Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0040450 | Msa1291840 | 0.801692 | 7.988199e-49 | -8.615850e-47 |
| Msa0682900 | Msa1291840 | 0.811864 | 5.656737e-51 | -8.615850e-47 |
| Msa0693380 | Msa1291840 | 0.801503 | 8.733030e-49 | -8.615850e-47 |
| Msa0744040 | Msa1291840 | 0.801120 | 1.046171e-48 | -8.615850e-47 |
| Msa0309770 | Msa1291840 | 0.800950 | 1.133190e-48 | -8.615850e-47 |
| Msa1187840 | Msa1291840 | 0.807641 | 4.576428e-50 | -8.615850e-47 |
| Msa0547360 | Msa1291840 | 0.810820 | 9.529911e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGTTATGTTCTAATTTA+AGG | 0.098914 | 8_3:-47401083 | Msa1291840:three_prime_UTR |
| TCTCTCTCTTTCTCTGCAAT+TGG | 0.294169 | 8_3:+47401221 | None:intergenic |
| GATTTAAATTGAAAATTCAT+AGG | 0.360114 | 8_3:+47401017 | None:intergenic |
| CAATTGGAAACACATTTCCT+TGG | 0.401795 | 8_3:+47401237 | None:intergenic |
| ACTAGTTCATAGAGCTGAAC+TGG | 0.426499 | 8_3:-47401304 | Msa1291840:intron |
| ATAATTAGAAGTAGTGTGAT+TGG | 0.435113 | 8_3:-47401261 | Msa1291840:CDS |
| AATTGGAAACACATTTCCTT+GGG | 0.470075 | 8_3:+47401238 | None:intergenic |
| GCTGAACTGGTAGAGAATCA+AGG | 0.538682 | 8_3:-47401291 | Msa1291840:CDS |
| GAAGTAGTGTGATTGGCCCA+AGG | 0.638653 | 8_3:-47401254 | Msa1291840:CDS |
| AAGCAACTAAAGAAATGCAG+AGG | 0.682925 | 8_3:-47401188 | Msa1291840:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTATAGAAAATTCACTAT+TGG | - | chr8_3:47401146-47401165 | Msa1291840:CDS | 15.0% |
| !! | TTTATAGAAAATTCACTATT+GGG | - | chr8_3:47401147-47401166 | Msa1291840:CDS | 15.0% |
| !! | TATTCATATAAATGTAGAAA+AGG | + | chr8_3:47401241-47401260 | None:intergenic | 15.0% |
| !! | AACACATTAAAAAGAAAAAA+AGG | + | chr8_3:47401349-47401368 | None:intergenic | 15.0% |
| !!! | TATTTTTATTGCAATTTTCT+TGG | - | chr8_3:47401607-47401626 | Msa1291840:intron | 15.0% |
| !!! | ATTTTTATTGCAATTTTCTT+GGG | - | chr8_3:47401608-47401627 | Msa1291840:intron | 15.0% |
| !! | GATTTAAATTGAAAATTCAT+AGG | + | chr8_3:47401695-47401714 | None:intergenic | 15.0% |
| !!! | AAATTATTATTTTGCTCCTA+TGG | - | chr8_3:47401175-47401194 | Msa1291840:CDS | 20.0% |
| !!! | TTCTTTTTAATGTGTTATTC+TGG | - | chr8_3:47401353-47401372 | Msa1291840:intron | 20.0% |
| ! | TCTATAAATGATTAGTACAC+TGG | + | chr8_3:47401135-47401154 | None:intergenic | 25.0% |
| ! | AAAATGAGTAAAACATGGTA+CGG | + | chr8_3:47401204-47401223 | None:intergenic | 25.0% |
| ! | ATAATTAGAAGTAGTGTGAT+TGG | - | chr8_3:47401448-47401467 | Msa1291840:intron | 25.0% |
| ! | TTGGGTTATGTTCTAATTTA+AGG | - | chr8_3:47401626-47401645 | Msa1291840:intron | 25.0% |
| TACTCAATTTGATGTACTGT+TGG | - | chr8_3:47401025-47401044 | Msa1291840:three_prime_UTR | 30.0% | |
| ACTGTTGGATCATAATATGT+TGG | - | chr8_3:47401040-47401059 | Msa1291840:three_prime_UTR | 30.0% | |
| GATTAGTACACTGGTAATAT+AGG | + | chr8_3:47401126-47401145 | None:intergenic | 30.0% | |
| ! | GTACCATGTTTTACTCATTT+TGG | - | chr8_3:47401203-47401222 | Msa1291840:CDS | 30.0% |
| TGACCAAAATGAGTAAAACA+TGG | + | chr8_3:47401209-47401228 | None:intergenic | 30.0% | |
| AATTGGAAACACATTTCCTT+GGG | + | chr8_3:47401474-47401493 | None:intergenic | 30.0% | |
| GAATATGATTGTCTCAGTCT+CGG | - | chr8_3:47401256-47401275 | Msa1291840:CDS | 35.0% | |
| CAATTGGAAACACATTTCCT+TGG | + | chr8_3:47401475-47401494 | None:intergenic | 35.0% | |
| AAGCAACTAAAGAAATGCAG+AGG | - | chr8_3:47401521-47401540 | Msa1291840:intron | 35.0% | |
| ! | GTCTCGGTTTTTATTGCTGT+TGG | - | chr8_3:47401272-47401291 | Msa1291840:CDS | 40.0% |
| ACTAGTTCATAGAGCTGAAC+TGG | - | chr8_3:47401405-47401424 | Msa1291840:intron | 40.0% | |
| TCTCTCTCTTTCTCTGCAAT+TGG | + | chr8_3:47401491-47401510 | None:intergenic | 40.0% | |
| AAACATGGTACGGAAGCCAT+AGG | + | chr8_3:47401194-47401213 | None:intergenic | 45.0% | |
| GCTGAACTGGTAGAGAATCA+AGG | - | chr8_3:47401418-47401437 | Msa1291840:intron | 45.0% | |
| ! | GAAGTAGTGTGATTGGCCCA+AGG | - | chr8_3:47401455-47401474 | Msa1291840:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 47401006 | 47401725 | 47401006 | ID=Msa1291840;Name=Msa1291840 |
| chr8_3 | mRNA | 47401006 | 47401725 | 47401006 | ID=Msa1291840-mRNA-1;Parent=Msa1291840;Name=Msa1291840-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0|0.5|0.5|1|1|2|137|61 |
| chr8_3 | exon | 47401006 | 47401321 | 47401006 | ID=Msa1291840-mRNA-1:exon:13231;Parent=Msa1291840-mRNA-1 |
| chr8_3 | exon | 47401719 | 47401725 | 47401719 | ID=Msa1291840-mRNA-1:exon:13230;Parent=Msa1291840-mRNA-1 |
| chr8_3 | CDS | 47401719 | 47401725 | 47401719 | ID=Msa1291840-mRNA-1:cds;Parent=Msa1291840-mRNA-1 |
| chr8_3 | CDS | 47401143 | 47401321 | 47401143 | ID=Msa1291840-mRNA-1:cds;Parent=Msa1291840-mRNA-1 |
| chr8_3 | three_prime_UTR | 47401006 | 47401142 | 47401006 | ID=Msa1291840-mRNA-1:three_prime_utr;Parent=Msa1291840-mRNA-1 |
| Gene Sequence |
| Protein sequence |
