AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1339260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1339260 MtrunA17_Chr4g0035651 96.429 224 7 1 1 223 1 224 3.15e-161 446
Msa1339260 MtrunA17_Chr3g0145061 59.624 213 69 6 1 198 1 211 7.06e-77 232
Msa1339260 MtrunA17_Chr1g0155811 47.926 217 99 4 1 208 1 212 3.42e-57 184
Msa1339260 MtrunA17_Chr3g0142221 47.191 178 58 3 1 177 1 143 3.71e-51 166
Msa1339260 MtrunA17_Chr2g0314671 50.794 126 58 3 1 126 1 122 5.20e-36 129
Msa1339260 MtrunA17_Chr4g0033641 50.000 110 54 1 1 110 1 109 1.75e-34 124
Msa1339260 MtrunA17_Chr2g0329511 48.182 110 56 1 1 110 1 109 7.74e-32 117
Msa1339260 MtrunA17_Chr5g0405151 37.324 142 78 4 1 135 1 138 5.26e-23 92.8
Msa1339260 MtrunA17_Chr1g0150471 43.299 97 45 1 5 101 6 92 8.79e-20 86.7
Msa1339260 MtrunA17_Chr3g0136701 39.175 97 49 1 5 101 6 92 9.17e-17 78.2
Msa1339260 MtrunA17_Chr4g0072871 40.860 93 45 1 5 97 22 104 1.86e-16 78.2
Msa1339260 MtrunA17_Chr7g0220721 35.043 117 63 2 5 121 26 129 2.17e-15 75.1
Msa1339260 MtrunA17_Chr5g0429341 36.082 97 52 1 1 97 1 87 6.18e-14 70.9
Msa1339260 MtrunA17_Chr3g0120711 35.052 97 52 2 1 97 1 86 2.22e-11 63.2
Msa1339260 MtrunA17_Chr7g0268691 32.653 98 55 2 5 102 20 106 8.07e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1339260 AT4G39070.1 53.000 200 70 4 1 176 1 200 6.81e-59 187
Msa1339260 AT1G75540.1 36.562 320 132 10 1 250 1 319 1.68e-54 179
Msa1339260 AT1G06040.2 46.715 137 66 2 1 137 1 130 1.04e-36 128
Msa1339260 AT1G78600.1 51.724 116 55 1 1 116 1 115 2.43e-36 131
Msa1339260 AT1G78600.2 42.529 174 91 3 1 173 1 166 3.53e-36 131
Msa1339260 AT1G06040.1 46.715 137 66 2 1 137 1 130 9.80e-36 128
Msa1339260 AT2G31380.1 51.327 113 54 1 1 113 1 112 1.72e-33 122
Msa1339260 AT4G10240.1 44.138 145 67 4 1 135 1 141 1.90e-30 112
Msa1339260 AT4G38960.1 43.810 105 55 2 1 105 1 101 2.14e-23 94.0
Msa1339260 AT4G38960.6 43.810 105 55 2 1 105 1 101 2.14e-23 94.0
Msa1339260 AT2G21320.1 43.810 105 55 2 1 105 1 101 4.33e-22 90.5
Msa1339260 AT2G24790.2 41.237 97 47 1 5 101 8 94 5.18e-19 83.2
Msa1339260 AT2G24790.1 41.237 97 47 1 5 101 8 94 1.29e-18 83.6
Msa1339260 AT5G24930.1 37.113 97 51 1 5 101 6 92 2.73e-16 77.8
Msa1339260 AT5G15850.1 32.520 123 70 2 5 127 12 121 1.02e-15 76.3
Msa1339260 AT4G38960.3 31.081 148 55 3 1 105 1 144 1.99e-15 73.6
Msa1339260 AT5G15840.2 33.333 123 70 2 5 127 20 130 6.43e-15 73.2
Msa1339260 AT5G15840.1 33.333 123 70 2 5 127 20 130 1.07e-14 73.2
Msa1339260 AT3G02380.1 36.559 93 49 1 5 97 16 98 1.10e-14 73.2
Msa1339260 AT2G47890.2 40.244 82 39 1 5 86 13 84 2.56e-12 65.1
Msa1339260 AT1G28050.1 35.644 101 55 1 2 102 6 96 2.64e-12 66.6
Msa1339260 AT2G47890.3 40.244 82 39 1 5 86 13 84 3.65e-12 65.5
Msa1339260 AT2G47890.1 40.244 82 39 1 5 86 13 84 5.25e-12 65.1
Msa1339260 AT5G48250.1 34.694 98 53 2 1 97 1 88 5.87e-12 65.5
Msa1339260 AT2G33500.1 34.000 100 56 1 3 102 10 99 1.05e-11 64.7
Msa1339260 AT2G33500.2 34.000 100 56 1 3 102 10 99 1.10e-11 64.7
Msa1339260 AT5G57660.1 34.646 127 60 4 5 122 22 134 1.14e-11 64.3
Msa1339260 AT3G07650.3 36.047 86 45 1 1 86 1 76 6.07e-11 62.4
Msa1339260 AT3G07650.2 36.047 86 45 1 1 86 1 76 6.07e-11 62.4
Msa1339260 AT3G07650.4 36.047 86 45 1 1 86 1 76 6.07e-11 62.4
Msa1339260 AT3G07650.1 36.047 86 45 1 1 86 1 76 6.07e-11 62.4

Find 88 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGGATCTGATGAATTA+TGG 0.212412 tig0003042:-138500 None:intergenic
AATTGTTTCAATCAAGTATT+CGG 0.289976 tig0003042:-139482 None:intergenic
GGATGGGGACAAAAGAATTA+TGG 0.300907 tig0003042:-138629 None:intergenic
ACCTTACAGAAAACGTTATT+AGG 0.333966 tig0003042:-139547 None:intergenic
ATCACAGAGAGGGGTGTCTT+TGG 0.337373 tig0003042:-138860 None:intergenic
CACCTTTACTACATTAACTT+TGG 0.337392 tig0003042:+138596 Msa1339260:five_prime_UTR
CTTGAAAATGAACTTGGTCT+TGG 0.353565 tig0003042:-139385 None:intergenic
AAGGTCTTCCATGCAGTAAC+CGG 0.360314 tig0003042:-139506 None:intergenic
TGTTTATAACTTGAAGATCT+TGG 0.361700 tig0003042:-139716 None:intergenic
CTGAGCCATTGGAAGTTGTT+GGG 0.365142 tig0003042:-139345 None:intergenic
TGGTAAGTTTGTTGACTTCA+TGG 0.370579 tig0003042:-139261 None:intergenic
TTGTTCTCATCTAAGGTAGA+AGG 0.378807 tig0003042:+140007 Msa1339260:three_prime_UTR
AAGATAGAGCAATACTTTGC+AGG 0.379068 tig0003042:+139221 Msa1339260:CDS
TACTTGATTGAAACAATTCC+CGG 0.387327 tig0003042:+139487 Msa1339260:CDS
TCTGAGCCATTGGAAGTTGT+TGG 0.394596 tig0003042:-139346 None:intergenic
CAGAGAGCTGCTTCATCTGC+AGG 0.413650 tig0003042:-138757 None:intergenic
GAGAGAGTCAGAAAATAAAT+AGG 0.421018 tig0003042:+138359 Msa1339260:five_prime_UTR
ATTGGGATCTGATGAATTAT+GGG 0.421290 tig0003042:-138499 None:intergenic
CAGAGAGAACAGCTAAGATT+TGG 0.425768 tig0003042:-138447 None:intergenic
GTGTGCTATTTCTACAACAT+TGG 0.430641 tig0003042:-138517 None:intergenic
CCCCACATTGATTCTTTAGA+CGG 0.435559 tig0003042:+139801 Msa1339260:CDS
CCTCTACATTGTTTGAAGAA+TGG 0.436801 tig0003042:-139885 None:intergenic
ATGGATAAGAAAACAAATGA+TGG 0.444047 tig0003042:-139952 None:intergenic
GTGTAAAAGAAGCATCAAAA+AGG 0.446259 tig0003042:-139525 None:intergenic
AAGTTATAAACATGTCTGAC+TGG 0.451524 tig0003042:+139727 Msa1339260:CDS
TGTGCTATTTCTACAACATT+GGG 0.452647 tig0003042:-138516 None:intergenic
AACTTACCAAGCAACACAAC+AGG 0.456566 tig0003042:+139275 Msa1339260:CDS
TTGAAGGCAAAGTGGGGATG+GGG 0.458406 tig0003042:-138644 None:intergenic
CAACATACTAAAGTTGTTGA+AGG 0.460895 tig0003042:+139837 Msa1339260:CDS
TGAACTTGGTCTTGGACTTG+AGG 0.462812 tig0003042:-139377 None:intergenic
GTGTTTAGCAGAGACCTTGT+TGG 0.467471 tig0003042:-138809 None:intergenic
CTTCTCACCGGCGTAAAGAT+CGG 0.467524 tig0003042:+139301 Msa1339260:CDS
CAGAGACCTTGTTGGCATAG+TGG 0.468511 tig0003042:-138801 None:intergenic
CCCACATTGATTCTTTAGAC+GGG 0.468902 tig0003042:+139802 Msa1339260:CDS
TTCTCTTTCTATGAGTTAAG+AGG 0.469449 tig0003042:-138327 None:intergenic
ATAATTCGTACATATGAAAC+GGG 0.469720 tig0003042:-140067 None:intergenic
GATAATTCGTACATATGAAA+CGG 0.470506 tig0003042:-140068 None:intergenic
TTTGAAGGCAAAGTGGGGAT+GGG 0.471662 tig0003042:-138645 None:intergenic
GATGGGGACAAAAGAATTAT+GGG 0.472346 tig0003042:-138628 None:intergenic
TCTGATGAATTATGGGCGTT+TGG 0.473178 tig0003042:-138492 None:intergenic
GAGAGAAGTACATAATTAAG+AGG 0.479528 tig0003042:-140034 None:intergenic
CGTACATATGAAACGGGAAA+AGG 0.480675 tig0003042:-140061 None:intergenic
CAAGTATTCGGAAATGCTAC+TGG 0.481122 tig0003042:-139470 None:intergenic
TTCCAAAGTTAATGTAGTAA+AGG 0.489803 tig0003042:-138598 None:intergenic
GGTTCTTAGTTCTGAGCCAT+TGG 0.490954 tig0003042:-139356 None:intergenic
AAGAATGGAGGACTAATTGA+AGG 0.493324 tig0003042:-139870 None:intergenic
AGAGGGGTGTCTTTGGAAGT+GGG 0.493908 tig0003042:-138853 None:intergenic
GAGAGGGGTGTCTTTGGAAG+TGG 0.504462 tig0003042:-138854 None:intergenic
GCCATTGATTTAAAATCGAT+CGG 0.523753 tig0003042:+138564 Msa1339260:five_prime_UTR
AAGAAACCTGTTGTGTTGCT+TGG 0.533532 tig0003042:-139281 None:intergenic
AGATAGAGCAATACTTTGCA+GGG 0.534009 tig0003042:+139222 Msa1339260:CDS
AACAATTCCCGGTTACTGCA+TGG 0.543090 tig0003042:+139498 Msa1339260:CDS
AAAATAAATAGGTCTCGTAA+TGG 0.547529 tig0003042:+138370 Msa1339260:five_prime_UTR
TTCATCTGCAGGGCAGAACA+AGG 0.547884 tig0003042:-138746 None:intergenic
TGGATAAGAAAACAAATGAT+GGG 0.550535 tig0003042:-139951 None:intergenic
CACAACAGGTTTCTTCTCAC+CGG 0.551440 tig0003042:+139289 Msa1339260:CDS
AATTATGGGCGTTTGGTGTG+AGG 0.551532 tig0003042:-138485 None:intergenic
TCTCTTTCTATGAGTTAAGA+GGG 0.553179 tig0003042:-138326 None:intergenic
TCGAATTAGCGCTAAGCTGA+GGG 0.554345 tig0003042:-139773 None:intergenic
AAGTTCTTTCAAAGAAAACA+TGG 0.562402 tig0003042:+139429 Msa1339260:CDS
TCAAACCCAACAACTTCCAA+TGG 0.565279 tig0003042:+139340 Msa1339260:CDS
AGTTTGTTGACTTCATGGAT+AGG 0.572223 tig0003042:-139256 None:intergenic
AGGTCTTCCATGCAGTAACC+GGG 0.572681 tig0003042:-139505 None:intergenic
GAGGGGTGTCTTTGGAAGTG+GGG 0.580604 tig0003042:-138852 None:intergenic
GGGTGTCTTTGGAAGTGGGG+TGG 0.581849 tig0003042:-138849 None:intergenic
CTACATTGTTTGAAGAATGG+AGG 0.582130 tig0003042:-139882 None:intergenic
TTCGAATTAGCGCTAAGCTG+AGG 0.584714 tig0003042:-139774 None:intergenic
AGAGAGCTGCTTCATCTGCA+GGG 0.589609 tig0003042:-138756 None:intergenic
ATATGAAACGGGAAAAGGAA+AGG 0.590584 tig0003042:-140056 None:intergenic
GGGAATCTAACTTGAGATTG+TGG 0.592001 tig0003042:-139753 None:intergenic
CCCGTCTAAAGAATCAATGT+GGG 0.595981 tig0003042:-139802 None:intergenic
TTACACGCATTAGTTGATCT+CGG 0.600288 tig0003042:+138542 Msa1339260:five_prime_UTR
CAATATCCACTATGCCAACA+AGG 0.603596 tig0003042:+138795 Msa1339260:CDS
CCACATTGATTCTTTAGACG+GGG 0.606501 tig0003042:+139803 Msa1339260:CDS
CCTTGCAGATATCACAGAGA+GGG 0.606602 tig0003042:-138870 None:intergenic
CCCTCTCTGTGATATCTGCA+AGG 0.608181 tig0003042:+138870 Msa1339260:CDS
CCCCGTCTAAAGAATCAATG+TGG 0.617525 tig0003042:-139803 None:intergenic
GTTTGTGACATGCATCACAC+TGG 0.618408 tig0003042:-138714 None:intergenic
TGATGCATGTCACAAACAAG+AGG 0.622158 tig0003042:+138720 Msa1339260:CDS
AGTTATAAACATGTCTGACT+GGG 0.637692 tig0003042:+139728 Msa1339260:CDS
CCATTCTTCAAACAATGTAG+AGG 0.655261 tig0003042:+139885 Msa1339260:CDS
TCTCATCTAAGGTAGAAGGT+AGG 0.670836 tig0003042:+140011 Msa1339260:three_prime_UTR
CATAGAAAGAGAATCACACA+TGG 0.673771 tig0003042:+138337 Msa1339260:five_prime_UTR
ATCTGCAGGGCAGAACAAGG+AGG 0.684576 tig0003042:-138743 None:intergenic
CTTGCAGATATCACAGAGAG+GGG 0.687405 tig0003042:-138869 None:intergenic
AGAAGCACCGATCTTTACGC+CGG 0.690841 tig0003042:-139308 None:intergenic
CCGTCTAAAGAATCAATGTG+GGG 0.706719 tig0003042:-139801 None:intergenic
ACCTTGCAGATATCACAGAG+AGG 0.718667 tig0003042:-138871 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTGTTTATCAAAAAAAT+AGG + tig0003042:139065-139084 Msa1339260:intron 15.0%
!!! ATAACATTTTTATATATTGC+AGG + tig0003042:139171-139190 Msa1339260:intron 15.0%
!! CTACTCAATTAATTAAAATA+AGG + tig0003042:139649-139668 Msa1339260:intron 15.0%
!! TTCTAGATAAAAATTGTAAA+TGG - tig0003042:139974-139993 None:intergenic 15.0%
!! AAGTTGTTTATCAAAAAAAT+AGG + tig0003042:139065-139084 Msa1339260:intron 15.0%
!!! ATAACATTTTTATATATTGC+AGG + tig0003042:139171-139190 Msa1339260:intron 15.0%
!! CTACTCAATTAATTAAAATA+AGG + tig0003042:139649-139668 Msa1339260:intron 15.0%
!! TTCTAGATAAAAATTGTAAA+TGG - tig0003042:139974-139993 None:intergenic 15.0%
!!! AACTAATATGACCATATTTT+TGG - tig0003042:139116-139135 None:intergenic 20.0%
!! AATTGTTTCAATCAAGTATT+CGG - tig0003042:139485-139504 None:intergenic 20.0%
!!! AATAAGGTGTATATGTTTTT+TGG + tig0003042:139665-139684 Msa1339260:intron 20.0%
!!! AACTAATATGACCATATTTT+TGG - tig0003042:139116-139135 None:intergenic 20.0%
!! AATTGTTTCAATCAAGTATT+CGG - tig0003042:139485-139504 None:intergenic 20.0%
!!! AATAAGGTGTATATGTTTTT+TGG + tig0003042:139665-139684 Msa1339260:intron 20.0%
! AAAATAAATAGGTCTCGTAA+TGG + tig0003042:138370-138389 Msa1339260:five_prime_UTR 25.0%
! ATCTGACTTTCAGAAAAATT+TGG - tig0003042:138403-138422 None:intergenic 25.0%
! TTCCAAAGTTAATGTAGTAA+AGG - tig0003042:138601-138620 None:intergenic 25.0%
! AATAAAAGACTGAGTGATAT+AGG + tig0003042:139146-139165 Msa1339260:intron 25.0%
!! ATTTTTATATATTGCAGGAG+AGG + tig0003042:139176-139195 Msa1339260:intron 25.0%
!! TTTTCACTTGAAAATGAACT+TGG - tig0003042:139394-139413 None:intergenic 25.0%
! AAGTTCTTTCAAAGAAAACA+TGG + tig0003042:139429-139448 Msa1339260:CDS 25.0%
!!! ATGTTTTTTGGTATTGTTTC+AGG + tig0003042:139677-139696 Msa1339260:intron 25.0%
! TGTTTATAACTTGAAGATCT+TGG - tig0003042:139719-139738 None:intergenic 25.0%
! TGGATAAGAAAACAAATGAT+GGG - tig0003042:139954-139973 None:intergenic 25.0%
! ATGGATAAGAAAACAAATGA+TGG - tig0003042:139955-139974 None:intergenic 25.0%
! AAAATAAATAGGTCTCGTAA+TGG + tig0003042:138370-138389 Msa1339260:five_prime_UTR 25.0%
! ATCTGACTTTCAGAAAAATT+TGG - tig0003042:138403-138422 None:intergenic 25.0%
! TTCCAAAGTTAATGTAGTAA+AGG - tig0003042:138601-138620 None:intergenic 25.0%
! AATAAAAGACTGAGTGATAT+AGG + tig0003042:139146-139165 Msa1339260:intron 25.0%
!! ATTTTTATATATTGCAGGAG+AGG + tig0003042:139176-139195 Msa1339260:intron 25.0%
!! TTTTCACTTGAAAATGAACT+TGG - tig0003042:139394-139413 None:intergenic 25.0%
! AAGTTCTTTCAAAGAAAACA+TGG + tig0003042:139429-139448 Msa1339260:CDS 25.0%
!!! ATGTTTTTTGGTATTGTTTC+AGG + tig0003042:139677-139696 Msa1339260:intron 25.0%
! TGTTTATAACTTGAAGATCT+TGG - tig0003042:139719-139738 None:intergenic 25.0%
! TGGATAAGAAAACAAATGAT+GGG - tig0003042:139954-139973 None:intergenic 25.0%
! ATGGATAAGAAAACAAATGA+TGG - tig0003042:139955-139974 None:intergenic 25.0%
TCTCTTTCTATGAGTTAAGA+GGG - tig0003042:138329-138348 None:intergenic 30.0%
TTCTCTTTCTATGAGTTAAG+AGG - tig0003042:138330-138349 None:intergenic 30.0%
GAGAGAGTCAGAAAATAAAT+AGG + tig0003042:138359-138378 Msa1339260:five_prime_UTR 30.0%
ATTGGGATCTGATGAATTAT+GGG - tig0003042:138502-138521 None:intergenic 30.0%
!! TGTGCTATTTCTACAACATT+GGG - tig0003042:138519-138538 None:intergenic 30.0%
!! GCCATTGATTTAAAATCGAT+CGG + tig0003042:138564-138583 Msa1339260:five_prime_UTR 30.0%
CACCTTTACTACATTAACTT+TGG + tig0003042:138596-138615 Msa1339260:five_prime_UTR 30.0%
!!! TTTTGTTTTGAAGGCAAAGT+GGG - tig0003042:138654-138673 None:intergenic 30.0%
!!! TTTTTGTTTTGAAGGCAAAG+TGG - tig0003042:138655-138674 None:intergenic 30.0%
!!! AGGTTTGGTTTTTGTTTTGA+AGG - tig0003042:138663-138682 None:intergenic 30.0%
AAGTACGTAAGAAATAGAGA+AGG - tig0003042:138902-138921 None:intergenic 30.0%
! TTTCTTACGTACTTTTCAAC+AGG + tig0003042:138908-138927 Msa1339260:intron 30.0%
! TTCTTACGTACTTTTCAACA+GGG + tig0003042:138909-138928 Msa1339260:intron 30.0%
CGCTGAAAATCAGTTAAATA+AGG - tig0003042:139017-139036 None:intergenic 30.0%
!!! ACCATATTTTTGGATCGTAT+AGG - tig0003042:139106-139125 None:intergenic 30.0%
TACTTGATTGAAACAATTCC+CGG + tig0003042:139487-139506 Msa1339260:CDS 30.0%
!! GTGTAAAAGAAGCATCAAAA+AGG - tig0003042:139528-139547 None:intergenic 30.0%
! ACCTAATAACGTTTTCTGTA+AGG + tig0003042:139546-139565 Msa1339260:CDS 30.0%
ACCTTACAGAAAACGTTATT+AGG - tig0003042:139550-139569 None:intergenic 30.0%
!! AACGTTTTCTGTAAGGTATT+TGG + tig0003042:139553-139572 Msa1339260:intron 30.0%
! AAAAGAAAAAAGTGCACTGT+TGG - tig0003042:139582-139601 None:intergenic 30.0%
TAGATGAAAAATAGCTAGCT+AGG - tig0003042:139632-139651 None:intergenic 30.0%
AAGTTATAAACATGTCTGAC+TGG + tig0003042:139727-139746 Msa1339260:CDS 30.0%
AGTTATAAACATGTCTGACT+GGG + tig0003042:139728-139747 Msa1339260:CDS 30.0%
CAACATACTAAAGTTGTTGA+AGG + tig0003042:139837-139856 Msa1339260:CDS 30.0%
! TGCAATTTTGTTCTCATCTA+AGG + tig0003042:140000-140019 Msa1339260:three_prime_UTR 30.0%
! GAGAGAAGTACATAATTAAG+AGG - tig0003042:140037-140056 None:intergenic 30.0%
TCTCTTTCTATGAGTTAAGA+GGG - tig0003042:138329-138348 None:intergenic 30.0%
TTCTCTTTCTATGAGTTAAG+AGG - tig0003042:138330-138349 None:intergenic 30.0%
GAGAGAGTCAGAAAATAAAT+AGG + tig0003042:138359-138378 Msa1339260:five_prime_UTR 30.0%
ATTGGGATCTGATGAATTAT+GGG - tig0003042:138502-138521 None:intergenic 30.0%
!! TGTGCTATTTCTACAACATT+GGG - tig0003042:138519-138538 None:intergenic 30.0%
!! GCCATTGATTTAAAATCGAT+CGG + tig0003042:138564-138583 Msa1339260:five_prime_UTR 30.0%
CACCTTTACTACATTAACTT+TGG + tig0003042:138596-138615 Msa1339260:five_prime_UTR 30.0%
!!! TTTTGTTTTGAAGGCAAAGT+GGG - tig0003042:138654-138673 None:intergenic 30.0%
!!! TTTTTGTTTTGAAGGCAAAG+TGG - tig0003042:138655-138674 None:intergenic 30.0%
!!! AGGTTTGGTTTTTGTTTTGA+AGG - tig0003042:138663-138682 None:intergenic 30.0%
AAGTACGTAAGAAATAGAGA+AGG - tig0003042:138902-138921 None:intergenic 30.0%
! TTTCTTACGTACTTTTCAAC+AGG + tig0003042:138908-138927 Msa1339260:intron 30.0%
! TTCTTACGTACTTTTCAACA+GGG + tig0003042:138909-138928 Msa1339260:intron 30.0%
CGCTGAAAATCAGTTAAATA+AGG - tig0003042:139017-139036 None:intergenic 30.0%
!!! ACCATATTTTTGGATCGTAT+AGG - tig0003042:139106-139125 None:intergenic 30.0%
TACTTGATTGAAACAATTCC+CGG + tig0003042:139487-139506 Msa1339260:CDS 30.0%
!! GTGTAAAAGAAGCATCAAAA+AGG - tig0003042:139528-139547 None:intergenic 30.0%
! ACCTAATAACGTTTTCTGTA+AGG + tig0003042:139546-139565 Msa1339260:CDS 30.0%
ACCTTACAGAAAACGTTATT+AGG - tig0003042:139550-139569 None:intergenic 30.0%
!! AACGTTTTCTGTAAGGTATT+TGG + tig0003042:139553-139572 Msa1339260:intron 30.0%
! AAAAGAAAAAAGTGCACTGT+TGG - tig0003042:139582-139601 None:intergenic 30.0%
TAGATGAAAAATAGCTAGCT+AGG - tig0003042:139632-139651 None:intergenic 30.0%
AAGTTATAAACATGTCTGAC+TGG + tig0003042:139727-139746 Msa1339260:CDS 30.0%
AGTTATAAACATGTCTGACT+GGG + tig0003042:139728-139747 Msa1339260:CDS 30.0%
CAACATACTAAAGTTGTTGA+AGG + tig0003042:139837-139856 Msa1339260:CDS 30.0%
! TGCAATTTTGTTCTCATCTA+AGG + tig0003042:140000-140019 Msa1339260:three_prime_UTR 30.0%
! GAGAGAAGTACATAATTAAG+AGG - tig0003042:140037-140056 None:intergenic 30.0%
CATAGAAAGAGAATCACACA+TGG + tig0003042:138337-138356 Msa1339260:five_prime_UTR 35.0%
CATTGGGATCTGATGAATTA+TGG - tig0003042:138503-138522 None:intergenic 35.0%
!! GTGTGCTATTTCTACAACAT+TGG - tig0003042:138520-138539 None:intergenic 35.0%
TTACACGCATTAGTTGATCT+CGG + tig0003042:138542-138561 Msa1339260:five_prime_UTR 35.0%
!! GCCGATCGATTTTAAATCAA+TGG - tig0003042:138568-138587 None:intergenic 35.0%
GATGGGGACAAAAGAATTAT+GGG - tig0003042:138631-138650 None:intergenic 35.0%
!!! TTTGTTTTGAAGGCAAAGTG+GGG - tig0003042:138653-138672 None:intergenic 35.0%
!!! TCTATTTTTGAGCGTTTTGG+AGG - tig0003042:138683-138702 None:intergenic 35.0%
!!! CTCTCTATTTTTGAGCGTTT+TGG - tig0003042:138686-138705 None:intergenic 35.0%
GCCTATACGATCCAAAAATA+TGG + tig0003042:139102-139121 Msa1339260:intron 35.0%
AAGATAGAGCAATACTTTGC+AGG + tig0003042:139221-139240 Msa1339260:CDS 35.0%
AGATAGAGCAATACTTTGCA+GGG + tig0003042:139222-139241 Msa1339260:CDS 35.0%
!! AGTTTGTTGACTTCATGGAT+AGG - tig0003042:139259-139278 None:intergenic 35.0%
!! TGGTAAGTTTGTTGACTTCA+TGG - tig0003042:139264-139283 None:intergenic 35.0%
CTTGAAAATGAACTTGGTCT+TGG - tig0003042:139388-139407 None:intergenic 35.0%
AAGAATGGAGGACTAATTGA+AGG - tig0003042:139873-139892 None:intergenic 35.0%
CTACATTGTTTGAAGAATGG+AGG - tig0003042:139885-139904 None:intergenic 35.0%
CCTCTACATTGTTTGAAGAA+TGG - tig0003042:139888-139907 None:intergenic 35.0%
CCATTCTTCAAACAATGTAG+AGG + tig0003042:139885-139904 Msa1339260:CDS 35.0%
! TTGTTCTCATCTAAGGTAGA+AGG + tig0003042:140007-140026 Msa1339260:three_prime_UTR 35.0%
ATATGAAACGGGAAAAGGAA+AGG - tig0003042:140059-140078 None:intergenic 35.0%
CATAGAAAGAGAATCACACA+TGG + tig0003042:138337-138356 Msa1339260:five_prime_UTR 35.0%
CATTGGGATCTGATGAATTA+TGG - tig0003042:138503-138522 None:intergenic 35.0%
!! GTGTGCTATTTCTACAACAT+TGG - tig0003042:138520-138539 None:intergenic 35.0%
TTACACGCATTAGTTGATCT+CGG + tig0003042:138542-138561 Msa1339260:five_prime_UTR 35.0%
!! GCCGATCGATTTTAAATCAA+TGG - tig0003042:138568-138587 None:intergenic 35.0%
GATGGGGACAAAAGAATTAT+GGG - tig0003042:138631-138650 None:intergenic 35.0%
!!! TTTGTTTTGAAGGCAAAGTG+GGG - tig0003042:138653-138672 None:intergenic 35.0%
!!! TCTATTTTTGAGCGTTTTGG+AGG - tig0003042:138683-138702 None:intergenic 35.0%
!!! CTCTCTATTTTTGAGCGTTT+TGG - tig0003042:138686-138705 None:intergenic 35.0%
GCCTATACGATCCAAAAATA+TGG + tig0003042:139102-139121 Msa1339260:intron 35.0%
AAGATAGAGCAATACTTTGC+AGG + tig0003042:139221-139240 Msa1339260:CDS 35.0%
AGATAGAGCAATACTTTGCA+GGG + tig0003042:139222-139241 Msa1339260:CDS 35.0%
!! AGTTTGTTGACTTCATGGAT+AGG - tig0003042:139259-139278 None:intergenic 35.0%
!! TGGTAAGTTTGTTGACTTCA+TGG - tig0003042:139264-139283 None:intergenic 35.0%
CTTGAAAATGAACTTGGTCT+TGG - tig0003042:139388-139407 None:intergenic 35.0%
AAGAATGGAGGACTAATTGA+AGG - tig0003042:139873-139892 None:intergenic 35.0%
CTACATTGTTTGAAGAATGG+AGG - tig0003042:139885-139904 None:intergenic 35.0%
CCTCTACATTGTTTGAAGAA+TGG - tig0003042:139888-139907 None:intergenic 35.0%
CCATTCTTCAAACAATGTAG+AGG + tig0003042:139885-139904 Msa1339260:CDS 35.0%
! TTGTTCTCATCTAAGGTAGA+AGG + tig0003042:140007-140026 Msa1339260:three_prime_UTR 35.0%
ATATGAAACGGGAAAAGGAA+AGG - tig0003042:140059-140078 None:intergenic 35.0%
CAGAGAGAACAGCTAAGATT+TGG - tig0003042:138450-138469 None:intergenic 40.0%
TCTGATGAATTATGGGCGTT+TGG - tig0003042:138495-138514 None:intergenic 40.0%
GGATGGGGACAAAAGAATTA+TGG - tig0003042:138632-138651 None:intergenic 40.0%
!!! TTTTGAGCGTTTTGGAGGTT+TGG - tig0003042:138678-138697 None:intergenic 40.0%
TGATGCATGTCACAAACAAG+AGG + tig0003042:138720-138739 Msa1339260:CDS 40.0%
CAATATCCACTATGCCAACA+AGG + tig0003042:138795-138814 Msa1339260:CDS 40.0%
AACTTACCAAGCAACACAAC+AGG + tig0003042:139275-139294 Msa1339260:CDS 40.0%
! AAGAAACCTGTTGTGTTGCT+TGG - tig0003042:139284-139303 None:intergenic 40.0%
TCAAACCCAACAACTTCCAA+TGG + tig0003042:139340-139359 Msa1339260:CDS 40.0%
CAAGTATTCGGAAATGCTAC+TGG - tig0003042:139473-139492 None:intergenic 40.0%
GGGAATCTAACTTGAGATTG+TGG - tig0003042:139756-139775 None:intergenic 40.0%
CCGTCTAAAGAATCAATGTG+GGG - tig0003042:139804-139823 None:intergenic 40.0%
!! CCCCACATTGATTCTTTAGA+CGG + tig0003042:139801-139820 Msa1339260:CDS 40.0%
CCCGTCTAAAGAATCAATGT+GGG - tig0003042:139805-139824 None:intergenic 40.0%
!! CCCACATTGATTCTTTAGAC+GGG + tig0003042:139802-139821 Msa1339260:CDS 40.0%
!! CCACATTGATTCTTTAGACG+GGG + tig0003042:139803-139822 Msa1339260:CDS 40.0%
! TCTCATCTAAGGTAGAAGGT+AGG + tig0003042:140011-140030 Msa1339260:three_prime_UTR 40.0%
CGTACATATGAAACGGGAAA+AGG - tig0003042:140064-140083 None:intergenic 40.0%
CAGAGAGAACAGCTAAGATT+TGG - tig0003042:138450-138469 None:intergenic 40.0%
TCTGATGAATTATGGGCGTT+TGG - tig0003042:138495-138514 None:intergenic 40.0%
GGATGGGGACAAAAGAATTA+TGG - tig0003042:138632-138651 None:intergenic 40.0%
!!! TTTTGAGCGTTTTGGAGGTT+TGG - tig0003042:138678-138697 None:intergenic 40.0%
TGATGCATGTCACAAACAAG+AGG + tig0003042:138720-138739 Msa1339260:CDS 40.0%
CAATATCCACTATGCCAACA+AGG + tig0003042:138795-138814 Msa1339260:CDS 40.0%
AACTTACCAAGCAACACAAC+AGG + tig0003042:139275-139294 Msa1339260:CDS 40.0%
! AAGAAACCTGTTGTGTTGCT+TGG - tig0003042:139284-139303 None:intergenic 40.0%
TCAAACCCAACAACTTCCAA+TGG + tig0003042:139340-139359 Msa1339260:CDS 40.0%
CAAGTATTCGGAAATGCTAC+TGG - tig0003042:139473-139492 None:intergenic 40.0%
GGGAATCTAACTTGAGATTG+TGG - tig0003042:139756-139775 None:intergenic 40.0%
CCGTCTAAAGAATCAATGTG+GGG - tig0003042:139804-139823 None:intergenic 40.0%
!! CCCCACATTGATTCTTTAGA+CGG + tig0003042:139801-139820 Msa1339260:CDS 40.0%
CCCGTCTAAAGAATCAATGT+GGG - tig0003042:139805-139824 None:intergenic 40.0%
!! CCCACATTGATTCTTTAGAC+GGG + tig0003042:139802-139821 Msa1339260:CDS 40.0%
!! CCACATTGATTCTTTAGACG+GGG + tig0003042:139803-139822 Msa1339260:CDS 40.0%
! TCTCATCTAAGGTAGAAGGT+AGG + tig0003042:140011-140030 Msa1339260:three_prime_UTR 40.0%
CGTACATATGAAACGGGAAA+AGG - tig0003042:140064-140083 None:intergenic 40.0%
!! AATTATGGGCGTTTGGTGTG+AGG - tig0003042:138488-138507 None:intergenic 45.0%
TTTGAAGGCAAAGTGGGGAT+GGG - tig0003042:138648-138667 None:intergenic 45.0%
!! TTTTGAAGGCAAAGTGGGGA+TGG - tig0003042:138649-138668 None:intergenic 45.0%
GTTTGTGACATGCATCACAC+TGG - tig0003042:138717-138736 None:intergenic 45.0%
GTGTTTAGCAGAGACCTTGT+TGG - tig0003042:138812-138831 None:intergenic 45.0%
CTTGCAGATATCACAGAGAG+GGG - tig0003042:138872-138891 None:intergenic 45.0%
CCTTGCAGATATCACAGAGA+GGG - tig0003042:138873-138892 None:intergenic 45.0%
ACCTTGCAGATATCACAGAG+AGG - tig0003042:138874-138893 None:intergenic 45.0%
CACAACAGGTTTCTTCTCAC+CGG + tig0003042:139289-139308 Msa1339260:CDS 45.0%
CTGAGCCATTGGAAGTTGTT+GGG - tig0003042:139348-139367 None:intergenic 45.0%
TCTGAGCCATTGGAAGTTGT+TGG - tig0003042:139349-139368 None:intergenic 45.0%
!! GGTTCTTAGTTCTGAGCCAT+TGG - tig0003042:139359-139378 None:intergenic 45.0%
!! TGAACTTGGTCTTGGACTTG+AGG - tig0003042:139380-139399 None:intergenic 45.0%
AACAATTCCCGGTTACTGCA+TGG + tig0003042:139498-139517 Msa1339260:CDS 45.0%
AAGGTCTTCCATGCAGTAAC+CGG - tig0003042:139509-139528 None:intergenic 45.0%
TCGAATTAGCGCTAAGCTGA+GGG - tig0003042:139776-139795 None:intergenic 45.0%
TTCGAATTAGCGCTAAGCTG+AGG - tig0003042:139777-139796 None:intergenic 45.0%
CCCCGTCTAAAGAATCAATG+TGG - tig0003042:139806-139825 None:intergenic 45.0%
!! AATTATGGGCGTTTGGTGTG+AGG - tig0003042:138488-138507 None:intergenic 45.0%
TTTGAAGGCAAAGTGGGGAT+GGG - tig0003042:138648-138667 None:intergenic 45.0%
!! TTTTGAAGGCAAAGTGGGGA+TGG - tig0003042:138649-138668 None:intergenic 45.0%
GTTTGTGACATGCATCACAC+TGG - tig0003042:138717-138736 None:intergenic 45.0%
GTGTTTAGCAGAGACCTTGT+TGG - tig0003042:138812-138831 None:intergenic 45.0%
CTTGCAGATATCACAGAGAG+GGG - tig0003042:138872-138891 None:intergenic 45.0%
CCTTGCAGATATCACAGAGA+GGG - tig0003042:138873-138892 None:intergenic 45.0%
ACCTTGCAGATATCACAGAG+AGG - tig0003042:138874-138893 None:intergenic 45.0%
CACAACAGGTTTCTTCTCAC+CGG + tig0003042:139289-139308 Msa1339260:CDS 45.0%
CTGAGCCATTGGAAGTTGTT+GGG - tig0003042:139348-139367 None:intergenic 45.0%
TCTGAGCCATTGGAAGTTGT+TGG - tig0003042:139349-139368 None:intergenic 45.0%
!! GGTTCTTAGTTCTGAGCCAT+TGG - tig0003042:139359-139378 None:intergenic 45.0%
!! TGAACTTGGTCTTGGACTTG+AGG - tig0003042:139380-139399 None:intergenic 45.0%
AACAATTCCCGGTTACTGCA+TGG + tig0003042:139498-139517 Msa1339260:CDS 45.0%
AAGGTCTTCCATGCAGTAAC+CGG - tig0003042:139509-139528 None:intergenic 45.0%
TCGAATTAGCGCTAAGCTGA+GGG - tig0003042:139776-139795 None:intergenic 45.0%
TTCGAATTAGCGCTAAGCTG+AGG - tig0003042:139777-139796 None:intergenic 45.0%
CCCCGTCTAAAGAATCAATG+TGG - tig0003042:139806-139825 None:intergenic 45.0%
TTGAAGGCAAAGTGGGGATG+GGG - tig0003042:138647-138666 None:intergenic 50.0%
TTCATCTGCAGGGCAGAACA+AGG - tig0003042:138749-138768 None:intergenic 50.0%
AGAGAGCTGCTTCATCTGCA+GGG - tig0003042:138759-138778 None:intergenic 50.0%
CAGAGACCTTGTTGGCATAG+TGG - tig0003042:138804-138823 None:intergenic 50.0%
!! AGAGGGGTGTCTTTGGAAGT+GGG - tig0003042:138856-138875 None:intergenic 50.0%
!! ATCACAGAGAGGGGTGTCTT+TGG - tig0003042:138863-138882 None:intergenic 50.0%
CCCTCTCTGTGATATCTGCA+AGG + tig0003042:138870-138889 Msa1339260:CDS 50.0%
CTTCTCACCGGCGTAAAGAT+CGG + tig0003042:139301-139320 Msa1339260:CDS 50.0%
!! AGAAGCACCGATCTTTACGC+CGG - tig0003042:139311-139330 None:intergenic 50.0%
AGGTCTTCCATGCAGTAACC+GGG - tig0003042:139508-139527 None:intergenic 50.0%
TTGAAGGCAAAGTGGGGATG+GGG - tig0003042:138647-138666 None:intergenic 50.0%
TTCATCTGCAGGGCAGAACA+AGG - tig0003042:138749-138768 None:intergenic 50.0%
AGAGAGCTGCTTCATCTGCA+GGG - tig0003042:138759-138778 None:intergenic 50.0%
CAGAGACCTTGTTGGCATAG+TGG - tig0003042:138804-138823 None:intergenic 50.0%
!! AGAGGGGTGTCTTTGGAAGT+GGG - tig0003042:138856-138875 None:intergenic 50.0%
!! ATCACAGAGAGGGGTGTCTT+TGG - tig0003042:138863-138882 None:intergenic 50.0%
CCCTCTCTGTGATATCTGCA+AGG + tig0003042:138870-138889 Msa1339260:CDS 50.0%
CTTCTCACCGGCGTAAAGAT+CGG + tig0003042:139301-139320 Msa1339260:CDS 50.0%
!! AGAAGCACCGATCTTTACGC+CGG - tig0003042:139311-139330 None:intergenic 50.0%
AGGTCTTCCATGCAGTAACC+GGG - tig0003042:139508-139527 None:intergenic 50.0%
ATCTGCAGGGCAGAACAAGG+AGG - tig0003042:138746-138765 None:intergenic 55.0%
CAGAGAGCTGCTTCATCTGC+AGG - tig0003042:138760-138779 None:intergenic 55.0%
!! GAGGGGTGTCTTTGGAAGTG+GGG - tig0003042:138855-138874 None:intergenic 55.0%
!! GAGAGGGGTGTCTTTGGAAG+TGG - tig0003042:138857-138876 None:intergenic 55.0%
CTGCAACACAACCGCAGTAG+AGG + tig0003042:138982-139001 Msa1339260:intron 55.0%
GGCTGATACTGCCTCTACTG+CGG - tig0003042:138996-139015 None:intergenic 55.0%
ATCTGCAGGGCAGAACAAGG+AGG - tig0003042:138746-138765 None:intergenic 55.0%
CAGAGAGCTGCTTCATCTGC+AGG - tig0003042:138760-138779 None:intergenic 55.0%
!! GAGGGGTGTCTTTGGAAGTG+GGG - tig0003042:138855-138874 None:intergenic 55.0%
!! GAGAGGGGTGTCTTTGGAAG+TGG - tig0003042:138857-138876 None:intergenic 55.0%
CTGCAACACAACCGCAGTAG+AGG + tig0003042:138982-139001 Msa1339260:intron 55.0%
GGCTGATACTGCCTCTACTG+CGG - tig0003042:138996-139015 None:intergenic 55.0%
! GGGTGTCTTTGGAAGTGGGG+TGG - tig0003042:138852-138871 None:intergenic 60.0%
! GGGTGTCTTTGGAAGTGGGG+TGG - tig0003042:138852-138871 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0003042 gene 138326 140087 138326 ID=Msa1339260;Name=Msa1339260
tig0003042 mRNA 138326 140087 138326 ID=Msa1339260-mRNA-1;Parent=Msa1339260;Name=Msa1339260-mRNA-1;_AED=0.05;_eAED=0.05;_QI=380|1|1|1|1|1|3|170|259
tig0003042 exon 138326 138891 138326 ID=Msa1339260-mRNA-1:exon:1981;Parent=Msa1339260-mRNA-1
tig0003042 exon 139193 139567 139193 ID=Msa1339260-mRNA-1:exon:1982;Parent=Msa1339260-mRNA-1
tig0003042 exon 139699 140087 139699 ID=Msa1339260-mRNA-1:exon:1983;Parent=Msa1339260-mRNA-1
tig0003042 five_prime_UTR 138326 138705 138326 ID=Msa1339260-mRNA-1:five_prime_utr;Parent=Msa1339260-mRNA-1
tig0003042 CDS 138706 138891 138706 ID=Msa1339260-mRNA-1:cds;Parent=Msa1339260-mRNA-1
tig0003042 CDS 139193 139567 139193 ID=Msa1339260-mRNA-1:cds;Parent=Msa1339260-mRNA-1
tig0003042 CDS 139699 139917 139699 ID=Msa1339260-mRNA-1:cds;Parent=Msa1339260-mRNA-1
tig0003042 three_prime_UTR 139918 140087 139918 ID=Msa1339260-mRNA-1:three_prime_utr;Parent=Msa1339260-mRNA-1
Gene Sequence

>Msa1339260

ATGAAGATCCAGTGTGATGCATGTCACAAACAAGAGGCCTCCTTGTTCTGCCCTGCAGATGAAGCAGCTCTCTGCAATCAATGTGATCGCAATATCCACTATGCCAACAAGGTCTCTGCTAAACACAAACGCTTCACTCTTCACCACCCCACTTCCAAAGACACCCCTCTCTGTGATATCTGCAAGGAGAGGCGTGCATATCTATTTTGCAAAGAAGATAGAGCAATACTTTGCAGGGAATGTGACATTCCTATCCATGAAGTCAACAAACTTACCAAGCAACACAACAGGTTTCTTCTCACCGGCGTAAAGATCGGTGCTTCTTCTTCTTATTCAAACCCAACAACTTCCAATGGCTCAGAACTAAGAACCTCAAGTCCAAGACCAAGTTCATTTTCAAGTGAAAATAATAGTTGTTCTCAAAGTTCTTTCAAAGAAAACATGGCTTGTGACACAGTTTCAACCAGTAGCATTTCCGAATACTTGATTGAAACAATTCCCGGTTACTGCATGGAAGACCTTTTTGATGCTTCTTTTACACCTAATAACGTTTTCTGTAAGGATTATCATGAACAGAACCAAGATCTTCAAGTTATAAACATGTCTGACTGGGTACCACAATCTCAAGTTAGATTCCCTCAGCTTAGCGCTAATTCGAATGTTCCCCACATTGATTCTTTAGACGGGGTTATGAAAATGCAACATACTAAAGTTGTTGAAGGATACAAAGTTCCTTCAATTAGTCCTCCATTCTTCAAACAATGTAGAGGCACTCGTTAA
Protein sequence

>Msa1339260

MKIQCDACHKQEASLFCPADEAALCNQCDRNIHYANKVSAKHKRFTLHHPTSKDTPLCDICKERRAYLFCKEDRAILCRECDIPIHEVNKLTKQHNRFLLTGVKIGASSSYSNPTTSNGSELRTSSPRPSSFSSENNSCSQSSFKENMACDTVSTSSISEYLIETIPGYCMEDLFDASFTPNNVFCKDYHEQNQDLQVINMSDWVPQSQVRFPQLSANSNVPHIDSLDGVMKMQHTKVVEGYKVPSISPPFFKQCRGTR*