AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1285810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTGTTGTAAGATTGTATT+TGG 0.256741 8_3:+32058668 Msa1285810:exon
TCATAACTCTGGAAAGATTA+TGG 0.277813 8_3:+32058609 Msa1285810:CDS
TTTGTATGGCTTGATGATTT+TGG 0.287612 8_3:+32058637 Msa1285810:CDS
CACGACTGGTTCACAGTAGA+TGG 0.325624 8_3:+32057086 Msa1285810:CDS
GCAAGAGTTGTCATGGCTCT+TGG 0.427467 8_3:+32057037 None:intergenic
CATGGAACTGTGACTATGTT+GGG 0.434410 8_3:+32058843 Msa1285810:three_prime_UTR
TTATGTTCCCATTCTGTATG+TGG 0.445861 8_3:+32058753 Msa1285810:three_prime_UTR
GATTGTATTTGGTTCCGAAC+TGG 0.452309 8_3:+32058679 Msa1285810:three_prime_UTR
TCATGGAACTGTGACTATGT+TGG 0.492322 8_3:+32058842 Msa1285810:three_prime_UTR
TTTCCTGGATGTCATAACTC+TGG 0.495770 8_3:+32058598 Msa1285810:CDS
CCATTCTGTATGTGGGAGAT+CGG 0.508496 8_3:+32058761 Msa1285810:three_prime_UTR
CCGATCTCCCACATACAGAA+TGG 0.509478 8_3:-32058761 None:intergenic
CGATCTCCCACATACAGAAT+GGG 0.519174 8_3:-32058760 None:intergenic
TTTCCAGAGTTATGACATCC+AGG 0.523368 8_3:-32058601 None:intergenic
TATGTTCCCATTCTGTATGT+GGG 0.539690 8_3:+32058754 Msa1285810:three_prime_UTR
AACTCTGGAAAGATTATGGA+TGG 0.540930 8_3:+32058613 Msa1285810:CDS
CTATGTTGGGCTCAGTATGT+TGG 0.543469 8_3:+32058856 Msa1285810:three_prime_UTR
TGAAAGAGTATATACCAGTT+CGG 0.551003 8_3:-32058693 None:intergenic
GGAATGAGAATAAAGAGGCT+TGG 0.568608 8_3:+32057058 Msa1285810:CDS
ACAATCTTACAACAGCCTAA+AGG 0.570617 8_3:-32058662 None:intergenic
CAGTAGATGGGAAAGTGAAG+AGG 0.599747 8_3:+32057099 Msa1285810:CDS
CATCTACTGTGAACCAGTCG+TGG 0.602366 8_3:-32057085 None:intergenic
GGGCTCAGTATGTTGGTGCT+AGG 0.603068 8_3:+32058863 Msa1285810:three_prime_UTR
TACTGTGAACCAGTCGTGGA+CGG 0.606935 8_3:-32057081 None:intergenic
ACGACTGGTTCACAGTAGAT+GGG 0.616977 8_3:+32057087 Msa1285810:CDS
CTCTTGGAATGAGAATAAAG+AGG 0.658215 8_3:+32057053 Msa1285810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAACTCCATAATTAT+TGG + chr8_3:32057755-32057774 Msa1285810:intron 15.0%
!!! AGATATTTAGTTTGATTTAT+AGG + chr8_3:32058049-32058068 Msa1285810:intron 15.0%
!! AAAATTAACTCCATAATTAT+TGG + chr8_3:32057755-32057774 Msa1285810:intron 15.0%
!!! AGATATTTAGTTTGATTTAT+AGG + chr8_3:32058049-32058068 Msa1285810:intron 15.0%
!!! TTTAGTTTGATTTATAGGAT+TGG + chr8_3:32058054-32058073 Msa1285810:intron 20.0%
!!! CATTTACTTTTATTGTTCTT+CGG + chr8_3:32058379-32058398 Msa1285810:intron 20.0%
!!! CATTTACTTTTATTGTTCTT+GGG + chr8_3:32058379-32058398 Msa1285810:intron 20.0%
!! AATTATGAAAAACTATTTGC+TGG + chr8_3:32058819-32058838 Msa1285810:three_prime_UTR 20.0%
!!! TTTAGTTTGATTTATAGGAT+TGG + chr8_3:32058054-32058073 Msa1285810:intron 20.0%
!!! CATTTACTTTTATTGTTCTT+CGG + chr8_3:32058379-32058398 Msa1285810:intron 20.0%
!!! CATTTACTTTTATTGTTCTT+GGG + chr8_3:32058379-32058398 Msa1285810:intron 20.0%
!! AATTATGAAAAACTATTTGC+TGG + chr8_3:32058819-32058838 Msa1285810:three_prime_UTR 20.0%
! CATATGTTGTAAGTCAAATA+TGG + chr8_3:32057486-32057505 Msa1285810:intron 25.0%
! ATAATATATTAGAAGAGAGC+AGG - chr8_3:32057618-32057637 None:intergenic 25.0%
! AATCTTTGATGCATTTATGT+TGG + chr8_3:32057721-32057740 Msa1285810:intron 25.0%
! TTAACTCCATAATTATTGGT+TGG + chr8_3:32057759-32057778 Msa1285810:intron 25.0%
!! TGGTGTTTGATAGATTTTAA+TGG + chr8_3:32058074-32058093 Msa1285810:intron 25.0%
!!! TACATTACTTTTTACACCTT+TGG + chr8_3:32058108-32058127 Msa1285810:intron 25.0%
!! ACCTTTGGCTTATTAATATA+TGG + chr8_3:32058123-32058142 Msa1285810:intron 25.0%
! ACCATATATTAATAAGCCAA+AGG - chr8_3:32058127-32058146 None:intergenic 25.0%
! AATATATTCCAACATTGTCT+CGG - chr8_3:32058150-32058169 None:intergenic 25.0%
! TTAAACTTTATAGCAACTCT+TGG + chr8_3:32058179-32058198 Msa1285810:intron 25.0%
! TAAACTTTATAGCAACTCTT+GGG + chr8_3:32058180-32058199 Msa1285810:intron 25.0%
! AAGTATTTCATAGGAAAGTA+TGG - chr8_3:32058327-32058346 None:intergenic 25.0%
! TCCTATGAAATACTTTCAAA+TGG + chr8_3:32058332-32058351 Msa1285810:intron 25.0%
!!! ACCATTTGAAAGTATTTCAT+AGG - chr8_3:32058336-32058355 None:intergenic 25.0%
! CGAAGAACAATAAAAGTAAA+TGG - chr8_3:32058381-32058400 None:intergenic 25.0%
! AAGTATTTCATAGGAAAGTA+TGG - chr8_3:32058327-32058346 None:intergenic 25.0%
! TCCTATGAAATACTTTCAAA+TGG + chr8_3:32058332-32058351 Msa1285810:intron 25.0%
!!! ACCATTTGAAAGTATTTCAT+AGG - chr8_3:32058336-32058355 None:intergenic 25.0%
! CCAAGAACAATAAAAGTAAA+TGG - chr8_3:32058474-32058493 None:intergenic 25.0%
!!! CCATTTACTTTTATTGTTCT+TGG + chr8_3:32058378-32058397 Msa1285810:intron 25.0%
!! ATATTTCCAACAATCCATTT+TGG - chr8_3:32058530-32058549 None:intergenic 25.0%
! CATATGTTGTAAGTCAAATA+TGG + chr8_3:32057486-32057505 Msa1285810:intron 25.0%
! ATAATATATTAGAAGAGAGC+AGG - chr8_3:32057618-32057637 None:intergenic 25.0%
! AATCTTTGATGCATTTATGT+TGG + chr8_3:32057721-32057740 Msa1285810:intron 25.0%
! TTAACTCCATAATTATTGGT+TGG + chr8_3:32057759-32057778 Msa1285810:intron 25.0%
!! TGGTGTTTGATAGATTTTAA+TGG + chr8_3:32058074-32058093 Msa1285810:intron 25.0%
!!! TACATTACTTTTTACACCTT+TGG + chr8_3:32058108-32058127 Msa1285810:intron 25.0%
!! ACCTTTGGCTTATTAATATA+TGG + chr8_3:32058123-32058142 Msa1285810:intron 25.0%
! ACCATATATTAATAAGCCAA+AGG - chr8_3:32058127-32058146 None:intergenic 25.0%
! AATATATTCCAACATTGTCT+CGG - chr8_3:32058150-32058169 None:intergenic 25.0%
! TTAAACTTTATAGCAACTCT+TGG + chr8_3:32058179-32058198 Msa1285810:intron 25.0%
! TAAACTTTATAGCAACTCTT+GGG + chr8_3:32058180-32058199 Msa1285810:intron 25.0%
! AAGTATTTCATAGGAAAGTA+TGG - chr8_3:32058327-32058346 None:intergenic 25.0%
! TCCTATGAAATACTTTCAAA+TGG + chr8_3:32058332-32058351 Msa1285810:intron 25.0%
!!! ACCATTTGAAAGTATTTCAT+AGG - chr8_3:32058336-32058355 None:intergenic 25.0%
! CGAAGAACAATAAAAGTAAA+TGG - chr8_3:32058381-32058400 None:intergenic 25.0%
! AAGTATTTCATAGGAAAGTA+TGG - chr8_3:32058327-32058346 None:intergenic 25.0%
! TCCTATGAAATACTTTCAAA+TGG + chr8_3:32058332-32058351 Msa1285810:intron 25.0%
!!! ACCATTTGAAAGTATTTCAT+AGG - chr8_3:32058336-32058355 None:intergenic 25.0%
! CCAAGAACAATAAAAGTAAA+TGG - chr8_3:32058474-32058493 None:intergenic 25.0%
!!! CCATTTACTTTTATTGTTCT+TGG + chr8_3:32058378-32058397 Msa1285810:intron 25.0%
!! ATATTTCCAACAATCCATTT+TGG - chr8_3:32058530-32058549 None:intergenic 25.0%
! GTCATTACAGTGGTATTTTA+AGG - chr8_3:32057193-32057212 None:intergenic 30.0%
TAAAAACTGAGTCATTACAG+TGG - chr8_3:32057203-32057222 None:intergenic 30.0%
! ACTGTAATGACTCAGTTTTT+AGG + chr8_3:32057202-32057221 Msa1285810:intron 30.0%
! CTGTAATGACTCAGTTTTTA+GGG + chr8_3:32057203-32057222 Msa1285810:intron 30.0%
TAACCTATGTTGTCAATAGT+GGG - chr8_3:32057407-32057426 None:intergenic 30.0%
TTAACCTATGTTGTCAATAG+TGG - chr8_3:32057408-32057427 None:intergenic 30.0%
GACAACATAGGTTAAATATG+TGG + chr8_3:32057413-32057432 Msa1285810:intron 30.0%
! TCCATAATTATTGGTTGGTT+TGG + chr8_3:32057764-32057783 Msa1285810:intron 30.0%
ACCAAACCAACCAATAATTA+TGG - chr8_3:32057768-32057787 None:intergenic 30.0%
AGGTATTTATATGTCATAGC+AGG - chr8_3:32057862-32057881 None:intergenic 30.0%
CATCTGCAATATCTATAAGT+CGG - chr8_3:32057951-32057970 None:intergenic 30.0%
AAATCACCCCAATTATTGTA+GGG + chr8_3:32058205-32058224 Msa1285810:intron 30.0%
!! CATTATTTTGCAAGCAAAAC+AGG - chr8_3:32058276-32058295 None:intergenic 30.0%
! TTTCATAGGAAAGTATGGAA+GGG - chr8_3:32058322-32058341 None:intergenic 30.0%
! ATTTCATAGGAAAGTATGGA+AGG - chr8_3:32058323-32058342 None:intergenic 30.0%
! TTTCATAGGAAAGTATGGAA+GGG - chr8_3:32058322-32058341 None:intergenic 30.0%
! ATTTCATAGGAAAGTATGGA+AGG - chr8_3:32058323-32058342 None:intergenic 30.0%
! TCATAACTCTGGAAAGATTA+TGG + chr8_3:32058609-32058628 Msa1285810:CDS 30.0%
!!! AGATTATGGATGGTTTTGTA+TGG + chr8_3:32058623-32058642 Msa1285810:CDS 30.0%
!! TTTGTATGGCTTGATGATTT+TGG + chr8_3:32058637-32058656 Msa1285810:CDS 30.0%
!!! TTGATGATTTTGGTTCCTTT+AGG + chr8_3:32058647-32058666 Msa1285810:CDS 30.0%
! TGAAAGAGTATATACCAGTT+CGG - chr8_3:32058696-32058715 None:intergenic 30.0%
!! AATTTGCTTGTGACTGTTTT+TGG + chr8_3:32058728-32058747 Msa1285810:three_prime_UTR 30.0%
!! ATTTGCTTGTGACTGTTTTT+GGG + chr8_3:32058729-32058748 Msa1285810:three_prime_UTR 30.0%
! GTCATTACAGTGGTATTTTA+AGG - chr8_3:32057193-32057212 None:intergenic 30.0%
TAAAAACTGAGTCATTACAG+TGG - chr8_3:32057203-32057222 None:intergenic 30.0%
! ACTGTAATGACTCAGTTTTT+AGG + chr8_3:32057202-32057221 Msa1285810:intron 30.0%
! CTGTAATGACTCAGTTTTTA+GGG + chr8_3:32057203-32057222 Msa1285810:intron 30.0%
TAACCTATGTTGTCAATAGT+GGG - chr8_3:32057407-32057426 None:intergenic 30.0%
TTAACCTATGTTGTCAATAG+TGG - chr8_3:32057408-32057427 None:intergenic 30.0%
GACAACATAGGTTAAATATG+TGG + chr8_3:32057413-32057432 Msa1285810:intron 30.0%
! TCCATAATTATTGGTTGGTT+TGG + chr8_3:32057764-32057783 Msa1285810:intron 30.0%
ACCAAACCAACCAATAATTA+TGG - chr8_3:32057768-32057787 None:intergenic 30.0%
AGGTATTTATATGTCATAGC+AGG - chr8_3:32057862-32057881 None:intergenic 30.0%
CATCTGCAATATCTATAAGT+CGG - chr8_3:32057951-32057970 None:intergenic 30.0%
AAATCACCCCAATTATTGTA+GGG + chr8_3:32058205-32058224 Msa1285810:intron 30.0%
!! CATTATTTTGCAAGCAAAAC+AGG - chr8_3:32058276-32058295 None:intergenic 30.0%
! TTTCATAGGAAAGTATGGAA+GGG - chr8_3:32058322-32058341 None:intergenic 30.0%
! ATTTCATAGGAAAGTATGGA+AGG - chr8_3:32058323-32058342 None:intergenic 30.0%
! TTTCATAGGAAAGTATGGAA+GGG - chr8_3:32058322-32058341 None:intergenic 30.0%
! ATTTCATAGGAAAGTATGGA+AGG - chr8_3:32058323-32058342 None:intergenic 30.0%
! TCATAACTCTGGAAAGATTA+TGG + chr8_3:32058609-32058628 Msa1285810:CDS 30.0%
!!! AGATTATGGATGGTTTTGTA+TGG + chr8_3:32058623-32058642 Msa1285810:CDS 30.0%
!! TTTGTATGGCTTGATGATTT+TGG + chr8_3:32058637-32058656 Msa1285810:CDS 30.0%
!!! TTGATGATTTTGGTTCCTTT+AGG + chr8_3:32058647-32058666 Msa1285810:CDS 30.0%
! TGAAAGAGTATATACCAGTT+CGG - chr8_3:32058696-32058715 None:intergenic 30.0%
!! AATTTGCTTGTGACTGTTTT+TGG + chr8_3:32058728-32058747 Msa1285810:three_prime_UTR 30.0%
!! ATTTGCTTGTGACTGTTTTT+GGG + chr8_3:32058729-32058748 Msa1285810:three_prime_UTR 30.0%
CTCTTGGAATGAGAATAAAG+AGG + chr8_3:32057053-32057072 Msa1285810:CDS 35.0%
! AAAGAAGAAGTGTGTGTTTC+TGG + chr8_3:32057122-32057141 Msa1285810:intron 35.0%
ACAGTGAAGAGAGATATACT+GGG + chr8_3:32057147-32057166 Msa1285810:intron 35.0%
! ATTTGCTTTGCGTTTTACAG+TGG - chr8_3:32057273-32057292 None:intergenic 35.0%
TGTAAGTCAAATATGGAAGC+TGG + chr8_3:32057493-32057512 Msa1285810:intron 35.0%
! TGCATTTTATCTGCATTGCT+TGG + chr8_3:32057563-32057582 Msa1285810:intron 35.0%
! TACATGTCATTTCAAGACAC+TGG + chr8_3:32057683-32057702 Msa1285810:intron 35.0%
GATAGTTGAGGTGATGAATT+GGG - chr8_3:32057809-32057828 None:intergenic 35.0%
AGATAGTTGAGGTGATGAAT+TGG - chr8_3:32057810-32057829 None:intergenic 35.0%
ATGTCATAGCAGGTACAAAA+AGG - chr8_3:32057852-32057871 None:intergenic 35.0%
CACTGACAAACAACAAAACA+AGG - chr8_3:32057882-32057901 None:intergenic 35.0%
!!! CTTGTTTTGTTGTTTGTCAG+TGG + chr8_3:32057880-32057899 Msa1285810:intron 35.0%
!!! TTTTGTTGTTTGTCAGTGGA+TGG + chr8_3:32057884-32057903 Msa1285810:intron 35.0%
!! TTTGTTGTTTGTCAGTGGAT+GGG + chr8_3:32057885-32057904 Msa1285810:intron 35.0%
GGATCTTGCTTATTATCCTT+TGG + chr8_3:32057906-32057925 Msa1285810:intron 35.0%
GCATTATTTGCTCAAACCAA+AGG - chr8_3:32057925-32057944 None:intergenic 35.0%
CGACTTATAGATATTGCAGA+TGG + chr8_3:32057949-32057968 Msa1285810:intron 35.0%
TCTTGTTCAAGTGTGTAGTT+TGG + chr8_3:32057991-32058010 Msa1285810:intron 35.0%
CTACAATAATTGGGGTGATT+TGG - chr8_3:32058206-32058225 None:intergenic 35.0%
CAAATCACCCCAATTATTGT+AGG + chr8_3:32058204-32058223 Msa1285810:intron 35.0%
AATCACCCCAATTATTGTAG+GGG + chr8_3:32058206-32058225 Msa1285810:intron 35.0%
TTTGCTCCCCTACAATAATT+GGG - chr8_3:32058215-32058234 None:intergenic 35.0%
! TTTTGCTCCCCTACAATAAT+TGG - chr8_3:32058216-32058235 None:intergenic 35.0%
GGCTATAAGTCTTTCTTGTT+TGG - chr8_3:32058301-32058320 None:intergenic 35.0%
AGAATGTACCTGATCCAAAA+TGG + chr8_3:32058513-32058532 Msa1285810:intron 35.0%
! AACTCTGGAAAGATTATGGA+TGG + chr8_3:32058613-32058632 Msa1285810:CDS 35.0%
ACAATCTTACAACAGCCTAA+AGG - chr8_3:32058665-32058684 None:intergenic 35.0%
GGCTGTTGTAAGATTGTATT+TGG + chr8_3:32058668-32058687 Msa1285810:exon 35.0%
!!! TTTGCTTGTGACTGTTTTTG+GGG + chr8_3:32058730-32058749 Msa1285810:three_prime_UTR 35.0%
TTATGTTCCCATTCTGTATG+TGG + chr8_3:32058753-32058772 Msa1285810:three_prime_UTR 35.0%
TATGTTCCCATTCTGTATGT+GGG + chr8_3:32058754-32058773 Msa1285810:three_prime_UTR 35.0%
! GAAAAACTATTTGCTGGTCA+TGG + chr8_3:32058825-32058844 Msa1285810:three_prime_UTR 35.0%
CTCTTGGAATGAGAATAAAG+AGG + chr8_3:32057053-32057072 Msa1285810:CDS 35.0%
! AAAGAAGAAGTGTGTGTTTC+TGG + chr8_3:32057122-32057141 Msa1285810:intron 35.0%
ACAGTGAAGAGAGATATACT+GGG + chr8_3:32057147-32057166 Msa1285810:intron 35.0%
! ATTTGCTTTGCGTTTTACAG+TGG - chr8_3:32057273-32057292 None:intergenic 35.0%
TGTAAGTCAAATATGGAAGC+TGG + chr8_3:32057493-32057512 Msa1285810:intron 35.0%
! TGCATTTTATCTGCATTGCT+TGG + chr8_3:32057563-32057582 Msa1285810:intron 35.0%
! TACATGTCATTTCAAGACAC+TGG + chr8_3:32057683-32057702 Msa1285810:intron 35.0%
GATAGTTGAGGTGATGAATT+GGG - chr8_3:32057809-32057828 None:intergenic 35.0%
AGATAGTTGAGGTGATGAAT+TGG - chr8_3:32057810-32057829 None:intergenic 35.0%
ATGTCATAGCAGGTACAAAA+AGG - chr8_3:32057852-32057871 None:intergenic 35.0%
CACTGACAAACAACAAAACA+AGG - chr8_3:32057882-32057901 None:intergenic 35.0%
!!! CTTGTTTTGTTGTTTGTCAG+TGG + chr8_3:32057880-32057899 Msa1285810:intron 35.0%
!!! TTTTGTTGTTTGTCAGTGGA+TGG + chr8_3:32057884-32057903 Msa1285810:intron 35.0%
!! TTTGTTGTTTGTCAGTGGAT+GGG + chr8_3:32057885-32057904 Msa1285810:intron 35.0%
GGATCTTGCTTATTATCCTT+TGG + chr8_3:32057906-32057925 Msa1285810:intron 35.0%
GCATTATTTGCTCAAACCAA+AGG - chr8_3:32057925-32057944 None:intergenic 35.0%
CGACTTATAGATATTGCAGA+TGG + chr8_3:32057949-32057968 Msa1285810:intron 35.0%
TCTTGTTCAAGTGTGTAGTT+TGG + chr8_3:32057991-32058010 Msa1285810:intron 35.0%
CTACAATAATTGGGGTGATT+TGG - chr8_3:32058206-32058225 None:intergenic 35.0%
CAAATCACCCCAATTATTGT+AGG + chr8_3:32058204-32058223 Msa1285810:intron 35.0%
AATCACCCCAATTATTGTAG+GGG + chr8_3:32058206-32058225 Msa1285810:intron 35.0%
TTTGCTCCCCTACAATAATT+GGG - chr8_3:32058215-32058234 None:intergenic 35.0%
! TTTTGCTCCCCTACAATAAT+TGG - chr8_3:32058216-32058235 None:intergenic 35.0%
GGCTATAAGTCTTTCTTGTT+TGG - chr8_3:32058301-32058320 None:intergenic 35.0%
AGAATGTACCTGATCCAAAA+TGG + chr8_3:32058513-32058532 Msa1285810:intron 35.0%
! AACTCTGGAAAGATTATGGA+TGG + chr8_3:32058613-32058632 Msa1285810:CDS 35.0%
ACAATCTTACAACAGCCTAA+AGG - chr8_3:32058665-32058684 None:intergenic 35.0%
GGCTGTTGTAAGATTGTATT+TGG + chr8_3:32058668-32058687 Msa1285810:exon 35.0%
!!! TTTGCTTGTGACTGTTTTTG+GGG + chr8_3:32058730-32058749 Msa1285810:three_prime_UTR 35.0%
TTATGTTCCCATTCTGTATG+TGG + chr8_3:32058753-32058772 Msa1285810:three_prime_UTR 35.0%
TATGTTCCCATTCTGTATGT+GGG + chr8_3:32058754-32058773 Msa1285810:three_prime_UTR 35.0%
! GAAAAACTATTTGCTGGTCA+TGG + chr8_3:32058825-32058844 Msa1285810:three_prime_UTR 35.0%
GGAATGAGAATAAAGAGGCT+TGG + chr8_3:32057058-32057077 Msa1285810:CDS 40.0%
CACAGTGAAGAGAGATATAC+TGG + chr8_3:32057146-32057165 Msa1285810:intron 40.0%
AAACCCCCCTAACACAATAA+CGG + chr8_3:32057231-32057250 Msa1285810:intron 40.0%
ACAGGACCGTTATTGTGTTA+GGG - chr8_3:32057240-32057259 None:intergenic 40.0%
AACAGGACCGTTATTGTGTT+AGG - chr8_3:32057241-32057260 None:intergenic 40.0%
TACAGTGGTGACACAATAAC+AGG - chr8_3:32057258-32057277 None:intergenic 40.0%
CTTTGCATTGCTACATGTGT+GGG + chr8_3:32057326-32057345 Msa1285810:intron 40.0%
ACATTGTAGCCGATAGTACT+GGG - chr8_3:32057376-32057395 None:intergenic 40.0%
TAGCCCACTATTGACAACAT+AGG + chr8_3:32057401-32057420 Msa1285810:intron 40.0%
! ATGTCATTTCAAGACACTGG+TGG + chr8_3:32057686-32057705 Msa1285810:intron 40.0%
!! GTTTGGTGCAGAAGATTAGT+GGG + chr8_3:32057781-32057800 Msa1285810:intron 40.0%
GCAGGTACAAAAAGGAGAAA+GGG - chr8_3:32057844-32057863 None:intergenic 40.0%
AGCAGGTACAAAAAGGAGAA+AGG - chr8_3:32057845-32057864 None:intergenic 40.0%
! TGTGTAGTTTGGTTCAAACC+AGG + chr8_3:32058002-32058021 Msa1285810:intron 40.0%
! GTGTAGTTTGGTTCAAACCA+GGG + chr8_3:32058003-32058022 Msa1285810:intron 40.0%
TATATGGTCCGAGACAATGT+TGG + chr8_3:32058139-32058158 Msa1285810:intron 40.0%
TTGCTCCCCTACAATAATTG+GGG - chr8_3:32058214-32058233 None:intergenic 40.0%
TTGCAAGCAAAACAGGAGTA+GGG - chr8_3:32058269-32058288 None:intergenic 40.0%
TTTGCAAGCAAAACAGGAGT+AGG - chr8_3:32058270-32058289 None:intergenic 40.0%
! CCAACAATCCATTTTGGATC+AGG - chr8_3:32058524-32058543 None:intergenic 40.0%
!! CCTGATCCAAAATGGATTGT+TGG + chr8_3:32058521-32058540 Msa1285810:intron 40.0%
!!! TGCACTTTAGAGCATTTTCC+TGG + chr8_3:32058583-32058602 Msa1285810:CDS 40.0%
TTTCCTGGATGTCATAACTC+TGG + chr8_3:32058598-32058617 Msa1285810:CDS 40.0%
TTTCCAGAGTTATGACATCC+AGG - chr8_3:32058604-32058623 None:intergenic 40.0%
! GATTGTATTTGGTTCCGAAC+TGG + chr8_3:32058679-32058698 Msa1285810:three_prime_UTR 40.0%
TCATGGAACTGTGACTATGT+TGG + chr8_3:32058842-32058861 Msa1285810:three_prime_UTR 40.0%
CATGGAACTGTGACTATGTT+GGG + chr8_3:32058843-32058862 Msa1285810:three_prime_UTR 40.0%
GGAATGAGAATAAAGAGGCT+TGG + chr8_3:32057058-32057077 Msa1285810:CDS 40.0%
CACAGTGAAGAGAGATATAC+TGG + chr8_3:32057146-32057165 Msa1285810:intron 40.0%
AAACCCCCCTAACACAATAA+CGG + chr8_3:32057231-32057250 Msa1285810:intron 40.0%
ACAGGACCGTTATTGTGTTA+GGG - chr8_3:32057240-32057259 None:intergenic 40.0%
AACAGGACCGTTATTGTGTT+AGG - chr8_3:32057241-32057260 None:intergenic 40.0%
TACAGTGGTGACACAATAAC+AGG - chr8_3:32057258-32057277 None:intergenic 40.0%
CTTTGCATTGCTACATGTGT+GGG + chr8_3:32057326-32057345 Msa1285810:intron 40.0%
ACATTGTAGCCGATAGTACT+GGG - chr8_3:32057376-32057395 None:intergenic 40.0%
TAGCCCACTATTGACAACAT+AGG + chr8_3:32057401-32057420 Msa1285810:intron 40.0%
! ATGTCATTTCAAGACACTGG+TGG + chr8_3:32057686-32057705 Msa1285810:intron 40.0%
!! GTTTGGTGCAGAAGATTAGT+GGG + chr8_3:32057781-32057800 Msa1285810:intron 40.0%
GCAGGTACAAAAAGGAGAAA+GGG - chr8_3:32057844-32057863 None:intergenic 40.0%
AGCAGGTACAAAAAGGAGAA+AGG - chr8_3:32057845-32057864 None:intergenic 40.0%
! TGTGTAGTTTGGTTCAAACC+AGG + chr8_3:32058002-32058021 Msa1285810:intron 40.0%
! GTGTAGTTTGGTTCAAACCA+GGG + chr8_3:32058003-32058022 Msa1285810:intron 40.0%
TATATGGTCCGAGACAATGT+TGG + chr8_3:32058139-32058158 Msa1285810:intron 40.0%
TTGCTCCCCTACAATAATTG+GGG - chr8_3:32058214-32058233 None:intergenic 40.0%
TTGCAAGCAAAACAGGAGTA+GGG - chr8_3:32058269-32058288 None:intergenic 40.0%
TTTGCAAGCAAAACAGGAGT+AGG - chr8_3:32058270-32058289 None:intergenic 40.0%
! CCAACAATCCATTTTGGATC+AGG - chr8_3:32058524-32058543 None:intergenic 40.0%
!! CCTGATCCAAAATGGATTGT+TGG + chr8_3:32058521-32058540 Msa1285810:intron 40.0%
!!! TGCACTTTAGAGCATTTTCC+TGG + chr8_3:32058583-32058602 Msa1285810:CDS 40.0%
TTTCCTGGATGTCATAACTC+TGG + chr8_3:32058598-32058617 Msa1285810:CDS 40.0%
TTTCCAGAGTTATGACATCC+AGG - chr8_3:32058604-32058623 None:intergenic 40.0%
! GATTGTATTTGGTTCCGAAC+TGG + chr8_3:32058679-32058698 Msa1285810:three_prime_UTR 40.0%
TCATGGAACTGTGACTATGT+TGG + chr8_3:32058842-32058861 Msa1285810:three_prime_UTR 40.0%
CATGGAACTGTGACTATGTT+GGG + chr8_3:32058843-32058862 Msa1285810:three_prime_UTR 40.0%
! ACGACTGGTTCACAGTAGAT+GGG + chr8_3:32057087-32057106 Msa1285810:CDS 45.0%
CAGTAGATGGGAAAGTGAAG+AGG + chr8_3:32057099-32057118 Msa1285810:CDS 45.0%
AGGACCGTTATTGTGTTAGG+GGG - chr8_3:32057238-32057257 None:intergenic 45.0%
CAGGACCGTTATTGTGTTAG+GGG - chr8_3:32057239-32057258 None:intergenic 45.0%
GCAAATCTCAGTTGCAAACG+TGG + chr8_3:32057287-32057306 Msa1285810:intron 45.0%
GCTTTGCATTGCTACATGTG+TGG + chr8_3:32057325-32057344 Msa1285810:intron 45.0%
CATTGTAGCCGATAGTACTG+GGG - chr8_3:32057375-32057394 None:intergenic 45.0%
CACATTGTAGCCGATAGTAC+TGG - chr8_3:32057377-32057396 None:intergenic 45.0%
!! TGTTGTCAATAGTGGGCTAG+TGG - chr8_3:32057400-32057419 None:intergenic 45.0%
!! GGTTTGGTGCAGAAGATTAG+TGG + chr8_3:32057780-32057799 Msa1285810:intron 45.0%
ATCACCTCAACTATCTCTGC+AGG + chr8_3:32057814-32057833 Msa1285810:intron 45.0%
! GTTTGGTTCAAACCAGGGTA+TGG + chr8_3:32058008-32058027 Msa1285810:intron 45.0%
GTTCAAACCAGGGTATGGTA+GGG + chr8_3:32058013-32058032 Msa1285810:intron 45.0%
TTCAAACCAGGGTATGGTAG+GGG + chr8_3:32058014-32058033 Msa1285810:intron 45.0%
AGGGGAGCAAAATTTCTGCT+AGG + chr8_3:32058224-32058243 Msa1285810:intron 45.0%
GGAGCAAAATTTCTGCTAGG+AGG + chr8_3:32058227-32058246 Msa1285810:intron 45.0%
CTGCTAGGAGGATATTGCTT+CGG + chr8_3:32058239-32058258 Msa1285810:intron 45.0%
TGCAAGCAAAACAGGAGTAG+GGG - chr8_3:32058268-32058287 None:intergenic 45.0%
GGATCAGGTACATTCTGCAA+TGG - chr8_3:32058509-32058528 None:intergenic 45.0%
GCTCTCTTTCTCTTCTTGTC+AGG + chr8_3:32058559-32058578 Msa1285810:intron 45.0%
CGATCTCCCACATACAGAAT+GGG - chr8_3:32058763-32058782 None:intergenic 45.0%
CCATTCTGTATGTGGGAGAT+CGG + chr8_3:32058761-32058780 Msa1285810:three_prime_UTR 45.0%
CTATGTTGGGCTCAGTATGT+TGG + chr8_3:32058856-32058875 Msa1285810:three_prime_UTR 45.0%
! ACGACTGGTTCACAGTAGAT+GGG + chr8_3:32057087-32057106 Msa1285810:CDS 45.0%
CAGTAGATGGGAAAGTGAAG+AGG + chr8_3:32057099-32057118 Msa1285810:CDS 45.0%
AGGACCGTTATTGTGTTAGG+GGG - chr8_3:32057238-32057257 None:intergenic 45.0%
CAGGACCGTTATTGTGTTAG+GGG - chr8_3:32057239-32057258 None:intergenic 45.0%
GCAAATCTCAGTTGCAAACG+TGG + chr8_3:32057287-32057306 Msa1285810:intron 45.0%
GCTTTGCATTGCTACATGTG+TGG + chr8_3:32057325-32057344 Msa1285810:intron 45.0%
CATTGTAGCCGATAGTACTG+GGG - chr8_3:32057375-32057394 None:intergenic 45.0%
CACATTGTAGCCGATAGTAC+TGG - chr8_3:32057377-32057396 None:intergenic 45.0%
!! TGTTGTCAATAGTGGGCTAG+TGG - chr8_3:32057400-32057419 None:intergenic 45.0%
!! GGTTTGGTGCAGAAGATTAG+TGG + chr8_3:32057780-32057799 Msa1285810:intron 45.0%
ATCACCTCAACTATCTCTGC+AGG + chr8_3:32057814-32057833 Msa1285810:intron 45.0%
! GTTTGGTTCAAACCAGGGTA+TGG + chr8_3:32058008-32058027 Msa1285810:intron 45.0%
GTTCAAACCAGGGTATGGTA+GGG + chr8_3:32058013-32058032 Msa1285810:intron 45.0%
TTCAAACCAGGGTATGGTAG+GGG + chr8_3:32058014-32058033 Msa1285810:intron 45.0%
AGGGGAGCAAAATTTCTGCT+AGG + chr8_3:32058224-32058243 Msa1285810:intron 45.0%
GGAGCAAAATTTCTGCTAGG+AGG + chr8_3:32058227-32058246 Msa1285810:intron 45.0%
CTGCTAGGAGGATATTGCTT+CGG + chr8_3:32058239-32058258 Msa1285810:intron 45.0%
TGCAAGCAAAACAGGAGTAG+GGG - chr8_3:32058268-32058287 None:intergenic 45.0%
GGATCAGGTACATTCTGCAA+TGG - chr8_3:32058509-32058528 None:intergenic 45.0%
GCTCTCTTTCTCTTCTTGTC+AGG + chr8_3:32058559-32058578 Msa1285810:intron 45.0%
CGATCTCCCACATACAGAAT+GGG - chr8_3:32058763-32058782 None:intergenic 45.0%
CCATTCTGTATGTGGGAGAT+CGG + chr8_3:32058761-32058780 Msa1285810:three_prime_UTR 45.0%
CTATGTTGGGCTCAGTATGT+TGG + chr8_3:32058856-32058875 Msa1285810:three_prime_UTR 45.0%
TACTGTGAACCAGTCGTGGA+CGG - chr8_3:32057084-32057103 None:intergenic 50.0%
CATCTACTGTGAACCAGTCG+TGG - chr8_3:32057088-32057107 None:intergenic 50.0%
! CACGACTGGTTCACAGTAGA+TGG + chr8_3:32057086-32057105 Msa1285810:CDS 50.0%
GGACCGTTATTGTGTTAGGG+GGG - chr8_3:32057237-32057256 None:intergenic 50.0%
TGCATTGCTACATGTGTGGG+TGG + chr8_3:32057329-32057348 Msa1285810:intron 50.0%
CACACCTGCAGAGATAGTTG+AGG - chr8_3:32057821-32057840 None:intergenic 50.0%
GGTTCAAACCAGGGTATGGT+AGG + chr8_3:32058012-32058031 Msa1285810:intron 50.0%
CCGATCTCCCACATACAGAA+TGG - chr8_3:32058764-32058783 None:intergenic 50.0%
TACTGTGAACCAGTCGTGGA+CGG - chr8_3:32057084-32057103 None:intergenic 50.0%
CATCTACTGTGAACCAGTCG+TGG - chr8_3:32057088-32057107 None:intergenic 50.0%
! CACGACTGGTTCACAGTAGA+TGG + chr8_3:32057086-32057105 Msa1285810:CDS 50.0%
GGACCGTTATTGTGTTAGGG+GGG - chr8_3:32057237-32057256 None:intergenic 50.0%
TGCATTGCTACATGTGTGGG+TGG + chr8_3:32057329-32057348 Msa1285810:intron 50.0%
CACACCTGCAGAGATAGTTG+AGG - chr8_3:32057821-32057840 None:intergenic 50.0%
GGTTCAAACCAGGGTATGGT+AGG + chr8_3:32058012-32058031 Msa1285810:intron 50.0%
CCGATCTCCCACATACAGAA+TGG - chr8_3:32058764-32058783 None:intergenic 50.0%
! TGTAGCCGATAGTACTGGGG+AGG - chr8_3:32057372-32057391 None:intergenic 55.0%
AACCAGGGTATGGTAGGGGA+TGG + chr8_3:32058018-32058037 Msa1285810:intron 55.0%
ATCCATCCCCTACCATACCC+TGG - chr8_3:32058023-32058042 None:intergenic 55.0%
GGGTATGGTAGGGGATGGAT+TGG + chr8_3:32058023-32058042 Msa1285810:intron 55.0%
! GGGCTCAGTATGTTGGTGCT+AGG + chr8_3:32058863-32058882 Msa1285810:three_prime_UTR 55.0%
! TGTAGCCGATAGTACTGGGG+AGG - chr8_3:32057372-32057391 None:intergenic 55.0%
AACCAGGGTATGGTAGGGGA+TGG + chr8_3:32058018-32058037 Msa1285810:intron 55.0%
ATCCATCCCCTACCATACCC+TGG - chr8_3:32058023-32058042 None:intergenic 55.0%
GGGTATGGTAGGGGATGGAT+TGG + chr8_3:32058023-32058042 Msa1285810:intron 55.0%
! GGGCTCAGTATGTTGGTGCT+AGG + chr8_3:32058863-32058882 Msa1285810:three_prime_UTR 55.0%
! CCGATAGTACTGGGGAGGCG+CGG - chr8_3:32057367-32057386 None:intergenic 65.0%
CCGCGCCTCCCCAGTACTAT+CGG + chr8_3:32057364-32057383 Msa1285810:intron 65.0%
! CCGATAGTACTGGGGAGGCG+CGG - chr8_3:32057367-32057386 None:intergenic 65.0%
CCGCGCCTCCCCAGTACTAT+CGG + chr8_3:32057364-32057383 Msa1285810:intron 65.0%
GAGGCTTGGCCGTCCACGAC+TGG + chr8_3:32057072-32057091 Msa1285810:CDS 70.0%
! ACTGGGGAGGCGCGGAGCAA+TGG - chr8_3:32057359-32057378 None:intergenic 70.0%
GAGGCTTGGCCGTCCACGAC+TGG + chr8_3:32057072-32057091 Msa1285810:CDS 70.0%
! ACTGGGGAGGCGCGGAGCAA+TGG - chr8_3:32057359-32057378 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 32057049 32058909 32057049 ID=Msa1285810;Name=Msa1285810
chr8_3 mRNA 32057049 32058909 32057049 ID=Msa1285810-mRNA-1;Parent=Msa1285810;Name=Msa1285810-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0.5|0.5|1|1|2|231|59
chr8_3 exon 32057049 32057130 32057049 ID=Msa1285810-mRNA-1:exon:9985;Parent=Msa1285810-mRNA-1
chr8_3 exon 32058581 32058909 32058581 ID=Msa1285810-mRNA-1:exon:9986;Parent=Msa1285810-mRNA-1
chr8_3 CDS 32057049 32057130 32057049 ID=Msa1285810-mRNA-1:cds;Parent=Msa1285810-mRNA-1
chr8_3 CDS 32058581 32058678 32058581 ID=Msa1285810-mRNA-1:cds;Parent=Msa1285810-mRNA-1
chr8_3 three_prime_UTR 32058679 32058909 32058679 ID=Msa1285810-mRNA-1:three_prime_utr;Parent=Msa1285810-mRNA-1
Gene Sequence

>Msa1285810

ATGGCTCTTGGAATGAGAATAAAGAGGCTTGGCCGTCCACGACTGGTTCACAGTAGATGGGAAAGTGAAGAGGAAAGAAGAAGTTGCACTTTAGAGCATTTTCCTGGATGTCATAACTCTGGAAAGATTATGGATGGTTTTGTATGGCTTGATGATTTTGGTTCCTTTAGGCTGTTGTAA
Protein sequence

>Msa1285810

MALGMRIKRLGRPRLVHSRWESEEERRSCTLEHFPGCHNSGKIMDGFVWLDDFGSFRLL*