AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1313220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1313220 MtrunA17_Chr8g0341741 96.715 274 9 0 1 274 1 274 0.0 551
Msa1313220 MtrunA17_Chr8g0341731 87.956 274 33 0 1 274 1 274 0.0 508
Msa1313220 MtrunA17_Chr8g0341701 83.942 274 43 1 1 274 1 273 3.96e-176 486
Msa1313220 MtrunA17_Chr8g0341721 83.209 268 40 2 1 264 1 267 1.67e-167 464
Msa1313220 MtrunA17_Chr2g0330511 71.375 269 75 2 4 272 3 269 5.45e-143 402
Msa1313220 MtrunA17_Chr2g0309111 69.888 269 80 1 4 272 47 314 5.45e-138 391
Msa1313220 MtrunA17_Chr2g0330491 57.353 272 111 2 5 272 4 274 8.43e-114 328
Msa1313220 MtrunA17_Chr8g0389271 57.384 237 97 4 5 239 2 236 1.26e-98 288
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1313220 AT1G17710.1 64.338 272 88 4 3 266 5 275 2.29e-129 368
Msa1313220 AT1G73010.1 65.385 260 85 2 5 259 14 273 8.68e-125 357
Msa1313220 AT1G17710.2 58.696 230 86 4 45 266 15 243 6.92e-96 282
Msa1313220 AT4G29530.1 52.282 241 113 2 5 244 4 243 4.83e-90 267
Msa1313220 AT4G29530.2 50.505 198 96 2 48 244 36 232 6.21e-67 208

Find 57 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAGCAAGCCTAGAATTTA+TGG 0.189661 8_4:+11033027 None:intergenic
TCTGAAAGTGGTTTCAAAAT+TGG 0.194557 8_4:+11034289 None:intergenic
CAAAGATACTTCACTTATTA+AGG 0.218927 8_4:-11033217 Msa1313220:CDS
GCTATCATTGCAAATGTAAT+AGG 0.269161 8_4:-11033002 Msa1313220:three_prime_UTR
AATTGGTTGATTGATGAATT+AGG 0.292483 8_4:-11034114 Msa1313220:CDS
ATTAAATCAGCACATGCATT+AGG 0.305553 8_4:-11033821 Msa1313220:CDS
ACAAGAAATAGAAGCTAAAT+TGG 0.312103 8_4:+11034264 None:intergenic
TCAGTTAGGCCATAAATTCT+AGG 0.325129 8_4:-11033036 Msa1313220:exon
TTGATTGTGACAGTGATAAT+TGG 0.327162 8_4:-11034131 Msa1313220:CDS
CTTATTAAGGCTGAAATTCA+TGG 0.328180 8_4:-11033204 Msa1313220:CDS
ATATATACCTTGCACATGTT+TGG 0.342939 8_4:+11033608 None:intergenic
GGTATTATTCTATGATGAAT+TGG 0.354716 8_4:+11033848 None:intergenic
ATGATGGAGTTTCATTCAAA+TGG 0.387824 8_4:-11033911 Msa1313220:CDS
TTTGATTAACAAAATTATGA+TGG 0.399120 8_4:-11033142 Msa1313220:CDS
GAACTGGACTAGATAGAGTT+TGG 0.404345 8_4:+11033082 None:intergenic
ATCTACCTTGGTGATGGTGC+TGG 0.412159 8_4:-11033321 Msa1313220:CDS
AGCTTCTTCCCACAATGCCT+TGG 0.420250 8_4:-11034071 Msa1313220:CDS
CCTTCTTGATTAACATAACC+AGG 0.424126 8_4:+11033698 None:intergenic
TTTATAGTAAGAGAAGGTAT+TGG 0.424163 8_4:+11034350 None:intergenic
TGATTGTGCTGGTTAATGAT+TGG 0.427338 8_4:-11032911 Msa1313220:three_prime_UTR
ATGCATTAGGGTGTGATTTG+AGG 0.444003 8_4:-11033808 Msa1313220:CDS
GTGAAGCATTATTGAAATCA+TGG 0.446007 8_4:+11033654 None:intergenic
CACACTGGAAAATTCTTCCT+TGG 0.458582 8_4:+11033252 None:intergenic
TTAAATCAGCACATGCATTA+GGG 0.465493 8_4:-11033820 Msa1313220:CDS
TGCCTAAAGCTGTCTCAGTT+AGG 0.466799 8_4:-11033050 Msa1313220:CDS
ACAGAGTTCCAAGGCATTGT+GGG 0.466896 8_4:+11034063 None:intergenic
TAAACGTTCCTTGATTGTGC+TGG 0.473070 8_4:-11032922 Msa1313220:three_prime_UTR
TTAGGCAAAGATACAAGAAC+TGG 0.474583 8_4:+11033066 None:intergenic
ATCACTGTCACAATCAATGA+TGG 0.486684 8_4:+11034137 None:intergenic
CTTTCTCTTAGCCTCAAGCT+AGG 0.488657 8_4:+11033288 None:intergenic
GCATGTGCTGATTTAATAGC+AGG 0.497078 8_4:+11033827 None:intergenic
GCTAAATTGGTATCTGAAAG+TGG 0.498277 8_4:+11034277 None:intergenic
CCTGGTTATGTTAATCAAGA+AGG 0.499879 8_4:-11033698 Msa1313220:CDS
TATACCTTGCACATGTTTGG+AGG 0.502608 8_4:+11033611 None:intergenic
GGCCTAACTGAGACAGCTTT+AGG 0.505096 8_4:+11033048 None:intergenic
TCAGAGATTAACACTAATCC+TGG 0.509786 8_4:-11033716 Msa1313220:CDS
GAAATTCATGGTTGGTGTGA+TGG 0.515965 8_4:-11033192 Msa1313220:CDS
ATTATAGGACAAGATGATGA+TGG 0.517951 8_4:-11033927 Msa1313220:intron
TATGATACAGAGAGTGAGAG+AGG 0.521370 8_4:+11034325 None:intergenic
ATCATTAACCAGCACAATCA+AGG 0.530531 8_4:+11032914 None:intergenic
GAGGTATTTATAGTAAGAGA+AGG 0.550239 8_4:+11034344 None:intergenic
GATTAACAAAATTATGATGG+AGG 0.550625 8_4:-11033139 Msa1313220:CDS
GACAGAGTTCCAAGGCATTG+TGG 0.554836 8_4:+11034062 None:intergenic
TTGGTGTGATGGAGAAGAGT+TGG 0.555513 8_4:-11033181 Msa1313220:CDS
AGGTTTATCTACCTTGGTGA+TGG 0.556828 8_4:-11033327 Msa1313220:CDS
TTTCTCTTAGCCTCAAGCTA+GGG 0.559791 8_4:+11033289 None:intergenic
GGTTTATTAAAGTTAATCAA+AGG 0.562569 8_4:+11032967 None:intergenic
TTAAGGCTGAAATTCATGGT+TGG 0.568918 8_4:-11033200 Msa1313220:CDS
GATTTCAATAATGCTTCACA+TGG 0.591506 8_4:-11033650 Msa1313220:CDS
GCATTATTGAAATCATGGTA+AGG 0.593751 8_4:+11033659 None:intergenic
GAGACTATTGCCCTAGCTTG+AGG 0.597383 8_4:-11033299 Msa1313220:CDS
ATACCTTGCACATGTTTGGA+GGG 0.605181 8_4:+11033612 None:intergenic
TTGCATATCAAATCCCACAC+TGG 0.630294 8_4:+11033237 None:intergenic
CTGCCCTCCAAACATGTGCA+AGG 0.634726 8_4:-11033615 Msa1313220:intron
AATGCCTTGGAACTCTGTCA+TGG 0.634751 8_4:-11034058 Msa1313220:intron
CATACCATGACAGAGTTCCA+AGG 0.665251 8_4:+11034054 None:intergenic
AGTCTCCAGCACCATCACCA+AGG 0.694728 8_4:+11033316 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAAATCATTATTTC+AGG - chr8_4:11033852-11033871 Msa1313220:CDS 10.0%
!!! TTTTTAAAATCATTATTTCA+GGG - chr8_4:11033853-11033872 Msa1313220:CDS 10.0%
!!! TTTTTTAAAATCATTATTTC+AGG - chr8_4:11033852-11033871 Msa1313220:CDS 10.0%
!!! TTTTTAAAATCATTATTTCA+GGG - chr8_4:11033853-11033872 Msa1313220:CDS 10.0%
!!! TAATATATGATTAACTTGTT+TGG - chr8_4:11033295-11033314 Msa1313220:CDS 15.0%
!! ATTAAAGAATAATTTGATGT+AGG - chr8_4:11033724-11033743 Msa1313220:CDS 15.0%
!! TTTGATTAACAAAATTATGA+TGG - chr8_4:11034110-11034129 Msa1313220:CDS 15.0%
!!! TAATATATGATTAACTTGTT+TGG - chr8_4:11033295-11033314 Msa1313220:CDS 15.0%
!! ATTAAAGAATAATTTGATGT+AGG - chr8_4:11033724-11033743 Msa1313220:CDS 15.0%
!! TTTGATTAACAAAATTATGA+TGG - chr8_4:11034110-11034129 Msa1313220:CDS 15.0%
!!! TGCAAGGTATATATTTTTTT+GGG - chr8_4:11033653-11033672 Msa1313220:CDS 20.0%
!! GGTTTATTAAAGTTAATCAA+AGG + chr8_4:11034288-11034307 None:intergenic 20.0%
!!! TGCAAGGTATATATTTTTTT+GGG - chr8_4:11033653-11033672 Msa1313220:CDS 20.0%
!! GGTTTATTAAAGTTAATCAA+AGG + chr8_4:11034288-11034307 None:intergenic 20.0%
!! TTTATAGTAAGAGAAGGTAT+TGG + chr8_4:11032905-11032924 None:intergenic 25.0%
! ACAAGAAATAGAAGCTAAAT+TGG + chr8_4:11032991-11033010 None:intergenic 25.0%
! AATTGGTTGATTGATGAATT+AGG - chr8_4:11033138-11033157 Msa1313220:CDS 25.0%
!!! ATGATTAACTTGTTTGGTTT+TGG - chr8_4:11033301-11033320 Msa1313220:CDS 25.0%
!!! TTGTTTGGTTTTGGAATTAT+AGG - chr8_4:11033310-11033329 Msa1313220:CDS 25.0%
!!! AACTTTTTCAATCTCTTCAA+TGG + chr8_4:11033372-11033391 None:intergenic 25.0%
! AGATTGAAAAAGTTCTTCAA+AGG - chr8_4:11033378-11033397 Msa1313220:CDS 25.0%
! GGTATTATTCTATGATGAAT+TGG + chr8_4:11033407-11033426 None:intergenic 25.0%
!!! TGAAACTATTTTGAAGCATT+TGG - chr8_4:11033496-11033515 Msa1313220:intron 25.0%
!!! GAAACTATTTTGAAGCATTT+GGG - chr8_4:11033497-11033516 Msa1313220:intron 25.0%
!!! GTGCAAGGTATATATTTTTT+TGG - chr8_4:11033652-11033671 Msa1313220:CDS 25.0%
! TATATTGGCATAAAATCTAG+TGG + chr8_4:11033703-11033722 None:intergenic 25.0%
!! CCTCTTCAAAAATTGAATTT+TGG + chr8_4:11033897-11033916 None:intergenic 25.0%
!!! CCAAAATTCAATTTTTGAAG+AGG - chr8_4:11033894-11033913 Msa1313220:CDS 25.0%
!!! TTTTTGAAGAGGATAAAAAG+AGG - chr8_4:11033905-11033924 Msa1313220:CDS 25.0%
! CAAAGATACTTCACTTATTA+AGG - chr8_4:11034035-11034054 Msa1313220:intron 25.0%
! GATTAACAAAATTATGATGG+AGG - chr8_4:11034113-11034132 Msa1313220:CDS 25.0%
!! ACCTTTTCTTGTACTAAAAT+TGG - chr8_4:11034305-11034324 Msa1313220:five_prime_UTR 25.0%
!!! ACCAATTTTAGTACAAGAAA+AGG + chr8_4:11034309-11034328 None:intergenic 25.0%
!!! ATCACGTTTTTTTTTTCCTT+TGG - chr8_4:11034369-11034388 Msa1313220:five_prime_UTR 25.0%
!! TTTATAGTAAGAGAAGGTAT+TGG + chr8_4:11032905-11032924 None:intergenic 25.0%
! ACAAGAAATAGAAGCTAAAT+TGG + chr8_4:11032991-11033010 None:intergenic 25.0%
! AATTGGTTGATTGATGAATT+AGG - chr8_4:11033138-11033157 Msa1313220:CDS 25.0%
!!! ATGATTAACTTGTTTGGTTT+TGG - chr8_4:11033301-11033320 Msa1313220:CDS 25.0%
!!! TTGTTTGGTTTTGGAATTAT+AGG - chr8_4:11033310-11033329 Msa1313220:CDS 25.0%
!!! AACTTTTTCAATCTCTTCAA+TGG + chr8_4:11033372-11033391 None:intergenic 25.0%
! AGATTGAAAAAGTTCTTCAA+AGG - chr8_4:11033378-11033397 Msa1313220:CDS 25.0%
! GGTATTATTCTATGATGAAT+TGG + chr8_4:11033407-11033426 None:intergenic 25.0%
!!! TGAAACTATTTTGAAGCATT+TGG - chr8_4:11033496-11033515 Msa1313220:intron 25.0%
!!! GAAACTATTTTGAAGCATTT+GGG - chr8_4:11033497-11033516 Msa1313220:intron 25.0%
!!! GTGCAAGGTATATATTTTTT+TGG - chr8_4:11033652-11033671 Msa1313220:CDS 25.0%
! TATATTGGCATAAAATCTAG+TGG + chr8_4:11033703-11033722 None:intergenic 25.0%
!! CCTCTTCAAAAATTGAATTT+TGG + chr8_4:11033897-11033916 None:intergenic 25.0%
!!! CCAAAATTCAATTTTTGAAG+AGG - chr8_4:11033894-11033913 Msa1313220:CDS 25.0%
!!! TTTTTGAAGAGGATAAAAAG+AGG - chr8_4:11033905-11033924 Msa1313220:CDS 25.0%
! CAAAGATACTTCACTTATTA+AGG - chr8_4:11034035-11034054 Msa1313220:intron 25.0%
! GATTAACAAAATTATGATGG+AGG - chr8_4:11034113-11034132 Msa1313220:CDS 25.0%
!! ACCTTTTCTTGTACTAAAAT+TGG - chr8_4:11034305-11034324 Msa1313220:five_prime_UTR 25.0%
!!! ACCAATTTTAGTACAAGAAA+AGG + chr8_4:11034309-11034328 None:intergenic 25.0%
! GAGGTATTTATAGTAAGAGA+AGG + chr8_4:11032911-11032930 None:intergenic 30.0%
!!! TTCAAAATTGGTTTTTGGCT+TGG + chr8_4:11032954-11032973 None:intergenic 30.0%
!!! GTGGTTTCAAAATTGGTTTT+TGG + chr8_4:11032959-11032978 None:intergenic 30.0%
TCTGAAAGTGGTTTCAAAAT+TGG + chr8_4:11032966-11032985 None:intergenic 30.0%
!!! TGAACCAGTTTTTTCTTTCT+GGG - chr8_4:11033010-11033029 Msa1313220:three_prime_UTR 30.0%
!!! TAGTCATTTTTCTCTTTGAG+AGG + chr8_4:11033062-11033081 None:intergenic 30.0%
TTGATTGTGACAGTGATAAT+TGG - chr8_4:11033121-11033140 Msa1313220:CDS 30.0%
ATTATAGGACAAGATGATGA+TGG - chr8_4:11033325-11033344 Msa1313220:CDS 30.0%
! ATGATGGAGTTTCATTCAAA+TGG - chr8_4:11033341-11033360 Msa1313220:CDS 30.0%
ATTAAATCAGCACATGCATT+AGG - chr8_4:11033431-11033450 Msa1313220:intron 30.0%
TTAAATCAGCACATGCATTA+GGG - chr8_4:11033432-11033451 Msa1313220:intron 30.0%
GCATTATTGAAATCATGGTA+AGG + chr8_4:11033596-11033615 None:intergenic 30.0%
! GTGAAGCATTATTGAAATCA+TGG + chr8_4:11033601-11033620 None:intergenic 30.0%
GATTTCAATAATGCTTCACA+TGG - chr8_4:11033602-11033621 Msa1313220:intron 30.0%
ATATATACCTTGCACATGTT+TGG + chr8_4:11033647-11033666 None:intergenic 30.0%
TCTTCATGAATTCAAACTAG+TGG + chr8_4:11033751-11033770 None:intergenic 30.0%
AAAAAGAGGTTTATCTACCT+TGG - chr8_4:11033919-11033938 Msa1313220:CDS 30.0%
CTTATTAAGGCTGAAATTCA+TGG - chr8_4:11034048-11034067 Msa1313220:intron 30.0%
GCTATCATTGCAAATGTAAT+AGG - chr8_4:11034250-11034269 Msa1313220:five_prime_UTR 30.0%
! GAGGTATTTATAGTAAGAGA+AGG + chr8_4:11032911-11032930 None:intergenic 30.0%
!!! TTCAAAATTGGTTTTTGGCT+TGG + chr8_4:11032954-11032973 None:intergenic 30.0%
!!! GTGGTTTCAAAATTGGTTTT+TGG + chr8_4:11032959-11032978 None:intergenic 30.0%
TCTGAAAGTGGTTTCAAAAT+TGG + chr8_4:11032966-11032985 None:intergenic 30.0%
!!! TGAACCAGTTTTTTCTTTCT+GGG - chr8_4:11033010-11033029 Msa1313220:three_prime_UTR 30.0%
!!! TAGTCATTTTTCTCTTTGAG+AGG + chr8_4:11033062-11033081 None:intergenic 30.0%
TTGATTGTGACAGTGATAAT+TGG - chr8_4:11033121-11033140 Msa1313220:CDS 30.0%
ATTATAGGACAAGATGATGA+TGG - chr8_4:11033325-11033344 Msa1313220:CDS 30.0%
! ATGATGGAGTTTCATTCAAA+TGG - chr8_4:11033341-11033360 Msa1313220:CDS 30.0%
ATTAAATCAGCACATGCATT+AGG - chr8_4:11033431-11033450 Msa1313220:intron 30.0%
TTAAATCAGCACATGCATTA+GGG - chr8_4:11033432-11033451 Msa1313220:intron 30.0%
GCATTATTGAAATCATGGTA+AGG + chr8_4:11033596-11033615 None:intergenic 30.0%
! GTGAAGCATTATTGAAATCA+TGG + chr8_4:11033601-11033620 None:intergenic 30.0%
GATTTCAATAATGCTTCACA+TGG - chr8_4:11033602-11033621 Msa1313220:intron 30.0%
ATATATACCTTGCACATGTT+TGG + chr8_4:11033647-11033666 None:intergenic 30.0%
TCTTCATGAATTCAAACTAG+TGG + chr8_4:11033751-11033770 None:intergenic 30.0%
AAAAAGAGGTTTATCTACCT+TGG - chr8_4:11033919-11033938 Msa1313220:CDS 30.0%
CTTATTAAGGCTGAAATTCA+TGG - chr8_4:11034048-11034067 Msa1313220:intron 30.0%
GCTATCATTGCAAATGTAAT+AGG - chr8_4:11034250-11034269 Msa1313220:five_prime_UTR 30.0%
GCTAAATTGGTATCTGAAAG+TGG + chr8_4:11032978-11032997 None:intergenic 35.0%
!!! GTGAACCAGTTTTTTCTTTC+TGG - chr8_4:11033009-11033028 Msa1313220:three_prime_UTR 35.0%
ATGACCCAGAAAGAAAAAAC+TGG + chr8_4:11033017-11033036 None:intergenic 35.0%
ATCACTGTCACAATCAATGA+TGG + chr8_4:11033118-11033137 None:intergenic 35.0%
TACAAAACAGAGCACATGAA+TGG + chr8_4:11033233-11033252 None:intergenic 35.0%
TCAGAGATTAACACTAATCC+TGG - chr8_4:11033536-11033555 Msa1313220:intron 35.0%
CCTTCTTGATTAACATAACC+AGG + chr8_4:11033557-11033576 None:intergenic 35.0%
CCTGGTTATGTTAATCAAGA+AGG - chr8_4:11033554-11033573 Msa1313220:intron 35.0%
! AAAGAGATTTTGTGATGCCA+AGG - chr8_4:11033983-11034002 Msa1313220:intron 35.0%
! AAGGAAGAATTTTCCAGTGT+GGG - chr8_4:11034002-11034021 Msa1313220:intron 35.0%
TTAAGGCTGAAATTCATGGT+TGG - chr8_4:11034052-11034071 Msa1313220:intron 35.0%
TTAGGCAAAGATACAAGAAC+TGG + chr8_4:11034189-11034208 None:intergenic 35.0%
TCAGTTAGGCCATAAATTCT+AGG - chr8_4:11034216-11034235 Msa1313220:five_prime_UTR 35.0%
ACTAGCAAGCCTAGAATTTA+TGG + chr8_4:11034228-11034247 None:intergenic 35.0%
ATCATTAACCAGCACAATCA+AGG + chr8_4:11034341-11034360 None:intergenic 35.0%
!! TGATTGTGCTGGTTAATGAT+TGG - chr8_4:11034341-11034360 Msa1313220:five_prime_UTR 35.0%
GCTAAATTGGTATCTGAAAG+TGG + chr8_4:11032978-11032997 None:intergenic 35.0%
!!! GTGAACCAGTTTTTTCTTTC+TGG - chr8_4:11033009-11033028 Msa1313220:three_prime_UTR 35.0%
ATGACCCAGAAAGAAAAAAC+TGG + chr8_4:11033017-11033036 None:intergenic 35.0%
ATCACTGTCACAATCAATGA+TGG + chr8_4:11033118-11033137 None:intergenic 35.0%
TACAAAACAGAGCACATGAA+TGG + chr8_4:11033233-11033252 None:intergenic 35.0%
TCAGAGATTAACACTAATCC+TGG - chr8_4:11033536-11033555 Msa1313220:intron 35.0%
CCTTCTTGATTAACATAACC+AGG + chr8_4:11033557-11033576 None:intergenic 35.0%
CCTGGTTATGTTAATCAAGA+AGG - chr8_4:11033554-11033573 Msa1313220:intron 35.0%
! AAAGAGATTTTGTGATGCCA+AGG - chr8_4:11033983-11034002 Msa1313220:intron 35.0%
! AAGGAAGAATTTTCCAGTGT+GGG - chr8_4:11034002-11034021 Msa1313220:intron 35.0%
TTAAGGCTGAAATTCATGGT+TGG - chr8_4:11034052-11034071 Msa1313220:intron 35.0%
TTAGGCAAAGATACAAGAAC+TGG + chr8_4:11034189-11034208 None:intergenic 35.0%
TCAGTTAGGCCATAAATTCT+AGG - chr8_4:11034216-11034235 Msa1313220:five_prime_UTR 35.0%
ACTAGCAAGCCTAGAATTTA+TGG + chr8_4:11034228-11034247 None:intergenic 35.0%
ATCATTAACCAGCACAATCA+AGG + chr8_4:11034341-11034360 None:intergenic 35.0%
!! TGATTGTGCTGGTTAATGAT+TGG - chr8_4:11034341-11034360 Msa1313220:five_prime_UTR 35.0%
TATGATACAGAGAGTGAGAG+AGG + chr8_4:11032930-11032949 None:intergenic 40.0%
!! GCATGTGCTGATTTAATAGC+AGG + chr8_4:11033428-11033447 None:intergenic 40.0%
ATGCATTAGGGTGTGATTTG+AGG - chr8_4:11033444-11033463 Msa1313220:intron 40.0%
ATACCTTGCACATGTTTGGA+GGG + chr8_4:11033643-11033662 None:intergenic 40.0%
TATACCTTGCACATGTTTGG+AGG + chr8_4:11033644-11033663 None:intergenic 40.0%
AGGTTTATCTACCTTGGTGA+TGG - chr8_4:11033925-11033944 Msa1313220:intron 40.0%
TTTCTCTTAGCCTCAAGCTA+GGG + chr8_4:11033966-11033985 None:intergenic 40.0%
CACACTGGAAAATTCTTCCT+TGG + chr8_4:11034003-11034022 None:intergenic 40.0%
! CAAGGAAGAATTTTCCAGTG+TGG - chr8_4:11034001-11034020 Msa1313220:intron 40.0%
TTGCATATCAAATCCCACAC+TGG + chr8_4:11034018-11034037 None:intergenic 40.0%
! GAAATTCATGGTTGGTGTGA+TGG - chr8_4:11034060-11034079 Msa1313220:CDS 40.0%
! GAACTGGACTAGATAGAGTT+TGG + chr8_4:11034173-11034192 None:intergenic 40.0%
TAAACGTTCCTTGATTGTGC+TGG - chr8_4:11034330-11034349 Msa1313220:five_prime_UTR 40.0%
TATGATACAGAGAGTGAGAG+AGG + chr8_4:11032930-11032949 None:intergenic 40.0%
!! GCATGTGCTGATTTAATAGC+AGG + chr8_4:11033428-11033447 None:intergenic 40.0%
ATGCATTAGGGTGTGATTTG+AGG - chr8_4:11033444-11033463 Msa1313220:intron 40.0%
ATACCTTGCACATGTTTGGA+GGG + chr8_4:11033643-11033662 None:intergenic 40.0%
TATACCTTGCACATGTTTGG+AGG + chr8_4:11033644-11033663 None:intergenic 40.0%
AGGTTTATCTACCTTGGTGA+TGG - chr8_4:11033925-11033944 Msa1313220:intron 40.0%
TTTCTCTTAGCCTCAAGCTA+GGG + chr8_4:11033966-11033985 None:intergenic 40.0%
CACACTGGAAAATTCTTCCT+TGG + chr8_4:11034003-11034022 None:intergenic 40.0%
! CAAGGAAGAATTTTCCAGTG+TGG - chr8_4:11034001-11034020 Msa1313220:intron 40.0%
TTGCATATCAAATCCCACAC+TGG + chr8_4:11034018-11034037 None:intergenic 40.0%
! GAAATTCATGGTTGGTGTGA+TGG - chr8_4:11034060-11034079 Msa1313220:CDS 40.0%
! GAACTGGACTAGATAGAGTT+TGG + chr8_4:11034173-11034192 None:intergenic 40.0%
TAAACGTTCCTTGATTGTGC+TGG - chr8_4:11034330-11034349 Msa1313220:five_prime_UTR 40.0%
!! ACAGAGTTCCAAGGCATTGT+GGG + chr8_4:11033192-11033211 None:intergenic 45.0%
AATGCCTTGGAACTCTGTCA+TGG - chr8_4:11033194-11033213 Msa1313220:CDS 45.0%
CATACCATGACAGAGTTCCA+AGG + chr8_4:11033201-11033220 None:intergenic 45.0%
CTTTCTCTTAGCCTCAAGCT+AGG + chr8_4:11033967-11033986 None:intergenic 45.0%
! TTGGTGTGATGGAGAAGAGT+TGG - chr8_4:11034071-11034090 Msa1313220:CDS 45.0%
TGCCTAAAGCTGTCTCAGTT+AGG - chr8_4:11034202-11034221 Msa1313220:five_prime_UTR 45.0%
!! ACAGAGTTCCAAGGCATTGT+GGG + chr8_4:11033192-11033211 None:intergenic 45.0%
AATGCCTTGGAACTCTGTCA+TGG - chr8_4:11033194-11033213 Msa1313220:CDS 45.0%
CATACCATGACAGAGTTCCA+AGG + chr8_4:11033201-11033220 None:intergenic 45.0%
CTTTCTCTTAGCCTCAAGCT+AGG + chr8_4:11033967-11033986 None:intergenic 45.0%
! TTGGTGTGATGGAGAAGAGT+TGG - chr8_4:11034071-11034090 Msa1313220:CDS 45.0%
TGCCTAAAGCTGTCTCAGTT+AGG - chr8_4:11034202-11034221 Msa1313220:five_prime_UTR 45.0%
!!! AATTATTCTTTAATTTATAT+TGG + chr8_4:11033718-11033737 None:intergenic 5.0%
!!! AATTATTCTTTAATTTATAT+TGG + chr8_4:11033718-11033737 None:intergenic 5.0%
AGCTTCTTCCCACAATGCCT+TGG - chr8_4:11033181-11033200 Msa1313220:CDS 50.0%
!! GACAGAGTTCCAAGGCATTG+TGG + chr8_4:11033193-11033212 None:intergenic 50.0%
!! ATCTACCTTGGTGATGGTGC+TGG - chr8_4:11033931-11033950 Msa1313220:intron 50.0%
GAGACTATTGCCCTAGCTTG+AGG - chr8_4:11033953-11033972 Msa1313220:intron 50.0%
GGCCTAACTGAGACAGCTTT+AGG + chr8_4:11034207-11034226 None:intergenic 50.0%
AGCTTCTTCCCACAATGCCT+TGG - chr8_4:11033181-11033200 Msa1313220:CDS 50.0%
!! GACAGAGTTCCAAGGCATTG+TGG + chr8_4:11033193-11033212 None:intergenic 50.0%
!! ATCTACCTTGGTGATGGTGC+TGG - chr8_4:11033931-11033950 Msa1313220:intron 50.0%
GAGACTATTGCCCTAGCTTG+AGG - chr8_4:11033953-11033972 Msa1313220:intron 50.0%
GGCCTAACTGAGACAGCTTT+AGG + chr8_4:11034207-11034226 None:intergenic 50.0%
CTGCCCTCCAAACATGTGCA+AGG - chr8_4:11033637-11033656 Msa1313220:CDS 55.0%
AGTCTCCAGCACCATCACCA+AGG + chr8_4:11033939-11033958 None:intergenic 55.0%
CTGCCCTCCAAACATGTGCA+AGG - chr8_4:11033637-11033656 Msa1313220:CDS 55.0%
AGTCTCCAGCACCATCACCA+AGG + chr8_4:11033939-11033958 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 11032886 11034388 11032886 ID=Msa1313220;Name=Msa1313220
chr8_4 mRNA 11032886 11034388 11032886 ID=Msa1313220-mRNA-1;Parent=Msa1313220;Name=Msa1313220-mRNA-1;_AED=0.05;_eAED=0.05;_QI=189|1|1|1|1|1|3|158|274
chr8_4 exon 11032886 11033400 11032886 ID=Msa1313220-mRNA-1:exon:4073;Parent=Msa1313220-mRNA-1
chr8_4 exon 11033616 11033942 11033616 ID=Msa1313220-mRNA-1:exon:4072;Parent=Msa1313220-mRNA-1
chr8_4 exon 11034059 11034388 11034059 ID=Msa1313220-mRNA-1:exon:4071;Parent=Msa1313220-mRNA-1
chr8_4 five_prime_UTR 11034200 11034388 11034200 ID=Msa1313220-mRNA-1:five_prime_utr;Parent=Msa1313220-mRNA-1
chr8_4 CDS 11034059 11034199 11034059 ID=Msa1313220-mRNA-1:cds;Parent=Msa1313220-mRNA-1
chr8_4 CDS 11033616 11033942 11033616 ID=Msa1313220-mRNA-1:cds;Parent=Msa1313220-mRNA-1
chr8_4 CDS 11033044 11033400 11033044 ID=Msa1313220-mRNA-1:cds;Parent=Msa1313220-mRNA-1
chr8_4 three_prime_UTR 11032886 11033043 11032886 ID=Msa1313220-mRNA-1:three_prime_utr;Parent=Msa1313220-mRNA-1
Gene Sequence

>Msa1313220

ATGACTAATAACATTGTTATTGTTTTCGACTTTGACAAAACCATCATTGATTGTGACAGTGATAATTGGTTGATTGATGAATTAGGTTTCACTGATTTGTTTAATCAGCTTCTTCCCACAATGCCTTGGAACTCTGTCATGGACAAGATGATGATGGAGTTTCATTCAAATGGAATAACCATTGAAGAGATTGAAAAAGTTCTTCAAAGGATTCCAATTCATCATAGAATAATACCTGCTATTAAATCAGCACATGCATTAGGGTGTGATTTGAGGATTGTTAGTGATGCTAATATGTTTTACATTGAAACTATTTTGAAGCATTTGGGAATAAGTGAATACTTCTCAGAGATTAACACTAATCCTGGTTATGTTAATCAAGAAGGAAGAGTTAGAATTTTTCCTTACCATGATTTCAATAATGCTTCACATGGTTGCAACAATGTCTGCCCTCCAAACATGTGCAAGGGTTTAATCATTGATAAAATCCAAAATTCAATTTTTGAAGAGGATAAAAAGAGGTTTATCTACCTTGGTGATGGTGCTGGAGACTATTGCCCTAGCTTGAGGCTAAGAGAAAGAGATTTTGTGATGCCAAGGAAGAATTTTCCAGTGTGGGATTTGATATGCAAAGATACTTCACTTATTAAGGCTGAAATTCATGGTTGGTGTGATGGAGAAGAGTTGGAACAAGTTTTGCTGAATTTGATTAACAAAATTATGATGGAGGAACATGTTCAACTTATTTCTTCTGATTGCAAGCTCCAAACTCTATCTAGTCCAGTTCTTGTATCTTTGCCTAAAGCTGTCTCAGTTAGGCCATAA
Protein sequence

>Msa1313220

MTNNIVIVFDFDKTIIDCDSDNWLIDELGFTDLFNQLLPTMPWNSVMDKMMMEFHSNGITIEEIEKVLQRIPIHHRIIPAIKSAHALGCDLRIVSDANMFYIETILKHLGISEYFSEINTNPGYVNQEGRVRIFPYHDFNNASHGCNNVCPPNMCKGLIIDKIQNSIFEEDKKRFIYLGDGAGDYCPSLRLRERDFVMPRKNFPVWDLICKDTSLIKAEIHGWCDGEELEQVLLNLINKIMMEEHVQLISSDCKLQTLSSPVLVSLPKAVSVRP*