AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1298660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1298660 MtrunA17_Chr4g0058911 99.507 203 1 0 1 203 1 203 9.22e-150 413
Msa1298660 MtrunA17_Chr4g0058921 52.113 71 34 0 99 169 13 83 2.51e-16 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1298660 AT5G63000.1 69.307 202 61 1 1 202 1 201 2.37e-99 286

Find 83 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTACCTGGTATGGTTAAT+TGG 0.114650 8_3:+59479673 Msa1298660:CDS
AAGGAAATCAATGCACTTTA+AGG 0.161030 8_3:+59482363 Msa1298660:three_prime_UTR
TCAGCCACTGCTTCAGCTTT+TGG 0.199008 8_3:+59481340 Msa1298660:CDS
CATTGATTTCCTTCTCGTTT+TGG 0.231192 8_3:-59482353 None:intergenic
TGAAAGAAGCTTTGTGTAAC+AGG 0.261776 8_3:+59482391 Msa1298660:three_prime_UTR
CACCGAATGGAAATGAGATT+TGG 0.323929 8_3:-59479451 None:intergenic
ATTACCTGGTATGGTTAATT+GGG 0.333661 8_3:+59479674 Msa1298660:CDS
GTATTTGCAGGCATGTTATA+TGG 0.355049 8_3:+59479717 Msa1298660:CDS
AATAGCTAATGAAGAGAATC+TGG 0.374032 8_3:+59481944 Msa1298660:CDS
CCGTTGGCGAGCAAGGAATA+TGG 0.376757 8_3:+59481625 Msa1298660:CDS
ATGAACATCCTCCTCTTCAT+AGG 0.397466 8_3:+59482167 Msa1298660:three_prime_UTR
GCTCGCCAACGGAGCGATCC+TGG 0.400080 8_3:-59481614 None:intergenic
ATATAACATGCCTGCAAATA+CGG 0.405017 8_3:-59479715 None:intergenic
ACTGTTATTTCCGTATTTGC+AGG 0.406477 8_3:+59479705 Msa1298660:CDS
AATGAAAGAAAGCTGTTGTT+AGG 0.410201 8_3:+59482045 Msa1298660:exon
CTATTTCTACCATCTTCAAA+TGG 0.430805 8_3:-59481926 None:intergenic
TAATTCAGATATTTAGCGTC+AGG 0.449664 8_3:+59482201 Msa1298660:three_prime_UTR
AGGAAATCAATGCACTTTAA+GGG 0.458893 8_3:+59482364 Msa1298660:three_prime_UTR
TTCAGATCAGAGAGAAGTAA+AGG 0.462142 8_3:+59481974 Msa1298660:CDS
TTCTCAAAGATTACCTGGTA+TGG 0.463601 8_3:+59479665 Msa1298660:CDS
AGAAAATGATTCGGTGGGTA+TGG 0.464366 8_3:-59479405 None:intergenic
GCATGTTATATGGTGGTAGC+AGG 0.465871 8_3:+59479727 Msa1298660:CDS
TGATTCGGTGGGTATGGTGG+TGG 0.466420 8_3:-59479399 None:intergenic
TCAGTAGCTTCCATTGTAAG+AGG 0.473319 8_3:-59479486 None:intergenic
AATGGAAATGAGATTTGGAT+AGG 0.477123 8_3:-59479446 None:intergenic
ATCCAAATCTCATTTCCATT+CGG 0.490859 8_3:+59479449 Msa1298660:five_prime_UTR
CAAATCTCATTTCCATTCGG+TGG 0.493374 8_3:+59479452 Msa1298660:five_prime_UTR
GTTACACGAGGCGCACTAGT+TGG 0.497149 8_3:+59481214 Msa1298660:CDS
CAGGATTCTCAAAGATTACC+TGG 0.503713 8_3:+59479660 Msa1298660:intron
CTAGTTGGAGGGGTACGACT+TGG 0.505189 8_3:+59481229 Msa1298660:CDS
ATCGATCTAAGAAAATGATT+CGG 0.505355 8_3:-59479414 None:intergenic
TATGGCACTAGGATCAGTAC+TGG 0.511165 8_3:+59481643 Msa1298660:CDS
TCAGATCAGAGAGAAGTAAA+GGG 0.520907 8_3:+59481975 Msa1298660:CDS
GATCGCTCCGTTGGCGAGCA+AGG 0.521665 8_3:+59481618 Msa1298660:CDS
ATGAGATTTGGATAGGGAAG+GGG 0.531733 8_3:-59479439 None:intergenic
ACACCTATGCTTAGCAGCAC+GGG 0.532536 8_3:+59482522 Msa1298660:three_prime_UTR
AGGTTGGATCCATTTGAAGA+TGG 0.536542 8_3:+59481917 Msa1298660:intron
TTTGATTTCGTATATTCTTG+GGG 0.537822 8_3:+59482323 Msa1298660:three_prime_UTR
GATTCGGTGGGTATGGTGGT+GGG 0.540277 8_3:-59479398 None:intergenic
ATGGAAATGAGATTTGGATA+GGG 0.541967 8_3:-59479445 None:intergenic
GATCTAAGAAAATGATTCGG+TGG 0.543122 8_3:-59479411 None:intergenic
GGGTATGGTGGTGGGCGCCG+TGG 0.544145 8_3:-59479390 None:intergenic
CAGCCCGTGCTGCTAAGCAT+AGG 0.548077 8_3:-59482525 None:intergenic
TGAGAGATGCAATGGAGAAA+CGG 0.551090 8_3:+59481182 Msa1298660:CDS
AAATGATTCGGTGGGTATGG+TGG 0.553119 8_3:-59479402 None:intergenic
GCAGAAGTGATGTTGAAACT+TGG 0.560534 8_3:+59481124 Msa1298660:CDS
CGAGCAAGGAATATGGCACT+AGG 0.560979 8_3:+59481632 Msa1298660:CDS
CGTACTAGCAAAATAACAGA+TGG 0.562257 8_3:-59482247 None:intergenic
CACACCTATGCTTAGCAGCA+CGG 0.562574 8_3:+59482521 Msa1298660:three_prime_UTR
TCGATTGATGAGAGATGCAA+TGG 0.565773 8_3:+59481174 Msa1298660:CDS
AATGAGATTTGGATAGGGAA+GGG 0.570960 8_3:-59479440 None:intergenic
CACGAGGCGCACTAGTTGGA+GGG 0.577186 8_3:+59481218 Msa1298660:CDS
CCATATTCCTTGCTCGCCAA+CGG 0.583219 8_3:-59481625 None:intergenic
CAGAAGTGATGTTGAAACTT+GGG 0.586058 8_3:+59481125 Msa1298660:CDS
GGTCCCCAATTAACCATACC+AGG 0.586682 8_3:-59479678 None:intergenic
TAATTATGTGTTGGACAATG+AGG 0.586924 8_3:+59482121 Msa1298660:three_prime_UTR
CGTGGAATCTTTCTCAGTCA+CGG 0.587382 8_3:-59479372 None:intergenic
AAATGAGATTTGGATAGGGA+AGG 0.590902 8_3:-59479441 None:intergenic
GGAATTATGTACATTTGAGA+CGG 0.591435 8_3:-59479526 None:intergenic
ATCTAAGAAAATGATTCGGT+GGG 0.591503 8_3:-59479410 None:intergenic
GCAAATACGGAAATAACAGT+TGG 0.592267 8_3:-59479702 None:intergenic
AAGAATAAACGTAAATGCAG+AGG 0.592391 8_3:+59482436 Msa1298660:three_prime_UTR
ACACGAGGCGCACTAGTTGG+AGG 0.595948 8_3:+59481217 Msa1298660:CDS
AATACGGAAATAACAGTTGG+TGG 0.602165 8_3:-59479699 None:intergenic
CATGTTATATGGTGGTAGCA+GGG 0.604326 8_3:+59479728 Msa1298660:CDS
ATTATGTACATTTGAGACGG+TGG 0.605444 8_3:-59479523 None:intergenic
GGAAGCTACTGACAACTCAT+CGG 0.605791 8_3:+59479497 Msa1298660:CDS
GCGCGGTAAAATCCACCGAA+TGG 0.606589 8_3:-59479464 None:intergenic
GGCTCACAGCCAAAACGAGA+AGG 0.614398 8_3:+59482344 Msa1298660:three_prime_UTR
ACAGGTGTGCTTGAAAGAGC+TGG 0.617068 8_3:+59482409 Msa1298660:three_prime_UTR
TAACATACAAAATCTGCTTG+CGG 0.620028 8_3:+59481273 Msa1298660:CDS
TTACCTGGTATGGTTAATTG+GGG 0.621137 8_3:+59479675 Msa1298660:CDS
TTTGCAGGCATGTTATATGG+TGG 0.635457 8_3:+59479720 Msa1298660:CDS
TAAACCAAAAGCTGAAGCAG+TGG 0.636557 8_3:-59481344 None:intergenic
TAGTGCCAGGATCGCTCCGT+TGG 0.642464 8_3:+59481609 Msa1298660:intron
AGCTTCCATTGTAAGAGGCG+CGG 0.647075 8_3:-59479481 None:intergenic
CGTGACTGAGAAAGATTCCA+CGG 0.662427 8_3:+59479373 Msa1298660:five_prime_UTR
CGGTTCATTCGAGTTACACG+AGG 0.671886 8_3:+59481202 Msa1298660:CDS
AATCTGCTTGCGGAGAAGCG+AGG 0.677314 8_3:+59481283 Msa1298660:CDS
ATGGCACTAGGATCAGTACT+GGG 0.690462 8_3:+59481644 Msa1298660:CDS
GATCAGAGAGAAGTAAAGGG+TGG 0.692664 8_3:+59481978 Msa1298660:CDS
ACGAGGCGCACTAGTTGGAG+GGG 0.697375 8_3:+59481219 Msa1298660:CDS
GTTATATGGTGGTAGCAGGG+AGG 0.722746 8_3:+59479731 Msa1298660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTATAGATATACTTTTTTA+AGG + chr8_3:59480113-59480132 Msa1298660:intron 15.0%
!! ATAAAAATAAAACTTAACAC+AGG - chr8_3:59480727-59480746 None:intergenic 15.0%
!! AATGTTGATATAAACATAAT+TGG - chr8_3:59481035-59481054 None:intergenic 15.0%
!! TGAGAAAATAAAAATGATAA+GGG - chr8_3:59482091-59482110 None:intergenic 15.0%
!! AAAAAAATTCCTCAAAGAAA+AGG + chr8_3:59479318-59479337 Msa1298660:five_prime_UTR 20.0%
!! AAAAAATTCCTCAAAGAAAA+GGG + chr8_3:59479319-59479338 Msa1298660:five_prime_UTR 20.0%
!! TTTCTTACTAATAAATACAC+AGG - chr8_3:59479827-59479846 None:intergenic 20.0%
!! TTATTAGTAAGAAATCTCAT+AGG + chr8_3:59479833-59479852 Msa1298660:intron 20.0%
!! AAGAAATAGCTTATAATAAG+TGG - chr8_3:59479901-59479920 None:intergenic 20.0%
!!! ATTTGACTTTAATTTATAGC+AGG - chr8_3:59479927-59479946 None:intergenic 20.0%
!!! TTTAGTTTTTTTTGTGAGTT+TGG + chr8_3:59480152-59480171 Msa1298660:intron 20.0%
!! TTACTTCTTATGTAATAAAG+AGG - chr8_3:59480262-59480281 None:intergenic 20.0%
!!! TTCTTTAGGTTGAAATATAA+AGG + chr8_3:59481531-59481550 Msa1298660:intron 20.0%
!!! TCTTTAGGTTGAAATATAAA+GGG + chr8_3:59481532-59481551 Msa1298660:intron 20.0%
!!! TTTTATTAAATTCTCACAAC+AGG - chr8_3:59481742-59481761 None:intergenic 20.0%
!!! TGTGACTATTTACTAAATTT+TGG + chr8_3:59481873-59481892 Msa1298660:intron 20.0%
!! GAGAAAATAAAAATGATAAG+GGG - chr8_3:59482090-59482109 None:intergenic 20.0%
!! CTGAGAAAATAAAAATGATA+AGG - chr8_3:59482092-59482111 None:intergenic 20.0%
!!! ATTTTTATTTTCTCAGAGAA+GGG + chr8_3:59482096-59482115 Msa1298660:three_prime_UTR 20.0%
!!! TTTTTGATTTCGTATATTCT+TGG + chr8_3:59482321-59482340 Msa1298660:three_prime_UTR 20.0%
!!! TTTTGATTTCGTATATTCTT+GGG + chr8_3:59482322-59482341 Msa1298660:three_prime_UTR 20.0%
!!! ATTTTTTTCCCTTTTCTTTG+AGG - chr8_3:59479330-59479349 None:intergenic 25.0%
! AAAAAAATAACTACAACGCT+CGG + chr8_3:59479342-59479361 Msa1298660:five_prime_UTR 25.0%
! ATCGATCTAAGAAAATGATT+CGG - chr8_3:59479417-59479436 None:intergenic 25.0%
! GCGAATTGAATGAATAAATA+AGG - chr8_3:59479550-59479569 None:intergenic 25.0%
! TTCTAATACTTTCGTTAATC+AGG + chr8_3:59479641-59479660 Msa1298660:intron 25.0%
!! TGCTATTGAAATCATAGTTT+TGG - chr8_3:59479792-59479811 None:intergenic 25.0%
!! ATTAAGTTGTTTAACCAAAC+AGG - chr8_3:59479860-59479879 None:intergenic 25.0%
! AAACACTTGTGATATTGTTT+GGG - chr8_3:59479989-59480008 None:intergenic 25.0%
! TAAACACTTGTGATATTGTT+TGG - chr8_3:59479990-59480009 None:intergenic 25.0%
! AAATTCTCAACAAGAAACAA+GGG - chr8_3:59480389-59480408 None:intergenic 25.0%
! AAAATTCTCAACAAGAAACA+AGG - chr8_3:59480390-59480409 None:intergenic 25.0%
! AACTAGTAATGTAAGAAAGT+AGG + chr8_3:59480414-59480433 Msa1298660:intron 25.0%
! AAATCATCTGTTAAAGGATT+TGG - chr8_3:59480490-59480509 None:intergenic 25.0%
! AAATCCTTTAACAGATGATT+TGG + chr8_3:59480489-59480508 Msa1298660:intron 25.0%
! ATCTAAACTAATTAAGACAC+AGG + chr8_3:59480540-59480559 Msa1298660:intron 25.0%
! AGCAATTTAAAGAAAAGAAC+CGG - chr8_3:59480661-59480680 None:intergenic 25.0%
! GAAGATCTATAACACTTATA+CGG - chr8_3:59480697-59480716 None:intergenic 25.0%
! ATGTAAACATATGTATGTGA+AGG - chr8_3:59480958-59480977 None:intergenic 25.0%
! TTGATTATACATTCTGTTTC+TGG + chr8_3:59481009-59481028 Msa1298660:intron 25.0%
! TTCACAAGATAGAGAAAAAA+GGG - chr8_3:59481507-59481526 None:intergenic 25.0%
! TTTCACAAGATAGAGAAAAA+AGG - chr8_3:59481508-59481527 None:intergenic 25.0%
!!! TTTTTTCTCTATCTTGTGAA+AGG + chr8_3:59481507-59481526 Msa1298660:intron 25.0%
!!! CTTTAGGTTGAAATATAAAG+GGG + chr8_3:59481533-59481552 Msa1298660:intron 25.0%
! TGTCTTTGTTAATCACTAAT+AGG + chr8_3:59481709-59481728 Msa1298660:intron 25.0%
! TGTCCTATTAAGTAAGTAAA+AGG + chr8_3:59481797-59481816 Msa1298660:intron 25.0%
!! ACTCCTTTTACTTACTTAAT+AGG - chr8_3:59481803-59481822 None:intergenic 25.0%
! TATTAAGTAAGTAAAAGGAG+TGG + chr8_3:59481802-59481821 Msa1298660:intron 25.0%
!! TTTAACTTATGTGTGCATAA+TGG + chr8_3:59481847-59481866 Msa1298660:intron 25.0%
!!! CATTTTTATTTTCTCAGAGA+AGG + chr8_3:59482095-59482114 Msa1298660:three_prime_UTR 25.0%
!!! TTTTTATTTTCTCAGAGAAG+GGG + chr8_3:59482097-59482116 Msa1298660:three_prime_UTR 25.0%
!! TTTGATTTCGTATATTCTTG+GGG + chr8_3:59482323-59482342 Msa1298660:three_prime_UTR 25.0%
AAAAAATAACTACAACGCTC+GGG + chr8_3:59479343-59479362 Msa1298660:five_prime_UTR 30.0%
!! ATCTAAGAAAATGATTCGGT+GGG - chr8_3:59479413-59479432 None:intergenic 30.0%
ATGGAAATGAGATTTGGATA+GGG - chr8_3:59479448-59479467 None:intergenic 30.0%
AATGGAAATGAGATTTGGAT+AGG - chr8_3:59479449-59479468 None:intergenic 30.0%
ATCCAAATCTCATTTCCATT+CGG + chr8_3:59479449-59479468 Msa1298660:five_prime_UTR 30.0%
GGAATTATGTACATTTGAGA+CGG - chr8_3:59479529-59479548 None:intergenic 30.0%
ATTACCTGGTATGGTTAATT+GGG + chr8_3:59479674-59479693 Msa1298660:CDS 30.0%
ATATAACATGCCTGCAAATA+CGG - chr8_3:59479718-59479737 None:intergenic 30.0%
!!! AATCATAGTTTTGGAGCATA+AGG - chr8_3:59479783-59479802 None:intergenic 30.0%
! TGTTTTCATGTAAGTTATCC+TGG + chr8_3:59479947-59479966 Msa1298660:intron 30.0%
ATAGTGTATTATGTTCAACG+TGG + chr8_3:59480051-59480070 Msa1298660:intron 30.0%
TAGTGTTAAACTTCTCATCA+TGG - chr8_3:59480204-59480223 None:intergenic 30.0%
ATGATGAGAAGTTTAACACT+AGG + chr8_3:59480203-59480222 Msa1298660:intron 30.0%
TGATGAGAAGTTTAACACTA+GGG + chr8_3:59480204-59480223 Msa1298660:intron 30.0%
AGTTTAATTGCACTTGTACT+CGG + chr8_3:59480293-59480312 Msa1298660:intron 30.0%
AGGTACAAACCCATATTATT+AGG + chr8_3:59480434-59480453 Msa1298660:intron 30.0%
TATGCCAAATCATCTGTTAA+AGG - chr8_3:59480496-59480515 None:intergenic 30.0%
TAACATACAAAATCTGCTTG+CGG + chr8_3:59481273-59481292 Msa1298660:CDS 30.0%
! CATGATGTTTTCAATGTTGT+AGG + chr8_3:59481310-59481329 Msa1298660:CDS 30.0%
!! CTGTTTTTCTCATACAACAT+AGG - chr8_3:59481411-59481430 None:intergenic 30.0%
!! CTGTTTTTCTCATACAACAT+AGG - chr8_3:59481411-59481430 None:intergenic 30.0%
ATCTTGTGAAAGGATTCTTT+AGG + chr8_3:59481517-59481536 Msa1298660:intron 30.0%
GTTGAAATATAAAGGGGAAA+TGG + chr8_3:59481539-59481558 Msa1298660:intron 30.0%
! AAACACTTGGTTAACAGTTT+TGG - chr8_3:59481830-59481849 None:intergenic 30.0%
TGGTTTCTCCATTTATTGTT+TGG + chr8_3:59481893-59481912 Msa1298660:intron 30.0%
TTCTCCATTTATTGTTTGGT+AGG + chr8_3:59481897-59481916 Msa1298660:intron 30.0%
!! CTATTTCTACCATCTTCAAA+TGG - chr8_3:59481929-59481948 None:intergenic 30.0%
AATAGCTAATGAAGAGAATC+TGG + chr8_3:59481944-59481963 Msa1298660:CDS 30.0%
AATGAAAGAAAGCTGTTGTT+AGG + chr8_3:59482045-59482064 Msa1298660:exon 30.0%
TAAAAATGATAAGGGGAAAG+AGG - chr8_3:59482083-59482102 None:intergenic 30.0%
!!! TTTTATTTTCTCAGAGAAGG+GGG + chr8_3:59482098-59482117 Msa1298660:three_prime_UTR 30.0%
TAATTATGTGTTGGACAATG+AGG + chr8_3:59482121-59482140 Msa1298660:three_prime_UTR 30.0%
TAATTCAGATATTTAGCGTC+AGG + chr8_3:59482201-59482220 Msa1298660:three_prime_UTR 30.0%
AAGGAAATCAATGCACTTTA+AGG + chr8_3:59482363-59482382 Msa1298660:three_prime_UTR 30.0%
AGGAAATCAATGCACTTTAA+GGG + chr8_3:59482364-59482383 Msa1298660:three_prime_UTR 30.0%
AAGAATAAACGTAAATGCAG+AGG + chr8_3:59482436-59482455 Msa1298660:three_prime_UTR 30.0%
! GATCTAAGAAAATGATTCGG+TGG - chr8_3:59479414-59479433 None:intergenic 35.0%
AATGAGATTTGGATAGGGAA+GGG - chr8_3:59479443-59479462 None:intergenic 35.0%
AAATGAGATTTGGATAGGGA+AGG - chr8_3:59479444-59479463 None:intergenic 35.0%
! ATTATGTACATTTGAGACGG+TGG - chr8_3:59479526-59479545 None:intergenic 35.0%
TTCTCAAAGATTACCTGGTA+TGG + chr8_3:59479665-59479684 Msa1298660:CDS 35.0%
GATTACCTGGTATGGTTAAT+TGG + chr8_3:59479673-59479692 Msa1298660:CDS 35.0%
TTACCTGGTATGGTTAATTG+GGG + chr8_3:59479675-59479694 Msa1298660:CDS 35.0%
AATACGGAAATAACAGTTGG+TGG - chr8_3:59479702-59479721 None:intergenic 35.0%
GCAAATACGGAAATAACAGT+TGG - chr8_3:59479705-59479724 None:intergenic 35.0%
!! ACTGTTATTTCCGTATTTGC+AGG + chr8_3:59479705-59479724 Msa1298660:CDS 35.0%
GTATTTGCAGGCATGTTATA+TGG + chr8_3:59479717-59479736 Msa1298660:CDS 35.0%
AGGTGAAAAACTTACAACAG+AGG - chr8_3:59479763-59479782 None:intergenic 35.0%
GCTTTGCTTATTGTTAGATG+TGG + chr8_3:59480178-59480197 Msa1298660:intron 35.0%
GTTTAATTGCACTTGTACTC+GGG + chr8_3:59480294-59480313 Msa1298660:intron 35.0%
TGGGCTTCACCTAATAATAT+GGG - chr8_3:59480446-59480465 None:intergenic 35.0%
!!! AATCTTTTTGACTTCCTCGT+GGG - chr8_3:59480465-59480484 None:intergenic 35.0%
!!! TAATCTTTTTGACTTCCTCG+TGG - chr8_3:59480466-59480485 None:intergenic 35.0%
GTAGAAAAGGATGATTAAGC+CGG + chr8_3:59480639-59480658 Msa1298660:intron 35.0%
AAATGTGTAGCTCATGAAAG+TGG - chr8_3:59480756-59480775 None:intergenic 35.0%
ATCAACAATCATGTCTGTGA+GGG - chr8_3:59480994-59481013 None:intergenic 35.0%
AATCAACAATCATGTCTGTG+AGG - chr8_3:59480995-59481014 None:intergenic 35.0%
TATCTTATCACAACCAACTG+TGG + chr8_3:59481062-59481081 Msa1298660:intron 35.0%
AACCAACTGTGGAATCATAT+GGG + chr8_3:59481073-59481092 Msa1298660:intron 35.0%
TCTTTGCTCTGTTGAAACAT+GGG - chr8_3:59481103-59481122 None:intergenic 35.0%
ATCTTTGCTCTGTTGAAACA+TGG - chr8_3:59481104-59481123 None:intergenic 35.0%
CAGAAGTGATGTTGAAACTT+GGG + chr8_3:59481125-59481144 Msa1298660:CDS 35.0%
! ATAGAGAAAAAAGGGCATGT+TGG - chr8_3:59481499-59481518 None:intergenic 35.0%
GCACACATAAGTTAAACACT+TGG - chr8_3:59481843-59481862 None:intergenic 35.0%
CCAACCTACCAAACAATAAA+TGG - chr8_3:59481904-59481923 None:intergenic 35.0%
CCATTTATTGTTTGGTAGGT+TGG + chr8_3:59481901-59481920 Msa1298660:intron 35.0%
TTCAGATCAGAGAGAAGTAA+AGG + chr8_3:59481974-59481993 Msa1298660:CDS 35.0%
TCAGATCAGAGAGAAGTAAA+GGG + chr8_3:59481975-59481994 Msa1298660:CDS 35.0%
! CTAATTTTCCTATGAAGAGG+AGG - chr8_3:59482178-59482197 None:intergenic 35.0%
! GCACTAATTTTCCTATGAAG+AGG - chr8_3:59482181-59482200 None:intergenic 35.0%
CGTACTAGCAAAATAACAGA+TGG - chr8_3:59482250-59482269 None:intergenic 35.0%
!! TGCTAGTACGTTTTAAGTCT+TGG + chr8_3:59482260-59482279 Msa1298660:three_prime_UTR 35.0%
!!! CATTGATTTCCTTCTCGTTT+TGG - chr8_3:59482356-59482375 None:intergenic 35.0%
! TGAAAGAAGCTTTGTGTAAC+AGG + chr8_3:59482391-59482410 Msa1298660:three_prime_UTR 35.0%
!! AGAAAATGATTCGGTGGGTA+TGG - chr8_3:59479408-59479427 None:intergenic 40.0%
ATGAGATTTGGATAGGGAAG+GGG - chr8_3:59479442-59479461 None:intergenic 40.0%
CACCGAATGGAAATGAGATT+TGG - chr8_3:59479454-59479473 None:intergenic 40.0%
CAAATCTCATTTCCATTCGG+TGG + chr8_3:59479452-59479471 Msa1298660:five_prime_UTR 40.0%
TCAGTAGCTTCCATTGTAAG+AGG - chr8_3:59479489-59479508 None:intergenic 40.0%
CAGGATTCTCAAAGATTACC+TGG + chr8_3:59479660-59479679 Msa1298660:intron 40.0%
TTTGCAGGCATGTTATATGG+TGG + chr8_3:59479720-59479739 Msa1298660:CDS 40.0%
CATGTTATATGGTGGTAGCA+GGG + chr8_3:59479728-59479747 Msa1298660:CDS 40.0%
GAAATCTCATAGGACCTGTT+TGG + chr8_3:59479843-59479862 Msa1298660:intron 40.0%
GGAGAACATATAAGCTTTCC+AGG - chr8_3:59479968-59479987 None:intergenic 40.0%
CACTATGAGCATGTCTGAAT+TGG - chr8_3:59480037-59480056 None:intergenic 40.0%
GTGGGCTTCACCTAATAATA+TGG - chr8_3:59480447-59480466 None:intergenic 40.0%
! AAGTGTTGCTCCAAACAAGA+GGG - chr8_3:59480618-59480637 None:intergenic 40.0%
!! AAAGTGTTGCTCCAAACAAG+AGG - chr8_3:59480619-59480638 None:intergenic 40.0%
GGAGCAACACTTTGTAGAAA+AGG + chr8_3:59480626-59480645 Msa1298660:intron 40.0%
CACTTATACGGAACAGAAGA+AGG - chr8_3:59480685-59480704 None:intergenic 40.0%
TCCTCTTTGTAATTGTGCAG+TGG + chr8_3:59480828-59480847 Msa1298660:intron 40.0%
ACCACTGCACAATTACAAAG+AGG - chr8_3:59480832-59480851 None:intergenic 40.0%
ATGTATGTGAAGGCTGAGAT+AGG - chr8_3:59480948-59480967 None:intergenic 40.0%
CAACCAACTGTGGAATCATA+TGG + chr8_3:59481072-59481091 Msa1298660:intron 40.0%
ATCCCATATGATTCCACAGT+TGG - chr8_3:59481078-59481097 None:intergenic 40.0%
GCAGAAGTGATGTTGAAACT+TGG + chr8_3:59481124-59481143 Msa1298660:CDS 40.0%
TCGATTGATGAGAGATGCAA+TGG + chr8_3:59481174-59481193 Msa1298660:CDS 40.0%
TGAGAGATGCAATGGAGAAA+CGG + chr8_3:59481182-59481201 Msa1298660:CDS 40.0%
! GTTTTCAATGTTGTAGGAGC+TGG + chr8_3:59481316-59481335 Msa1298660:CDS 40.0%
TAAACCAAAAGCTGAAGCAG+TGG - chr8_3:59481347-59481366 None:intergenic 40.0%
TTTCCCTTTATGTTAGTGCC+AGG + chr8_3:59481596-59481615 Msa1298660:intron 40.0%
GATCCTGGCACTAACATAAA+GGG - chr8_3:59481602-59481621 None:intergenic 40.0%
!!! AGCAGCATTTTGTTTTCCTC+TGG + chr8_3:59481667-59481686 Msa1298660:CDS 40.0%
!!! GCAGCATTTTGTTTTCCTCT+GGG + chr8_3:59481668-59481687 Msa1298660:CDS 40.0%
ACTCGAGAAATAATACCCAG+AGG - chr8_3:59481686-59481705 None:intergenic 40.0%
GAAAAATCCTGTTGAGAGAG+AGG - chr8_3:59481777-59481796 None:intergenic 40.0%
AGGTTGGATCCATTTGAAGA+TGG + chr8_3:59481917-59481936 Msa1298660:intron 40.0%
AGAAGGGGGTAATTATGTGT+TGG + chr8_3:59482112-59482131 Msa1298660:three_prime_UTR 40.0%
ATGAACATCCTCCTCTTCAT+AGG + chr8_3:59482167-59482186 Msa1298660:three_prime_UTR 40.0%
CGTGGAATCTTTCTCAGTCA+CGG - chr8_3:59479375-59479394 None:intergenic 45.0%
CGTGACTGAGAAAGATTCCA+CGG + chr8_3:59479373-59479392 Msa1298660:five_prime_UTR 45.0%
!! AAATGATTCGGTGGGTATGG+TGG - chr8_3:59479405-59479424 None:intergenic 45.0%
! TTTTACCGCGCCTCTTACAA+TGG + chr8_3:59479476-59479495 Msa1298660:exon 45.0%
GGAAGCTACTGACAACTCAT+CGG + chr8_3:59479497-59479516 Msa1298660:CDS 45.0%
GCATGTTATATGGTGGTAGC+AGG + chr8_3:59479727-59479746 Msa1298660:CDS 45.0%
ATTATTAGGTGAAGCCCACG+AGG + chr8_3:59480448-59480467 Msa1298660:intron 45.0%
CTTCCATTTCCCCTCTTGTT+TGG + chr8_3:59480605-59480624 Msa1298660:intron 45.0%
! AGTGTTGCTCCAAACAAGAG+GGG - chr8_3:59480617-59480636 None:intergenic 45.0%
CGATCCTGGCACTAACATAA+AGG - chr8_3:59481603-59481622 None:intergenic 45.0%
TATGGCACTAGGATCAGTAC+TGG + chr8_3:59481643-59481662 Msa1298660:CDS 45.0%
ATGGCACTAGGATCAGTACT+GGG + chr8_3:59481644-59481663 Msa1298660:CDS 45.0%
GATCAGAGAGAAGTAAAGGG+TGG + chr8_3:59481978-59481997 Msa1298660:CDS 45.0%
AGCTTCCATTGTAAGAGGCG+CGG - chr8_3:59479484-59479503 None:intergenic 50.0%
GGTCCCCAATTAACCATACC+AGG - chr8_3:59479681-59479700 None:intergenic 50.0%
GTTATATGGTGGTAGCAGGG+AGG + chr8_3:59479731-59479750 Msa1298660:CDS 50.0%
GCTCCAAACAAGAGGGGAAA+TGG - chr8_3:59480611-59480630 None:intergenic 50.0%
! CGGTTCATTCGAGTTACACG+AGG + chr8_3:59481202-59481221 Msa1298660:CDS 50.0%
! TCAGCCACTGCTTCAGCTTT+TGG + chr8_3:59481340-59481359 Msa1298660:CDS 50.0%
CCATATTCCTTGCTCGCCAA+CGG - chr8_3:59481628-59481647 None:intergenic 50.0%
CGAGCAAGGAATATGGCACT+AGG + chr8_3:59481632-59481651 Msa1298660:CDS 50.0%
GTGTGATCCTCTCTCTCAAC+AGG + chr8_3:59481767-59481786 Msa1298660:intron 50.0%
ACAGGTGTGCTTGAAAGAGC+TGG + chr8_3:59482409-59482428 Msa1298660:three_prime_UTR 50.0%
CACACCTATGCTTAGCAGCA+CGG + chr8_3:59482521-59482540 Msa1298660:three_prime_UTR 50.0%
ACACCTATGCTTAGCAGCAC+GGG + chr8_3:59482522-59482541 Msa1298660:three_prime_UTR 50.0%
!! GATTCGGTGGGTATGGTGGT+GGG - chr8_3:59479401-59479420 None:intergenic 55.0%
!! TGATTCGGTGGGTATGGTGG+TGG - chr8_3:59479402-59479421 None:intergenic 55.0%
GCGCGGTAAAATCCACCGAA+TGG - chr8_3:59479467-59479486 None:intergenic 55.0%
GTTACACGAGGCGCACTAGT+TGG + chr8_3:59481214-59481233 Msa1298660:CDS 55.0%
! CTAGTTGGAGGGGTACGACT+TGG + chr8_3:59481229-59481248 Msa1298660:CDS 55.0%
AATCTGCTTGCGGAGAAGCG+AGG + chr8_3:59481283-59481302 Msa1298660:CDS 55.0%
CCGTTGGCGAGCAAGGAATA+TGG + chr8_3:59481625-59481644 Msa1298660:CDS 55.0%
GGCTCACAGCCAAAACGAGA+AGG + chr8_3:59482344-59482363 Msa1298660:three_prime_UTR 55.0%
ACACGAGGCGCACTAGTTGG+AGG + chr8_3:59481217-59481236 Msa1298660:CDS 60.0%
CACGAGGCGCACTAGTTGGA+GGG + chr8_3:59481218-59481237 Msa1298660:CDS 60.0%
ACGAGGCGCACTAGTTGGAG+GGG + chr8_3:59481219-59481238 Msa1298660:CDS 60.0%
TAGTGCCAGGATCGCTCCGT+TGG + chr8_3:59481609-59481628 Msa1298660:intron 60.0%
! CAGCCCGTGCTGCTAAGCAT+AGG - chr8_3:59482528-59482547 None:intergenic 60.0%
GATCGCTCCGTTGGCGAGCA+AGG + chr8_3:59481618-59481637 Msa1298660:CDS 65.0%
GCTCGCCAACGGAGCGATCC+TGG - chr8_3:59481617-59481636 None:intergenic 70.0%
!! GGGTATGGTGGTGGGCGCCG+TGG - chr8_3:59479393-59479412 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 59479303 59482571 59479303 ID=Msa1298660;Name=Msa1298660
chr8_3 mRNA 59479303 59482571 59479303 ID=Msa1298660-mRNA-1;Parent=Msa1298660;Name=Msa1298660-mRNA-1;_AED=0.20;_eAED=0.20;_QI=192|1|1|1|1|1|5|522|203
chr8_3 exon 59479303 59479536 59479303 ID=Msa1298660-mRNA-1:exon:17215;Parent=Msa1298660-mRNA-1
chr8_3 exon 59479663 59479767 59479663 ID=Msa1298660-mRNA-1:exon:17216;Parent=Msa1298660-mRNA-1
chr8_3 exon 59481115 59481370 59481115 ID=Msa1298660-mRNA-1:exon:17217;Parent=Msa1298660-mRNA-1
chr8_3 exon 59481612 59481689 59481612 ID=Msa1298660-mRNA-1:exon:17218;Parent=Msa1298660-mRNA-1
chr8_3 exon 59481919 59482571 59481919 ID=Msa1298660-mRNA-1:exon:17219;Parent=Msa1298660-mRNA-1
chr8_3 five_prime_UTR 59479303 59479494 59479303 ID=Msa1298660-mRNA-1:five_prime_utr;Parent=Msa1298660-mRNA-1
chr8_3 CDS 59479495 59479536 59479495 ID=Msa1298660-mRNA-1:cds;Parent=Msa1298660-mRNA-1
chr8_3 CDS 59479663 59479767 59479663 ID=Msa1298660-mRNA-1:cds;Parent=Msa1298660-mRNA-1
chr8_3 CDS 59481115 59481370 59481115 ID=Msa1298660-mRNA-1:cds;Parent=Msa1298660-mRNA-1
chr8_3 CDS 59481612 59481689 59481612 ID=Msa1298660-mRNA-1:cds;Parent=Msa1298660-mRNA-1
chr8_3 CDS 59481919 59482049 59481919 ID=Msa1298660-mRNA-1:cds;Parent=Msa1298660-mRNA-1
chr8_3 three_prime_UTR 59482050 59482571 59482050 ID=Msa1298660-mRNA-1:three_prime_utr;Parent=Msa1298660-mRNA-1
Gene Sequence

>Msa1298660

ATGGAAGCTACTGACAACTCATCGGAATCCACCGTCTCAAATGATTCTCAAAGATTACCTGGTATGGTTAATTGGGGACCACCAACTGTTATTTCCGTATTTGCAGGCATGTTATATGGTGGTAGCAGGGAGGCTTCTTCCTCTGTTAGCAAAGATGCAGAAGTGATGTTGAAACTTGGGAGCACAGAAGACAAACGAGCACAATATCGATTGATGAGAGATGCAATGGAGAAACGGTTCATTCGAGTTACACGAGGCGCACTAGTTGGAGGGGTACGACTTGGAATGTTCACTGCTGCATTTTATAACATACAAAATCTGCTTGCGGAGAAGCGAGGTGTGCATGATGTTTTCAATGTTGTAGGAGCTGGATCAGCCACTGCTTCAGCTTTTGGTTTAATAATGCCAGGATCGCTCCGTTGGCGAGCAAGGAATATGGCACTAGGATCAGTACTGGGAGCAGCATTTTGTTTTCCTCTGGGTTGGATCCATTTGAAGATGGTAGAAATAGCTAATGAAGAGAATCTGGCTGCACATTCAGATCAGAGAGAAGTAAAGGGTGGTGTTAGTGCTGCAATTGAGAGACTTGAAGAAAATTTAAACAAAAAATGA
Protein sequence

>Msa1298660

MEATDNSSESTVSNDSQRLPGMVNWGPPTVISVFAGMLYGGSREASSSVSKDAEVMLKLGSTEDKRAQYRLMRDAMEKRFIRVTRGALVGGVRLGMFTAAFYNIQNLLAEKRGVHDVFNVVGAGSATASAFGLIMPGSLRWRARNMALGSVLGAAFCFPLGWIHLKMVEIANEENLAAHSDQREVKGGVSAAIERLEENLNKK*