AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1259410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1259410 MtrunA17_Chr4g0059611 98.671 602 8 0 1 602 1 602 0.0 1223
Msa1259410 MtrunA17_Chr5g0415131 45.833 624 299 16 2 597 17 629 6.03e-161 476
Msa1259410 MtrunA17_Chr6g0488831 36.482 307 188 2 1 302 7 311 8.01e-52 191
Msa1259410 MtrunA17_Chr3g0098141 32.313 294 175 4 7 278 28 319 8.40e-38 150
Msa1259410 MtrunA17_Chr3g0098141 28.025 157 96 3 396 545 494 640 5.63e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1259410 AT5G62580.2 47.883 614 263 16 5 594 4 584 5.57e-166 487
Msa1259410 AT5G62580.1 47.883 614 263 16 5 594 4 584 5.57e-166 487
Msa1259410 AT1G27210.1 45.893 560 252 13 6 543 34 564 5.64e-142 426
Msa1259410 AT1G59850.1 49.455 275 137 1 6 278 21 295 4.83e-77 253
Msa1259410 AT2G07170.1 28.661 635 345 16 7 580 27 614 1.85e-65 229
Msa1259410 AT4G27060.1 30.347 547 326 11 7 517 42 569 1.37e-59 214
Msa1259410 AT1G50890.1 32.517 286 177 4 7 278 37 320 8.80e-43 165

Find 208 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGATTTCTACCCTCTTTC+AGG 0.139158 8_2:+61067707 Msa1259410:three_prime_UTR
AATAGTGCTCGATTCATTTC+TGG 0.163240 8_2:-61066208 None:intergenic
TCTTAAGCGTGAAGTATTTA+AGG 0.177171 8_2:+61065152 Msa1259410:CDS
TCACACTTCGTTGAAGAATT+TGG 0.206334 8_2:+61065233 Msa1259410:CDS
CTTGAACATCCCAATTCTTT+TGG 0.251195 8_2:-61066093 None:intergenic
TCTATATGTGCCTGTCATTA+TGG 0.271822 8_2:+61067666 Msa1259410:three_prime_UTR
GAAAATTAAACCAAAAGAAT+TGG 0.280560 8_2:+61066083 Msa1259410:CDS
CCTTCTCTTATTACTACTTA+AGG 0.285771 8_2:-61066044 None:intergenic
TTAGAAGAAGATTGTGATTT+CGG 0.288500 8_2:-61065590 None:intergenic
GGCGGAGACGCTGGTGGTTT+TGG 0.295029 8_2:+61065311 Msa1259410:CDS
CAGCATTCCTGCCAGCTGTT+TGG 0.303785 8_2:-61066865 None:intergenic
CAAGAACAGAACGCGCAAAT+TGG 0.304393 8_2:+61065039 Msa1259410:CDS
TAGTAATAAGAGAAGGAGTT+TGG 0.307829 8_2:+61066051 Msa1259410:CDS
CTTCCGTACCGGAGATTTAT+CGG 0.320464 8_2:-61064797 None:intergenic
CGTGAACCTTCTTTATAGAT+TGG 0.329359 8_2:-61066989 None:intergenic
CTCCAGGAGAGGGATGAAAA+TGG 0.330109 8_2:+61066139 Msa1259410:CDS
CTTCACGCTTAAGAAGCTTC+TGG 0.338758 8_2:-61065143 None:intergenic
TCATCAGTTGATTCTCAATC+AGG 0.340194 8_2:+61067182 Msa1259410:three_prime_UTR
GGATGATATCTCTTATGATT+TGG 0.342193 8_2:+61066676 Msa1259410:CDS
TTCATCGGAAGCTCACAAAA+TGG 0.351864 8_2:+61066608 Msa1259410:CDS
TACTTGCGCACGATGATCAT+TGG 0.356977 8_2:+61067394 Msa1259410:three_prime_UTR
CGATTTGTTGCAACTGGTTG+CGG 0.364471 8_2:-61066378 None:intergenic
ACTCTACTCAAGAGCTGGTT+GGG 0.365406 8_2:-61064342 None:intergenic
CTCCATCGAGCCTGAATCTC+TGG 0.366278 8_2:-61066904 None:intergenic
GCTTCTCGATTTGTTGCAAC+TGG 0.369994 8_2:-61066384 None:intergenic
CGTGGAGAGGGATTTCTTAT+CGG 0.371864 8_2:+61065338 Msa1259410:CDS
GTTCAGTAATGGCGAATCTA+AGG 0.377007 8_2:+61065948 Msa1259410:CDS
TCAAGTGGCCATGGCTGATC+AGG 0.377207 8_2:+61066111 Msa1259410:CDS
GAAGAATTTGGTTCCGTGTT+TGG 0.392603 8_2:+61065245 Msa1259410:CDS
AGTGTCGCGGTCGGAGAGTT+TGG 0.392872 8_2:-61064677 None:intergenic
AATCGTTGAGCAATCGTGAT+TGG 0.398731 8_2:+61065271 Msa1259410:CDS
GGATGAAAATGGAACTCCTT+TGG 0.400705 8_2:+61066150 Msa1259410:CDS
TTTGCTAATCTAGCAGGTTC+CGG 0.403125 8_2:-61065105 None:intergenic
ATGATCATCGTGCGCAAGTA+AGG 0.404633 8_2:-61067391 None:intergenic
AGTAATAAGAGAAGGAGTTT+GGG 0.408639 8_2:+61066052 Msa1259410:CDS
TCATTTCCAACCAAAACAAA+AGG 0.412202 8_2:-61067592 None:intergenic
TATGATTTGGCTGTATCAAA+TGG 0.412584 8_2:+61066689 Msa1259410:CDS
GGTGCCGGAGTCGGTGGTTA+CGG 0.420260 8_2:-61064596 None:intergenic
GGTGGACTGAATCTATTAAC+AGG 0.425031 8_2:-61065983 None:intergenic
GGATTTCTTATCGGAATTCA+AGG 0.428297 8_2:+61065347 Msa1259410:CDS
AAGCGCGTTACCGTGAGTTT+CGG 0.429364 8_2:-61064848 None:intergenic
TTGCTAGCAGTAGAACTATC+TGG 0.434089 8_2:-61066004 None:intergenic
AGTCTTTATGATTGCGGAGG+TGG 0.436488 8_2:-61064567 None:intergenic
GATCATTGGACAAGAATGAT+TGG 0.438884 8_2:+61067408 Msa1259410:three_prime_UTR
TTTCTCACCGGAGATGTTGC+CGG 0.441514 8_2:+61064530 Msa1259410:five_prime_UTR
GATCGACGGAGCTCCGGATC+CGG 0.442540 8_2:+61065086 Msa1259410:CDS
AAAATTAAACCAAAAGAATT+GGG 0.443670 8_2:+61066084 Msa1259410:CDS
TGCTGCACAATTTAGAAAGC+AGG 0.447396 8_2:+61067157 Msa1259410:three_prime_UTR
TATGATTGCGGAGGTGGATC+GGG 0.448528 8_2:-61064561 None:intergenic
GTTGAAGCTGGTGGCGCGTC+CGG 0.449963 8_2:+61065210 Msa1259410:CDS
TGAAGTATTTAAGGCGAAAC+CGG 0.450213 8_2:+61065161 Msa1259410:CDS
AGAACTGCGGGGATAAATAC+TGG 0.453512 8_2:-61066805 None:intergenic
AAAAGGGCGGTGAGAGGGGA+AGG 0.455385 8_2:-61067575 None:intergenic
ATCGTTGAGCAATCGTGATT+GGG 0.455566 8_2:+61065272 Msa1259410:CDS
AATCTAGCAGGTTCCGGATC+CGG 0.457015 8_2:-61065099 None:intergenic
GGCTGATCAGGGTGAACTCC+AGG 0.457494 8_2:+61066123 Msa1259410:CDS
TACTCTACTCAAGAGCTGGT+TGG 0.459268 8_2:-61064343 None:intergenic
GTGAACAGAGCTTCTGATAA+CGG 0.461029 8_2:-61065012 None:intergenic
TCGATCAATCAACATTTCTC+TGG 0.463128 8_2:-61067221 None:intergenic
GGTGGATCGGGAGGAATCGC+CGG 0.463754 8_2:-61064549 None:intergenic
TGCTGAAGAAGGCGAGAAGC+TGG 0.465424 8_2:+61067347 Msa1259410:three_prime_UTR
CACCACGTCGAGAGAACTGC+GGG 0.467055 8_2:-61066817 None:intergenic
GAATGATTGGAGTGCTTTCA+CGG 0.468973 8_2:+61067421 Msa1259410:three_prime_UTR
AATTCATGTTGCTTGCTACC+TGG 0.471041 8_2:+61066740 Msa1259410:CDS
TCCGTATCATGAAGAGAGTC+AGG 0.477533 8_2:+61066282 Msa1259410:CDS
TACATGCAAGAGAGGAAAAC+CGG 0.477573 8_2:-61064760 None:intergenic
TTAAGAAGCTTCTGGAACTT+CGG 0.478436 8_2:-61065135 None:intergenic
AACAGCTTTGCTAATCTAGC+AGG 0.480437 8_2:-61065111 None:intergenic
TTCCGCGATCGACGGAGCTC+CGG 0.480465 8_2:+61065080 Msa1259410:CDS
GAAGATTGTGATTTCGGAGG+TGG 0.480954 8_2:-61065584 None:intergenic
TTATGATTGCGGAGGTGGAT+CGG 0.487731 8_2:-61064562 None:intergenic
CAAGACTATGCCTGGTCCAT+AGG 0.490752 8_2:-61067017 None:intergenic
GAGTCTACTTGATCTTCTGC+AGG 0.492839 8_2:+61066414 Msa1259410:CDS
TTCACTTTGCTTGTTTCCAA+AGG 0.493034 8_2:-61066166 None:intergenic
GATAGCAACTTAGATAAATA+CGG 0.496058 8_2:-61067552 None:intergenic
CAAAACAAAAGGGCGGTGAG+AGG 0.496670 8_2:-61067581 None:intergenic
GGAATCGCCGGCAACATCTC+CGG 0.497413 8_2:-61064537 None:intergenic
ATATGACACTATTGTAAAAG+AGG 0.498872 8_2:+61067448 Msa1259410:three_prime_UTR
TCTCAAAATTCATGTAATCC+AGG 0.499696 8_2:+61065926 Msa1259410:CDS
ATTCTTCAACGAAGTGTGAC+CGG 0.501369 8_2:-61065229 None:intergenic
AAATCACAATCTTCTTCTAA+AGG 0.501812 8_2:+61065593 Msa1259410:CDS
GAAAGCTGCGGCGGAGACGC+TGG 0.503998 8_2:+61065302 Msa1259410:CDS
TGAAGTTATGAATCAGATGT+TGG 0.505651 8_2:+61065520 Msa1259410:CDS
AGATTCGCCATTACTGAACC+TGG 0.505669 8_2:-61065944 None:intergenic
GATGAGTTCTGTGGCGGCAA+GGG 0.505954 8_2:-61064704 None:intergenic
ACTATATTATATAGAGTGCC+AGG 0.506674 8_2:+61067490 Msa1259410:three_prime_UTR
AAAACAAAAGGGCGGTGAGA+GGG 0.508041 8_2:-61067580 None:intergenic
GGAGTCGGTGGTTACGGTGG+AGG 0.508645 8_2:-61064590 None:intergenic
CAGAGGTACACACTAATTCA+AGG 0.508694 8_2:+61066948 Msa1259410:CDS
GTTATCAGAAGCTCTGTTCA+CGG 0.509351 8_2:+61065014 Msa1259410:CDS
ACATGGCCTTGAGTTGGCAT+TGG 0.514122 8_2:+61066655 Msa1259410:CDS
CCTGCCAGCTGTTTGGTGCA+TGG 0.518138 8_2:-61066858 None:intergenic
AATCAACATTTCTCTGGTCA+AGG 0.520569 8_2:-61067215 None:intergenic
CCGGCGTTATTGACGCTGAT+CGG 0.523665 8_2:+61065180 Msa1259410:CDS
TCTTTAGAGACCCGTGTACA+TGG 0.524010 8_2:+61066638 Msa1259410:CDS
GAGTTGAATGATGCATCAAT+TGG 0.524050 8_2:-61067081 None:intergenic
ACCGCAGAACGTAAAACAAA+AGG 0.524333 8_2:+61064639 Msa1259410:CDS
GCGAGGATCTTCGAGAGGTA+AGG 0.524463 8_2:-61064874 None:intergenic
ATCGGAAGTGTTGTTGAAGC+TGG 0.525821 8_2:+61065198 Msa1259410:CDS
GATAGTGCCAAGACTATGCC+TGG 0.527497 8_2:-61067025 None:intergenic
CAACATCTCCGGTGAGAAAG+TGG 0.527557 8_2:-61064526 None:intergenic
GGCTCAGAGAATGCAAGTGA+TGG 0.530432 8_2:+61065887 Msa1259410:intron
GTGGCAAGATAATGCAGAAA+CGG 0.533307 8_2:-61064956 None:intergenic
TTCTGATAACGGTTTCAGAA+AGG 0.534166 8_2:-61065001 None:intergenic
GGTTTCAGAAAGGTATAAAA+CGG 0.534530 8_2:-61064991 None:intergenic
CATTTCCAACCAAAACAAAA+GGG 0.535172 8_2:-61067591 None:intergenic
TCCTGACTCTCTTCATGATA+CGG 0.537740 8_2:-61066283 None:intergenic
TGTCTTGCTTCCGCGATCGA+CGG 0.538457 8_2:+61065072 Msa1259410:CDS
GCTACCATGCACCAAACAGC+TGG 0.539989 8_2:+61066854 Msa1259410:CDS
TCTTTGGAAACATTATTGAC+AGG 0.543816 8_2:-61066313 None:intergenic
AAAACGGTTGCTTCGTGACG+TGG 0.548441 8_2:-61064975 None:intergenic
CGGAATTCACACACGCGGAT+CGG 0.552405 8_2:-61064936 None:intergenic
TGAATCAGATGTTGGAAGCA+TGG 0.553306 8_2:+61065528 Msa1259410:CDS
TAGTGATATACCATAATGAC+AGG 0.555053 8_2:-61067676 None:intergenic
AGCCAGAGATTCAGGCTCGA+TGG 0.555726 8_2:+61066902 Msa1259410:CDS
CCATGCACCAAACAGCTGGC+AGG 0.559148 8_2:+61066858 Msa1259410:CDS
AAACAAAAGGGCGGTGAGAG+GGG 0.559673 8_2:-61067579 None:intergenic
TGATGAGTTCTGTGGCGGCA+AGG 0.561237 8_2:-61064705 None:intergenic
TATCACTAATCCTCTGGCAG+AGG 0.562174 8_2:+61066931 Msa1259410:CDS
GCCGGAGTCGGTGGTTACGG+TGG 0.562226 8_2:-61064593 None:intergenic
GGTGAAGGGGAGCCTGAAAG+AGG 0.563399 8_2:-61067719 None:intergenic
ATCCGGAGCTCCGTCGATCG+CGG 0.564038 8_2:-61065082 None:intergenic
TGAACGCATTGCTTCCGTAC+CGG 0.564782 8_2:-61064808 None:intergenic
CCTCGACACAACCACCGTAC+CGG 0.564949 8_2:+61064741 Msa1259410:CDS
GGTTGTGAAGGTGCCGGAGT+CGG 0.565007 8_2:-61064605 None:intergenic
AGATTTATCGGAAGCGTCGG+TGG 0.568710 8_2:-61064785 None:intergenic
TCATAGCCAATCTATAAAGA+AGG 0.569844 8_2:+61066983 Msa1259410:three_prime_UTR
GCGTGGGAGTTATGTGAGAA+CGG 0.571788 8_2:-61064494 None:intergenic
CTATGGACCAGGCATAGTCT+TGG 0.572278 8_2:+61067018 Msa1259410:three_prime_UTR
AGTCTTGGCACTATCACCCA+TGG 0.574191 8_2:+61067033 Msa1259410:three_prime_UTR
ATCACAAAGATGCATTCCCA+TGG 0.576419 8_2:-61067050 None:intergenic
TTAGATAAATACGGAGTACA+TGG 0.578157 8_2:-61067543 None:intergenic
ATCAACATTTCTCTGGTCAA+GGG 0.579894 8_2:-61067214 None:intergenic
CCAACTCAAGGCCATGTACA+CGG 0.583283 8_2:-61066649 None:intergenic
CTCAACGATTCCACCAAACA+CGG 0.583409 8_2:-61065258 None:intergenic
CGTGAAGACGAAGATGCAAA+CGG 0.584101 8_2:-61064450 None:intergenic
ACATTCTTACAGAAATTCAT+CGG 0.584524 8_2:+61066593 Msa1259410:intron
TATGACACTATTGTAAAAGA+GGG 0.587661 8_2:+61067449 Msa1259410:three_prime_UTR
TGGAGGAGTCTTTATGATTG+CGG 0.589386 8_2:-61064573 None:intergenic
CAACTCAAGGCCATGTACAC+GGG 0.590691 8_2:-61066648 None:intergenic
GCTCAGAGAATGCAAGTGAT+GGG 0.590818 8_2:+61065888 Msa1259410:intron
CGACGCTTCCGATAAATCTC+CGG 0.590939 8_2:+61064789 Msa1259410:CDS
GGCGGTGAGGAAAGCTGCGG+CGG 0.592263 8_2:+61065293 Msa1259410:CDS
ATGTAATCCAGGTTCAGTAA+TGG 0.594185 8_2:+61065937 Msa1259410:CDS
TGTCTTGTTAATGCTGAAGA+AGG 0.596709 8_2:+61067336 Msa1259410:three_prime_UTR
AAAACAAACCACTTTCTCAC+CGG 0.598721 8_2:+61064518 Msa1259410:five_prime_UTR
TCCACCGTAACCACCGACTC+CGG 0.599380 8_2:+61064592 Msa1259410:five_prime_UTR
GAATTTGGTTCCGTGTTTGG+TGG 0.601791 8_2:+61065248 Msa1259410:CDS
GAAGAAGATTGTGATTTCGG+AGG 0.604897 8_2:-61065587 None:intergenic
AAAGAATTGGGATGTTCAAG+TGG 0.606059 8_2:+61066096 Msa1259410:CDS
CGGTGGAGTACATGCAAGAG+AGG 0.606172 8_2:-61064768 None:intergenic
TGGTGCATGGTAGCAAGTGA+TGG 0.606370 8_2:-61066845 None:intergenic
CGGAGATTTATCGGAAGCGT+CGG 0.606842 8_2:-61064788 None:intergenic
CCTTAAGTAGTAATAAGAGA+AGG 0.608938 8_2:+61066044 Msa1259410:CDS
AGCTGCGGCGGAGACGCTGG+TGG 0.609203 8_2:+61065305 Msa1259410:CDS
GAGTTCACCCTGATCAGCCA+TGG 0.611946 8_2:-61066119 None:intergenic
TTGGGATGTTCAAGTGGCCA+TGG 0.613475 8_2:+61066102 Msa1259410:CDS
AAAGGGCGGTGAGAGGGGAA+GGG 0.614806 8_2:-61067574 None:intergenic
GTGAAGGGGAGCCTGAAAGA+GGG 0.617163 8_2:-61067718 None:intergenic
TGTGAAGGTGCCGGAGTCGG+TGG 0.618376 8_2:-61064602 None:intergenic
GTTCTGCGGTGACGACATTG+TGG 0.618381 8_2:-61064626 None:intergenic
TATCATCCAATGCCAACTCA+AGG 0.619787 8_2:-61066661 None:intergenic
GTCTTGGCACTATCACCCAT+GGG 0.619828 8_2:+61067034 Msa1259410:three_prime_UTR
CATTGTGGTTGTGAAGGTGC+CGG 0.625292 8_2:-61064611 None:intergenic
ATCAGGGTGAACTCCAGGAG+AGG 0.625370 8_2:+61066128 Msa1259410:CDS
GGATTACTCTCCGAAACTCA+CGG 0.627716 8_2:+61064838 Msa1259410:CDS
AAGGGAGTGAGTGTCGCGGT+CGG 0.628539 8_2:-61064686 None:intergenic
CCGGTACGGTGGTTGTGTCG+AGG 0.630766 8_2:-61064741 None:intergenic
CCGTGTACATGGCCTTGAGT+TGG 0.633390 8_2:+61066649 Msa1259410:CDS
GATTGCGGAGGTGGATCGGG+AGG 0.636299 8_2:-61064558 None:intergenic
CAAGTGGCCATGGCTGATCA+GGG 0.636395 8_2:+61066112 Msa1259410:CDS
CAGTAATGGCGAATCTAAGG+AGG 0.638842 8_2:+61065951 Msa1259410:CDS
GGAAGTGTTGTTGAAGCTGG+TGG 0.639352 8_2:+61065201 Msa1259410:CDS
TGACGACATTGTGGTTGTGA+AGG 0.639933 8_2:-61064617 None:intergenic
TCAGGGTGAACTCCAGGAGA+GGG 0.640702 8_2:+61066129 Msa1259410:CDS
CCGATCAGCGTCAATAACGC+CGG 0.640780 8_2:-61065180 None:intergenic
AGGAGTCTTTATGATTGCGG+AGG 0.642732 8_2:-61064570 None:intergenic
CAGAGATTCAGGCTCGATGG+AGG 0.644429 8_2:+61066905 Msa1259410:CDS
TCACAAAGATGCATTCCCAT+GGG 0.647435 8_2:-61067049 None:intergenic
ATTTGGCTGTATCAAATGGA+AGG 0.647861 8_2:+61066693 Msa1259410:CDS
TGAGAATCGACGATTTGACA+AGG 0.648989 8_2:+61065389 Msa1259410:CDS
CTGCATTTCGTACTTTGTCG+CGG 0.650646 8_2:+61067131 Msa1259410:three_prime_UTR
TAAACTCTATCAAGGTGAAG+GGG 0.655350 8_2:-61067732 None:intergenic
AAAGCATGGTGGATCTCGTG+TGG 0.659084 8_2:+61066258 Msa1259410:CDS
CTTCCGATAAATCTCCGGTA+CGG 0.659557 8_2:+61064794 Msa1259410:CDS
AAGAGGGTAGAAATCTGTCA+AGG 0.663112 8_2:-61067702 None:intergenic
GCAAGAGAGGAAAACCGGTA+CGG 0.663240 8_2:-61064755 None:intergenic
GATAGTTCTACTGCTAGCAA+TGG 0.663534 8_2:+61066007 Msa1259410:CDS
CGTTGGCGAGGATCTTCGAG+AGG 0.666195 8_2:-61064879 None:intergenic
GCAATCGTGATTGGGCGGTG+AGG 0.672486 8_2:+61065280 Msa1259410:CDS
CTAGCAGTAGAACTATCTGG+TGG 0.675785 8_2:-61066001 None:intergenic
CAGAGAATGCAAGTGATGGG+CGG 0.677967 8_2:+61065891 Msa1259410:intron
TCAGCATTAACAAGACAACA+TGG 0.681390 8_2:-61067330 None:intergenic
TTCCAACCAAAACAAAAGGG+CGG 0.683986 8_2:-61067588 None:intergenic
TTAGTGTGTACCTCTGCCAG+AGG 0.685485 8_2:-61066941 None:intergenic
GTTGAGCAATCGTGATTGGG+CGG 0.693426 8_2:+61065275 Msa1259410:CDS
AGCAAGCAACATGAATTACG+AGG 0.696945 8_2:-61066734 None:intergenic
ACGGAGGCGACGAATTACGT+TGG 0.697544 8_2:-61064896 None:intergenic
AGAGAGGAAAACCGGTACGG+TGG 0.698045 8_2:-61064752 None:intergenic
CGGCAAGGGAGTGAGTGTCG+CGG 0.698773 8_2:-61064690 None:intergenic
AAATTCTGGAGGAAAACACA+AGG 0.704372 8_2:+61066782 Msa1259410:CDS
TTGGGCGGTGAGGAAAGCTG+CGG 0.707091 8_2:+61065290 Msa1259410:CDS
AATCGTGATGAGTTCTGTGG+CGG 0.713901 8_2:-61064710 None:intergenic
CCACCACGTCGAGAGAACTG+CGG 0.713909 8_2:-61066818 None:intergenic
TGCAATCGTGATGAGTTCTG+TGG 0.715694 8_2:-61064713 None:intergenic
GCAAGCAACATGAATTACGA+GGG 0.715714 8_2:-61066733 None:intergenic
AGAAACGGAATTCACACACG+CGG 0.769480 8_2:-61064941 None:intergenic
ACCACGTCGAGAGAACTGCG+GGG 0.836426 8_2:-61066816 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGAAATTATGATAAATAG+AGG + chr8_2:61065742-61065761 Msa1259410:intron 15.0%
!! TAGAAATTATGATAAATAGA+GGG + chr8_2:61065743-61065762 Msa1259410:intron 15.0%
!! AAAATTAAACCAAAAGAATT+GGG + chr8_2:61066084-61066103 Msa1259410:CDS 15.0%
!! AATGCAGTAATAAAAAAAAA+AGG - chr8_2:61067118-61067137 None:intergenic 15.0%
!! TGAGTAGAGTAAAAAAAAAA+AGG + chr8_2:61064355-61064374 Msa1259410:five_prime_UTR 20.0%
!! TTTGTTCTTGTGTTTATTTA+TGG + chr8_2:61065436-61065455 Msa1259410:intron 20.0%
!! GAAAATTAAACCAAAAGAAT+TGG + chr8_2:61066083-61066102 Msa1259410:CDS 20.0%
!! AATGTTCATCATTAAAAGAT+TGG - chr8_2:61066566-61066585 None:intergenic 20.0%
! AAAAATCATCAGAAACATCA+GGG - chr8_2:61065562-61065581 None:intergenic 25.0%
! TTAGAAGAAGATTGTGATTT+CGG - chr8_2:61065593-61065612 None:intergenic 25.0%
! AAATCACAATCTTCTTCTAA+AGG + chr8_2:61065593-61065612 Msa1259410:CDS 25.0%
!! CTAAAGGTAGCATTAAATTT+TGG + chr8_2:61065609-61065628 Msa1259410:intron 25.0%
! GATGATAGAATAAAAAAGCA+TGG + chr8_2:61066244-61066263 Msa1259410:CDS 25.0%
! GTCATTCTTAAAAAGATCTT+TGG - chr8_2:61066332-61066351 None:intergenic 25.0%
! GGAGATAAAAAAAAGTAACT+TGG - chr8_2:61066459-61066478 None:intergenic 25.0%
!!! TGTTTTTGTCTGCAAATTTT+TGG + chr8_2:61066481-61066500 Msa1259410:intron 25.0%
! ACATTCTTACAGAAATTCAT+CGG + chr8_2:61066593-61066612 Msa1259410:intron 25.0%
!!! ATTTTTTGAGCTCTAAATTC+TGG + chr8_2:61066768-61066787 Msa1259410:CDS 25.0%
! ATATGACACTATTGTAAAAG+AGG + chr8_2:61067448-61067467 Msa1259410:three_prime_UTR 25.0%
! TATGACACTATTGTAAAAGA+GGG + chr8_2:61067449-61067468 Msa1259410:three_prime_UTR 25.0%
! GATAGCAACTTAGATAAATA+CGG - chr8_2:61067555-61067574 None:intergenic 25.0%
! GGTTTCAGAAAGGTATAAAA+CGG - chr8_2:61064994-61065013 None:intergenic 30.0%
! TCTTAAGCGTGAAGTATTTA+AGG + chr8_2:61065152-61065171 Msa1259410:CDS 30.0%
! GATTGAGATTGTGTTTGTTT+TGG + chr8_2:61065478-61065497 Msa1259410:intron 30.0%
!!! GATTGTGTTTGTTTTGGTTT+AGG + chr8_2:61065484-61065503 Msa1259410:intron 30.0%
TGAAGTTATGAATCAGATGT+TGG + chr8_2:61065520-61065539 Msa1259410:CDS 30.0%
GAAAAATCATCAGAAACATC+AGG - chr8_2:61065563-61065582 None:intergenic 30.0%
!!! ACAATCTGGCTTTCTATTTT+TGG - chr8_2:61065698-61065717 None:intergenic 30.0%
GACATTAAACAAACACAATC+TGG - chr8_2:61065712-61065731 None:intergenic 30.0%
! TTGTGATCTATTGCATTGAT+TGG + chr8_2:61065823-61065842 Msa1259410:intron 30.0%
! TTTTTCTGCGTTTGTGTTTT+TGG + chr8_2:61065866-61065885 Msa1259410:intron 30.0%
!!! TTACATGAATTTTGAGACAC+AGG - chr8_2:61065923-61065942 None:intergenic 30.0%
TCTCAAAATTCATGTAATCC+AGG + chr8_2:61065926-61065945 Msa1259410:CDS 30.0%
CCTTCTCTTATTACTACTTA+AGG - chr8_2:61066047-61066066 None:intergenic 30.0%
CCTTAAGTAGTAATAAGAGA+AGG + chr8_2:61066044-61066063 Msa1259410:CDS 30.0%
TAGTAATAAGAGAAGGAGTT+TGG + chr8_2:61066051-61066070 Msa1259410:CDS 30.0%
AGTAATAAGAGAAGGAGTTT+GGG + chr8_2:61066052-61066071 Msa1259410:CDS 30.0%
! GATAGAATAAAAAAGCATGG+TGG + chr8_2:61066247-61066266 Msa1259410:CDS 30.0%
! TCTTTGGAAACATTATTGAC+AGG - chr8_2:61066316-61066335 None:intergenic 30.0%
AAATTTGCAGACAAAAACAG+TGG - chr8_2:61066480-61066499 None:intergenic 30.0%
!! TTTGTCTGCAAATTTTTGGA+AGG + chr8_2:61066485-61066504 Msa1259410:intron 30.0%
GATGAATTTCTGTAAGAATG+TGG - chr8_2:61066594-61066613 None:intergenic 30.0%
GGATGATATCTCTTATGATT+TGG + chr8_2:61066676-61066695 Msa1259410:CDS 30.0%
! TATGATTTGGCTGTATCAAA+TGG + chr8_2:61066689-61066708 Msa1259410:CDS 30.0%
TCATAGCCAATCTATAAAGA+AGG + chr8_2:61066983-61067002 Msa1259410:three_prime_UTR 30.0%
! ACTATATTATATAGAGTGCC+AGG + chr8_2:61067490-61067509 Msa1259410:three_prime_UTR 30.0%
TTAGATAAATACGGAGTACA+TGG - chr8_2:61067546-61067565 None:intergenic 30.0%
CATTTCCAACCAAAACAAAA+GGG - chr8_2:61067594-61067613 None:intergenic 30.0%
TCATTTCCAACCAAAACAAA+AGG - chr8_2:61067595-61067614 None:intergenic 30.0%
TAGTGATATACCATAATGAC+AGG - chr8_2:61067679-61067698 None:intergenic 30.0%
! TTTTTACTCTACTCAAGAGC+TGG - chr8_2:61064350-61064369 None:intergenic 35.0%
!!! ACTGCACTGTCGTTTTATTT+GGG - chr8_2:61064415-61064434 None:intergenic 35.0%
!!! AACTGCACTGTCGTTTTATT+TGG - chr8_2:61064416-61064435 None:intergenic 35.0%
!!! AAAGTGGTTTGTTTTTGCGT+GGG - chr8_2:61064513-61064532 None:intergenic 35.0%
AAAACAAACCACTTTCTCAC+CGG + chr8_2:61064518-61064537 Msa1259410:five_prime_UTR 35.0%
!!! ACCTTTTGTTTTACGTTCTG+CGG - chr8_2:61064643-61064662 None:intergenic 35.0%
!! TTCTGATAACGGTTTCAGAA+AGG - chr8_2:61065004-61065023 None:intergenic 35.0%
!! TTAAGAAGCTTCTGGAACTT+CGG - chr8_2:61065138-61065157 None:intergenic 35.0%
! TGAAGTATTTAAGGCGAAAC+CGG + chr8_2:61065161-61065180 Msa1259410:CDS 35.0%
TCACACTTCGTTGAAGAATT+TGG + chr8_2:61065233-61065252 Msa1259410:CDS 35.0%
GGATTTCTTATCGGAATTCA+AGG + chr8_2:61065347-61065366 Msa1259410:CDS 35.0%
!!! ATGAATTTTGAGACACAGGA+GGG - chr8_2:61065919-61065938 None:intergenic 35.0%
ATGTAATCCAGGTTCAGTAA+TGG + chr8_2:61065937-61065956 Msa1259410:CDS 35.0%
! CTTGAACATCCCAATTCTTT+TGG - chr8_2:61066096-61066115 None:intergenic 35.0%
AAAGAATTGGGATGTTCAAG+TGG + chr8_2:61066096-61066115 Msa1259410:CDS 35.0%
TTCACTTTGCTTGTTTCCAA+AGG - chr8_2:61066169-61066188 None:intergenic 35.0%
AATAGTGCTCGATTCATTTC+TGG - chr8_2:61066211-61066230 None:intergenic 35.0%
! AGATTGGTTCATGCATGTAA+AGG - chr8_2:61066550-61066569 None:intergenic 35.0%
GAATTTCTGTAAGAATGTGG+AGG - chr8_2:61066591-61066610 None:intergenic 35.0%
ATTTGGCTGTATCAAATGGA+AGG + chr8_2:61066693-61066712 Msa1259410:CDS 35.0%
!!! TTTTGAGCTCTAAATTCTGG+AGG + chr8_2:61066771-61066790 Msa1259410:CDS 35.0%
! AAATTCTGGAGGAAAACACA+AGG + chr8_2:61066782-61066801 Msa1259410:CDS 35.0%
! ATTTTACAAGCCAGAGATTC+AGG + chr8_2:61066894-61066913 Msa1259410:CDS 35.0%
!! AGGTTTTATCACTAATCCTC+TGG + chr8_2:61066925-61066944 Msa1259410:CDS 35.0%
CGTGAACCTTCTTTATAGAT+TGG - chr8_2:61066992-61067011 None:intergenic 35.0%
GAGTTGAATGATGCATCAAT+TGG - chr8_2:61067084-61067103 None:intergenic 35.0%
! TCATCAGTTGATTCTCAATC+AGG + chr8_2:61067182-61067201 Msa1259410:three_prime_UTR 35.0%
ATCAACATTTCTCTGGTCAA+GGG - chr8_2:61067217-61067236 None:intergenic 35.0%
AATCAACATTTCTCTGGTCA+AGG - chr8_2:61067218-61067237 None:intergenic 35.0%
TCGATCAATCAACATTTCTC+TGG - chr8_2:61067224-61067243 None:intergenic 35.0%
TCAGCATTAACAAGACAACA+TGG - chr8_2:61067333-61067352 None:intergenic 35.0%
! TGTCTTGTTAATGCTGAAGA+AGG + chr8_2:61067336-61067355 Msa1259410:three_prime_UTR 35.0%
GATCATTGGACAAGAATGAT+TGG + chr8_2:61067408-61067427 Msa1259410:three_prime_UTR 35.0%
TTTAATGCAAAAAAGCAGCC+TGG - chr8_2:61067511-61067530 None:intergenic 35.0%
!!! TGTTTTGGTTGGAAATGAAG+TGG + chr8_2:61067597-61067616 Msa1259410:three_prime_UTR 35.0%
!!! GTTTTGGTTGGAAATGAAGT+GGG + chr8_2:61067598-61067617 Msa1259410:three_prime_UTR 35.0%
TCTATATGTGCCTGTCATTA+TGG + chr8_2:61067666-61067685 Msa1259410:three_prime_UTR 35.0%
!!! GAAAGTGGTTTGTTTTTGCG+TGG - chr8_2:61064514-61064533 None:intergenic 40.0%
TGGAGGAGTCTTTATGATTG+CGG - chr8_2:61064576-61064595 None:intergenic 40.0%
ACCGCAGAACGTAAAACAAA+AGG + chr8_2:61064639-61064658 Msa1259410:CDS 40.0%
TACATGCAAGAGAGGAAAAC+CGG - chr8_2:61064763-61064782 None:intergenic 40.0%
! AAGCAATGCGTTCAGCTTTT+CGG + chr8_2:61064817-61064836 Msa1259410:CDS 40.0%
GTGGCAAGATAATGCAGAAA+CGG - chr8_2:61064959-61064978 None:intergenic 40.0%
GTGAACAGAGCTTCTGATAA+CGG - chr8_2:61065015-61065034 None:intergenic 40.0%
GTTATCAGAAGCTCTGTTCA+CGG + chr8_2:61065014-61065033 Msa1259410:CDS 40.0%
TTTGCTAATCTAGCAGGTTC+CGG - chr8_2:61065108-61065127 None:intergenic 40.0%
AACAGCTTTGCTAATCTAGC+AGG - chr8_2:61065114-61065133 None:intergenic 40.0%
ATTCTTCAACGAAGTGTGAC+CGG - chr8_2:61065232-61065251 None:intergenic 40.0%
! GAAGAATTTGGTTCCGTGTT+TGG + chr8_2:61065245-61065264 Msa1259410:CDS 40.0%
AATCGTTGAGCAATCGTGAT+TGG + chr8_2:61065271-61065290 Msa1259410:CDS 40.0%
ATCGTTGAGCAATCGTGATT+GGG + chr8_2:61065272-61065291 Msa1259410:CDS 40.0%
TGAGAATCGACGATTTGACA+AGG + chr8_2:61065389-61065408 Msa1259410:CDS 40.0%
TGAATCAGATGTTGGAAGCA+TGG + chr8_2:61065528-61065547 Msa1259410:CDS 40.0%
GAAGAAGATTGTGATTTCGG+AGG - chr8_2:61065590-61065609 None:intergenic 40.0%
TAGATCACAAACAGACGCAT+TGG - chr8_2:61065813-61065832 None:intergenic 40.0%
!!! CATGAATTTTGAGACACAGG+AGG - chr8_2:61065920-61065939 None:intergenic 40.0%
GTTCAGTAATGGCGAATCTA+AGG + chr8_2:61065948-61065967 Msa1259410:CDS 40.0%
!! GGTGGACTGAATCTATTAAC+AGG - chr8_2:61065986-61066005 None:intergenic 40.0%
TTGCTAGCAGTAGAACTATC+TGG - chr8_2:61066007-61066026 None:intergenic 40.0%
! GATAGTTCTACTGCTAGCAA+TGG + chr8_2:61066007-61066026 Msa1259410:CDS 40.0%
GGATGAAAATGGAACTCCTT+TGG + chr8_2:61066150-61066169 Msa1259410:CDS 40.0%
TCCTGACTCTCTTCATGATA+CGG - chr8_2:61066286-61066305 None:intergenic 40.0%
TTCATCGGAAGCTCACAAAA+TGG + chr8_2:61066608-61066627 Msa1259410:CDS 40.0%
TATCATCCAATGCCAACTCA+AGG - chr8_2:61066664-61066683 None:intergenic 40.0%
GCAAGCAACATGAATTACGA+GGG - chr8_2:61066736-61066755 None:intergenic 40.0%
AGCAAGCAACATGAATTACG+AGG - chr8_2:61066737-61066756 None:intergenic 40.0%
! AATTCATGTTGCTTGCTACC+TGG + chr8_2:61066740-61066759 Msa1259410:CDS 40.0%
! CAGAGGTACACACTAATTCA+AGG + chr8_2:61066948-61066967 Msa1259410:CDS 40.0%
! GAAGGTTCACGTTTTACCTA+TGG + chr8_2:61067001-61067020 Msa1259410:three_prime_UTR 40.0%
TCACAAAGATGCATTCCCAT+GGG - chr8_2:61067052-61067071 None:intergenic 40.0%
ATCACAAAGATGCATTCCCA+TGG - chr8_2:61067053-61067072 None:intergenic 40.0%
TGCTGCACAATTTAGAAAGC+AGG + chr8_2:61067157-61067176 Msa1259410:three_prime_UTR 40.0%
! GAATGATTGGAGTGCTTTCA+CGG + chr8_2:61067421-61067440 Msa1259410:three_prime_UTR 40.0%
TTCCAACCAAAACAAAAGGG+CGG - chr8_2:61067591-61067610 None:intergenic 40.0%
AAGAGGGTAGAAATCTGTCA+AGG - chr8_2:61067705-61067724 None:intergenic 40.0%
ACAGATTTCTACCCTCTTTC+AGG + chr8_2:61067707-61067726 Msa1259410:three_prime_UTR 40.0%
!! GCTGGTTGGGTTTGTTTTCT+TGG - chr8_2:61064332-61064351 None:intergenic 45.0%
ACTCTACTCAAGAGCTGGTT+GGG - chr8_2:61064345-61064364 None:intergenic 45.0%
TACTCTACTCAAGAGCTGGT+TGG - chr8_2:61064346-61064365 None:intergenic 45.0%
CGTGAAGACGAAGATGCAAA+CGG - chr8_2:61064453-61064472 None:intergenic 45.0%
TTATGATTGCGGAGGTGGAT+CGG - chr8_2:61064565-61064584 None:intergenic 45.0%
AGTCTTTATGATTGCGGAGG+TGG - chr8_2:61064570-61064589 None:intergenic 45.0%
AGGAGTCTTTATGATTGCGG+AGG - chr8_2:61064573-61064592 None:intergenic 45.0%
TGACGACATTGTGGTTGTGA+AGG - chr8_2:61064620-61064639 None:intergenic 45.0%
AATCGTGATGAGTTCTGTGG+CGG - chr8_2:61064713-61064732 None:intergenic 45.0%
TGCAATCGTGATGAGTTCTG+TGG - chr8_2:61064716-61064735 None:intergenic 45.0%
CTTCCGATAAATCTCCGGTA+CGG + chr8_2:61064794-61064813 Msa1259410:CDS 45.0%
CTTCCGTACCGGAGATTTAT+CGG - chr8_2:61064800-61064819 None:intergenic 45.0%
GGATTACTCTCCGAAACTCA+CGG + chr8_2:61064838-61064857 Msa1259410:CDS 45.0%
AGAAACGGAATTCACACACG+CGG - chr8_2:61064944-61064963 None:intergenic 45.0%
CAAGAACAGAACGCGCAAAT+TGG + chr8_2:61065039-61065058 Msa1259410:CDS 45.0%
CTTCACGCTTAAGAAGCTTC+TGG - chr8_2:61065146-61065165 None:intergenic 45.0%
!! ATCGGAAGTGTTGTTGAAGC+TGG + chr8_2:61065198-61065217 Msa1259410:CDS 45.0%
! GAATTTGGTTCCGTGTTTGG+TGG + chr8_2:61065248-61065267 Msa1259410:CDS 45.0%
CTCAACGATTCCACCAAACA+CGG - chr8_2:61065261-61065280 None:intergenic 45.0%
CGTGGAGAGGGATTTCTTAT+CGG + chr8_2:61065338-61065357 Msa1259410:CDS 45.0%
GAAGATTGTGATTTCGGAGG+TGG - chr8_2:61065587-61065606 None:intergenic 45.0%
GCTCAGAGAATGCAAGTGAT+GGG + chr8_2:61065888-61065907 Msa1259410:intron 45.0%
AGATTCGCCATTACTGAACC+TGG - chr8_2:61065947-61065966 None:intergenic 45.0%
CAGTAATGGCGAATCTAAGG+AGG + chr8_2:61065951-61065970 Msa1259410:CDS 45.0%
CTAGCAGTAGAACTATCTGG+TGG - chr8_2:61066004-61066023 None:intergenic 45.0%
! TTCCATTTTCATCCCTCTCC+TGG - chr8_2:61066144-61066163 None:intergenic 45.0%
TCCGTATCATGAAGAGAGTC+AGG + chr8_2:61066282-61066301 Msa1259410:CDS 45.0%
!! CGATTTGTTGCAACTGGTTG+CGG - chr8_2:61066381-61066400 None:intergenic 45.0%
!! GCTTCTCGATTTGTTGCAAC+TGG - chr8_2:61066387-61066406 None:intergenic 45.0%
GAGTCTACTTGATCTTCTGC+AGG + chr8_2:61066414-61066433 Msa1259410:CDS 45.0%
! TCTTTAGAGACCCGTGTACA+TGG + chr8_2:61066638-61066657 Msa1259410:CDS 45.0%
GAGCTCAAAAAATCTGCTCC+AGG - chr8_2:61066761-61066780 None:intergenic 45.0%
AGAACTGCGGGGATAAATAC+TGG - chr8_2:61066808-61066827 None:intergenic 45.0%
TATCACTAATCCTCTGGCAG+AGG + chr8_2:61066931-61066950 Msa1259410:CDS 45.0%
! TCACGTTTTACCTATGGACC+AGG + chr8_2:61067007-61067026 Msa1259410:three_prime_UTR 45.0%
CTGCATTTCGTACTTTGTCG+CGG + chr8_2:61067131-61067150 Msa1259410:three_prime_UTR 45.0%
ATGATCATCGTGCGCAAGTA+AGG - chr8_2:61067394-61067413 None:intergenic 45.0%
TACTTGCGCACGATGATCAT+TGG + chr8_2:61067394-61067413 Msa1259410:three_prime_UTR 45.0%
AAAACAAAAGGGCGGTGAGA+GGG - chr8_2:61067583-61067602 None:intergenic 45.0%
GCGTGGGAGTTATGTGAGAA+CGG - chr8_2:61064497-61064516 None:intergenic 50.0%
CAACATCTCCGGTGAGAAAG+TGG - chr8_2:61064529-61064548 None:intergenic 50.0%
TTTCTCACCGGAGATGTTGC+CGG + chr8_2:61064530-61064549 Msa1259410:five_prime_UTR 50.0%
TATGATTGCGGAGGTGGATC+GGG - chr8_2:61064564-61064583 None:intergenic 50.0%
! CATTGTGGTTGTGAAGGTGC+CGG - chr8_2:61064614-61064633 None:intergenic 50.0%
GCAAGAGAGGAAAACCGGTA+CGG - chr8_2:61064758-61064777 None:intergenic 50.0%
!! AGATTTATCGGAAGCGTCGG+TGG - chr8_2:61064788-61064807 None:intergenic 50.0%
! CGGAGATTTATCGGAAGCGT+CGG - chr8_2:61064791-61064810 None:intergenic 50.0%
CGACGCTTCCGATAAATCTC+CGG + chr8_2:61064789-61064808 Msa1259410:CDS 50.0%
TGAACGCATTGCTTCCGTAC+CGG - chr8_2:61064811-61064830 None:intergenic 50.0%
AAGCGCGTTACCGTGAGTTT+CGG - chr8_2:61064851-61064870 None:intergenic 50.0%
AAAACGGTTGCTTCGTGACG+TGG - chr8_2:61064978-61064997 None:intergenic 50.0%
AATCTAGCAGGTTCCGGATC+CGG - chr8_2:61065102-61065121 None:intergenic 50.0%
!! GGAAGTGTTGTTGAAGCTGG+TGG + chr8_2:61065201-61065220 Msa1259410:CDS 50.0%
GTTGAGCAATCGTGATTGGG+CGG + chr8_2:61065275-61065294 Msa1259410:CDS 50.0%
!!! TGGTTTTGGCTAACGTGGAG+AGG + chr8_2:61065325-61065344 Msa1259410:CDS 50.0%
!!! GGTTTTGGCTAACGTGGAGA+GGG + chr8_2:61065326-61065345 Msa1259410:CDS 50.0%
GGCTCAGAGAATGCAAGTGA+TGG + chr8_2:61065887-61065906 Msa1259410:intron 50.0%
! CAGAGAATGCAAGTGATGGG+CGG + chr8_2:61065891-61065910 Msa1259410:intron 50.0%
TTGGGATGTTCAAGTGGCCA+TGG + chr8_2:61066102-61066121 Msa1259410:CDS 50.0%
CTCCAGGAGAGGGATGAAAA+TGG + chr8_2:61066139-61066158 Msa1259410:CDS 50.0%
!! AAAGCATGGTGGATCTCGTG+TGG + chr8_2:61066258-61066277 Msa1259410:CDS 50.0%
CAACTCAAGGCCATGTACAC+GGG - chr8_2:61066651-61066670 None:intergenic 50.0%
CCAACTCAAGGCCATGTACA+CGG - chr8_2:61066652-61066671 None:intergenic 50.0%
!! ACATGGCCTTGAGTTGGCAT+TGG + chr8_2:61066655-61066674 Msa1259410:CDS 50.0%
TGGTGCATGGTAGCAAGTGA+TGG - chr8_2:61066848-61066867 None:intergenic 50.0%
TTAGTGTGTACCTCTGCCAG+AGG - chr8_2:61066944-61066963 None:intergenic 50.0%
CAAGACTATGCCTGGTCCAT+AGG - chr8_2:61067020-61067039 None:intergenic 50.0%
CTATGGACCAGGCATAGTCT+TGG + chr8_2:61067018-61067037 Msa1259410:three_prime_UTR 50.0%
GATAGTGCCAAGACTATGCC+TGG - chr8_2:61067028-61067047 None:intergenic 50.0%
AGTCTTGGCACTATCACCCA+TGG + chr8_2:61067033-61067052 Msa1259410:three_prime_UTR 50.0%
GTCTTGGCACTATCACCCAT+GGG + chr8_2:61067034-61067053 Msa1259410:three_prime_UTR 50.0%
AAACAAAAGGGCGGTGAGAG+GGG - chr8_2:61067582-61067601 None:intergenic 50.0%
CAAAACAAAAGGGCGGTGAG+AGG - chr8_2:61067584-61067603 None:intergenic 50.0%
!! CTCTCACCGCCCTTTTGTTT+TGG + chr8_2:61067582-61067601 Msa1259410:three_prime_UTR 50.0%
!!! CACCGCCCTTTTGTTTTGGT+TGG + chr8_2:61067586-61067605 Msa1259410:three_prime_UTR 50.0%
GTTCTGCGGTGACGACATTG+TGG - chr8_2:61064629-61064648 None:intergenic 55.0%
GATGAGTTCTGTGGCGGCAA+GGG - chr8_2:61064707-61064726 None:intergenic 55.0%
TGATGAGTTCTGTGGCGGCA+AGG - chr8_2:61064708-61064727 None:intergenic 55.0%
AGAGAGGAAAACCGGTACGG+TGG - chr8_2:61064755-61064774 None:intergenic 55.0%
CGGTGGAGTACATGCAAGAG+AGG - chr8_2:61064771-61064790 None:intergenic 55.0%
GCGAGGATCTTCGAGAGGTA+AGG - chr8_2:61064877-61064896 None:intergenic 55.0%
ACGGAGGCGACGAATTACGT+TGG - chr8_2:61064899-61064918 None:intergenic 55.0%
CGGAATTCACACACGCGGAT+CGG - chr8_2:61064939-61064958 None:intergenic 55.0%
TGTCTTGCTTCCGCGATCGA+CGG + chr8_2:61065072-61065091 Msa1259410:CDS 55.0%
CCGATCAGCGTCAATAACGC+CGG - chr8_2:61065183-61065202 None:intergenic 55.0%
CCGGCGTTATTGACGCTGAT+CGG + chr8_2:61065180-61065199 Msa1259410:CDS 55.0%
!!! GCTGGTGGTTTTGGCTAACG+TGG + chr8_2:61065320-61065339 Msa1259410:CDS 55.0%
TCAAGTGGCCATGGCTGATC+AGG + chr8_2:61066111-61066130 Msa1259410:CDS 55.0%
CAAGTGGCCATGGCTGATCA+GGG + chr8_2:61066112-61066131 Msa1259410:CDS 55.0%
GAGTTCACCCTGATCAGCCA+TGG - chr8_2:61066122-61066141 None:intergenic 55.0%
ATCAGGGTGAACTCCAGGAG+AGG + chr8_2:61066128-61066147 Msa1259410:CDS 55.0%
TCAGGGTGAACTCCAGGAGA+GGG + chr8_2:61066129-61066148 Msa1259410:CDS 55.0%
! CCGTGTACATGGCCTTGAGT+TGG + chr8_2:61066649-61066668 Msa1259410:CDS 55.0%
GCTACCATGCACCAAACAGC+TGG + chr8_2:61066854-61066873 Msa1259410:CDS 55.0%
CAGCATTCCTGCCAGCTGTT+TGG - chr8_2:61066868-61066887 None:intergenic 55.0%
AGCCAGAGATTCAGGCTCGA+TGG + chr8_2:61066902-61066921 Msa1259410:CDS 55.0%
CTCCATCGAGCCTGAATCTC+TGG - chr8_2:61066907-61066926 None:intergenic 55.0%
CAGAGATTCAGGCTCGATGG+AGG + chr8_2:61066905-61066924 Msa1259410:CDS 55.0%
TGCTGAAGAAGGCGAGAAGC+TGG + chr8_2:61067347-61067366 Msa1259410:three_prime_UTR 55.0%
GGAATCGCCGGCAACATCTC+CGG - chr8_2:61064540-61064559 None:intergenic 60.0%
!! TCCACCGTAACCACCGACTC+CGG + chr8_2:61064592-61064611 Msa1259410:five_prime_UTR 60.0%
!! GGTTGTGAAGGTGCCGGAGT+CGG - chr8_2:61064608-61064627 None:intergenic 60.0%
AGTGTCGCGGTCGGAGAGTT+TGG - chr8_2:61064680-61064699 None:intergenic 60.0%
! AAGGGAGTGAGTGTCGCGGT+CGG - chr8_2:61064689-61064708 None:intergenic 60.0%
CCTCGACACAACCACCGTAC+CGG + chr8_2:61064741-61064760 Msa1259410:CDS 60.0%
CGTTGGCGAGGATCTTCGAG+AGG - chr8_2:61064882-61064901 None:intergenic 60.0%
GGCGACGAATTACGTTGGCG+AGG - chr8_2:61064894-61064913 None:intergenic 60.0%
!! CCTCCGTGATACCGACTCGT+CGG + chr8_2:61064912-61064931 Msa1259410:CDS 60.0%
GGACCGACGAGTCGGTATCA+CGG - chr8_2:61064918-61064937 None:intergenic 60.0%
GCAATCGTGATTGGGCGGTG+AGG + chr8_2:61065280-61065299 Msa1259410:CDS 60.0%
TTGGGCGGTGAGGAAAGCTG+CGG + chr8_2:61065290-61065309 Msa1259410:CDS 60.0%
GGCTGATCAGGGTGAACTCC+AGG + chr8_2:61066123-61066142 Msa1259410:CDS 60.0%
ACCACGTCGAGAGAACTGCG+GGG - chr8_2:61066819-61066838 None:intergenic 60.0%
CACCACGTCGAGAGAACTGC+GGG - chr8_2:61066820-61066839 None:intergenic 60.0%
CCACCACGTCGAGAGAACTG+CGG - chr8_2:61066821-61066840 None:intergenic 60.0%
!! CCTGCCAGCTGTTTGGTGCA+TGG - chr8_2:61066861-61066880 None:intergenic 60.0%
CCATGCACCAAACAGCTGGC+AGG + chr8_2:61066858-61066877 Msa1259410:CDS 60.0%
AAAGGGCGGTGAGAGGGGAA+GGG - chr8_2:61067577-61067596 None:intergenic 60.0%
AAAAGGGCGGTGAGAGGGGA+AGG - chr8_2:61067578-61067597 None:intergenic 60.0%
GGTGGATCGGGAGGAATCGC+CGG - chr8_2:61064552-61064571 None:intergenic 65.0%
GATTGCGGAGGTGGATCGGG+AGG - chr8_2:61064561-61064580 None:intergenic 65.0%
GGAGTCGGTGGTTACGGTGG+AGG - chr8_2:61064593-61064612 None:intergenic 65.0%
! GGTGCCGGAGTCGGTGGTTA+CGG - chr8_2:61064599-61064618 None:intergenic 65.0%
!! TGTGAAGGTGCCGGAGTCGG+TGG - chr8_2:61064605-61064624 None:intergenic 65.0%
! CGGCAAGGGAGTGAGTGTCG+CGG - chr8_2:61064693-61064712 None:intergenic 65.0%
CCGGTACGGTGGTTGTGTCG+AGG - chr8_2:61064744-61064763 None:intergenic 65.0%
CCGACGAGTCGGTATCACGG+AGG - chr8_2:61064915-61064934 None:intergenic 65.0%
TTCCGCGATCGACGGAGCTC+CGG + chr8_2:61065080-61065099 Msa1259410:CDS 65.0%
ATCCGGAGCTCCGTCGATCG+CGG - chr8_2:61065085-61065104 None:intergenic 65.0%
GATCGACGGAGCTCCGGATC+CGG + chr8_2:61065086-61065105 Msa1259410:CDS 65.0%
! GTTGAAGCTGGTGGCGCGTC+CGG + chr8_2:61065210-61065229 Msa1259410:CDS 65.0%
! GGCGGAGACGCTGGTGGTTT+TGG + chr8_2:61065311-61065330 Msa1259410:CDS 65.0%
TCCCCGCAGTTCTCTCGACG+TGG + chr8_2:61066815-61066834 Msa1259410:CDS 65.0%
CCGCAGTTCTCTCGACGTGG+TGG + chr8_2:61066818-61066837 Msa1259410:CDS 65.0%
GCCGGAGTCGGTGGTTACGG+TGG - chr8_2:61064596-61064615 None:intergenic 70.0%
CGCGGATCGGACCGACGAGT+CGG - chr8_2:61064926-61064945 None:intergenic 70.0%
GGCGGTGAGGAAAGCTGCGG+CGG + chr8_2:61065293-61065312 Msa1259410:CDS 70.0%
GAAAGCTGCGGCGGAGACGC+TGG + chr8_2:61065302-61065321 Msa1259410:CDS 70.0%
AGCTGCGGCGGAGACGCTGG+TGG + chr8_2:61065305-61065324 Msa1259410:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 61064327 61067739 61064327 ID=Msa1259410;Name=Msa1259410
chr8_2 mRNA 61064327 61067739 61064327 ID=Msa1259410-mRNA-1;Parent=Msa1259410;Name=Msa1259410-mRNA-1;_AED=0.00;_eAED=0.00;_QI=304|1|1|1|1|1|4|757|602
chr8_2 exon 61064327 61065410 61064327 ID=Msa1259410-mRNA-1:exon:19165;Parent=Msa1259410-mRNA-1
chr8_2 exon 61065506 61065614 61065506 ID=Msa1259410-mRNA-1:exon:19166;Parent=Msa1259410-mRNA-1
chr8_2 exon 61065894 61066435 61065894 ID=Msa1259410-mRNA-1:exon:19167;Parent=Msa1259410-mRNA-1
chr8_2 exon 61066605 61067739 61066605 ID=Msa1259410-mRNA-1:exon:19168;Parent=Msa1259410-mRNA-1
chr8_2 five_prime_UTR 61064327 61064630 61064327 ID=Msa1259410-mRNA-1:five_prime_utr;Parent=Msa1259410-mRNA-1
chr8_2 CDS 61064631 61065410 61064631 ID=Msa1259410-mRNA-1:cds;Parent=Msa1259410-mRNA-1
chr8_2 CDS 61065506 61065614 61065506 ID=Msa1259410-mRNA-1:cds;Parent=Msa1259410-mRNA-1
chr8_2 CDS 61065894 61066435 61065894 ID=Msa1259410-mRNA-1:cds;Parent=Msa1259410-mRNA-1
chr8_2 CDS 61066605 61066982 61066605 ID=Msa1259410-mRNA-1:cds;Parent=Msa1259410-mRNA-1
chr8_2 three_prime_UTR 61066983 61067739 61066983 ID=Msa1259410-mRNA-1:three_prime_utr;Parent=Msa1259410-mRNA-1
Gene Sequence

>Msa1259410

ATGTCGTCACCGCAGAACGTAAAACAAAAGGTATTCACATGTCTAACCAAACTCTCCGACCGCGACACTCACTCCCTTGCCGCCACAGAACTCATCACGATTGCACGCAACCTCGACACAACCACCGTACCGGTTTTCCTCTCTTGCATGTACTCCACCGACGCTTCCGATAAATCTCCGGTACGGAAGCAATGCGTTCAGCTTTTCGGATTACTCTCCGAAACTCACGGTAACGCGCTTTCTCCTTACCTCTCGAAGATCCTCGCCAACGTAATTCGTCGCCTCCGTGATACCGACTCGTCGGTCCGATCCGCGTGTGTGAATTCCGTTTCTGCATTATCTTGCCACGTCACGAAGCAACCGTTTTATACCTTTCTGAAACCGTTATCAGAAGCTCTGTTCACGGAGCAAGAACAGAACGCGCAAATTGGCGCTGCACTTTGTCTTGCTTCCGCGATCGACGGAGCTCCGGATCCGGAACCTGCTAGATTAGCAAAGCTGTTGCCGAAGTTCCAGAAGCTTCTTAAGCGTGAAGTATTTAAGGCGAAACCGGCGTTATTGACGCTGATCGGAAGTGTTGTTGAAGCTGGTGGCGCGTCCGGTCACACTTCGTTGAAGAATTTGGTTCCGTGTTTGGTGGAATCGTTGAGCAATCGTGATTGGGCGGTGAGGAAAGCTGCGGCGGAGACGCTGGTGGTTTTGGCTAACGTGGAGAGGGATTTCTTATCGGAATTCAAGGCTGATTGCATGAAAGTTTTTGAGAATCGACGATTTGACAAGGTGAAGTTAGTTCGTGAAGTTATGAATCAGATGTTGGAAGCATGGAAGCATATCCCTGATGTTTCTGATGATTTTTCGCCACCTCCGAAATCACAATCTTCTTCTAAAGAGAATGCAAGTGATGGGCGGTACCCTCCTGTGTCTCAAAATTCATGTAATCCAGGTTCAGTAATGGCGAATCTAAGGAGGAGATCAGCTCCTGTTAATAGATTCAGTCCACCAGATAGTTCTACTGCTAGCAATGGCAAGAATGTGAGTTCCTTAAGTAGTAATAAGAGAAGGAGTTTGGGTGTTTCGCGAAAATTAAACCAAAAGAATTGGGATGTTCAAGTGGCCATGGCTGATCAGGGTGAACTCCAGGAGAGGGATGAAAATGGAACTCCTTTGGAAACAAGCAAAGTGAATAAAAGCAAATTTCTAAAGCCAGAAATGAATCGAGCACTATTAAATAAAAATTCTGATGATAGAATAAAAAAGCATGGTGGATCTCGTGTGGTTCCGTATCATGAAGAGAGTCAGGACTCAGTTCCTGTCAATAATGTTTCCAAAGATCTTTTTAAGAATGACAAAGAGAGTGAAGAGTTATCTTTGATCCGCAACCAGTTGCAACAAATCGAGAAGCAACAGTCGAGTCTACTTGATCTTCTGCAGAAATTCATCGGAAGCTCACAAAATGGAATGCGATCTTTAGAGACCCGTGTACATGGCCTTGAGTTGGCATTGGATGATATCTCTTATGATTTGGCTGTATCAAATGGAAGGATGACTAATTCTAATGTCCCTCGTAATTCATGTTGCTTGCTACCTGGAGCAGATTTTTTGAGCTCTAAATTCTGGAGGAAAACACAAGGCCAGTATTTATCCCCGCAGTTCTCTCGACGTGGTGGTGCGCCATCACTTGCTACCATGCACCAAACAGCTGGCAGGAATGCTGAAACTAATTTTACAAGCCAGAGATTCAGGCTCGATGGAGGTTTTATCACTAATCCTCTGGCAGAGGTACACACTAATTCAAGGAACTTTGCTTGA
Protein sequence

>Msa1259410

MSSPQNVKQKVFTCLTKLSDRDTHSLAATELITIARNLDTTTVPVFLSCMYSTDASDKSPVRKQCVQLFGLLSETHGNALSPYLSKILANVIRRLRDTDSSVRSACVNSVSALSCHVTKQPFYTFLKPLSEALFTEQEQNAQIGAALCLASAIDGAPDPEPARLAKLLPKFQKLLKREVFKAKPALLTLIGSVVEAGGASGHTSLKNLVPCLVESLSNRDWAVRKAAAETLVVLANVERDFLSEFKADCMKVFENRRFDKVKLVREVMNQMLEAWKHIPDVSDDFSPPPKSQSSSKENASDGRYPPVSQNSCNPGSVMANLRRRSAPVNRFSPPDSSTASNGKNVSSLSSNKRRSLGVSRKLNQKNWDVQVAMADQGELQERDENGTPLETSKVNKSKFLKPEMNRALLNKNSDDRIKKHGGSRVVPYHEESQDSVPVNNVSKDLFKNDKESEELSLIRNQLQQIEKQQSSLLDLLQKFIGSSQNGMRSLETRVHGLELALDDISYDLAVSNGRMTNSNVPRNSCCLLPGADFLSSKFWRKTQGQYLSPQFSRRGGAPSLATMHQTAGRNAETNFTSQRFRLDGGFITNPLAEVHTNSRNFA*