AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1316390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1316390 MtrunA17_Chr8g0372221 94.118 85 5 0 1 85 1 85 4.28e-52 164
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATATAACAATGATTGTATC+TGG 0.187129 8_4:+16684455 Msa1316390:exon
CGAGGCAACGAGGAGGTAAT+TGG 0.227784 8_4:-16684505 None:intergenic
GCCTCGAATCACGCCGTGTT+CGG 0.266223 8_4:+16684522 Msa1316390:CDS
AGATTGAGGAAGCAGGGTTT+TGG 0.348685 8_4:+16683467 None:intergenic
CCTCGAATCACGCCGTGTTC+GGG 0.353082 8_4:+16684523 Msa1316390:CDS
TTGAGAATGCAGAGGGAAGA+AGG 0.377661 8_4:-16683516 None:intergenic
GAGGCAACGAGGAGGTAATT+GGG 0.389953 8_4:-16684504 None:intergenic
AGAGGGAAAGTGTTGTTGTT+GGG 0.426747 8_4:-16683564 None:intergenic
TGAGAATGCAGAGGGAAGAA+GGG 0.442821 8_4:-16683515 None:intergenic
AGTGCTATGGGTGCTTCATT+TGG 0.458480 8_4:+16684771 Msa1316390:CDS
AAGAGGGAAAGTGTTGTTGT+TGG 0.465680 8_4:-16683565 None:intergenic
TCTGCATTCTCAAAATCATC+AGG 0.473976 8_4:+16683526 Msa1316390:five_prime_UTR
TCAATTGGTCAGAAGTGCTA+TGG 0.489625 8_4:+16684758 Msa1316390:CDS
TGATAGACGACAAGTTCAAT+TGG 0.501549 8_4:+16684743 Msa1316390:CDS
ATATAACAATGATTGTATCT+GGG 0.516139 8_4:+16684456 Msa1316390:exon
TGGGCATACCTTTCCACACT+GGG 0.516470 8_4:-16684593 None:intergenic
AGGCAACGAGGAGGTAATTG+GGG 0.569451 8_4:-16684503 None:intergenic
AGGGATAAACACTTTCCAAA+GGG 0.569755 8_4:-16683496 None:intergenic
CAATTGGTCAGAAGTGCTAT+GGG 0.571474 8_4:+16684759 Msa1316390:CDS
TTGGGCATACCTTTCCACAC+TGG 0.594896 8_4:-16684594 None:intergenic
AAGGGATAAACACTTTCCAA+AGG 0.617286 8_4:-16683497 None:intergenic
AGAATAGAGAACACAAGTTG+AGG 0.637460 8_4:-16684390 None:intergenic
AAGGACAGCGTTTCCCAGTG+TGG 0.653221 8_4:+16684580 Msa1316390:CDS
GATATGTCAAATGATTCTGC+TGG 0.657645 8_4:+16684795 Msa1316390:CDS
TGGCTGGTAAATGTATACAA+AGG 0.664469 8_4:+16684561 Msa1316390:CDS
AAAATGACATGACCCGAACA+CGG 0.665683 8_4:-16684535 None:intergenic
ATGATTGTATCTGGGTTGAG+TGG 0.684151 8_4:+16684464 Msa1316390:CDS
GGCAACGAGGAGGTAATTGG+GGG 0.687219 8_4:-16684502 None:intergenic
CGGCGTGATTCGAGGCAACG+AGG 0.707572 8_4:-16684515 None:intergenic
CGTGATTCGAGGCAACGAGG+AGG 0.732059 8_4:-16684512 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAATTAAAAAAAAA+AGG - chr8_4:16683720-16683739 None:intergenic 0.0%
!! ATAAATAAATTAAAAAAAAA+AGG - chr8_4:16683720-16683739 None:intergenic 0.0%
!!! TTATTTTTATAGAATGAATT+TGG + chr8_4:16683842-16683861 Msa1316390:intron 10.0%
!!! TATTTTTATAGAATGAATTT+GGG + chr8_4:16683843-16683862 Msa1316390:intron 10.0%
!!! TTGTTATTTTTATATATTTG+AGG + chr8_4:16684238-16684257 Msa1316390:intron 10.0%
!!! TTATTTTTATAGAATGAATT+TGG + chr8_4:16683842-16683861 Msa1316390:intron 10.0%
!!! TATTTTTATAGAATGAATTT+GGG + chr8_4:16683843-16683862 Msa1316390:intron 10.0%
!!! TTGTTATTTTTATATATTTG+AGG + chr8_4:16684238-16684257 Msa1316390:intron 10.0%
!! TATTTATCATCATTTATACG+AGG + chr8_4:16683733-16683752 Msa1316390:intron 20.0%
!! TCATTCTATAAAAATAAGAG+CGG - chr8_4:16683839-16683858 None:intergenic 20.0%
!!! TTTTGTTAAGATTTTCACAT+TGG + chr8_4:16684045-16684064 Msa1316390:intron 20.0%
!! ATTGTTATATAAAAATCTCC+AGG - chr8_4:16684446-16684465 None:intergenic 20.0%
!! TATATAACAATGATTGTATC+TGG + chr8_4:16684455-16684474 Msa1316390:exon 20.0%
!! ATATAACAATGATTGTATCT+GGG + chr8_4:16684456-16684475 Msa1316390:exon 20.0%
!! TCAAAAAGTAATGATTCATT+GGG - chr8_4:16684615-16684634 None:intergenic 20.0%
!! TTCAAAAAGTAATGATTCAT+TGG - chr8_4:16684616-16684635 None:intergenic 20.0%
!! TATTTATCATCATTTATACG+AGG + chr8_4:16683733-16683752 Msa1316390:intron 20.0%
!! TCATTCTATAAAAATAAGAG+CGG - chr8_4:16683839-16683858 None:intergenic 20.0%
!!! TTTTGTTAAGATTTTCACAT+TGG + chr8_4:16684045-16684064 Msa1316390:intron 20.0%
!! ATTGTTATATAAAAATCTCC+AGG - chr8_4:16684446-16684465 None:intergenic 20.0%
!! TATATAACAATGATTGTATC+TGG + chr8_4:16684455-16684474 Msa1316390:exon 20.0%
!! ATATAACAATGATTGTATCT+GGG + chr8_4:16684456-16684475 Msa1316390:exon 20.0%
!! TCAAAAAGTAATGATTCATT+GGG - chr8_4:16684615-16684634 None:intergenic 20.0%
!! TTCAAAAAGTAATGATTCAT+TGG - chr8_4:16684616-16684635 None:intergenic 20.0%
!! CTATAATCTTATGTTTTTCG+TGG + chr8_4:16683922-16683941 Msa1316390:intron 25.0%
! ACAACAACATATTCTAGTAT+TGG - chr8_4:16683949-16683968 None:intergenic 25.0%
! ACATTGGATATGTTTATAAG+TGG + chr8_4:16684061-16684080 Msa1316390:intron 25.0%
! CATTGGATATGTTTATAAGT+GGG + chr8_4:16684062-16684081 Msa1316390:intron 25.0%
! ATTGGATATGTTTATAAGTG+GGG + chr8_4:16684063-16684082 Msa1316390:intron 25.0%
! ATATATTTGAGGAACTAAGT+AGG + chr8_4:16684249-16684268 Msa1316390:intron 25.0%
! CAGGTAAACAAAGATAAATA+TGG - chr8_4:16684328-16684347 None:intergenic 25.0%
!!! GAATCATTACTTTTTGAACT+TGG + chr8_4:16684618-16684637 Msa1316390:intron 25.0%
!! CTATAATCTTATGTTTTTCG+TGG + chr8_4:16683922-16683941 Msa1316390:intron 25.0%
! ACAACAACATATTCTAGTAT+TGG - chr8_4:16683949-16683968 None:intergenic 25.0%
! ACATTGGATATGTTTATAAG+TGG + chr8_4:16684061-16684080 Msa1316390:intron 25.0%
! CATTGGATATGTTTATAAGT+GGG + chr8_4:16684062-16684081 Msa1316390:intron 25.0%
! ATTGGATATGTTTATAAGTG+GGG + chr8_4:16684063-16684082 Msa1316390:intron 25.0%
! ATATATTTGAGGAACTAAGT+AGG + chr8_4:16684249-16684268 Msa1316390:intron 25.0%
! CAGGTAAACAAAGATAAATA+TGG - chr8_4:16684328-16684347 None:intergenic 25.0%
!!! GAATCATTACTTTTTGAACT+TGG + chr8_4:16684618-16684637 Msa1316390:intron 25.0%
ATGCAAATTTAGCATAAGCA+AGG - chr8_4:16683977-16683996 None:intergenic 30.0%
! ACAAATTGAGCAGTGTTTTA+TGG + chr8_4:16684018-16684037 Msa1316390:intron 30.0%
!! ATTATCTTCGGTGTCAATTT+AGG - chr8_4:16684134-16684153 None:intergenic 30.0%
TTAGTTTAGGGATTAAATCG+AGG + chr8_4:16684282-16684301 Msa1316390:intron 30.0%
TAGTTTAGGGATTAAATCGA+GGG + chr8_4:16684283-16684302 Msa1316390:intron 30.0%
TCACAAAGCAAAAACAGATA+GGG + chr8_4:16684412-16684431 Msa1316390:five_prime_UTR 30.0%
TGAACTTGGAACTTTATGTA+AGG + chr8_4:16684632-16684651 Msa1316390:intron 30.0%
!! AAGGTTCATGTTTTGTTGAA+TGG + chr8_4:16684651-16684670 Msa1316390:intron 30.0%
TAATCTAGGAGATGAAAAAC+TGG - chr8_4:16684718-16684737 None:intergenic 30.0%
!! CGTCTATCACATTTTAATCT+AGG - chr8_4:16684732-16684751 None:intergenic 30.0%
ATGCAAATTTAGCATAAGCA+AGG - chr8_4:16683977-16683996 None:intergenic 30.0%
! ACAAATTGAGCAGTGTTTTA+TGG + chr8_4:16684018-16684037 Msa1316390:intron 30.0%
!! ATTATCTTCGGTGTCAATTT+AGG - chr8_4:16684134-16684153 None:intergenic 30.0%
TTAGTTTAGGGATTAAATCG+AGG + chr8_4:16684282-16684301 Msa1316390:intron 30.0%
TAGTTTAGGGATTAAATCGA+GGG + chr8_4:16684283-16684302 Msa1316390:intron 30.0%
TCACAAAGCAAAAACAGATA+GGG + chr8_4:16684412-16684431 Msa1316390:five_prime_UTR 30.0%
TGAACTTGGAACTTTATGTA+AGG + chr8_4:16684632-16684651 Msa1316390:intron 30.0%
!! AAGGTTCATGTTTTGTTGAA+TGG + chr8_4:16684651-16684670 Msa1316390:intron 30.0%
TAATCTAGGAGATGAAAAAC+TGG - chr8_4:16684718-16684737 None:intergenic 30.0%
!! CGTCTATCACATTTTAATCT+AGG - chr8_4:16684732-16684751 None:intergenic 30.0%
AGGGATAAACACTTTCCAAA+GGG - chr8_4:16683499-16683518 None:intergenic 35.0%
AAGGGATAAACACTTTCCAA+AGG - chr8_4:16683500-16683519 None:intergenic 35.0%
!! GATGATTTTGAGAATGCAGA+GGG - chr8_4:16683526-16683545 None:intergenic 35.0%
!! TGATGATTTTGAGAATGCAG+AGG - chr8_4:16683527-16683546 None:intergenic 35.0%
TCTGCATTCTCAAAATCATC+AGG + chr8_4:16683526-16683545 Msa1316390:five_prime_UTR 35.0%
TTCGGCGTTAGAAAAAAAGA+GGG - chr8_4:16683584-16683603 None:intergenic 35.0%
!! ATACGAGAAGTTGATAACAG+TGG - chr8_4:16683621-16683640 None:intergenic 35.0%
! ATCGATTCTGTCGTTTATGT+GGG + chr8_4:16683780-16683799 Msa1316390:intron 35.0%
!! TTTGTTGAGTTGAACACACT+AGG + chr8_4:16683877-16683896 Msa1316390:intron 35.0%
! GTCTCTGGTTCAATTATCTT+CGG - chr8_4:16684146-16684165 None:intergenic 35.0%
TAATTGAACCAGAGACTTTG+AGG + chr8_4:16684150-16684169 Msa1316390:intron 35.0%
AGAATAGAGAACACAAGTTG+AGG - chr8_4:16684393-16684412 None:intergenic 35.0%
CTCACAAAGCAAAAACAGAT+AGG + chr8_4:16684411-16684430 Msa1316390:five_prime_UTR 35.0%
!!! ACAGATAGGGTTTTATTTCC+TGG + chr8_4:16684425-16684444 Msa1316390:five_prime_UTR 35.0%
TGGCTGGTAAATGTATACAA+AGG + chr8_4:16684561-16684580 Msa1316390:CDS 35.0%
TTCTGTAAATTGACAGGGTA+AGG - chr8_4:16684695-16684714 None:intergenic 35.0%
!! ACTGGTTCTGTAAATTGACA+GGG - chr8_4:16684700-16684719 None:intergenic 35.0%
!! AACTGGTTCTGTAAATTGAC+AGG - chr8_4:16684701-16684720 None:intergenic 35.0%
TGATAGACGACAAGTTCAAT+TGG + chr8_4:16684743-16684762 Msa1316390:CDS 35.0%
GATATGTCAAATGATTCTGC+TGG + chr8_4:16684795-16684814 Msa1316390:CDS 35.0%
AGGGATAAACACTTTCCAAA+GGG - chr8_4:16683499-16683518 None:intergenic 35.0%
AAGGGATAAACACTTTCCAA+AGG - chr8_4:16683500-16683519 None:intergenic 35.0%
!! GATGATTTTGAGAATGCAGA+GGG - chr8_4:16683526-16683545 None:intergenic 35.0%
!! TGATGATTTTGAGAATGCAG+AGG - chr8_4:16683527-16683546 None:intergenic 35.0%
TCTGCATTCTCAAAATCATC+AGG + chr8_4:16683526-16683545 Msa1316390:five_prime_UTR 35.0%
TTCGGCGTTAGAAAAAAAGA+GGG - chr8_4:16683584-16683603 None:intergenic 35.0%
!! ATACGAGAAGTTGATAACAG+TGG - chr8_4:16683621-16683640 None:intergenic 35.0%
! ATCGATTCTGTCGTTTATGT+GGG + chr8_4:16683780-16683799 Msa1316390:intron 35.0%
!! TTTGTTGAGTTGAACACACT+AGG + chr8_4:16683877-16683896 Msa1316390:intron 35.0%
! GTCTCTGGTTCAATTATCTT+CGG - chr8_4:16684146-16684165 None:intergenic 35.0%
TAATTGAACCAGAGACTTTG+AGG + chr8_4:16684150-16684169 Msa1316390:intron 35.0%
AGAATAGAGAACACAAGTTG+AGG - chr8_4:16684393-16684412 None:intergenic 35.0%
CTCACAAAGCAAAAACAGAT+AGG + chr8_4:16684411-16684430 Msa1316390:five_prime_UTR 35.0%
!!! ACAGATAGGGTTTTATTTCC+TGG + chr8_4:16684425-16684444 Msa1316390:five_prime_UTR 35.0%
TGGCTGGTAAATGTATACAA+AGG + chr8_4:16684561-16684580 Msa1316390:CDS 35.0%
TTCTGTAAATTGACAGGGTA+AGG - chr8_4:16684695-16684714 None:intergenic 35.0%
!! ACTGGTTCTGTAAATTGACA+GGG - chr8_4:16684700-16684719 None:intergenic 35.0%
!! AACTGGTTCTGTAAATTGAC+AGG - chr8_4:16684701-16684720 None:intergenic 35.0%
TGATAGACGACAAGTTCAAT+TGG + chr8_4:16684743-16684762 Msa1316390:CDS 35.0%
GATATGTCAAATGATTCTGC+TGG + chr8_4:16684795-16684814 Msa1316390:CDS 35.0%
!! AGAGGGAAAGTGTTGTTGTT+GGG - chr8_4:16683567-16683586 None:intergenic 40.0%
!! AAGAGGGAAAGTGTTGTTGT+TGG - chr8_4:16683568-16683587 None:intergenic 40.0%
CTTCGGCGTTAGAAAAAAAG+AGG - chr8_4:16683585-16683604 None:intergenic 40.0%
!!! CTCTTTTTTTCTAACGCCGA+AGG + chr8_4:16683583-16683602 Msa1316390:five_prime_UTR 40.0%
GCAGTAGAAGAGAATGAAAG+TGG - chr8_4:16683665-16683684 None:intergenic 40.0%
GACAGAATCGATGCAATTTG+AGG - chr8_4:16683772-16683791 None:intergenic 40.0%
!! CATCGATTCTGTCGTTTATG+TGG + chr8_4:16683779-16683798 Msa1316390:intron 40.0%
GAGTTGAACACACTAGGATA+TGG + chr8_4:16683883-16683902 Msa1316390:intron 40.0%
!! TCTTCGGTGTCAATTTAGGT+TGG - chr8_4:16684130-16684149 None:intergenic 40.0%
GAGAACTGATGAACAACATC+AGG - chr8_4:16684347-16684366 None:intergenic 40.0%
! ATGATTGTATCTGGGTTGAG+TGG + chr8_4:16684464-16684483 Msa1316390:CDS 40.0%
AAAATGACATGACCCGAACA+CGG - chr8_4:16684538-16684557 None:intergenic 40.0%
!! TCAATTGGTCAGAAGTGCTA+TGG + chr8_4:16684758-16684777 Msa1316390:CDS 40.0%
!! CAATTGGTCAGAAGTGCTAT+GGG + chr8_4:16684759-16684778 Msa1316390:CDS 40.0%
!! AGAGGGAAAGTGTTGTTGTT+GGG - chr8_4:16683567-16683586 None:intergenic 40.0%
!! AAGAGGGAAAGTGTTGTTGT+TGG - chr8_4:16683568-16683587 None:intergenic 40.0%
CTTCGGCGTTAGAAAAAAAG+AGG - chr8_4:16683585-16683604 None:intergenic 40.0%
!!! CTCTTTTTTTCTAACGCCGA+AGG + chr8_4:16683583-16683602 Msa1316390:five_prime_UTR 40.0%
GCAGTAGAAGAGAATGAAAG+TGG - chr8_4:16683665-16683684 None:intergenic 40.0%
GACAGAATCGATGCAATTTG+AGG - chr8_4:16683772-16683791 None:intergenic 40.0%
!! CATCGATTCTGTCGTTTATG+TGG + chr8_4:16683779-16683798 Msa1316390:intron 40.0%
GAGTTGAACACACTAGGATA+TGG + chr8_4:16683883-16683902 Msa1316390:intron 40.0%
!! TCTTCGGTGTCAATTTAGGT+TGG - chr8_4:16684130-16684149 None:intergenic 40.0%
GAGAACTGATGAACAACATC+AGG - chr8_4:16684347-16684366 None:intergenic 40.0%
! ATGATTGTATCTGGGTTGAG+TGG + chr8_4:16684464-16684483 Msa1316390:CDS 40.0%
AAAATGACATGACCCGAACA+CGG - chr8_4:16684538-16684557 None:intergenic 40.0%
!! TCAATTGGTCAGAAGTGCTA+TGG + chr8_4:16684758-16684777 Msa1316390:CDS 40.0%
!! CAATTGGTCAGAAGTGCTAT+GGG + chr8_4:16684759-16684778 Msa1316390:CDS 40.0%
TGAGAATGCAGAGGGAAGAA+GGG - chr8_4:16683518-16683537 None:intergenic 45.0%
TTGAGAATGCAGAGGGAAGA+AGG - chr8_4:16683519-16683538 None:intergenic 45.0%
! GTGGAAAGCGTGTTTACCTT+CGG - chr8_4:16683602-16683621 None:intergenic 45.0%
! TTGAACCAGAGACTTTGAGG+AGG + chr8_4:16684153-16684172 Msa1316390:intron 45.0%
GTACTAGTCCTAACCCACTT+GGG - chr8_4:16684218-16684237 None:intergenic 45.0%
AGTACTAGTCCTAACCCACT+TGG - chr8_4:16684219-16684238 None:intergenic 45.0%
! AGGAGAGACAGCGTTAGTTT+AGG + chr8_4:16684269-16684288 Msa1316390:intron 45.0%
! GGAGAGACAGCGTTAGTTTA+GGG + chr8_4:16684270-16684289 Msa1316390:intron 45.0%
!!! TCGGGTCATGTCATTTTCTG+TGG + chr8_4:16684541-16684560 Msa1316390:CDS 45.0%
!!! GTCATGTCATTTTCTGTGGC+TGG + chr8_4:16684545-16684564 Msa1316390:CDS 45.0%
AGTGCTATGGGTGCTTCATT+TGG + chr8_4:16684771-16684790 Msa1316390:CDS 45.0%
TGAGAATGCAGAGGGAAGAA+GGG - chr8_4:16683518-16683537 None:intergenic 45.0%
TTGAGAATGCAGAGGGAAGA+AGG - chr8_4:16683519-16683538 None:intergenic 45.0%
! GTGGAAAGCGTGTTTACCTT+CGG - chr8_4:16683602-16683621 None:intergenic 45.0%
! TTGAACCAGAGACTTTGAGG+AGG + chr8_4:16684153-16684172 Msa1316390:intron 45.0%
GTACTAGTCCTAACCCACTT+GGG - chr8_4:16684218-16684237 None:intergenic 45.0%
AGTACTAGTCCTAACCCACT+TGG - chr8_4:16684219-16684238 None:intergenic 45.0%
! AGGAGAGACAGCGTTAGTTT+AGG + chr8_4:16684269-16684288 Msa1316390:intron 45.0%
! GGAGAGACAGCGTTAGTTTA+GGG + chr8_4:16684270-16684289 Msa1316390:intron 45.0%
!!! TCGGGTCATGTCATTTTCTG+TGG + chr8_4:16684541-16684560 Msa1316390:CDS 45.0%
!!! GTCATGTCATTTTCTGTGGC+TGG + chr8_4:16684545-16684564 Msa1316390:CDS 45.0%
AGTGCTATGGGTGCTTCATT+TGG + chr8_4:16684771-16684790 Msa1316390:CDS 45.0%
!! GGGTTTTGGTGTATGCCCTT+TGG + chr8_4:16683481-16683500 Msa1316390:five_prime_UTR 50.0%
!! GTGGAATTGGCTTGGGACTT+GGG - chr8_4:16684187-16684206 None:intergenic 50.0%
!! AGTGGAATTGGCTTGGGACT+TGG - chr8_4:16684188-16684207 None:intergenic 50.0%
AGTCCCAAGCCAATTCCACT+AGG + chr8_4:16684188-16684207 Msa1316390:intron 50.0%
! TGGCCTAGTGGAATTGGCTT+GGG - chr8_4:16684194-16684213 None:intergenic 50.0%
TTGGCCTAGTGGAATTGGCT+TGG - chr8_4:16684195-16684214 None:intergenic 50.0%
!! TTGGGTTGGCCTAGTGGAAT+TGG - chr8_4:16684200-16684219 None:intergenic 50.0%
TAGTCCTAACCCACTTGGGT+TGG - chr8_4:16684214-16684233 None:intergenic 50.0%
AGGCAACGAGGAGGTAATTG+GGG - chr8_4:16684506-16684525 None:intergenic 50.0%
GAGGCAACGAGGAGGTAATT+GGG - chr8_4:16684507-16684526 None:intergenic 50.0%
TGGGCATACCTTTCCACACT+GGG - chr8_4:16684596-16684615 None:intergenic 50.0%
TTGGGCATACCTTTCCACAC+TGG - chr8_4:16684597-16684616 None:intergenic 50.0%
!! GGGTTTTGGTGTATGCCCTT+TGG + chr8_4:16683481-16683500 Msa1316390:five_prime_UTR 50.0%
!! GTGGAATTGGCTTGGGACTT+GGG - chr8_4:16684187-16684206 None:intergenic 50.0%
!! AGTGGAATTGGCTTGGGACT+TGG - chr8_4:16684188-16684207 None:intergenic 50.0%
AGTCCCAAGCCAATTCCACT+AGG + chr8_4:16684188-16684207 Msa1316390:intron 50.0%
! TGGCCTAGTGGAATTGGCTT+GGG - chr8_4:16684194-16684213 None:intergenic 50.0%
TTGGCCTAGTGGAATTGGCT+TGG - chr8_4:16684195-16684214 None:intergenic 50.0%
!! TTGGGTTGGCCTAGTGGAAT+TGG - chr8_4:16684200-16684219 None:intergenic 50.0%
TAGTCCTAACCCACTTGGGT+TGG - chr8_4:16684214-16684233 None:intergenic 50.0%
AGGCAACGAGGAGGTAATTG+GGG - chr8_4:16684506-16684525 None:intergenic 50.0%
GAGGCAACGAGGAGGTAATT+GGG - chr8_4:16684507-16684526 None:intergenic 50.0%
TGGGCATACCTTTCCACACT+GGG - chr8_4:16684596-16684615 None:intergenic 50.0%
TTGGGCATACCTTTCCACAC+TGG - chr8_4:16684597-16684616 None:intergenic 50.0%
GTGCTCCTCCTCAAAGTCTC+TGG - chr8_4:16684161-16684180 None:intergenic 55.0%
TTCCACTAGGCCAACCCAAG+TGG + chr8_4:16684201-16684220 Msa1316390:intron 55.0%
TCCACTAGGCCAACCCAAGT+GGG + chr8_4:16684202-16684221 Msa1316390:intron 55.0%
! TAGGCCAACCCAAGTGGGTT+AGG + chr8_4:16684207-16684226 Msa1316390:intron 55.0%
GGCAACGAGGAGGTAATTGG+GGG - chr8_4:16684505-16684524 None:intergenic 55.0%
CGAGGCAACGAGGAGGTAAT+TGG - chr8_4:16684508-16684527 None:intergenic 55.0%
AAGGACAGCGTTTCCCAGTG+TGG + chr8_4:16684580-16684599 Msa1316390:CDS 55.0%
CAGCGTTTCCCAGTGTGGAA+AGG + chr8_4:16684585-16684604 Msa1316390:CDS 55.0%
GTGCTCCTCCTCAAAGTCTC+TGG - chr8_4:16684161-16684180 None:intergenic 55.0%
TTCCACTAGGCCAACCCAAG+TGG + chr8_4:16684201-16684220 Msa1316390:intron 55.0%
TCCACTAGGCCAACCCAAGT+GGG + chr8_4:16684202-16684221 Msa1316390:intron 55.0%
! TAGGCCAACCCAAGTGGGTT+AGG + chr8_4:16684207-16684226 Msa1316390:intron 55.0%
GGCAACGAGGAGGTAATTGG+GGG - chr8_4:16684505-16684524 None:intergenic 55.0%
CGAGGCAACGAGGAGGTAAT+TGG - chr8_4:16684508-16684527 None:intergenic 55.0%
AAGGACAGCGTTTCCCAGTG+TGG + chr8_4:16684580-16684599 Msa1316390:CDS 55.0%
CAGCGTTTCCCAGTGTGGAA+AGG + chr8_4:16684585-16684604 Msa1316390:CDS 55.0%
!! ACCCACTTGGGTTGGCCTAG+TGG - chr8_4:16684206-16684225 None:intergenic 60.0%
CGTGATTCGAGGCAACGAGG+AGG - chr8_4:16684515-16684534 None:intergenic 60.0%
GCCTCGAATCACGCCGTGTT+CGG + chr8_4:16684522-16684541 Msa1316390:CDS 60.0%
CCTCGAATCACGCCGTGTTC+GGG + chr8_4:16684523-16684542 Msa1316390:CDS 60.0%
!! ACCCACTTGGGTTGGCCTAG+TGG - chr8_4:16684206-16684225 None:intergenic 60.0%
CGTGATTCGAGGCAACGAGG+AGG - chr8_4:16684515-16684534 None:intergenic 60.0%
GCCTCGAATCACGCCGTGTT+CGG + chr8_4:16684522-16684541 Msa1316390:CDS 60.0%
CCTCGAATCACGCCGTGTTC+GGG + chr8_4:16684523-16684542 Msa1316390:CDS 60.0%
CGGCGTGATTCGAGGCAACG+AGG - chr8_4:16684518-16684537 None:intergenic 65.0%
CCCGAACACGGCGTGATTCG+AGG - chr8_4:16684526-16684545 None:intergenic 65.0%
CGGCGTGATTCGAGGCAACG+AGG - chr8_4:16684518-16684537 None:intergenic 65.0%
CCCGAACACGGCGTGATTCG+AGG - chr8_4:16684526-16684545 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 16683479 16684824 16683479 ID=Msa1316390;Name=Msa1316390
chr8_4 mRNA 16683479 16684824 16683479 ID=Msa1316390-mRNA-1;Parent=Msa1316390;Name=Msa1316390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=200|1|0.66|1|0.5|0.33|3|0|85
chr8_4 exon 16683479 16683604 16683479 ID=Msa1316390-mRNA-1:exon:6207;Parent=Msa1316390-mRNA-1
chr8_4 exon 16684390 16684606 16684390 ID=Msa1316390-mRNA-1:exon:6208;Parent=Msa1316390-mRNA-1
chr8_4 exon 16684713 16684824 16684713 ID=Msa1316390-mRNA-1:exon:6209;Parent=Msa1316390-mRNA-1
chr8_4 five_prime_UTR 16683479 16683604 16683479 ID=Msa1316390-mRNA-1:five_prime_utr;Parent=Msa1316390-mRNA-1
chr8_4 five_prime_UTR 16684390 16684463 16684390 ID=Msa1316390-mRNA-1:five_prime_utr;Parent=Msa1316390-mRNA-1
chr8_4 CDS 16684464 16684606 16684464 ID=Msa1316390-mRNA-1:cds;Parent=Msa1316390-mRNA-1
chr8_4 CDS 16684713 16684824 16684713 ID=Msa1316390-mRNA-1:cds;Parent=Msa1316390-mRNA-1
Gene Sequence

>Msa1316390

ATGATTGTATCTGGGTTGAGTGGTAGTCTCTCAAGATGCCCCCAATTACCTCCTCGTTGCCTCGAATCACGCCGTGTTCGGGTCATGTCATTTTCTGTGGCTGGTAAATGTATACAAAGGACAGCGTTTCCCAGTGTGGAAAGAACCAGTTTTTCATCTCCTAGATTAAAATGTGATAGACGACAAGTTCAATTGGTCAGAAGTGCTATGGGTGCTTCATTTGGTGATATGTCAAATGATTCTGCTGGTAACTTT
Protein sequence

>Msa1316390

MIVSGLSGSLSRCPQLPPRCLESRRVRVMSFSVAGKCIQRTAFPSVERTSFSSPRLKCDRRQVQLVRSAMGASFGDMSNDSAGNF