AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0054230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054230 MtrunA17_Chr8g0347021 100.000 41 0 0 1 41 106 146 7.13e-24 85.9
Msa0054230 MtrunA17_Chr8g0347341 100.000 41 0 0 1 41 256 296 1.68e-22 85.5
Msa0054230 MtrunA17_Chr1g0150341 100.000 29 0 0 1 29 33 61 6.20e-15 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054230 AT5G10780.1 80.556 36 7 0 5 40 144 179 7.48e-15 63.9

Find 40 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTCTTGGAGGAATTTA+AGG 0.091841 1_2:-8970175 Msa0054230:three_prime_UTR
GGTATTCATCGCCCTTAATT+TGG 0.174653 1_2:-8971394 Msa0054230:five_prime_UTR
TACTGCAAATCTGATGTAAA+AGG 0.202542 1_2:-8969932 Msa0054230:three_prime_UTR
GTAACATGCCTCCCAAATTA+AGG 0.244098 1_2:+8971382 None:intergenic
CTTGGAGGAATTTAAGGATT+TGG 0.280619 1_2:-8970169 Msa0054230:three_prime_UTR
TCTTCTGCAGCTCAATTCAT+TGG 0.332546 1_2:-8970984 Msa0054230:intron
CTTTGTATCTCTTTGTATGT+AGG 0.333936 1_2:-8969994 Msa0054230:three_prime_UTR
GTATTCATCGCCCTTAATTT+GGG 0.348550 1_2:-8971393 Msa0054230:five_prime_UTR
TACAAACCATGTCTAATCTT+TGG 0.367592 1_2:+8970056 None:intergenic
GATGCTAAAGTGGAGCTTCT+TGG 0.369452 1_2:-8971426 Msa0054230:five_prime_UTR
AATTTGGGAGGCATGTTACT+AGG 0.402445 1_2:-8971378 Msa0054230:exon
GAGGCATGTTACTAGGTGTT+TGG 0.405795 1_2:-8971371 Msa0054230:exon
ATGCTAAAGTGGAGCTTCTT+GGG 0.409851 1_2:-8971425 Msa0054230:five_prime_UTR
TCCACTTTAGCATCTTTGTA+AGG 0.413449 1_2:+8971435 None:intergenic
CTTCTGCAGCTCAATTCATT+GGG 0.415950 1_2:-8970983 Msa0054230:intron
TGGTCACAAATGATCTCATT+AGG 0.422294 1_2:-8970127 Msa0054230:three_prime_UTR
TAACATGCCTCCCAAATTAA+GGG 0.464691 1_2:+8971383 None:intergenic
TTCCTACCCACACATCAGAC+TGG 0.494006 1_2:-8970955 Msa0054230:CDS
AAAGAAAATCCAACCTGAGC+AGG 0.500189 1_2:+8970917 None:intergenic
TCTTTGGTGACTGCAAGTAT+TGG 0.501455 1_2:+8970072 None:intergenic
CAGGAGGTGGAGTACTCAGG+TGG 0.509179 1_2:-8970240 Msa0054230:CDS
GTCATCCTTAGCACCTGCTC+AGG 0.516236 1_2:-8970930 Msa0054230:intron
GTACTGTTAGCATGTTGTAA+TGG 0.518335 1_2:-8970147 Msa0054230:three_prime_UTR
CTTCAGGAGGTGGAGTACTC+AGG 0.524189 1_2:-8970243 Msa0054230:intron
GAGGTGGAGTACTCAGGTGG+GGG 0.524742 1_2:-8970237 Msa0054230:CDS
GGAGCTTCTTGGGCCTAAGT+TGG 0.528352 1_2:-8971415 Msa0054230:five_prime_UTR
ACCTTACAAAGATGCTAAAG+TGG 0.529869 1_2:-8971436 Msa0054230:five_prime_UTR
AGGAGGTGGAGTACTCAGGT+GGG 0.535718 1_2:-8970239 Msa0054230:CDS
CATGTTACTAGGTGTTTGGA+AGG 0.537062 1_2:-8971367 Msa0054230:intron
TCCAACCTGAGCAGGTGCTA+AGG 0.537452 1_2:+8970925 None:intergenic
CAGTCACCAAAGATTAGACA+TGG 0.538585 1_2:-8970062 Msa0054230:three_prime_UTR
CTTAAGAACTGGAGCATCAA+CGG 0.547901 1_2:+8970205 None:intergenic
GGAGGTGGAGTACTCAGGTG+GGG 0.550312 1_2:-8970238 Msa0054230:CDS
TCCTACCCACACATCAGACT+GGG 0.552562 1_2:-8970954 Msa0054230:CDS
AGGGCGATGAATACCAACTT+AGG 0.558217 1_2:+8971402 None:intergenic
TTCATCGCCCTTAATTTGGG+AGG 0.578157 1_2:-8971390 Msa0054230:five_prime_UTR
ACATACAAAGAGATACAAAG+AGG 0.639704 1_2:+8969997 None:intergenic
TGACACCCAGTCTGATGTGT+GGG 0.677476 1_2:+8970949 None:intergenic
ATGACACCCAGTCTGATGTG+TGG 0.709294 1_2:+8970948 None:intergenic
ACCCAGTCTGATGTGTGGGT+AGG 0.740029 1_2:+8970953 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAATAATAATAAGTATA+TGG + chr1_2:8970290-8970309 None:intergenic 10.0%
!! GAAAAATAATAATAAGTATA+TGG + chr1_2:8970290-8970309 None:intergenic 10.0%
!! TTGTATGTTATTAAAATGTT+TGG - chr1_2:8970591-8970610 Msa0054230:intron 15.0%
!! TGTATGTTATTAAAATGTTT+GGG - chr1_2:8970592-8970611 Msa0054230:intron 15.0%
!! TTTGAAAATAAATCTATAAC+TGG - chr1_2:8970924-8970943 Msa0054230:intron 15.0%
!!! AAAAAAAATCTATAACTTGT+TGG - chr1_2:8970957-8970976 Msa0054230:CDS 15.0%
!! TTGTATGTTATTAAAATGTT+TGG - chr1_2:8970591-8970610 Msa0054230:intron 15.0%
!! TGTATGTTATTAAAATGTTT+GGG - chr1_2:8970592-8970611 Msa0054230:intron 15.0%
!! TTTGAAAATAAATCTATAAC+TGG - chr1_2:8970924-8970943 Msa0054230:intron 15.0%
!!! AAAAAAAATCTATAACTTGT+TGG - chr1_2:8970957-8970976 Msa0054230:CDS 15.0%
!!! TTTAACTCATTTTTGTTTCA+AGG + chr1_2:8969984-8970003 None:intergenic 20.0%
!!! TTACCTCAAATTTTAAGTTT+TGG - chr1_2:8970145-8970164 Msa0054230:three_prime_UTR 20.0%
!! TACGTTCTATAATATATTTG+AGG - chr1_2:8970200-8970219 Msa0054230:three_prime_UTR 20.0%
!!! CTATAATATATTTGAGGTTT+AGG - chr1_2:8970206-8970225 Msa0054230:exon 20.0%
!! ATTCTTAAGTTTGAAAATCT+TGG - chr1_2:8970262-8970281 Msa0054230:intron 20.0%
!! TTAATAACATACAATGCTAT+GGG + chr1_2:8970585-8970604 None:intergenic 20.0%
!! TTTAATAACATACAATGCTA+TGG + chr1_2:8970586-8970605 None:intergenic 20.0%
!!! CATTATTTCTGATATTAAAG+TGG + chr1_2:8970898-8970917 None:intergenic 20.0%
!!! AGTTATAGATTTTTTTTCAG+TGG + chr1_2:8970954-8970973 None:intergenic 20.0%
!! AATAGCATACTAAAAATGTT+AGG - chr1_2:8971407-8971426 Msa0054230:five_prime_UTR 20.0%
!! ATAGCATACTAAAAATGTTA+GGG - chr1_2:8971408-8971427 Msa0054230:five_prime_UTR 20.0%
!!! TTTAACTCATTTTTGTTTCA+AGG + chr1_2:8969984-8970003 None:intergenic 20.0%
!!! TTACCTCAAATTTTAAGTTT+TGG - chr1_2:8970145-8970164 Msa0054230:three_prime_UTR 20.0%
!! TACGTTCTATAATATATTTG+AGG - chr1_2:8970200-8970219 Msa0054230:three_prime_UTR 20.0%
!!! CTATAATATATTTGAGGTTT+AGG - chr1_2:8970206-8970225 Msa0054230:exon 20.0%
!! ATTCTTAAGTTTGAAAATCT+TGG - chr1_2:8970262-8970281 Msa0054230:intron 20.0%
!! TTAATAACATACAATGCTAT+GGG + chr1_2:8970585-8970604 None:intergenic 20.0%
!! TTTAATAACATACAATGCTA+TGG + chr1_2:8970586-8970605 None:intergenic 20.0%
!!! CATTATTTCTGATATTAAAG+TGG + chr1_2:8970898-8970917 None:intergenic 20.0%
!!! AGTTATAGATTTTTTTTCAG+TGG + chr1_2:8970954-8970973 None:intergenic 20.0%
!! AATAGCATACTAAAAATGTT+AGG - chr1_2:8971407-8971426 Msa0054230:five_prime_UTR 20.0%
!! ATAGCATACTAAAAATGTTA+GGG - chr1_2:8971408-8971427 Msa0054230:five_prime_UTR 20.0%
! ATTGATAAACTCTTGATTCT+AGG - chr1_2:8970078-8970097 Msa0054230:three_prime_UTR 25.0%
! TTGATAAACTCTTGATTCTA+GGG - chr1_2:8970079-8970098 Msa0054230:three_prime_UTR 25.0%
! ACAAAACCATGTATATACTT+AGG + chr1_2:8970125-8970144 None:intergenic 25.0%
! GAACCAAAACTTAAAATTTG+AGG + chr1_2:8970151-8970170 None:intergenic 25.0%
!!! TAAGTTTTGGTTCATTTTGT+TGG - chr1_2:8970158-8970177 Msa0054230:three_prime_UTR 25.0%
!! TTCAGAGGACTAAAATATTT+TGG + chr1_2:8970462-8970481 None:intergenic 25.0%
!!! CGTTTTAATTAATGTTTCAG+AGG + chr1_2:8970477-8970496 None:intergenic 25.0%
!! TTGCTTTATTGAGATGAAAT+TGG - chr1_2:8970686-8970705 Msa0054230:intron 25.0%
! CTTACTACAAGTAATTTCTA+TGG - chr1_2:8970731-8970750 Msa0054230:intron 25.0%
! GTGGTTCATTTATATTATTC+TGG + chr1_2:8970765-8970784 None:intergenic 25.0%
! ATAAATGAACCACAATCATT+TGG - chr1_2:8970772-8970791 Msa0054230:intron 25.0%
! ATTTATACACCAAATGATTG+TGG + chr1_2:8970784-8970803 None:intergenic 25.0%
! ACTTAAAGAAAAGAAACCAA+GGG + chr1_2:8971028-8971047 None:intergenic 25.0%
! AAGACAAAAAACTTAAGAAC+TGG + chr1_2:8971124-8971143 None:intergenic 25.0%
!!! TTAAGTTTTTTGTCTTCTCT+TGG - chr1_2:8971128-8971147 Msa0054230:intron 25.0%
! TAGCATACTAAAAATGTTAG+GGG - chr1_2:8971409-8971428 Msa0054230:five_prime_UTR 25.0%
! ATCTTTGTAAGGTTCAAATA+CGG + chr1_2:8969872-8969891 None:intergenic 25.0%
! ATTGATAAACTCTTGATTCT+AGG - chr1_2:8970078-8970097 Msa0054230:three_prime_UTR 25.0%
! TTGATAAACTCTTGATTCTA+GGG - chr1_2:8970079-8970098 Msa0054230:three_prime_UTR 25.0%
! ACAAAACCATGTATATACTT+AGG + chr1_2:8970125-8970144 None:intergenic 25.0%
! GAACCAAAACTTAAAATTTG+AGG + chr1_2:8970151-8970170 None:intergenic 25.0%
!!! TAAGTTTTGGTTCATTTTGT+TGG - chr1_2:8970158-8970177 Msa0054230:three_prime_UTR 25.0%
!! TTCAGAGGACTAAAATATTT+TGG + chr1_2:8970462-8970481 None:intergenic 25.0%
!!! CGTTTTAATTAATGTTTCAG+AGG + chr1_2:8970477-8970496 None:intergenic 25.0%
!! TTGCTTTATTGAGATGAAAT+TGG - chr1_2:8970686-8970705 Msa0054230:intron 25.0%
! CTTACTACAAGTAATTTCTA+TGG - chr1_2:8970731-8970750 Msa0054230:intron 25.0%
! GTGGTTCATTTATATTATTC+TGG + chr1_2:8970765-8970784 None:intergenic 25.0%
! ATAAATGAACCACAATCATT+TGG - chr1_2:8970772-8970791 Msa0054230:intron 25.0%
! ATTTATACACCAAATGATTG+TGG + chr1_2:8970784-8970803 None:intergenic 25.0%
! ACTTAAAGAAAAGAAACCAA+GGG + chr1_2:8971028-8971047 None:intergenic 25.0%
! AAGACAAAAAACTTAAGAAC+TGG + chr1_2:8971124-8971143 None:intergenic 25.0%
!!! TTAAGTTTTTTGTCTTCTCT+TGG - chr1_2:8971128-8971147 Msa0054230:intron 25.0%
! TAGCATACTAAAAATGTTAG+GGG - chr1_2:8971409-8971428 Msa0054230:five_prime_UTR 25.0%
TATCAGCCTAAGTATATACA+TGG - chr1_2:8970116-8970135 Msa0054230:three_prime_UTR 30.0%
! GTTCATTTTGTTGGATAGTT+GGG - chr1_2:8970167-8970186 Msa0054230:three_prime_UTR 30.0%
! ACATAATTTTACCGTCTTCA+AGG - chr1_2:8970502-8970521 Msa0054230:intron 30.0%
TTGTCATAATACCTTGAAGA+CGG + chr1_2:8970516-8970535 None:intergenic 30.0%
AATCTACCAATTCTTCACTA+TGG + chr1_2:8970820-8970839 None:intergenic 30.0%
AAATCACAAAAGGATGAAGT+AGG + chr1_2:8970990-8971009 None:intergenic 30.0%
CACTTAAAGAAAAGAAACCA+AGG + chr1_2:8971029-8971048 None:intergenic 30.0%
CCTGAAGATACAAAAAAGTA+AGG + chr1_2:8971059-8971078 None:intergenic 30.0%
!!! CCTTACTTTTTTGTATCTTC+AGG - chr1_2:8971056-8971075 Msa0054230:intron 30.0%
!!! TACTTTTTTGTATCTTCAGG+AGG - chr1_2:8971059-8971078 Msa0054230:intron 30.0%
TACAAACCATGTCTAATCTT+TGG + chr1_2:8971262-8971281 None:intergenic 30.0%
ACATACAAAGAGATACAAAG+AGG + chr1_2:8971321-8971340 None:intergenic 30.0%
CTTTGTATCTCTTTGTATGT+AGG - chr1_2:8971321-8971340 Msa0054230:intron 30.0%
TACTGCAAATCTGATGTAAA+AGG - chr1_2:8971383-8971402 Msa0054230:exon 30.0%
!!! AGGAGAATTTTTTTAGCAGT+GGG - chr1_2:8971432-8971451 Msa0054230:five_prime_UTR 30.0%
TATCAGCCTAAGTATATACA+TGG - chr1_2:8970116-8970135 Msa0054230:three_prime_UTR 30.0%
! GTTCATTTTGTTGGATAGTT+GGG - chr1_2:8970167-8970186 Msa0054230:three_prime_UTR 30.0%
! ACATAATTTTACCGTCTTCA+AGG - chr1_2:8970502-8970521 Msa0054230:intron 30.0%
TTGTCATAATACCTTGAAGA+CGG + chr1_2:8970516-8970535 None:intergenic 30.0%
AATCTACCAATTCTTCACTA+TGG + chr1_2:8970820-8970839 None:intergenic 30.0%
AAATCACAAAAGGATGAAGT+AGG + chr1_2:8970990-8971009 None:intergenic 30.0%
CACTTAAAGAAAAGAAACCA+AGG + chr1_2:8971029-8971048 None:intergenic 30.0%
CCTGAAGATACAAAAAAGTA+AGG + chr1_2:8971059-8971078 None:intergenic 30.0%
!!! CCTTACTTTTTTGTATCTTC+AGG - chr1_2:8971056-8971075 Msa0054230:intron 30.0%
!!! TACTTTTTTGTATCTTCAGG+AGG - chr1_2:8971059-8971078 Msa0054230:intron 30.0%
TACAAACCATGTCTAATCTT+TGG + chr1_2:8971262-8971281 None:intergenic 30.0%
ACATACAAAGAGATACAAAG+AGG + chr1_2:8971321-8971340 None:intergenic 30.0%
CTTTGTATCTCTTTGTATGT+AGG - chr1_2:8971321-8971340 Msa0054230:intron 30.0%
TACTGCAAATCTGATGTAAA+AGG - chr1_2:8971383-8971402 Msa0054230:exon 30.0%
!!! AGGAGAATTTTTTTAGCAGT+GGG - chr1_2:8971432-8971451 Msa0054230:five_prime_UTR 30.0%
ACCTTACAAAGATGCTAAAG+TGG - chr1_2:8969879-8969898 Msa0054230:three_prime_UTR 35.0%
TCCACTTTAGCATCTTTGTA+AGG + chr1_2:8969883-8969902 None:intergenic 35.0%
GTATTCATCGCCCTTAATTT+GGG - chr1_2:8969922-8969941 Msa0054230:three_prime_UTR 35.0%
TAACATGCCTCCCAAATTAA+GGG + chr1_2:8969935-8969954 None:intergenic 35.0%
! GGTTCATTTTGTTGGATAGT+TGG - chr1_2:8970166-8970185 Msa0054230:three_prime_UTR 35.0%
AGGCAGTAACATGAATAACA+GGG + chr1_2:8970312-8970331 None:intergenic 35.0%
! CTTCAAGGTATTATGACAAG+AGG - chr1_2:8970517-8970536 Msa0054230:intron 35.0%
TAACATACAATGCTATGGGT+AGG + chr1_2:8970581-8970600 None:intergenic 35.0%
!!! TTTTTTGTATCTTCAGGAGG+TGG - chr1_2:8971062-8971081 Msa0054230:intron 35.0%
!!! AGTTTTTTGTCTTCTCTTGG+AGG - chr1_2:8971131-8971150 Msa0054230:intron 35.0%
TCTTCTCTTGGAGGAATTTA+AGG - chr1_2:8971140-8971159 Msa0054230:intron 35.0%
CTTGGAGGAATTTAAGGATT+TGG - chr1_2:8971146-8971165 Msa0054230:intron 35.0%
GTACTGTTAGCATGTTGTAA+TGG - chr1_2:8971168-8971187 Msa0054230:intron 35.0%
TGGTCACAAATGATCTCATT+AGG - chr1_2:8971188-8971207 Msa0054230:intron 35.0%
CATACTAAAAATGTTAGGGG+AGG - chr1_2:8971412-8971431 Msa0054230:five_prime_UTR 35.0%
!!! GAGGAGAATTTTTTTAGCAG+TGG - chr1_2:8971431-8971450 Msa0054230:five_prime_UTR 35.0%
!!! GGAGAATTTTTTTAGCAGTG+GGG - chr1_2:8971433-8971452 Msa0054230:five_prime_UTR 35.0%
ACCTTACAAAGATGCTAAAG+TGG - chr1_2:8969879-8969898 Msa0054230:three_prime_UTR 35.0%
TCCACTTTAGCATCTTTGTA+AGG + chr1_2:8969883-8969902 None:intergenic 35.0%
GTATTCATCGCCCTTAATTT+GGG - chr1_2:8969922-8969941 Msa0054230:three_prime_UTR 35.0%
TAACATGCCTCCCAAATTAA+GGG + chr1_2:8969935-8969954 None:intergenic 35.0%
! GGTTCATTTTGTTGGATAGT+TGG - chr1_2:8970166-8970185 Msa0054230:three_prime_UTR 35.0%
AGGCAGTAACATGAATAACA+GGG + chr1_2:8970312-8970331 None:intergenic 35.0%
! CTTCAAGGTATTATGACAAG+AGG - chr1_2:8970517-8970536 Msa0054230:intron 35.0%
TAACATACAATGCTATGGGT+AGG + chr1_2:8970581-8970600 None:intergenic 35.0%
!!! TTTTTTGTATCTTCAGGAGG+TGG - chr1_2:8971062-8971081 Msa0054230:intron 35.0%
!!! AGTTTTTTGTCTTCTCTTGG+AGG - chr1_2:8971131-8971150 Msa0054230:intron 35.0%
TCTTCTCTTGGAGGAATTTA+AGG - chr1_2:8971140-8971159 Msa0054230:intron 35.0%
CTTGGAGGAATTTAAGGATT+TGG - chr1_2:8971146-8971165 Msa0054230:intron 35.0%
GTACTGTTAGCATGTTGTAA+TGG - chr1_2:8971168-8971187 Msa0054230:intron 35.0%
TGGTCACAAATGATCTCATT+AGG - chr1_2:8971188-8971207 Msa0054230:intron 35.0%
CATACTAAAAATGTTAGGGG+AGG - chr1_2:8971412-8971431 Msa0054230:five_prime_UTR 35.0%
!!! GAGGAGAATTTTTTTAGCAG+TGG - chr1_2:8971431-8971450 Msa0054230:five_prime_UTR 35.0%
!!! GGAGAATTTTTTTAGCAGTG+GGG - chr1_2:8971433-8971452 Msa0054230:five_prime_UTR 35.0%
ATGCTAAAGTGGAGCTTCTT+GGG - chr1_2:8969890-8969909 Msa0054230:three_prime_UTR 40.0%
GGTATTCATCGCCCTTAATT+TGG - chr1_2:8969921-8969940 Msa0054230:three_prime_UTR 40.0%
GTAACATGCCTCCCAAATTA+AGG + chr1_2:8969936-8969955 None:intergenic 40.0%
! AATTTGGGAGGCATGTTACT+AGG - chr1_2:8969937-8969956 Msa0054230:three_prime_UTR 40.0%
CATGTTACTAGGTGTTTGGA+AGG - chr1_2:8969948-8969967 Msa0054230:three_prime_UTR 40.0%
AGGCTGATAAATTCACTGCT+AGG + chr1_2:8970105-8970124 None:intergenic 40.0%
GAGGCAGTAACATGAATAAC+AGG + chr1_2:8970313-8970332 None:intergenic 40.0%
AATGAATTGAGCTGCAGAAG+AGG + chr1_2:8970332-8970351 None:intergenic 40.0%
TCTTCTGCAGCTCAATTCAT+TGG - chr1_2:8970331-8970350 Msa0054230:intron 40.0%
CTTCTGCAGCTCAATTCATT+GGG - chr1_2:8970332-8970351 Msa0054230:intron 40.0%
AAAGAAAATCCAACCTGAGC+AGG + chr1_2:8970401-8970420 None:intergenic 40.0%
!!! TGCCTCTGGTTTTTTTTCCT+GGG + chr1_2:8970634-8970653 None:intergenic 40.0%
!!! TTGCCTCTGGTTTTTTTTCC+TGG + chr1_2:8970635-8970654 None:intergenic 40.0%
AAGCATGTAATCATTGCCTC+TGG + chr1_2:8970648-8970667 None:intergenic 40.0%
CACATGCCATAGTGAAGAAT+TGG - chr1_2:8970811-8970830 Msa0054230:intron 40.0%
CAGCTCATGGAAATCACAAA+AGG + chr1_2:8971000-8971019 None:intergenic 40.0%
! CTTAAGAACTGGAGCATCAA+CGG + chr1_2:8971113-8971132 None:intergenic 40.0%
! TCTTTGGTGACTGCAAGTAT+TGG + chr1_2:8971246-8971265 None:intergenic 40.0%
CAGTCACCAAAGATTAGACA+TGG - chr1_2:8971253-8971272 Msa0054230:intron 40.0%
ATGCTAAAGTGGAGCTTCTT+GGG - chr1_2:8969890-8969909 Msa0054230:three_prime_UTR 40.0%
GGTATTCATCGCCCTTAATT+TGG - chr1_2:8969921-8969940 Msa0054230:three_prime_UTR 40.0%
GTAACATGCCTCCCAAATTA+AGG + chr1_2:8969936-8969955 None:intergenic 40.0%
! AATTTGGGAGGCATGTTACT+AGG - chr1_2:8969937-8969956 Msa0054230:three_prime_UTR 40.0%
CATGTTACTAGGTGTTTGGA+AGG - chr1_2:8969948-8969967 Msa0054230:three_prime_UTR 40.0%
AGGCTGATAAATTCACTGCT+AGG + chr1_2:8970105-8970124 None:intergenic 40.0%
GAGGCAGTAACATGAATAAC+AGG + chr1_2:8970313-8970332 None:intergenic 40.0%
AATGAATTGAGCTGCAGAAG+AGG + chr1_2:8970332-8970351 None:intergenic 40.0%
TCTTCTGCAGCTCAATTCAT+TGG - chr1_2:8970331-8970350 Msa0054230:intron 40.0%
CTTCTGCAGCTCAATTCATT+GGG - chr1_2:8970332-8970351 Msa0054230:intron 40.0%
AAAGAAAATCCAACCTGAGC+AGG + chr1_2:8970401-8970420 None:intergenic 40.0%
!!! TGCCTCTGGTTTTTTTTCCT+GGG + chr1_2:8970634-8970653 None:intergenic 40.0%
!!! TTGCCTCTGGTTTTTTTTCC+TGG + chr1_2:8970635-8970654 None:intergenic 40.0%
AAGCATGTAATCATTGCCTC+TGG + chr1_2:8970648-8970667 None:intergenic 40.0%
CACATGCCATAGTGAAGAAT+TGG - chr1_2:8970811-8970830 Msa0054230:intron 40.0%
CAGCTCATGGAAATCACAAA+AGG + chr1_2:8971000-8971019 None:intergenic 40.0%
! CTTAAGAACTGGAGCATCAA+CGG + chr1_2:8971113-8971132 None:intergenic 40.0%
! TCTTTGGTGACTGCAAGTAT+TGG + chr1_2:8971246-8971265 None:intergenic 40.0%
CAGTCACCAAAGATTAGACA+TGG - chr1_2:8971253-8971272 Msa0054230:intron 40.0%
GATGCTAAAGTGGAGCTTCT+TGG - chr1_2:8969889-8969908 Msa0054230:three_prime_UTR 45.0%
AGGGCGATGAATACCAACTT+AGG + chr1_2:8969916-8969935 None:intergenic 45.0%
! TTCATCGCCCTTAATTTGGG+AGG - chr1_2:8969925-8969944 Msa0054230:three_prime_UTR 45.0%
GAGGCATGTTACTAGGTGTT+TGG - chr1_2:8969944-8969963 Msa0054230:three_prime_UTR 45.0%
GCAACAATATCTGTAGACCC+AGG - chr1_2:8970614-8970633 Msa0054230:intron 45.0%
GACCCAGGAAAAAAAACCAG+AGG - chr1_2:8970629-8970648 Msa0054230:intron 45.0%
GATGCTAAAGTGGAGCTTCT+TGG - chr1_2:8969889-8969908 Msa0054230:three_prime_UTR 45.0%
AGGGCGATGAATACCAACTT+AGG + chr1_2:8969916-8969935 None:intergenic 45.0%
! TTCATCGCCCTTAATTTGGG+AGG - chr1_2:8969925-8969944 Msa0054230:three_prime_UTR 45.0%
GAGGCATGTTACTAGGTGTT+TGG - chr1_2:8969944-8969963 Msa0054230:three_prime_UTR 45.0%
GCAACAATATCTGTAGACCC+AGG - chr1_2:8970614-8970633 Msa0054230:intron 45.0%
GACCCAGGAAAAAAAACCAG+AGG - chr1_2:8970629-8970648 Msa0054230:intron 45.0%
TTCCTACCCACACATCAGAC+TGG - chr1_2:8970360-8970379 Msa0054230:intron 50.0%
TCCTACCCACACATCAGACT+GGG - chr1_2:8970361-8970380 Msa0054230:intron 50.0%
TGACACCCAGTCTGATGTGT+GGG + chr1_2:8970369-8970388 None:intergenic 50.0%
ATGACACCCAGTCTGATGTG+TGG + chr1_2:8970370-8970389 None:intergenic 50.0%
TTCCTACCCACACATCAGAC+TGG - chr1_2:8970360-8970379 Msa0054230:intron 50.0%
TCCTACCCACACATCAGACT+GGG - chr1_2:8970361-8970380 Msa0054230:intron 50.0%
TGACACCCAGTCTGATGTGT+GGG + chr1_2:8970369-8970388 None:intergenic 50.0%
ATGACACCCAGTCTGATGTG+TGG + chr1_2:8970370-8970389 None:intergenic 50.0%
GGAGCTTCTTGGGCCTAAGT+TGG - chr1_2:8969900-8969919 Msa0054230:three_prime_UTR 55.0%
ACCCAGTCTGATGTGTGGGT+AGG + chr1_2:8970365-8970384 None:intergenic 55.0%
GTCATCCTTAGCACCTGCTC+AGG - chr1_2:8970385-8970404 Msa0054230:intron 55.0%
TCCTTAGCACCTGCTCAGGT+TGG - chr1_2:8970389-8970408 Msa0054230:intron 55.0%
TCCAACCTGAGCAGGTGCTA+AGG + chr1_2:8970393-8970412 None:intergenic 55.0%
TCCATGAGCTGCAACACCCT+TGG - chr1_2:8971009-8971028 Msa0054230:intron 55.0%
!! ACCAAGGGTGTTGCAGCTCA+TGG + chr1_2:8971013-8971032 None:intergenic 55.0%
! CTTCAGGAGGTGGAGTACTC+AGG - chr1_2:8971072-8971091 Msa0054230:intron 55.0%
! AGGAGGTGGAGTACTCAGGT+GGG - chr1_2:8971076-8971095 Msa0054230:intron 55.0%
GGAGCTTCTTGGGCCTAAGT+TGG - chr1_2:8969900-8969919 Msa0054230:three_prime_UTR 55.0%
ACCCAGTCTGATGTGTGGGT+AGG + chr1_2:8970365-8970384 None:intergenic 55.0%
GTCATCCTTAGCACCTGCTC+AGG - chr1_2:8970385-8970404 Msa0054230:intron 55.0%
TCCTTAGCACCTGCTCAGGT+TGG - chr1_2:8970389-8970408 Msa0054230:intron 55.0%
TCCAACCTGAGCAGGTGCTA+AGG + chr1_2:8970393-8970412 None:intergenic 55.0%
TCCATGAGCTGCAACACCCT+TGG - chr1_2:8971009-8971028 Msa0054230:intron 55.0%
!! ACCAAGGGTGTTGCAGCTCA+TGG + chr1_2:8971013-8971032 None:intergenic 55.0%
! CTTCAGGAGGTGGAGTACTC+AGG - chr1_2:8971072-8971091 Msa0054230:intron 55.0%
! AGGAGGTGGAGTACTCAGGT+GGG - chr1_2:8971076-8971095 Msa0054230:intron 55.0%
! CAGGAGGTGGAGTACTCAGG+TGG - chr1_2:8971075-8971094 Msa0054230:intron 60.0%
! GGAGGTGGAGTACTCAGGTG+GGG - chr1_2:8971077-8971096 Msa0054230:intron 60.0%
! GAGGTGGAGTACTCAGGTGG+GGG - chr1_2:8971078-8971097 Msa0054230:intron 60.0%
! CAGGAGGTGGAGTACTCAGG+TGG - chr1_2:8971075-8971094 Msa0054230:intron 60.0%
! GGAGGTGGAGTACTCAGGTG+GGG - chr1_2:8971077-8971096 Msa0054230:intron 60.0%
! GAGGTGGAGTACTCAGGTGG+GGG - chr1_2:8971078-8971097 Msa0054230:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 8969872 8971465 8969872 ID=Msa0054230;Name=Msa0054230
chr1_2 mRNA 8969872 8971465 8969872 ID=Msa0054230-mRNA-1;Parent=Msa0054230;Name=Msa0054230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=77|1|0.66|1|1|1|3|349|41
chr1_2 exon 8969872 8970259 8969872 ID=Msa0054230-mRNA-1:exon:3210;Parent=Msa0054230-mRNA-1
chr1_2 exon 8970931 8970996 8970931 ID=Msa0054230-mRNA-1:exon:3209;Parent=Msa0054230-mRNA-1
chr1_2 exon 8971368 8971465 8971368 ID=Msa0054230-mRNA-1:exon:3208;Parent=Msa0054230-mRNA-1
chr1_2 five_prime_UTR 8971389 8971465 8971389 ID=Msa0054230-mRNA-1:five_prime_utr;Parent=Msa0054230-mRNA-1
chr1_2 CDS 8971368 8971388 8971368 ID=Msa0054230-mRNA-1:cds;Parent=Msa0054230-mRNA-1
chr1_2 CDS 8970931 8970996 8970931 ID=Msa0054230-mRNA-1:cds;Parent=Msa0054230-mRNA-1
chr1_2 CDS 8970221 8970259 8970221 ID=Msa0054230-mRNA-1:cds;Parent=Msa0054230-mRNA-1
chr1_2 three_prime_UTR 8969872 8970220 8969872 ID=Msa0054230-mRNA-1:three_prime_utr;Parent=Msa0054230-mRNA-1
Gene Sequence

>Msa0054230

ATGTTACTAGGTGTTTGGAAGCTCAATTCATTGGGACTGCTTCCTACCCACACATCAGACTGGGTGTCATCCTTAGCACCTGCTCAGGAGGTGGAGTACTCAGGTGGGGGTCTAAATTTCCGTTGA
Protein sequence

>Msa0054230

MLLGVWKLNSLGLLPTHTSDWVSSLAPAQEVEYSGGGLNFR*