AlfalfaGEDB | Msa1299090

M. sativa cultivar zhongmu-4 / Msa1299090

KEGG: K09391
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006355 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0019219 GO:0019222 GO:0022402 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032875 GO:0032876 GO:0034641 GO:0034645 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045786 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051252 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090329 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901576 GO:1903506 GO:2000104 GO:2000112 GO:2000113 GO:2001141
PFAM: PF02319.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299090	XP_024636098.1	98.352	364	5	1	1	363	1	364	0.0	738

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299090	sp\|Q8LSZ4\|E2FE_ARATH	53.297	364	134	12	12	361	29	370	3.39e-106	320

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299090	G7JNS5	98.352	364	5	1	1	363	1	364	0.0	738

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TFs/TRs:

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Gene ID	Type	Classification
Msa1299090	TF	E2F-DP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0184700	Msa1299090	0.800595	1.338701e-48	-8.615850e-47
Msa1210960	Msa1299090	0.917581	4.458858e-86	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299090	MtrunA17_Chr4g0059551	96.143	363	7	2	1	362	1	357	0.0	714
Msa1299090	MtrunA17_Chr4g0025881	46.269	67	36	0	17	83	138	204	3.88e-12	67.4
Msa1299090	MtrunA17_Chr3g0142581	46.377	69	37	0	14	82	99	167	1.13e-11	65.5
Msa1299090	MtrunA17_Chr1g0177351	44.776	67	37	0	17	83	142	208	2.63e-11	64.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1299090	AT3G48160.1	53.825	366	131	13	12	362	29	371	5.91e-109	324
Msa1299090	AT3G48160.2	53.297	364	134	12	12	361	29	370	3.45e-107	320
Msa1299090	AT3G01330.1	46.705	349	151	6	13	361	17	330	4.32e-92	280
Msa1299090	AT5G14960.1	40.503	358	171	9	13	360	9	334	5.03e-77	241
Msa1299090	AT5G14960.2	35.945	217	116	6	154	360	38	241	9.31e-32	120
Msa1299090	AT5G22220.2	44.776	67	37	0	17	83	129	195	4.60e-11	64.3
Msa1299090	AT5G22220.3	44.776	67	37	0	17	83	129	195	4.83e-11	64.3

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Find 121 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGAAGACATTGAATG+AGG	0.592429	8_3:+60076371	Msa1299090:CDS
AAAATCCGTACATTCTATGT+TGG	0.531566	8_3:-60077124	None:intergenic
AAACCAGCATTCAGGTGGCT+AGG	0.559723	8_3:+60076345	Msa1299090:CDS
AAAGACGATGTTCGTTTAAT+TGG	0.211016	8_3:+60074044	Msa1299090:CDS
AAATGATAGTGGGCTATGTC+TGG	0.446224	8_3:+60076527	Msa1299090:CDS
AACAAAACGATTCTAGGAAA+AGG	0.426966	8_3:+60076412	Msa1299090:CDS
AACGTGCAACCTTATATTGA+AGG	0.458845	8_3:-60077275	None:intergenic
AAGAATCAGTATACCTGGAA+AGG	0.546799	8_3:+60074366	Msa1299090:CDS
AAGACAATGGAAAATGATAG+TGG	0.463413	8_3:+60076516	Msa1299090:CDS
AAGAGGGTGTTTGTGTGTGG+CGG	0.559507	8_3:-60073694	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATAAGAGAATTCAAATACG+AGG	+	chr8_3:60073474-60073493	Msa1299090:five_prime_UTR	30.0%
TTCATGGATAGATAGATAGA+TGG	-	chr8_3:60073516-60073535	None:intergenic	30.0%
AGAGCCGTTTAGATATAAAA+GGG	-	chr8_3:60073727-60073746	None:intergenic	30.0%
GAGAGATGTTTGATGAATTA+AGG	-	chr8_3:60073800-60073819	None:intergenic	30.0%
AATTTCGAATTTCAGATCCA+TGG	+	chr8_3:60073833-60073852	Msa1299090:exon	30.0%
AAAGACGATGTTCGTTTAAT+TGG	+	chr8_3:60074044-60074063	Msa1299090:CDS	30.0%
AACAGTTGTTAATGATGTTC+AGG	+	chr8_3:60074163-60074182	Msa1299090:intron	30.0%
ACATGAGGGAACAAAAATTA+AGG	-	chr8_3:60074265-60074284	None:intergenic	30.0%
AATCTCAATTCTTCACAAGA+AGG	+	chr8_3:60074550-60074569	Msa1299090:CDS	30.0%
TACTTTCGTGATTTAGGTAT+CGG	+	chr8_3:60074664-60074683	Msa1299090:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	60073464	60077343	60073464	ID=Msa1299090;Name=Msa1299090
chr8_3	mRNA	60073464	60077343	60073464	ID=Msa1299090-mRNA-1;Parent=Msa1299090;Name=Msa1299090-mRNA-1;_AED=0.13;_eAED=0.13;_QI=388\|1\|1\|1\|0.9\|0.90\|11\|420\|363
chr8_3	exon	60073464	60073934	60073464	ID=Msa1299090-mRNA-1:exon:17432;Parent=Msa1299090-mRNA-1
chr8_3	exon	60074025	60074092	60074025	ID=Msa1299090-mRNA-1:exon:17433;Parent=Msa1299090-mRNA-1
chr8_3	exon	60074185	60074237	60074185	ID=Msa1299090-mRNA-1:exon:17434;Parent=Msa1299090-mRNA-1
chr8_3	exon	60074348	60074425	60074348	ID=Msa1299090-mRNA-1:exon:17435;Parent=Msa1299090-mRNA-1
chr8_3	exon	60074532	60074591	60074532	ID=Msa1299090-mRNA-1:exon:17436;Parent=Msa1299090-mRNA-1
chr8_3	exon	60074680	60074815	60074680	ID=Msa1299090-mRNA-1:exon:17437;Parent=Msa1299090-mRNA-1
chr8_3	exon	60075776	60075840	60075776	ID=Msa1299090-mRNA-1:exon:17438;Parent=Msa1299090-mRNA-1
chr8_3	exon	60075954	60076026	60075954	ID=Msa1299090-mRNA-1:exon:17439;Parent=Msa1299090-mRNA-1
chr8_3	exon	60076105	60076184	60076105	ID=Msa1299090-mRNA-1:exon:17440;Parent=Msa1299090-mRNA-1
chr8_3	exon	60076324	60076740	60076324	ID=Msa1299090-mRNA-1:exon:17441;Parent=Msa1299090-mRNA-1
chr8_3	exon	60076945	60077343	60076945	ID=Msa1299090-mRNA-1:exon:17442;Parent=Msa1299090-mRNA-1
chr8_3	five_prime_UTR	60073464	60073851	60073464	ID=Msa1299090-mRNA-1:five_prime_utr;Parent=Msa1299090-mRNA-1
chr8_3	CDS	60073852	60073934	60073852	ID=Msa1299090-mRNA-1:cds;Parent=Msa1299090-mRNA-1
chr8_3	CDS	60074025	60074092	60074025	ID=Msa1299090-mRNA-1:cds;Parent=Msa1299090-mRNA-1
chr8_3	CDS	60074185	60074237	60074185	ID=Msa1299090-mRNA-1:cds;Parent=Msa1299090-mRNA-1
chr8_3	CDS	60074348	60074425	60074348	ID=Msa1299090-mRNA-1:cds;Parent=Msa1299090-mRNA-1
chr8_3	CDS	60074532	60074591	60074532	ID=Msa1299090-mRNA-1:cds;Parent=Msa1299090-mRNA-1
chr8_3	CDS	60074680	60074815	60074680	ID=Msa1299090-mRNA-1:cds;Parent=Msa1299090-mRNA-1
chr8_3	CDS	60075776	60075840	60075776	ID=Msa1299090-mRNA-1:cds;Parent=Msa1299090-mRNA-1
chr8_3	CDS	60075954	60076026	60075954	ID=Msa1299090-mRNA-1:cds;Parent=Msa1299090-mRNA-1
chr8_3	CDS	60076105	60076184	60076105	ID=Msa1299090-mRNA-1:cds;Parent=Msa1299090-mRNA-1
chr8_3	CDS	60076324	60076719	60076324	ID=Msa1299090-mRNA-1:cds;Parent=Msa1299090-mRNA-1
chr8_3	three_prime_UTR	60076720	60076740	60076720	ID=Msa1299090-mRNA-1:three_prime_utr;Parent=Msa1299090-mRNA-1
chr8_3	three_prime_UTR	60076945	60077343	60076945	ID=Msa1299090-mRNA-1:three_prime_utr;Parent=Msa1299090-mRNA-1

Gene Sequence

>Msa1299090

ATGGCTGCTTCTTCCTCCGATCCTCCTTCACGACACCACACTTACGATCGTAAACAAAAATCCCTCGGCCTCTTATGCACCAATTTCTTGAGCTTGTATAACAAAGACGATGTTCGTTTAATTGGTCTCGACGATGCTGCCGCCAAATTAGGTGTTGAGAGAAGACGGATCTATGATATTGTCAATGTTCTCGAAAGCATCGGGGTTCTTGCAAGAAAAGCCAAGAATCAGTATACCTGGAAAGGTTTTGCGGCAATTCCTGTTGCTTTACAGGAGCTTAAGGAAGAGGGTTTATGGCAGAATCTCAATTCTTCACAAGAAGGTGGCAATGAAGATGTGAAGGTATCGGATGAAGAGGATGAGGACGAATTGTTATCCCAAACCACTGGAAGTCAGGGTGAATCACTATCCCAACCCACTGGAAGTCAGAATGACAATCTAAACCCTAATTCAACTTTTCCCAGATCTTTGAAAAATGACAGAAGGGAAAAATCTCTGGCGCTGCTTACTCAGAATTTTGTCAAGCTCTTTGTCTGTTCTAACCTGGAAATGATATCCCTTGATGATGCAGCAAGGTTGTTGCTTGGAGATGCATATAATTCATCAACAATGAGAATCCCATCTGGCCTTGCAGCAAAAGTTAGGCGCCTTTATGATATTGCAAATGTGCTAACCTCCATGAACCTTATCGAGAAGACCCATACCACAGATACAAGAAAACCAGCATTCAGGTGGCTAGGCTTAAAAGGGAAGACATTGAATGAGGCATCACTTTACAATTCAAAACAAAACGATTCTAGGAAAAGGGCGTTTGGAAATGATGTCACAAACATAAGCTTTGCCAGGAATAGAACAGACTTGTTCATGGGTGGTGACTTTAAGAAGCAAAAGACAATGGAAAATGATAGTGGGCTATGTCTGGAAGATGTGAAACAAGGCATAAAACAGACTTCAGCTAACTATCAATTTGGTCCTTTTGCTCCTGCCTTTGTATCCAAAGCTGGAAGCTCTGAGAATAAAGTGAAGCAGGTGCATGACTGGGAGAGTCTCGCTACTGAACATTGCCCTCAGTATCAAAACCAAGGCAATTAA

Protein sequence

>Msa1299090

MAASSSDPPSRHHTYDRKQKSLGLLCTNFLSLYNKDDVRLIGLDDAAAKLGVERRRIYDIVNVLESIGVLARKAKNQYTWKGFAAIPVALQELKEEGLWQNLNSSQEGGNEDVKVSDEEDEDELLSQTTGSQGESLSQPTGSQNDNLNPNSTFPRSLKNDRREKSLALLTQNFVKLFVCSNLEMISLDDAARLLLGDAYNSSTMRIPSGLAAKVRRLYDIANVLTSMNLIEKTHTTDTRKPAFRWLGLKGKTLNEASLYNSKQNDSRKRAFGNDVTNISFARNRTDLFMGGDFKKQKTMENDSGLCLEDVKQGIKQTSANYQFGPFAPAFVSKAGSSENKVKQVHDWESLATEHCPQYQNQGN*