AlfalfaGEDB | Msa1324530

M. sativa cultivar zhongmu-4 / Msa1324530

KEGG: -
GO: GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF01453.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1324530	sp\|Q9LW83\|CE101_ARATH	39.364	818	376	22	32	754	25	817	3.36e-162	494

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1324530	G7LA23	81.509	795	133	5	1	788	1	788	0.0	1306

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0048320	Msa1324530	0.868732	5.245310e-66	-8.615850e-47
Msa1245290	Msa1324530	0.814704	1.345494e-51	-8.615850e-47
Msa1324530	Msa1324610	0.965477	1.176974e-124	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1324530	MtrunA17_Chr8g0358071	78.094	808	146	10	1	788	2	798	1286
Msa1324530	MtrunA17_Chr8g0357961	77.612	804	158	7	1	784	1	802	1283
Msa1324530	MtrunA17_Chr8g0358031	79.695	788	134	7	1	769	2	782	1272
Msa1324530	MtrunA17_Chr8g0357991	77.612	804	159	7	1	784	1	803	1260
Msa1324530	MtrunA17_Chr8g0358081	77.431	802	148	9	1	788	1	783	1245
Msa1324530	MtrunA17_Chr8g0358121	79.452	803	132	8	1	788	1	785	1241
Msa1324530	MtrunA17_Chr8g0358101	76.646	805	152	9	1	788	1	786	1221
Msa1324530	MtrunA17_Chr8g0357881	75.031	805	172	7	1	788	7	799	1193
Msa1324530	MtrunA17_Chr8g0357981	84.069	634	90	2	159	788	1	627	1091
Msa1324530	MtrunA17_Chr8g0357691	56.736	772	292	18	10	756	14	768	812

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1324530	AT1G67520.4	41.427	729	316	18	73	754	79	743	2.09e-163	493
Msa1324530	AT3G16030.1	39.364	818	376	22	32	754	25	817	3.42e-163	494
Msa1324530	AT3G16030.6	40.052	774	354	19	72	754	84	838	5.55e-163	494
Msa1324530	AT1G67520.3	40.872	734	356	19	73	754	79	786	2.02e-160	486
Msa1324530	AT1G67520.6	40.872	734	356	19	73	754	86	793	2.75e-160	486
Msa1324530	AT3G16030.2	40.625	704	311	17	141	754	8	694	1.03e-152	463
Msa1324530	AT1G65790.1	37.113	776	410	18	71	782	77	838	1.85e-148	456
Msa1324530	AT4G21380.1	36.342	831	445	22	19	782	32	845	3.70e-148	456
Msa1324530	AT1G11340.1	36.740	773	393	23	71	769	67	817	1.07e-143	443
Msa1324530	AT4G21390.1	36.550	829	421	23	11	757	13	818	1.14e-143	444

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Find 140 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAACTTGCAGGGAACC+TGG	0.512863	8_4:+34722815	Msa1324530:CDS
AAATAAAGAAACAAATTGTT+TGG	0.308610	8_4:+34721937	Msa1324530:CDS
AAATCAGCTCGAAATTCCCA+TGG	0.640437	8_4:+34723073	Msa1324530:CDS
AAATGACACATTTGTTTCTG+AGG	0.566883	8_4:+34725070	Msa1324530:CDS
AAATTGTTTGGATATACTTA+TGG	0.310580	8_4:+34721949	Msa1324530:CDS
AACAAGACATAATTGGTCAT+TGG	0.352149	8_4:+34722421	Msa1324530:CDS
AACATCTGGACAAGGAATTG+TGG	0.543307	8_4:+34724147	Msa1324530:CDS
AACTGTTGAGTTTACGTCTA+CGG	0.436467	8_4:-34722165	None:intergenic
AACTTGCAGGGAACCTGGTG+AGG	0.570811	8_4:+34722820	Msa1324530:CDS
AAGATAGGAAGTTTGGACAA+TGG	0.659010	8_4:+34722666	Msa1324530:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTTATTCTCCTTTGGATT+TGG	+	chr8_4:34721859-34721878	Msa1324530:intron	30.0%
AATATACTCACCAAATCCAA+AGG	-	chr8_4:34721872-34721891	None:intergenic	30.0%
TTTGGATATACTTATGGTTG+TGG	+	chr8_4:34721955-34721974	Msa1324530:CDS	30.0%
ATAAATACTGTTAATGGCAC+AGG	+	chr8_4:34722116-34722135	Msa1324530:CDS	30.0%
TAATTCCCAACTTCATTGTT+GGG	-	chr8_4:34722394-34722413	None:intergenic	30.0%
TTAATTCCCAACTTCATTGT+TGG	-	chr8_4:34722395-34722414	None:intergenic	30.0%
AACAAGACATAATTGGTCAT+TGG	+	chr8_4:34722421-34722440	Msa1324530:CDS	30.0%
ACAAGACATAATTGGTCATT+GGG	+	chr8_4:34722422-34722441	Msa1324530:CDS	30.0%
ACTGATAAACTACCAAATTC+AGG	+	chr8_4:34722455-34722474	Msa1324530:CDS	30.0%
AGACTAAATTCACCTGAATT+TGG	-	chr8_4:34722470-34722489	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	34721800	34725514	34721800	ID=Msa1324530;Name=Msa1324530
chr8_4	mRNA	34721800	34725514	34721800	ID=Msa1324530-mRNA-1;Parent=Msa1324530;Name=Msa1324530-mRNA-1;_AED=0.40;_eAED=0.40;_QI=94\|1\|1\|1\|0.85\|0.75\|8\|180\|788
chr8_4	exon	34721800	34721869	34721800	ID=Msa1324530-mRNA-1:exon:11035;Parent=Msa1324530-mRNA-1
chr8_4	exon	34721900	34723094	34721900	ID=Msa1324530-mRNA-1:exon:11036;Parent=Msa1324530-mRNA-1
chr8_4	exon	34723310	34723417	34723310	ID=Msa1324530-mRNA-1:exon:11037;Parent=Msa1324530-mRNA-1
chr8_4	exon	34723527	34723696	34723527	ID=Msa1324530-mRNA-1:exon:11038;Parent=Msa1324530-mRNA-1
chr8_4	exon	34724100	34724310	34724100	ID=Msa1324530-mRNA-1:exon:11039;Parent=Msa1324530-mRNA-1
chr8_4	exon	34724475	34724712	34724475	ID=Msa1324530-mRNA-1:exon:11040;Parent=Msa1324530-mRNA-1
chr8_4	exon	34724791	34724941	34724791	ID=Msa1324530-mRNA-1:exon:11041;Parent=Msa1324530-mRNA-1
chr8_4	exon	34725017	34725514	34725017	ID=Msa1324530-mRNA-1:exon:11042;Parent=Msa1324530-mRNA-1
chr8_4	five_prime_UTR	34721800	34721869	34721800	ID=Msa1324530-mRNA-1:five_prime_utr;Parent=Msa1324530-mRNA-1
chr8_4	five_prime_UTR	34721900	34721923	34721900	ID=Msa1324530-mRNA-1:five_prime_utr;Parent=Msa1324530-mRNA-1
chr8_4	CDS	34721924	34723094	34721924	ID=Msa1324530-mRNA-1:cds;Parent=Msa1324530-mRNA-1
chr8_4	CDS	34723310	34723417	34723310	ID=Msa1324530-mRNA-1:cds;Parent=Msa1324530-mRNA-1
chr8_4	CDS	34723527	34723696	34723527	ID=Msa1324530-mRNA-1:cds;Parent=Msa1324530-mRNA-1
chr8_4	CDS	34724100	34724310	34724100	ID=Msa1324530-mRNA-1:cds;Parent=Msa1324530-mRNA-1
chr8_4	CDS	34724475	34724712	34724475	ID=Msa1324530-mRNA-1:cds;Parent=Msa1324530-mRNA-1
chr8_4	CDS	34724791	34724941	34724791	ID=Msa1324530-mRNA-1:cds;Parent=Msa1324530-mRNA-1
chr8_4	CDS	34725017	34725334	34725017	ID=Msa1324530-mRNA-1:cds;Parent=Msa1324530-mRNA-1
chr8_4	three_prime_UTR	34725335	34725514	34725335	ID=Msa1324530-mRNA-1:three_prime_utr;Parent=Msa1324530-mRNA-1

Gene Sequence

>Msa1324530

ATGATAAGCTTTAAAATAAAGAAACAAATTGTTTGGATATACTTATGGTTGTGGTTGAGTACTACTGCTAATATTTGTGTTAAAGCAACAAATGATAGTTTGAAGCCAGGTGATACATTGAATTCCAATTCCTCGCTATGTTCTAAACAAGGCATGTTTTGTCTTTTAATCGACGGTGATGGTTACTTGACTATAAATACTGTTAATGGCACAGGAGTGTGGATTTATAATGGGAATCGACCCGTAGACGTAAACTCAACAGTTTTATCACTAGACTACACTGGAGTGCTCAAAATAGAATCTCAAAATAGGAAATTATCAATTATCATTTATTCTTCACCTCAACCCTCCAACAAGACTGTGGCAACTATGTTAGACACGGGTAACTTTGTGCTTCAACAACTTCACCCAAATGGGACAAAGAATATATTATGGCAGAGTTTTGATTATCCCACAGACACTTTGATCCCAACAATGAAGTTGGGAATTAATCGAAAAACAAGACATAATTGGTCATTGGGTTCATGGTTGACTGATAAACTACCAAATTCAGGTGAATTTAGTCTTGAATGGGAACCAAAACAAGGTGAATTGAACATTAAGAGACGCGGAAAAGTGTATTGGAAAAGCGGAAAACTTAAAAGTAATGGAATATTCGAGAATATACCAGCCAATGTTCAACATATGTATCAGTACATTATTGTCTCTAATAAGGATGAAGATTCTTTTGCTTTCGAAGTCAAAGATAGGAAGTTTGGACAATGGTACATTACCTCGAAGGGGACGTTGGTAGCTGATGATGGAGTAAGTGCTAGTAGTAAGACAGATATTGGAAATGCAGATATGTGTTATGGATATAATAGTGATGGAGGGTGCCAAAAATGGGAGGATAAACCAACTTGCAGGGAACCTGGTGAGGTTTTTCAGATAAAGGCGGGAACACCATACAAAAACAATATAACAACCATTGAACGTGATGTGACTTATGGTTACAGTGATTGCAAGATGAGATGTTGGAGAAATTGTAATTGCAATGCATTCCAAGAATTATATGAAAATTTGACCGGATGTGTATTCTATTCATGGAATTCGACACAAGATGGTAATTTGGATGGTCGGTTATCCTTTTATGCCTTGGTAAAGCCAACAAAATCAGCTCGAAATTCCCATGGTAAAAAAAAGTGGATATGGATAGGTTCTGCGATCGCAGCTGCTTTACTCATATTATGCCCAATCATTTTGTGCTTAGCCAAAAAGAAACAAAAATATGCACTACAAGATAGGAAAAGTAAAAGAAAGGATCTTGAATACTATAATCTCAAAGATCTTGAAGATGACTTCAAGGAACATGATATAAAAGTTTTTAACTTTACATCAATTTTGGAGGCAACTATGGACTTTTCACCTGAAAACAAGTTAGGACATGGAGGCTATGGACCCGTTTATAAGGGAATTTTGGCAACAGGGCAAGAAGTTGCTGTAAAAAGACTTTCAAAAACATCTGGACAAGGAATTGTGGAATTCAAAAATGAATTGGTACTTATATGTGAACTTCAGCACACAAATCTTGTACAACTACTTGGTTGTTGCATTCATGAAGAAGAAAGGATTCTAATTTACGAATATATGCCTAACAAAAGCTTGGATTTCTATCTATTTGATTGTACAAAAAAGAAGCTGCTAGATTGGAAGAAACGTTTTAACATAATTGAAGGAATTGCTCAAGGATTGCTCTATCTTCACAAGTATTCAAGACTCAAAATCATTCATAGAGACTTGAAGGCTAGCAACATACTTCTTGATGAGAATATGAACCCAAAAATTTCTGATTTTGGCATGGCAAGAATGTTCACACAACAGGAATCCGTAGTAAATACCAACAGGATTGTTGGGACATATGGATATATGTCTCCAGAATATGCCATGGAAGGAGTTTGTTCTACAAAGTCTGACGTCTATAGCTTTGGAGTGTTGCTTCTTGAAATCGTTTGTGGGAGAAAAAACAATAGCTTCTATGATGTTGATCGCCCACTAAATCTAATAGGACAAGCATGGGAGTTATGGAATGATGGTGAGTATCTAAAGCTAATGGATCCAACATTAAATGACACATTTGTTTCTGAGGAAGTGCAAAGGTGCATTCATGTTGGTCTCTTATGTGTAGAGCATTATGCAATTGATCGACCTACAATGTCTGATGTTATATCTGTGTTAACAAACAAATATGAACTCACAAACTTGCCAAGAAGACCGGCATTTTATGTTAGAAGAGAAATCTTTGAGGGGGAAACAACTTCCAAAGGGCAAGACACCGACACGTATTCTACGACAGCAATTTCTATGTCCTGTGAAGAAGGGAGCATATAA

Protein sequence

>Msa1324530

MISFKIKKQIVWIYLWLWLSTTANICVKATNDSLKPGDTLNSNSSLCSKQGMFCLLIDGDGYLTINTVNGTGVWIYNGNRPVDVNSTVLSLDYTGVLKIESQNRKLSIIIYSSPQPSNKTVATMLDTGNFVLQQLHPNGTKNILWQSFDYPTDTLIPTMKLGINRKTRHNWSLGSWLTDKLPNSGEFSLEWEPKQGELNIKRRGKVYWKSGKLKSNGIFENIPANVQHMYQYIIVSNKDEDSFAFEVKDRKFGQWYITSKGTLVADDGVSASSKTDIGNADMCYGYNSDGGCQKWEDKPTCREPGEVFQIKAGTPYKNNITTIERDVTYGYSDCKMRCWRNCNCNAFQELYENLTGCVFYSWNSTQDGNLDGRLSFYALVKPTKSARNSHGKKKWIWIGSAIAAALLILCPIILCLAKKKQKYALQDRKSKRKDLEYYNLKDLEDDFKEHDIKVFNFTSILEATMDFSPENKLGHGGYGPVYKGILATGQEVAVKRLSKTSGQGIVEFKNELVLICELQHTNLVQLLGCCIHEEERILIYEYMPNKSLDFYLFDCTKKKLLDWKKRFNIIEGIAQGLLYLHKYSRLKIIHRDLKASNILLDENMNPKISDFGMARMFTQQESVVNTNRIVGTYGYMSPEYAMEGVCSTKSDVYSFGVLLLEIVCGRKNNSFYDVDRPLNLIGQAWELWNDGEYLKLMDPTLNDTFVSEEVQRCIHVGLLCVEHYAIDRPTMSDVISVLTNKYELTNLPRRPAFYVRREIFEGETTSKGQDTDTYSTTAISMSCEEGSI*