AlfalfaGEDB | Msa0651050

M. sativa cultivar zhongmu-4 / Msa0651050

KEGG: K08511
GO: GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0031410 GO:0031982 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048284 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0097708 GO:0098796
PFAM: PF00957.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0651050	AVR48490.1	95.652	46	2	0	9	54	175	220	2.60e-20	89.7

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001450	Msa0651050	0.822350	2.483183e-53	-8.615850e-47
Msa0037250	Msa0651050	0.842194	3.001196e-58	-8.615850e-47
Msa0046740	Msa0651050	0.846332	2.320845e-59	-8.615850e-47
Msa0048150	Msa0651050	0.818160	2.266303e-52	-8.615850e-47
Msa0054230	Msa0651050	0.842065	3.245538e-58	-8.615850e-47
Msa0056430	Msa0651050	0.812133	4.941652e-51	-8.615850e-47
Msa0056470	Msa0651050	0.834729	2.532657e-56	-8.615850e-47
Msa0064350	Msa0651050	0.816628	5.013553e-52	-8.615850e-47
Msa0064380	Msa0651050	0.830488	2.854487e-55	-8.615850e-47
Msa0072420	Msa0651050	0.817428	3.314968e-52	-8.615850e-47
Msa0115990	Msa0651050	0.858178	9.848594e-63	-8.615850e-47
Msa0613920	Msa0651050	0.805052	1.608338e-49	-8.615850e-47
Msa0615990	Msa0651050	0.843692	1.198723e-58	-8.615850e-47
Msa0651050	Msa0661300	0.815094	1.102208e-51	-8.615850e-47
Msa0651050	Msa0666270	0.844825	5.947749e-59	-8.615850e-47
Msa0651050	Msa0690770	0.805525	1.280129e-49	-8.615850e-47
Msa0651050	Msa0698780	0.806651	7.418245e-50	-8.615850e-47
Msa0651050	Msa0784200	0.814951	1.185734e-51	-8.615850e-47
Msa0651050	Msa0818310	0.812690	3.734750e-51	-8.615850e-47
Msa0651050	Msa0820650	0.804250	2.363838e-49	-8.615850e-47
Msa0651050	Msa0858770	0.820366	7.124228e-53	-8.615850e-47
Msa0651050	Msa1078270	0.875920	2.106546e-68	-8.615850e-47
Msa0651050	Msa1081990	0.802204	6.268744e-49	-8.615850e-47
Msa0651050	Msa1123500	0.865557	5.417750e-65	-8.615850e-47
Msa0651050	Msa1130980	0.850220	1.954842e-60	-8.615850e-47
Msa0651050	Msa1165230	0.808549	2.933097e-50	-8.615850e-47
Msa0651050	Msa1165940	0.853217	2.765813e-61	-8.615850e-47
Msa0651050	Msa1169770	0.874374	7.098628e-68	-8.615850e-47
Msa0651050	Msa1239910	0.843120	1.703910e-58	-8.615850e-47
Msa0651050	Msa1244600	0.815073	1.114189e-51	-8.615850e-47
Msa0651050	Msa1269790	0.848625	5.441591e-60	-8.615850e-47
Msa0651050	Msa1285810	0.803052	4.190075e-49	-8.615850e-47
Msa0651050	Msa1341060	0.800284	1.549020e-48	-8.615850e-47
Msa0651050	Msa1343040	0.834601	2.728230e-56	-8.615850e-47
Msa0651050	Msa1357620	0.805486	1.304495e-49	-8.615850e-47
Msa0651050	Msa1413260	0.846808	1.720549e-59	-8.615850e-47
Msa0651050	Msa1418340	0.850611	1.518488e-60	-8.615850e-47
Msa0651050	Msa1425670	0.830590	2.695038e-55	-8.615850e-47
Msa0651050	Msa1428520	0.827568	1.456231e-54	-8.615850e-47
Msa0651050	Msa1451560	0.835079	2.067933e-56	-8.615850e-47
Msa0651050	Msa1456050	0.842815	2.053847e-58	-8.615850e-47
Msa0123560	Msa0651050	0.828762	7.508337e-55	-8.615850e-47
Msa0125450	Msa0651050	0.810602	1.062573e-50	-8.615850e-47
Msa0125540	Msa0651050	0.836190	1.081998e-56	-8.615850e-47
Msa0140650	Msa0651050	0.801360	9.341269e-49	-8.615850e-47
Msa0140820	Msa0651050	0.816098	6.588673e-52	-8.615850e-47
Msa0149190	Msa0651050	0.867440	1.366878e-65	-8.615850e-47
Msa0149200	Msa0651050	0.844850	5.855441e-59	-8.615850e-47
Msa0149230	Msa0651050	0.816919	4.314083e-52	-8.615850e-47
Msa0369450	Msa0651050	0.805627	1.218555e-49	-8.615850e-47
Msa0378490	Msa0651050	0.811246	7.707102e-51	-8.615850e-47
Msa0380300	Msa0651050	0.814487	1.502628e-51	-8.615850e-47
Msa0385250	Msa0651050	0.822585	2.189192e-53	-8.615850e-47
Msa0390030	Msa0651050	0.858106	1.034487e-62	-8.615850e-47
Msa0425470	Msa0651050	0.819309	1.242425e-52	-8.615850e-47
Msa0427150	Msa0651050	0.803442	3.480010e-49	-8.615850e-47
Msa0432210	Msa0651050	0.844966	5.448741e-59	-8.615850e-47
Msa0471440	Msa0651050	0.816573	5.159318e-52	-8.615850e-47
Msa0267970	Msa0651050	0.820078	8.295067e-53	-8.615850e-47
Msa0272300	Msa0651050	0.814219	1.722441e-51	-8.615850e-47
Msa0315170	Msa0651050	0.815279	1.002750e-51	-8.615850e-47
Msa0516600	Msa0651050	0.818078	2.365008e-52	-8.615850e-47
Msa0521050	Msa0651050	0.886564	3.064439e-72	-8.615850e-47
Msa0531530	Msa0651050	0.813861	2.065630e-51	-8.615850e-47
Msa0552810	Msa0651050	0.806744	7.090832e-50	-8.615850e-47
Msa0559790	Msa0651050	0.833796	4.340878e-56	-8.615850e-47
Msa0566700	Msa0651050	0.828649	7.992628e-55	-8.615850e-47
Msa0580280	Msa0651050	0.810691	1.016316e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0651050	MtrunA17_Chr2g0296021	68.293	41	13	9	49	173	213	7.00e-14	62.4
Msa0651050	MtrunA17_Chr1g0169861	58.537	41	17	9	49	223	263	7.76e-12	57.4
Msa0651050	MtrunA17_Chr4g0043521	100.000	23	0	9	31	175	197	5.26e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 31 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAGGCAGGCACAAGACTTC+AGG	0.377030	4_3:+58329562	Msa0651050:intron
ATTGCATGAATAATGATAAC+AGG	0.397588	4_3:-58329952	None:intergenic
CTTAAAAGTGAACGGTATAC+AGG	0.429263	4_3:-58329830	None:intergenic
GAAGATAAAGCTGATTGTAT+TGG	0.457650	4_3:+58329629	Msa0651050:CDS
GATGGCTCTCCCGAGTGTTA+TGG	0.457911	4_3:+58329425	Msa0651050:CDS
TATTTATTCTTAAGAACGAT+TGG	0.463535	4_3:-58329687	None:intergenic
GAATGATTTCTCGCAAGAAA+AGG	0.473434	4_3:+58330004	Msa0651050:three_prime_UTR
TGATGATGAGGAAAGATATC+AGG	0.485489	4_3:+58329769	Msa0651050:three_prime_UTR
ATTTATTCTTAAGAACGATT+GGG	0.491549	4_3:-58329686	None:intergenic
ACTTGATGTAGGTCCTGGAC+AGG	0.498294	4_3:+58329242	Msa0651050:five_prime_UTR
AATTACTAACCTGGTGATGA+AGG	0.503755	4_3:-58329303	None:intergenic
TTGAAACTTGATGTAGGTCC+TGG	0.503906	4_3:+58329237	Msa0651050:five_prime_UTR
GCACAAGACTTCAGGAACTC+AGG	0.526353	4_3:+58329570	Msa0651050:CDS
CTTGATGTAGGTCCTGGACA+GGG	0.526403	4_3:+58329243	Msa0651050:five_prime_UTR
CGATTGGGAGGACAATTATG+AGG	0.534617	4_3:-58329671	None:intergenic
GAAATCAAACTACAATAGAC+AGG	0.545244	4_3:-58329907	None:intergenic
GAATAAATAAACGCCACTCT+CGG	0.554345	4_3:+58329701	Msa0651050:exon
ACATGTTTGAAACTTGATGT+AGG	0.559859	4_3:+58329231	Msa0651050:five_prime_UTR
TGATGTAGGTCCTGGACAGG+GGG	0.582640	4_3:+58329245	Msa0651050:five_prime_UTR
CACAAGACTTCAGGAACTCA+GGG	0.588787	4_3:+58329571	Msa0651050:CDS
AAGATAAATGTCTCCGAGAG+TGG	0.592683	4_3:-58329714	None:intergenic
GAGATCTTTACCATAACACT+CGG	0.595500	4_3:-58329435	None:intergenic
GACTGAGAACCTTCATCACC+AGG	0.596496	4_3:+58329294	Msa0651050:five_prime_UTR
TTGATGTAGGTCCTGGACAG+GGG	0.598139	4_3:+58329244	Msa0651050:five_prime_UTR
TATTCTTAAGAACGATTGGG+AGG	0.607627	4_3:-58329683	None:intergenic
CATACACAAGGTGACAAACA+AGG	0.607905	4_3:-58329982	None:intergenic
CGAGAAATCATTCATACACA+AGG	0.626938	4_3:-58329994	None:intergenic
ATTATTATATGATGATGATG+AGG	0.648084	4_3:+58329757	Msa0651050:three_prime_UTR
GATGTAGGTCCTGGACAGGG+GGG	0.679259	4_3:+58329246	Msa0651050:five_prime_UTR
AGATCTTTACCATAACACTC+GGG	0.700225	4_3:-58329434	None:intergenic
ATAATGATAACAGGATGTGT+CGG	0.710316	4_3:-58329943	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CATTTTTTTATGATAAATGA+TGG	+	chr4_3:58329407-58329426	Msa0651050:exon	15.0%
!!!	CATTTTTTTATGATAAATGA+TGG	+	chr4_3:58329407-58329426	Msa0651050:exon	15.0%
!!	ATTTATTCTTAAGAACGATT+GGG	-	chr4_3:58329689-58329708	None:intergenic	20.0%
!!	TATTTATTCTTAAGAACGAT+TGG	-	chr4_3:58329690-58329709	None:intergenic	20.0%
!!!	ATTATTATATGATGATGATG+AGG	+	chr4_3:58329757-58329776	Msa0651050:three_prime_UTR	20.0%
!!	TGTAAAAACTTAAAAGTGAA+CGG	-	chr4_3:58329841-58329860	None:intergenic	20.0%
!!!	TTTAAGTTTTTACAGTATGA+TGG	+	chr4_3:58329847-58329866	Msa0651050:three_prime_UTR	20.0%
!!	ATTTATTCTTAAGAACGATT+GGG	-	chr4_3:58329689-58329708	None:intergenic	20.0%
!!	TATTTATTCTTAAGAACGAT+TGG	-	chr4_3:58329690-58329709	None:intergenic	20.0%
!!!	ATTATTATATGATGATGATG+AGG	+	chr4_3:58329757-58329776	Msa0651050:three_prime_UTR	20.0%
!!	TGTAAAAACTTAAAAGTGAA+CGG	-	chr4_3:58329841-58329860	None:intergenic	20.0%
!!!	TTTAAGTTTTTACAGTATGA+TGG	+	chr4_3:58329847-58329866	Msa0651050:three_prime_UTR	20.0%
!!	TCGATTTGAAGAAAAAAAAC+AGG	-	chr4_3:58329518-58329537	None:intergenic	25.0%
!	ATTGCATGAATAATGATAAC+AGG	-	chr4_3:58329955-58329974	None:intergenic	25.0%
!!!	CAAGAAAAGGTTTTATTATC+TGG	+	chr4_3:58330017-58330036	Msa0651050:three_prime_UTR	25.0%
!!	TCGATTTGAAGAAAAAAAAC+AGG	-	chr4_3:58329518-58329537	None:intergenic	25.0%
!	ATTGCATGAATAATGATAAC+AGG	-	chr4_3:58329955-58329974	None:intergenic	25.0%
!	ACATGTTTGAAACTTGATGT+AGG	+	chr4_3:58329231-58329250	Msa0651050:five_prime_UTR	30.0%
	TTAGTAATTGTGTGATTGGA+AGG	+	chr4_3:58329317-58329336	Msa0651050:intron	30.0%
	TGAAGAAAAAAAACAGGCAA+TGG	-	chr4_3:58329512-58329531	None:intergenic	30.0%
!!!	TTCTAACTTGCTGTTTTATG+AGG	+	chr4_3:58329544-58329563	Msa0651050:intron	30.0%
	GAAGATAAAGCTGATTGTAT+TGG	+	chr4_3:58329629-58329648	Msa0651050:CDS	30.0%
	GAAATCAAACTACAATAGAC+AGG	-	chr4_3:58329910-58329929	None:intergenic	30.0%
	ATAATGATAACAGGATGTGT+CGG	-	chr4_3:58329946-58329965	None:intergenic	30.0%
!	ACATGTTTGAAACTTGATGT+AGG	+	chr4_3:58329231-58329250	Msa0651050:five_prime_UTR	30.0%
	TTAGTAATTGTGTGATTGGA+AGG	+	chr4_3:58329317-58329336	Msa0651050:intron	30.0%
	TGAAGAAAAAAAACAGGCAA+TGG	-	chr4_3:58329512-58329531	None:intergenic	30.0%
!!!	TTCTAACTTGCTGTTTTATG+AGG	+	chr4_3:58329544-58329563	Msa0651050:intron	30.0%
	GAAGATAAAGCTGATTGTAT+TGG	+	chr4_3:58329629-58329648	Msa0651050:CDS	30.0%
	GAAATCAAACTACAATAGAC+AGG	-	chr4_3:58329910-58329929	None:intergenic	30.0%
	ATAATGATAACAGGATGTGT+CGG	-	chr4_3:58329946-58329965	None:intergenic	30.0%
!	AATTACTAACCTGGTGATGA+AGG	-	chr4_3:58329306-58329325	None:intergenic	35.0%
	CAATCACACAATTACTAACC+TGG	-	chr4_3:58329315-58329334	None:intergenic	35.0%
	CAGGTTAGTAATTGTGTGAT+TGG	+	chr4_3:58329313-58329332	Msa0651050:intron	35.0%
	AGATCTTTACCATAACACTC+GGG	-	chr4_3:58329437-58329456	None:intergenic	35.0%
	GAGATCTTTACCATAACACT+CGG	-	chr4_3:58329438-58329457	None:intergenic	35.0%
	TATTCTTAAGAACGATTGGG+AGG	-	chr4_3:58329686-58329705	None:intergenic	35.0%
	GAATAAATAAACGCCACTCT+CGG	+	chr4_3:58329701-58329720	Msa0651050:exon	35.0%
!	TGATGATGAGGAAAGATATC+AGG	+	chr4_3:58329769-58329788	Msa0651050:three_prime_UTR	35.0%
	CTTAAAAGTGAACGGTATAC+AGG	-	chr4_3:58329833-58329852	None:intergenic	35.0%
	CGAGAAATCATTCATACACA+AGG	-	chr4_3:58329997-58330016	None:intergenic	35.0%
	GAATGATTTCTCGCAAGAAA+AGG	+	chr4_3:58330004-58330023	Msa0651050:three_prime_UTR	35.0%
!	AATTACTAACCTGGTGATGA+AGG	-	chr4_3:58329306-58329325	None:intergenic	35.0%
	CAATCACACAATTACTAACC+TGG	-	chr4_3:58329315-58329334	None:intergenic	35.0%
	CAGGTTAGTAATTGTGTGAT+TGG	+	chr4_3:58329313-58329332	Msa0651050:intron	35.0%
	AGATCTTTACCATAACACTC+GGG	-	chr4_3:58329437-58329456	None:intergenic	35.0%
	GAGATCTTTACCATAACACT+CGG	-	chr4_3:58329438-58329457	None:intergenic	35.0%
	TATTCTTAAGAACGATTGGG+AGG	-	chr4_3:58329686-58329705	None:intergenic	35.0%
	GAATAAATAAACGCCACTCT+CGG	+	chr4_3:58329701-58329720	Msa0651050:exon	35.0%
!	TGATGATGAGGAAAGATATC+AGG	+	chr4_3:58329769-58329788	Msa0651050:three_prime_UTR	35.0%
	CTTAAAAGTGAACGGTATAC+AGG	-	chr4_3:58329833-58329852	None:intergenic	35.0%
	CGAGAAATCATTCATACACA+AGG	-	chr4_3:58329997-58330016	None:intergenic	35.0%
	GAATGATTTCTCGCAAGAAA+AGG	+	chr4_3:58330004-58330023	Msa0651050:three_prime_UTR	35.0%
	TTGAAACTTGATGTAGGTCC+TGG	+	chr4_3:58329237-58329256	Msa0651050:five_prime_UTR	40.0%
	ATCCCACACAAAAGATGAGT+CGG	+	chr4_3:58329471-58329490	Msa0651050:intron	40.0%
!!!	AACTTGCTGTTTTATGAGGC+AGG	+	chr4_3:58329548-58329567	Msa0651050:intron	40.0%
!	CCACATTTTTCTGCGGATTT+TGG	-	chr4_3:58329598-58329617	None:intergenic	40.0%
	CCAAAATCCGCAGAAAAATG+TGG	+	chr4_3:58329595-58329614	Msa0651050:CDS	40.0%
!	TTTGCAGCCACATTTTTCTG+CGG	-	chr4_3:58329605-58329624	None:intergenic	40.0%
	AAGATAAATGTCTCCGAGAG+TGG	-	chr4_3:58329717-58329736	None:intergenic	40.0%
	CATACACAAGGTGACAAACA+AGG	-	chr4_3:58329985-58330004	None:intergenic	40.0%
	TTGAAACTTGATGTAGGTCC+TGG	+	chr4_3:58329237-58329256	Msa0651050:five_prime_UTR	40.0%
	ATCCCACACAAAAGATGAGT+CGG	+	chr4_3:58329471-58329490	Msa0651050:intron	40.0%
!!!	AACTTGCTGTTTTATGAGGC+AGG	+	chr4_3:58329548-58329567	Msa0651050:intron	40.0%
!	CCACATTTTTCTGCGGATTT+TGG	-	chr4_3:58329598-58329617	None:intergenic	40.0%
	CCAAAATCCGCAGAAAAATG+TGG	+	chr4_3:58329595-58329614	Msa0651050:CDS	40.0%
!	TTTGCAGCCACATTTTTCTG+CGG	-	chr4_3:58329605-58329624	None:intergenic	40.0%
	AAGATAAATGTCTCCGAGAG+TGG	-	chr4_3:58329717-58329736	None:intergenic	40.0%
	CATACACAAGGTGACAAACA+AGG	-	chr4_3:58329985-58330004	None:intergenic	40.0%
!	GGGGGAAAAGATAGAGCTTT+TGG	+	chr4_3:58329264-58329283	Msa0651050:five_prime_UTR	45.0%
!	ATCTTTTGTGTGGGATGGAG+AGG	-	chr4_3:58329467-58329486	None:intergenic	45.0%
!	GACTCATCTTTTGTGTGGGA+TGG	-	chr4_3:58329472-58329491	None:intergenic	45.0%
	TCCCACACAAAAGATGAGTC+GGG	+	chr4_3:58329472-58329491	Msa0651050:intron	45.0%
	CACAAGACTTCAGGAACTCA+GGG	+	chr4_3:58329571-58329590	Msa0651050:CDS	45.0%
	CGATTGGGAGGACAATTATG+AGG	-	chr4_3:58329674-58329693	None:intergenic	45.0%
!	GGGGGAAAAGATAGAGCTTT+TGG	+	chr4_3:58329264-58329283	Msa0651050:five_prime_UTR	45.0%
!	ATCTTTTGTGTGGGATGGAG+AGG	-	chr4_3:58329467-58329486	None:intergenic	45.0%
!	GACTCATCTTTTGTGTGGGA+TGG	-	chr4_3:58329472-58329491	None:intergenic	45.0%
	TCCCACACAAAAGATGAGTC+GGG	+	chr4_3:58329472-58329491	Msa0651050:intron	45.0%
	CACAAGACTTCAGGAACTCA+GGG	+	chr4_3:58329571-58329590	Msa0651050:CDS	45.0%
	CGATTGGGAGGACAATTATG+AGG	-	chr4_3:58329674-58329693	None:intergenic	45.0%
	ACTTGATGTAGGTCCTGGAC+AGG	+	chr4_3:58329242-58329261	Msa0651050:five_prime_UTR	50.0%
	CTTGATGTAGGTCCTGGACA+GGG	+	chr4_3:58329243-58329262	Msa0651050:five_prime_UTR	50.0%
	TTGATGTAGGTCCTGGACAG+GGG	+	chr4_3:58329244-58329263	Msa0651050:five_prime_UTR	50.0%
	GACTGAGAACCTTCATCACC+AGG	+	chr4_3:58329294-58329313	Msa0651050:five_prime_UTR	50.0%
	TTGTGTGGGATGGAGAGGAA+GGG	-	chr4_3:58329462-58329481	None:intergenic	50.0%
	TTTGTGTGGGATGGAGAGGA+AGG	-	chr4_3:58329463-58329482	None:intergenic	50.0%
!	CCCCGACTCATCTTTTGTGT+GGG	-	chr4_3:58329476-58329495	None:intergenic	50.0%
	CCCACACAAAAGATGAGTCG+GGG	+	chr4_3:58329473-58329492	Msa0651050:intron	50.0%
	CCACACAAAAGATGAGTCGG+GGG	+	chr4_3:58329474-58329493	Msa0651050:intron	50.0%
	GCACAAGACTTCAGGAACTC+AGG	+	chr4_3:58329570-58329589	Msa0651050:CDS	50.0%
	ACTTGATGTAGGTCCTGGAC+AGG	+	chr4_3:58329242-58329261	Msa0651050:five_prime_UTR	50.0%
	CTTGATGTAGGTCCTGGACA+GGG	+	chr4_3:58329243-58329262	Msa0651050:five_prime_UTR	50.0%
	TTGATGTAGGTCCTGGACAG+GGG	+	chr4_3:58329244-58329263	Msa0651050:five_prime_UTR	50.0%
	GACTGAGAACCTTCATCACC+AGG	+	chr4_3:58329294-58329313	Msa0651050:five_prime_UTR	50.0%
	TTGTGTGGGATGGAGAGGAA+GGG	-	chr4_3:58329462-58329481	None:intergenic	50.0%
	TTTGTGTGGGATGGAGAGGA+AGG	-	chr4_3:58329463-58329482	None:intergenic	50.0%
!	CCCCGACTCATCTTTTGTGT+GGG	-	chr4_3:58329476-58329495	None:intergenic	50.0%
	CCCACACAAAAGATGAGTCG+GGG	+	chr4_3:58329473-58329492	Msa0651050:intron	50.0%
	CCACACAAAAGATGAGTCGG+GGG	+	chr4_3:58329474-58329493	Msa0651050:intron	50.0%
	GCACAAGACTTCAGGAACTC+AGG	+	chr4_3:58329570-58329589	Msa0651050:CDS	50.0%
	TGATGTAGGTCCTGGACAGG+GGG	+	chr4_3:58329245-58329264	Msa0651050:five_prime_UTR	55.0%
!	CTATCTTTTCCCCCCTGTCC+AGG	-	chr4_3:58329258-58329277	None:intergenic	55.0%
	GATGGCTCTCCCGAGTGTTA+TGG	+	chr4_3:58329425-58329444	Msa0651050:CDS	55.0%
	TGTGTGGGATGGAGAGGAAG+GGG	-	chr4_3:58329461-58329480	None:intergenic	55.0%
!	CCCCCGACTCATCTTTTGTG+TGG	-	chr4_3:58329477-58329496	None:intergenic	55.0%
	TGAGGCAGGCACAAGACTTC+AGG	+	chr4_3:58329562-58329581	Msa0651050:intron	55.0%
	TGATGTAGGTCCTGGACAGG+GGG	+	chr4_3:58329245-58329264	Msa0651050:five_prime_UTR	55.0%
!	CTATCTTTTCCCCCCTGTCC+AGG	-	chr4_3:58329258-58329277	None:intergenic	55.0%
	GATGGCTCTCCCGAGTGTTA+TGG	+	chr4_3:58329425-58329444	Msa0651050:CDS	55.0%
	TGTGTGGGATGGAGAGGAAG+GGG	-	chr4_3:58329461-58329480	None:intergenic	55.0%
!	CCCCCGACTCATCTTTTGTG+TGG	-	chr4_3:58329477-58329496	None:intergenic	55.0%
	TGAGGCAGGCACAAGACTTC+AGG	+	chr4_3:58329562-58329581	Msa0651050:intron	55.0%
	GATGTAGGTCCTGGACAGGG+GGG	+	chr4_3:58329246-58329265	Msa0651050:five_prime_UTR	60.0%
	GTGTGGGATGGAGAGGAAGG+GGG	-	chr4_3:58329460-58329479	None:intergenic	60.0%
	GATGTAGGTCCTGGACAGGG+GGG	+	chr4_3:58329246-58329265	Msa0651050:five_prime_UTR	60.0%
	GTGTGGGATGGAGAGGAAGG+GGG	-	chr4_3:58329460-58329479	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	58329229	58330039	58329229	ID=Msa0651050;Name=Msa0651050
chr4_3	mRNA	58329229	58330039	58329229	ID=Msa0651050-mRNA-1;Parent=Msa0651050;Name=Msa0651050-mRNA-1;_AED=0.04;_eAED=0.04;_QI=112\|0\|0.66\|0.66\|1\|1\|3\|329\|54
chr4_3	exon	58329229	58329315	58329229	ID=Msa0651050-mRNA-1:exon:15409;Parent=Msa0651050-mRNA-1
chr4_3	exon	58329398	58329446	58329398	ID=Msa0651050-mRNA-1:exon:15410;Parent=Msa0651050-mRNA-1
chr4_3	exon	58329570	58330039	58329570	ID=Msa0651050-mRNA-1:exon:15411;Parent=Msa0651050-mRNA-1
chr4_3	five_prime_UTR	58329229	58329315	58329229	ID=Msa0651050-mRNA-1:five_prime_utr;Parent=Msa0651050-mRNA-1
chr4_3	five_prime_UTR	58329398	58329422	58329398	ID=Msa0651050-mRNA-1:five_prime_utr;Parent=Msa0651050-mRNA-1
chr4_3	CDS	58329423	58329446	58329423	ID=Msa0651050-mRNA-1:cds;Parent=Msa0651050-mRNA-1
chr4_3	CDS	58329570	58329710	58329570	ID=Msa0651050-mRNA-1:cds;Parent=Msa0651050-mRNA-1
chr4_3	three_prime_UTR	58329711	58330039	58329711	ID=Msa0651050-mRNA-1:three_prime_utr;Parent=Msa0651050-mRNA-1

Gene Sequence	>Msa0651050 ATGATGGCTCTCCCGAGTGTTATGGCACAAGACTTCAGGAACTCAGGGACCAAAATCCGCAGAAAAATGTGGCTGCAAAATATGAAGATAAAGCTGATTGTATTGGCAATTTTGATAGCTCTGATCCTCATAATTGTCCTCCCAATCGTTCTTAAGAATAAATAA
Protein sequence	>Msa0651050 MMALPSVMAQDFRNSGTKIRRKMWLQNMKIKLIVLAILIALILIIVLPIVLKNK*