AlfalfaGEDB | Msa0606150

M. sativa cultivar zhongmu-4 / Msa0606150

KEGG: -
GO: -
PFAM: PF00643.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0606150	XP_003607012.1	96.429	224	7	1	1	223	1	224	1.18e-157	447

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0606150	sp\|Q0IGM7\|BBX20_ARATH	53.000	200	70	4	1	176	1	200	4.91e-58	187

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0606150	G7JIM6	96.429	224	7	1	1	223	1	224	5.62e-158	447

TFs/TRs:

Gene ID	Type	Classification
Msa0606150	TF	DBB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0013270	Msa0606150	0.814109	1.821690e-51	-8.615850e-47
Msa0017710	Msa0606150	0.801056	1.077905e-48	-8.615850e-47
Msa0036810	Msa0606150	0.804535	2.061581e-49	-8.615850e-47
Msa0037180	Msa0606150	0.822003	2.988522e-53	-8.615850e-47
Msa0039750	Msa0606150	0.815552	8.719472e-52	-8.615850e-47
Msa0040860	Msa0606150	-0.821205	4.570306e-53	-8.615850e-47
Msa0045810	Msa0606150	0.812233	4.698679e-51	-8.615850e-47
Msa0046200	Msa0606150	0.801463	8.896485e-49	-8.615850e-47
Msa0046970	Msa0606150	0.824784	6.690727e-54	-8.615850e-47
Msa0092220	Msa0606150	0.807900	4.032321e-50	-8.615850e-47
Msa0106540	Msa0606150	0.808165	3.540903e-50	-8.615850e-47
Msa0606150	Msa0608820	0.841270	5.260547e-58	-8.615850e-47
Msa0606150	Msa0689830	0.819202	1.314810e-52	-8.615850e-47
Msa0606150	Msa0691610	0.837764	4.287902e-57	-8.615850e-47
Msa0606150	Msa0702690	-0.802258	6.110759e-49	-8.615850e-47
Msa0606150	Msa0704690	0.810186	1.306306e-50	-8.615850e-47
Msa0606150	Msa0732970	0.810483	1.126928e-50	-8.615850e-47
Msa0606150	Msa0745140	0.822430	2.378559e-53	-8.615850e-47
Msa0606150	Msa0761830	0.806592	7.633808e-50	-8.615850e-47
Msa0606150	Msa0769910	-0.800775	1.230018e-48	-8.615850e-47
Msa0606150	Msa0773960	0.817341	3.467673e-52	-8.615850e-47
Msa0606150	Msa0776640	0.822508	2.282304e-53	-8.615850e-47
Msa0606150	Msa0802950	-0.811965	5.375396e-51	-8.615850e-47
Msa0606150	Msa0804170	0.833719	4.536353e-56	-8.615850e-47
Msa0606150	Msa0807870	-0.810160	1.323645e-50	-8.615850e-47
Msa0606150	Msa0825920	0.828084	1.094405e-54	-8.615850e-47
Msa0606150	Msa0842180	0.814518	1.478874e-51	-8.615850e-47
Msa0606150	Msa0857270	0.806761	7.030937e-50	-8.615850e-47
Msa0606150	Msa0868610	0.808115	3.628968e-50	-8.615850e-47
Msa0606150	Msa0880910	0.805868	1.084262e-49	-8.615850e-47
Msa0606150	Msa0930770	0.817061	4.009198e-52	-8.615850e-47
Msa0606150	Msa0932140	0.815877	7.380130e-52	-8.615850e-47
Msa0606150	Msa0932150	0.811422	7.058750e-51	-8.615850e-47
Msa0606150	Msa0967780	0.810043	1.402366e-50	-8.615850e-47
Msa0606150	Msa0967900	-0.811183	7.951318e-51	-8.615850e-47
Msa0606150	Msa0981670	0.819912	9.053364e-53	-8.615850e-47
Msa0606150	Msa0982730	0.800455	1.429488e-48	-8.615850e-47
Msa0606150	Msa1013190	0.800410	1.459921e-48	-8.615850e-47
Msa0606150	Msa1024570	0.807140	5.844883e-50	-8.615850e-47
Msa0606150	Msa1032740	0.814753	1.312240e-51	-8.615850e-47
Msa0606150	Msa1124210	0.800271	1.558250e-48	-8.615850e-47
Msa0606150	Msa1143490	0.821468	3.972979e-53	-8.615850e-47
Msa0606150	Msa1165460	0.802317	5.941637e-49	-8.615850e-47
Msa0606150	Msa1165880	0.806428	8.265685e-50	-8.615850e-47
Msa0606150	Msa1191530	-0.801816	7.532439e-49	-8.615850e-47
Msa0606150	Msa1205270	-0.820740	5.847495e-53	-8.615850e-47
Msa0606150	Msa1206220	0.804852	1.770186e-49	-8.615850e-47
Msa0606150	Msa1217890	0.838207	3.298162e-57	-8.615850e-47
Msa0606150	Msa1220720	0.811062	8.446377e-51	-8.615850e-47
Msa0606150	Msa1230560	0.820202	7.770635e-53	-8.615850e-47
Msa0606150	Msa1265570	0.817507	3.181006e-52	-8.615850e-47
Msa0606150	Msa1266120	-0.813052	3.111746e-51	-8.615850e-47
Msa0606150	Msa1283980	0.800508	1.394430e-48	-8.615850e-47
Msa0606150	Msa1297220	-0.819382	1.195878e-52	-8.615850e-47
Msa0606150	Msa1311370	0.802477	5.508496e-49	-8.615850e-47
Msa0606150	Msa1322980	0.817987	2.479253e-52	-8.615850e-47
Msa0606150	Msa1323260	-0.825220	5.279939e-54	-8.615850e-47
Msa0606150	Msa1328280	0.848148	7.370734e-60	-8.615850e-47
Msa0606150	Msa1334670	0.816676	4.890969e-52	-8.615850e-47
Msa0606150	Msa1339260	0.957672	1.538559e-115	-8.615850e-47
Msa0606150	Msa1342360	-0.808800	2.592149e-50	-8.615850e-47
Msa0606150	Msa1342760	0.810288	1.241920e-50	-8.615850e-47
Msa0606150	Msa1343450	-0.810620	1.052876e-50	-8.615850e-47
Msa0606150	Msa1348860	-0.801553	8.527006e-49	-8.615850e-47
Msa0606150	Msa1365230	0.812675	3.763614e-51	-8.615850e-47
Msa0606150	Msa1366140	-0.806451	8.174792e-50	-8.615850e-47
Msa0606150	Msa1405830	0.806943	6.435663e-50	-8.615850e-47
Msa0606150	Msa1413020	0.834574	2.769699e-56	-8.615850e-47
Msa0606150	Msa1439920	0.808073	3.705423e-50	-8.615850e-47
Msa0606150	Msa1457770	0.807438	5.053996e-50	-8.615850e-47
Msa0121320	Msa0606150	0.801400	9.166590e-49	-8.615850e-47
Msa0132080	Msa0606150	-0.818235	2.179237e-52	-8.615850e-47
Msa0143590	Msa0606150	0.809379	1.948193e-50	-8.615850e-47
Msa0166180	Msa0606150	0.800343	1.506584e-48	-8.615850e-47
Msa0177320	Msa0606150	0.817362	3.430357e-52	-8.615850e-47
Msa0211460	Msa0606150	0.810380	1.186196e-50	-8.615850e-47
Msa0225070	Msa0606150	-0.817967	2.505851e-52	-8.615850e-47
Msa0234170	Msa0606150	0.814022	1.903647e-51	-8.615850e-47
Msa0380980	Msa0606150	0.806242	9.046330e-50	-8.615850e-47
Msa0386880	Msa0606150	0.814887	1.225197e-51	-8.615850e-47
Msa0388210	Msa0606150	0.826277	2.962888e-54	-8.615850e-47
Msa0403740	Msa0606150	-0.812804	3.525152e-51	-8.615850e-47
Msa0412580	Msa0606150	-0.807465	4.988343e-50	-8.615850e-47
Msa0419420	Msa0606150	-0.805359	1.386980e-49	-8.615850e-47
Msa0422820	Msa0606150	0.807090	5.991400e-50	-8.615850e-47
Msa0456450	Msa0606150	-0.810004	1.429713e-50	-8.615850e-47
Msa0457070	Msa0606150	-0.819805	9.578589e-53	-8.615850e-47
Msa0469060	Msa0606150	0.815955	7.089770e-52	-8.615850e-47
Msa0240590	Msa0606150	-0.818357	2.044616e-52	-8.615850e-47
Msa0256540	Msa0606150	0.809694	1.666990e-50	-8.615850e-47
Msa0266140	Msa0606150	-0.817799	2.734080e-52	-8.615850e-47
Msa0267450	Msa0606150	-0.816389	5.671452e-52	-8.615850e-47
Msa0267510	Msa0606150	-0.814414	1.559458e-51	-8.615850e-47
Msa0269220	Msa0606150	-0.802636	5.107178e-49	-8.615850e-47
Msa0279090	Msa0606150	-0.804590	2.008548e-49	-8.615850e-47
Msa0279810	Msa0606150	0.808626	2.823446e-50	-8.615850e-47
Msa0290800	Msa0606150	0.818085	2.356693e-52	-8.615850e-47
Msa0304060	Msa0606150	-0.810181	1.309591e-50	-8.615850e-47
Msa0307430	Msa0606150	0.813757	2.177728e-51	-8.615850e-47
Msa0316000	Msa0606150	0.823366	1.439661e-53	-8.615850e-47
Msa0319220	Msa0606150	0.805465	1.317579e-49	-8.615850e-47
Msa0328870	Msa0606150	0.821741	3.436211e-53	-8.615850e-47
Msa0340270	Msa0606150	0.805325	1.409938e-49	-8.615850e-47
Msa0349930	Msa0606150	0.819931	8.961885e-53	-8.615850e-47
Msa0364030	Msa0606150	-0.811316	7.440680e-51	-8.615850e-47
Msa0550730	Msa0606150	0.872594	2.821373e-67	-8.615850e-47
Msa0555280	Msa0606150	0.814022	1.903647e-51	-8.615850e-47
Msa0576180	Msa0606150	0.804658	1.943795e-49	-8.615850e-47
Msa0576320	Msa0606150	0.801522	8.654449e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0606150	MtrunA17_Chr4g0035651	96.429	224	7	1	1	223	1	224	1.08e-161	447
Msa0606150	MtrunA17_Chr3g0145061	59.624	213	69	6	1	198	1	211	4.62e-77	232
Msa0606150	MtrunA17_Chr1g0155811	47.926	217	99	4	1	208	1	212	2.23e-57	184
Msa0606150	MtrunA17_Chr3g0142221	47.191	178	58	3	1	177	1	143	2.47e-51	167
Msa0606150	MtrunA17_Chr2g0314671	50.794	126	58	3	1	126	1	122	4.26e-36	129
Msa0606150	MtrunA17_Chr4g0033641	50.000	110	54	1	1	110	1	109	1.22e-34	124
Msa0606150	MtrunA17_Chr2g0329511	48.182	110	56	1	1	110	1	109	7.28e-32	117
Msa0606150	MtrunA17_Chr5g0405151	37.324	142	78	4	1	135	1	138	4.06e-23	92.8
Msa0606150	MtrunA17_Chr1g0150471	43.299	97	45	1	5	101	6	92	7.68e-20	86.7
Msa0606150	MtrunA17_Chr3g0136701	39.175	97	49	1	5	101	6	92	7.90e-17	78.2
Msa0606150	MtrunA17_Chr4g0072871	40.860	93	45	1	5	97	22	104	1.68e-16	78.2
Msa0606150	MtrunA17_Chr7g0220721	35.043	117	63	2	5	121	26	129	2.14e-15	75.1
Msa0606150	MtrunA17_Chr5g0429341	36.082	97	52	1	1	97	1	87	4.76e-14	71.2
Msa0606150	MtrunA17_Chr3g0120711	35.052	97	52	2	1	97	1	86	1.65e-11	63.5
Msa0606150	MtrunA17_Chr7g0268691	32.653	98	55	2	5	102	20	106	7.58e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0606150	AT4G39070.1	53.000	200	70	4	1	176	1	200	4.99e-59	187
Msa0606150	AT1G75540.1	36.025	322	135	9	1	252	1	321	7.72e-55	179
Msa0606150	AT1G06040.2	46.715	137	66	2	1	137	1	130	7.82e-37	128
Msa0606150	AT1G78600.1	51.724	116	55	1	1	116	1	115	1.77e-36	131
Msa0606150	AT1G78600.2	42.529	174	91	3	1	173	1	166	2.74e-36	131
Msa0606150	AT1G06040.1	46.715	137	66	2	1	137	1	130	8.30e-36	128
Msa0606150	AT2G31380.1	51.327	113	54	1	1	113	1	112	1.27e-33	122
Msa0606150	AT4G10240.1	44.138	145	67	4	1	135	1	141	1.36e-30	112
Msa0606150	AT4G38960.1	43.810	105	55	2	1	105	1	101	1.72e-23	94.0
Msa0606150	AT4G38960.6	43.810	105	55	2	1	105	1	101	1.72e-23	94.0
Msa0606150	AT2G21320.1	43.810	105	55	2	1	105	1	101	3.69e-22	90.5
Msa0606150	AT2G24790.2	41.237	97	47	1	5	101	8	94	3.87e-19	83.6
Msa0606150	AT2G24790.1	41.237	97	47	1	5	101	8	94	1.25e-18	83.6
Msa0606150	AT5G24930.1	37.113	97	51	1	5	101	6	92	2.90e-16	77.8
Msa0606150	AT5G15850.1	32.520	123	70	2	5	127	12	121	8.56e-16	76.3
Msa0606150	AT4G38960.3	31.081	148	55	3	1	105	1	144	1.52e-15	73.9
Msa0606150	AT5G15840.2	33.333	123	70	2	5	127	20	130	5.88e-15	73.2
Msa0606150	AT5G15840.1	33.333	123	70	2	5	127	20	130	9.77e-15	73.2
Msa0606150	AT3G02380.1	36.559	93	49	1	5	97	16	98	9.78e-15	73.2
Msa0606150	AT2G47890.2	40.244	82	39	1	5	86	13	84	2.20e-12	65.1
Msa0606150	AT1G28050.1	35.644	101	55	1	2	102	6	96	2.34e-12	66.6
Msa0606150	AT2G47890.3	40.244	82	39	1	5	86	13	84	3.11e-12	65.5
Msa0606150	AT5G48250.1	34.694	98	53	2	1	97	1	88	4.54e-12	65.5
Msa0606150	AT2G47890.1	40.244	82	39	1	5	86	13	84	5.35e-12	65.1
Msa0606150	AT2G33500.2	34.000	100	56	1	3	102	10	99	8.71e-12	64.7
Msa0606150	AT2G33500.1	34.000	100	56	1	3	102	10	99	8.90e-12	64.7
Msa0606150	AT5G57660.1	34.646	127	60	4	5	122	22	134	1.11e-11	64.3
Msa0606150	AT3G07650.3	36.047	86	45	1	1	86	1	76	4.34e-11	62.8
Msa0606150	AT3G07650.2	36.047	86	45	1	1	86	1	76	4.34e-11	62.8
Msa0606150	AT3G07650.4	36.047	86	45	1	1	86	1	76	4.34e-11	62.8
Msa0606150	AT3G07650.1	36.047	86	45	1	1	86	1	76	4.34e-11	62.8

Find 86 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTTGGATCTGATGAATTA+TGG	0.164578	4_2:-52263126	None:intergenic
AATTGTTTCAATCAAGTATT+CGG	0.289976	4_2:-52264108	None:intergenic
GGATGGGGACAAAAGAATTA+TGG	0.300907	4_2:-52263255	None:intergenic
ACCTTACAGAAAACGTTATT+AGG	0.333966	4_2:-52264173	None:intergenic
ATCACAGAGAGGGGTGTCTT+TGG	0.337373	4_2:-52263486	None:intergenic
CACCTTTACTACATTAACTT+TGG	0.337392	4_2:+52263222	Msa0606150:five_prime_UTR
TGTGCTATTTCTACTACATT+TGG	0.337992	4_2:-52263142	None:intergenic
ATTTGGATCTGATGAATTAT+GGG	0.352913	4_2:-52263125	None:intergenic
CTTGAAAATGAACTTGGTCT+TGG	0.353565	4_2:-52264011	None:intergenic
AAGGTCTTCCATGCAGTAAC+CGG	0.360314	4_2:-52264132	None:intergenic
TGTTTATAACTTGAAGATCT+TGG	0.361700	4_2:-52264342	None:intergenic
CTGAGCCATTGGAAGTTGTT+GGG	0.365142	4_2:-52263971	None:intergenic
TGGTAAGTTTGTTGACTTCA+TGG	0.370579	4_2:-52263887	None:intergenic
TTGTTCTCATCTAAGGTAGA+AGG	0.378807	4_2:+52264633	Msa0606150:three_prime_UTR
AAGATAGAGCAATACTTTGC+AGG	0.379068	4_2:+52263847	Msa0606150:CDS
TACTTGATTGAAACAATTCC+CGG	0.387327	4_2:+52264113	Msa0606150:CDS
TCTGAGCCATTGGAAGTTGT+TGG	0.394596	4_2:-52263972	None:intergenic
CAGAGAGCTGCTTCATCTGC+AGG	0.413650	4_2:-52263383	None:intergenic
GAGAGAGTCAGAAAATAAAT+AGG	0.421018	4_2:+52262985	Msa0606150:five_prime_UTR
CAGAGAGAACAGCTAAGATT+TGG	0.425768	4_2:-52263073	None:intergenic
CAACATACTAAAGCTGTTGA+AGG	0.434663	4_2:+52264463	Msa0606150:CDS
CCCCACATTGATTCTTTAGA+CGG	0.435559	4_2:+52264427	Msa0606150:CDS
ATGGATAAGAAAACAAATGA+TGG	0.444047	4_2:-52264578	None:intergenic
GTGTAAAAGAAGCATCAAAA+AGG	0.446259	4_2:-52264151	None:intergenic
AAGTTATAAACATGTCTGAC+TGG	0.451524	4_2:+52264353	Msa0606150:CDS
AACTTACCAAGCAACACAAC+AGG	0.456566	4_2:+52263901	Msa0606150:CDS
TTGAAGGCAAAGTGGGGATG+GGG	0.458406	4_2:-52263270	None:intergenic
TGAACTTGGTCTTGGACTTG+AGG	0.462812	4_2:-52264003	None:intergenic
GTGTTTAGCAGAGACCTTGT+TGG	0.467471	4_2:-52263435	None:intergenic
CTTCTCACCGGCGTAAAGAT+CGG	0.467524	4_2:+52263927	Msa0606150:CDS
CAGAGACCTTGTTGGCATAG+TGG	0.468511	4_2:-52263427	None:intergenic
CCCACATTGATTCTTTAGAC+GGG	0.468902	4_2:+52264428	Msa0606150:CDS
ATAATTCGTACATATGAAAC+GGG	0.469720	4_2:-52264693	None:intergenic
GATAATTCGTACATATGAAA+CGG	0.470506	4_2:-52264694	None:intergenic
TTTGAAGGCAAAGTGGGGAT+GGG	0.471662	4_2:-52263271	None:intergenic
GATGGGGACAAAAGAATTAT+GGG	0.472346	4_2:-52263254	None:intergenic
CCTCTACATTGTTATAAGAA+TGG	0.477400	4_2:-52264511	None:intergenic
GAGAGAAGTACATAATTAAG+AGG	0.479528	4_2:-52264660	None:intergenic
CGTACATATGAAACGGGAAA+AGG	0.480675	4_2:-52264687	None:intergenic
CAAGTATTCGGAAATGCTAC+TGG	0.481122	4_2:-52264096	None:intergenic
TCTGATGAATTATGGGCGTT+TGG	0.486017	4_2:-52263118	None:intergenic
TTCCAAAGTTAATGTAGTAA+AGG	0.489803	4_2:-52263224	None:intergenic
GGTTCTTAGTTCTGAGCCAT+TGG	0.490954	4_2:-52263982	None:intergenic
AGAGGGGTGTCTTTGGAAGT+GGG	0.493908	4_2:-52263479	None:intergenic
GAGAGGGGTGTCTTTGGAAG+TGG	0.504462	4_2:-52263480	None:intergenic
GCCATTGATTTAAAATCGAT+CGG	0.523753	4_2:+52263190	Msa0606150:five_prime_UTR
TTCTCTTTCTATGAGTAAAG+AGG	0.529085	4_2:-52262953	None:intergenic
AAGAAACCTGTTGTGTTGCT+TGG	0.533532	4_2:-52263907	None:intergenic
AGATAGAGCAATACTTTGCA+GGG	0.534009	4_2:+52263848	Msa0606150:CDS
AAGAATGGAGGACTAATTGA+AGG	0.535956	4_2:-52264496	None:intergenic
AACAATTCCCGGTTACTGCA+TGG	0.543090	4_2:+52264124	Msa0606150:CDS
AAAATAAATAGGTCTCGTAA+TGG	0.547529	4_2:+52262996	Msa0606150:five_prime_UTR
TTCATCTGCAGGGCAGAACA+AGG	0.547884	4_2:-52263372	None:intergenic
TGGATAAGAAAACAAATGAT+GGG	0.550535	4_2:-52264577	None:intergenic
CACAACAGGTTTCTTCTCAC+CGG	0.551440	4_2:+52263915	Msa0606150:CDS
TCGAATTAGCGCTAAGCTGA+GGG	0.554345	4_2:-52264399	None:intergenic
AAGTTCTTTCAAAGAAAACA+TGG	0.562402	4_2:+52264055	Msa0606150:CDS
TCAAACCCAACAACTTCCAA+TGG	0.565279	4_2:+52263966	Msa0606150:CDS
AGTTTGTTGACTTCATGGAT+AGG	0.572223	4_2:-52263882	None:intergenic
AGGTCTTCCATGCAGTAACC+GGG	0.572681	4_2:-52264131	None:intergenic
GAGGGGTGTCTTTGGAAGTG+GGG	0.580604	4_2:-52263478	None:intergenic
GGGTGTCTTTGGAAGTGGGG+TGG	0.581849	4_2:-52263475	None:intergenic
TCTCTTTCTATGAGTAAAGA+GGG	0.582196	4_2:-52262952	None:intergenic
TTCGAATTAGCGCTAAGCTG+AGG	0.584714	4_2:-52264400	None:intergenic
AGAGAGCTGCTTCATCTGCA+GGG	0.589609	4_2:-52263382	None:intergenic
ATATGAAACGGGAAAAGGAA+AGG	0.590584	4_2:-52264682	None:intergenic
GGGAATCTAACTTGAGATTG+TGG	0.592001	4_2:-52264379	None:intergenic
CCCGTCTAAAGAATCAATGT+GGG	0.595981	4_2:-52264428	None:intergenic
TTACACGCATTAGTTGATCT+CGG	0.600288	4_2:+52263168	Msa0606150:five_prime_UTR
CAATATCCACTATGCCAACA+AGG	0.603596	4_2:+52263421	Msa0606150:CDS
CCACATTGATTCTTTAGACG+GGG	0.606501	4_2:+52264429	Msa0606150:CDS
CCTTGCAGATATCACAGAGA+GGG	0.606602	4_2:-52263496	None:intergenic
CCCTCTCTGTGATATCTGCA+AGG	0.608181	4_2:+52263496	Msa0606150:CDS
CCCCGTCTAAAGAATCAATG+TGG	0.617525	4_2:-52264429	None:intergenic
GTTTGTGACATGCATCACAC+TGG	0.618408	4_2:-52263340	None:intergenic
TGATGCATGTCACAAACAAG+AGG	0.622158	4_2:+52263346	Msa0606150:CDS
CTACATTGTTATAAGAATGG+AGG	0.631365	4_2:-52264508	None:intergenic
AGTTATAAACATGTCTGACT+GGG	0.637692	4_2:+52264354	Msa0606150:CDS
CCATTCTTATAACAATGTAG+AGG	0.669229	4_2:+52264511	Msa0606150:exon
TCTCATCTAAGGTAGAAGGT+AGG	0.670836	4_2:+52264637	Msa0606150:three_prime_UTR
CATAGAAAGAGAATCACACA+TGG	0.682829	4_2:+52262963	Msa0606150:five_prime_UTR
ATCTGCAGGGCAGAACAAGG+AGG	0.684576	4_2:-52263369	None:intergenic
CTTGCAGATATCACAGAGAG+GGG	0.687405	4_2:-52263495	None:intergenic
AGAAGCACCGATCTTTACGC+CGG	0.690841	4_2:-52263934	None:intergenic
CCGTCTAAAGAATCAATGTG+GGG	0.706719	4_2:-52264427	None:intergenic
ACCTTGCAGATATCACAGAG+AGG	0.718667	4_2:-52263497	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGTTGTTTATCAAAAAAAT+AGG	+	chr4_2:52263691-52263710	Msa0606150:intron	15.0%
!!!	ATAACATTTTTATATATTGC+AGG	+	chr4_2:52263797-52263816	Msa0606150:intron	15.0%
!!	CTACTCAATTAATTAAAATA+AGG	+	chr4_2:52264275-52264294	Msa0606150:intron	15.0%
!!	TTCTAGATAAAAATTGTAAA+TGG	-	chr4_2:52264600-52264619	None:intergenic	15.0%
!!	AAGTTGTTTATCAAAAAAAT+AGG	+	chr4_2:52263691-52263710	Msa0606150:intron	15.0%
!!!	ATAACATTTTTATATATTGC+AGG	+	chr4_2:52263797-52263816	Msa0606150:intron	15.0%
!!	CTACTCAATTAATTAAAATA+AGG	+	chr4_2:52264275-52264294	Msa0606150:intron	15.0%
!!	TTCTAGATAAAAATTGTAAA+TGG	-	chr4_2:52264600-52264619	None:intergenic	15.0%
!!!	AACTAATATGACCATATTTT+TGG	-	chr4_2:52263742-52263761	None:intergenic	20.0%
!!	AATTGTTTCAATCAAGTATT+CGG	-	chr4_2:52264111-52264130	None:intergenic	20.0%
!!!	AATAAGGTGTATATGTTTTT+TGG	+	chr4_2:52264291-52264310	Msa0606150:intron	20.0%
!!!	AACTAATATGACCATATTTT+TGG	-	chr4_2:52263742-52263761	None:intergenic	20.0%
!!	AATTGTTTCAATCAAGTATT+CGG	-	chr4_2:52264111-52264130	None:intergenic	20.0%
!!!	AATAAGGTGTATATGTTTTT+TGG	+	chr4_2:52264291-52264310	Msa0606150:intron	20.0%
!	AAAATAAATAGGTCTCGTAA+TGG	+	chr4_2:52262996-52263015	Msa0606150:five_prime_UTR	25.0%
!	ATCTGACTTTCAGAAAAATT+TGG	-	chr4_2:52263029-52263048	None:intergenic	25.0%
!	ATTTGGATCTGATGAATTAT+GGG	-	chr4_2:52263128-52263147	None:intergenic	25.0%
!	TTCCAAAGTTAATGTAGTAA+AGG	-	chr4_2:52263227-52263246	None:intergenic	25.0%
!	AATAAAAGACTGAGTGATAT+AGG	+	chr4_2:52263772-52263791	Msa0606150:intron	25.0%
!!	ATTTTTATATATTGCAGGAG+AGG	+	chr4_2:52263802-52263821	Msa0606150:intron	25.0%
!!	TTTTCACTTGAAAATGAACT+TGG	-	chr4_2:52264020-52264039	None:intergenic	25.0%
!	AAGTTCTTTCAAAGAAAACA+TGG	+	chr4_2:52264055-52264074	Msa0606150:CDS	25.0%
!!!	ATGTTTTTTGGTATTGTTTC+AGG	+	chr4_2:52264303-52264322	Msa0606150:intron	25.0%
!	TGTTTATAACTTGAAGATCT+TGG	-	chr4_2:52264345-52264364	None:intergenic	25.0%
!	TGGATAAGAAAACAAATGAT+GGG	-	chr4_2:52264580-52264599	None:intergenic	25.0%
!	ATGGATAAGAAAACAAATGA+TGG	-	chr4_2:52264581-52264600	None:intergenic	25.0%
!	AAAATAAATAGGTCTCGTAA+TGG	+	chr4_2:52262996-52263015	Msa0606150:five_prime_UTR	25.0%
!	ATCTGACTTTCAGAAAAATT+TGG	-	chr4_2:52263029-52263048	None:intergenic	25.0%
!	ATTTGGATCTGATGAATTAT+GGG	-	chr4_2:52263128-52263147	None:intergenic	25.0%
!	TTCCAAAGTTAATGTAGTAA+AGG	-	chr4_2:52263227-52263246	None:intergenic	25.0%
!	AATAAAAGACTGAGTGATAT+AGG	+	chr4_2:52263772-52263791	Msa0606150:intron	25.0%
!!	ATTTTTATATATTGCAGGAG+AGG	+	chr4_2:52263802-52263821	Msa0606150:intron	25.0%
!!	TTTTCACTTGAAAATGAACT+TGG	-	chr4_2:52264020-52264039	None:intergenic	25.0%
!	AAGTTCTTTCAAAGAAAACA+TGG	+	chr4_2:52264055-52264074	Msa0606150:CDS	25.0%
!!!	ATGTTTTTTGGTATTGTTTC+AGG	+	chr4_2:52264303-52264322	Msa0606150:intron	25.0%
!	TGTTTATAACTTGAAGATCT+TGG	-	chr4_2:52264345-52264364	None:intergenic	25.0%
!	TGGATAAGAAAACAAATGAT+GGG	-	chr4_2:52264580-52264599	None:intergenic	25.0%
!	ATGGATAAGAAAACAAATGA+TGG	-	chr4_2:52264581-52264600	None:intergenic	25.0%
	TCTCTTTCTATGAGTAAAGA+GGG	-	chr4_2:52262955-52262974	None:intergenic	30.0%
	TTCTCTTTCTATGAGTAAAG+AGG	-	chr4_2:52262956-52262975	None:intergenic	30.0%
	GAGAGAGTCAGAAAATAAAT+AGG	+	chr4_2:52262985-52263004	Msa0606150:five_prime_UTR	30.0%
	CATTTGGATCTGATGAATTA+TGG	-	chr4_2:52263129-52263148	None:intergenic	30.0%
!!	TGTGCTATTTCTACTACATT+TGG	-	chr4_2:52263145-52263164	None:intergenic	30.0%
!!	GCCATTGATTTAAAATCGAT+CGG	+	chr4_2:52263190-52263209	Msa0606150:five_prime_UTR	30.0%
	CACCTTTACTACATTAACTT+TGG	+	chr4_2:52263222-52263241	Msa0606150:five_prime_UTR	30.0%
!!!	TTTTGTTTTGAAGGCAAAGT+GGG	-	chr4_2:52263280-52263299	None:intergenic	30.0%
!!!	TTTTTGTTTTGAAGGCAAAG+TGG	-	chr4_2:52263281-52263300	None:intergenic	30.0%
!!!	AGGTTTGGTTTTTGTTTTGA+AGG	-	chr4_2:52263289-52263308	None:intergenic	30.0%
	AAGTACGTAAGAAATAGAGA+AGG	-	chr4_2:52263528-52263547	None:intergenic	30.0%
!	TTTCTTACGTACTTTTCAAC+AGG	+	chr4_2:52263534-52263553	Msa0606150:intron	30.0%
!	TTCTTACGTACTTTTCAACA+GGG	+	chr4_2:52263535-52263554	Msa0606150:intron	30.0%
	CGCTGAAAATCAGTTAAATA+AGG	-	chr4_2:52263643-52263662	None:intergenic	30.0%
!!!	ACCATATTTTTGGATCGTAT+AGG	-	chr4_2:52263732-52263751	None:intergenic	30.0%
	TACTTGATTGAAACAATTCC+CGG	+	chr4_2:52264113-52264132	Msa0606150:CDS	30.0%
!!	GTGTAAAAGAAGCATCAAAA+AGG	-	chr4_2:52264154-52264173	None:intergenic	30.0%
!	ACCTAATAACGTTTTCTGTA+AGG	+	chr4_2:52264172-52264191	Msa0606150:CDS	30.0%
	ACCTTACAGAAAACGTTATT+AGG	-	chr4_2:52264176-52264195	None:intergenic	30.0%
!!	AACGTTTTCTGTAAGGTATT+TGG	+	chr4_2:52264179-52264198	Msa0606150:intron	30.0%
!	AAAAGAAAAAAGTGCACTGT+TGG	-	chr4_2:52264208-52264227	None:intergenic	30.0%
	TAGATGAAAAATAGCTAGCT+AGG	-	chr4_2:52264258-52264277	None:intergenic	30.0%
	AAGTTATAAACATGTCTGAC+TGG	+	chr4_2:52264353-52264372	Msa0606150:CDS	30.0%
	AGTTATAAACATGTCTGACT+GGG	+	chr4_2:52264354-52264373	Msa0606150:CDS	30.0%
	CTACATTGTTATAAGAATGG+AGG	-	chr4_2:52264511-52264530	None:intergenic	30.0%
	CCTCTACATTGTTATAAGAA+TGG	-	chr4_2:52264514-52264533	None:intergenic	30.0%
	CCATTCTTATAACAATGTAG+AGG	+	chr4_2:52264511-52264530	Msa0606150:exon	30.0%
!	TGCAATTTTGTTCTCATCTA+AGG	+	chr4_2:52264626-52264645	Msa0606150:three_prime_UTR	30.0%
!	GAGAGAAGTACATAATTAAG+AGG	-	chr4_2:52264663-52264682	None:intergenic	30.0%
	TCTCTTTCTATGAGTAAAGA+GGG	-	chr4_2:52262955-52262974	None:intergenic	30.0%
	TTCTCTTTCTATGAGTAAAG+AGG	-	chr4_2:52262956-52262975	None:intergenic	30.0%
	GAGAGAGTCAGAAAATAAAT+AGG	+	chr4_2:52262985-52263004	Msa0606150:five_prime_UTR	30.0%
	CATTTGGATCTGATGAATTA+TGG	-	chr4_2:52263129-52263148	None:intergenic	30.0%
!!	TGTGCTATTTCTACTACATT+TGG	-	chr4_2:52263145-52263164	None:intergenic	30.0%
!!	GCCATTGATTTAAAATCGAT+CGG	+	chr4_2:52263190-52263209	Msa0606150:five_prime_UTR	30.0%
	CACCTTTACTACATTAACTT+TGG	+	chr4_2:52263222-52263241	Msa0606150:five_prime_UTR	30.0%
!!!	TTTTGTTTTGAAGGCAAAGT+GGG	-	chr4_2:52263280-52263299	None:intergenic	30.0%
!!!	TTTTTGTTTTGAAGGCAAAG+TGG	-	chr4_2:52263281-52263300	None:intergenic	30.0%
!!!	AGGTTTGGTTTTTGTTTTGA+AGG	-	chr4_2:52263289-52263308	None:intergenic	30.0%
	AAGTACGTAAGAAATAGAGA+AGG	-	chr4_2:52263528-52263547	None:intergenic	30.0%
!	TTTCTTACGTACTTTTCAAC+AGG	+	chr4_2:52263534-52263553	Msa0606150:intron	30.0%
!	TTCTTACGTACTTTTCAACA+GGG	+	chr4_2:52263535-52263554	Msa0606150:intron	30.0%
	CGCTGAAAATCAGTTAAATA+AGG	-	chr4_2:52263643-52263662	None:intergenic	30.0%
!!!	ACCATATTTTTGGATCGTAT+AGG	-	chr4_2:52263732-52263751	None:intergenic	30.0%
	TACTTGATTGAAACAATTCC+CGG	+	chr4_2:52264113-52264132	Msa0606150:CDS	30.0%
!!	GTGTAAAAGAAGCATCAAAA+AGG	-	chr4_2:52264154-52264173	None:intergenic	30.0%
!	ACCTAATAACGTTTTCTGTA+AGG	+	chr4_2:52264172-52264191	Msa0606150:CDS	30.0%
	ACCTTACAGAAAACGTTATT+AGG	-	chr4_2:52264176-52264195	None:intergenic	30.0%
!!	AACGTTTTCTGTAAGGTATT+TGG	+	chr4_2:52264179-52264198	Msa0606150:intron	30.0%
!	AAAAGAAAAAAGTGCACTGT+TGG	-	chr4_2:52264208-52264227	None:intergenic	30.0%
	TAGATGAAAAATAGCTAGCT+AGG	-	chr4_2:52264258-52264277	None:intergenic	30.0%
	AAGTTATAAACATGTCTGAC+TGG	+	chr4_2:52264353-52264372	Msa0606150:CDS	30.0%
	AGTTATAAACATGTCTGACT+GGG	+	chr4_2:52264354-52264373	Msa0606150:CDS	30.0%
	CTACATTGTTATAAGAATGG+AGG	-	chr4_2:52264511-52264530	None:intergenic	30.0%
	CCTCTACATTGTTATAAGAA+TGG	-	chr4_2:52264514-52264533	None:intergenic	30.0%
	CCATTCTTATAACAATGTAG+AGG	+	chr4_2:52264511-52264530	Msa0606150:exon	30.0%
!	TGCAATTTTGTTCTCATCTA+AGG	+	chr4_2:52264626-52264645	Msa0606150:three_prime_UTR	30.0%
!	GAGAGAAGTACATAATTAAG+AGG	-	chr4_2:52264663-52264682	None:intergenic	30.0%
	CATAGAAAGAGAATCACACA+TGG	+	chr4_2:52262963-52262982	Msa0606150:five_prime_UTR	35.0%
	TTACACGCATTAGTTGATCT+CGG	+	chr4_2:52263168-52263187	Msa0606150:five_prime_UTR	35.0%
!!	GCCGATCGATTTTAAATCAA+TGG	-	chr4_2:52263194-52263213	None:intergenic	35.0%
	GATGGGGACAAAAGAATTAT+GGG	-	chr4_2:52263257-52263276	None:intergenic	35.0%
!!!	TTTGTTTTGAAGGCAAAGTG+GGG	-	chr4_2:52263279-52263298	None:intergenic	35.0%
!!!	TCTATTTTTGAGCGTTTTGG+AGG	-	chr4_2:52263309-52263328	None:intergenic	35.0%
!!!	CTCTCTATTTTTGAGCGTTT+TGG	-	chr4_2:52263312-52263331	None:intergenic	35.0%
	GCCTATACGATCCAAAAATA+TGG	+	chr4_2:52263728-52263747	Msa0606150:intron	35.0%
	AAGATAGAGCAATACTTTGC+AGG	+	chr4_2:52263847-52263866	Msa0606150:CDS	35.0%
	AGATAGAGCAATACTTTGCA+GGG	+	chr4_2:52263848-52263867	Msa0606150:CDS	35.0%
!!	AGTTTGTTGACTTCATGGAT+AGG	-	chr4_2:52263885-52263904	None:intergenic	35.0%
!!	TGGTAAGTTTGTTGACTTCA+TGG	-	chr4_2:52263890-52263909	None:intergenic	35.0%
	CTTGAAAATGAACTTGGTCT+TGG	-	chr4_2:52264014-52264033	None:intergenic	35.0%
	CAACATACTAAAGCTGTTGA+AGG	+	chr4_2:52264463-52264482	Msa0606150:CDS	35.0%
	AAGAATGGAGGACTAATTGA+AGG	-	chr4_2:52264499-52264518	None:intergenic	35.0%
!	TTGTTCTCATCTAAGGTAGA+AGG	+	chr4_2:52264633-52264652	Msa0606150:three_prime_UTR	35.0%
	ATATGAAACGGGAAAAGGAA+AGG	-	chr4_2:52264685-52264704	None:intergenic	35.0%
	CATAGAAAGAGAATCACACA+TGG	+	chr4_2:52262963-52262982	Msa0606150:five_prime_UTR	35.0%
	TTACACGCATTAGTTGATCT+CGG	+	chr4_2:52263168-52263187	Msa0606150:five_prime_UTR	35.0%
!!	GCCGATCGATTTTAAATCAA+TGG	-	chr4_2:52263194-52263213	None:intergenic	35.0%
	GATGGGGACAAAAGAATTAT+GGG	-	chr4_2:52263257-52263276	None:intergenic	35.0%
!!!	TTTGTTTTGAAGGCAAAGTG+GGG	-	chr4_2:52263279-52263298	None:intergenic	35.0%
!!!	TCTATTTTTGAGCGTTTTGG+AGG	-	chr4_2:52263309-52263328	None:intergenic	35.0%
!!!	CTCTCTATTTTTGAGCGTTT+TGG	-	chr4_2:52263312-52263331	None:intergenic	35.0%
	GCCTATACGATCCAAAAATA+TGG	+	chr4_2:52263728-52263747	Msa0606150:intron	35.0%
	AAGATAGAGCAATACTTTGC+AGG	+	chr4_2:52263847-52263866	Msa0606150:CDS	35.0%
	AGATAGAGCAATACTTTGCA+GGG	+	chr4_2:52263848-52263867	Msa0606150:CDS	35.0%
!!	AGTTTGTTGACTTCATGGAT+AGG	-	chr4_2:52263885-52263904	None:intergenic	35.0%
!!	TGGTAAGTTTGTTGACTTCA+TGG	-	chr4_2:52263890-52263909	None:intergenic	35.0%
	CTTGAAAATGAACTTGGTCT+TGG	-	chr4_2:52264014-52264033	None:intergenic	35.0%
	CAACATACTAAAGCTGTTGA+AGG	+	chr4_2:52264463-52264482	Msa0606150:CDS	35.0%
	AAGAATGGAGGACTAATTGA+AGG	-	chr4_2:52264499-52264518	None:intergenic	35.0%
!	TTGTTCTCATCTAAGGTAGA+AGG	+	chr4_2:52264633-52264652	Msa0606150:three_prime_UTR	35.0%
	ATATGAAACGGGAAAAGGAA+AGG	-	chr4_2:52264685-52264704	None:intergenic	35.0%
	CAGAGAGAACAGCTAAGATT+TGG	-	chr4_2:52263076-52263095	None:intergenic	40.0%
	TCTGATGAATTATGGGCGTT+TGG	-	chr4_2:52263121-52263140	None:intergenic	40.0%
	GGATGGGGACAAAAGAATTA+TGG	-	chr4_2:52263258-52263277	None:intergenic	40.0%
!!!	TTTTGAGCGTTTTGGAGGTT+TGG	-	chr4_2:52263304-52263323	None:intergenic	40.0%
	TGATGCATGTCACAAACAAG+AGG	+	chr4_2:52263346-52263365	Msa0606150:CDS	40.0%
	CAATATCCACTATGCCAACA+AGG	+	chr4_2:52263421-52263440	Msa0606150:CDS	40.0%
	AACTTACCAAGCAACACAAC+AGG	+	chr4_2:52263901-52263920	Msa0606150:CDS	40.0%
!	AAGAAACCTGTTGTGTTGCT+TGG	-	chr4_2:52263910-52263929	None:intergenic	40.0%
	TCAAACCCAACAACTTCCAA+TGG	+	chr4_2:52263966-52263985	Msa0606150:CDS	40.0%
	CAAGTATTCGGAAATGCTAC+TGG	-	chr4_2:52264099-52264118	None:intergenic	40.0%
	GGGAATCTAACTTGAGATTG+TGG	-	chr4_2:52264382-52264401	None:intergenic	40.0%
	CCGTCTAAAGAATCAATGTG+GGG	-	chr4_2:52264430-52264449	None:intergenic	40.0%
!!	CCCCACATTGATTCTTTAGA+CGG	+	chr4_2:52264427-52264446	Msa0606150:CDS	40.0%
	CCCGTCTAAAGAATCAATGT+GGG	-	chr4_2:52264431-52264450	None:intergenic	40.0%
!!	CCCACATTGATTCTTTAGAC+GGG	+	chr4_2:52264428-52264447	Msa0606150:CDS	40.0%
!!	CCACATTGATTCTTTAGACG+GGG	+	chr4_2:52264429-52264448	Msa0606150:CDS	40.0%
!	TCTCATCTAAGGTAGAAGGT+AGG	+	chr4_2:52264637-52264656	Msa0606150:three_prime_UTR	40.0%
	CGTACATATGAAACGGGAAA+AGG	-	chr4_2:52264690-52264709	None:intergenic	40.0%
	CAGAGAGAACAGCTAAGATT+TGG	-	chr4_2:52263076-52263095	None:intergenic	40.0%
	TCTGATGAATTATGGGCGTT+TGG	-	chr4_2:52263121-52263140	None:intergenic	40.0%
	GGATGGGGACAAAAGAATTA+TGG	-	chr4_2:52263258-52263277	None:intergenic	40.0%
!!!	TTTTGAGCGTTTTGGAGGTT+TGG	-	chr4_2:52263304-52263323	None:intergenic	40.0%
	TGATGCATGTCACAAACAAG+AGG	+	chr4_2:52263346-52263365	Msa0606150:CDS	40.0%
	CAATATCCACTATGCCAACA+AGG	+	chr4_2:52263421-52263440	Msa0606150:CDS	40.0%
	AACTTACCAAGCAACACAAC+AGG	+	chr4_2:52263901-52263920	Msa0606150:CDS	40.0%
!	AAGAAACCTGTTGTGTTGCT+TGG	-	chr4_2:52263910-52263929	None:intergenic	40.0%
	TCAAACCCAACAACTTCCAA+TGG	+	chr4_2:52263966-52263985	Msa0606150:CDS	40.0%
	CAAGTATTCGGAAATGCTAC+TGG	-	chr4_2:52264099-52264118	None:intergenic	40.0%
	GGGAATCTAACTTGAGATTG+TGG	-	chr4_2:52264382-52264401	None:intergenic	40.0%
	CCGTCTAAAGAATCAATGTG+GGG	-	chr4_2:52264430-52264449	None:intergenic	40.0%
!!	CCCCACATTGATTCTTTAGA+CGG	+	chr4_2:52264427-52264446	Msa0606150:CDS	40.0%
	CCCGTCTAAAGAATCAATGT+GGG	-	chr4_2:52264431-52264450	None:intergenic	40.0%
!!	CCCACATTGATTCTTTAGAC+GGG	+	chr4_2:52264428-52264447	Msa0606150:CDS	40.0%
!!	CCACATTGATTCTTTAGACG+GGG	+	chr4_2:52264429-52264448	Msa0606150:CDS	40.0%
!	TCTCATCTAAGGTAGAAGGT+AGG	+	chr4_2:52264637-52264656	Msa0606150:three_prime_UTR	40.0%
	CGTACATATGAAACGGGAAA+AGG	-	chr4_2:52264690-52264709	None:intergenic	40.0%
	TTTGAAGGCAAAGTGGGGAT+GGG	-	chr4_2:52263274-52263293	None:intergenic	45.0%
!!	TTTTGAAGGCAAAGTGGGGA+TGG	-	chr4_2:52263275-52263294	None:intergenic	45.0%
	GTTTGTGACATGCATCACAC+TGG	-	chr4_2:52263343-52263362	None:intergenic	45.0%
	GTGTTTAGCAGAGACCTTGT+TGG	-	chr4_2:52263438-52263457	None:intergenic	45.0%
	CTTGCAGATATCACAGAGAG+GGG	-	chr4_2:52263498-52263517	None:intergenic	45.0%
	CCTTGCAGATATCACAGAGA+GGG	-	chr4_2:52263499-52263518	None:intergenic	45.0%
	ACCTTGCAGATATCACAGAG+AGG	-	chr4_2:52263500-52263519	None:intergenic	45.0%
	CACAACAGGTTTCTTCTCAC+CGG	+	chr4_2:52263915-52263934	Msa0606150:CDS	45.0%
	CTGAGCCATTGGAAGTTGTT+GGG	-	chr4_2:52263974-52263993	None:intergenic	45.0%
	TCTGAGCCATTGGAAGTTGT+TGG	-	chr4_2:52263975-52263994	None:intergenic	45.0%
!!	GGTTCTTAGTTCTGAGCCAT+TGG	-	chr4_2:52263985-52264004	None:intergenic	45.0%
!!	TGAACTTGGTCTTGGACTTG+AGG	-	chr4_2:52264006-52264025	None:intergenic	45.0%
	AACAATTCCCGGTTACTGCA+TGG	+	chr4_2:52264124-52264143	Msa0606150:CDS	45.0%
	AAGGTCTTCCATGCAGTAAC+CGG	-	chr4_2:52264135-52264154	None:intergenic	45.0%
	TCGAATTAGCGCTAAGCTGA+GGG	-	chr4_2:52264402-52264421	None:intergenic	45.0%
	TTCGAATTAGCGCTAAGCTG+AGG	-	chr4_2:52264403-52264422	None:intergenic	45.0%
	CCCCGTCTAAAGAATCAATG+TGG	-	chr4_2:52264432-52264451	None:intergenic	45.0%
	TTTGAAGGCAAAGTGGGGAT+GGG	-	chr4_2:52263274-52263293	None:intergenic	45.0%
!!	TTTTGAAGGCAAAGTGGGGA+TGG	-	chr4_2:52263275-52263294	None:intergenic	45.0%
	GTTTGTGACATGCATCACAC+TGG	-	chr4_2:52263343-52263362	None:intergenic	45.0%
	GTGTTTAGCAGAGACCTTGT+TGG	-	chr4_2:52263438-52263457	None:intergenic	45.0%
	CTTGCAGATATCACAGAGAG+GGG	-	chr4_2:52263498-52263517	None:intergenic	45.0%
	CCTTGCAGATATCACAGAGA+GGG	-	chr4_2:52263499-52263518	None:intergenic	45.0%
	ACCTTGCAGATATCACAGAG+AGG	-	chr4_2:52263500-52263519	None:intergenic	45.0%
	CACAACAGGTTTCTTCTCAC+CGG	+	chr4_2:52263915-52263934	Msa0606150:CDS	45.0%
	CTGAGCCATTGGAAGTTGTT+GGG	-	chr4_2:52263974-52263993	None:intergenic	45.0%
	TCTGAGCCATTGGAAGTTGT+TGG	-	chr4_2:52263975-52263994	None:intergenic	45.0%
!!	GGTTCTTAGTTCTGAGCCAT+TGG	-	chr4_2:52263985-52264004	None:intergenic	45.0%
!!	TGAACTTGGTCTTGGACTTG+AGG	-	chr4_2:52264006-52264025	None:intergenic	45.0%
	AACAATTCCCGGTTACTGCA+TGG	+	chr4_2:52264124-52264143	Msa0606150:CDS	45.0%
	AAGGTCTTCCATGCAGTAAC+CGG	-	chr4_2:52264135-52264154	None:intergenic	45.0%
	TCGAATTAGCGCTAAGCTGA+GGG	-	chr4_2:52264402-52264421	None:intergenic	45.0%
	TTCGAATTAGCGCTAAGCTG+AGG	-	chr4_2:52264403-52264422	None:intergenic	45.0%
	CCCCGTCTAAAGAATCAATG+TGG	-	chr4_2:52264432-52264451	None:intergenic	45.0%
	TTGAAGGCAAAGTGGGGATG+GGG	-	chr4_2:52263273-52263292	None:intergenic	50.0%
	TTCATCTGCAGGGCAGAACA+AGG	-	chr4_2:52263375-52263394	None:intergenic	50.0%
	AGAGAGCTGCTTCATCTGCA+GGG	-	chr4_2:52263385-52263404	None:intergenic	50.0%
	CAGAGACCTTGTTGGCATAG+TGG	-	chr4_2:52263430-52263449	None:intergenic	50.0%
!!	AGAGGGGTGTCTTTGGAAGT+GGG	-	chr4_2:52263482-52263501	None:intergenic	50.0%
!!	ATCACAGAGAGGGGTGTCTT+TGG	-	chr4_2:52263489-52263508	None:intergenic	50.0%
	CCCTCTCTGTGATATCTGCA+AGG	+	chr4_2:52263496-52263515	Msa0606150:CDS	50.0%
	CTTCTCACCGGCGTAAAGAT+CGG	+	chr4_2:52263927-52263946	Msa0606150:CDS	50.0%
!!	AGAAGCACCGATCTTTACGC+CGG	-	chr4_2:52263937-52263956	None:intergenic	50.0%
	AGGTCTTCCATGCAGTAACC+GGG	-	chr4_2:52264134-52264153	None:intergenic	50.0%
	TTGAAGGCAAAGTGGGGATG+GGG	-	chr4_2:52263273-52263292	None:intergenic	50.0%
	TTCATCTGCAGGGCAGAACA+AGG	-	chr4_2:52263375-52263394	None:intergenic	50.0%
	AGAGAGCTGCTTCATCTGCA+GGG	-	chr4_2:52263385-52263404	None:intergenic	50.0%
	CAGAGACCTTGTTGGCATAG+TGG	-	chr4_2:52263430-52263449	None:intergenic	50.0%
!!	AGAGGGGTGTCTTTGGAAGT+GGG	-	chr4_2:52263482-52263501	None:intergenic	50.0%
!!	ATCACAGAGAGGGGTGTCTT+TGG	-	chr4_2:52263489-52263508	None:intergenic	50.0%
	CCCTCTCTGTGATATCTGCA+AGG	+	chr4_2:52263496-52263515	Msa0606150:CDS	50.0%
	CTTCTCACCGGCGTAAAGAT+CGG	+	chr4_2:52263927-52263946	Msa0606150:CDS	50.0%
!!	AGAAGCACCGATCTTTACGC+CGG	-	chr4_2:52263937-52263956	None:intergenic	50.0%
	AGGTCTTCCATGCAGTAACC+GGG	-	chr4_2:52264134-52264153	None:intergenic	50.0%
	ATCTGCAGGGCAGAACAAGG+AGG	-	chr4_2:52263372-52263391	None:intergenic	55.0%
	CAGAGAGCTGCTTCATCTGC+AGG	-	chr4_2:52263386-52263405	None:intergenic	55.0%
!!	GAGGGGTGTCTTTGGAAGTG+GGG	-	chr4_2:52263481-52263500	None:intergenic	55.0%
!!	GAGAGGGGTGTCTTTGGAAG+TGG	-	chr4_2:52263483-52263502	None:intergenic	55.0%
	CTGCAACACAACCGCAGTAG+AGG	+	chr4_2:52263608-52263627	Msa0606150:intron	55.0%
	GGCTGATACTGCCTCTACTG+CGG	-	chr4_2:52263622-52263641	None:intergenic	55.0%
	ATCTGCAGGGCAGAACAAGG+AGG	-	chr4_2:52263372-52263391	None:intergenic	55.0%
	CAGAGAGCTGCTTCATCTGC+AGG	-	chr4_2:52263386-52263405	None:intergenic	55.0%
!!	GAGGGGTGTCTTTGGAAGTG+GGG	-	chr4_2:52263481-52263500	None:intergenic	55.0%
!!	GAGAGGGGTGTCTTTGGAAG+TGG	-	chr4_2:52263483-52263502	None:intergenic	55.0%
	CTGCAACACAACCGCAGTAG+AGG	+	chr4_2:52263608-52263627	Msa0606150:intron	55.0%
	GGCTGATACTGCCTCTACTG+CGG	-	chr4_2:52263622-52263641	None:intergenic	55.0%
!	GGGTGTCTTTGGAAGTGGGG+TGG	-	chr4_2:52263478-52263497	None:intergenic	60.0%
!	GGGTGTCTTTGGAAGTGGGG+TGG	-	chr4_2:52263478-52263497	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	52262952	52264713	52262952	ID=Msa0606150;Name=Msa0606150
chr4_2	mRNA	52262952	52264713	52262952	ID=Msa0606150-mRNA-1;Parent=Msa0606150;Name=Msa0606150-mRNA-1;_AED=0.05;_eAED=0.05;_QI=380\|1\|1\|1\|1\|1\|3\|191\|252
chr4_2	exon	52262952	52263517	52262952	ID=Msa0606150-mRNA-1:exon:12668;Parent=Msa0606150-mRNA-1
chr4_2	exon	52263819	52264193	52263819	ID=Msa0606150-mRNA-1:exon:12669;Parent=Msa0606150-mRNA-1
chr4_2	exon	52264325	52264713	52264325	ID=Msa0606150-mRNA-1:exon:12670;Parent=Msa0606150-mRNA-1
chr4_2	five_prime_UTR	52262952	52263331	52262952	ID=Msa0606150-mRNA-1:five_prime_utr;Parent=Msa0606150-mRNA-1
chr4_2	CDS	52263332	52263517	52263332	ID=Msa0606150-mRNA-1:cds;Parent=Msa0606150-mRNA-1
chr4_2	CDS	52263819	52264193	52263819	ID=Msa0606150-mRNA-1:cds;Parent=Msa0606150-mRNA-1
chr4_2	CDS	52264325	52264522	52264325	ID=Msa0606150-mRNA-1:cds;Parent=Msa0606150-mRNA-1
chr4_2	three_prime_UTR	52264523	52264713	52264523	ID=Msa0606150-mRNA-1:three_prime_utr;Parent=Msa0606150-mRNA-1

Gene Sequence

>Msa0606150

ATGAAGATCCAGTGTGATGCATGTCACAAACAAGAGGCCTCCTTGTTCTGCCCTGCAGATGAAGCAGCTCTCTGCAATCAATGTGATCGCAATATCCACTATGCCAACAAGGTCTCTGCTAAACACAAACGCTTCACTCTTCACCACCCCACTTCCAAAGACACCCCTCTCTGTGATATCTGCAAGGAGAGGCGTGCATATCTATTTTGCAAAGAAGATAGAGCAATACTTTGCAGGGAATGTGACATTCCTATCCATGAAGTCAACAAACTTACCAAGCAACACAACAGGTTTCTTCTCACCGGCGTAAAGATCGGTGCTTCTTCTTCTTATTCAAACCCAACAACTTCCAATGGCTCAGAACTAAGAACCTCAAGTCCAAGACCAAGTTCATTTTCAAGTGAAAATAATAGTTGTTCTCAAAGTTCTTTCAAAGAAAACATGGCTTGTGACACAGTTTCAACCAGTAGCATTTCCGAATACTTGATTGAAACAATTCCCGGTTACTGCATGGAAGACCTTTTTGATGCTTCTTTTACACCTAATAACGTTTTCTGTAAGGATTATCATGAACAGAACCAAGATCTTCAAGTTATAAACATGTCTGACTGGGTACCACAATCTCAAGTTAGATTCCCTCAGCTTAGCGCTAATTCGAATGTTCCCCACATTGATTCTTTAGACGGGGTTATGAAAATGCAACATACTAAAGCTGTTGAAGGATACAAAGTTCCTTCAATTAGTCCTCCATTCTTATAA

Protein sequence

>Msa0606150

MKIQCDACHKQEASLFCPADEAALCNQCDRNIHYANKVSAKHKRFTLHHPTSKDTPLCDICKERRAYLFCKEDRAILCRECDIPIHEVNKLTKQHNRFLLTGVKIGASSSYSNPTTSNGSELRTSSPRPSSFSSENNSCSQSSFKENMACDTVSTSSISEYLIETIPGYCMEDLFDASFTPNNVFCKDYHEQNQDLQVINMSDWVPQSQVRFPQLSANSNVPHIDSLDGVMKMQHTKAVEGYKVPSISPPFL*