AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1299250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1299250 MtrunA17_Chr4g0059791 96.774 310 4 3 1 307 1 307 0.0 606
Msa1299250 MtrunA17_Chr4g0063491 65.934 91 30 1 216 306 154 243 2.68e-39 138
Msa1299250 MtrunA17_Chr8g0391451 62.626 99 34 2 211 307 49 146 2.24e-37 130
Msa1299250 MtrunA17_Chr4g0067531 52.439 82 36 2 220 301 51 129 7.00e-21 86.7
Msa1299250 MtrunA17_Chr2g0331651 46.067 89 46 2 213 300 2 89 3.12e-20 83.6
Msa1299250 MtrunA17_Chr8g0360861 50.633 79 38 1 222 300 12 89 5.18e-18 78.2
Msa1299250 MtrunA17_Chr6g0485891 44.444 81 44 1 222 302 46 125 7.17e-16 72.8
Msa1299250 MtrunA17_Chr1g0160981 45.946 74 38 1 214 285 51 124 1.13e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1299250 AT5G20110.1 67.778 90 28 1 217 306 118 206 2.09e-39 137
Msa1299250 AT1G23220.1 53.763 93 41 2 216 307 36 127 3.11e-27 103
Msa1299250 AT1G52245.1 46.154 91 47 2 213 302 2 91 7.52e-19 80.5
Msa1299250 AT4G15930.1 47.727 88 41 3 216 302 40 123 8.70e-19 80.9
Msa1299250 AT3G16120.2 47.126 87 45 1 216 302 6 91 1.08e-18 79.7
Msa1299250 AT3G16120.1 47.126 87 45 1 216 302 6 91 1.08e-18 79.7
Msa1299250 AT4G27360.1 46.591 88 46 1 216 303 6 92 4.08e-16 73.2

Find 111 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGTGGTTTCTCTTTA+AGG 0.077615 8_3:+60341398 None:intergenic
CAAATTTGATTTCAGTTAAA+TGG 0.124006 8_3:+60339837 None:intergenic
GGTGATTTCTTTGCAATTAA+TGG 0.142399 8_3:-60341172 Msa1299250:CDS
TTGAAGGAAGGTGGTGAATT+TGG 0.212017 8_3:-60340231 Msa1299250:exon
ATCAAATCTTGGTTTGGTTT+TGG 0.235547 8_3:+60341658 None:intergenic
TGTATTGTGGGGACTAGTTT+TGG 0.238004 8_3:-60340350 Msa1299250:CDS
GTACAGCAGTCTTAAATATA+AGG 0.257882 8_3:+60340264 None:intergenic
GGAGAGAGTTTCTGTTTCTA+TGG 0.258173 8_3:-60341245 Msa1299250:CDS
TTGAGGTGGTTTCTCTTTAA+GGG 0.259606 8_3:+60341399 None:intergenic
TGTGGGTCAATGATTGATTT+AGG 0.289700 8_3:-60341193 Msa1299250:CDS
GAGGGATAATGCTAGGGTTT+TGG 0.302500 8_3:+60341054 None:intergenic
ACACAAGTTGAGTGTTGTTT+TGG 0.323811 8_3:+60342051 None:intergenic
ATTGGAAAAGTGGAAACTTC+AGG 0.346164 8_3:-60341362 Msa1299250:CDS
ATGGTTAAAATATATTTGTT+TGG 0.352573 8_3:-60339705 Msa1299250:three_prime_UTR
TTTGTCATAGAAAGGTAAAA+AGG 0.362261 8_3:-60340171 Msa1299250:three_prime_UTR
TGTTTGGTGGGTGTTGTTGT+TGG 0.366216 8_3:+60341483 None:intergenic
CAAACGTGAGTCAACATGTT+TGG 0.366632 8_3:+60341552 None:intergenic
TTAGTGCATGTTTAATTTGA+CGG 0.394063 8_3:+60339974 None:intergenic
GTGGAGAAAGATAGAAGAAA+AGG 0.396037 8_3:+60341892 None:intergenic
GTTTGGTGGGTGTTGTTGTT+GGG 0.396439 8_3:+60341484 None:intergenic
GAAATATTGCAGGAATTTGA+TGG 0.396694 8_3:-60340395 Msa1299250:intron
TAGAAAGTGGTAAAGTGTAT+TGG 0.402477 8_3:+60341925 None:intergenic
CGCCAAGGGGTGTGTGTGTT+GGG 0.404458 8_3:+60342084 None:intergenic
AGAAAGTGGTAAAGTGTATT+GGG 0.405132 8_3:+60341926 None:intergenic
ACAGAAGATGAAACGAATTT+CGG 0.408803 8_3:-60340200 Msa1299250:three_prime_UTR
AAAAGAAGGCGGTGATGTTA+AGG 0.409463 8_3:-60341266 Msa1299250:CDS
AAAGGTGGTTGAGGATGTTA+AGG 0.409778 8_3:-60341320 Msa1299250:CDS
TTAGCTTATATAATTTCATA+TGG 0.416274 8_3:-60339724 Msa1299250:three_prime_UTR
ATCATGATAAGACAAACAAA+GGG 0.417190 8_3:+60340094 None:intergenic
GATTGTACAATAATTAGAAC+TGG 0.418704 8_3:-60339815 Msa1299250:three_prime_UTR
CAGGAATTTGATGGGGTGTA+TGG 0.421928 8_3:-60340386 Msa1299250:CDS
GGTATAGATTTGTCATAGAA+AGG 0.426753 8_3:-60340179 Msa1299250:three_prime_UTR
GCTTGAATCAAATCTTGGTT+TGG 0.433215 8_3:+60341652 None:intergenic
TAGTAGCTTGAATCAAATCT+TGG 0.435025 8_3:+60341647 None:intergenic
TATGAAGGCTATGTAACTAA+TGG 0.444710 8_3:-60339934 Msa1299250:three_prime_UTR
GTTGTTGAAGAATGAGAAAA+AGG 0.446134 8_3:-60341338 Msa1299250:CDS
TTTGGTTTGAGAATGTTGTT+TGG 0.446853 8_3:+60341570 None:intergenic
CCGGCGAAACACGGCACCAC+CGG 0.446993 8_3:-60341773 Msa1299250:CDS
TTGGAAAAGTGGAAACTTCA+GGG 0.459465 8_3:-60341361 Msa1299250:CDS
TTTGGTTGCTTAGCTAGAGC+AGG 0.461293 8_3:+60341736 None:intergenic
GCGCCAAGGGGTGTGTGTGT+TGG 0.469937 8_3:+60342083 None:intergenic
AGGATTTCTATATTTCTCAA+TGG 0.472685 8_3:-60340303 Msa1299250:CDS
ACATTGCTGTATTGTAGCAT+TGG 0.475963 8_3:-60340021 Msa1299250:three_prime_UTR
CCTTCTTGAGGGATAATGCT+AGG 0.479039 8_3:+60341047 None:intergenic
TTTGTGACCCATTCTGTTGG+AGG 0.480594 8_3:-60340323 Msa1299250:CDS
TGGTGCCGTGTTTCGCCGGC+GGG 0.480858 8_3:+60341777 None:intergenic
ACAGCATGGATCTGCATAAA+CGG 0.483580 8_3:+60341121 None:intergenic
TATGAATTATACCTTCTTGA+GGG 0.489208 8_3:+60341036 None:intergenic
TCATTTGTGACCCATTCTGT+TGG 0.494391 8_3:-60340326 Msa1299250:CDS
CTTCTTGAGGGATAATGCTA+GGG 0.494928 8_3:+60341048 None:intergenic
CCGGTGGTGCCGTGTTTCGC+CGG 0.500458 8_3:+60341773 None:intergenic
AGACTTACTTGAAGATATGA+AGG 0.504395 8_3:-60339949 Msa1299250:three_prime_UTR
TAAACTGCAACATCTGTGAC+TGG 0.504532 8_3:+60339781 None:intergenic
CCAGCATGGCACTGTATTGT+GGG 0.504817 8_3:-60340362 Msa1299250:CDS
TAGAAATCCTCCAACAGAAT+GGG 0.505407 8_3:+60340316 None:intergenic
GAGAGAGTTTCTGTTTCTAT+GGG 0.506756 8_3:-60341244 Msa1299250:CDS
GGGATATGACACATGAATGT+AGG 0.509486 8_3:+60342104 None:intergenic
CCTAAGTTAAGACTTTGAAA+AGG 0.512023 8_3:+60341604 None:intergenic
CTAGTTCAGAATTGGAAAAG+TGG 0.512540 8_3:-60341372 Msa1299250:CDS
CATGTTGACTCACGTTTGCA+AGG 0.516215 8_3:-60341547 Msa1299250:CDS
TAGAAACATCCTTTGTACAT+TGG 0.518235 8_3:-60340141 Msa1299250:three_prime_UTR
TGGAGAAAGATAGAAGAAAA+GGG 0.519020 8_3:+60341893 None:intergenic
TGTTTCGCCGGCGGGAAGAA+CGG 0.519846 8_3:+60341785 None:intergenic
AAATATTGCAGGAATTTGAT+GGG 0.521597 8_3:-60340394 Msa1299250:intron
ATGATTCTAACCATGCACTT+AGG 0.523566 8_3:+60340062 None:intergenic
GGTAGAAGAAAATCGTTATG+TGG 0.525728 8_3:-60341211 Msa1299250:CDS
ATCCCAACACACACACCCCT+TGG 0.528878 8_3:-60342086 Msa1299250:five_prime_UTR
GGGGAAAGAACATGGAGAAG+AGG 0.532950 8_3:+60341970 None:intergenic
CCCACAATACAGTGCCATGC+TGG 0.538117 8_3:+60340362 None:intergenic
TGCTTAGCTAGAGCAGGAGG+TGG 0.538974 8_3:+60341742 None:intergenic
AATCATGATAAGACAAACAA+AGG 0.539853 8_3:+60340093 None:intergenic
ATATGAATTATACCTTCTTG+AGG 0.545883 8_3:+60341035 None:intergenic
GGATATGACACATGAATGTA+GGG 0.546914 8_3:+60342105 None:intergenic
GTTTCTATGGGTCAAAGTGG+TGG 0.547379 8_3:-60341232 Msa1299250:CDS
ATAGAAATCCTCCAACAGAA+TGG 0.547634 8_3:+60340315 None:intergenic
AGGGTTATAGAAGTAGAAAG+TGG 0.550364 8_3:+60341912 None:intergenic
AAATATAGCTGAGGTTGTCA+TGG 0.552151 8_3:+60341519 None:intergenic
GCAGGAGGTGGATTTGTCTC+CGG 0.556134 8_3:+60341754 None:intergenic
TTCTTCCCGCCGGCGAAACA+CGG 0.557264 8_3:-60341782 Msa1299250:CDS
GTGGTGCCGTGTTTCGCCGG+CGG 0.566812 8_3:+60341776 None:intergenic
GCAACAATTGTGACCACCGT+TGG 0.572684 8_3:+60339885 None:intergenic
GTGTTGTTGGGGAAAGAACA+TGG 0.574339 8_3:+60341962 None:intergenic
TTCTAGCACAATCAACAGCA+TGG 0.575374 8_3:+60341107 None:intergenic
AAAGAACATGGAGAAGAGGA+AGG 0.575464 8_3:+60341974 None:intergenic
ACCAGCATGGCACTGTATTG+TGG 0.577524 8_3:-60340363 Msa1299250:CDS
CCTAGCATTATCCCTCAAGA+AGG 0.579726 8_3:-60341047 Msa1299250:intron
TGAAGGTGATGATGAGAAGA+TGG 0.583761 8_3:+60341693 None:intergenic
GGTTGCTTAGCTAGAGCAGG+AGG 0.583906 8_3:+60341739 None:intergenic
GTAGAAGAAAATCGTTATGT+GGG 0.594942 8_3:-60341210 Msa1299250:CDS
CACACGTAAGTGCTAGTCAA+AGG 0.609844 8_3:-60342136 Msa1299250:five_prime_UTR
TCTGTTTCTATGGGTCAAAG+TGG 0.611585 8_3:-60341235 Msa1299250:CDS
TGATGAATCAAATATAGCTG+AGG 0.614815 8_3:+60341510 None:intergenic
GGAGGTGGATTTGTCTCCGG+TGG 0.623941 8_3:+60341757 None:intergenic
AAACTGCAACATCTGTGACT+GGG 0.626950 8_3:+60339782 None:intergenic
AAAAGCAGATTGAAGGAAGG+TGG 0.627500 8_3:-60340240 Msa1299250:exon
TGGCACACCGTTCTTCCCGC+CGG 0.632791 8_3:-60341792 Msa1299250:CDS
GTAATCAACATTATATCCAA+CGG 0.635891 8_3:-60339901 Msa1299250:three_prime_UTR
CAGCATGGCACTGTATTGTG+GGG 0.649093 8_3:-60340361 Msa1299250:CDS
AAGCATAAGCCAATGTACAA+AGG 0.654076 8_3:+60340132 None:intergenic
TGGAAAAGTGGAAACTTCAG+GGG 0.656471 8_3:-60341360 Msa1299250:CDS
TTTGGATAGTGAGAAGGTGA+AGG 0.666862 8_3:+60341676 None:intergenic
GGTGTGCCATGTTGAATAGA+CGG 0.676331 8_3:+60341806 None:intergenic
TCATTAGATTGAGACTTGTG+CGG 0.683098 8_3:+60342016 None:intergenic
AGGTGATGATGAGAAGATGG+TGG 0.684245 8_3:+60341696 None:intergenic
TTTGCACCGTCTATTCAACA+TGG 0.687135 8_3:-60341812 Msa1299250:exon
AATATTGCAGGAATTTGATG+GGG 0.687792 8_3:-60340393 Msa1299250:intron
TCATGATAAGACAAACAAAG+GGG 0.706871 8_3:+60340095 None:intergenic
ATCAACATTATATCCAACGG+TGG 0.711161 8_3:-60339898 Msa1299250:three_prime_UTR
CATGATAAGACAAACAAAGG+GGG 0.713361 8_3:+60340096 None:intergenic
GCATGGATCTGCATAAACGG+TGG 0.717762 8_3:+60341124 None:intergenic
ATGGGGTGTATGGACCAGCA+TGG 0.734479 8_3:-60340376 Msa1299250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGATATTTTTTGGTTTTT+GGG + chr8_3:60339981-60340000 None:intergenic 15.0%
!!! TGTTTTAGTATGATATTTTT+TGG + chr8_3:60339989-60340008 None:intergenic 15.0%
!! TTAGCTTATATAATTTCATA+TGG - chr8_3:60342108-60342127 Msa1299250:five_prime_UTR 15.0%
!! ATGGTTAAAATATATTTGTT+TGG - chr8_3:60342127-60342146 Msa1299250:five_prime_UTR 15.0%
!!! GTATGATATTTTTTGGTTTT+TGG + chr8_3:60339982-60340001 None:intergenic 20.0%
!!! AGTTTTGAATGAATTGATTA+TGG - chr8_3:60340915-60340934 Msa1299250:intron 20.0%
!!! TCAAATCATTTTGCTAAATT+TGG + chr8_3:60341238-60341257 None:intergenic 20.0%
!!! TTATAATGATTTTCTTTAGG+AGG + chr8_3:60341309-60341328 None:intergenic 20.0%
!!! CGTTTATAATGATTTTCTTT+AGG + chr8_3:60341312-60341331 None:intergenic 20.0%
!! AAAGAAAATCATTATAAACG+TGG - chr8_3:60341312-60341331 Msa1299250:CDS 20.0%
!!! TTTTGCATTAGGAATAATTT+AGG - chr8_3:60341340-60341359 Msa1299250:CDS 20.0%
!! TTTGCATTAGGAATAATTTA+GGG - chr8_3:60341341-60341360 Msa1299250:CDS 20.0%
!!! TGAATATTTTAGTGAATGAT+TGG - chr8_3:60341390-60341409 Msa1299250:CDS 20.0%
!!! CAAATTTGATTTCAGTTAAA+TGG + chr8_3:60341998-60342017 None:intergenic 20.0%
!! TTAGTACTGTGATTTTTGTT+TGG + chr8_3:60340368-60340387 None:intergenic 25.0%
!!! ACACTATCTTTTTCATGATT+TGG + chr8_3:60340393-60340412 None:intergenic 25.0%
!!! TAGAAAAGCTTTTGATAGTA+TGG - chr8_3:60340743-60340762 Msa1299250:intron 25.0%
! TATGAATTATACCTTCTTGA+GGG + chr8_3:60340799-60340818 None:intergenic 25.0%
! ATATGAATTATACCTTCTTG+AGG + chr8_3:60340800-60340819 None:intergenic 25.0%
!!! ACTTACATTCAAGTCATTTT+GGG + chr8_3:60341152-60341171 None:intergenic 25.0%
!! TCATTTTGCTAAATTTGGAT+CGG + chr8_3:60341233-60341252 None:intergenic 25.0%
! AAATATTGCAGGAATTTGAT+GGG - chr8_3:60341438-60341457 Msa1299250:CDS 25.0%
! AGGATTTCTATATTTCTCAA+TGG - chr8_3:60341529-60341548 Msa1299250:CDS 25.0%
! TTTGTCATAGAAAGGTAAAA+AGG - chr8_3:60341661-60341680 Msa1299250:CDS 25.0%
! ATCATGATAAGACAAACAAA+GGG + chr8_3:60341741-60341760 None:intergenic 25.0%
! AATCATGATAAGACAAACAA+AGG + chr8_3:60341742-60341761 None:intergenic 25.0%
!!! TTAGTGCATGTTTAATTTGA+CGG + chr8_3:60341861-60341880 None:intergenic 25.0%
! GTAATCAACATTATATCCAA+CGG - chr8_3:60341931-60341950 Msa1299250:five_prime_UTR 25.0%
! GATTGTACAATAATTAGAAC+TGG - chr8_3:60342017-60342036 Msa1299250:five_prime_UTR 25.0%
AGAAAGTGGTAAAGTGTATT+GGG + chr8_3:60339909-60339928 None:intergenic 30.0%
TAGAAAGTGGTAAAGTGTAT+TGG + chr8_3:60339910-60339929 None:intergenic 30.0%
TGGAGAAAGATAGAAGAAAA+GGG + chr8_3:60339942-60339961 None:intergenic 30.0%
!!! GATATTTTTTGGTTTTTGGG+TGG + chr8_3:60339978-60339997 None:intergenic 30.0%
!!! ATCAAATCTTGGTTTGGTTT+TGG + chr8_3:60340177-60340196 None:intergenic 30.0%
TAGTAGCTTGAATCAAATCT+TGG + chr8_3:60340188-60340207 None:intergenic 30.0%
! CCTAAGTTAAGACTTTGAAA+AGG + chr8_3:60340231-60340250 None:intergenic 30.0%
! CCTTTTCAAAGTCTTAACTT+AGG - chr8_3:60340228-60340247 Msa1299250:three_prime_UTR 30.0%
!! TTTGGTTTGAGAATGTTGTT+TGG + chr8_3:60340265-60340284 None:intergenic 30.0%
TGATGAATCAAATATAGCTG+AGG + chr8_3:60340325-60340344 None:intergenic 30.0%
!! TACTGTGATTTTTGTTTGGT+GGG + chr8_3:60340364-60340383 None:intergenic 30.0%
!!! GTGTTTTTTTGCATTCTAAG+AGG - chr8_3:60340409-60340428 Msa1299250:intron 30.0%
! AATTCTGAACTAGGTTTTTG+AGG + chr8_3:60340453-60340472 None:intergenic 30.0%
TCAAAAACCTAGTTCAGAAT+TGG - chr8_3:60340452-60340471 Msa1299250:intron 30.0%
GTTGTTGAAGAATGAGAAAA+AGG - chr8_3:60340494-60340513 Msa1299250:intron 30.0%
GTCAAAAAAAGTAACTGTTG+TGG - chr8_3:60340542-60340561 Msa1299250:intron 30.0%
GTAGAAGAAAATCGTTATGT+GGG - chr8_3:60340622-60340641 Msa1299250:intron 30.0%
GGTGATTTCTTTGCAATTAA+TGG - chr8_3:60340660-60340679 Msa1299250:intron 30.0%
TGCAATTAATGGAGCAAAAA+TGG - chr8_3:60340671-60340690 Msa1299250:intron 30.0%
! GTTAGACTTGACACTTTTAT+CGG - chr8_3:60340993-60341012 Msa1299250:intron 30.0%
! TTAGACTTGACACTTTTATC+GGG - chr8_3:60340994-60341013 Msa1299250:intron 30.0%
! CACTTACATTCAAGTCATTT+TGG + chr8_3:60341153-60341172 None:intergenic 30.0%
GTGTATGTTTGAATTTAGTG+TGG - chr8_3:60341170-60341189 Msa1299250:CDS 30.0%
! ATTTTGCTAAATTTGGATCG+GGG + chr8_3:60341231-60341250 None:intergenic 30.0%
! CATTTTGCTAAATTTGGATC+GGG + chr8_3:60341232-60341251 None:intergenic 30.0%
! GAATGATTGGTCAATGAATT+CGG - chr8_3:60341403-60341422 Msa1299250:CDS 30.0%
GAAATATTGCAGGAATTTGA+TGG - chr8_3:60341437-60341456 Msa1299250:CDS 30.0%
AATATTGCAGGAATTTGATG+GGG - chr8_3:60341439-60341458 Msa1299250:CDS 30.0%
GTACAGCAGTCTTAAATATA+AGG + chr8_3:60341571-60341590 None:intergenic 30.0%
ACAGAAGATGAAACGAATTT+CGG - chr8_3:60341632-60341651 Msa1299250:CDS 30.0%
GGTATAGATTTGTCATAGAA+AGG - chr8_3:60341653-60341672 Msa1299250:CDS 30.0%
TAGAAACATCCTTTGTACAT+TGG - chr8_3:60341691-60341710 Msa1299250:CDS 30.0%
TCATGATAAGACAAACAAAG+GGG + chr8_3:60341740-60341759 None:intergenic 30.0%
AGACTTACTTGAAGATATGA+AGG - chr8_3:60341883-60341902 Msa1299250:five_prime_UTR 30.0%
TATGAAGGCTATGTAACTAA+TGG - chr8_3:60341898-60341917 Msa1299250:five_prime_UTR 30.0%
GGATATGACACATGAATGTA+GGG + chr8_3:60339730-60339749 None:intergenic 35.0%
!!! ACACAAGTTGAGTGTTGTTT+TGG + chr8_3:60339784-60339803 None:intergenic 35.0%
TCATTAGATTGAGACTTGTG+CGG + chr8_3:60339819-60339838 None:intergenic 35.0%
!!! TAGCTTTTCTAGGTGTTGTT+GGG + chr8_3:60339885-60339904 None:intergenic 35.0%
!! TTAGCTTTTCTAGGTGTTGT+TGG + chr8_3:60339886-60339905 None:intergenic 35.0%
!!! TGTATTGGGTTAGCTTTTCT+AGG + chr8_3:60339895-60339914 None:intergenic 35.0%
AGGGTTATAGAAGTAGAAAG+TGG + chr8_3:60339923-60339942 None:intergenic 35.0%
GTGGAGAAAGATAGAAGAAA+AGG + chr8_3:60339943-60339962 None:intergenic 35.0%
!!! TTTGGTTTTGGATAGTGAGA+AGG + chr8_3:60340165-60340184 None:intergenic 35.0%
GCTTGAATCAAATCTTGGTT+TGG + chr8_3:60340183-60340202 None:intergenic 35.0%
AAATATAGCTGAGGTTGTCA+TGG + chr8_3:60340316-60340335 None:intergenic 35.0%
!! GTACTGTGATTTTTGTTTGG+TGG + chr8_3:60340365-60340384 None:intergenic 35.0%
TTGAGGTGGTTTCTCTTTAA+GGG + chr8_3:60340436-60340455 None:intergenic 35.0%
! TTTGAGGTGGTTTCTCTTTA+AGG + chr8_3:60340437-60340456 None:intergenic 35.0%
! CACTTTTCCAATTCTGAACT+AGG + chr8_3:60340462-60340481 None:intergenic 35.0%
CTAGTTCAGAATTGGAAAAG+TGG - chr8_3:60340460-60340479 Msa1299250:intron 35.0%
ATTGGAAAAGTGGAAACTTC+AGG - chr8_3:60340470-60340489 Msa1299250:intron 35.0%
TTGGAAAAGTGGAAACTTCA+GGG - chr8_3:60340471-60340490 Msa1299250:intron 35.0%
GTTGAAGAATGAGAAAAAGG+TGG - chr8_3:60340497-60340516 Msa1299250:intron 35.0%
GTAACTGTTGTGGAAAAAGA+AGG - chr8_3:60340552-60340571 Msa1299250:intron 35.0%
!! GAGAGAGTTTCTGTTTCTAT+GGG - chr8_3:60340588-60340607 Msa1299250:intron 35.0%
GGTAGAAGAAAATCGTTATG+TGG - chr8_3:60340621-60340640 Msa1299250:intron 35.0%
! TGTGGGTCAATGATTGATTT+AGG - chr8_3:60340639-60340658 Msa1299250:intron 35.0%
CCTAGTTCAATTGACAATGT+CGG - chr8_3:60340966-60340985 Msa1299250:intron 35.0%
ATCTAAGGACCACTAAAACT+CGG + chr8_3:60341052-60341071 None:intergenic 35.0%
ACATACGCTAAAGTCATCTA+AGG + chr8_3:60341067-60341086 None:intergenic 35.0%
TATGTTGACGAGAGAATGAT+TGG - chr8_3:60341082-60341101 Msa1299250:CDS 35.0%
!! ACGTGGTTTACTTTTGCATT+AGG - chr8_3:60341329-60341348 Msa1299250:CDS 35.0%
TAGAAATCCTCCAACAGAAT+GGG + chr8_3:60341519-60341538 None:intergenic 35.0%
ATAGAAATCCTCCAACAGAA+TGG + chr8_3:60341520-60341539 None:intergenic 35.0%
CTGTACAAAAAGCAGATTGA+AGG - chr8_3:60341585-60341604 Msa1299250:CDS 35.0%
ACAAAAAGCAGATTGAAGGA+AGG - chr8_3:60341589-60341608 Msa1299250:CDS 35.0%
AAGCATAAGCCAATGTACAA+AGG + chr8_3:60341703-60341722 None:intergenic 35.0%
CATGATAAGACAAACAAAGG+GGG + chr8_3:60341739-60341758 None:intergenic 35.0%
! TTTTACAGAACCTAAGTGCA+TGG - chr8_3:60341760-60341779 Msa1299250:CDS 35.0%
ATGATTCTAACCATGCACTT+AGG + chr8_3:60341773-60341792 None:intergenic 35.0%
ACATTGCTGTATTGTAGCAT+TGG - chr8_3:60341811-60341830 Msa1299250:exon 35.0%
ATCAACATTATATCCAACGG+TGG - chr8_3:60341934-60341953 Msa1299250:five_prime_UTR 35.0%
!! ACACGATCAATTTTTGAGCT+CGG - chr8_3:60342085-60342104 Msa1299250:five_prime_UTR 35.0%
GGGATATGACACATGAATGT+AGG + chr8_3:60339731-60339750 None:intergenic 40.0%
AAAGAACATGGAGAAGAGGA+AGG + chr8_3:60339861-60339880 None:intergenic 40.0%
!!! AGCTTTTCTAGGTGTTGTTG+GGG + chr8_3:60339884-60339903 None:intergenic 40.0%
!!! TGGGTGGTTTTGTTTTGTTG+TGG + chr8_3:60339962-60339981 None:intergenic 40.0%
TTTGCACCGTCTATTCAACA+TGG - chr8_3:60340020-60340039 Msa1299250:three_prime_UTR 40.0%
!!! GTTTTTGTAGTGGTGGGTTT+TGG + chr8_3:60340117-60340136 None:intergenic 40.0%
! ATGGTGGTTTTTGTAGTGGT+GGG + chr8_3:60340123-60340142 None:intergenic 40.0%
!! GAAGATGGTGGTTTTTGTAG+TGG + chr8_3:60340127-60340146 None:intergenic 40.0%
! TGAAGGTGATGATGAGAAGA+TGG + chr8_3:60340142-60340161 None:intergenic 40.0%
TTTGGATAGTGAGAAGGTGA+AGG + chr8_3:60340159-60340178 None:intergenic 40.0%
CAAACGTGAGTCAACATGTT+TGG + chr8_3:60340283-60340302 None:intergenic 40.0%
!! TCTGAACTAGGTTTTTGAGG+TGG + chr8_3:60340450-60340469 None:intergenic 40.0%
TGGAAAAGTGGAAACTTCAG+GGG - chr8_3:60340472-60340491 Msa1299250:intron 40.0%
GAATGAGAAAAAGGTGGTTG+AGG - chr8_3:60340503-60340522 Msa1299250:intron 40.0%
AAAGGTGGTTGAGGATGTTA+AGG - chr8_3:60340512-60340531 Msa1299250:intron 40.0%
ACTGTTGTGGAAAAAGAAGG+CGG - chr8_3:60340555-60340574 Msa1299250:intron 40.0%
AAAAGAAGGCGGTGATGTTA+AGG - chr8_3:60340566-60340585 Msa1299250:intron 40.0%
!! GGAGAGAGTTTCTGTTTCTA+TGG - chr8_3:60340587-60340606 Msa1299250:intron 40.0%
!! TCTGTTTCTATGGGTCAAAG+TGG - chr8_3:60340597-60340616 Msa1299250:intron 40.0%
ACAGCATGGATCTGCATAAA+CGG + chr8_3:60340714-60340733 None:intergenic 40.0%
TTCTAGCACAATCAACAGCA+TGG + chr8_3:60340728-60340747 None:intergenic 40.0%
CTTCTTGAGGGATAATGCTA+GGG + chr8_3:60340787-60340806 None:intergenic 40.0%
CCGACATTGTCAATTGAACT+AGG + chr8_3:60340969-60340988 None:intergenic 40.0%
! TTTTATCGGGTTCGAACCAA+GGG - chr8_3:60341007-60341026 Msa1299250:intron 40.0%
GTCAATGAATTCGGTTGTGT+GGG - chr8_3:60341412-60341431 Msa1299250:CDS 40.0%
! TGTGTGGGTTGAAATATTGC+AGG - chr8_3:60341427-60341446 Msa1299250:CDS 40.0%
!!! TGTATTGTGGGGACTAGTTT+TGG - chr8_3:60341482-60341501 Msa1299250:CDS 40.0%
TCATTTGTGACCCATTCTGT+TGG - chr8_3:60341506-60341525 Msa1299250:CDS 40.0%
AAAAGCAGATTGAAGGAAGG+TGG - chr8_3:60341592-60341611 Msa1299250:CDS 40.0%
TTGAAGGAAGGTGGTGAATT+TGG - chr8_3:60341601-60341620 Msa1299250:CDS 40.0%
! ACAAAGGGGGTGATTCATTT+TGG + chr8_3:60341726-60341745 None:intergenic 40.0%
AAACTGCAACATCTGTGACT+GGG + chr8_3:60342053-60342072 None:intergenic 40.0%
TAAACTGCAACATCTGTGAC+TGG + chr8_3:60342054-60342073 None:intergenic 40.0%
CACACGTAAGTGCTAGTCAA+AGG - chr8_3:60339696-60339715 Msa1299250:three_prime_UTR 45.0%
! GTGTTGTTGGGGAAAGAACA+TGG + chr8_3:60339873-60339892 None:intergenic 45.0%
GGTGTGCCATGTTGAATAGA+CGG + chr8_3:60340029-60340048 None:intergenic 45.0%
TTTGGTTGCTTAGCTAGAGC+AGG + chr8_3:60340099-60340118 None:intergenic 45.0%
!! GATGGTGGTTTTTGTAGTGG+TGG + chr8_3:60340124-60340143 None:intergenic 45.0%
! AGGTGATGATGAGAAGATGG+TGG + chr8_3:60340139-60340158 None:intergenic 45.0%
!! CATGTTGACTCACGTTTGCA+AGG - chr8_3:60340285-60340304 Msa1299250:CDS 45.0%
!! GTTTGGTGGGTGTTGTTGTT+GGG + chr8_3:60340351-60340370 None:intergenic 45.0%
!! TGTTTGGTGGGTGTTGTTGT+TGG + chr8_3:60340352-60340371 None:intergenic 45.0%
GTTTCTATGGGTCAAAGTGG+TGG - chr8_3:60340600-60340619 Msa1299250:intron 45.0%
! GAGGGATAATGCTAGGGTTT+TGG + chr8_3:60340781-60340800 None:intergenic 45.0%
CCTTCTTGAGGGATAATGCT+AGG + chr8_3:60340788-60340807 None:intergenic 45.0%
CCTAGCATTATCCCTCAAGA+AGG - chr8_3:60340785-60340804 Msa1299250:intron 45.0%
! CTTTTATCGGGTTCGAACCA+AGG - chr8_3:60341006-60341025 Msa1299250:intron 45.0%
!! TAGCTGTGTCCGAGTTTTAG+TGG - chr8_3:60341040-60341059 Msa1299250:intron 45.0%
GGTCAATGAATTCGGTTGTG+TGG - chr8_3:60341411-60341430 Msa1299250:CDS 45.0%
CAGGAATTTGATGGGGTGTA+TGG - chr8_3:60341446-60341465 Msa1299250:CDS 45.0%
TTTGTGACCCATTCTGTTGG+AGG - chr8_3:60341509-60341528 Msa1299250:CDS 45.0%
!!! TTGGTTTTGTCACGCGCCAA+GGG + chr8_3:60339765-60339784 None:intergenic 50.0%
!!! TTTGGTTTTGTCACGCGCCA+AGG + chr8_3:60339766-60339785 None:intergenic 50.0%
GGGGAAAGAACATGGAGAAG+AGG + chr8_3:60339865-60339884 None:intergenic 50.0%
GCATGGATCTGCATAAACGG+TGG + chr8_3:60340711-60340730 None:intergenic 50.0%
ACCAGCATGGCACTGTATTG+TGG - chr8_3:60341469-60341488 Msa1299250:CDS 50.0%
CCAGCATGGCACTGTATTGT+GGG - chr8_3:60341470-60341489 Msa1299250:CDS 50.0%
CAGCATGGCACTGTATTGTG+GGG - chr8_3:60341471-60341490 Msa1299250:CDS 50.0%
GCAACAATTGTGACCACCGT+TGG + chr8_3:60341950-60341969 None:intergenic 50.0%
ATCCCAACACACACACCCCT+TGG - chr8_3:60339746-60339765 Msa1299250:three_prime_UTR 55.0%
!!! TGGTTTTGTCACGCGCCAAG+GGG + chr8_3:60339764-60339783 None:intergenic 55.0%
! GCAGGAGGTGGATTTGTCTC+CGG + chr8_3:60340081-60340100 None:intergenic 55.0%
TGCTTAGCTAGAGCAGGAGG+TGG + chr8_3:60340093-60340112 None:intergenic 55.0%
GGTTGCTTAGCTAGAGCAGG+AGG + chr8_3:60340096-60340115 None:intergenic 55.0%
ATCGGGTTCGAACCAAGGGT+AGG - chr8_3:60341011-60341030 Msa1299250:intron 55.0%
TCGGGTTCGAACCAAGGGTA+GGG - chr8_3:60341012-60341031 Msa1299250:intron 55.0%
CAGCTACAAGACCCTACCCT+TGG + chr8_3:60341026-60341045 None:intergenic 55.0%
ATGGGGTGTATGGACCAGCA+TGG - chr8_3:60341456-60341475 Msa1299250:CDS 55.0%
CCCACAATACAGTGCCATGC+TGG + chr8_3:60341473-60341492 None:intergenic 55.0%
! CGCCAAGGGGTGTGTGTGTT+GGG + chr8_3:60339751-60339770 None:intergenic 60.0%
TGTTTCGCCGGCGGGAAGAA+CGG + chr8_3:60340050-60340069 None:intergenic 60.0%
TTCTTCCCGCCGGCGAAACA+CGG - chr8_3:60340050-60340069 Msa1299250:three_prime_UTR 60.0%
! GGAGGTGGATTTGTCTCCGG+TGG + chr8_3:60340078-60340097 None:intergenic 60.0%
! GCGCCAAGGGGTGTGTGTGT+TGG + chr8_3:60339752-60339771 None:intergenic 65.0%
TGGCACACCGTTCTTCCCGC+CGG - chr8_3:60340040-60340059 Msa1299250:three_prime_UTR 65.0%
!! TGGTGCCGTGTTTCGCCGGC+GGG + chr8_3:60340058-60340077 None:intergenic 70.0%
!! GTGGTGCCGTGTTTCGCCGG+CGG + chr8_3:60340059-60340078 None:intergenic 70.0%
!! CCGGTGGTGCCGTGTTTCGC+CGG + chr8_3:60340062-60340081 None:intergenic 70.0%
CCGGCGAAACACGGCACCAC+CGG - chr8_3:60340059-60340078 Msa1299250:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 60339692 60342162 60339692 ID=Msa1299250;Name=Msa1299250
chr8_3 mRNA 60339692 60342162 60339692 ID=Msa1299250-mRNA-1;Parent=Msa1299250;Name=Msa1299250-mRNA-1;_AED=0.03;_eAED=0.03;_QI=347|1|1|1|1|1|2|558|307
chr8_3 exon 60339692 60340405 60339692 ID=Msa1299250-mRNA-1:exon:17569;Parent=Msa1299250-mRNA-1
chr8_3 exon 60341048 60342162 60341048 ID=Msa1299250-mRNA-1:exon:17568;Parent=Msa1299250-mRNA-1
chr8_3 five_prime_UTR 60341816 60342162 60341816 ID=Msa1299250-mRNA-1:five_prime_utr;Parent=Msa1299250-mRNA-1
chr8_3 CDS 60341048 60341815 60341048 ID=Msa1299250-mRNA-1:cds;Parent=Msa1299250-mRNA-1
chr8_3 CDS 60340250 60340405 60340250 ID=Msa1299250-mRNA-1:cds;Parent=Msa1299250-mRNA-1
chr8_3 three_prime_UTR 60339692 60340249 60339692 ID=Msa1299250-mRNA-1:three_prime_utr;Parent=Msa1299250-mRNA-1
Gene Sequence

>Msa1299250

ATGGCACACCGTTCTTCCCGCCGGCGAAACACGGCACCACCGGAGACAAATCCACCTCCTGCTCTAGCTAAGCAACCAAAACCCACCACTACAAAAACCACCATCTTCTCATCATCACCTTCACCTTCTCACTATCCAAAACCAAACCAAGATTTGATTCAAGCTACTAAATCTTCTTTTTTAGTTAAACCTTTTCAAAGTCTTAACTTAGGTAGCAAAAAAACCAAACAACATTCTCAAACCAAACATGTTGACTCACGTTTGCAAGGTAAATCCATGACAACCTCAGCTATATTTGATTCATCAAAACCCAACAACAACACCCACCAAACAAAAATCACAGTACTAACACCAAATCATGAAAAAGATAGTGTTTTTTTGCATTCTAAGAGGACCCTTAAAGAGAAACCACCTCAAAAACCTAGTTCAGAATTGGAAAAGTGGAAACTTCAGGGGTTGTTGAAGAATGAGAAAAAGGTGGTTGAGGATGTTAAGGTTGAAAAGTCAAAAAAAGTAACTGTTGTGGAAAAAGAAGGCGGTGATGTTAAGGAGAGAGTTTCTGTTTCTATGGGTCAAAGTGGTGGTAGAAGAAAATCGTTATGTGGGTCAATGATTGATTTAGGTGATTTCTTTGCAATTAATGGAGCAAAAATGGTTTCAGCTGATATGCCACCGTTTATGCAGATCCATGCTGTTGATTGTGCTAGAAAAGCTTTTGATAGTATGGAGAAATTCACTTCCAAAACCCTAGCATTATCCCTCAAGAAGGAATTTGATGGGGTGTATGGACCAGCATGGCACTGTATTGTGGGGACTAGTTTTGGATCATTTGTGACCCATTCTGTTGGAGGATTTCTATATTTCTCAATGGATCAAAAATTGTACATCCTTATATTTAAGACTGCTGTACAAAAAGCAGATTGA
Protein sequence

>Msa1299250

MAHRSSRRRNTAPPETNPPPALAKQPKPTTTKTTIFSSSPSPSHYPKPNQDLIQATKSSFLVKPFQSLNLGSKKTKQHSQTKHVDSRLQGKSMTTSAIFDSSKPNNNTHQTKITVLTPNHEKDSVFLHSKRTLKEKPPQKPSSELEKWKLQGLLKNEKKVVEDVKVEKSKKVTVVEKEGGDVKERVSVSMGQSGGRRKSLCGSMIDLGDFFAINGAKMVSADMPPFMQIHAVDCARKAFDSMEKFTSKTLALSLKKEFDGVYGPAWHCIVGTSFGSFVTHSVGGFLYFSMDQKLYILIFKTAVQKAD*