AlfalfaGEDB | Msa1301030

M. sativa cultivar zhongmu-4 / Msa1301030

KEGG: K00083
GO: -
PFAM: PF08240.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301030	sp\|Q9ZRF1\|MTDH_FRAAN	77.809	356	79	0	6	361	4	359	0.0	555

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301030	G7JFC2	96.399	361	13	0	1	361	1	361	0.0	723

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0046680	Msa1301030	-0.813808	2.122062e-51	-8.615850e-47
Msa0466850	Msa1301030	-0.814679	1.362837e-51	-8.615850e-47
Msa0513670	Msa1301030	-0.812988	3.213439e-51	-8.615850e-47
Msa1261860	Msa1301030	0.996037	1.138603e-222	-8.615850e-47
Msa1301030	Msa1327400	0.800583	1.346379e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	bit score
Msa1301030	MtrunA17_Chr4g0064291	96.399	361	13	1	361	1	361	723
Msa1301030	MtrunA17_Chr5g0411821	83.380	361	60	1	361	1	361	620
Msa1301030	MtrunA17_Chr5g0411841	81.994	361	65	1	361	1	361	598
Msa1301030	MtrunA17_Chr5g0411901	82.203	354	63	7	360	8	361	595
Msa1301030	MtrunA17_Chr5g0411811	82.271	361	64	1	361	1	361	595
Msa1301030	MtrunA17_Chr5g0411881	81.356	354	66	7	360	8	361	590
Msa1301030	MtrunA17_Chr5g0411871	79.096	354	74	8	361	23	376	582
Msa1301030	MtrunA17_Chr5g0411931	80.055	361	72	1	361	1	361	581
Msa1301030	MtrunA17_Chr5g0411911	77.778	351	78	10	360	12	362	563
Msa1301030	MtrunA17_Chr5g0411801	75.346	361	89	1	361	33	393	556

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1301030	AT4G37980.1	70.402	348	103	0	13	360	9	356	1.26e-171	482
Msa1301030	AT4G37990.1	70.402	348	103	0	13	360	9	356	1.00e-167	472
Msa1301030	AT4G37970.1	62.535	355	133	0	6	360	7	361	1.79e-163	462
Msa1301030	AT4G39330.1	61.838	359	137	0	1	359	1	359	3.17e-163	461
Msa1301030	AT2G21730.1	61.473	353	134	2	11	361	5	357	6.29e-154	438
Msa1301030	AT2G21890.1	61.648	352	134	1	11	361	5	356	8.90e-154	437
Msa1301030	AT4G37980.2	70.175	285	85	0	13	297	9	293	1.56e-133	383
Msa1301030	AT4G39330.2	61.130	301	117	0	1	301	1	301	3.38e-130	375
Msa1301030	AT4G37970.4	60.616	292	115	0	6	297	7	298	1.40e-125	363
Msa1301030	AT1G72680.1	52.299	348	165	1	11	358	9	355	4.87e-117	343

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Find 113 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTAAACTTGGGTTCTC+CGG	0.430013	8_3:-62938223	Msa1301030:three_prime_UTR
AAAGCATACCCAGGAACTAG+TGG	0.598271	8_3:+62939777	None:intergenic
AAAGGAAGCAAGAGAACTCT+TGG	0.393117	8_3:-62939218	Msa1301030:CDS
AAAGTAGAAAAGTTTAAAGT+TGG	0.357138	8_3:-62939628	Msa1301030:CDS
AAATACAGACATGAGATTGT+TGG	0.489466	8_3:-62939673	Msa1301030:intron
AACCTTCTGGAGAAATTGAA+AGG	0.496825	8_3:+62940356	None:intergenic
AAGAGTTTCAAGAGGAAATC+GGG	0.481161	8_3:-62938308	Msa1301030:three_prime_UTR
AAGGAAGCAAGAGAACTCTT+GGG	0.547722	8_3:-62939217	Msa1301030:CDS
AAGGGGAGAGAAGACCAGAT+TGG	0.593974	8_3:+62940375	None:intergenic
AAGTAATATTGGAGGGATGA+AGG	0.472105	8_3:-62938645	Msa1301030:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACGCAAGAACATTAAAATG+AGG	+	chr8_3:62938592-62938611	None:intergenic	30.0%
TTAATGTTCTTGCGTTAGAT+CGG	-	chr8_3:62938596-62938615	Msa1301030:CDS	30.0%
TCCACATGATTAAGAATGAA+TGG	-	chr8_3:62938783-62938802	Msa1301030:intron	30.0%
CCACATGATTAAGAATGAAT+GGG	-	chr8_3:62938784-62938803	Msa1301030:intron	30.0%
CACATGATTAAGAATGAATG+GGG	-	chr8_3:62938785-62938804	Msa1301030:intron	30.0%
AAATACAGACATGAGATTGT+TGG	-	chr8_3:62938925-62938944	Msa1301030:intron	30.0%
TACATCACCTAACAAAGAAA+AGG	-	chr8_3:62939362-62939381	Msa1301030:CDS	30.0%
CAAATCAAACAAGAGATCTA+TGG	+	chr8_3:62939815-62939834	None:intergenic	30.0%
ATAGTAGCTGGAAGTAATAT+TGG	-	chr8_3:62939942-62939961	Msa1301030:intron	30.0%
TTGTGTAGAATTTAAGAGCT+TGG	+	chr8_3:62940131-62940150	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	62937976	62940644	62937976	ID=Msa1301030;Name=Msa1301030
chr8_3	mRNA	62937976	62940644	62937976	ID=Msa1301030-mRNA-1;Parent=Msa1301030;Name=Msa1301030-mRNA-1;_AED=0.05;_eAED=0.05;_QI=188\|1\|1\|1\|1\|1\|5\|505\|361
chr8_3	exon	62937976	62938686	62937976	ID=Msa1301030-mRNA-1:exon:18754;Parent=Msa1301030-mRNA-1
chr8_3	exon	62938934	62939087	62938934	ID=Msa1301030-mRNA-1:exon:18753;Parent=Msa1301030-mRNA-1
chr8_3	exon	62939174	62939687	62939174	ID=Msa1301030-mRNA-1:exon:18752;Parent=Msa1301030-mRNA-1
chr8_3	exon	62939786	62939899	62939786	ID=Msa1301030-mRNA-1:exon:18751;Parent=Msa1301030-mRNA-1
chr8_3	exon	62940359	62940644	62940359	ID=Msa1301030-mRNA-1:exon:18750;Parent=Msa1301030-mRNA-1
chr8_3	five_prime_UTR	62940457	62940644	62940457	ID=Msa1301030-mRNA-1:five_prime_utr;Parent=Msa1301030-mRNA-1
chr8_3	CDS	62940359	62940456	62940359	ID=Msa1301030-mRNA-1:cds;Parent=Msa1301030-mRNA-1
chr8_3	CDS	62939786	62939899	62939786	ID=Msa1301030-mRNA-1:cds;Parent=Msa1301030-mRNA-1
chr8_3	CDS	62939174	62939687	62939174	ID=Msa1301030-mRNA-1:cds;Parent=Msa1301030-mRNA-1
chr8_3	CDS	62938934	62939087	62938934	ID=Msa1301030-mRNA-1:cds;Parent=Msa1301030-mRNA-1
chr8_3	CDS	62938481	62938686	62938481	ID=Msa1301030-mRNA-1:cds;Parent=Msa1301030-mRNA-1
chr8_3	three_prime_UTR	62937976	62938480	62937976	ID=Msa1301030-mRNA-1:three_prime_utr;Parent=Msa1301030-mRNA-1

Gene Sequence

>Msa1301030

ATGGGATCACAACCTGAATTTGAGCATCCAAATGTGGCCTTTGGTTGGGCAGCAAGAGACCAATCTGGTCTTCTCTCCCCTTTCAATTTCTCCAGAAGGGAAACAAATGAAAAAGATGTGTCATTCAAAATATTGTTTTGTGGAATATGTCACACAGATCTCCACATGATTAAGAATGAATGGGGCATATCCACCTATCCACTAGTTCCTGGACATGAGATTGTTGGTATAGTGACAAAAGTGGGAAGCAAAGTAGAAAAGTTTAAAGTTGGAGACAAAGTGGGTGTTGGCTTCATGATCGATTCATGTCGATCATGTCAAAATTGTGCTGAAAATCTCGAAAGTTATTGTCCTCGCTTTACAGCCACGTGCGGTGGCAAATATCGTGATGACACCACCACATACGGAGGTTACTCTGACTCAATGGTTGTAGACGAGCACTTTGTGGTTCGCATTCCTGATGGTTTACCACTTGAAGCTGCTGCTCCTCTTCTTTGTGCCGGTATCACAGTGTATAGCCCTCTTCGATATTTTGGACTCGACAAGCCTGGTATGCATATAGGTGTTGTTGGTCTTGGTGGACTTGGTCACATGGCTGTTAAGTTTGCTAAAGCTTTTGGTGCTAATGTGACTGTGATTAGTACATCACCTAACAAAGAAAAGGAAGCAAGAGAACTCTTGGGAGCTGACTCATTTTTGATAAGTCGTGACCAAGATAAGATGCAAGCTTCAATGGATACATTAGACGGTATCATTGACACAGTTTCAGCGGAACATCCTCTCTCACCTCTAATTGGATTACTGAAGTCTCATGGAAAACTTGTAATGGTTGGTGCTCCAGCGAAGCCTCTCGAGCTCCCCGTCTTTACTTTACTTTCGGGGAGAAAGATAGTAGCTGGAAGTAATATTGGAGGGATGAAGGAGACTCAAGATATGATTGATTTTGCTGCCAAACACAATGTAAAGCCTACCATTGAAGTCATCCCTATGGATTATGTGAACACTGCAATGGAGCGACTCGTCAAAGCAGATGTCAAATATCGATTTGTGATTGACATTGGAAACACTCTCAAACCAAGCTCTTAA

Protein sequence

>Msa1301030

MGSQPEFEHPNVAFGWAARDQSGLLSPFNFSRRETNEKDVSFKILFCGICHTDLHMIKNEWGISTYPLVPGHEIVGIVTKVGSKVEKFKVGDKVGVGFMIDSCRSCQNCAENLESYCPRFTATCGGKYRDDTTTYGGYSDSMVVDEHFVVRIPDGLPLEAAAPLLCAGITVYSPLRYFGLDKPGMHIGVVGLGGLGHMAVKFAKAFGANVTVISTSPNKEKEARELLGADSFLISRDQDKMQASMDTLDGIIDTVSAEHPLSPLIGLLKSHGKLVMVGAPAKPLELPVFTLLSGRKIVAGSNIGGMKETQDMIDFAAKHNVKPTIEVIPMDYVNTAMERLVKADVKYRFVIDIGNTLKPSS*