AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1299020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1299020 MtrunA17_Chr4g0059611 98.671 602 8 0 1 602 1 602 0.0 1223
Msa1299020 MtrunA17_Chr5g0415131 45.833 624 299 16 2 597 17 629 6.03e-161 476
Msa1299020 MtrunA17_Chr6g0488831 36.482 307 188 2 1 302 7 311 8.01e-52 191
Msa1299020 MtrunA17_Chr3g0098141 32.313 294 175 4 7 278 28 319 8.40e-38 150
Msa1299020 MtrunA17_Chr3g0098141 28.025 157 96 3 396 545 494 640 5.63e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1299020 AT5G62580.2 47.883 614 263 16 5 594 4 584 5.57e-166 487
Msa1299020 AT5G62580.1 47.883 614 263 16 5 594 4 584 5.57e-166 487
Msa1299020 AT1G27210.1 45.893 560 252 13 6 543 34 564 5.64e-142 426
Msa1299020 AT1G59850.1 49.455 275 137 1 6 278 21 295 4.83e-77 253
Msa1299020 AT2G07170.1 28.661 635 345 16 7 580 27 614 1.85e-65 229
Msa1299020 AT4G27060.1 30.347 547 326 11 7 517 42 569 1.37e-59 214
Msa1299020 AT1G50890.1 32.517 286 177 4 7 278 37 320 8.80e-43 165

Find 208 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGATTTCTACCCTCTTTC+AGG 0.139158 8_3:+60031224 Msa1299020:three_prime_UTR
AATAGTGCTCGATTCATTTC+TGG 0.163240 8_3:-60029726 None:intergenic
TCTTAAGCGTGAAGTATTTA+AGG 0.177171 8_3:+60028670 Msa1299020:CDS
TCACACTTCGTTGAAGAATT+TGG 0.206334 8_3:+60028751 Msa1299020:CDS
CTTGAACATCCCAATTCTTT+TGG 0.251195 8_3:-60029611 None:intergenic
TCTATATGTGCCTGTCATTA+TGG 0.271822 8_3:+60031183 Msa1299020:three_prime_UTR
GAAAATTAAACCAAAAGAAT+TGG 0.280560 8_3:+60029601 Msa1299020:CDS
CCTTCTCTTATTACTACTTA+AGG 0.285771 8_3:-60029562 None:intergenic
TTAGAAGAAGATTGTGATTT+CGG 0.288500 8_3:-60029108 None:intergenic
GGCGGAGACGCTGGTGGTTT+TGG 0.295029 8_3:+60028829 Msa1299020:CDS
CAGCATTCCTGCCAGCTGTT+TGG 0.303785 8_3:-60030383 None:intergenic
CAAGAACAGAACGCGCAAAT+TGG 0.304393 8_3:+60028557 Msa1299020:CDS
TAGTAATAAGAGAAGGAGTT+TGG 0.307829 8_3:+60029569 Msa1299020:CDS
CTTCCGTACCGGAGATTTAT+CGG 0.320464 8_3:-60028315 None:intergenic
CGTGAACCTTCTTTATAGAT+TGG 0.329359 8_3:-60030507 None:intergenic
CTCCAGGAGAGGGATGAAAA+TGG 0.330109 8_3:+60029657 Msa1299020:CDS
CTTCACGCTTAAGAAGCTTC+TGG 0.338758 8_3:-60028661 None:intergenic
TCATCAGTTGATTCTCAATC+AGG 0.340194 8_3:+60030700 Msa1299020:three_prime_UTR
GGATGATATCTCTTATGATT+TGG 0.342193 8_3:+60030194 Msa1299020:CDS
TTCATCGGAAGCTCACAAAA+TGG 0.351864 8_3:+60030126 Msa1299020:CDS
TACTTGCGCACGATGATCAT+TGG 0.356977 8_3:+60030911 Msa1299020:three_prime_UTR
CGATTTGTTGCAACTGGTTG+CGG 0.364471 8_3:-60029896 None:intergenic
ACTCTACTCAAGAGCTGGTT+GGG 0.365406 8_3:-60027862 None:intergenic
CTCCATCGAGCCTGAATCTC+TGG 0.366278 8_3:-60030422 None:intergenic
GCTTCTCGATTTGTTGCAAC+TGG 0.369994 8_3:-60029902 None:intergenic
CGTGGAGAGGGATTTCTTAT+CGG 0.371864 8_3:+60028856 Msa1299020:CDS
GTTCAGTAATGGCGAATCTA+AGG 0.377007 8_3:+60029466 Msa1299020:CDS
TCAAGTGGCCATGGCTGATC+AGG 0.377207 8_3:+60029629 Msa1299020:CDS
GAAGAATTTGGTTCCGTGTT+TGG 0.392603 8_3:+60028763 Msa1299020:CDS
AGTGTCGCGGTCGGAGAGTT+TGG 0.392872 8_3:-60028195 None:intergenic
AATCGTTGAGCAATCGTGAT+TGG 0.398731 8_3:+60028789 Msa1299020:CDS
GGATGAAAATGGAACTCCTT+TGG 0.400705 8_3:+60029668 Msa1299020:CDS
TTTGCTAATCTAGCAGGTTC+CGG 0.403125 8_3:-60028623 None:intergenic
ATGATCATCGTGCGCAAGTA+AGG 0.404633 8_3:-60030908 None:intergenic
AGTAATAAGAGAAGGAGTTT+GGG 0.408639 8_3:+60029570 Msa1299020:CDS
TCATTTCCAACCAAAACAAA+AGG 0.412202 8_3:-60031109 None:intergenic
TATGATTTGGCTGTATCAAA+TGG 0.412584 8_3:+60030207 Msa1299020:CDS
GGTGCCGGAGTCGGTGGTTA+CGG 0.420260 8_3:-60028114 None:intergenic
GGTGGACTGAATCTATTAAC+AGG 0.425031 8_3:-60029501 None:intergenic
GGATTTCTTATCGGAATTCA+AGG 0.428297 8_3:+60028865 Msa1299020:CDS
AAGCGCGTTACCGTGAGTTT+CGG 0.429364 8_3:-60028366 None:intergenic
TTGCTAGCAGTAGAACTATC+TGG 0.434089 8_3:-60029522 None:intergenic
AGTCTTTATGATTGCGGAGG+TGG 0.436488 8_3:-60028085 None:intergenic
GATCATTGGACAAGAATGAT+TGG 0.438884 8_3:+60030925 Msa1299020:three_prime_UTR
TTTCTCACCGGAGATGTTGC+CGG 0.441514 8_3:+60028048 Msa1299020:five_prime_UTR
GATCGACGGAGCTCCGGATC+CGG 0.442540 8_3:+60028604 Msa1299020:CDS
AAAATTAAACCAAAAGAATT+GGG 0.443670 8_3:+60029602 Msa1299020:CDS
TGCTGCACAATTTAGAAAGC+AGG 0.447396 8_3:+60030675 Msa1299020:three_prime_UTR
TATGATTGCGGAGGTGGATC+GGG 0.448528 8_3:-60028079 None:intergenic
GTTGAAGCTGGTGGCGCGTC+CGG 0.449963 8_3:+60028728 Msa1299020:CDS
TGAAGTATTTAAGGCGAAAC+CGG 0.450213 8_3:+60028679 Msa1299020:CDS
AGAACTGCGGGGATAAATAC+TGG 0.453512 8_3:-60030323 None:intergenic
AAAAGGGCGGTGAGAGGGGA+AGG 0.455385 8_3:-60031092 None:intergenic
ATCGTTGAGCAATCGTGATT+GGG 0.455566 8_3:+60028790 Msa1299020:CDS
AATCTAGCAGGTTCCGGATC+CGG 0.457015 8_3:-60028617 None:intergenic
GGCTGATCAGGGTGAACTCC+AGG 0.457494 8_3:+60029641 Msa1299020:CDS
TACTCTACTCAAGAGCTGGT+TGG 0.459268 8_3:-60027863 None:intergenic
GTGAACAGAGCTTCTGATAA+CGG 0.461029 8_3:-60028530 None:intergenic
TCGATCAATCAACATTTCTC+TGG 0.463128 8_3:-60030739 None:intergenic
GGTGGATCGGGAGGAATCGC+CGG 0.463754 8_3:-60028067 None:intergenic
TGCTGAAGAAGGCGAGAAGC+TGG 0.465424 8_3:+60030864 Msa1299020:three_prime_UTR
CACCACGTCGAGAGAACTGC+GGG 0.467055 8_3:-60030335 None:intergenic
GAATGATTGGAGTGCTTTCA+CGG 0.468973 8_3:+60030938 Msa1299020:three_prime_UTR
AATTCATGTTGCTTGCTACC+TGG 0.471041 8_3:+60030258 Msa1299020:CDS
TCCGTATCATGAAGAGAGTC+AGG 0.477533 8_3:+60029800 Msa1299020:CDS
TACATGCAAGAGAGGAAAAC+CGG 0.477573 8_3:-60028278 None:intergenic
TTAAGAAGCTTCTGGAACTT+CGG 0.478436 8_3:-60028653 None:intergenic
AACAGCTTTGCTAATCTAGC+AGG 0.480437 8_3:-60028629 None:intergenic
TTCCGCGATCGACGGAGCTC+CGG 0.480465 8_3:+60028598 Msa1299020:CDS
GAAGATTGTGATTTCGGAGG+TGG 0.480954 8_3:-60029102 None:intergenic
TTATGATTGCGGAGGTGGAT+CGG 0.487731 8_3:-60028080 None:intergenic
CAAGACTATGCCTGGTCCAT+AGG 0.490752 8_3:-60030535 None:intergenic
GAGTCTACTTGATCTTCTGC+AGG 0.492839 8_3:+60029932 Msa1299020:CDS
TTCACTTTGCTTGTTTCCAA+AGG 0.493034 8_3:-60029684 None:intergenic
GATAGCAACTTAGATAAATA+CGG 0.496058 8_3:-60031069 None:intergenic
CAAAACAAAAGGGCGGTGAG+AGG 0.496670 8_3:-60031098 None:intergenic
GGAATCGCCGGCAACATCTC+CGG 0.497413 8_3:-60028055 None:intergenic
ATATGACACTATTGTAAAAG+AGG 0.498872 8_3:+60030965 Msa1299020:three_prime_UTR
TCTCAAAATTCATGTAATCC+AGG 0.499696 8_3:+60029444 Msa1299020:CDS
ATTCTTCAACGAAGTGTGAC+CGG 0.501369 8_3:-60028747 None:intergenic
AAATCACAATCTTCTTCTAA+AGG 0.501812 8_3:+60029111 Msa1299020:CDS
GAAAGCTGCGGCGGAGACGC+TGG 0.503998 8_3:+60028820 Msa1299020:CDS
TGAAGTTATGAATCAGATGT+TGG 0.505651 8_3:+60029038 Msa1299020:CDS
AGATTCGCCATTACTGAACC+TGG 0.505669 8_3:-60029462 None:intergenic
GATGAGTTCTGTGGCGGCAA+GGG 0.505954 8_3:-60028222 None:intergenic
ACTATATTATATAGAGTGCC+AGG 0.506674 8_3:+60031007 Msa1299020:three_prime_UTR
AAAACAAAAGGGCGGTGAGA+GGG 0.508041 8_3:-60031097 None:intergenic
GGAGTCGGTGGTTACGGTGG+AGG 0.508645 8_3:-60028108 None:intergenic
CAGAGGTACACACTAATTCA+AGG 0.508694 8_3:+60030466 Msa1299020:CDS
GTTATCAGAAGCTCTGTTCA+CGG 0.509351 8_3:+60028532 Msa1299020:CDS
ACATGGCCTTGAGTTGGCAT+TGG 0.514122 8_3:+60030173 Msa1299020:CDS
CCTGCCAGCTGTTTGGTGCA+TGG 0.518138 8_3:-60030376 None:intergenic
AATCAACATTTCTCTGGTCA+AGG 0.520569 8_3:-60030733 None:intergenic
CCGGCGTTATTGACGCTGAT+CGG 0.523665 8_3:+60028698 Msa1299020:CDS
TCTTTAGAGACCCGTGTACA+TGG 0.524010 8_3:+60030156 Msa1299020:CDS
GAGTTGAATGATGCATCAAT+TGG 0.524050 8_3:-60030599 None:intergenic
ACCGCAGAACGTAAAACAAA+AGG 0.524333 8_3:+60028157 Msa1299020:CDS
GCGAGGATCTTCGAGAGGTA+AGG 0.524463 8_3:-60028392 None:intergenic
ATCGGAAGTGTTGTTGAAGC+TGG 0.525821 8_3:+60028716 Msa1299020:CDS
GATAGTGCCAAGACTATGCC+TGG 0.527497 8_3:-60030543 None:intergenic
CAACATCTCCGGTGAGAAAG+TGG 0.527557 8_3:-60028044 None:intergenic
GGCTCAGAGAATGCAAGTGA+TGG 0.530432 8_3:+60029405 Msa1299020:intron
GTGGCAAGATAATGCAGAAA+CGG 0.533307 8_3:-60028474 None:intergenic
TTCTGATAACGGTTTCAGAA+AGG 0.534166 8_3:-60028519 None:intergenic
GGTTTCAGAAAGGTATAAAA+CGG 0.534530 8_3:-60028509 None:intergenic
CATTTCCAACCAAAACAAAA+GGG 0.535172 8_3:-60031108 None:intergenic
TCCTGACTCTCTTCATGATA+CGG 0.537740 8_3:-60029801 None:intergenic
TGTCTTGCTTCCGCGATCGA+CGG 0.538457 8_3:+60028590 Msa1299020:CDS
GCTACCATGCACCAAACAGC+TGG 0.539989 8_3:+60030372 Msa1299020:CDS
TCTTTGGAAACATTATTGAC+AGG 0.543816 8_3:-60029831 None:intergenic
AAAACGGTTGCTTCGTGACG+TGG 0.548441 8_3:-60028493 None:intergenic
CGGAATTCACACACGCGGAT+CGG 0.552405 8_3:-60028454 None:intergenic
TGAATCAGATGTTGGAAGCA+TGG 0.553306 8_3:+60029046 Msa1299020:CDS
TAGTGATATACCATAATGAC+AGG 0.555053 8_3:-60031193 None:intergenic
AGCCAGAGATTCAGGCTCGA+TGG 0.555726 8_3:+60030420 Msa1299020:CDS
CCATGCACCAAACAGCTGGC+AGG 0.559148 8_3:+60030376 Msa1299020:CDS
AAACAAAAGGGCGGTGAGAG+GGG 0.559673 8_3:-60031096 None:intergenic
TGATGAGTTCTGTGGCGGCA+AGG 0.561237 8_3:-60028223 None:intergenic
TATCACTAATCCTCTGGCAG+AGG 0.562174 8_3:+60030449 Msa1299020:CDS
GCCGGAGTCGGTGGTTACGG+TGG 0.562226 8_3:-60028111 None:intergenic
GGTGAAGGGGAGCCTGAAAG+AGG 0.563399 8_3:-60031236 None:intergenic
ATCCGGAGCTCCGTCGATCG+CGG 0.564038 8_3:-60028600 None:intergenic
TGAACGCATTGCTTCCGTAC+CGG 0.564782 8_3:-60028326 None:intergenic
CCTCGACACAACCACCGTAC+CGG 0.564949 8_3:+60028259 Msa1299020:CDS
GGTTGTGAAGGTGCCGGAGT+CGG 0.565007 8_3:-60028123 None:intergenic
AGATTTATCGGAAGCGTCGG+TGG 0.568710 8_3:-60028303 None:intergenic
TCATAGCCAATCTATAAAGA+AGG 0.569844 8_3:+60030501 Msa1299020:three_prime_UTR
GCGTGGGAGTTATGTGAGAA+CGG 0.571788 8_3:-60028012 None:intergenic
CTATGGACCAGGCATAGTCT+TGG 0.572278 8_3:+60030536 Msa1299020:three_prime_UTR
AGTCTTGGCACTATCACCCA+TGG 0.574191 8_3:+60030551 Msa1299020:three_prime_UTR
ATCACAAAGATGCATTCCCA+TGG 0.576419 8_3:-60030568 None:intergenic
TTAGATAAATACGGAGTACA+TGG 0.578157 8_3:-60031060 None:intergenic
ATCAACATTTCTCTGGTCAA+GGG 0.579894 8_3:-60030732 None:intergenic
CCAACTCAAGGCCATGTACA+CGG 0.583283 8_3:-60030167 None:intergenic
CTCAACGATTCCACCAAACA+CGG 0.583409 8_3:-60028776 None:intergenic
CGTGAAGACGAAGATGCAAA+CGG 0.584101 8_3:-60027968 None:intergenic
ACATTCTTACAGAAATTCAT+CGG 0.584524 8_3:+60030111 Msa1299020:intron
TATGACACTATTGTAAAAGA+GGG 0.587661 8_3:+60030966 Msa1299020:three_prime_UTR
TGGAGGAGTCTTTATGATTG+CGG 0.589386 8_3:-60028091 None:intergenic
CAACTCAAGGCCATGTACAC+GGG 0.590691 8_3:-60030166 None:intergenic
GCTCAGAGAATGCAAGTGAT+GGG 0.590818 8_3:+60029406 Msa1299020:intron
CGACGCTTCCGATAAATCTC+CGG 0.590939 8_3:+60028307 Msa1299020:CDS
GGCGGTGAGGAAAGCTGCGG+CGG 0.592263 8_3:+60028811 Msa1299020:CDS
ATGTAATCCAGGTTCAGTAA+TGG 0.594185 8_3:+60029455 Msa1299020:CDS
TGTCTTGTTAATGCTGAAGA+AGG 0.596709 8_3:+60030853 Msa1299020:three_prime_UTR
AAAACAAACCACTTTCTCAC+CGG 0.598721 8_3:+60028036 Msa1299020:five_prime_UTR
TCCACCGTAACCACCGACTC+CGG 0.599380 8_3:+60028110 Msa1299020:five_prime_UTR
GAATTTGGTTCCGTGTTTGG+TGG 0.601791 8_3:+60028766 Msa1299020:CDS
GAAGAAGATTGTGATTTCGG+AGG 0.604897 8_3:-60029105 None:intergenic
AAAGAATTGGGATGTTCAAG+TGG 0.606059 8_3:+60029614 Msa1299020:CDS
CGGTGGAGTACATGCAAGAG+AGG 0.606172 8_3:-60028286 None:intergenic
TGGTGCATGGTAGCAAGTGA+TGG 0.606370 8_3:-60030363 None:intergenic
CGGAGATTTATCGGAAGCGT+CGG 0.606842 8_3:-60028306 None:intergenic
CCTTAAGTAGTAATAAGAGA+AGG 0.608938 8_3:+60029562 Msa1299020:CDS
AGCTGCGGCGGAGACGCTGG+TGG 0.609203 8_3:+60028823 Msa1299020:CDS
GAGTTCACCCTGATCAGCCA+TGG 0.611946 8_3:-60029637 None:intergenic
TTGGGATGTTCAAGTGGCCA+TGG 0.613475 8_3:+60029620 Msa1299020:CDS
AAAGGGCGGTGAGAGGGGAA+GGG 0.614806 8_3:-60031091 None:intergenic
GTGAAGGGGAGCCTGAAAGA+GGG 0.617163 8_3:-60031235 None:intergenic
TGTGAAGGTGCCGGAGTCGG+TGG 0.618376 8_3:-60028120 None:intergenic
GTTCTGCGGTGACGACATTG+TGG 0.618381 8_3:-60028144 None:intergenic
TATCATCCAATGCCAACTCA+AGG 0.619787 8_3:-60030179 None:intergenic
GTCTTGGCACTATCACCCAT+GGG 0.619828 8_3:+60030552 Msa1299020:three_prime_UTR
CATTGTGGTTGTGAAGGTGC+CGG 0.625292 8_3:-60028129 None:intergenic
ATCAGGGTGAACTCCAGGAG+AGG 0.625370 8_3:+60029646 Msa1299020:CDS
GGATTACTCTCCGAAACTCA+CGG 0.627716 8_3:+60028356 Msa1299020:CDS
AAGGGAGTGAGTGTCGCGGT+CGG 0.628539 8_3:-60028204 None:intergenic
CCGGTACGGTGGTTGTGTCG+AGG 0.630766 8_3:-60028259 None:intergenic
CCGTGTACATGGCCTTGAGT+TGG 0.633390 8_3:+60030167 Msa1299020:CDS
GATTGCGGAGGTGGATCGGG+AGG 0.636299 8_3:-60028076 None:intergenic
CAAGTGGCCATGGCTGATCA+GGG 0.636395 8_3:+60029630 Msa1299020:CDS
CAGTAATGGCGAATCTAAGG+AGG 0.638842 8_3:+60029469 Msa1299020:CDS
GGAAGTGTTGTTGAAGCTGG+TGG 0.639352 8_3:+60028719 Msa1299020:CDS
TGACGACATTGTGGTTGTGA+AGG 0.639933 8_3:-60028135 None:intergenic
TCAGGGTGAACTCCAGGAGA+GGG 0.640702 8_3:+60029647 Msa1299020:CDS
CCGATCAGCGTCAATAACGC+CGG 0.640780 8_3:-60028698 None:intergenic
AGGAGTCTTTATGATTGCGG+AGG 0.642732 8_3:-60028088 None:intergenic
CAGAGATTCAGGCTCGATGG+AGG 0.644429 8_3:+60030423 Msa1299020:CDS
TCACAAAGATGCATTCCCAT+GGG 0.647435 8_3:-60030567 None:intergenic
ATTTGGCTGTATCAAATGGA+AGG 0.647861 8_3:+60030211 Msa1299020:CDS
TGAGAATCGACGATTTGACA+AGG 0.648989 8_3:+60028907 Msa1299020:CDS
CTGCATTTCGTACTTTGTCG+CGG 0.650646 8_3:+60030649 Msa1299020:three_prime_UTR
TAAACTCTATCAAGGTGAAG+GGG 0.655350 8_3:-60031249 None:intergenic
AAAGCATGGTGGATCTCGTG+TGG 0.659084 8_3:+60029776 Msa1299020:CDS
CTTCCGATAAATCTCCGGTA+CGG 0.659557 8_3:+60028312 Msa1299020:CDS
AAGAGGGTAGAAATCTGTCA+AGG 0.663112 8_3:-60031219 None:intergenic
GCAAGAGAGGAAAACCGGTA+CGG 0.663240 8_3:-60028273 None:intergenic
GATAGTTCTACTGCTAGCAA+TGG 0.663534 8_3:+60029525 Msa1299020:CDS
CGTTGGCGAGGATCTTCGAG+AGG 0.666195 8_3:-60028397 None:intergenic
GCAATCGTGATTGGGCGGTG+AGG 0.672486 8_3:+60028798 Msa1299020:CDS
CTAGCAGTAGAACTATCTGG+TGG 0.675785 8_3:-60029519 None:intergenic
CAGAGAATGCAAGTGATGGG+CGG 0.677967 8_3:+60029409 Msa1299020:intron
TCAGCATTAACAAGACAACA+TGG 0.681390 8_3:-60030847 None:intergenic
TTCCAACCAAAACAAAAGGG+CGG 0.683986 8_3:-60031105 None:intergenic
TTAGTGTGTACCTCTGCCAG+AGG 0.685485 8_3:-60030459 None:intergenic
GTTGAGCAATCGTGATTGGG+CGG 0.693426 8_3:+60028793 Msa1299020:CDS
AGCAAGCAACATGAATTACG+AGG 0.696945 8_3:-60030252 None:intergenic
ACGGAGGCGACGAATTACGT+TGG 0.697544 8_3:-60028414 None:intergenic
AGAGAGGAAAACCGGTACGG+TGG 0.698045 8_3:-60028270 None:intergenic
CGGCAAGGGAGTGAGTGTCG+CGG 0.698773 8_3:-60028208 None:intergenic
AAATTCTGGAGGAAAACACA+AGG 0.704372 8_3:+60030300 Msa1299020:CDS
TTGGGCGGTGAGGAAAGCTG+CGG 0.707091 8_3:+60028808 Msa1299020:CDS
AATCGTGATGAGTTCTGTGG+CGG 0.713901 8_3:-60028228 None:intergenic
CCACCACGTCGAGAGAACTG+CGG 0.713909 8_3:-60030336 None:intergenic
TGCAATCGTGATGAGTTCTG+TGG 0.715694 8_3:-60028231 None:intergenic
GCAAGCAACATGAATTACGA+GGG 0.715714 8_3:-60030251 None:intergenic
AGAAACGGAATTCACACACG+CGG 0.769480 8_3:-60028459 None:intergenic
ACCACGTCGAGAGAACTGCG+GGG 0.836426 8_3:-60030334 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGAAATTATGATAAATAG+AGG + chr8_3:60029260-60029279 Msa1299020:intron 15.0%
!! TAGAAATTATGATAAATAGA+GGG + chr8_3:60029261-60029280 Msa1299020:intron 15.0%
!! AAAATTAAACCAAAAGAATT+GGG + chr8_3:60029602-60029621 Msa1299020:CDS 15.0%
!! AATGCAGTAATAAAAAAAAA+AGG - chr8_3:60030636-60030655 None:intergenic 15.0%
!! GAAAATTAAACCAAAAGAAT+TGG + chr8_3:60029601-60029620 Msa1299020:CDS 20.0%
!! AATGTTCATCATTAAAAGAT+TGG - chr8_3:60030084-60030103 None:intergenic 20.0%
! CTTGAGTAGAGTAAAAAAAA+AGG + chr8_3:60027873-60027892 Msa1299020:five_prime_UTR 25.0%
! AAAAATCATCAGAAACATCA+GGG - chr8_3:60029080-60029099 None:intergenic 25.0%
! TTAGAAGAAGATTGTGATTT+CGG - chr8_3:60029111-60029130 None:intergenic 25.0%
! AAATCACAATCTTCTTCTAA+AGG + chr8_3:60029111-60029130 Msa1299020:CDS 25.0%
!! CTAAAGGTAGCATTAAATTT+TGG + chr8_3:60029127-60029146 Msa1299020:intron 25.0%
! GATGATAGAATAAAAAAGCA+TGG + chr8_3:60029762-60029781 Msa1299020:CDS 25.0%
! GTCATTCTTAAAAAGATCTT+TGG - chr8_3:60029850-60029869 None:intergenic 25.0%
! GGAGATAAAAAAAAGTAACT+TGG - chr8_3:60029977-60029996 None:intergenic 25.0%
!!! TGTTTTTGTCTGCAAATTTT+TGG + chr8_3:60029999-60030018 Msa1299020:intron 25.0%
! ACATTCTTACAGAAATTCAT+CGG + chr8_3:60030111-60030130 Msa1299020:intron 25.0%
!!! ATTTTTTGAGCTCTAAATTC+TGG + chr8_3:60030286-60030305 Msa1299020:CDS 25.0%
! ATATGACACTATTGTAAAAG+AGG + chr8_3:60030965-60030984 Msa1299020:three_prime_UTR 25.0%
! TATGACACTATTGTAAAAGA+GGG + chr8_3:60030966-60030985 Msa1299020:three_prime_UTR 25.0%
! GATAGCAACTTAGATAAATA+CGG - chr8_3:60031072-60031091 None:intergenic 25.0%
! GGTTTCAGAAAGGTATAAAA+CGG - chr8_3:60028512-60028531 None:intergenic 30.0%
! TCTTAAGCGTGAAGTATTTA+AGG + chr8_3:60028670-60028689 Msa1299020:CDS 30.0%
! GATTGAGATTGTGTTTGTTT+TGG + chr8_3:60028996-60029015 Msa1299020:intron 30.0%
!!! GATTGTGTTTGTTTTGGTTT+AGG + chr8_3:60029002-60029021 Msa1299020:intron 30.0%
TGAAGTTATGAATCAGATGT+TGG + chr8_3:60029038-60029057 Msa1299020:CDS 30.0%
GAAAAATCATCAGAAACATC+AGG - chr8_3:60029081-60029100 None:intergenic 30.0%
!!! ACAATCTGGCTTTCTATTTT+TGG - chr8_3:60029216-60029235 None:intergenic 30.0%
GACATTAAACAAACACAATC+TGG - chr8_3:60029230-60029249 None:intergenic 30.0%
! TTGTGATCTATTGCATTGAT+TGG + chr8_3:60029341-60029360 Msa1299020:intron 30.0%
! TTTTTCTGCGTTTGTGTTTT+TGG + chr8_3:60029384-60029403 Msa1299020:intron 30.0%
!!! TTACATGAATTTTGAGACAC+AGG - chr8_3:60029441-60029460 None:intergenic 30.0%
TCTCAAAATTCATGTAATCC+AGG + chr8_3:60029444-60029463 Msa1299020:CDS 30.0%
CCTTCTCTTATTACTACTTA+AGG - chr8_3:60029565-60029584 None:intergenic 30.0%
CCTTAAGTAGTAATAAGAGA+AGG + chr8_3:60029562-60029581 Msa1299020:CDS 30.0%
TAGTAATAAGAGAAGGAGTT+TGG + chr8_3:60029569-60029588 Msa1299020:CDS 30.0%
AGTAATAAGAGAAGGAGTTT+GGG + chr8_3:60029570-60029589 Msa1299020:CDS 30.0%
! GATAGAATAAAAAAGCATGG+TGG + chr8_3:60029765-60029784 Msa1299020:CDS 30.0%
! TCTTTGGAAACATTATTGAC+AGG - chr8_3:60029834-60029853 None:intergenic 30.0%
AAATTTGCAGACAAAAACAG+TGG - chr8_3:60029998-60030017 None:intergenic 30.0%
!! TTTGTCTGCAAATTTTTGGA+AGG + chr8_3:60030003-60030022 Msa1299020:intron 30.0%
GATGAATTTCTGTAAGAATG+TGG - chr8_3:60030112-60030131 None:intergenic 30.0%
GGATGATATCTCTTATGATT+TGG + chr8_3:60030194-60030213 Msa1299020:CDS 30.0%
! TATGATTTGGCTGTATCAAA+TGG + chr8_3:60030207-60030226 Msa1299020:CDS 30.0%
TCATAGCCAATCTATAAAGA+AGG + chr8_3:60030501-60030520 Msa1299020:three_prime_UTR 30.0%
! ACTATATTATATAGAGTGCC+AGG + chr8_3:60031007-60031026 Msa1299020:three_prime_UTR 30.0%
TTAGATAAATACGGAGTACA+TGG - chr8_3:60031063-60031082 None:intergenic 30.0%
CATTTCCAACCAAAACAAAA+GGG - chr8_3:60031111-60031130 None:intergenic 30.0%
TCATTTCCAACCAAAACAAA+AGG - chr8_3:60031112-60031131 None:intergenic 30.0%
TAGTGATATACCATAATGAC+AGG - chr8_3:60031196-60031215 None:intergenic 30.0%
! TTTTTACTCTACTCAAGAGC+TGG - chr8_3:60027870-60027889 None:intergenic 35.0%
!!! ACTGCACTGTCGTTTTATTT+GGG - chr8_3:60027933-60027952 None:intergenic 35.0%
!!! AACTGCACTGTCGTTTTATT+TGG - chr8_3:60027934-60027953 None:intergenic 35.0%
!!! AAAGTGGTTTGTTTTTGCGT+GGG - chr8_3:60028031-60028050 None:intergenic 35.0%
AAAACAAACCACTTTCTCAC+CGG + chr8_3:60028036-60028055 Msa1299020:five_prime_UTR 35.0%
!!! ACCTTTTGTTTTACGTTCTG+CGG - chr8_3:60028161-60028180 None:intergenic 35.0%
!! TTCTGATAACGGTTTCAGAA+AGG - chr8_3:60028522-60028541 None:intergenic 35.0%
!! TTAAGAAGCTTCTGGAACTT+CGG - chr8_3:60028656-60028675 None:intergenic 35.0%
! TGAAGTATTTAAGGCGAAAC+CGG + chr8_3:60028679-60028698 Msa1299020:CDS 35.0%
TCACACTTCGTTGAAGAATT+TGG + chr8_3:60028751-60028770 Msa1299020:CDS 35.0%
GGATTTCTTATCGGAATTCA+AGG + chr8_3:60028865-60028884 Msa1299020:CDS 35.0%
!!! ATGAATTTTGAGACACAGGA+GGG - chr8_3:60029437-60029456 None:intergenic 35.0%
ATGTAATCCAGGTTCAGTAA+TGG + chr8_3:60029455-60029474 Msa1299020:CDS 35.0%
! CTTGAACATCCCAATTCTTT+TGG - chr8_3:60029614-60029633 None:intergenic 35.0%
AAAGAATTGGGATGTTCAAG+TGG + chr8_3:60029614-60029633 Msa1299020:CDS 35.0%
TTCACTTTGCTTGTTTCCAA+AGG - chr8_3:60029687-60029706 None:intergenic 35.0%
AATAGTGCTCGATTCATTTC+TGG - chr8_3:60029729-60029748 None:intergenic 35.0%
! AGATTGGTTCATGCATGTAA+AGG - chr8_3:60030068-60030087 None:intergenic 35.0%
GAATTTCTGTAAGAATGTGG+AGG - chr8_3:60030109-60030128 None:intergenic 35.0%
ATTTGGCTGTATCAAATGGA+AGG + chr8_3:60030211-60030230 Msa1299020:CDS 35.0%
!!! TTTTGAGCTCTAAATTCTGG+AGG + chr8_3:60030289-60030308 Msa1299020:CDS 35.0%
! AAATTCTGGAGGAAAACACA+AGG + chr8_3:60030300-60030319 Msa1299020:CDS 35.0%
! ATTTTACAAGCCAGAGATTC+AGG + chr8_3:60030412-60030431 Msa1299020:CDS 35.0%
!! AGGTTTTATCACTAATCCTC+TGG + chr8_3:60030443-60030462 Msa1299020:CDS 35.0%
CGTGAACCTTCTTTATAGAT+TGG - chr8_3:60030510-60030529 None:intergenic 35.0%
GAGTTGAATGATGCATCAAT+TGG - chr8_3:60030602-60030621 None:intergenic 35.0%
! TCATCAGTTGATTCTCAATC+AGG + chr8_3:60030700-60030719 Msa1299020:three_prime_UTR 35.0%
ATCAACATTTCTCTGGTCAA+GGG - chr8_3:60030735-60030754 None:intergenic 35.0%
AATCAACATTTCTCTGGTCA+AGG - chr8_3:60030736-60030755 None:intergenic 35.0%
TCGATCAATCAACATTTCTC+TGG - chr8_3:60030742-60030761 None:intergenic 35.0%
TCAGCATTAACAAGACAACA+TGG - chr8_3:60030850-60030869 None:intergenic 35.0%
! TGTCTTGTTAATGCTGAAGA+AGG + chr8_3:60030853-60030872 Msa1299020:three_prime_UTR 35.0%
GATCATTGGACAAGAATGAT+TGG + chr8_3:60030925-60030944 Msa1299020:three_prime_UTR 35.0%
TTTAATGCAAAAAAGCAGCC+TGG - chr8_3:60031028-60031047 None:intergenic 35.0%
!!! TGTTTTGGTTGGAAATGAAG+TGG + chr8_3:60031114-60031133 Msa1299020:three_prime_UTR 35.0%
!!! GTTTTGGTTGGAAATGAAGT+GGG + chr8_3:60031115-60031134 Msa1299020:three_prime_UTR 35.0%
TCTATATGTGCCTGTCATTA+TGG + chr8_3:60031183-60031202 Msa1299020:three_prime_UTR 35.0%
!!! GAAAGTGGTTTGTTTTTGCG+TGG - chr8_3:60028032-60028051 None:intergenic 40.0%
TGGAGGAGTCTTTATGATTG+CGG - chr8_3:60028094-60028113 None:intergenic 40.0%
ACCGCAGAACGTAAAACAAA+AGG + chr8_3:60028157-60028176 Msa1299020:CDS 40.0%
TACATGCAAGAGAGGAAAAC+CGG - chr8_3:60028281-60028300 None:intergenic 40.0%
! AAGCAATGCGTTCAGCTTTT+CGG + chr8_3:60028335-60028354 Msa1299020:CDS 40.0%
GTGGCAAGATAATGCAGAAA+CGG - chr8_3:60028477-60028496 None:intergenic 40.0%
GTGAACAGAGCTTCTGATAA+CGG - chr8_3:60028533-60028552 None:intergenic 40.0%
GTTATCAGAAGCTCTGTTCA+CGG + chr8_3:60028532-60028551 Msa1299020:CDS 40.0%
TTTGCTAATCTAGCAGGTTC+CGG - chr8_3:60028626-60028645 None:intergenic 40.0%
AACAGCTTTGCTAATCTAGC+AGG - chr8_3:60028632-60028651 None:intergenic 40.0%
ATTCTTCAACGAAGTGTGAC+CGG - chr8_3:60028750-60028769 None:intergenic 40.0%
! GAAGAATTTGGTTCCGTGTT+TGG + chr8_3:60028763-60028782 Msa1299020:CDS 40.0%
AATCGTTGAGCAATCGTGAT+TGG + chr8_3:60028789-60028808 Msa1299020:CDS 40.0%
ATCGTTGAGCAATCGTGATT+GGG + chr8_3:60028790-60028809 Msa1299020:CDS 40.0%
TGAGAATCGACGATTTGACA+AGG + chr8_3:60028907-60028926 Msa1299020:CDS 40.0%
TGAATCAGATGTTGGAAGCA+TGG + chr8_3:60029046-60029065 Msa1299020:CDS 40.0%
GAAGAAGATTGTGATTTCGG+AGG - chr8_3:60029108-60029127 None:intergenic 40.0%
TAGATCACAAACAGACGCAT+TGG - chr8_3:60029331-60029350 None:intergenic 40.0%
!!! CATGAATTTTGAGACACAGG+AGG - chr8_3:60029438-60029457 None:intergenic 40.0%
GTTCAGTAATGGCGAATCTA+AGG + chr8_3:60029466-60029485 Msa1299020:CDS 40.0%
!! GGTGGACTGAATCTATTAAC+AGG - chr8_3:60029504-60029523 None:intergenic 40.0%
TTGCTAGCAGTAGAACTATC+TGG - chr8_3:60029525-60029544 None:intergenic 40.0%
! GATAGTTCTACTGCTAGCAA+TGG + chr8_3:60029525-60029544 Msa1299020:CDS 40.0%
GGATGAAAATGGAACTCCTT+TGG + chr8_3:60029668-60029687 Msa1299020:CDS 40.0%
TCCTGACTCTCTTCATGATA+CGG - chr8_3:60029804-60029823 None:intergenic 40.0%
TTCATCGGAAGCTCACAAAA+TGG + chr8_3:60030126-60030145 Msa1299020:CDS 40.0%
TATCATCCAATGCCAACTCA+AGG - chr8_3:60030182-60030201 None:intergenic 40.0%
GCAAGCAACATGAATTACGA+GGG - chr8_3:60030254-60030273 None:intergenic 40.0%
AGCAAGCAACATGAATTACG+AGG - chr8_3:60030255-60030274 None:intergenic 40.0%
! AATTCATGTTGCTTGCTACC+TGG + chr8_3:60030258-60030277 Msa1299020:CDS 40.0%
! CAGAGGTACACACTAATTCA+AGG + chr8_3:60030466-60030485 Msa1299020:CDS 40.0%
! GAAGGTTCACGTTTTACCTA+TGG + chr8_3:60030519-60030538 Msa1299020:three_prime_UTR 40.0%
TCACAAAGATGCATTCCCAT+GGG - chr8_3:60030570-60030589 None:intergenic 40.0%
ATCACAAAGATGCATTCCCA+TGG - chr8_3:60030571-60030590 None:intergenic 40.0%
TGCTGCACAATTTAGAAAGC+AGG + chr8_3:60030675-60030694 Msa1299020:three_prime_UTR 40.0%
! GAATGATTGGAGTGCTTTCA+CGG + chr8_3:60030938-60030957 Msa1299020:three_prime_UTR 40.0%
TTCCAACCAAAACAAAAGGG+CGG - chr8_3:60031108-60031127 None:intergenic 40.0%
AAGAGGGTAGAAATCTGTCA+AGG - chr8_3:60031222-60031241 None:intergenic 40.0%
ACAGATTTCTACCCTCTTTC+AGG + chr8_3:60031224-60031243 Msa1299020:three_prime_UTR 40.0%
!! GCTGGTTGGGTTTGTTTTCT+TGG - chr8_3:60027852-60027871 None:intergenic 45.0%
ACTCTACTCAAGAGCTGGTT+GGG - chr8_3:60027865-60027884 None:intergenic 45.0%
TACTCTACTCAAGAGCTGGT+TGG - chr8_3:60027866-60027885 None:intergenic 45.0%
CGTGAAGACGAAGATGCAAA+CGG - chr8_3:60027971-60027990 None:intergenic 45.0%
TTATGATTGCGGAGGTGGAT+CGG - chr8_3:60028083-60028102 None:intergenic 45.0%
AGTCTTTATGATTGCGGAGG+TGG - chr8_3:60028088-60028107 None:intergenic 45.0%
AGGAGTCTTTATGATTGCGG+AGG - chr8_3:60028091-60028110 None:intergenic 45.0%
TGACGACATTGTGGTTGTGA+AGG - chr8_3:60028138-60028157 None:intergenic 45.0%
AATCGTGATGAGTTCTGTGG+CGG - chr8_3:60028231-60028250 None:intergenic 45.0%
TGCAATCGTGATGAGTTCTG+TGG - chr8_3:60028234-60028253 None:intergenic 45.0%
CTTCCGATAAATCTCCGGTA+CGG + chr8_3:60028312-60028331 Msa1299020:CDS 45.0%
CTTCCGTACCGGAGATTTAT+CGG - chr8_3:60028318-60028337 None:intergenic 45.0%
GGATTACTCTCCGAAACTCA+CGG + chr8_3:60028356-60028375 Msa1299020:CDS 45.0%
AGAAACGGAATTCACACACG+CGG - chr8_3:60028462-60028481 None:intergenic 45.0%
CAAGAACAGAACGCGCAAAT+TGG + chr8_3:60028557-60028576 Msa1299020:CDS 45.0%
CTTCACGCTTAAGAAGCTTC+TGG - chr8_3:60028664-60028683 None:intergenic 45.0%
!! ATCGGAAGTGTTGTTGAAGC+TGG + chr8_3:60028716-60028735 Msa1299020:CDS 45.0%
! GAATTTGGTTCCGTGTTTGG+TGG + chr8_3:60028766-60028785 Msa1299020:CDS 45.0%
CTCAACGATTCCACCAAACA+CGG - chr8_3:60028779-60028798 None:intergenic 45.0%
CGTGGAGAGGGATTTCTTAT+CGG + chr8_3:60028856-60028875 Msa1299020:CDS 45.0%
GAAGATTGTGATTTCGGAGG+TGG - chr8_3:60029105-60029124 None:intergenic 45.0%
GCTCAGAGAATGCAAGTGAT+GGG + chr8_3:60029406-60029425 Msa1299020:intron 45.0%
AGATTCGCCATTACTGAACC+TGG - chr8_3:60029465-60029484 None:intergenic 45.0%
CAGTAATGGCGAATCTAAGG+AGG + chr8_3:60029469-60029488 Msa1299020:CDS 45.0%
CTAGCAGTAGAACTATCTGG+TGG - chr8_3:60029522-60029541 None:intergenic 45.0%
! TTCCATTTTCATCCCTCTCC+TGG - chr8_3:60029662-60029681 None:intergenic 45.0%
TCCGTATCATGAAGAGAGTC+AGG + chr8_3:60029800-60029819 Msa1299020:CDS 45.0%
!! CGATTTGTTGCAACTGGTTG+CGG - chr8_3:60029899-60029918 None:intergenic 45.0%
!! GCTTCTCGATTTGTTGCAAC+TGG - chr8_3:60029905-60029924 None:intergenic 45.0%
GAGTCTACTTGATCTTCTGC+AGG + chr8_3:60029932-60029951 Msa1299020:CDS 45.0%
! TCTTTAGAGACCCGTGTACA+TGG + chr8_3:60030156-60030175 Msa1299020:CDS 45.0%
GAGCTCAAAAAATCTGCTCC+AGG - chr8_3:60030279-60030298 None:intergenic 45.0%
AGAACTGCGGGGATAAATAC+TGG - chr8_3:60030326-60030345 None:intergenic 45.0%
TATCACTAATCCTCTGGCAG+AGG + chr8_3:60030449-60030468 Msa1299020:CDS 45.0%
! TCACGTTTTACCTATGGACC+AGG + chr8_3:60030525-60030544 Msa1299020:three_prime_UTR 45.0%
CTGCATTTCGTACTTTGTCG+CGG + chr8_3:60030649-60030668 Msa1299020:three_prime_UTR 45.0%
ATGATCATCGTGCGCAAGTA+AGG - chr8_3:60030911-60030930 None:intergenic 45.0%
TACTTGCGCACGATGATCAT+TGG + chr8_3:60030911-60030930 Msa1299020:three_prime_UTR 45.0%
AAAACAAAAGGGCGGTGAGA+GGG - chr8_3:60031100-60031119 None:intergenic 45.0%
GCGTGGGAGTTATGTGAGAA+CGG - chr8_3:60028015-60028034 None:intergenic 50.0%
CAACATCTCCGGTGAGAAAG+TGG - chr8_3:60028047-60028066 None:intergenic 50.0%
TTTCTCACCGGAGATGTTGC+CGG + chr8_3:60028048-60028067 Msa1299020:five_prime_UTR 50.0%
TATGATTGCGGAGGTGGATC+GGG - chr8_3:60028082-60028101 None:intergenic 50.0%
! CATTGTGGTTGTGAAGGTGC+CGG - chr8_3:60028132-60028151 None:intergenic 50.0%
GCAAGAGAGGAAAACCGGTA+CGG - chr8_3:60028276-60028295 None:intergenic 50.0%
!! AGATTTATCGGAAGCGTCGG+TGG - chr8_3:60028306-60028325 None:intergenic 50.0%
! CGGAGATTTATCGGAAGCGT+CGG - chr8_3:60028309-60028328 None:intergenic 50.0%
CGACGCTTCCGATAAATCTC+CGG + chr8_3:60028307-60028326 Msa1299020:CDS 50.0%
TGAACGCATTGCTTCCGTAC+CGG - chr8_3:60028329-60028348 None:intergenic 50.0%
AAGCGCGTTACCGTGAGTTT+CGG - chr8_3:60028369-60028388 None:intergenic 50.0%
AAAACGGTTGCTTCGTGACG+TGG - chr8_3:60028496-60028515 None:intergenic 50.0%
AATCTAGCAGGTTCCGGATC+CGG - chr8_3:60028620-60028639 None:intergenic 50.0%
!! GGAAGTGTTGTTGAAGCTGG+TGG + chr8_3:60028719-60028738 Msa1299020:CDS 50.0%
GTTGAGCAATCGTGATTGGG+CGG + chr8_3:60028793-60028812 Msa1299020:CDS 50.0%
!!! TGGTTTTGGCTAACGTGGAG+AGG + chr8_3:60028843-60028862 Msa1299020:CDS 50.0%
!!! GGTTTTGGCTAACGTGGAGA+GGG + chr8_3:60028844-60028863 Msa1299020:CDS 50.0%
GGCTCAGAGAATGCAAGTGA+TGG + chr8_3:60029405-60029424 Msa1299020:intron 50.0%
! CAGAGAATGCAAGTGATGGG+CGG + chr8_3:60029409-60029428 Msa1299020:intron 50.0%
TTGGGATGTTCAAGTGGCCA+TGG + chr8_3:60029620-60029639 Msa1299020:CDS 50.0%
CTCCAGGAGAGGGATGAAAA+TGG + chr8_3:60029657-60029676 Msa1299020:CDS 50.0%
!! AAAGCATGGTGGATCTCGTG+TGG + chr8_3:60029776-60029795 Msa1299020:CDS 50.0%
CAACTCAAGGCCATGTACAC+GGG - chr8_3:60030169-60030188 None:intergenic 50.0%
CCAACTCAAGGCCATGTACA+CGG - chr8_3:60030170-60030189 None:intergenic 50.0%
!! ACATGGCCTTGAGTTGGCAT+TGG + chr8_3:60030173-60030192 Msa1299020:CDS 50.0%
TGGTGCATGGTAGCAAGTGA+TGG - chr8_3:60030366-60030385 None:intergenic 50.0%
TTAGTGTGTACCTCTGCCAG+AGG - chr8_3:60030462-60030481 None:intergenic 50.0%
CAAGACTATGCCTGGTCCAT+AGG - chr8_3:60030538-60030557 None:intergenic 50.0%
CTATGGACCAGGCATAGTCT+TGG + chr8_3:60030536-60030555 Msa1299020:three_prime_UTR 50.0%
GATAGTGCCAAGACTATGCC+TGG - chr8_3:60030546-60030565 None:intergenic 50.0%
AGTCTTGGCACTATCACCCA+TGG + chr8_3:60030551-60030570 Msa1299020:three_prime_UTR 50.0%
GTCTTGGCACTATCACCCAT+GGG + chr8_3:60030552-60030571 Msa1299020:three_prime_UTR 50.0%
AAACAAAAGGGCGGTGAGAG+GGG - chr8_3:60031099-60031118 None:intergenic 50.0%
CAAAACAAAAGGGCGGTGAG+AGG - chr8_3:60031101-60031120 None:intergenic 50.0%
!! CTCTCACCGCCCTTTTGTTT+TGG + chr8_3:60031099-60031118 Msa1299020:three_prime_UTR 50.0%
!!! CACCGCCCTTTTGTTTTGGT+TGG + chr8_3:60031103-60031122 Msa1299020:three_prime_UTR 50.0%
GTTCTGCGGTGACGACATTG+TGG - chr8_3:60028147-60028166 None:intergenic 55.0%
GATGAGTTCTGTGGCGGCAA+GGG - chr8_3:60028225-60028244 None:intergenic 55.0%
TGATGAGTTCTGTGGCGGCA+AGG - chr8_3:60028226-60028245 None:intergenic 55.0%
AGAGAGGAAAACCGGTACGG+TGG - chr8_3:60028273-60028292 None:intergenic 55.0%
CGGTGGAGTACATGCAAGAG+AGG - chr8_3:60028289-60028308 None:intergenic 55.0%
GCGAGGATCTTCGAGAGGTA+AGG - chr8_3:60028395-60028414 None:intergenic 55.0%
ACGGAGGCGACGAATTACGT+TGG - chr8_3:60028417-60028436 None:intergenic 55.0%
CGGAATTCACACACGCGGAT+CGG - chr8_3:60028457-60028476 None:intergenic 55.0%
TGTCTTGCTTCCGCGATCGA+CGG + chr8_3:60028590-60028609 Msa1299020:CDS 55.0%
CCGATCAGCGTCAATAACGC+CGG - chr8_3:60028701-60028720 None:intergenic 55.0%
CCGGCGTTATTGACGCTGAT+CGG + chr8_3:60028698-60028717 Msa1299020:CDS 55.0%
!!! GCTGGTGGTTTTGGCTAACG+TGG + chr8_3:60028838-60028857 Msa1299020:CDS 55.0%
TCAAGTGGCCATGGCTGATC+AGG + chr8_3:60029629-60029648 Msa1299020:CDS 55.0%
CAAGTGGCCATGGCTGATCA+GGG + chr8_3:60029630-60029649 Msa1299020:CDS 55.0%
GAGTTCACCCTGATCAGCCA+TGG - chr8_3:60029640-60029659 None:intergenic 55.0%
ATCAGGGTGAACTCCAGGAG+AGG + chr8_3:60029646-60029665 Msa1299020:CDS 55.0%
TCAGGGTGAACTCCAGGAGA+GGG + chr8_3:60029647-60029666 Msa1299020:CDS 55.0%
! CCGTGTACATGGCCTTGAGT+TGG + chr8_3:60030167-60030186 Msa1299020:CDS 55.0%
GCTACCATGCACCAAACAGC+TGG + chr8_3:60030372-60030391 Msa1299020:CDS 55.0%
CAGCATTCCTGCCAGCTGTT+TGG - chr8_3:60030386-60030405 None:intergenic 55.0%
AGCCAGAGATTCAGGCTCGA+TGG + chr8_3:60030420-60030439 Msa1299020:CDS 55.0%
CTCCATCGAGCCTGAATCTC+TGG - chr8_3:60030425-60030444 None:intergenic 55.0%
CAGAGATTCAGGCTCGATGG+AGG + chr8_3:60030423-60030442 Msa1299020:CDS 55.0%
TGCTGAAGAAGGCGAGAAGC+TGG + chr8_3:60030864-60030883 Msa1299020:three_prime_UTR 55.0%
GGAATCGCCGGCAACATCTC+CGG - chr8_3:60028058-60028077 None:intergenic 60.0%
!! TCCACCGTAACCACCGACTC+CGG + chr8_3:60028110-60028129 Msa1299020:five_prime_UTR 60.0%
!! GGTTGTGAAGGTGCCGGAGT+CGG - chr8_3:60028126-60028145 None:intergenic 60.0%
AGTGTCGCGGTCGGAGAGTT+TGG - chr8_3:60028198-60028217 None:intergenic 60.0%
! AAGGGAGTGAGTGTCGCGGT+CGG - chr8_3:60028207-60028226 None:intergenic 60.0%
CCTCGACACAACCACCGTAC+CGG + chr8_3:60028259-60028278 Msa1299020:CDS 60.0%
CGTTGGCGAGGATCTTCGAG+AGG - chr8_3:60028400-60028419 None:intergenic 60.0%
GGCGACGAATTACGTTGGCG+AGG - chr8_3:60028412-60028431 None:intergenic 60.0%
!! CCTCCGTGATACCGACTCGT+CGG + chr8_3:60028430-60028449 Msa1299020:CDS 60.0%
GGACCGACGAGTCGGTATCA+CGG - chr8_3:60028436-60028455 None:intergenic 60.0%
GCAATCGTGATTGGGCGGTG+AGG + chr8_3:60028798-60028817 Msa1299020:CDS 60.0%
TTGGGCGGTGAGGAAAGCTG+CGG + chr8_3:60028808-60028827 Msa1299020:CDS 60.0%
GGCTGATCAGGGTGAACTCC+AGG + chr8_3:60029641-60029660 Msa1299020:CDS 60.0%
ACCACGTCGAGAGAACTGCG+GGG - chr8_3:60030337-60030356 None:intergenic 60.0%
CACCACGTCGAGAGAACTGC+GGG - chr8_3:60030338-60030357 None:intergenic 60.0%
CCACCACGTCGAGAGAACTG+CGG - chr8_3:60030339-60030358 None:intergenic 60.0%
!! CCTGCCAGCTGTTTGGTGCA+TGG - chr8_3:60030379-60030398 None:intergenic 60.0%
CCATGCACCAAACAGCTGGC+AGG + chr8_3:60030376-60030395 Msa1299020:CDS 60.0%
AAAGGGCGGTGAGAGGGGAA+GGG - chr8_3:60031094-60031113 None:intergenic 60.0%
AAAAGGGCGGTGAGAGGGGA+AGG - chr8_3:60031095-60031114 None:intergenic 60.0%
GGTGGATCGGGAGGAATCGC+CGG - chr8_3:60028070-60028089 None:intergenic 65.0%
GATTGCGGAGGTGGATCGGG+AGG - chr8_3:60028079-60028098 None:intergenic 65.0%
GGAGTCGGTGGTTACGGTGG+AGG - chr8_3:60028111-60028130 None:intergenic 65.0%
! GGTGCCGGAGTCGGTGGTTA+CGG - chr8_3:60028117-60028136 None:intergenic 65.0%
!! TGTGAAGGTGCCGGAGTCGG+TGG - chr8_3:60028123-60028142 None:intergenic 65.0%
! CGGCAAGGGAGTGAGTGTCG+CGG - chr8_3:60028211-60028230 None:intergenic 65.0%
CCGGTACGGTGGTTGTGTCG+AGG - chr8_3:60028262-60028281 None:intergenic 65.0%
CCGACGAGTCGGTATCACGG+AGG - chr8_3:60028433-60028452 None:intergenic 65.0%
TTCCGCGATCGACGGAGCTC+CGG + chr8_3:60028598-60028617 Msa1299020:CDS 65.0%
ATCCGGAGCTCCGTCGATCG+CGG - chr8_3:60028603-60028622 None:intergenic 65.0%
GATCGACGGAGCTCCGGATC+CGG + chr8_3:60028604-60028623 Msa1299020:CDS 65.0%
! GTTGAAGCTGGTGGCGCGTC+CGG + chr8_3:60028728-60028747 Msa1299020:CDS 65.0%
! GGCGGAGACGCTGGTGGTTT+TGG + chr8_3:60028829-60028848 Msa1299020:CDS 65.0%
TCCCCGCAGTTCTCTCGACG+TGG + chr8_3:60030333-60030352 Msa1299020:CDS 65.0%
CCGCAGTTCTCTCGACGTGG+TGG + chr8_3:60030336-60030355 Msa1299020:CDS 65.0%
GCCGGAGTCGGTGGTTACGG+TGG - chr8_3:60028114-60028133 None:intergenic 70.0%
CGCGGATCGGACCGACGAGT+CGG - chr8_3:60028444-60028463 None:intergenic 70.0%
GGCGGTGAGGAAAGCTGCGG+CGG + chr8_3:60028811-60028830 Msa1299020:CDS 70.0%
GAAAGCTGCGGCGGAGACGC+TGG + chr8_3:60028820-60028839 Msa1299020:CDS 70.0%
AGCTGCGGCGGAGACGCTGG+TGG + chr8_3:60028823-60028842 Msa1299020:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 60027847 60031256 60027847 ID=Msa1299020;Name=Msa1299020
chr8_3 mRNA 60027847 60031256 60027847 ID=Msa1299020-mRNA-1;Parent=Msa1299020;Name=Msa1299020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=302|1|1|1|1|1|4|756|602
chr8_3 exon 60027847 60028928 60027847 ID=Msa1299020-mRNA-1:exon:17428;Parent=Msa1299020-mRNA-1
chr8_3 exon 60029024 60029132 60029024 ID=Msa1299020-mRNA-1:exon:17429;Parent=Msa1299020-mRNA-1
chr8_3 exon 60029412 60029953 60029412 ID=Msa1299020-mRNA-1:exon:17430;Parent=Msa1299020-mRNA-1
chr8_3 exon 60030123 60031256 60030123 ID=Msa1299020-mRNA-1:exon:17431;Parent=Msa1299020-mRNA-1
chr8_3 five_prime_UTR 60027847 60028148 60027847 ID=Msa1299020-mRNA-1:five_prime_utr;Parent=Msa1299020-mRNA-1
chr8_3 CDS 60028149 60028928 60028149 ID=Msa1299020-mRNA-1:cds;Parent=Msa1299020-mRNA-1
chr8_3 CDS 60029024 60029132 60029024 ID=Msa1299020-mRNA-1:cds;Parent=Msa1299020-mRNA-1
chr8_3 CDS 60029412 60029953 60029412 ID=Msa1299020-mRNA-1:cds;Parent=Msa1299020-mRNA-1
chr8_3 CDS 60030123 60030500 60030123 ID=Msa1299020-mRNA-1:cds;Parent=Msa1299020-mRNA-1
chr8_3 three_prime_UTR 60030501 60031256 60030501 ID=Msa1299020-mRNA-1:three_prime_utr;Parent=Msa1299020-mRNA-1
Gene Sequence

>Msa1299020

ATGTCGTCACCGCAGAACGTAAAACAAAAGGTATTCACATGTCTAACCAAACTCTCCGACCGCGACACTCACTCCCTTGCCGCCACAGAACTCATCACGATTGCACGCAACCTCGACACAACCACCGTACCGGTTTTCCTCTCTTGCATGTACTCCACCGACGCTTCCGATAAATCTCCGGTACGGAAGCAATGCGTTCAGCTTTTCGGATTACTCTCCGAAACTCACGGTAACGCGCTTTCTCCTTACCTCTCGAAGATCCTCGCCAACGTAATTCGTCGCCTCCGTGATACCGACTCGTCGGTCCGATCCGCGTGTGTGAATTCCGTTTCTGCATTATCTTGCCACGTCACGAAGCAACCGTTTTATACCTTTCTGAAACCGTTATCAGAAGCTCTGTTCACGGAGCAAGAACAGAACGCGCAAATTGGCGCTGCACTTTGTCTTGCTTCCGCGATCGACGGAGCTCCGGATCCGGAACCTGCTAGATTAGCAAAGCTGTTGCCGAAGTTCCAGAAGCTTCTTAAGCGTGAAGTATTTAAGGCGAAACCGGCGTTATTGACGCTGATCGGAAGTGTTGTTGAAGCTGGTGGCGCGTCCGGTCACACTTCGTTGAAGAATTTGGTTCCGTGTTTGGTGGAATCGTTGAGCAATCGTGATTGGGCGGTGAGGAAAGCTGCGGCGGAGACGCTGGTGGTTTTGGCTAACGTGGAGAGGGATTTCTTATCGGAATTCAAGGCTGATTGCATGAAAGTTTTTGAGAATCGACGATTTGACAAGGTGAAGTTAGTTCGTGAAGTTATGAATCAGATGTTGGAAGCATGGAAGCATATCCCTGATGTTTCTGATGATTTTTCGCCACCTCCGAAATCACAATCTTCTTCTAAAGAGAATGCAAGTGATGGGCGGTACCCTCCTGTGTCTCAAAATTCATGTAATCCAGGTTCAGTAATGGCGAATCTAAGGAGGAGATCAGCTCCTGTTAATAGATTCAGTCCACCAGATAGTTCTACTGCTAGCAATGGCAAGAATGTGAGTTCCTTAAGTAGTAATAAGAGAAGGAGTTTGGGTGTTTCGCGAAAATTAAACCAAAAGAATTGGGATGTTCAAGTGGCCATGGCTGATCAGGGTGAACTCCAGGAGAGGGATGAAAATGGAACTCCTTTGGAAACAAGCAAAGTGAATAAAAGCAAATTTCTAAAGCCAGAAATGAATCGAGCACTATTAAATAAAAATTCTGATGATAGAATAAAAAAGCATGGTGGATCTCGTGTGGTTCCGTATCATGAAGAGAGTCAGGACTCAGTTCCTGTCAATAATGTTTCCAAAGATCTTTTTAAGAATGACAAAGAGAGTGAAGAGTTATCTTTGATCCGCAACCAGTTGCAACAAATCGAGAAGCAACAGTCGAGTCTACTTGATCTTCTGCAGAAATTCATCGGAAGCTCACAAAATGGAATGCGATCTTTAGAGACCCGTGTACATGGCCTTGAGTTGGCATTGGATGATATCTCTTATGATTTGGCTGTATCAAATGGAAGGATGACTAATTCTAATGTCCCTCGTAATTCATGTTGCTTGCTACCTGGAGCAGATTTTTTGAGCTCTAAATTCTGGAGGAAAACACAAGGCCAGTATTTATCCCCGCAGTTCTCTCGACGTGGTGGTGCGCCATCACTTGCTACCATGCACCAAACAGCTGGCAGGAATGCTGAAACTAATTTTACAAGCCAGAGATTCAGGCTCGATGGAGGTTTTATCACTAATCCTCTGGCAGAGGTACACACTAATTCAAGGAACTTTGCTTGA
Protein sequence

>Msa1299020

MSSPQNVKQKVFTCLTKLSDRDTHSLAATELITIARNLDTTTVPVFLSCMYSTDASDKSPVRKQCVQLFGLLSETHGNALSPYLSKILANVIRRLRDTDSSVRSACVNSVSALSCHVTKQPFYTFLKPLSEALFTEQEQNAQIGAALCLASAIDGAPDPEPARLAKLLPKFQKLLKREVFKAKPALLTLIGSVVEAGGASGHTSLKNLVPCLVESLSNRDWAVRKAAAETLVVLANVERDFLSEFKADCMKVFENRRFDKVKLVREVMNQMLEAWKHIPDVSDDFSPPPKSQSSSKENASDGRYPPVSQNSCNPGSVMANLRRRSAPVNRFSPPDSSTASNGKNVSSLSSNKRRSLGVSRKLNQKNWDVQVAMADQGELQERDENGTPLETSKVNKSKFLKPEMNRALLNKNSDDRIKKHGGSRVVPYHEESQDSVPVNNVSKDLFKNDKESEELSLIRNQLQQIEKQQSSLLDLLQKFIGSSQNGMRSLETRVHGLELALDDISYDLAVSNGRMTNSNVPRNSCCLLPGADFLSSKFWRKTQGQYLSPQFSRRGGAPSLATMHQTAGRNAETNFTSQRFRLDGGFITNPLAEVHTNSRNFA*