AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1317150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1317150 MtrunA17_Chr8g0347321 94.582 443 21 2 6 446 1 442 0.0 871
Msa1317150 MtrunA17_Chr8g0387921 60.140 429 154 3 19 446 10 422 0.0 542
Msa1317150 MtrunA17_Chr6g0456691 43.250 400 218 4 48 443 25 419 2.78e-118 352
Msa1317150 MtrunA17_Chr5g0414061 43.056 360 193 7 90 443 85 438 1.77e-103 315
Msa1317150 MtrunA17_Chr5g0414041 46.176 353 176 7 99 443 87 433 1.47e-102 312
Msa1317150 MtrunA17_Chr5g0414091 41.880 351 194 5 99 443 85 431 3.85e-100 306
Msa1317150 MtrunA17_Chr7g0226991 40.166 361 203 6 90 443 48 402 1.99e-97 298
Msa1317150 MtrunA17_Chr3g0140371 40.456 351 195 6 99 443 18 360 6.82e-97 295
Msa1317150 MtrunA17_Chr5g0414051 39.231 390 219 9 62 443 41 420 3.60e-96 296
Msa1317150 MtrunA17_Chr8g0391481 38.482 382 221 7 72 446 1 375 4.79e-95 291
Msa1317150 MtrunA17_Chr6g0456681 40.162 371 207 6 83 443 43 408 3.90e-91 282
Msa1317150 MtrunA17_Chr6g0451321 38.571 350 202 5 99 443 116 457 1.05e-90 283
Msa1317150 MtrunA17_Chr2g0283851 38.611 360 192 11 100 444 56 401 2.77e-84 264
Msa1317150 MtrunA17_Chr1g0188191 35.969 392 233 10 61 443 199 581 2.88e-78 254
Msa1317150 MtrunA17_Chr6g0476041 35.457 361 220 8 91 443 159 514 6.84e-74 241
Msa1317150 MtrunA17_Chr7g0221281 39.749 239 137 4 207 443 3 236 8.48e-64 206
Msa1317150 MtrunA17_Chr7g0259711 33.151 365 203 14 99 444 91 433 9.61e-52 180
Msa1317150 MtrunA17_Chr7g0258851 31.476 359 217 10 98 444 247 588 1.80e-51 183
Msa1317150 MtrunA17_Chr1g0175671 31.421 366 208 10 98 444 169 510 5.52e-51 180
Msa1317150 MtrunA17_Chr2g0287901 30.252 357 223 10 99 444 97 438 1.89e-49 174
Msa1317150 MtrunA17_Chr8g0368461 28.325 406 247 11 64 444 143 529 3.55e-49 176
Msa1317150 MtrunA17_Chr4g0071391 30.137 365 212 11 98 444 108 447 5.71e-48 171
Msa1317150 MtrunA17_Chr5g0413271 28.052 385 241 11 65 444 124 477 6.40e-47 168
Msa1317150 MtrunA17_Chr7g0269991 31.768 362 203 13 99 445 137 469 1.22e-46 167
Msa1317150 MtrunA17_Chr5g0414081 39.698 199 112 4 252 443 2 199 1.89e-46 159
Msa1317150 MtrunA17_Chr5g0430371 31.967 366 220 11 88 443 53 399 1.98e-46 165
Msa1317150 MtrunA17_Chr8g0360521 30.579 363 224 13 92 443 64 409 3.02e-45 162
Msa1317150 MtrunA17_Chr8g0384151 30.295 373 214 13 85 443 76 416 4.39e-45 162
Msa1317150 MtrunA17_Chr7g0221271 51.562 128 62 0 98 225 78 205 1.35e-44 154
Msa1317150 MtrunA17_Chr1g0213281 31.165 369 222 14 90 444 166 516 2.18e-44 162
Msa1317150 MtrunA17_Chr3g0105301 31.250 352 217 12 99 443 43 376 1.96e-43 156
Msa1317150 MtrunA17_Chr3g0113671 27.731 357 237 10 99 445 106 451 3.53e-43 157
Msa1317150 MtrunA17_Chr1g0171171 28.533 368 230 11 81 443 36 375 7.65e-43 155
Msa1317150 MtrunA17_Chr7g0243301 28.647 377 237 14 86 445 50 411 8.65e-42 153
Msa1317150 MtrunA17_Chr4g0005491 28.756 386 240 15 72 439 35 403 1.18e-41 152
Msa1317150 MtrunA17_Chr4g0068111 30.556 360 221 12 99 446 114 456 1.45e-41 153
Msa1317150 MtrunA17_Chr8g0384421 27.962 422 238 13 60 443 79 472 1.43e-39 148
Msa1317150 MtrunA17_Chr3g0114101 29.086 361 221 13 99 443 89 430 1.54e-39 147
Msa1317150 MtrunA17_Chr3g0091031 28.889 360 210 13 96 445 27 350 3.45e-39 144
Msa1317150 MtrunA17_Chr5g0437601 28.571 357 228 12 99 443 83 424 5.33e-39 145
Msa1317150 MtrunA17_Chr3g0085081 28.611 360 211 13 96 445 27 350 1.66e-38 142
Msa1317150 MtrunA17_Chr4g0006991 27.095 358 234 10 98 443 85 427 6.17e-38 142
Msa1317150 MtrunA17_Chr4g0005481 27.654 358 227 10 99 443 108 446 7.80e-38 143
Msa1317150 MtrunA17_Chr2g0283581 29.834 362 211 13 93 443 29 358 4.24e-37 139
Msa1317150 MtrunA17_Chr3g0085071 27.616 344 218 8 98 439 45 359 6.77e-37 138
Msa1317150 MtrunA17_Chr1g0210771 27.344 384 231 16 73 443 34 382 5.38e-36 136
Msa1317150 MtrunA17_Chr6g0455901 27.297 381 231 14 85 443 60 416 2.24e-35 135
Msa1317150 MtrunA17_Chr7g0221291 55.319 94 42 0 98 191 78 171 3.45e-35 128
Msa1317150 MtrunA17_Chr3g0097891 27.614 373 219 11 85 444 17 351 5.38e-35 133
Msa1317150 MtrunA17_Chr2g0325481 27.624 362 214 10 92 443 43 366 1.53e-34 132
Msa1317150 MtrunA17_Chr7g0242701 25.698 358 239 11 98 443 85 427 2.48e-34 132
Msa1317150 MtrunA17_Chr3g0126021 27.764 407 238 15 60 444 88 460 3.00e-34 133
Msa1317150 MtrunA17_Chr2g0314801 27.684 354 216 11 98 443 32 353 9.90e-34 129
Msa1317150 MtrunA17_Chr4g0070341 27.027 407 242 15 63 443 94 471 2.01e-33 131
Msa1317150 MtrunA17_Chr4g0045941 26.923 364 203 12 99 444 33 351 7.01e-33 127
Msa1317150 MtrunA17_Chr4g0049421 25.479 365 238 11 99 446 48 395 2.63e-32 127
Msa1317150 MtrunA17_Chr5g0437291 25.762 361 242 13 99 445 165 513 2.80e-32 128
Msa1317150 MtrunA17_Chr5g0413851 28.532 361 216 14 92 444 34 360 4.46e-32 125
Msa1317150 MtrunA17_Chr4g0045951 27.005 374 209 13 89 444 36 363 1.82e-31 124
Msa1317150 MtrunA17_Chr4g0041011 26.415 371 216 14 85 443 38 363 8.10e-29 116
Msa1317150 MtrunA17_Chr2g0314791 26.648 364 213 12 94 444 24 346 5.67e-28 113
Msa1317150 MtrunA17_Chr7g0275501 68.056 72 18 2 371 442 2 68 4.98e-26 100
Msa1317150 MtrunA17_Chr1g0178081 31.707 205 127 6 87 281 43 244 6.70e-23 99.4
Msa1317150 MtrunA17_Chr1g0209921 45.361 97 51 2 98 192 43 139 6.28e-20 89.4
Msa1317150 MtrunA17_Chr2g0287911 45.763 59 25 1 96 154 58 109 2.53e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1317150 AT1G01430.1 54.767 451 191 7 1 445 6 449 2.29e-179 509
Msa1317150 AT4G01080.1 56.512 430 145 8 18 445 43 432 5.46e-171 487
Msa1317150 AT4G11090.1 50.117 429 199 5 20 446 13 428 7.06e-160 459
Msa1317150 AT4G23790.1 51.370 438 196 7 13 446 6 430 1.04e-159 458
Msa1317150 AT5G15890.1 41.262 412 202 7 56 443 127 522 5.14e-112 340
Msa1317150 AT1G70230.1 43.642 346 181 6 99 443 79 411 2.27e-106 322
Msa1317150 AT3G02440.1 43.305 351 188 5 99 445 127 470 2.12e-103 316
Msa1317150 AT3G02440.2 43.305 351 188 5 99 445 135 478 2.14e-103 316
Msa1317150 AT5G15900.1 41.379 348 196 4 99 443 67 409 4.46e-100 306
Msa1317150 AT5G15900.2 41.379 348 196 4 99 443 86 428 5.32e-100 306
Msa1317150 AT3G28150.1 39.943 353 200 4 95 443 66 410 8.42e-99 302
Msa1317150 AT5G51640.1 35.537 363 220 8 91 443 130 488 3.67e-73 238
Msa1317150 AT4G25360.1 35.655 359 218 7 94 443 170 524 6.44e-72 236
Msa1317150 AT4G25360.2 35.655 359 218 7 94 443 170 524 6.44e-72 236
Msa1317150 AT5G19160.1 32.208 385 223 14 81 444 78 445 2.81e-53 185
Msa1317150 AT3G12060.1 31.148 366 210 11 99 445 196 538 3.57e-51 181
Msa1317150 AT3G06080.2 31.564 358 219 12 99 444 103 446 4.28e-51 179
Msa1317150 AT5G06700.1 31.319 364 209 11 99 444 255 595 1.58e-49 178
Msa1317150 AT1G60790.1 29.201 363 225 11 99 446 190 535 2.05e-47 171
Msa1317150 AT5G49340.1 29.442 394 237 10 69 444 72 442 3.37e-47 169
Msa1317150 AT5G20590.1 32.033 359 212 13 99 444 141 480 4.88e-47 169
Msa1317150 AT3G11570.1 31.503 346 204 11 112 444 95 420 3.09e-46 166
Msa1317150 AT5G64020.1 30.811 370 213 11 99 443 57 408 8.41e-46 164
Msa1317150 AT1G73140.1 29.315 365 232 10 92 446 60 408 5.13e-45 162
Msa1317150 AT5G06230.2 29.396 381 224 13 87 445 9 366 5.55e-45 160
Msa1317150 AT1G48880.1 30.194 361 202 12 98 443 114 439 1.06e-44 162
Msa1317150 AT5G06230.1 29.396 381 224 13 87 445 50 407 1.47e-44 160
Msa1317150 AT2G30010.1 28.771 358 227 8 99 444 56 397 2.36e-44 160
Msa1317150 AT3G62390.1 30.387 362 213 13 98 445 137 473 3.76e-44 161
Msa1317150 AT2G30010.2 29.526 359 223 10 99 444 99 440 5.19e-44 160
Msa1317150 AT5G58600.1 29.692 357 219 12 99 444 66 401 1.05e-41 152
Msa1317150 AT3G11030.1 29.213 356 228 12 99 443 106 448 4.85e-40 149
Msa1317150 AT5G64470.2 29.640 361 225 14 99 444 55 401 1.17e-39 147
Msa1317150 AT2G40150.1 25.895 363 244 10 98 445 70 422 1.19e-39 147
Msa1317150 AT5G64470.3 29.330 358 224 13 99 444 55 395 1.67e-39 147
Msa1317150 AT2G38320.1 29.282 362 229 14 98 445 58 406 3.71e-39 146
Msa1317150 AT2G30900.1 29.526 359 209 12 99 446 43 368 5.67e-39 144
Msa1317150 AT2G40320.1 25.706 354 242 9 99 443 81 422 2.87e-38 144
Msa1317150 AT2G40160.1 26.209 393 249 11 77 439 38 419 4.42e-38 143
Msa1317150 AT2G40160.2 25.686 401 253 12 77 445 38 425 4.52e-38 144
Msa1317150 AT3G55990.1 26.582 395 264 12 61 445 107 485 5.07e-38 144
Msa1317150 AT2G14530.1 28.065 367 240 10 89 443 52 406 5.35e-38 143
Msa1317150 AT5G01360.2 26.611 357 238 11 98 443 35 378 2.87e-37 140
Msa1317150 AT5G01360.1 26.611 357 238 11 98 443 91 434 8.37e-37 140
Msa1317150 AT5G19160.2 31.716 268 159 9 81 333 78 336 1.50e-36 137
Msa1317150 AT5G20680.6 27.177 379 236 11 83 443 101 457 1.94e-36 139
Msa1317150 AT5G20680.4 27.177 379 236 11 83 443 195 551 3.61e-36 140
Msa1317150 AT5G20680.1 27.177 379 236 11 83 443 195 551 3.61e-36 140
Msa1317150 AT5G20680.3 27.177 379 236 11 83 443 195 551 3.61e-36 140
Msa1317150 AT5G20680.5 27.177 379 236 11 83 443 195 551 3.61e-36 140
Msa1317150 AT5G20680.2 27.177 379 236 11 83 443 177 533 3.71e-36 140
Msa1317150 AT3G54260.1 29.330 358 210 15 99 445 51 376 2.75e-35 135
Msa1317150 AT3G06080.1 31.746 252 153 7 99 339 103 346 2.86e-35 134
Msa1317150 AT2G42570.1 27.717 368 222 10 88 446 35 367 3.58e-34 131
Msa1317150 AT5G01620.3 26.554 354 239 9 98 443 117 457 3.97e-34 133
Msa1317150 AT5G01620.2 26.554 354 239 9 98 443 109 449 4.33e-34 133
Msa1317150 AT5G01620.1 26.554 354 239 9 98 443 109 449 4.33e-34 133
Msa1317150 AT1G29050.1 27.322 366 226 10 89 444 44 379 5.88e-33 128
Msa1317150 AT2G38320.2 28.788 330 209 13 129 445 7 323 1.75e-32 126
Msa1317150 AT2G37720.1 27.446 368 216 12 99 443 143 482 2.81e-32 128
Msa1317150 AT1G78710.1 26.455 378 214 16 86 445 27 358 4.19e-32 125
Msa1317150 AT3G62390.2 30.651 261 158 8 98 346 137 386 2.17e-31 125
Msa1317150 AT2G34070.1 26.934 349 221 9 99 443 65 383 4.15e-31 123
Msa1317150 AT3G14850.2 27.119 354 218 11 99 444 34 355 8.93e-31 122
Msa1317150 AT2G31110.2 26.842 380 229 12 77 445 23 364 1.18e-30 122
Msa1317150 AT1G78710.2 26.963 382 172 17 78 445 10 298 2.22e-28 114
Msa1317150 AT5G58600.2 29.832 238 148 8 99 331 66 289 4.99e-28 113
Msa1317150 AT5G06230.3 28.358 268 165 9 87 336 50 308 9.19e-27 110
Msa1317150 AT2G40160.3 33.136 169 95 3 77 227 38 206 1.15e-26 109
Msa1317150 AT5G20590.2 27.935 247 157 8 99 333 141 378 3.49e-26 109
Msa1317150 AT5G64470.4 27.985 268 174 10 99 354 55 315 1.25e-25 107
Msa1317150 AT5G64470.1 28.090 267 173 10 99 353 55 314 3.36e-25 105
Msa1317150 AT2G30900.2 32.124 193 113 6 99 282 43 226 3.60e-24 102
Msa1317150 AT2G31110.3 31.111 180 119 1 77 251 23 202 4.18e-23 98.6

Find 109 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATACTCTTGACCATAAT+TGG 0.140160 8_4:-18230836 Msa1317150:CDS
CTGATTTAGGGTACCTTTAT+TGG 0.167850 8_4:-18232863 Msa1317150:CDS
ATGAGACTAAGAAACTTCTT+AGG 0.206896 8_4:+18232801 None:intergenic
AATAAAGCAATGGCCTTTAT+TGG 0.211094 8_4:-18232774 Msa1317150:CDS
ATAATTACCGTTTCGAATTC+AGG 0.230063 8_4:-18230198 Msa1317150:three_prime_UTR
TTTGGTAATTCACACTCTTT+TGG 0.238518 8_4:+18232831 None:intergenic
ACTTGAAATTCATCATATTC+TGG 0.258772 8_4:+18233159 None:intergenic
TTGTTTCTGTTTCCACCTTT+TGG 0.262438 8_4:-18233428 Msa1317150:five_prime_UTR
TAAACAAAACAGTTGCATTA+TGG 0.267888 8_4:+18230619 None:intergenic
CATAATCAAAATTGTTGAAT+TGG 0.296433 8_4:+18230808 None:intergenic
TGAGCATAAACTAAATAAAT+AGG 0.297454 8_4:+18229964 None:intergenic
CATGAAAACAACACAGTATC+TGG 0.304134 8_4:-18230741 Msa1317150:CDS
GGAAAGAATTTGACTGATCT+AGG 0.322511 8_4:-18230702 Msa1317150:CDS
ACTTCACACTCTCAGTAATA+TGG 0.322961 8_4:-18230932 Msa1317150:CDS
TTGTGATTGGTGGTGGAAAA+TGG 0.329782 8_4:-18230785 Msa1317150:CDS
GTGGAAACAGAAACAAGTTT+TGG 0.331062 8_4:+18233435 None:intergenic
TGGTGTGAACTTGAAACTTT+TGG 0.331395 8_4:-18230427 Msa1317150:CDS
TTGCAAATGGCTGGAATTGC+CGG 0.332752 8_4:+18230355 None:intergenic
TTGGACTCCACCTCCAATAA+AGG 0.334944 8_4:+18232850 None:intergenic
TGAATTGGTTGGTCCAATTA+TGG 0.357146 8_4:+18230823 None:intergenic
AGATCAGTCAAATTCTTTCC+AGG 0.366670 8_4:+18230705 None:intergenic
TCATATTCTGGTGCTTTAAG+AGG 0.390318 8_4:+18233171 None:intergenic
GAATGGAGTTTCATCTTCAA+TGG 0.394596 8_4:-18230871 Msa1317150:CDS
TTTGTCTCTGTTTGAGGAGG+AGG 0.395992 8_4:+18233219 None:intergenic
ATGGGCATCCAGGACCTTAC+CGG 0.398094 8_4:-18230374 Msa1317150:CDS
TGAGATGGAATCACCAATAA+AGG 0.402777 8_4:+18232761 None:intergenic
AGGAATATAGATCAAAGATA+TGG 0.402990 8_4:-18230971 Msa1317150:CDS
AAAATGGAAGACCTGATTTA+GGG 0.405923 8_4:-18232875 Msa1317150:CDS
ATCAAAATTGTTGAATTGGT+TGG 0.411356 8_4:+18230812 None:intergenic
TACCAGGACCAATTGATTCA+TGG 0.412261 8_4:-18230287 Msa1317150:CDS
ATAGCTGCTTTAATAAGAAA+AGG 0.412745 8_4:+18230906 None:intergenic
ATCGATTTCACCCTCTTTGA+AGG 0.413565 8_4:+18230527 None:intergenic
TCAAGTGTAGTAATTAGTAT+AGG 0.414250 8_4:+18233508 None:intergenic
TCTTATCCTTTGCAAATGGC+TGG 0.416549 8_4:+18230346 None:intergenic
CAAGTGTAGTAATTAGTATA+GGG 0.423440 8_4:+18233509 None:intergenic
TTTGATTATGTTGTGATTGG+TGG 0.423731 8_4:-18230795 Msa1317150:CDS
TAGCTGCTTTAATAAGAAAA+GGG 0.432646 8_4:+18230907 None:intergenic
AAATCCAATCTCCCACAAAA+AGG 0.432762 8_4:+18232967 None:intergenic
ACAAACTGATTACGTGTTGC+CGG 0.433245 8_4:+18230082 None:intergenic
TAAGGTCCTGGATGCCCATC+TGG 0.437991 8_4:+18230378 None:intergenic
CTTAGACCAGATGGGCATCC+AGG 0.439003 8_4:-18230384 Msa1317150:CDS
ATTTAGGGTACCTTTATTGG+AGG 0.441718 8_4:-18232860 Msa1317150:CDS
CAATAAAGGTACCCTAAATC+AGG 0.442542 8_4:+18232864 None:intergenic
GTCTCTGTTTGAGGAGGAGG+AGG 0.445712 8_4:+18233222 None:intergenic
CTACACTTGACAACACCGTT+TGG 0.447905 8_4:-18233495 Msa1317150:five_prime_UTR
ACAAGCTGCATGAATAAAAC+AGG 0.449222 8_4:-18233563 None:intergenic
GAGAATGGTGAATGGTTTAG+TGG 0.451015 8_4:-18230573 Msa1317150:CDS
TCATTCTTATCCTTTGCAAA+TGG 0.454326 8_4:+18230342 None:intergenic
TAGTAATTAGTATAGGGAAA+TGG 0.454422 8_4:+18233515 None:intergenic
TAGGGTACCTTTATTGGAGG+TGG 0.459997 8_4:-18232857 Msa1317150:CDS
ATATCATTCCATGAATCAAT+TGG 0.466957 8_4:+18230279 None:intergenic
CTTTCATTGCTTAGACCAGA+TGG 0.467322 8_4:-18230393 Msa1317150:CDS
CTGCAAATTTAACACAGAAA+AGG 0.469651 8_4:+18233334 None:intergenic
TGTTTACATTGGTGTTTACC+AGG 0.471207 8_4:-18230303 Msa1317150:CDS
CACCATCTTGAGTGACCAAA+AGG 0.473479 8_4:+18233413 None:intergenic
CCTGTGAGAGGATGCAAAGT+AGG 0.476743 8_4:+18232721 None:intergenic
AACTTCTTAGGGTTGAACTT+TGG 0.488278 8_4:+18232813 None:intergenic
TCTGGCTGTCATTACTGTCC+TGG 0.491314 8_4:-18230723 Msa1317150:CDS
CTGAACATGGTTGCGTGAGA+TGG 0.497150 8_4:+18232746 None:intergenic
GATTATGTTGTGATTGGTGG+TGG 0.497535 8_4:-18230792 Msa1317150:CDS
ATGGTGAATGGTTTAGTGGA+GGG 0.498774 8_4:-18230569 Msa1317150:CDS
CAACTCTCATTTGTGTACAT+TGG 0.501989 8_4:+18232930 None:intergenic
TCACCATTCTCAAAGTGATC+TGG 0.502058 8_4:+18230585 None:intergenic
TGAGACTAAGAAACTTCTTA+GGG 0.503686 8_4:+18232802 None:intergenic
CATATTCTGGTGCTTTAAGA+GGG 0.506797 8_4:+18233172 None:intergenic
ATCACTTTGAGAATGGTGAA+TGG 0.515741 8_4:-18230581 Msa1317150:CDS
GAAAGAATTTGACTGATCTA+GGG 0.517914 8_4:-18230701 Msa1317150:CDS
TTTGCGAAGGCTTCGTCTCA+AGG 0.517932 8_4:-18230459 Msa1317150:CDS
TTGTAGTCATTATGTAAAAG+TGG 0.521403 8_4:-18233471 Msa1317150:five_prime_UTR
AAAACTTGCAAAAGCAAATG+AGG 0.521942 8_4:-18230121 Msa1317150:three_prime_UTR
GCTATTTATGAAGACATGAA+TGG 0.523460 8_4:-18230888 Msa1317150:CDS
CATAATGACTACAAACCAAA+CGG 0.523892 8_4:+18233480 None:intergenic
AATGGCTGGAATTGCCGGTA+AGG 0.526974 8_4:+18230360 None:intergenic
GTTGAGCTGCATTGTGTACA+TGG 0.528397 8_4:-18230161 Msa1317150:three_prime_UTR
TTCCATGAATCAATTGGTCC+TGG 0.529258 8_4:+18230285 None:intergenic
TTGATCTATATTCCTTGTCA+TGG 0.529841 8_4:+18230979 None:intergenic
TGGAATTGCCGGTAAGGTCC+TGG 0.531200 8_4:+18230366 None:intergenic
TTCAGAATGATTGTTTACAT+TGG 0.536286 8_4:-18230314 Msa1317150:CDS
TCGATTTCACCCTCTTTGAA+GGG 0.541605 8_4:+18230528 None:intergenic
TATAGGGAAATGGAAACCAT+TGG 0.545401 8_4:+18233525 None:intergenic
TCGTCTCAAGGATCTGAGAA+TGG 0.549295 8_4:-18230447 Msa1317150:CDS
GAGAGTGTGAAGTTATGAGA+GGG 0.550677 8_4:+18230942 None:intergenic
ACACCAGATCACTTTGAGAA+TGG 0.551530 8_4:-18230588 Msa1317150:CDS
ATGTGACAATAATATGTCCT+TGG 0.552768 8_4:-18230226 Msa1317150:three_prime_UTR
AGATGGTGAAGAGAGTACTA+AGG 0.555077 8_4:-18233398 Msa1317150:CDS
CTGTGAGAGGATGCAAAGTA+GGG 0.559761 8_4:+18232722 None:intergenic
TTTCATTGCTTAGACCAGAT+GGG 0.567059 8_4:-18230392 Msa1317150:CDS
TTCATGGAATGATATAGTAA+TGG 0.569254 8_4:-18230271 Msa1317150:CDS
TCGAACTGTACCCTTCAAAG+AGG 0.572283 8_4:-18230538 Msa1317150:CDS
TCATGGTAGACCTCAACAGC+TGG 0.573403 8_4:+18230996 None:intergenic
CCTACTTTGCATCCTCTCAC+AGG 0.574327 8_4:-18232721 Msa1317150:intron
AGAAGTTTCTTAGTCTCATG+AGG 0.579769 8_4:-18232797 Msa1317150:CDS
AGGTGACAACACAAATGAGC+CGG 0.583023 8_4:-18230101 Msa1317150:three_prime_UTR
TGAGAGTGTGAAGTTATGAG+AGG 0.587200 8_4:+18230941 None:intergenic
AACGGTAATTATTGTAACCA+AGG 0.591595 8_4:+18230209 None:intergenic
CGAACTGTACCCTTCAAAGA+GGG 0.598509 8_4:-18230537 Msa1317150:CDS
GCAATTCCAGCCATTTGCAA+AGG 0.599193 8_4:-18230352 Msa1317150:CDS
ACAGGTGGAACCAGCTGTTG+AGG 0.602644 8_4:-18231006 Msa1317150:intron
TGCAAAGTAGGGACTGAACA+TGG 0.605625 8_4:+18232733 None:intergenic
AGCTGCTTTAATAAGAAAAG+GGG 0.606112 8_4:+18230908 None:intergenic
CATCTTGAGTGACCAAAAGG+TGG 0.611853 8_4:+18233416 None:intergenic
AATTATGGTCAAGAGTATCA+AGG 0.611965 8_4:+18230838 None:intergenic
TCTCATGAGGAATAAAGCAA+TGG 0.629275 8_4:-18232784 Msa1317150:CDS
GATGTAGATTCTATCATGAG+AGG 0.632190 8_4:-18230498 Msa1317150:CDS
ATATTCTGGTGCTTTAAGAG+GGG 0.646371 8_4:+18233173 None:intergenic
TGAAGTTCAAGAGTTTGCGA+AGG 0.647288 8_4:-18230472 Msa1317150:CDS
AAGTTAACAATACCTGTGAG+AGG 0.670328 8_4:+18232709 None:intergenic
AATGGTGAATGGTTTAGTGG+AGG 0.679799 8_4:-18230570 Msa1317150:CDS
TGTTGAGGTCTACCATGACA+AGG 0.690643 8_4:-18230991 Msa1317150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTTAAAAATAAAAT+TGG - chr8_4:18232284-18232303 Msa1317150:intron 0.0%
!! TATTATATTTATAAAAATTA+TGG + chr8_4:18233489-18233508 None:intergenic 0.0%
!!! CTCATTTTAATTTAAAATTT+TGG + chr8_4:18231544-18231563 None:intergenic 10.0%
!!! CAAAATTTTAAATTAAAATG+AGG - chr8_4:18231542-18231561 Msa1317150:intron 10.0%
!! ATTTAATCATTCAAACAAAA+AGG + chr8_4:18230838-18230857 None:intergenic 15.0%
!!! TTATTTGAAAATTTTACTCT+TGG - chr8_4:18231076-18231095 Msa1317150:intron 15.0%
!!! GTTAACTTTTTCTTATAAAT+AGG + chr8_4:18231258-18231277 None:intergenic 15.0%
!! ATGTAAAATTGTTCAAAAAT+TGG - chr8_4:18231960-18231979 Msa1317150:intron 15.0%
!!! AATTGTTATATAGTTTTTGA+GGG + chr8_4:18232037-18232056 None:intergenic 15.0%
!!! TAATTGTTATATAGTTTTTG+AGG + chr8_4:18232038-18232057 None:intergenic 15.0%
!!! TGATTTAATACAAATTTTGT+AGG + chr8_4:18232435-18232454 None:intergenic 15.0%
!!! ATGGTAAGAAATTTTTTATG+TGG - chr8_4:18230372-18230391 Msa1317150:CDS 20.0%
!!! TTTTTCATACAATTTTGATG+AGG + chr8_4:18230591-18230610 None:intergenic 20.0%
!! CATCAAAATTGTATGAAAAA+TGG - chr8_4:18230591-18230610 Msa1317150:CDS 20.0%
!!! AAATTTTCAAATAAAACGCT+AGG + chr8_4:18231070-18231089 None:intergenic 20.0%
!! CATTTCATAAATGATGTATT+TGG + chr8_4:18231327-18231346 None:intergenic 20.0%
!! GTCAAATGTTTCTTATAAAT+AGG - chr8_4:18231357-18231376 Msa1317150:intron 20.0%
!!! ATATTCTGGTTTTTTAAACA+TGG - chr8_4:18231912-18231931 Msa1317150:intron 20.0%
!!! TAGAATTTAGAACATATTGT+AGG - chr8_4:18232056-18232075 Msa1317150:intron 20.0%
!!! AGAATTTAGAACATATTGTA+GGG - chr8_4:18232057-18232076 Msa1317150:intron 20.0%
!!! TTGTATTTGTTAGTTTTAGT+TGG + chr8_4:18232175-18232194 None:intergenic 20.0%
!! CATTGAAACAATAAATTAAC+TGG - chr8_4:18232252-18232271 Msa1317150:intron 20.0%
!! CATAATCAAAATTGTTGAAT+TGG + chr8_4:18232677-18232696 None:intergenic 20.0%
!!! AATTTTGATTATGTTGTGAT+TGG - chr8_4:18232684-18232703 Msa1317150:intron 20.0%
!! TGAGCATAAACTAAATAAAT+AGG + chr8_4:18233521-18233540 None:intergenic 20.0%
! TAGTAATTAGTATAGGGAAA+TGG + chr8_4:18229970-18229989 None:intergenic 25.0%
! CAAGTGTAGTAATTAGTATA+GGG + chr8_4:18229976-18229995 None:intergenic 25.0%
! TCAAGTGTAGTAATTAGTAT+AGG + chr8_4:18229977-18229996 None:intergenic 25.0%
! TTGTAGTCATTATGTAAAAG+TGG - chr8_4:18230011-18230030 Msa1317150:three_prime_UTR 25.0%
! ACTTGAAATTCATCATATTC+TGG + chr8_4:18230326-18230345 None:intergenic 25.0%
! ACAATTGTTTGTTTAGTTAC+AGG - chr8_4:18230483-18230502 Msa1317150:CDS 25.0%
!! TATTGTTAACTTTGTTGGTT+TGG - chr8_4:18230784-18230803 Msa1317150:CDS 25.0%
!! TAGAATATCAAGTTGCAAAT+TGG + chr8_4:18230813-18230832 None:intergenic 25.0%
! CTTGATATTGTGGATAAATA+CGG - chr8_4:18230918-18230937 Msa1317150:CDS 25.0%
! TGTTGTGATCTTTGATATTA+CGG - chr8_4:18231119-18231138 Msa1317150:intron 25.0%
!! TTTTTCTTATAAATAGGACC+GGG + chr8_4:18231252-18231271 None:intergenic 25.0%
!! CTTTTTCTTATAAATAGGAC+CGG + chr8_4:18231253-18231272 None:intergenic 25.0%
! ATGTTTCTTATAAATAGGAC+CGG - chr8_4:18231362-18231381 Msa1317150:intron 25.0%
!! TTTTACAAGCCAAAAAAAGT+TGG + chr8_4:18231449-18231468 None:intergenic 25.0%
! ATGTATTCAAATGTAGTAAC+AGG - chr8_4:18231511-18231530 Msa1317150:intron 25.0%
!!! GCATGTATGTCATTTTATAT+AGG - chr8_4:18231662-18231681 Msa1317150:intron 25.0%
!!! TAATTTGTTGACAATGACAA+GGG + chr8_4:18231693-18231712 None:intergenic 25.0%
!!! TTAATTTGTTGACAATGACA+AGG + chr8_4:18231694-18231713 None:intergenic 25.0%
! ATTTAGTAATGAACACAACT+TGG + chr8_4:18231876-18231895 None:intergenic 25.0%
! AGGAATATAGATCAAAGATA+TGG - chr8_4:18232511-18232530 Msa1317150:intron 25.0%
!! TAGCTGCTTTAATAAGAAAA+GGG + chr8_4:18232578-18232597 None:intergenic 25.0%
!! ATAGCTGCTTTAATAAGAAA+AGG + chr8_4:18232579-18232598 None:intergenic 25.0%
! ATCAAAATTGTTGAATTGGT+TGG + chr8_4:18232673-18232692 None:intergenic 25.0%
!! AAACAAGTTGTAATGCTTTT+CGG + chr8_4:18232821-18232840 None:intergenic 25.0%
! TAAACAAAACAGTTGCATTA+TGG + chr8_4:18232866-18232885 None:intergenic 25.0%
! TTCAGAATGATTGTTTACAT+TGG - chr8_4:18233168-18233187 Msa1317150:CDS 25.0%
! ATATCATTCCATGAATCAAT+TGG + chr8_4:18233206-18233225 None:intergenic 25.0%
! TTCATGGAATGATATAGTAA+TGG - chr8_4:18233211-18233230 Msa1317150:CDS 25.0%
! AAAAAATCCTGAATTCGAAA+CGG + chr8_4:18233294-18233313 None:intergenic 25.0%
!!! AATTCAGGATTTTTTGCTAT+AGG - chr8_4:18233299-18233318 Msa1317150:CDS 25.0%
!!! GTTGCTTTTATCATAACCAA+TGG - chr8_4:18229941-18229960 Msa1317150:three_prime_UTR 30.0%
CATAATGACTACAAACCAAA+CGG + chr8_4:18230005-18230024 None:intergenic 30.0%
CTGCAAATTTAACACAGAAA+AGG + chr8_4:18230151-18230170 None:intergenic 30.0%
ATAATGCTAAGTGTTTATGC+TGG - chr8_4:18230430-18230449 Msa1317150:CDS 30.0%
AAAAATGGAAGACCTGATTT+AGG - chr8_4:18230606-18230625 Msa1317150:CDS 30.0%
AAAATGGAAGACCTGATTTA+GGG - chr8_4:18230607-18230626 Msa1317150:CDS 30.0%
!! TTTGGTAATTCACACTCTTT+TGG + chr8_4:18230654-18230673 None:intergenic 30.0%
TGAGACTAAGAAACTTCTTA+GGG + chr8_4:18230683-18230702 None:intergenic 30.0%
ATGAGACTAAGAAACTTCTT+AGG + chr8_4:18230684-18230703 None:intergenic 30.0%
AATAAAGCAATGGCCTTTAT+TGG - chr8_4:18230708-18230727 Msa1317150:CDS 30.0%
! ACAGGTATTGTTAACTTTGT+TGG - chr8_4:18230779-18230798 Msa1317150:CDS 30.0%
TAATACGATCCTTGATATTG+TGG - chr8_4:18230908-18230927 Msa1317150:CDS 30.0%
CGTATTTATCCACAATATCA+AGG + chr8_4:18230920-18230939 None:intergenic 30.0%
TGTAAGTGTAGATATGATTG+TGG - chr8_4:18230994-18231013 Msa1317150:CDS 30.0%
GTTGTGATCTTTGATATTAC+GGG - chr8_4:18231120-18231139 Msa1317150:intron 30.0%
!! GTATGTTTTTAAATTGCGAG+CGG + chr8_4:18231225-18231244 None:intergenic 30.0%
! TTTTCTTATAAATAGGACCG+GGG + chr8_4:18231251-18231270 None:intergenic 30.0%
!!! TGTTTCACTCCAACTTTTTT+TGG - chr8_4:18231437-18231456 Msa1317150:intron 30.0%
!!! CAGCATGAAAACATCTTTTT+TGG - chr8_4:18231480-18231499 Msa1317150:intron 30.0%
GTTTACATCATAGAATCACA+TGG + chr8_4:18231568-18231587 None:intergenic 30.0%
TCATTGTAGGTAAACCTTTA+TGG + chr8_4:18231781-18231800 None:intergenic 30.0%
AGGTTTACCTACAATGAATA+TGG - chr8_4:18231784-18231803 Msa1317150:intron 30.0%
AAAAGCTTATCATGAACATC+TGG + chr8_4:18231822-18231841 None:intergenic 30.0%
AACATAGTTGTAAAGCAAAG+AGG + chr8_4:18232110-18232129 None:intergenic 30.0%
CAATACATGATGCAACAAAT+AGG + chr8_4:18232398-18232417 None:intergenic 30.0%
TCATGTATTGTCTCAAGAAT+TGG - chr8_4:18232408-18232427 Msa1317150:intron 30.0%
! TCCAATGATTTTACTCTAAC+AGG - chr8_4:18232458-18232477 Msa1317150:intron 30.0%
ACCTGTTAGAGTAAAATCAT+TGG + chr8_4:18232462-18232481 None:intergenic 30.0%
! AATGATTTTACTCTAACAGG+TGG - chr8_4:18232461-18232480 Msa1317150:intron 30.0%
TTGATCTATATTCCTTGTCA+TGG + chr8_4:18232506-18232525 None:intergenic 30.0%
! AGCTGCTTTAATAAGAAAAG+GGG + chr8_4:18232577-18232596 None:intergenic 30.0%
GCTATTTATGAAGACATGAA+TGG - chr8_4:18232594-18232613 Msa1317150:intron 30.0%
!! AATTATGGTCAAGAGTATCA+AGG + chr8_4:18232647-18232666 None:intergenic 30.0%
TTGATACTCTTGACCATAAT+TGG - chr8_4:18232646-18232665 Msa1317150:intron 30.0%
TTTGATTATGTTGTGATTGG+TGG - chr8_4:18232687-18232706 Msa1317150:intron 30.0%
GAAAGAATTTGACTGATCTA+GGG - chr8_4:18232781-18232800 Msa1317150:CDS 30.0%
TCATTCTTATCCTTTGCAAA+TGG + chr8_4:18233143-18233162 None:intergenic 30.0%
ATGTGACAATAATATGTCCT+TGG - chr8_4:18233256-18233275 Msa1317150:CDS 30.0%
AACGGTAATTATTGTAACCA+AGG + chr8_4:18233276-18233295 None:intergenic 30.0%
ATAATTACCGTTTCGAATTC+AGG - chr8_4:18233284-18233303 Msa1317150:CDS 30.0%
AAAACTTGCAAAAGCAAATG+AGG - chr8_4:18233361-18233380 Msa1317150:CDS 30.0%
TATAGGGAAATGGAAACCAT+TGG + chr8_4:18229960-18229979 None:intergenic 35.0%
!!! CAGAAACAAGTTTTGGAGTT+TGG + chr8_4:18230043-18230062 None:intergenic 35.0%
! GTGGAAACAGAAACAAGTTT+TGG + chr8_4:18230050-18230069 None:intergenic 35.0%
!!! TTGTTTCTGTTTCCACCTTT+TGG - chr8_4:18230054-18230073 Msa1317150:three_prime_UTR 35.0%
!! TTGCTTTTCGTCTTCTCTTT+TGG - chr8_4:18230192-18230211 Msa1317150:three_prime_UTR 35.0%
! TGCTTTTCGTCTTCTCTTTT+GGG - chr8_4:18230193-18230212 Msa1317150:three_prime_UTR 35.0%
! TCTGATTTTGTCTCTGTTTG+AGG + chr8_4:18230272-18230291 None:intergenic 35.0%
!! ATATTCTGGTGCTTTAAGAG+GGG + chr8_4:18230312-18230331 None:intergenic 35.0%
!! CATATTCTGGTGCTTTAAGA+GGG + chr8_4:18230313-18230332 None:intergenic 35.0%
! TCATATTCTGGTGCTTTAAG+AGG + chr8_4:18230314-18230333 None:intergenic 35.0%
GTTTATGCTGGTGTTTCAAA+TGG - chr8_4:18230442-18230461 Msa1317150:CDS 35.0%
! AGGAAAATGTGACCTTTTTG+TGG - chr8_4:18230503-18230522 Msa1317150:CDS 35.0%
! GGAAAATGTGACCTTTTTGT+GGG - chr8_4:18230504-18230523 Msa1317150:CDS 35.0%
AAATCCAATCTCCCACAAAA+AGG + chr8_4:18230518-18230537 None:intergenic 35.0%
CAACTCTCATTTGTGTACAT+TGG + chr8_4:18230555-18230574 None:intergenic 35.0%
!! CAATAAAGGTACCCTAAATC+AGG + chr8_4:18230621-18230640 None:intergenic 35.0%
! CTGATTTAGGGTACCTTTAT+TGG - chr8_4:18230619-18230638 Msa1317150:CDS 35.0%
! ATTTAGGGTACCTTTATTGG+AGG - chr8_4:18230622-18230641 Msa1317150:CDS 35.0%
AACTTCTTAGGGTTGAACTT+TGG + chr8_4:18230672-18230691 None:intergenic 35.0%
AGAAGTTTCTTAGTCTCATG+AGG - chr8_4:18230685-18230704 Msa1317150:CDS 35.0%
TCTCATGAGGAATAAAGCAA+TGG - chr8_4:18230698-18230717 Msa1317150:CDS 35.0%
TGAGATGGAATCACCAATAA+AGG + chr8_4:18230724-18230743 None:intergenic 35.0%
AAGTTAACAATACCTGTGAG+AGG + chr8_4:18230776-18230795 None:intergenic 35.0%
ATTTAAAAACATACTCCCCC+CGG - chr8_4:18231231-18231250 Msa1317150:intron 35.0%
TTTCTTATAAATAGGACCGG+GGG + chr8_4:18231365-18231384 None:intergenic 35.0%
TTTCTTATAAATAGGACCGG+AGG - chr8_4:18231365-18231384 Msa1317150:intron 35.0%
TTCTTATAAATAGGACCGGA+GGG - chr8_4:18231366-18231385 Msa1317150:intron 35.0%
TGAAACACAAGTGTCTTCAA+AGG + chr8_4:18231424-18231443 None:intergenic 35.0%
TTCAAATGTAGTAACAGGCA+TGG - chr8_4:18231516-18231535 Msa1317150:intron 35.0%
! TGTTGATTATTGAAGACAGC+TGG - chr8_4:18231717-18231736 Msa1317150:intron 35.0%
! TGGTGCTAATAACTTATGGT+TGG - chr8_4:18231737-18231756 Msa1317150:intron 35.0%
GGTGCTAATAACTTATGGTT+GGG - chr8_4:18231738-18231757 Msa1317150:intron 35.0%
CTAATAACTTATGGTTGGGT+TGG - chr8_4:18231742-18231761 Msa1317150:intron 35.0%
AACCTTTATGGAAAAGACAG+TGG + chr8_4:18231769-18231788 None:intergenic 35.0%
AGTCACTCCATATTCATTGT+AGG + chr8_4:18231794-18231813 None:intergenic 35.0%
CACAACTTGGTAAACATATG+AGG + chr8_4:18231863-18231882 None:intergenic 35.0%
! CTCATTTCAAGTGCATATTC+TGG - chr8_4:18231898-18231917 Msa1317150:intron 35.0%
! TTTAGAACATATTGTAGGGC+AGG - chr8_4:18232061-18232080 Msa1317150:intron 35.0%
TTAGAACATATTGTAGGGCA+GGG - chr8_4:18232062-18232081 Msa1317150:intron 35.0%
GTTGTAAAGCAAAGAGGAAA+TGG + chr8_4:18232104-18232123 None:intergenic 35.0%
TAGGCAAACCAAGTATTTGT+GGG + chr8_4:18232379-18232398 None:intergenic 35.0%
ATAGGCAAACCAAGTATTTG+TGG + chr8_4:18232380-18232399 None:intergenic 35.0%
ACTTCACACTCTCAGTAATA+TGG - chr8_4:18232550-18232569 Msa1317150:intron 35.0%
GAATGGAGTTTCATCTTCAA+TGG - chr8_4:18232611-18232630 Msa1317150:intron 35.0%
! TGAATTGGTTGGTCCAATTA+TGG + chr8_4:18232662-18232681 None:intergenic 35.0%
CATGAAAACAACACAGTATC+TGG - chr8_4:18232741-18232760 Msa1317150:CDS 35.0%
AGATCAGTCAAATTCTTTCC+AGG + chr8_4:18232780-18232799 None:intergenic 35.0%
GGAAAGAATTTGACTGATCT+AGG - chr8_4:18232780-18232799 Msa1317150:CDS 35.0%
! ATCACTTTGAGAATGGTGAA+TGG - chr8_4:18232901-18232920 Msa1317150:CDS 35.0%
GATGTAGATTCTATCATGAG+AGG - chr8_4:18232984-18233003 Msa1317150:intron 35.0%
! TGGTGTGAACTTGAAACTTT+TGG - chr8_4:18233055-18233074 Msa1317150:intron 35.0%
TTTCATTGCTTAGACCAGAT+GGG - chr8_4:18233090-18233109 Msa1317150:intron 35.0%
! TGTTTACATTGGTGTTTACC+AGG - chr8_4:18233179-18233198 Msa1317150:CDS 35.0%
!! AGATGGTGAAGAGAGTACTA+AGG - chr8_4:18230084-18230103 Msa1317150:three_prime_UTR 40.0%
!! GCAGCATCATTTTTCTTTGC+TGG - chr8_4:18230167-18230186 Msa1317150:three_prime_UTR 40.0%
! GATTTTGTCTCTGTTTGAGG+AGG + chr8_4:18230269-18230288 None:intergenic 40.0%
CAGAGCAAAAACTCTCTGAA+TGG - chr8_4:18230353-18230372 Msa1317150:CDS 40.0%
! ACAAAAGAGTGCACTTGAGT+AGG + chr8_4:18231034-18231053 None:intergenic 40.0%
CTGTAGTTGCAGTTACGATA+TGG - chr8_4:18231162-18231181 Msa1317150:intron 40.0%
TGGTTGTAAAATCTCGACGT+TGG - chr8_4:18231182-18231201 Msa1317150:intron 40.0%
GGTTGTAAAATCTCGACGTT+GGG - chr8_4:18231183-18231202 Msa1317150:intron 40.0%
TTCTTATAAATAGGACCGGG+GGG + chr8_4:18231249-18231268 None:intergenic 40.0%
ACACAAGTGTCTTCAAAGGT+TGG + chr8_4:18231420-18231439 None:intergenic 40.0%
! CAGCTGGTGCTAATAACTTA+TGG - chr8_4:18231733-18231752 Msa1317150:intron 40.0%
! GTCCACTGTCTTTTCCATAA+AGG - chr8_4:18231764-18231783 Msa1317150:intron 40.0%
TCTTTGTCCCCACAAATACT+TGG - chr8_4:18232368-18232387 Msa1317150:intron 40.0%
AGGCAAACCAAGTATTTGTG+GGG + chr8_4:18232378-18232397 None:intergenic 40.0%
! GAGAGTGTGAAGTTATGAGA+GGG + chr8_4:18232543-18232562 None:intergenic 40.0%
! TGAGAGTGTGAAGTTATGAG+AGG + chr8_4:18232544-18232563 None:intergenic 40.0%
GATTATGTTGTGATTGGTGG+TGG - chr8_4:18232690-18232709 Msa1317150:intron 40.0%
!! TTGTGATTGGTGGTGGAAAA+TGG - chr8_4:18232697-18232716 Msa1317150:intron 40.0%
! ACACCAGATCACTTTGAGAA+TGG - chr8_4:18232894-18232913 Msa1317150:CDS 40.0%
TCACCATTCTCAAAGTGATC+TGG + chr8_4:18232900-18232919 None:intergenic 40.0%
GAGAATGGTGAATGGTTTAG+TGG - chr8_4:18232909-18232928 Msa1317150:CDS 40.0%
AATGGTGAATGGTTTAGTGG+AGG - chr8_4:18232912-18232931 Msa1317150:CDS 40.0%
ATGGTGAATGGTTTAGTGGA+GGG - chr8_4:18232913-18232932 Msa1317150:CDS 40.0%
TCGATTTCACCCTCTTTGAA+GGG + chr8_4:18232957-18232976 None:intergenic 40.0%
! ATCGATTTCACCCTCTTTGA+AGG + chr8_4:18232958-18232977 None:intergenic 40.0%
TGAAGTTCAAGAGTTTGCGA+AGG - chr8_4:18233010-18233029 Msa1317150:intron 40.0%
CTTTCATTGCTTAGACCAGA+TGG - chr8_4:18233089-18233108 Msa1317150:intron 40.0%
TCTTATCCTTTGCAAATGGC+TGG + chr8_4:18233139-18233158 None:intergenic 40.0%
! TACCAGGACCAATTGATTCA+TGG - chr8_4:18233195-18233214 Msa1317150:CDS 40.0%
TTCCATGAATCAATTGGTCC+TGG + chr8_4:18233200-18233219 None:intergenic 40.0%
ACAAACTGATTACGTGTTGC+CGG + chr8_4:18233403-18233422 None:intergenic 40.0%
CTACACTTGACAACACCGTT+TGG - chr8_4:18229987-18230006 Msa1317150:three_prime_UTR 45.0%
CATCTTGAGTGACCAAAAGG+TGG + chr8_4:18230069-18230088 None:intergenic 45.0%
! CACCTTTTGGTCACTCAAGA+TGG - chr8_4:18230067-18230086 Msa1317150:three_prime_UTR 45.0%
CACCATCTTGAGTGACCAAA+AGG + chr8_4:18230072-18230091 None:intergenic 45.0%
TTTGTCTCTGTTTGAGGAGG+AGG + chr8_4:18230266-18230285 None:intergenic 45.0%
! GTGACCTTTTTGTGGGAGAT+TGG - chr8_4:18230511-18230530 Msa1317150:CDS 45.0%
! TAGGGTACCTTTATTGGAGG+TGG - chr8_4:18230625-18230644 Msa1317150:CDS 45.0%
TTGGACTCCACCTCCAATAA+AGG + chr8_4:18230635-18230654 None:intergenic 45.0%
TGCAAAGTAGGGACTGAACA+TGG + chr8_4:18230752-18230771 None:intergenic 45.0%
CTGTGAGAGGATGCAAAGTA+GGG + chr8_4:18230763-18230782 None:intergenic 45.0%
!! GTGTCTTCAAAGGTTGGTGA+GGG + chr8_4:18231414-18231433 None:intergenic 45.0%
!! AGTGTCTTCAAAGGTTGGTG+AGG + chr8_4:18231415-18231434 None:intergenic 45.0%
GACCATGCTTCATGATCGAT+GGG + chr8_4:18231618-18231637 None:intergenic 45.0%
TGACCATGCTTCATGATCGA+TGG + chr8_4:18231619-18231638 None:intergenic 45.0%
TGTTGAGGTCTACCATGACA+AGG - chr8_4:18232491-18232510 Msa1317150:intron 45.0%
TCGAACTGTACCCTTCAAAG+AGG - chr8_4:18232944-18232963 Msa1317150:CDS 45.0%
CGAACTGTACCCTTCAAAGA+GGG - chr8_4:18232945-18232964 Msa1317150:CDS 45.0%
TCGTCTCAAGGATCTGAGAA+TGG - chr8_4:18233035-18233054 Msa1317150:intron 45.0%
TTGCAAATGGCTGGAATTGC+CGG + chr8_4:18233130-18233149 None:intergenic 45.0%
GCAATTCCAGCCATTTGCAA+AGG - chr8_4:18233130-18233149 Msa1317150:CDS 45.0%
GTTGAGCTGCATTGTGTACA+TGG - chr8_4:18233321-18233340 Msa1317150:CDS 45.0%
AGGTGACAACACAAATGAGC+CGG - chr8_4:18233381-18233400 Msa1317150:CDS 45.0%
CTGAACATGGTTGCGTGAGA+TGG + chr8_4:18230739-18230758 None:intergenic 50.0%
CCTGTGAGAGGATGCAAAGT+AGG + chr8_4:18230764-18230783 None:intergenic 50.0%
CCTACTTTGCATCCTCTCAC+AGG - chr8_4:18230761-18230780 Msa1317150:CDS 50.0%
GTACACTTGAGTACTCCCTC+CGG + chr8_4:18231384-18231403 None:intergenic 50.0%
AGCCCATCGATCATGAAGCA+TGG - chr8_4:18231613-18231632 Msa1317150:intron 50.0%
TCATGGTAGACCTCAACAGC+TGG + chr8_4:18232489-18232508 None:intergenic 50.0%
TCTGGCTGTCATTACTGTCC+TGG - chr8_4:18232759-18232778 Msa1317150:CDS 50.0%
TTTGCGAAGGCTTCGTCTCA+AGG - chr8_4:18233023-18233042 Msa1317150:intron 50.0%
AATGGCTGGAATTGCCGGTA+AGG + chr8_4:18233125-18233144 None:intergenic 50.0%
GTTTGAGGAGGAGGAGGAGA+AGG + chr8_4:18230257-18230276 None:intergenic 55.0%
GTCTCTGTTTGAGGAGGAGG+AGG + chr8_4:18230263-18230282 None:intergenic 55.0%
ACAGGTGGAACCAGCTGTTG+AGG - chr8_4:18232476-18232495 Msa1317150:intron 55.0%
CTTAGACCAGATGGGCATCC+AGG - chr8_4:18233098-18233117 Msa1317150:intron 55.0%
TAAGGTCCTGGATGCCCATC+TGG + chr8_4:18233107-18233126 None:intergenic 55.0%
ATGGGCATCCAGGACCTTAC+CGG - chr8_4:18233108-18233127 Msa1317150:intron 55.0%
TGGAATTGCCGGTAAGGTCC+TGG + chr8_4:18233119-18233138 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 18229934 18233570 18229934 ID=Msa1317150;Name=Msa1317150
chr8_4 mRNA 18229934 18233570 18229934 ID=Msa1317150-mRNA-1;Parent=Msa1317150;Name=Msa1317150-mRNA-1;_AED=0.05;_eAED=0.05;_QI=152|1|1|1|1|1|3|317|446
chr8_4 exon 18229934 18231024 18229934 ID=Msa1317150-mRNA-1:exon:6744;Parent=Msa1317150-mRNA-1
chr8_4 exon 18232722 18232999 18232722 ID=Msa1317150-mRNA-1:exon:6743;Parent=Msa1317150-mRNA-1
chr8_4 exon 18233130 18233570 18233130 ID=Msa1317150-mRNA-1:exon:6742;Parent=Msa1317150-mRNA-1
chr8_4 five_prime_UTR 18233419 18233570 18233419 ID=Msa1317150-mRNA-1:five_prime_utr;Parent=Msa1317150-mRNA-1
chr8_4 CDS 18233130 18233418 18233130 ID=Msa1317150-mRNA-1:cds;Parent=Msa1317150-mRNA-1
chr8_4 CDS 18232722 18232999 18232722 ID=Msa1317150-mRNA-1:cds;Parent=Msa1317150-mRNA-1
chr8_4 CDS 18230251 18231024 18230251 ID=Msa1317150-mRNA-1:cds;Parent=Msa1317150-mRNA-1
chr8_4 three_prime_UTR 18229934 18230250 18229934 ID=Msa1317150-mRNA-1:three_prime_utr;Parent=Msa1317150-mRNA-1
Gene Sequence

>Msa1317150

ATGGTGAAGAGAGTACTAAGGTCTGAAAATATGAGCTCATTTTTTTCACTGCAAAATCACAACCTTTTCTGTGTTAAATTTGCAGCATCATTTTTCTTTGCTGGTCTTGCTTTTCGTCTTCTCTTTTGGGATTCATTCAGTTTGTCATCATTTGTAGTGATACAGATACCTTCTCCTCCTCCTCCTCAAACAGAGACAAAATCAGAGTCTTCTGTTGTCTCTTCCCCTCTTAAAGCACCAGAATATGATGAATTTCAAGTAAACAATCAGAGCAAAAACTCTCTGAATGGAAAATGTGACCTTTTTGTGGGAGATTGGATTTCTGATCAATCTGAACCAATGTACACAAATGAGAGTTGTCATGTGATTGAACCTCATCAAAATTGTATGAAAAATGGAAGACCTGATTTAGGGTACCTTTATTGGAGGTGGAGTCCAAAAGAGTGTGAATTACCAAAGTTCAACCCTAAGAAGTTTCTTAGTCTCATGAGGAATAAAGCAATGGCCTTTATTGGTGATTCCATCTCACGCAACCATGTTCAGTCCCTACTTTGCATCCTCTCACAGGTGGAACCAGCTGTTGAGGTCTACCATGACAAGGAATATAGATCAAAGATATGGAAATTTCCCTCTCATAACTTCACACTCTCAGTAATATGGACCCCTTTTCTTATTAAAGCAGCTATTTATGAAGACATGAATGGAGTTTCATCTTCAATGGTTCAACTTCACCTTGATACTCTTGACCATAATTGGACCAACCAATTCAACAATTTTGATTATGTTGTGATTGGTGGTGGAAAATGGTTTCTCAAATCTGCAATATTTCATGAAAACAACACAGTATCTGGCTGTCATTACTGTCCTGGAAAGAATTTGACTGATCTAGGGTTCGACTACGCGTACCGAAAAGCATTACAACTTGTTTTCAACTTCTTCACAAAGTCTAGCCATAATGCAACTGTTTTGTTTAGAACTACAACACCAGATCACTTTGAGAATGGTGAATGGTTTAGTGGAGGGTATTGTAATCGAACTGTACCCTTCAAAGAGGGTGAAATCGATATGATAGATGTAGATTCTATCATGAGAGGTATTGAAGTTCAAGAGTTTGCGAAGGCTTCGTCTCAAGGATCTGAGAATGGTGTGAACTTGAAACTTTTGGATACAACTTTTCTTTCATTGCTTAGACCAGATGGGCATCCAGGACCTTACCGGCAATTCCAGCCATTTGCAAAGGATAAGAATGATAAAGTTCAGAATGATTGTTTACATTGGTGTTTACCAGGACCAATTGATTCATGGAATGATATAGTAATGGAAATGCTAGTTAATGCTTAG
Protein sequence

>Msa1317150

MVKRVLRSENMSSFFSLQNHNLFCVKFAASFFFAGLAFRLLFWDSFSLSSFVVIQIPSPPPPQTETKSESSVVSSPLKAPEYDEFQVNNQSKNSLNGKCDLFVGDWISDQSEPMYTNESCHVIEPHQNCMKNGRPDLGYLYWRWSPKECELPKFNPKKFLSLMRNKAMAFIGDSISRNHVQSLLCILSQVEPAVEVYHDKEYRSKIWKFPSHNFTLSVIWTPFLIKAAIYEDMNGVSSSMVQLHLDTLDHNWTNQFNNFDYVVIGGGKWFLKSAIFHENNTVSGCHYCPGKNLTDLGFDYAYRKALQLVFNFFTKSSHNATVLFRTTTPDHFENGEWFSGGYCNRTVPFKEGEIDMIDVDSIMRGIEVQEFAKASSQGSENGVNLKLLDTTFLSLLRPDGHPGPYRQFQPFAKDKNDKVQNDCLHWCLPGPIDSWNDIVMEMLVNA*