AlfalfaGEDB | Msa1328930

M. sativa cultivar zhongmu-4 / Msa1328930

KEGG: -
GO: GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF10533.12

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1328930	sp\|O22176\|WRK15_ARATH	51.404	356	115	15	1	337	1	317	7.25e-92	280

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1328930	A0A072UP79	98.817	338	3	1	1	337	1	338	0.0	681

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TFs/TRs:

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Gene ID	Type	Classification
Msa1328930	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1208110	Msa1328930	0.848184	7.205631e-60	-8.615850e-47
Msa1256330	Msa1328930	0.909628	4.617042e-82	-8.615850e-47
Msa1296520	Msa1328930	0.914759	1.314750e-84	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1328930	MtrunA17_Chr4g0053281	98.817	338	3	1	1	337	1	338	0.0	681
Msa1328930	MtrunA17_Chr5g0401821	57.817	339	73	9	1	337	1	271	1.53e-113	330
Msa1328930	MtrunA17_Chr1g0147881	40.204	393	126	15	1	337	1	340	9.44e-70	221
Msa1328930	MtrunA17_Chr4g0071521	44.892	323	145	11	20	333	15	313	2.02e-67	214
Msa1328930	MtrunA17_Chr1g0184691	52.198	182	65	4	151	323	183	351	5.18e-48	165
Msa1328930	MtrunA17_Chr3g0139021	55.639	133	57	1	199	331	250	380	5.49e-43	153
Msa1328930	MtrunA17_Chr7g0228941	62.921	89	32	1	246	334	33	120	4.55e-34	121
Msa1328930	MtrunA17_Chr2g0284321	55.172	87	37	2	239	324	9	94	3.89e-27	106
Msa1328930	MtrunA17_Chr7g0270051	55.814	86	34	1	241	322	25	110	8.09e-27	106
Msa1328930	MtrunA17_Chr2g0319961	58.242	91	31	3	240	324	50	139	9.92e-27	106

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1328930	AT2G23320.1	51.404	356	115	15	1	337	1	317	7.37e-93	280
Msa1328930	AT4G24240.1	45.238	378	115	14	1	324	1	340	5.82e-85	261
Msa1328930	AT4G31550.1	47.429	350	117	16	1	326	1	307	4.18e-81	250
Msa1328930	AT4G31550.2	46.857	350	118	16	1	326	1	306	1.14e-79	246
Msa1328930	AT2G24570.1	44.242	330	115	13	20	322	13	300	1.15e-70	223
Msa1328930	AT2G23320.2	46.488	299	107	14	1	285	1	260	6.56e-60	193
Msa1328930	AT5G28650.1	42.568	296	99	10	85	323	39	320	1.21e-56	187
Msa1328930	AT3G04670.1	42.667	300	93	13	85	323	39	320	1.97e-56	186
Msa1328930	AT4G24240.2	40.118	339	111	14	1	285	1	301	1.09e-53	178
Msa1328930	AT2G30590.1	76.147	109	22	3	216	322	264	370	2.52e-52	177

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Find 85 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTAAAGAAAAGAAAGT+GGG	0.531483	8_4:+46962684	Msa1328930:three_prime_UTR
AAGATGGTGGATTTGGTAAA+CGG	0.429455	8_4:+46962649	Msa1328930:three_prime_UTR
AAGTGTAGCTCTGAAACTTT+GGG	0.416277	8_4:+46961725	Msa1328930:CDS
AAGTTAAAGAAAAGAAAGTG+GGG	0.621689	8_4:+46962685	Msa1328930:three_prime_UTR
AATAGTCAAGATGGTGGATT+TGG	0.345890	8_4:+46962642	Msa1328930:three_prime_UTR
AATGAAGAAGAAGAGAGTGG+TGG	0.649002	8_4:-46961695	None:intergenic
AATGGTAGTAAATTGTTGGT+TGG	0.464968	8_4:+46962536	Msa1328930:three_prime_UTR
AATTGAAAGTACCTTGGATG+AGG	0.611960	8_4:-46962050	None:intergenic
ACAACCGTCGATGTCGTCGC+CGG	0.604344	8_4:+46961622	Msa1328930:CDS
ACAAGTGCAGTAGTGTGAGA+GGG	0.679618	8_4:+46962362	Msa1328930:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAGAGAGAGAAAATAAAAC+GGG	-	chr8_4:46961011-46961030	None:intergenic	30.0%
TGAAATAACACGTTTGAACT+TGG	-	chr8_4:46961336-46961355	None:intergenic	30.0%
CATTTCTCTTGATTATAGGA+CGG	-	chr8_4:46961520-46961539	None:intergenic	30.0%
TCTCTTCTTCTTCATTGAAA+AGG	+	chr8_4:46961702-46961721	Msa1328930:CDS	30.0%
GTTGTCATTGTTCCAAAAAA+AGG	+	chr8_4:46961777-46961796	Msa1328930:CDS	30.0%
TAGGAAAATGAGATTGAAGA+GGG	+	chr8_4:46961932-46961951	Msa1328930:CDS	30.0%
TTTCTCCAAGAATAATCATC+TGG	-	chr8_4:46962002-46962021	None:intergenic	30.0%
TATGGTCAGAAACCTATTAA+AGG	+	chr8_4:46962023-46962042	Msa1328930:CDS	30.0%
ATAAATAGGTTCATACGTAG+GGG	-	chr8_4:46962107-46962126	None:intergenic	30.0%
AATGGTAGTAAATTGTTGGT+TGG	+	chr8_4:46962536-46962555	Msa1328930:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	46960974	46962707	46960974	ID=Msa1328930;Name=Msa1328930
chr8_4	mRNA	46960974	46962707	46960974	ID=Msa1328930-mRNA-1;Parent=Msa1328930;Name=Msa1328930-mRNA-1;_AED=0.02;_eAED=0.02;_QI=100\|1\|1\|1\|0.5\|0.66\|3\|194\|337
chr8_4	exon	46960974	46961798	46960974	ID=Msa1328930-mRNA-1:exon:13449;Parent=Msa1328930-mRNA-1
chr8_4	exon	46961932	46962060	46961932	ID=Msa1328930-mRNA-1:exon:13450;Parent=Msa1328930-mRNA-1
chr8_4	exon	46962354	46962707	46962354	ID=Msa1328930-mRNA-1:exon:13451;Parent=Msa1328930-mRNA-1
chr8_4	five_prime_UTR	46960974	46961073	46960974	ID=Msa1328930-mRNA-1:five_prime_utr;Parent=Msa1328930-mRNA-1
chr8_4	CDS	46961074	46961798	46961074	ID=Msa1328930-mRNA-1:cds;Parent=Msa1328930-mRNA-1
chr8_4	CDS	46961932	46962060	46961932	ID=Msa1328930-mRNA-1:cds;Parent=Msa1328930-mRNA-1
chr8_4	CDS	46962354	46962513	46962354	ID=Msa1328930-mRNA-1:cds;Parent=Msa1328930-mRNA-1
chr8_4	three_prime_UTR	46962514	46962707	46962514	ID=Msa1328930-mRNA-1:three_prime_utr;Parent=Msa1328930-mRNA-1

Gene Sequence

>Msa1328930

ATGGCCGTGGAGCTCATGACAGGCTACAACAGAAACCAAGCTTTCACAACCAAAGCAGAAGAAAATGCTGTCCAAGAAGCAGCTTCTGGTTTAGAAAGTGTTGAAAAACTCATAAAACTTCTCTCAGAAGCACGTCACAAATACCAACCTTCTTCATCTTCTTCCTCTTCCTTTTCACCTTCAAACCCTAACAACAATAACAACAACGCTACTATGGATATAGAAAGAGATTGCAAAGCTGTTGCAGACATAGCTGTTTCCAAGTTCAAACGTGTTATTTCACTGCTTGAAAAAACAAGAACTGGTCATGCTCGTTTCAGAAAAGCACCTTTACCACAACCACCACCACCATCACAAACCCTACAAGTTCAACCTTCTGAACCAATGATTTTCAACGCTACACCTTTGCAACAGATTCCACCAACTGTTTCTACTACCCTTCACCGTCCTATAATCAAGAGAAATGATTCTTCTAAAACTCTTAATTTTTCATACTCTTCTGCTGGTAATTCTTTCATTTCTTCACTCACTGGCGACGACAACAACAAACAACCGTCGATGTCGTCGCCGGCGGGTGCTTTTCAGATTACAAATCTGTCTCATGTTTCATCTGTTGGTAAGCCACCACTCTCTTCTTCTTCATTGAAAAGGAAGTGTAGCTCTGAAACTTTGGGTTCTGGAAAGTGTGGTAGTTCTTCTAGCCGTTGTCATTGTTCCAAAAAAAGTAGGAAAATGAGATTGAAGAGGGTTGTTAGGGTTCCTGCAATTAGCTTGAAGATGGCTGATATTCCACCAGATGATTATTCTTGGAGAAAATATGGTCAGAAACCTATTAAAGGTTCACCTCATCCAAGGGGATACTACAAGTGCAGTAGTGTGAGAGGGTGTCCAGCAAGAAAGCATGTAGAGAGAGCTCTTGATGATGCTTCTATGCTGGTGGTTACCTATGAAGGAGAGCACAATCACTCTCTTTCTGCAGCTGATGCTAGCAATCTTATTTTAGAATCATCATAG

Protein sequence

>Msa1328930

MAVELMTGYNRNQAFTTKAEENAVQEAASGLESVEKLIKLLSEARHKYQPSSSSSSSFSPSNPNNNNNNATMDIERDCKAVADIAVSKFKRVISLLEKTRTGHARFRKAPLPQPPPPSQTLQVQPSEPMIFNATPLQQIPPTVSTTLHRPIIKRNDSSKTLNFSYSSAGNSFISSLTGDDNNKQPSMSSPAGAFQITNLSHVSSVGKPPLSSSSLKRKCSSETLGSGKCGSSSSRCHCSKKSRKMRLKRVVRVPAISLKMADIPPDDYSWRKYGQKPIKGSPHPRGYYKCSSVRGCPARKHVERALDDASMLVVTYEGEHNHSLSAADASNLILESS*