Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1297060 | AES90722.1 | 94.958 | 119 | 6 | 0 | 3 | 121 | 4 | 122 | 3.18e-77 | 238 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1297060 | G7JE21 | 94.958 | 119 | 6 | 0 | 3 | 121 | 4 | 122 | 1.52e-77 | 238 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1297060 | Msa1407220 | 0.832952 | 7.045645e-56 | -8.615850e-47 |
| Msa1206050 | Msa1297060 | 0.834565 | 2.784881e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1297060 | MtrunA17_Chr4g0054061 | 94.958 | 119 | 6 | 0 | 3 | 121 | 4 | 122 | 2.92e-81 | 238 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1297060 | AT3G49890.1 | 39.167 | 120 | 64 | 3 | 1 | 120 | 1 | 111 | 2.13e-18 | 77.8 |
| Msa1297060 | AT3G49890.2 | 39.167 | 120 | 64 | 3 | 1 | 120 | 1 | 111 | 2.22e-18 | 78.2 |
Find 34 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTAATCTTTGGGGATTT+AGG | 0.177270 | 8_3:+56984060 | None:intergenic |
| ATTATCATTATGCTTCTTCT+TGG | 0.221055 | 8_3:+56984089 | None:intergenic |
| GCTTAATCTTTGGGGATTTA+GGG | 0.228273 | 8_3:+56984061 | None:intergenic |
| AGTTGGTAGTGCTTAATCTT+TGG | 0.259629 | 8_3:+56984051 | None:intergenic |
| GTTGGTAGTGCTTAATCTTT+GGG | 0.273130 | 8_3:+56984052 | None:intergenic |
| CTTCTTGGTGTCATTGTTGT+TGG | 0.276148 | 8_3:+56984104 | None:intergenic |
| TTGGCAGGCACAAAAGCTTT+TGG | 0.334252 | 8_3:-56983709 | Msa1297060:intron |
| TATGTCACTTCCTTCTTGAA+TGG | 0.336482 | 8_3:-56984138 | Msa1297060:CDS |
| GCAGCCATTGATTCCGTTGC+CGG | 0.343503 | 8_3:-56984171 | Msa1297060:CDS |
| CTGCTACAGTAGCCATTGTT+TGG | 0.353921 | 8_3:+56984228 | None:intergenic |
| CTGGTTTAGAATGTCTAAAC+AGG | 0.391452 | 8_3:+56983598 | None:intergenic |
| ATGCCGCAATCATCGACATT+AGG | 0.437333 | 8_3:+56983624 | None:intergenic |
| AGTGACATAGGAGGAGGTTC+CGG | 0.442671 | 8_3:+56984152 | None:intergenic |
| CGACATTAGGATCCTCATCT+AGG | 0.450341 | 8_3:+56983637 | None:intergenic |
| GATTAAGCACTACCAACTTA+AGG | 0.464419 | 8_3:-56984046 | Msa1297060:intron |
| CTTGGTGTCATTGTTGTTGG+TGG | 0.472021 | 8_3:+56984107 | None:intergenic |
| TTTAGAATTGGACCTTAAGT+TGG | 0.504850 | 8_3:+56984034 | None:intergenic |
| GGTTCCGGCAACGGAATCAA+TGG | 0.515860 | 8_3:+56984167 | None:intergenic |
| CTGTTTAGACATTCTAAACC+AGG | 0.547528 | 8_3:-56983597 | Msa1297060:CDS |
| GGTGTCATTGTTGTTGGTGG+CGG | 0.554093 | 8_3:+56984110 | None:intergenic |
| ATGAAGACGGTGACGCAGCA+TGG | 0.564455 | 8_3:-56984197 | Msa1297060:CDS |
| ATAGGAGGAGGTTCCGGCAA+CGG | 0.566478 | 8_3:+56984158 | None:intergenic |
| TTAGGATCCTCATCTAGGAC+AGG | 0.568212 | 8_3:+56983642 | None:intergenic |
| ATTCAAGAAGGAAGTGACAT+AGG | 0.569799 | 8_3:+56984140 | None:intergenic |
| GAAGGAAGTGACATAGGAGG+AGG | 0.580901 | 8_3:+56984146 | None:intergenic |
| ATCTATTCCTGTCCTAGATG+AGG | 0.593312 | 8_3:-56983649 | Msa1297060:CDS |
| GATCCTAATGTCGATGATTG+CGG | 0.595195 | 8_3:-56983627 | Msa1297060:CDS |
| AATGGCTACTGTAGCAGCAG+TGG | 0.604176 | 8_3:-56984222 | Msa1297060:CDS |
| AGCAGCAGTGGCGATGAAGA+CGG | 0.605287 | 8_3:-56984210 | Msa1297060:CDS |
| GGCGGAAAATCCATTCAAGA+AGG | 0.609902 | 8_3:+56984128 | None:intergenic |
| TTGGTAGTGCTTAATCTTTG+GGG | 0.616398 | 8_3:+56984053 | None:intergenic |
| CAAGAAGGAAGTGACATAGG+AGG | 0.628507 | 8_3:+56984143 | None:intergenic |
| ATGGCAGAAGAACCAAACAA+TGG | 0.702147 | 8_3:-56984240 | Msa1297060:CDS |
| AAACCTGAAAGAGTGAACAA+TGG | 0.716102 | 8_3:-56984259 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGCAAAAATAAAATAAAATT+AGG | - | chr8_3:56983860-56983879 | Msa1297060:intron | 10.0% |
| !!! | ATAAAATAAAATTAGGATTT+TGG | - | chr8_3:56983867-56983886 | Msa1297060:intron | 10.0% |
| !!! | AAAAATGAGAAATTTAGAAT+TGG | + | chr8_3:56983788-56983807 | None:intergenic | 15.0% |
| !!! | AAAACTGAATTTTGTTTTGT+GGG | + | chr8_3:56983914-56983933 | None:intergenic | 20.0% |
| !!! | AAAAACTGAATTTTGTTTTG+TGG | + | chr8_3:56983915-56983934 | None:intergenic | 20.0% |
| !! | TTTATGTTGTGTATGTTTAT+TGG | - | chr8_3:56984079-56984098 | Msa1297060:CDS | 20.0% |
| ! | ATTATCATTATGCTTCTTCT+TGG | + | chr8_3:56983721-56983740 | None:intergenic | 25.0% |
| ! | GTAACAAAAAAACATGAACT+TGG | + | chr8_3:56983942-56983961 | None:intergenic | 25.0% |
| !!! | AGTTCATGTTTTTTTGTTAC+TGG | - | chr8_3:56983942-56983961 | Msa1297060:intron | 25.0% |
| !! | ATTTGCAATTTTGCAAAAGA+AGG | + | chr8_3:56983995-56984014 | None:intergenic | 25.0% |
| !! | ATTTTTCTTCTCATGGTTTT+TGG | - | chr8_3:56984047-56984066 | Msa1297060:CDS | 25.0% |
| ! | TTTAGAATTGGACCTTAAGT+TGG | + | chr8_3:56983776-56983795 | None:intergenic | 30.0% |
| TGGAATTTGAATTACGTTTG+TGG | - | chr8_3:56983962-56983981 | Msa1297060:intron | 30.0% | |
| GGAATTTGAATTACGTTTGT+GGG | - | chr8_3:56983963-56983982 | Msa1297060:intron | 30.0% | |
| ! | ACTGTACATTTTTCTTCTCA+TGG | - | chr8_3:56984040-56984059 | Msa1297060:intron | 30.0% |
| ! | TTTTGATCATGCAGGTAAAT+TGG | - | chr8_3:56984239-56984258 | Msa1297060:CDS | 30.0% |
| ATTCAAGAAGGAAGTGACAT+AGG | + | chr8_3:56983670-56983689 | None:intergenic | 35.0% | |
| ! | TATGTCACTTCCTTCTTGAA+TGG | - | chr8_3:56983669-56983688 | Msa1297060:CDS | 35.0% |
| ! | GCTTAATCTTTGGGGATTTA+GGG | + | chr8_3:56983749-56983768 | None:intergenic | 35.0% |
| ! | TGCTTAATCTTTGGGGATTT+AGG | + | chr8_3:56983750-56983769 | None:intergenic | 35.0% |
| !! | TTGGTAGTGCTTAATCTTTG+GGG | + | chr8_3:56983757-56983776 | None:intergenic | 35.0% |
| !! | GTTGGTAGTGCTTAATCTTT+GGG | + | chr8_3:56983758-56983777 | None:intergenic | 35.0% |
| !! | AGTTGGTAGTGCTTAATCTT+TGG | + | chr8_3:56983759-56983778 | None:intergenic | 35.0% |
| GATTAAGCACTACCAACTTA+AGG | - | chr8_3:56983761-56983780 | Msa1297060:intron | 35.0% | |
| !!! | GAATTTTGTTTTGTGGGCAA+TGG | + | chr8_3:56983908-56983927 | None:intergenic | 35.0% |
| TGTTGTGTATGTTTATTGGC+AGG | - | chr8_3:56984083-56984102 | Msa1297060:CDS | 35.0% | |
| ! | AAAGCTTTTGGATGACATAG+TGG | - | chr8_3:56984110-56984129 | Msa1297060:CDS | 35.0% |
| ! | CTGGTTTAGAATGTCTAAAC+AGG | + | chr8_3:56984212-56984231 | None:intergenic | 35.0% |
| CTGTTTAGACATTCTAAACC+AGG | - | chr8_3:56984210-56984229 | Msa1297060:CDS | 35.0% | |
| GCATGATCAAAAACTATTCC+TGG | + | chr8_3:56984231-56984250 | None:intergenic | 35.0% | |
| ! | GGAATAGTTTTTGATCATGC+AGG | - | chr8_3:56984231-56984250 | Msa1297060:CDS | 35.0% |
| ATGGCAGAAGAACCAAACAA+TGG | - | chr8_3:56983567-56983586 | Msa1297060:CDS | 40.0% | |
| !! | CTTCTTGGTGTCATTGTTGT+TGG | + | chr8_3:56983706-56983725 | None:intergenic | 40.0% |
| ATCTATTCCTGTCCTAGATG+AGG | - | chr8_3:56984158-56984177 | Msa1297060:CDS | 40.0% | |
| GATCCTAATGTCGATGATTG+CGG | - | chr8_3:56984180-56984199 | Msa1297060:CDS | 40.0% | |
| CTGCTACAGTAGCCATTGTT+TGG | + | chr8_3:56983582-56983601 | None:intergenic | 45.0% | |
| CAAGAAGGAAGTGACATAGG+AGG | + | chr8_3:56983667-56983686 | None:intergenic | 45.0% | |
| GGCGGAAAATCCATTCAAGA+AGG | + | chr8_3:56983682-56983701 | None:intergenic | 45.0% | |
| !! | CTTGGTGTCATTGTTGTTGG+TGG | + | chr8_3:56983703-56983722 | None:intergenic | 45.0% |
| ! | TTGGCAGGCACAAAAGCTTT+TGG | - | chr8_3:56984098-56984117 | Msa1297060:CDS | 45.0% |
| ! | TTAGGATCCTCATCTAGGAC+AGG | + | chr8_3:56984168-56984187 | None:intergenic | 45.0% |
| CGACATTAGGATCCTCATCT+AGG | + | chr8_3:56984173-56984192 | None:intergenic | 45.0% | |
| ATGCCGCAATCATCGACATT+AGG | + | chr8_3:56984186-56984205 | None:intergenic | 45.0% | |
| AATGGCTACTGTAGCAGCAG+TGG | - | chr8_3:56983585-56983604 | Msa1297060:CDS | 50.0% | |
| AGTGACATAGGAGGAGGTTC+CGG | + | chr8_3:56983658-56983677 | None:intergenic | 50.0% | |
| GAAGGAAGTGACATAGGAGG+AGG | + | chr8_3:56983664-56983683 | None:intergenic | 50.0% | |
| !! | GGTGTCATTGTTGTTGGTGG+CGG | + | chr8_3:56983700-56983719 | None:intergenic | 50.0% |
| AGCAGCAGTGGCGATGAAGA+CGG | - | chr8_3:56983597-56983616 | Msa1297060:CDS | 55.0% | |
| ATGAAGACGGTGACGCAGCA+TGG | - | chr8_3:56983610-56983629 | Msa1297060:CDS | 55.0% | |
| !! | GCAGCCATTGATTCCGTTGC+CGG | - | chr8_3:56983636-56983655 | Msa1297060:CDS | 55.0% |
| GGTTCCGGCAACGGAATCAA+TGG | + | chr8_3:56983643-56983662 | None:intergenic | 55.0% | |
| ATAGGAGGAGGTTCCGGCAA+CGG | + | chr8_3:56983652-56983671 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 56983560 | 56984269 | 56983560 | ID=Msa1297060;Name=Msa1297060 |
| chr8_3 | mRNA | 56983560 | 56984269 | 56983560 | ID=Msa1297060-mRNA-1;Parent=Msa1297060;Name=Msa1297060-mRNA-1;_AED=0.07;_eAED=0.07;_QI=7|1|0.5|1|1|1|2|0|126 |
| chr8_3 | exon | 56983560 | 56983724 | 56983560 | ID=Msa1297060-mRNA-1:exon:16318;Parent=Msa1297060-mRNA-1 |
| chr8_3 | exon | 56984047 | 56984269 | 56984047 | ID=Msa1297060-mRNA-1:exon:16317;Parent=Msa1297060-mRNA-1 |
| chr8_3 | five_prime_UTR | 56984263 | 56984269 | 56984263 | ID=Msa1297060-mRNA-1:five_prime_utr;Parent=Msa1297060-mRNA-1 |
| chr8_3 | CDS | 56984047 | 56984262 | 56984047 | ID=Msa1297060-mRNA-1:cds;Parent=Msa1297060-mRNA-1 |
| chr8_3 | CDS | 56983560 | 56983724 | 56983560 | ID=Msa1297060-mRNA-1:cds;Parent=Msa1297060-mRNA-1 |
| Gene Sequence |
| Protein sequence |
