSSLDEQPPVHTVNGNTVKVEYVDPVWTPDSGNQITSLSNFSRPDFLLGSDWYEQCSGNDQNQSIFYDAASMFLEDQSATNYKHMTAGTSNPSQVREFGSCAWNNMPAVCQLSDLG*