AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1297130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297130 MtrunA17_Chr4g0054151 100.000 136 0 0 1 136 4 139 9.03e-97 274
Msa1297130 MtrunA17_Chr4g0054161 100.000 136 0 0 1 136 1 136 1.05e-96 274
Msa1297130 MtrunA17_Chr8g0363741 100.000 136 0 0 1 136 1 136 1.05e-96 274
Msa1297130 MtrunA17_Chr1g0156021 100.000 136 0 0 1 136 1 136 1.05e-96 274
Msa1297130 MtrunA17_Chr8g0388121 99.265 136 1 0 1 136 1 136 3.83e-96 272
Msa1297130 MtrunA17_Chr5g0410931 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr5g0410951 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr8g0383851 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr8g0383781 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr8g0390361 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr2g0295191 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr7g0237661 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr4g0033041 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr7g0218971 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr4g0046301 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr8g0364801 97.059 136 4 0 1 136 1 136 2.60e-93 265
Msa1297130 MtrunA17_Chr2g0319141 92.647 136 10 0 1 136 1 136 3.29e-90 257
Msa1297130 MtrunA17_Chr2g0319151 92.647 136 10 0 1 136 1 136 3.29e-90 257
Msa1297130 MtrunA17_Chr2g0319131 86.301 146 10 1 1 136 1 146 3.00e-84 243
Msa1297130 MtrunA17_Chr2g0319121 85.294 136 18 2 1 135 1 135 1.40e-76 223
Msa1297130 MtrunA17_Chr7g0218961 83.036 112 19 0 25 136 2 113 1.36e-62 187
Msa1297130 MtrunA17_Chr7g0222991 67.164 134 43 1 1 134 10 142 3.00e-56 172
Msa1297130 MtrunA17_Chr7g0223001 60.150 133 39 3 3 134 27 146 2.33e-44 142
Msa1297130 MtrunA17_Chr5g0407311 63.793 116 39 2 1 116 1 113 1.21e-42 136
Msa1297130 MtrunA17_Chr7g0240791 96.970 66 2 0 1 66 1 66 1.38e-39 130
Msa1297130 MtrunA17_Chr8g0347231 56.122 98 42 1 37 133 28 125 3.66e-31 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1297130 AT4G40030.3 100.000 136 0 0 1 136 1 136 1.45e-96 274
Msa1297130 AT4G40030.1 100.000 136 0 0 1 136 1 136 1.45e-96 274
Msa1297130 AT4G40030.4 100.000 136 0 0 1 136 1 136 1.45e-96 274
Msa1297130 AT5G10980.1 100.000 136 0 0 1 136 1 136 1.45e-96 274
Msa1297130 AT4G40040.2 100.000 136 0 0 1 136 1 136 1.45e-96 274
Msa1297130 AT4G40040.3 100.000 136 0 0 1 136 1 136 1.45e-96 274
Msa1297130 AT4G40040.1 100.000 136 0 0 1 136 1 136 1.45e-96 274
Msa1297130 AT4G40030.2 100.000 136 0 0 1 136 29 164 2.12e-96 275
Msa1297130 AT1G09200.1 97.059 136 4 0 1 136 1 136 4.00e-93 265
Msa1297130 AT5G65360.1 97.059 136 4 0 1 136 1 136 4.00e-93 265
Msa1297130 AT5G10390.1 97.059 136 4 0 1 136 1 136 4.00e-93 265
Msa1297130 AT5G10400.1 97.059 136 4 0 1 136 1 136 4.00e-93 265
Msa1297130 AT3G27360.1 97.059 136 4 0 1 136 1 136 4.00e-93 265
Msa1297130 AT1G75600.1 96.324 136 5 0 1 136 1 136 1.06e-92 264
Msa1297130 AT1G13370.1 94.853 136 7 0 1 136 1 136 2.25e-91 261
Msa1297130 AT5G65350.1 92.647 136 10 0 1 136 1 136 1.32e-88 254
Msa1297130 AT1G19890.1 90.511 137 12 1 1 136 1 137 1.97e-85 246
Msa1297130 AT5G12910.1 71.111 135 34 2 1 135 1 130 1.19e-65 196
Msa1297130 AT1G01370.2 54.867 113 48 2 24 134 63 174 3.36e-32 112
Msa1297130 AT1G01370.1 54.867 113 48 2 24 134 63 174 3.36e-32 112
Msa1297130 AT1G01370.4 54.867 113 48 2 24 134 63 174 3.36e-32 112
Msa1297130 AT1G01370.3 54.867 113 48 2 24 134 63 174 3.36e-32 112

Find 60 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTCGTTAGCTGAAATT+TGG 0.181530 8_3:-57051843 None:intergenic
CCTAAGGAGGATGCTGCTTT+TGG 0.276400 8_3:+57053169 Msa1297130:exon
TGTGCATATTCGTTTGGAAT+TGG 0.300321 8_3:+57053442 Msa1297130:three_prime_UTR
AGCTTCTGTGCATATTCGTT+TGG 0.325215 8_3:+57053436 Msa1297130:three_prime_UTR
TTGGTTGCAAGTTGCTTCCT+TGG 0.333909 8_3:-57052466 None:intergenic
AACCGAAGAGCAACAGTTCC+TGG 0.350308 8_3:-57052649 None:intergenic
CCTTGCCAACTGGATGTCCT+TGG 0.351370 8_3:-57053127 None:intergenic
CACGGATCCTCCTTGCCAAC+TGG 0.363910 8_3:-57053137 None:intergenic
GTGCATATTCGTTTGGAATT+GGG 0.369903 8_3:+57053443 Msa1297130:three_prime_UTR
TCAAGAGCTCAGTACTCTTC+TGG 0.372688 8_3:-57052826 None:intergenic
GCTAGAAATACTAGATTAGA+TGG 0.379840 8_3:-57053416 None:intergenic
TAGCAAACAGTGCTTTAGAT+TGG 0.403208 8_3:-57053510 None:intergenic
TAATTTGCTTGCAATGACTT+TGG 0.419143 8_3:+57053321 Msa1297130:three_prime_UTR
CTTGCCAACTGGATGTCCTT+GGG 0.434839 8_3:-57053126 None:intergenic
ATATCTTGTTGGTCTCTTTG+AGG 0.438404 8_3:+57053059 Msa1297130:CDS
TGAAATTGCTCAGGACTTCA+AGG 0.441698 8_3:+57052880 Msa1297130:CDS
CAATTTCACGAACCAGTCTC+TGG 0.442239 8_3:-57052865 None:intergenic
CTTCCTTGGTGCCTTTCCAC+CGG 0.443477 8_3:-57052452 None:intergenic
TACTGGGCATGCAGCCACTC+TGG 0.448287 8_3:+57053365 Msa1297130:three_prime_UTR
TTCTTAACTCCACCAGTAGT+TGG 0.449824 8_3:-57052610 None:intergenic
GCCCAGGAACTGTTGCTCTT+CGG 0.449916 8_3:+57052647 Msa1297130:CDS
GCTGCTGAGGCATATCTTGT+TGG 0.457640 8_3:+57053048 Msa1297130:CDS
TGCATTGCAAGAGGCTGCTG+AGG 0.479818 8_3:+57053035 Msa1297130:CDS
AGAGTACTGAGCTCTTGATC+AGG 0.482557 8_3:+57052831 Msa1297130:CDS
CGTAAGTCTGCACCAACTAC+TGG 0.494282 8_3:+57052598 Msa1297130:CDS
ATCCGTGGTGAGCGCGCCTA+AGG 0.494321 8_3:+57053153 Msa1297130:CDS
GAAGCTCCCCTTCCAGAGAC+TGG 0.494411 8_3:+57052853 Msa1297130:CDS
ATGCAAGCACAGCATGACTC+TGG 0.500306 8_3:-57053017 None:intergenic
TTGAAATGATTTAGTTGCTA+GGG 0.505269 8_3:+57053230 Msa1297130:three_prime_UTR
CTAGATTAGATGGCAACAAA+AGG 0.506318 8_3:-57053406 None:intergenic
TGCTGTGCTTGCATTGCAAG+AGG 0.513898 8_3:+57053026 Msa1297130:CDS
CAAACTGCTCGTAAGTCAAC+CGG 0.517209 8_3:+57052433 Msa1297130:CDS
TGACTTACGAGCAGTTTGCT+TGG 0.520852 8_3:-57052428 None:intergenic
TTTGGTGCTGTTTAGATGGG+TGG 0.522117 8_3:+57053187 Msa1297130:three_prime_UTR
ACTGGTTCGTGAAATTGCTC+AGG 0.527548 8_3:+57052871 Msa1297130:CDS
ATTGAAATGATTTAGTTGCT+AGG 0.528912 8_3:+57053229 Msa1297130:three_prime_UTR
CACGAACCAGTCTCTGGAAG+GGG 0.535296 8_3:-57052859 None:intergenic
ATTCGTAGTTTGATTAATTG+CGG 0.539470 8_3:+57053262 Msa1297130:three_prime_UTR
CCAAAAGCAGCATCCTCCTT+AGG 0.547324 8_3:-57053169 None:intergenic
TAATAGTAACTCGCTTGGCA+TGG 0.547452 8_3:-57053101 None:intergenic
CTCCTTAGGCGCGCTCACCA+CGG 0.549887 8_3:-57053155 None:intergenic
ACCGAAGAGCAACAGTTCCT+GGG 0.564699 8_3:-57052648 None:intergenic
TTCACGAACCAGTCTCTGGA+AGG 0.566910 8_3:-57052861 None:intergenic
CGTGGTGAGCGCGCCTAAGG+AGG 0.568469 8_3:+57053156 Msa1297130:exon
TCGTAAGTCAACCGGTGGAA+AGG 0.572235 8_3:+57052441 Msa1297130:CDS
GGGCATAATAGTAACTCGCT+TGG 0.573260 8_3:-57053106 None:intergenic
AAGGAAGCAACTTGCAACCA+AGG 0.574800 8_3:+57052468 Msa1297130:CDS
CCAAGGACATCCAGTTGGCA+AGG 0.588868 8_3:+57053127 Msa1297130:CDS
AAACCACATCGTTATCGCCC+AGG 0.603518 8_3:+57052631 Msa1297130:CDS
AAGTCTGCACCAACTACTGG+TGG 0.611115 8_3:+57052601 Msa1297130:CDS
TCACGAACCAGTCTCTGGAA+GGG 0.612129 8_3:-57052860 None:intergenic
GCGAGTTACTATTATGCCCA+AGG 0.629580 8_3:+57053110 Msa1297130:CDS
TATGCCCAAGGACATCCAGT+TGG 0.656475 8_3:+57053122 Msa1297130:CDS
GTTCCTGGGCGATAACGATG+TGG 0.663160 8_3:-57052634 None:intergenic
CAGTTGGCAAGGAGGATCCG+TGG 0.664833 8_3:+57053138 Msa1297130:CDS
CAGCATGACTCTGGAAACGA+AGG 0.672290 8_3:-57053008 None:intergenic
AGGACATCCAGTTGGCAAGG+AGG 0.674393 8_3:+57053130 Msa1297130:CDS
ACTGCTCGTAAGTCAACCGG+TGG 0.674713 8_3:+57052436 Msa1297130:CDS
CCACAAAATATAATCCAGAG+TGG 0.681606 8_3:-57053379 None:intergenic
CAACCGGTGGAAAGGCACCA+AGG 0.683753 8_3:+57052449 Msa1297130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAAATCTATAGTAAATTA+GGG + chr8_3:57051909-57051928 Msa1297130:intron 15.0%
!!! ATTATGTTGCATTTTATATT+AGG + chr8_3:57052097-57052116 Msa1297130:intron 15.0%
!!! TCTGTTAAAAAATTTCTTTT+AGG + chr8_3:57052676-57052695 Msa1297130:intron 15.0%
!!! CTGTTAAAAAATTTCTTTTA+GGG + chr8_3:57052677-57052696 Msa1297130:intron 15.0%
!! TTCAAATCTATAGTAAATTA+GGG + chr8_3:57051909-57051928 Msa1297130:intron 15.0%
!!! ATTATGTTGCATTTTATATT+AGG + chr8_3:57052097-57052116 Msa1297130:intron 15.0%
!!! TCTGTTAAAAAATTTCTTTT+AGG + chr8_3:57052676-57052695 Msa1297130:intron 15.0%
!!! CTGTTAAAAAATTTCTTTTA+GGG + chr8_3:57052677-57052696 Msa1297130:intron 15.0%
!! AGAAAAAAGCAAAAATTTCA+AGG + chr8_3:57051874-57051893 Msa1297130:five_prime_UTR 20.0%
!! CTTCAAATCTATAGTAAATT+AGG + chr8_3:57051908-57051927 Msa1297130:intron 20.0%
!! AATAAACATTCGAATTAGAA+AGG - chr8_3:57052080-57052099 None:intergenic 20.0%
!!! CTTTTATCAAAATTTGTTTC+TGG + chr8_3:57052145-57052164 Msa1297130:intron 20.0%
!!! TCATGTTTGAATTTTGATTA+AGG + chr8_3:57052178-57052197 Msa1297130:intron 20.0%
!!! AATAAGATAAATTAGCCTAA+CGG - chr8_3:57052283-57052302 None:intergenic 20.0%
!!! GGCTAATTTATCTTATTTTT+AGG + chr8_3:57052286-57052305 Msa1297130:intron 20.0%
!! ATTGTTAATTGTTGAAGATT+AGG + chr8_3:57052326-57052345 Msa1297130:intron 20.0%
!!! AAGATTAGGTTTTAGTTATT+AGG + chr8_3:57052340-57052359 Msa1297130:intron 20.0%
!!! TTAGGTTAAACAATGATTTT+AGG + chr8_3:57052358-57052377 Msa1297130:intron 20.0%
!!! TAGGTTAAACAATGATTTTA+GGG + chr8_3:57052359-57052378 Msa1297130:intron 20.0%
!!! GTTTATAATGATTTGTCATT+TGG + chr8_3:57052770-57052789 Msa1297130:intron 20.0%
!!! TATTTTAAAACAGTGAGATT+AGG + chr8_3:57052798-57052817 Msa1297130:intron 20.0%
!!! GTTTCTAATAATTTTGGTTT+TGG + chr8_3:57052974-57052993 Msa1297130:intron 20.0%
!!! TATTCTGCTATTTTAGTTTT+TGG + chr8_3:57053294-57053313 Msa1297130:three_prime_UTR 20.0%
!!! ATTCTGCTATTTTAGTTTTT+GGG + chr8_3:57053295-57053314 Msa1297130:three_prime_UTR 20.0%
!!! TTATATGTTCTTGTTTTACT+GGG + chr8_3:57053349-57053368 Msa1297130:three_prime_UTR 20.0%
!! AGAAAAAAGCAAAAATTTCA+AGG + chr8_3:57051874-57051893 Msa1297130:five_prime_UTR 20.0%
!! CTTCAAATCTATAGTAAATT+AGG + chr8_3:57051908-57051927 Msa1297130:intron 20.0%
!! AATAAACATTCGAATTAGAA+AGG - chr8_3:57052080-57052099 None:intergenic 20.0%
!!! CTTTTATCAAAATTTGTTTC+TGG + chr8_3:57052145-57052164 Msa1297130:intron 20.0%
!!! TCATGTTTGAATTTTGATTA+AGG + chr8_3:57052178-57052197 Msa1297130:intron 20.0%
!!! AATAAGATAAATTAGCCTAA+CGG - chr8_3:57052283-57052302 None:intergenic 20.0%
!!! GGCTAATTTATCTTATTTTT+AGG + chr8_3:57052286-57052305 Msa1297130:intron 20.0%
!! ATTGTTAATTGTTGAAGATT+AGG + chr8_3:57052326-57052345 Msa1297130:intron 20.0%
!!! AAGATTAGGTTTTAGTTATT+AGG + chr8_3:57052340-57052359 Msa1297130:intron 20.0%
!!! TTAGGTTAAACAATGATTTT+AGG + chr8_3:57052358-57052377 Msa1297130:intron 20.0%
!!! TAGGTTAAACAATGATTTTA+GGG + chr8_3:57052359-57052378 Msa1297130:intron 20.0%
!!! GTTTATAATGATTTGTCATT+TGG + chr8_3:57052770-57052789 Msa1297130:intron 20.0%
!!! TATTTTAAAACAGTGAGATT+AGG + chr8_3:57052798-57052817 Msa1297130:intron 20.0%
!!! GTTTCTAATAATTTTGGTTT+TGG + chr8_3:57052974-57052993 Msa1297130:intron 20.0%
!!! TATTCTGCTATTTTAGTTTT+TGG + chr8_3:57053294-57053313 Msa1297130:three_prime_UTR 20.0%
!!! ATTCTGCTATTTTAGTTTTT+GGG + chr8_3:57053295-57053314 Msa1297130:three_prime_UTR 20.0%
!!! TTATATGTTCTTGTTTTACT+GGG + chr8_3:57053349-57053368 Msa1297130:three_prime_UTR 20.0%
! ATTCTCCAATTTACGAAATT+AGG + chr8_3:57052012-57052031 Msa1297130:intron 25.0%
! TTCTCCAATTTACGAAATTA+GGG + chr8_3:57052013-57052032 Msa1297130:intron 25.0%
! TAAACCCTAATTTCGTAAAT+TGG - chr8_3:57052020-57052039 None:intergenic 25.0%
!! AGGTTAAACAATGATTTTAG+GGG + chr8_3:57052360-57052379 Msa1297130:intron 25.0%
! ATTGTTCTGATTATCTTGTT+TGG + chr8_3:57052385-57052404 Msa1297130:intron 25.0%
! AATCAACTAAAATCAAACCT+TGG - chr8_3:57052488-57052507 None:intergenic 25.0%
!!! CTTGTTTTTCTCTTAACTTT+AGG + chr8_3:57052570-57052589 Msa1297130:intron 25.0%
!!! TTGTTTCAATTGAGTTGAAT+TGG + chr8_3:57052743-57052762 Msa1297130:intron 25.0%
!! TAATGATTTGTCATTTGGAT+TGG + chr8_3:57052775-57052794 Msa1297130:intron 25.0%
!! GTTGTGGTTTCTAATAATTT+TGG + chr8_3:57052968-57052987 Msa1297130:intron 25.0%
! ATTGAAATGATTTAGTTGCT+AGG + chr8_3:57053229-57053248 Msa1297130:three_prime_UTR 25.0%
! TTGAAATGATTTAGTTGCTA+GGG + chr8_3:57053230-57053249 Msa1297130:three_prime_UTR 25.0%
! ATTCGTAGTTTGATTAATTG+CGG + chr8_3:57053262-57053281 Msa1297130:three_prime_UTR 25.0%
!!! GTTATATGTTCTTGTTTTAC+TGG + chr8_3:57053348-57053367 Msa1297130:three_prime_UTR 25.0%
! ATTCTCCAATTTACGAAATT+AGG + chr8_3:57052012-57052031 Msa1297130:intron 25.0%
! TTCTCCAATTTACGAAATTA+GGG + chr8_3:57052013-57052032 Msa1297130:intron 25.0%
! TAAACCCTAATTTCGTAAAT+TGG - chr8_3:57052020-57052039 None:intergenic 25.0%
!! AGGTTAAACAATGATTTTAG+GGG + chr8_3:57052360-57052379 Msa1297130:intron 25.0%
! ATTGTTCTGATTATCTTGTT+TGG + chr8_3:57052385-57052404 Msa1297130:intron 25.0%
! AATCAACTAAAATCAAACCT+TGG - chr8_3:57052488-57052507 None:intergenic 25.0%
!!! CTTGTTTTTCTCTTAACTTT+AGG + chr8_3:57052570-57052589 Msa1297130:intron 25.0%
!!! TTGTTTCAATTGAGTTGAAT+TGG + chr8_3:57052743-57052762 Msa1297130:intron 25.0%
!! TAATGATTTGTCATTTGGAT+TGG + chr8_3:57052775-57052794 Msa1297130:intron 25.0%
!! GTTGTGGTTTCTAATAATTT+TGG + chr8_3:57052968-57052987 Msa1297130:intron 25.0%
! ATTGAAATGATTTAGTTGCT+AGG + chr8_3:57053229-57053248 Msa1297130:three_prime_UTR 25.0%
! TTGAAATGATTTAGTTGCTA+GGG + chr8_3:57053230-57053249 Msa1297130:three_prime_UTR 25.0%
! ATTCGTAGTTTGATTAATTG+CGG + chr8_3:57053262-57053281 Msa1297130:three_prime_UTR 25.0%
!!! GTTATATGTTCTTGTTTTAC+TGG + chr8_3:57053348-57053367 Msa1297130:three_prime_UTR 25.0%
GAAATTAGGGTTTACGAATT+CGG + chr8_3:57052026-57052045 Msa1297130:intron 30.0%
GTTCACAATCAGTTTAGTTA+AGG + chr8_3:57052946-57052965 Msa1297130:intron 30.0%
AATCAGTTTAGTTAAGGTTG+TGG + chr8_3:57052952-57052971 Msa1297130:intron 30.0%
!! TAATTTGCTTGCAATGACTT+TGG + chr8_3:57053321-57053340 Msa1297130:three_prime_UTR 30.0%
GCTAGAAATACTAGATTAGA+TGG - chr8_3:57053419-57053438 None:intergenic 30.0%
GAAATTAGGGTTTACGAATT+CGG + chr8_3:57052026-57052045 Msa1297130:intron 30.0%
GTTCACAATCAGTTTAGTTA+AGG + chr8_3:57052946-57052965 Msa1297130:intron 30.0%
AATCAGTTTAGTTAAGGTTG+TGG + chr8_3:57052952-57052971 Msa1297130:intron 30.0%
!! TAATTTGCTTGCAATGACTT+TGG + chr8_3:57053321-57053340 Msa1297130:three_prime_UTR 30.0%
GCTAGAAATACTAGATTAGA+TGG - chr8_3:57053419-57053438 None:intergenic 30.0%
! GTTTATAGCTTCTGTGCTTT+TGG + chr8_3:57051931-57051950 Msa1297130:intron 35.0%
! GTTACATTCATCCACGTTTT+AGG + chr8_3:57052234-57052253 Msa1297130:intron 35.0%
TTCTTCTCTTTCCTAAAACG+TGG - chr8_3:57052248-57052267 None:intergenic 35.0%
CTTGTTTGGATTTACGTAGA+TGG + chr8_3:57052399-57052418 Msa1297130:intron 35.0%
!! TCAATTGAGTTGAATTGGCT+TGG + chr8_3:57052748-57052767 Msa1297130:intron 35.0%
! ATATCTTGTTGGTCTCTTTG+AGG + chr8_3:57053059-57053078 Msa1297130:CDS 35.0%
CCACAAAATATAATCCAGAG+TGG - chr8_3:57053382-57053401 None:intergenic 35.0%
! CCACTCTGGATTATATTTTG+TGG + chr8_3:57053379-57053398 Msa1297130:three_prime_UTR 35.0%
CTAGATTAGATGGCAACAAA+AGG - chr8_3:57053409-57053428 None:intergenic 35.0%
! TGTGCATATTCGTTTGGAAT+TGG + chr8_3:57053442-57053461 Msa1297130:three_prime_UTR 35.0%
! GTGCATATTCGTTTGGAATT+GGG + chr8_3:57053443-57053462 Msa1297130:three_prime_UTR 35.0%
! TAGCAAACAGTGCTTTAGAT+TGG - chr8_3:57053513-57053532 None:intergenic 35.0%
! GTTTATAGCTTCTGTGCTTT+TGG + chr8_3:57051931-57051950 Msa1297130:intron 35.0%
! GTTACATTCATCCACGTTTT+AGG + chr8_3:57052234-57052253 Msa1297130:intron 35.0%
TTCTTCTCTTTCCTAAAACG+TGG - chr8_3:57052248-57052267 None:intergenic 35.0%
CTTGTTTGGATTTACGTAGA+TGG + chr8_3:57052399-57052418 Msa1297130:intron 35.0%
!! TCAATTGAGTTGAATTGGCT+TGG + chr8_3:57052748-57052767 Msa1297130:intron 35.0%
! ATATCTTGTTGGTCTCTTTG+AGG + chr8_3:57053059-57053078 Msa1297130:CDS 35.0%
CCACAAAATATAATCCAGAG+TGG - chr8_3:57053382-57053401 None:intergenic 35.0%
! CCACTCTGGATTATATTTTG+TGG + chr8_3:57053379-57053398 Msa1297130:three_prime_UTR 35.0%
CTAGATTAGATGGCAACAAA+AGG - chr8_3:57053409-57053428 None:intergenic 35.0%
! TGTGCATATTCGTTTGGAAT+TGG + chr8_3:57053442-57053461 Msa1297130:three_prime_UTR 35.0%
! GTGCATATTCGTTTGGAATT+GGG + chr8_3:57053443-57053462 Msa1297130:three_prime_UTR 35.0%
! TAGCAAACAGTGCTTTAGAT+TGG - chr8_3:57053513-57053532 None:intergenic 35.0%
ACGAATTCGGTAGATGTGTT+TGG + chr8_3:57052039-57052058 Msa1297130:intron 40.0%
TTCTTAACTCCACCAGTAGT+TGG - chr8_3:57052613-57052632 None:intergenic 40.0%
TGAAATTGCTCAGGACTTCA+AGG + chr8_3:57052880-57052899 Msa1297130:CDS 40.0%
TAATAGTAACTCGCTTGGCA+TGG - chr8_3:57053104-57053123 None:intergenic 40.0%
!!! TGCTTTTGGTGCTGTTTAGA+TGG + chr8_3:57053183-57053202 Msa1297130:three_prime_UTR 40.0%
!!! GCTTTTGGTGCTGTTTAGAT+GGG + chr8_3:57053184-57053203 Msa1297130:three_prime_UTR 40.0%
AGCTTCTGTGCATATTCGTT+TGG + chr8_3:57053436-57053455 Msa1297130:three_prime_UTR 40.0%
ACGAATTCGGTAGATGTGTT+TGG + chr8_3:57052039-57052058 Msa1297130:intron 40.0%
TTCTTAACTCCACCAGTAGT+TGG - chr8_3:57052613-57052632 None:intergenic 40.0%
TGAAATTGCTCAGGACTTCA+AGG + chr8_3:57052880-57052899 Msa1297130:CDS 40.0%
TAATAGTAACTCGCTTGGCA+TGG - chr8_3:57053104-57053123 None:intergenic 40.0%
!!! TGCTTTTGGTGCTGTTTAGA+TGG + chr8_3:57053183-57053202 Msa1297130:three_prime_UTR 40.0%
!!! GCTTTTGGTGCTGTTTAGAT+GGG + chr8_3:57053184-57053203 Msa1297130:three_prime_UTR 40.0%
AGCTTCTGTGCATATTCGTT+TGG + chr8_3:57053436-57053455 Msa1297130:three_prime_UTR 40.0%
GAAGCAATTGAAGCTCCGTT+AGG + chr8_3:57052265-57052284 Msa1297130:intron 45.0%
! TGACTTACGAGCAGTTTGCT+TGG - chr8_3:57052431-57052450 None:intergenic 45.0%
CAAACTGCTCGTAAGTCAAC+CGG + chr8_3:57052433-57052452 Msa1297130:CDS 45.0%
TTGGTTGCAAGTTGCTTCCT+TGG - chr8_3:57052469-57052488 None:intergenic 45.0%
AAGGAAGCAACTTGCAACCA+AGG + chr8_3:57052468-57052487 Msa1297130:CDS 45.0%
TCAAGAGCTCAGTACTCTTC+TGG - chr8_3:57052829-57052848 None:intergenic 45.0%
!! AGAGTACTGAGCTCTTGATC+AGG + chr8_3:57052831-57052850 Msa1297130:CDS 45.0%
CAATTTCACGAACCAGTCTC+TGG - chr8_3:57052868-57052887 None:intergenic 45.0%
!! ACTGGTTCGTGAAATTGCTC+AGG + chr8_3:57052871-57052890 Msa1297130:CDS 45.0%
GGGCATAATAGTAACTCGCT+TGG - chr8_3:57053109-57053128 None:intergenic 45.0%
GCGAGTTACTATTATGCCCA+AGG + chr8_3:57053110-57053129 Msa1297130:CDS 45.0%
!! TTTGGTGCTGTTTAGATGGG+TGG + chr8_3:57053187-57053206 Msa1297130:three_prime_UTR 45.0%
GAAGCAATTGAAGCTCCGTT+AGG + chr8_3:57052265-57052284 Msa1297130:intron 45.0%
! TGACTTACGAGCAGTTTGCT+TGG - chr8_3:57052431-57052450 None:intergenic 45.0%
CAAACTGCTCGTAAGTCAAC+CGG + chr8_3:57052433-57052452 Msa1297130:CDS 45.0%
TTGGTTGCAAGTTGCTTCCT+TGG - chr8_3:57052469-57052488 None:intergenic 45.0%
AAGGAAGCAACTTGCAACCA+AGG + chr8_3:57052468-57052487 Msa1297130:CDS 45.0%
TCAAGAGCTCAGTACTCTTC+TGG - chr8_3:57052829-57052848 None:intergenic 45.0%
!! AGAGTACTGAGCTCTTGATC+AGG + chr8_3:57052831-57052850 Msa1297130:CDS 45.0%
CAATTTCACGAACCAGTCTC+TGG - chr8_3:57052868-57052887 None:intergenic 45.0%
!! ACTGGTTCGTGAAATTGCTC+AGG + chr8_3:57052871-57052890 Msa1297130:CDS 45.0%
GGGCATAATAGTAACTCGCT+TGG - chr8_3:57053109-57053128 None:intergenic 45.0%
GCGAGTTACTATTATGCCCA+AGG + chr8_3:57053110-57053129 Msa1297130:CDS 45.0%
!! TTTGGTGCTGTTTAGATGGG+TGG + chr8_3:57053187-57053206 Msa1297130:three_prime_UTR 45.0%
TCGTAAGTCAACCGGTGGAA+AGG + chr8_3:57052441-57052460 Msa1297130:CDS 50.0%
CGTAAGTCTGCACCAACTAC+TGG + chr8_3:57052598-57052617 Msa1297130:CDS 50.0%
AAGTCTGCACCAACTACTGG+TGG + chr8_3:57052601-57052620 Msa1297130:CDS 50.0%
AAACCACATCGTTATCGCCC+AGG + chr8_3:57052631-57052650 Msa1297130:CDS 50.0%
ACCGAAGAGCAACAGTTCCT+GGG - chr8_3:57052651-57052670 None:intergenic 50.0%
AACCGAAGAGCAACAGTTCC+TGG - chr8_3:57052652-57052671 None:intergenic 50.0%
! TCACGAACCAGTCTCTGGAA+GGG - chr8_3:57052863-57052882 None:intergenic 50.0%
TTCACGAACCAGTCTCTGGA+AGG - chr8_3:57052864-57052883 None:intergenic 50.0%
! CAGCATGACTCTGGAAACGA+AGG - chr8_3:57053011-57053030 None:intergenic 50.0%
ATGCAAGCACAGCATGACTC+TGG - chr8_3:57053020-57053039 None:intergenic 50.0%
TGCTGTGCTTGCATTGCAAG+AGG + chr8_3:57053026-57053045 Msa1297130:CDS 50.0%
GCTGCTGAGGCATATCTTGT+TGG + chr8_3:57053048-57053067 Msa1297130:CDS 50.0%
TATGCCCAAGGACATCCAGT+TGG + chr8_3:57053122-57053141 Msa1297130:CDS 50.0%
CTTGCCAACTGGATGTCCTT+GGG - chr8_3:57053129-57053148 None:intergenic 50.0%
CCAAAAGCAGCATCCTCCTT+AGG - chr8_3:57053172-57053191 None:intergenic 50.0%
!!! CCTAAGGAGGATGCTGCTTT+TGG + chr8_3:57053169-57053188 Msa1297130:exon 50.0%
TCGTAAGTCAACCGGTGGAA+AGG + chr8_3:57052441-57052460 Msa1297130:CDS 50.0%
CGTAAGTCTGCACCAACTAC+TGG + chr8_3:57052598-57052617 Msa1297130:CDS 50.0%
AAGTCTGCACCAACTACTGG+TGG + chr8_3:57052601-57052620 Msa1297130:CDS 50.0%
AAACCACATCGTTATCGCCC+AGG + chr8_3:57052631-57052650 Msa1297130:CDS 50.0%
ACCGAAGAGCAACAGTTCCT+GGG - chr8_3:57052651-57052670 None:intergenic 50.0%
AACCGAAGAGCAACAGTTCC+TGG - chr8_3:57052652-57052671 None:intergenic 50.0%
! TCACGAACCAGTCTCTGGAA+GGG - chr8_3:57052863-57052882 None:intergenic 50.0%
TTCACGAACCAGTCTCTGGA+AGG - chr8_3:57052864-57052883 None:intergenic 50.0%
! CAGCATGACTCTGGAAACGA+AGG - chr8_3:57053011-57053030 None:intergenic 50.0%
ATGCAAGCACAGCATGACTC+TGG - chr8_3:57053020-57053039 None:intergenic 50.0%
TGCTGTGCTTGCATTGCAAG+AGG + chr8_3:57053026-57053045 Msa1297130:CDS 50.0%
GCTGCTGAGGCATATCTTGT+TGG + chr8_3:57053048-57053067 Msa1297130:CDS 50.0%
TATGCCCAAGGACATCCAGT+TGG + chr8_3:57053122-57053141 Msa1297130:CDS 50.0%
CTTGCCAACTGGATGTCCTT+GGG - chr8_3:57053129-57053148 None:intergenic 50.0%
CCAAAAGCAGCATCCTCCTT+AGG - chr8_3:57053172-57053191 None:intergenic 50.0%
!!! CCTAAGGAGGATGCTGCTTT+TGG + chr8_3:57053169-57053188 Msa1297130:exon 50.0%
ACTGCTCGTAAGTCAACCGG+TGG + chr8_3:57052436-57052455 Msa1297130:CDS 55.0%
!! CTTCCTTGGTGCCTTTCCAC+CGG - chr8_3:57052455-57052474 None:intergenic 55.0%
GTTCCTGGGCGATAACGATG+TGG - chr8_3:57052637-57052656 None:intergenic 55.0%
GCCCAGGAACTGTTGCTCTT+CGG + chr8_3:57052647-57052666 Msa1297130:CDS 55.0%
! CACGAACCAGTCTCTGGAAG+GGG - chr8_3:57052862-57052881 None:intergenic 55.0%
TGCATTGCAAGAGGCTGCTG+AGG + chr8_3:57053035-57053054 Msa1297130:CDS 55.0%
CCTTGCCAACTGGATGTCCT+TGG - chr8_3:57053130-57053149 None:intergenic 55.0%
CCAAGGACATCCAGTTGGCA+AGG + chr8_3:57053127-57053146 Msa1297130:CDS 55.0%
AGGACATCCAGTTGGCAAGG+AGG + chr8_3:57053130-57053149 Msa1297130:CDS 55.0%
ACTGCTCGTAAGTCAACCGG+TGG + chr8_3:57052436-57052455 Msa1297130:CDS 55.0%
!! CTTCCTTGGTGCCTTTCCAC+CGG - chr8_3:57052455-57052474 None:intergenic 55.0%
GTTCCTGGGCGATAACGATG+TGG - chr8_3:57052637-57052656 None:intergenic 55.0%
GCCCAGGAACTGTTGCTCTT+CGG + chr8_3:57052647-57052666 Msa1297130:CDS 55.0%
! CACGAACCAGTCTCTGGAAG+GGG - chr8_3:57052862-57052881 None:intergenic 55.0%
TGCATTGCAAGAGGCTGCTG+AGG + chr8_3:57053035-57053054 Msa1297130:CDS 55.0%
CCTTGCCAACTGGATGTCCT+TGG - chr8_3:57053130-57053149 None:intergenic 55.0%
CCAAGGACATCCAGTTGGCA+AGG + chr8_3:57053127-57053146 Msa1297130:CDS 55.0%
AGGACATCCAGTTGGCAAGG+AGG + chr8_3:57053130-57053149 Msa1297130:CDS 55.0%
!! CAACCGGTGGAAAGGCACCA+AGG + chr8_3:57052449-57052468 Msa1297130:CDS 60.0%
GAAGCTCCCCTTCCAGAGAC+TGG + chr8_3:57052853-57052872 Msa1297130:CDS 60.0%
CACGGATCCTCCTTGCCAAC+TGG - chr8_3:57053140-57053159 None:intergenic 60.0%
CAGTTGGCAAGGAGGATCCG+TGG + chr8_3:57053138-57053157 Msa1297130:CDS 60.0%
TACTGGGCATGCAGCCACTC+TGG + chr8_3:57053365-57053384 Msa1297130:three_prime_UTR 60.0%
!! CAACCGGTGGAAAGGCACCA+AGG + chr8_3:57052449-57052468 Msa1297130:CDS 60.0%
GAAGCTCCCCTTCCAGAGAC+TGG + chr8_3:57052853-57052872 Msa1297130:CDS 60.0%
CACGGATCCTCCTTGCCAAC+TGG - chr8_3:57053140-57053159 None:intergenic 60.0%
CAGTTGGCAAGGAGGATCCG+TGG + chr8_3:57053138-57053157 Msa1297130:CDS 60.0%
TACTGGGCATGCAGCCACTC+TGG + chr8_3:57053365-57053384 Msa1297130:three_prime_UTR 60.0%
ATCCGTGGTGAGCGCGCCTA+AGG + chr8_3:57053153-57053172 Msa1297130:CDS 65.0%
CTCCTTAGGCGCGCTCACCA+CGG - chr8_3:57053158-57053177 None:intergenic 65.0%
ATCCGTGGTGAGCGCGCCTA+AGG + chr8_3:57053153-57053172 Msa1297130:CDS 65.0%
CTCCTTAGGCGCGCTCACCA+CGG - chr8_3:57053158-57053177 None:intergenic 65.0%
CGTGGTGAGCGCGCCTAAGG+AGG + chr8_3:57053156-57053175 Msa1297130:exon 70.0%
CGTGGTGAGCGCGCCTAAGG+AGG + chr8_3:57053156-57053175 Msa1297130:exon 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 57051845 57053540 57051845 ID=Msa1297130;Name=Msa1297130
chr8_3 mRNA 57051845 57053540 57051845 ID=Msa1297130-mRNA-1;Parent=Msa1297130;Name=Msa1297130-mRNA-1;_AED=0.31;_eAED=0.31;_QI=51|1|1|1|0.75|0.8|5|367|136
chr8_3 exon 57051845 57051895 57051845 ID=Msa1297130-mRNA-1:exon:16363;Parent=Msa1297130-mRNA-1
chr8_3 exon 57052418 57052489 57052418 ID=Msa1297130-mRNA-1:exon:16364;Parent=Msa1297130-mRNA-1
chr8_3 exon 57052592 57052668 57052592 ID=Msa1297130-mRNA-1:exon:16365;Parent=Msa1297130-mRNA-1
chr8_3 exon 57052811 57052901 57052811 ID=Msa1297130-mRNA-1:exon:16366;Parent=Msa1297130-mRNA-1
chr8_3 exon 57053003 57053540 57053003 ID=Msa1297130-mRNA-1:exon:16367;Parent=Msa1297130-mRNA-1
chr8_3 five_prime_UTR 57051845 57051895 57051845 ID=Msa1297130-mRNA-1:five_prime_utr;Parent=Msa1297130-mRNA-1
chr8_3 CDS 57052418 57052489 57052418 ID=Msa1297130-mRNA-1:cds;Parent=Msa1297130-mRNA-1
chr8_3 CDS 57052592 57052668 57052592 ID=Msa1297130-mRNA-1:cds;Parent=Msa1297130-mRNA-1
chr8_3 CDS 57052811 57052901 57052811 ID=Msa1297130-mRNA-1:cds;Parent=Msa1297130-mRNA-1
chr8_3 CDS 57053003 57053173 57053003 ID=Msa1297130-mRNA-1:cds;Parent=Msa1297130-mRNA-1
chr8_3 three_prime_UTR 57053174 57053540 57053174 ID=Msa1297130-mRNA-1:three_prime_utr;Parent=Msa1297130-mRNA-1
Gene Sequence

>Msa1297130

ATGGCACGTACCAAGCAAACTGCTCGTAAGTCAACCGGTGGAAAGGCACCAAGGAAGCAACTTGCAACCAAGGCTGCACGTAAGTCTGCACCAACTACTGGTGGAGTTAAGAAACCACATCGTTATCGCCCAGGAACTGTTGCTCTTCGTGAGATTAGGAAGTACCAGAAGAGTACTGAGCTCTTGATCAGGAAGCTCCCCTTCCAGAGACTGGTTCGTGAAATTGCTCAGGACTTCAAGACTGACCTTCGTTTCCAGAGTCATGCTGTGCTTGCATTGCAAGAGGCTGCTGAGGCATATCTTGTTGGTCTCTTTGAGGACACTAACTTGTGTGCTATCCATGCCAAGCGAGTTACTATTATGCCCAAGGACATCCAGTTGGCAAGGAGGATCCGTGGTGAGCGCGCCTAA
Protein sequence

>Msa1297130

MARTKQTARKSTGGKAPRKQLATKAARKSAPTTGGVKKPHRYRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSHAVLALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA*