AlfalfaGEDB | Msa1323560

M. sativa cultivar zhongmu-4 / Msa1323560

KEGG: -
GO: GO:0001709 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006417 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0032502 GO:0034248 GO:0034249 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045165 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048869 GO:0050779 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:1901363 GO:1903311 GO:1903313 GO:2000112 GO:2000113
PFAM: PF00642.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323560	sp\|Q941Q3\|C3H37_ARATH	52.140	514	194	13	15	502	37	524	2.42e-174	504
Msa1323560	sp\|Q941Q3\|C3H37_ARATH	30.729	192	91	4	315	506	172	321	2.81e-19	94.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323560	A0A072TR50	96.518	517	11	3	1	515	1	512	0.0	1021

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TFs/TRs:

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Gene ID	Type	Classification
Msa1323560	TF	C3H

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1242580	Msa1323560	0.801003	1.105060e-48	-8.615850e-47
Msa1284340	Msa1323560	0.876802	1.044642e-68	-8.615850e-47
Msa1323560	Msa1325860	0.809001	2.347817e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323560	MtrunA17_Chr8g0356241	96.518	517	11	3	1	515	1	512	0.0	1021
Msa1323560	MtrunA17_Chr5g0438891	35.897	351	160	11	143	479	36	335	1.95e-54	188
Msa1323560	MtrunA17_Chr5g0438891	42.169	83	40	2	144	226	263	337	8.06e-11	63.9
Msa1323560	MtrunA17_Chr7g0249221	32.234	394	188	14	116	479	6	350	7.30e-48	171
Msa1323560	MtrunA17_Chr7g0249221	33.163	196	86	3	290	485	3	153	4.75e-27	113
Msa1323560	MtrunA17_Chr7g0249221	49.367	79	35	2	195	268	275	353	5.96e-18	86.3
Msa1323560	MtrunA17_Chr7g0249221	42.000	100	47	4	142	238	274	365	1.57e-13	72.4
Msa1323560	MtrunA17_Chr4g0058011	32.749	342	134	12	195	479	231	533	1.45e-45	168
Msa1323560	MtrunA17_Chr4g0058011	37.209	172	63	4	314	484	231	358	1.48e-25	110
Msa1323560	MtrunA17_Chr4g0058011	36.170	141	72	3	143	274	231	362	1.08e-21	98.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323560	AT3G12680.1	52.140	514	194	13	15	502	37	524	2.46e-175	504
Msa1323560	AT3G12680.1	30.729	192	91	4	315	506	172	321	2.85e-20	94.4
Msa1323560	AT5G16540.3	35.987	314	137	7	194	479	17	294	4.05e-60	202
Msa1323560	AT5G16540.3	33.436	326	131	8	143	430	18	295	2.77e-44	160
Msa1323560	AT5G16540.3	30.890	191	87	4	315	505	19	164	2.74e-25	107
Msa1323560	AT5G16540.3	44.828	87	42	2	393	479	12	92	3.63e-17	83.6
Msa1323560	AT5G16540.3	45.055	91	37	3	139	224	212	294	1.77e-14	75.5
Msa1323560	AT5G16540.1	35.987	314	137	7	194	479	38	315	4.86e-60	202
Msa1323560	AT5G16540.1	33.436	326	131	8	143	430	39	316	3.72e-44	160
Msa1323560	AT5G16540.1	30.890	191	87	4	315	505	40	185	2.68e-25	107

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Find 180 sgRNAs with CRISPR-Local

Find 450 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGCAACGTGCAAGTT+TGG	0.385103	8_4:-31992816	Msa1323560:CDS
AAAACTGGAAAATGTAAATA+CGG	0.484664	8_4:-31994855	Msa1323560:CDS
AAAATTAATACCGGTCTCAC+AGG	0.514093	8_4:+31994660	None:intergenic
AAATCTGGCATATGTAAATT+TGG	0.142400	8_4:-31993338	Msa1323560:CDS
AAATGAGAAAAGGATGGTAT+TGG	0.498703	8_4:+31999111	None:intergenic
AAATGTGCCTGGTTCTGCTC+CGG	0.499853	8_4:-31992691	Msa1323560:CDS
AAATTCTTCTGTGTTTGATG+GGG	0.579820	8_4:-31994748	Msa1323560:CDS
AAATTTGACCATCCACCACC+AGG	0.594941	8_4:-31992783	Msa1323560:CDS
AAGAAGATGAATGAGATATG+AGG	0.493320	8_4:+31999181	None:intergenic
AAGAGGATGTGTTGTAGATT+CGG	0.436282	8_4:+31999055	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAAGATGAATGAGATATG+AGG	+	chr8_4:31992542-31992561	None:intergenic	30.0%
AAATGAGAAAAGGATGGTAT+TGG	+	chr8_4:31992612-31992631	None:intergenic	30.0%
TGATTGATTGATTGTTGGAA+CGG	-	chr8_4:31993106-31993125	Msa1323560:intron	30.0%
TACGCATAAGCATTCAATAA+TGG	+	chr8_4:31993238-31993257	None:intergenic	30.0%
AATGCTTATGCGTAAATGAT+TGG	-	chr8_4:31993244-31993263	Msa1323560:CDS	30.0%
ATGCTTATGCGTAAATGATT+GGG	-	chr8_4:31993245-31993264	Msa1323560:CDS	30.0%
GTTATATAAATAACACCCAG+AGG	+	chr8_4:31993741-31993760	None:intergenic	30.0%
TTCTATGATTATGAGTCAAG+TGG	-	chr8_4:31994060-31994079	Msa1323560:intron	30.0%
CTAACAAGAACCTGTAAATT+TGG	-	chr8_4:31994879-31994898	Msa1323560:intron	30.0%
TTCACCAGATCTACATAAAA+TGG	-	chr8_4:31995470-31995489	Msa1323560:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	31992414	31999328	31992414	ID=Msa1323560;Name=Msa1323560
chr8_4	mRNA	31992414	31999328	31992414	ID=Msa1323560-mRNA-1;Parent=Msa1323560;Name=Msa1323560-mRNA-1;_AED=0.04;_eAED=0.04;_QI=243\|1\|1\|1\|1\|1\|11\|266\|515
chr8_4	exon	31992414	31992865	31992414	ID=Msa1323560-mRNA-1:exon:10431;Parent=Msa1323560-mRNA-1
chr8_4	exon	31993238	31993369	31993238	ID=Msa1323560-mRNA-1:exon:10430;Parent=Msa1323560-mRNA-1
chr8_4	exon	31993762	31993821	31993762	ID=Msa1323560-mRNA-1:exon:10429;Parent=Msa1323560-mRNA-1
chr8_4	exon	31994158	31994288	31994158	ID=Msa1323560-mRNA-1:exon:10428;Parent=Msa1323560-mRNA-1
chr8_4	exon	31994367	31994413	31994367	ID=Msa1323560-mRNA-1:exon:10427;Parent=Msa1323560-mRNA-1
chr8_4	exon	31994517	31994554	31994517	ID=Msa1323560-mRNA-1:exon:10426;Parent=Msa1323560-mRNA-1
chr8_4	exon	31994671	31994886	31994671	ID=Msa1323560-mRNA-1:exon:10425;Parent=Msa1323560-mRNA-1
chr8_4	exon	31995107	31995229	31995107	ID=Msa1323560-mRNA-1:exon:10424;Parent=Msa1323560-mRNA-1
chr8_4	exon	31995325	31995384	31995325	ID=Msa1323560-mRNA-1:exon:10423;Parent=Msa1323560-mRNA-1
chr8_4	exon	31996777	31997015	31996777	ID=Msa1323560-mRNA-1:exon:10422;Parent=Msa1323560-mRNA-1
chr8_4	exon	31998770	31999328	31998770	ID=Msa1323560-mRNA-1:exon:10421;Parent=Msa1323560-mRNA-1
chr8_4	five_prime_UTR	31999086	31999328	31999086	ID=Msa1323560-mRNA-1:five_prime_utr;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31998770	31999085	31998770	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31996777	31997015	31996777	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31995325	31995384	31995325	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31995107	31995229	31995107	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31994671	31994886	31994671	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31994517	31994554	31994517	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31994367	31994413	31994367	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31994158	31994288	31994158	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31993762	31993821	31993762	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31993238	31993369	31993238	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	CDS	31992680	31992865	31992680	ID=Msa1323560-mRNA-1:cds;Parent=Msa1323560-mRNA-1
chr8_4	three_prime_UTR	31992414	31992679	31992414	ID=Msa1323560-mRNA-1:three_prime_utr;Parent=Msa1323560-mRNA-1

Gene Sequence

>Msa1323560

ATGGAAAACCGAATCTACAACACATCCTCTTACAACATCCCAACCGGAGCAGCTCCATCAACTACATCCCCGACCCAATATTACGACCCGAATACAATGTTCCTCGCTCACTATCGCCGCACCGCCGAAGCCGCCGCCGCTGCTTCCTCCATCCGCTACTCCTCCGATTTCGACATTGCTCCTCCTGGAGTTGCGTCTAGAATCTCCACCACTAATCCTTGGGCGAATGCTTTCCCCTCCGCCGCTGCCGCCGTCGCCGCTGCTGCAACAAATGTTGCCTCTGCCGCTTCACTTGATCTTAAACGCTCTTCTGATGCGCTCTACCATCCATCCATTTTGAGCACAATTGGGCAAAACGAAGCTTGGTATACGTCGAACTCACTGGCCAAGCGCCCTAGATATGAAACCGGAAGTGCTTTGCCTATTTATCCACAGAGGCCAGGAGAGAAGGATTGTGCCCACTATATGCTAACAAGAACCTGTAAATTTGGAGAGAGCTGCAAGTTCGATCATCCTATTTGGGTTCCTGCGGGTGGAATCCCAGATTGGAAAGAGGTTCCAAATATTGTTCCAAGTGAAACTCTTCCCGAGAGACCTGGAGAACCAGATTGTCCATTCTTTCTGAAAACTCAAAAATGCAAGTTTGGTACAAAGTGCAAATTTAATCATCCAAATGTTCCCTCTGAAAATGCCGATGTTTCTGGCTTACCTGAGAGGCCACAGGAGCCCCCTTGCGCGTTCTACTTGAAAACTGGAAAATGTAAATACGGTGCTGCATGCAAATTCCATCACCCCAAGGATATCCAGATTCAATTATCTGATGATTTGAGCCGTAATGTTGAGCAGACACAAACAAATTCTTCTGTGTTTGATGGGGCAATAGGAGATACACAGTCAACTATGCCTCTTATATCTCCACTGTCGCATAATAGCAAAGGGCTTCCTGTGAGACCGGGGGAAGTGGATTGTCCATTCTACATGAAAACTGGAAGTTGCAAGTTCGGTGCCAGTTGCCGCTACAATCATCCCGACATGAATGCCATTAATCCACCTGTATCTGCATTGGGCCCTTCTGTTTTGGCATCCTCAACTGCTAATCTTAACATTGGAGCTATTAATCCAGCTGCATCTTTTTATCAAGCTTTTGACCCCAGTCTGTCAAATCAAATGTCTCAGGTGGGAGTAACAGGGACTATCTATCCTCAGCGCCCTGGACAGATTGAATGTGATTTCTATATGAAATCTGGCATATGTAAATTTGGGGAAAGATGCAAGTACCATCACCCAATCGACCGCACAACATCACTGTCAAAACTGCCATCAAATGTTAAACTTACTGCTGCAGGGTTACCAAGAAGAGAGGGTGTTGAGATATGCCCATATTATTTGAAAACTGCAACGTGCAAGTTTGGTGCAACATGCAAATTTGACCATCCACCACCAGGAGAAGTGATGGAGATGGCTAAGTCACAAGGAGCTTCTACAACCAATGGAGGAGAAACGGAGACCAACGAAAATGTGCCTGGTTCTGCTCCGGAACAATATTGA

Protein sequence

>Msa1323560

MENRIYNTSSYNIPTGAAPSTTSPTQYYDPNTMFLAHYRRTAEAAAAASSIRYSSDFDIAPPGVASRISTTNPWANAFPSAAAAVAAAATNVASAASLDLKRSSDALYHPSILSTIGQNEAWYTSNSLAKRPRYETGSALPIYPQRPGEKDCAHYMLTRTCKFGESCKFDHPIWVPAGGIPDWKEVPNIVPSETLPERPGEPDCPFFLKTQKCKFGTKCKFNHPNVPSENADVSGLPERPQEPPCAFYLKTGKCKYGAACKFHHPKDIQIQLSDDLSRNVEQTQTNSSVFDGAIGDTQSTMPLISPLSHNSKGLPVRPGEVDCPFYMKTGSCKFGASCRYNHPDMNAINPPVSALGPSVLASSTANLNIGAINPAASFYQAFDPSLSNQMSQVGVTGTIYPQRPGQIECDFYMKSGICKFGERCKYHHPIDRTTSLSKLPSNVKLTAAGLPRREGVEICPYYLKTATCKFGATCKFDHPPPGEVMEMAKSQGASTTNGGETETNENVPGSAPEQY*