AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1297000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297000 MtrunA17_Chr4g0053911 98.283 233 4 0 1 233 1 233 1.22e-170 469
Msa1297000 MtrunA17_Chr5g0401401 71.008 238 57 5 1 233 1 231 9.16e-112 320
Msa1297000 MtrunA17_Chr7g0215281 61.417 127 46 1 1 124 3 129 1.79e-54 176
Msa1297000 MtrunA17_Chr1g0207471 61.029 136 50 2 1 133 1 136 2.27e-53 170
Msa1297000 MtrunA17_Chr6g0457431 55.319 141 51 1 1 141 3 131 1.44e-52 167
Msa1297000 MtrunA17_Chr6g0469271 60.550 109 41 1 1 107 3 111 1.77e-46 154
Msa1297000 MtrunA17_Chr6g0470651 51.667 120 54 3 1 118 3 120 1.00e-41 142
Msa1297000 MtrunA17_Chr6g0470631 49.194 124 59 3 1 122 3 124 1.18e-40 139
Msa1297000 MtrunA17_Chr1g0209841 48.387 124 50 3 1 122 3 114 2.34e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297000 AT3G49940.1 57.937 252 80 7 1 232 1 246 2.62e-83 248
Msa1297000 AT4G37540.1 57.831 249 80 8 1 233 1 240 6.85e-83 247
Msa1297000 AT5G67420.2 54.331 254 91 6 1 233 1 250 1.26e-76 232
Msa1297000 AT5G67420.1 54.331 254 91 6 1 233 1 250 1.26e-76 232
Msa1297000 AT1G67100.1 67.273 110 36 0 1 110 3 112 1.14e-53 172
Msa1297000 AT3G02550.1 47.727 176 74 2 1 166 3 170 3.84e-52 170
Msa1297000 AT1G68510.1 61.947 113 41 1 1 111 3 115 1.17e-48 160

Find 98 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACTCATGTTTCTATTT+TGG 0.155235 8_3:-56886246 None:intergenic
AAGATTCGCGATCCGAATTT+CGG 0.249558 8_3:+56886793 Msa1297000:CDS
TTACCTGGTCTCTGTGGTTC+GGG 0.275915 8_3:-56886424 None:intergenic
GTTGGGGTATAAATAAATAA+AGG 0.296968 8_3:-56886106 None:intergenic
GAATAGAAAGAAAGTGGTTT+AGG 0.300622 8_3:-56886162 None:intergenic
GACTTCTTTGGACTGGAAAC+TGG 0.306937 8_3:+56886629 Msa1297000:CDS
GTAGCGTGGCCTTGAGCTTC+TGG 0.312181 8_3:-56886343 None:intergenic
ATTTGATATTCCACGGTTAC+AGG 0.313336 8_3:-56887306 None:intergenic
ACGGTGCTGTTGGACTTCTT+TGG 0.315165 8_3:+56886617 Msa1297000:CDS
AGAAAGTGGTTTAGGAATAA+TGG 0.315956 8_3:-56886154 None:intergenic
TTTACCGGAGCTGACACTTC+TGG 0.333923 8_3:+56886705 Msa1297000:CDS
AGATTCGCGATCCGAATTTC+GGG 0.342540 8_3:+56886794 Msa1297000:CDS
ACGGTGGCGCTGATGCTATT+TGG 0.350213 8_3:+56886770 Msa1297000:CDS
TCTAGTAATAAGGTGTGTTC+TGG 0.372758 8_3:+56886832 Msa1297000:CDS
ATTACCTGGTCTCTGTGGTT+CGG 0.410585 8_3:-56886425 None:intergenic
TTGGGGTATAAATAAATAAA+GGG 0.424281 8_3:-56886105 None:intergenic
GAGCTCACGATCTAGTAATA+AGG 0.427686 8_3:+56886822 Msa1297000:CDS
AAGAGAGAATAGAAAGAAAG+TGG 0.431602 8_3:-56886168 None:intergenic
AGTAAACTATGGTTGAGTTA+AGG 0.435665 8_3:-56886050 None:intergenic
GTCGATGACGTCGGAGGAAT+CGG 0.439790 8_3:+56886972 Msa1297000:CDS
TGGCACGCTTAGGCCTTTAC+CGG 0.448239 8_3:+56886690 Msa1297000:CDS
CAGAAAATCCTCATATGAAA+AGG 0.454062 8_3:-56887068 None:intergenic
AGAAGAAGAGGAGGTGTGGT+TGG 0.462946 8_3:-56886074 None:intergenic
TTCTTCGTGGTGGCACGCTT+AGG 0.479220 8_3:+56886680 Msa1297000:CDS
GCTGTTGGACTTCTTTGGAC+TGG 0.482667 8_3:+56886622 Msa1297000:CDS
GACGTCATCGACGGTGAACC+AGG 0.485410 8_3:-56886961 None:intergenic
GCAAAATTCTTTGGTCGAGC+TGG 0.485866 8_3:+56886376 Msa1297000:CDS
ATGCGAATTGAAGTGATGTT+GGG 0.486771 8_3:-56886123 None:intergenic
GTGTGGTTGGTAGTAAACTA+TGG 0.487162 8_3:-56886061 None:intergenic
AGGGGAGGAAGGAGAAGAAG+AGG 0.493231 8_3:-56886086 None:intergenic
AATAGAAACATGAGTTGCAA+TGG 0.497518 8_3:+56886250 Msa1297000:exon
TTGTGGTGAAAAGAGTAAAA+CGG 0.498145 8_3:+56887153 Msa1297000:three_prime_UTR
AATGCGAATTGAAGTGATGT+TGG 0.500278 8_3:-56886124 None:intergenic
GTTTCGATCCATTGCAAACA+AGG 0.507273 8_3:-56886319 None:intergenic
TCTCTAGAGAAATCCTGAAC+CGG 0.508750 8_3:-56887206 None:intergenic
TTCGGGGACGTTGGAAATGA+AGG 0.510532 8_3:-56886407 None:intergenic
AGTCCAACAGCACCGTTCAC+AGG 0.514245 8_3:-56886610 None:intergenic
GGGTGCCGAGTTCTTCGAAA+AGG 0.515559 8_3:+56886271 Msa1297000:CDS
GCAGTGGAGGAACGACGGCC+TGG 0.518874 8_3:+56886943 Msa1297000:CDS
AGAAGTGTCAGCTCCGGTAA+AGG 0.524390 8_3:-56886703 None:intergenic
TAAATAAATAAAGGGGAGGA+AGG 0.524788 8_3:-56886097 None:intergenic
ATTTAATATTTGATATTCCA+CGG 0.529132 8_3:-56887313 None:intergenic
ATAGAAACATGAGTTGCAAT+GGG 0.529336 8_3:+56886251 Msa1297000:exon
AAGGAGAAGAAGAGGAGGTG+TGG 0.529924 8_3:-56886078 None:intergenic
GGTGGAGTCACGGTGGTGAT+CGG 0.543236 8_3:+56887034 Msa1297000:CDS
GGAGGAAGGAGAAGAAGAGG+AGG 0.543266 8_3:-56886083 None:intergenic
TAAGGTTAGTTGCAAGCAAG+TGG 0.549359 8_3:-56886032 None:intergenic
ATTGGAGATCGGTGGAGTCA+CGG 0.549508 8_3:+56887024 Msa1297000:CDS
AGAGAGTCAGAGAAAGATGA+TGG 0.551093 8_3:+56887224 Msa1297000:three_prime_UTR
AACCCTGTGAACGGTGCTGT+TGG 0.557626 8_3:+56886607 Msa1297000:CDS
AGCAAAACAGAGAAACGTTA+CGG 0.559764 8_3:-56887119 None:intergenic
GCGGTTGAGACGGTTCTTCG+TGG 0.561357 8_3:+56886667 Msa1297000:CDS
AAGGTTAGTTGCAAGCAAGT+GGG 0.561944 8_3:-56886031 None:intergenic
GTTTGAAGCGTGTGGAAGAA+CGG 0.563405 8_3:+56886582 Msa1297000:CDS
TCTCTGTGGTTCGGGGACGT+TGG 0.563762 8_3:-56886416 None:intergenic
TTGAAACTGGGATTGGAGAT+CGG 0.567262 8_3:+56887013 Msa1297000:CDS
TGGAAGCAAACGGAGGAGAT+CGG 0.569877 8_3:+56886852 Msa1297000:CDS
TGCCACTGATGAAGCTTCTG+AGG 0.578795 8_3:+56886741 Msa1297000:CDS
GTGGTTTAGGAATAATGGAA+TGG 0.579027 8_3:-56886149 None:intergenic
AAATCAAGATTAATCGCCGC+GGG 0.581245 8_3:-56886892 None:intergenic
AGGTGTGTTCTGGAAGCAAA+CGG 0.583068 8_3:+56886842 Msa1297000:CDS
GAAGCTTCTGAGGCGGAAGA+CGG 0.587046 8_3:+56886751 Msa1297000:CDS
TCGCGATCCGAATTTCGGGT+TGG 0.591044 8_3:+56886798 Msa1297000:CDS
TTGTCAAGCAGCGGTTGAGA+CGG 0.594446 8_3:+56886657 Msa1297000:CDS
CACTGATGAAGCTTCTGAGG+CGG 0.596477 8_3:+56886744 Msa1297000:CDS
AAGTGGGGAGAGAGACGTGA+AGG 0.599979 8_3:-56886015 None:intergenic
TCTCTACTGTTTGAAGCGTG+TGG 0.600037 8_3:+56886574 Msa1297000:CDS
GTCCAACAGCACCGTTCACA+GGG 0.600320 8_3:-56886609 None:intergenic
GCTTCATCAGTGGCAGTGAG+AGG 0.602040 8_3:-56886733 None:intergenic
TTTGCTTACAATACTCATTG+TGG 0.609288 8_3:+56887136 Msa1297000:three_prime_UTR
AGGTTAGTTGCAAGCAAGTG+GGG 0.609783 8_3:-56886030 None:intergenic
CGCCTCAGAAGCTTCATCAG+TGG 0.613409 8_3:-56886743 None:intergenic
AGAGAAAGATGATGGTTCGT+CGG 0.615967 8_3:+56887232 Msa1297000:three_prime_UTR
GTCCCCGAACCACAGAGACC+AGG 0.617602 8_3:+56886421 Msa1297000:CDS
AAATAATTACCTGGTCTCTG+TGG 0.626571 8_3:-56886430 None:intergenic
CTGGCACGTTTGTCAAGCAG+CGG 0.631827 8_3:+56886648 Msa1297000:CDS
GAGATCGGAGGAGTTTGTCA+AGG 0.633312 8_3:+56886867 Msa1297000:CDS
TACCTGGTCTCTGTGGTTCG+GGG 0.637914 8_3:-56886423 None:intergenic
GCTTCTGAGGCGGAAGACGG+TGG 0.641101 8_3:+56886754 Msa1297000:CDS
TGCGAATTGAAGTGATGTTG+GGG 0.644823 8_3:-56886122 None:intergenic
GTTGAGACGGTTCTTCGTGG+TGG 0.647601 8_3:+56886670 Msa1297000:CDS
GATTCCTCCGACGTCATCGA+CGG 0.648497 8_3:-56886970 None:intergenic
GGAGTTTGTCAAGGTTCCCG+CGG 0.651001 8_3:+56886876 Msa1297000:CDS
TGGGGTATAAATAAATAAAG+GGG 0.654291 8_3:-56886104 None:intergenic
TGGTTCACCGTCGATGACGT+CGG 0.655686 8_3:+56886963 Msa1297000:CDS
AGAAGGCAGTGGAGGAACGA+CGG 0.656112 8_3:+56886938 Msa1297000:CDS
ATCGAAACACCAGAAGCTCA+AGG 0.656960 8_3:+56886334 Msa1297000:CDS
AAGCAAACGGAGGAGATCGG+AGG 0.657755 8_3:+56886855 Msa1297000:CDS
GATGTGTAAACCTGTAACCG+TGG 0.659840 8_3:+56887296 Msa1297000:three_prime_UTR
GGAGGAATCGGTGACGACGA+CGG 0.660492 8_3:+56886984 Msa1297000:CDS
AAACTGGGATTGGAGATCGG+TGG 0.660979 8_3:+56887016 Msa1297000:CDS
AGAACGGTTAACCCTGTGAA+CGG 0.663812 8_3:+56886598 Msa1297000:CDS
TTGCTACGAAAACAGTAGCG+TGG 0.670232 8_3:-56886357 None:intergenic
GGTATAAATAAATAAAGGGG+AGG 0.672012 8_3:-56886101 None:intergenic
TAAATCAAGATTAATCGCCG+CGG 0.697247 8_3:-56886893 None:intergenic
TTCACCGTCGATGACGTCGG+AGG 0.699966 8_3:+56886966 Msa1297000:CDS
TGTGTTCTGGAAGCAAACGG+AGG 0.716901 8_3:+56886845 Msa1297000:CDS
GGAGATCGGTGGAGTCACGG+TGG 0.745894 8_3:+56887027 Msa1297000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATATTTGATATTCCA+CGG - chr8_3:56887316-56887335 None:intergenic 15.0%
!! ATTTAATATTTGATATTCCA+CGG - chr8_3:56887316-56887335 None:intergenic 15.0%
!! TTGGGGTATAAATAAATAAA+GGG - chr8_3:56886108-56886127 None:intergenic 20.0%
!! CAATCTTTCTCTTATATTTA+CGG - chr8_3:56886471-56886490 None:intergenic 20.0%
!! TTGGGGTATAAATAAATAAA+GGG - chr8_3:56886108-56886127 None:intergenic 20.0%
!! CAATCTTTCTCTTATATTTA+CGG - chr8_3:56886471-56886490 None:intergenic 20.0%
! TGGGGTATAAATAAATAAAG+GGG - chr8_3:56886107-56886126 None:intergenic 25.0%
! GTTGGGGTATAAATAAATAA+AGG - chr8_3:56886109-56886128 None:intergenic 25.0%
! CGTAAATATAAGAGAAAGAT+TGG + chr8_3:56886469-56886488 Msa1297000:intron 25.0%
! TGGGGTATAAATAAATAAAG+GGG - chr8_3:56886107-56886126 None:intergenic 25.0%
! GTTGGGGTATAAATAAATAA+AGG - chr8_3:56886109-56886128 None:intergenic 25.0%
! CGTAAATATAAGAGAAAGAT+TGG + chr8_3:56886469-56886488 Msa1297000:intron 25.0%
AGTAAACTATGGTTGAGTTA+AGG - chr8_3:56886053-56886072 None:intergenic 30.0%
TAAATAAATAAAGGGGAGGA+AGG - chr8_3:56886100-56886119 None:intergenic 30.0%
GGTATAAATAAATAAAGGGG+AGG - chr8_3:56886104-56886123 None:intergenic 30.0%
AGAAAGTGGTTTAGGAATAA+TGG - chr8_3:56886157-56886176 None:intergenic 30.0%
GAATAGAAAGAAAGTGGTTT+AGG - chr8_3:56886165-56886184 None:intergenic 30.0%
AAGAGAGAATAGAAAGAAAG+TGG - chr8_3:56886171-56886190 None:intergenic 30.0%
!! TGCAACTCATGTTTCTATTT+TGG - chr8_3:56886249-56886268 None:intergenic 30.0%
AATAGAAACATGAGTTGCAA+TGG + chr8_3:56886250-56886269 Msa1297000:exon 30.0%
ATAGAAACATGAGTTGCAAT+GGG + chr8_3:56886251-56886270 Msa1297000:exon 30.0%
! GTTTTCGTAGCAAAATTCTT+TGG + chr8_3:56886367-56886386 Msa1297000:CDS 30.0%
AGACCAGGTAATTATTTAAC+CGG + chr8_3:56886436-56886455 Msa1297000:intron 30.0%
CAGAAAATCCTCATATGAAA+AGG - chr8_3:56887071-56887090 None:intergenic 30.0%
TTTGCTTACAATACTCATTG+TGG + chr8_3:56887136-56887155 Msa1297000:three_prime_UTR 30.0%
TTGTGGTGAAAAGAGTAAAA+CGG + chr8_3:56887153-56887172 Msa1297000:three_prime_UTR 30.0%
!! AGTAAAACGGTTTTGTAGTT+CGG + chr8_3:56887166-56887185 Msa1297000:three_prime_UTR 30.0%
AGTAAACTATGGTTGAGTTA+AGG - chr8_3:56886053-56886072 None:intergenic 30.0%
TAAATAAATAAAGGGGAGGA+AGG - chr8_3:56886100-56886119 None:intergenic 30.0%
GGTATAAATAAATAAAGGGG+AGG - chr8_3:56886104-56886123 None:intergenic 30.0%
AGAAAGTGGTTTAGGAATAA+TGG - chr8_3:56886157-56886176 None:intergenic 30.0%
GAATAGAAAGAAAGTGGTTT+AGG - chr8_3:56886165-56886184 None:intergenic 30.0%
AAGAGAGAATAGAAAGAAAG+TGG - chr8_3:56886171-56886190 None:intergenic 30.0%
!! TGCAACTCATGTTTCTATTT+TGG - chr8_3:56886249-56886268 None:intergenic 30.0%
AATAGAAACATGAGTTGCAA+TGG + chr8_3:56886250-56886269 Msa1297000:exon 30.0%
ATAGAAACATGAGTTGCAAT+GGG + chr8_3:56886251-56886270 Msa1297000:exon 30.0%
! GTTTTCGTAGCAAAATTCTT+TGG + chr8_3:56886367-56886386 Msa1297000:CDS 30.0%
AGACCAGGTAATTATTTAAC+CGG + chr8_3:56886436-56886455 Msa1297000:intron 30.0%
CAGAAAATCCTCATATGAAA+AGG - chr8_3:56887071-56887090 None:intergenic 30.0%
TTTGCTTACAATACTCATTG+TGG + chr8_3:56887136-56887155 Msa1297000:three_prime_UTR 30.0%
TTGTGGTGAAAAGAGTAAAA+CGG + chr8_3:56887153-56887172 Msa1297000:three_prime_UTR 30.0%
!! AGTAAAACGGTTTTGTAGTT+CGG + chr8_3:56887166-56887185 Msa1297000:three_prime_UTR 30.0%
ATGCGAATTGAAGTGATGTT+GGG - chr8_3:56886126-56886145 None:intergenic 35.0%
AATGCGAATTGAAGTGATGT+TGG - chr8_3:56886127-56886146 None:intergenic 35.0%
! GTGGTTTAGGAATAATGGAA+TGG - chr8_3:56886152-56886171 None:intergenic 35.0%
!! TTTTACGACCTTGTTTGCAA+TGG + chr8_3:56886311-56886330 Msa1297000:CDS 35.0%
AAATAATTACCTGGTCTCTG+TGG - chr8_3:56886433-56886452 None:intergenic 35.0%
AGACCGGTTAAATAATTACC+TGG - chr8_3:56886442-56886461 None:intergenic 35.0%
TCTAGTAATAAGGTGTGTTC+TGG + chr8_3:56886832-56886851 Msa1297000:CDS 35.0%
TAAATCAAGATTAATCGCCG+CGG - chr8_3:56886896-56886915 None:intergenic 35.0%
ACTGCCTTCTGTTGAAAAAT+CGG - chr8_3:56886928-56886947 None:intergenic 35.0%
! GTTCTCAACCTTTTCATATG+AGG + chr8_3:56887060-56887079 Msa1297000:CDS 35.0%
AGCAAAACAGAGAAACGTTA+CGG - chr8_3:56887122-56887141 None:intergenic 35.0%
ATTTGATATTCCACGGTTAC+AGG - chr8_3:56887309-56887328 None:intergenic 35.0%
ATGCGAATTGAAGTGATGTT+GGG - chr8_3:56886126-56886145 None:intergenic 35.0%
AATGCGAATTGAAGTGATGT+TGG - chr8_3:56886127-56886146 None:intergenic 35.0%
! GTGGTTTAGGAATAATGGAA+TGG - chr8_3:56886152-56886171 None:intergenic 35.0%
!! TTTTACGACCTTGTTTGCAA+TGG + chr8_3:56886311-56886330 Msa1297000:CDS 35.0%
AAATAATTACCTGGTCTCTG+TGG - chr8_3:56886433-56886452 None:intergenic 35.0%
AGACCGGTTAAATAATTACC+TGG - chr8_3:56886442-56886461 None:intergenic 35.0%
TCTAGTAATAAGGTGTGTTC+TGG + chr8_3:56886832-56886851 Msa1297000:CDS 35.0%
TAAATCAAGATTAATCGCCG+CGG - chr8_3:56886896-56886915 None:intergenic 35.0%
ACTGCCTTCTGTTGAAAAAT+CGG - chr8_3:56886928-56886947 None:intergenic 35.0%
! GTTCTCAACCTTTTCATATG+AGG + chr8_3:56887060-56887079 Msa1297000:CDS 35.0%
AGCAAAACAGAGAAACGTTA+CGG - chr8_3:56887122-56887141 None:intergenic 35.0%
ATTTGATATTCCACGGTTAC+AGG - chr8_3:56887309-56887328 None:intergenic 35.0%
! AAGGTTAGTTGCAAGCAAGT+GGG - chr8_3:56886034-56886053 None:intergenic 40.0%
! TAAGGTTAGTTGCAAGCAAG+TGG - chr8_3:56886035-56886054 None:intergenic 40.0%
!! GTGTGGTTGGTAGTAAACTA+TGG - chr8_3:56886064-56886083 None:intergenic 40.0%
TGCGAATTGAAGTGATGTTG+GGG - chr8_3:56886125-56886144 None:intergenic 40.0%
! TGCAACCTTTTCGAAGAACT+CGG - chr8_3:56886279-56886298 None:intergenic 40.0%
GTTTCGATCCATTGCAAACA+AGG - chr8_3:56886322-56886341 None:intergenic 40.0%
!! ATATTTACGGACTCTGAGAC+CGG - chr8_3:56886458-56886477 None:intergenic 40.0%
AAGATTCGCGATCCGAATTT+CGG + chr8_3:56886793-56886812 Msa1297000:CDS 40.0%
GAGCTCACGATCTAGTAATA+AGG + chr8_3:56886822-56886841 Msa1297000:CDS 40.0%
AAATCAAGATTAATCGCCGC+GGG - chr8_3:56886895-56886914 None:intergenic 40.0%
!! GATTTTTCAACAGAAGGCAG+TGG + chr8_3:56886927-56886946 Msa1297000:CDS 40.0%
TTGAAACTGGGATTGGAGAT+CGG + chr8_3:56887013-56887032 Msa1297000:CDS 40.0%
! GGTGAATTTTCGTGAGTTAC+CGG + chr8_3:56887187-56887206 Msa1297000:three_prime_UTR 40.0%
! TCTCTAGAGAAATCCTGAAC+CGG - chr8_3:56887209-56887228 None:intergenic 40.0%
AGAGAGTCAGAGAAAGATGA+TGG + chr8_3:56887224-56887243 Msa1297000:three_prime_UTR 40.0%
! AGAGAAAGATGATGGTTCGT+CGG + chr8_3:56887232-56887251 Msa1297000:three_prime_UTR 40.0%
! AAGGTTAGTTGCAAGCAAGT+GGG - chr8_3:56886034-56886053 None:intergenic 40.0%
! TAAGGTTAGTTGCAAGCAAG+TGG - chr8_3:56886035-56886054 None:intergenic 40.0%
!! GTGTGGTTGGTAGTAAACTA+TGG - chr8_3:56886064-56886083 None:intergenic 40.0%
TGCGAATTGAAGTGATGTTG+GGG - chr8_3:56886125-56886144 None:intergenic 40.0%
! TGCAACCTTTTCGAAGAACT+CGG - chr8_3:56886279-56886298 None:intergenic 40.0%
GTTTCGATCCATTGCAAACA+AGG - chr8_3:56886322-56886341 None:intergenic 40.0%
!! ATATTTACGGACTCTGAGAC+CGG - chr8_3:56886458-56886477 None:intergenic 40.0%
AAGATTCGCGATCCGAATTT+CGG + chr8_3:56886793-56886812 Msa1297000:CDS 40.0%
GAGCTCACGATCTAGTAATA+AGG + chr8_3:56886822-56886841 Msa1297000:CDS 40.0%
AAATCAAGATTAATCGCCGC+GGG - chr8_3:56886895-56886914 None:intergenic 40.0%
!! GATTTTTCAACAGAAGGCAG+TGG + chr8_3:56886927-56886946 Msa1297000:CDS 40.0%
TTGAAACTGGGATTGGAGAT+CGG + chr8_3:56887013-56887032 Msa1297000:CDS 40.0%
! GGTGAATTTTCGTGAGTTAC+CGG + chr8_3:56887187-56887206 Msa1297000:three_prime_UTR 40.0%
! TCTCTAGAGAAATCCTGAAC+CGG - chr8_3:56887209-56887228 None:intergenic 40.0%
AGAGAGTCAGAGAAAGATGA+TGG + chr8_3:56887224-56887243 Msa1297000:three_prime_UTR 40.0%
! AGAGAAAGATGATGGTTCGT+CGG + chr8_3:56887232-56887251 Msa1297000:three_prime_UTR 40.0%
! AGGTTAGTTGCAAGCAAGTG+GGG - chr8_3:56886033-56886052 None:intergenic 45.0%
ATCGAAACACCAGAAGCTCA+AGG + chr8_3:56886334-56886353 Msa1297000:CDS 45.0%
TTGCTACGAAAACAGTAGCG+TGG - chr8_3:56886360-56886379 None:intergenic 45.0%
GCAAAATTCTTTGGTCGAGC+TGG + chr8_3:56886376-56886395 Msa1297000:CDS 45.0%
ATTACCTGGTCTCTGTGGTT+CGG - chr8_3:56886428-56886447 None:intergenic 45.0%
! TCTCTACTGTTTGAAGCGTG+TGG + chr8_3:56886574-56886593 Msa1297000:CDS 45.0%
! GTTTGAAGCGTGTGGAAGAA+CGG + chr8_3:56886582-56886601 Msa1297000:CDS 45.0%
AGAACGGTTAACCCTGTGAA+CGG + chr8_3:56886598-56886617 Msa1297000:CDS 45.0%
! GACTTCTTTGGACTGGAAAC+TGG + chr8_3:56886629-56886648 Msa1297000:CDS 45.0%
AGATTCGCGATCCGAATTTC+GGG + chr8_3:56886794-56886813 Msa1297000:CDS 45.0%
!! AGGTGTGTTCTGGAAGCAAA+CGG + chr8_3:56886842-56886861 Msa1297000:CDS 45.0%
!!! GACGCCGATTTTTCAACAGA+AGG + chr8_3:56886921-56886940 Msa1297000:CDS 45.0%
! TTTTCAACAGAAGGCAGTGG+AGG + chr8_3:56886930-56886949 Msa1297000:CDS 45.0%
!!! GCGTGTTTTGAAACTGGGAT+TGG + chr8_3:56887006-56887025 Msa1297000:CDS 45.0%
!!! TTTTCGTGAGTTACCGGTTC+AGG + chr8_3:56887193-56887212 Msa1297000:three_prime_UTR 45.0%
GATGTGTAAACCTGTAACCG+TGG + chr8_3:56887296-56887315 Msa1297000:three_prime_UTR 45.0%
! AGGTTAGTTGCAAGCAAGTG+GGG - chr8_3:56886033-56886052 None:intergenic 45.0%
ATCGAAACACCAGAAGCTCA+AGG + chr8_3:56886334-56886353 Msa1297000:CDS 45.0%
TTGCTACGAAAACAGTAGCG+TGG - chr8_3:56886360-56886379 None:intergenic 45.0%
GCAAAATTCTTTGGTCGAGC+TGG + chr8_3:56886376-56886395 Msa1297000:CDS 45.0%
ATTACCTGGTCTCTGTGGTT+CGG - chr8_3:56886428-56886447 None:intergenic 45.0%
! TCTCTACTGTTTGAAGCGTG+TGG + chr8_3:56886574-56886593 Msa1297000:CDS 45.0%
! GTTTGAAGCGTGTGGAAGAA+CGG + chr8_3:56886582-56886601 Msa1297000:CDS 45.0%
AGAACGGTTAACCCTGTGAA+CGG + chr8_3:56886598-56886617 Msa1297000:CDS 45.0%
! GACTTCTTTGGACTGGAAAC+TGG + chr8_3:56886629-56886648 Msa1297000:CDS 45.0%
AGATTCGCGATCCGAATTTC+GGG + chr8_3:56886794-56886813 Msa1297000:CDS 45.0%
!! AGGTGTGTTCTGGAAGCAAA+CGG + chr8_3:56886842-56886861 Msa1297000:CDS 45.0%
!!! GACGCCGATTTTTCAACAGA+AGG + chr8_3:56886921-56886940 Msa1297000:CDS 45.0%
! TTTTCAACAGAAGGCAGTGG+AGG + chr8_3:56886930-56886949 Msa1297000:CDS 45.0%
!!! GCGTGTTTTGAAACTGGGAT+TGG + chr8_3:56887006-56887025 Msa1297000:CDS 45.0%
!!! TTTTCGTGAGTTACCGGTTC+AGG + chr8_3:56887193-56887212 Msa1297000:three_prime_UTR 45.0%
GATGTGTAAACCTGTAACCG+TGG + chr8_3:56887296-56887315 Msa1297000:three_prime_UTR 45.0%
! AGAAGAAGAGGAGGTGTGGT+TGG - chr8_3:56886077-56886096 None:intergenic 50.0%
AAGGAGAAGAAGAGGAGGTG+TGG - chr8_3:56886081-56886100 None:intergenic 50.0%
TTCGGGGACGTTGGAAATGA+AGG - chr8_3:56886410-56886429 None:intergenic 50.0%
TTACCTGGTCTCTGTGGTTC+GGG - chr8_3:56886427-56886446 None:intergenic 50.0%
!! ACGGTGCTGTTGGACTTCTT+TGG + chr8_3:56886617-56886636 Msa1297000:CDS 50.0%
GCTGTTGGACTTCTTTGGAC+TGG + chr8_3:56886622-56886641 Msa1297000:CDS 50.0%
TTGTCAAGCAGCGGTTGAGA+CGG + chr8_3:56886657-56886676 Msa1297000:CDS 50.0%
!! AGAAGTGTCAGCTCCGGTAA+AGG - chr8_3:56886706-56886725 None:intergenic 50.0%
TTTACCGGAGCTGACACTTC+TGG + chr8_3:56886705-56886724 Msa1297000:CDS 50.0%
TGCCACTGATGAAGCTTCTG+AGG + chr8_3:56886741-56886760 Msa1297000:CDS 50.0%
! CACTGATGAAGCTTCTGAGG+CGG + chr8_3:56886744-56886763 Msa1297000:CDS 50.0%
!! TGTGTTCTGGAAGCAAACGG+AGG + chr8_3:56886845-56886864 Msa1297000:CDS 50.0%
TGGAAGCAAACGGAGGAGAT+CGG + chr8_3:56886852-56886871 Msa1297000:CDS 50.0%
GAGATCGGAGGAGTTTGTCA+AGG + chr8_3:56886867-56886886 Msa1297000:CDS 50.0%
!!! ACGACGGCGTGTTTTGAAAC+TGG + chr8_3:56887000-56887019 Msa1297000:CDS 50.0%
!!! CGACGGCGTGTTTTGAAACT+GGG + chr8_3:56887001-56887020 Msa1297000:CDS 50.0%
AAACTGGGATTGGAGATCGG+TGG + chr8_3:56887016-56887035 Msa1297000:CDS 50.0%
ATTGGAGATCGGTGGAGTCA+CGG + chr8_3:56887024-56887043 Msa1297000:CDS 50.0%
! AGAAGAAGAGGAGGTGTGGT+TGG - chr8_3:56886077-56886096 None:intergenic 50.0%
AAGGAGAAGAAGAGGAGGTG+TGG - chr8_3:56886081-56886100 None:intergenic 50.0%
TTCGGGGACGTTGGAAATGA+AGG - chr8_3:56886410-56886429 None:intergenic 50.0%
TTACCTGGTCTCTGTGGTTC+GGG - chr8_3:56886427-56886446 None:intergenic 50.0%
!! ACGGTGCTGTTGGACTTCTT+TGG + chr8_3:56886617-56886636 Msa1297000:CDS 50.0%
GCTGTTGGACTTCTTTGGAC+TGG + chr8_3:56886622-56886641 Msa1297000:CDS 50.0%
TTGTCAAGCAGCGGTTGAGA+CGG + chr8_3:56886657-56886676 Msa1297000:CDS 50.0%
!! AGAAGTGTCAGCTCCGGTAA+AGG - chr8_3:56886706-56886725 None:intergenic 50.0%
TTTACCGGAGCTGACACTTC+TGG + chr8_3:56886705-56886724 Msa1297000:CDS 50.0%
TGCCACTGATGAAGCTTCTG+AGG + chr8_3:56886741-56886760 Msa1297000:CDS 50.0%
! CACTGATGAAGCTTCTGAGG+CGG + chr8_3:56886744-56886763 Msa1297000:CDS 50.0%
!! TGTGTTCTGGAAGCAAACGG+AGG + chr8_3:56886845-56886864 Msa1297000:CDS 50.0%
TGGAAGCAAACGGAGGAGAT+CGG + chr8_3:56886852-56886871 Msa1297000:CDS 50.0%
GAGATCGGAGGAGTTTGTCA+AGG + chr8_3:56886867-56886886 Msa1297000:CDS 50.0%
!!! ACGACGGCGTGTTTTGAAAC+TGG + chr8_3:56887000-56887019 Msa1297000:CDS 50.0%
!!! CGACGGCGTGTTTTGAAACT+GGG + chr8_3:56887001-56887020 Msa1297000:CDS 50.0%
AAACTGGGATTGGAGATCGG+TGG + chr8_3:56887016-56887035 Msa1297000:CDS 50.0%
ATTGGAGATCGGTGGAGTCA+CGG + chr8_3:56887024-56887043 Msa1297000:CDS 50.0%
AAGTGGGGAGAGAGACGTGA+AGG - chr8_3:56886018-56886037 None:intergenic 55.0%
GGAGGAAGGAGAAGAAGAGG+AGG - chr8_3:56886086-56886105 None:intergenic 55.0%
AGGGGAGGAAGGAGAAGAAG+AGG - chr8_3:56886089-56886108 None:intergenic 55.0%
!! GGGTGCCGAGTTCTTCGAAA+AGG + chr8_3:56886271-56886290 Msa1297000:CDS 55.0%
TACCTGGTCTCTGTGGTTCG+GGG - chr8_3:56886426-56886445 None:intergenic 55.0%
!! AACCCTGTGAACGGTGCTGT+TGG + chr8_3:56886607-56886626 Msa1297000:CDS 55.0%
GTCCAACAGCACCGTTCACA+GGG - chr8_3:56886612-56886631 None:intergenic 55.0%
AGTCCAACAGCACCGTTCAC+AGG - chr8_3:56886613-56886632 None:intergenic 55.0%
CTGGCACGTTTGTCAAGCAG+CGG + chr8_3:56886648-56886667 Msa1297000:CDS 55.0%
GTTGAGACGGTTCTTCGTGG+TGG + chr8_3:56886670-56886689 Msa1297000:CDS 55.0%
TTCTTCGTGGTGGCACGCTT+AGG + chr8_3:56886680-56886699 Msa1297000:CDS 55.0%
TGGCACGCTTAGGCCTTTAC+CGG + chr8_3:56886690-56886709 Msa1297000:CDS 55.0%
GCTTCATCAGTGGCAGTGAG+AGG - chr8_3:56886736-56886755 None:intergenic 55.0%
CGCCTCAGAAGCTTCATCAG+TGG - chr8_3:56886746-56886765 None:intergenic 55.0%
!! GAAGCTTCTGAGGCGGAAGA+CGG + chr8_3:56886751-56886770 Msa1297000:CDS 55.0%
!! ACGGTGGCGCTGATGCTATT+TGG + chr8_3:56886770-56886789 Msa1297000:CDS 55.0%
! TCGCGATCCGAATTTCGGGT+TGG + chr8_3:56886798-56886817 Msa1297000:CDS 55.0%
GAGCTCGCCAACCCGAAATT+CGG - chr8_3:56886808-56886827 None:intergenic 55.0%
AAGCAAACGGAGGAGATCGG+AGG + chr8_3:56886855-56886874 Msa1297000:CDS 55.0%
GGAGTTTGTCAAGGTTCCCG+CGG + chr8_3:56886876-56886895 Msa1297000:CDS 55.0%
AGAAGGCAGTGGAGGAACGA+CGG + chr8_3:56886938-56886957 Msa1297000:CDS 55.0%
TGGTTCACCGTCGATGACGT+CGG + chr8_3:56886963-56886982 Msa1297000:CDS 55.0%
GATTCCTCCGACGTCATCGA+CGG - chr8_3:56886973-56886992 None:intergenic 55.0%
GTCGATGACGTCGGAGGAAT+CGG + chr8_3:56886972-56886991 Msa1297000:CDS 55.0%
AAGTGGGGAGAGAGACGTGA+AGG - chr8_3:56886018-56886037 None:intergenic 55.0%
GGAGGAAGGAGAAGAAGAGG+AGG - chr8_3:56886086-56886105 None:intergenic 55.0%
AGGGGAGGAAGGAGAAGAAG+AGG - chr8_3:56886089-56886108 None:intergenic 55.0%
!! GGGTGCCGAGTTCTTCGAAA+AGG + chr8_3:56886271-56886290 Msa1297000:CDS 55.0%
TACCTGGTCTCTGTGGTTCG+GGG - chr8_3:56886426-56886445 None:intergenic 55.0%
!! AACCCTGTGAACGGTGCTGT+TGG + chr8_3:56886607-56886626 Msa1297000:CDS 55.0%
GTCCAACAGCACCGTTCACA+GGG - chr8_3:56886612-56886631 None:intergenic 55.0%
AGTCCAACAGCACCGTTCAC+AGG - chr8_3:56886613-56886632 None:intergenic 55.0%
CTGGCACGTTTGTCAAGCAG+CGG + chr8_3:56886648-56886667 Msa1297000:CDS 55.0%
GTTGAGACGGTTCTTCGTGG+TGG + chr8_3:56886670-56886689 Msa1297000:CDS 55.0%
TTCTTCGTGGTGGCACGCTT+AGG + chr8_3:56886680-56886699 Msa1297000:CDS 55.0%
TGGCACGCTTAGGCCTTTAC+CGG + chr8_3:56886690-56886709 Msa1297000:CDS 55.0%
GCTTCATCAGTGGCAGTGAG+AGG - chr8_3:56886736-56886755 None:intergenic 55.0%
CGCCTCAGAAGCTTCATCAG+TGG - chr8_3:56886746-56886765 None:intergenic 55.0%
!! GAAGCTTCTGAGGCGGAAGA+CGG + chr8_3:56886751-56886770 Msa1297000:CDS 55.0%
!! ACGGTGGCGCTGATGCTATT+TGG + chr8_3:56886770-56886789 Msa1297000:CDS 55.0%
! TCGCGATCCGAATTTCGGGT+TGG + chr8_3:56886798-56886817 Msa1297000:CDS 55.0%
GAGCTCGCCAACCCGAAATT+CGG - chr8_3:56886808-56886827 None:intergenic 55.0%
AAGCAAACGGAGGAGATCGG+AGG + chr8_3:56886855-56886874 Msa1297000:CDS 55.0%
GGAGTTTGTCAAGGTTCCCG+CGG + chr8_3:56886876-56886895 Msa1297000:CDS 55.0%
AGAAGGCAGTGGAGGAACGA+CGG + chr8_3:56886938-56886957 Msa1297000:CDS 55.0%
TGGTTCACCGTCGATGACGT+CGG + chr8_3:56886963-56886982 Msa1297000:CDS 55.0%
GATTCCTCCGACGTCATCGA+CGG - chr8_3:56886973-56886992 None:intergenic 55.0%
GTCGATGACGTCGGAGGAAT+CGG + chr8_3:56886972-56886991 Msa1297000:CDS 55.0%
! GTAGCGTGGCCTTGAGCTTC+TGG - chr8_3:56886346-56886365 None:intergenic 60.0%
! TCTCTGTGGTTCGGGGACGT+TGG - chr8_3:56886419-56886438 None:intergenic 60.0%
GCGGTTGAGACGGTTCTTCG+TGG + chr8_3:56886667-56886686 Msa1297000:CDS 60.0%
!! GCGTCCAGAAGTGTCAGCTC+CGG - chr8_3:56886712-56886731 None:intergenic 60.0%
GACGTCATCGACGGTGAACC+AGG - chr8_3:56886964-56886983 None:intergenic 60.0%
TTCACCGTCGATGACGTCGG+AGG + chr8_3:56886966-56886985 Msa1297000:CDS 60.0%
GGAGGAATCGGTGACGACGA+CGG + chr8_3:56886984-56887003 Msa1297000:CDS 60.0%
GGTGGAGTCACGGTGGTGAT+CGG + chr8_3:56887034-56887053 Msa1297000:CDS 60.0%
! GTAGCGTGGCCTTGAGCTTC+TGG - chr8_3:56886346-56886365 None:intergenic 60.0%
! TCTCTGTGGTTCGGGGACGT+TGG - chr8_3:56886419-56886438 None:intergenic 60.0%
GCGGTTGAGACGGTTCTTCG+TGG + chr8_3:56886667-56886686 Msa1297000:CDS 60.0%
!! GCGTCCAGAAGTGTCAGCTC+CGG - chr8_3:56886712-56886731 None:intergenic 60.0%
GACGTCATCGACGGTGAACC+AGG - chr8_3:56886964-56886983 None:intergenic 60.0%
TTCACCGTCGATGACGTCGG+AGG + chr8_3:56886966-56886985 Msa1297000:CDS 60.0%
GGAGGAATCGGTGACGACGA+CGG + chr8_3:56886984-56887003 Msa1297000:CDS 60.0%
GGTGGAGTCACGGTGGTGAT+CGG + chr8_3:56887034-56887053 Msa1297000:CDS 60.0%
GTCCCCGAACCACAGAGACC+AGG + chr8_3:56886421-56886440 Msa1297000:CDS 65.0%
!! GCTTCTGAGGCGGAAGACGG+TGG + chr8_3:56886754-56886773 Msa1297000:CDS 65.0%
GGAGATCGGTGGAGTCACGG+TGG + chr8_3:56887027-56887046 Msa1297000:CDS 65.0%
GTCCCCGAACCACAGAGACC+AGG + chr8_3:56886421-56886440 Msa1297000:CDS 65.0%
!! GCTTCTGAGGCGGAAGACGG+TGG + chr8_3:56886754-56886773 Msa1297000:CDS 65.0%
GGAGATCGGTGGAGTCACGG+TGG + chr8_3:56887027-56887046 Msa1297000:CDS 65.0%
GCAGTGGAGGAACGACGGCC+TGG + chr8_3:56886943-56886962 Msa1297000:CDS 70.0%
GCAGTGGAGGAACGACGGCC+TGG + chr8_3:56886943-56886962 Msa1297000:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 56886008 56887365 56886008 ID=Msa1297000;Name=Msa1297000
chr8_3 mRNA 56886008 56887365 56886008 ID=Msa1297000-mRNA-1;Parent=Msa1297000;Name=Msa1297000-mRNA-1;_AED=0.04;_eAED=0.04;_QI=251|1|1|1|1|1|2|285|233
chr8_3 exon 56886008 56886442 56886008 ID=Msa1297000-mRNA-1:exon:16313;Parent=Msa1297000-mRNA-1
chr8_3 exon 56886563 56887365 56886563 ID=Msa1297000-mRNA-1:exon:16314;Parent=Msa1297000-mRNA-1
chr8_3 five_prime_UTR 56886008 56886258 56886008 ID=Msa1297000-mRNA-1:five_prime_utr;Parent=Msa1297000-mRNA-1
chr8_3 CDS 56886259 56886442 56886259 ID=Msa1297000-mRNA-1:cds;Parent=Msa1297000-mRNA-1
chr8_3 CDS 56886563 56887080 56886563 ID=Msa1297000-mRNA-1:cds;Parent=Msa1297000-mRNA-1
chr8_3 three_prime_UTR 56887081 56887365 56887081 ID=Msa1297000-mRNA-1:three_prime_utr;Parent=Msa1297000-mRNA-1
Gene Sequence

>Msa1297000

ATGAGTTGCAATGGGTGCCGAGTTCTTCGAAAAGGTTGCAGTGAGTCATGTATTTTACGACCTTGTTTGCAATGGATCGAAACACCAGAAGCTCAAGGCCACGCTACTGTTTTCGTAGCAAAATTCTTTGGTCGAGCTGGTCTCATGTCCTTCATTTCCAACGTCCCCGAACCACAGAGACCAGCTCTGTTTCAGTCTCTACTGTTTGAAGCGTGTGGAAGAACGGTTAACCCTGTGAACGGTGCTGTTGGACTTCTTTGGACTGGAAACTGGCACGTTTGTCAAGCAGCGGTTGAGACGGTTCTTCGTGGTGGCACGCTTAGGCCTTTACCGGAGCTGACACTTCTGGACGCGCCTCTCACTGCCACTGATGAAGCTTCTGAGGCGGAAGACGGTGGCGCTGATGCTATTTGGAAGATTCGCGATCCGAATTTCGGGTTGGCGAGCTCACGATCTAGTAATAAGGTGTGTTCTGGAAGCAAACGGAGGAGATCGGAGGAGTTTGTCAAGGTTCCCGCGGCGATTAATCTTGATTTACGATTGACGCCGATTTTTCAACAGAAGGCAGTGGAGGAACGACGGCCTGGTTCACCGTCGATGACGTCGGAGGAATCGGTGACGACGACGGCGTGTTTTGAAACTGGGATTGGAGATCGGTGGAGTCACGGTGGTGATCGGAAAGTTCTCAACCTTTTCATATGA
Protein sequence

>Msa1297000

MSCNGCRVLRKGCSESCILRPCLQWIETPEAQGHATVFVAKFFGRAGLMSFISNVPEPQRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLRGGTLRPLPELTLLDAPLTATDEASEAEDGGADAIWKIRDPNFGLASSRSSNKVCSGSKRRRSEEFVKVPAAINLDLRLTPIFQQKAVEERRPGSPSMTSEESVTTTACFETGIGDRWSHGGDRKVLNLFI*