AlfalfaGEDB | Msa0550730

M. sativa cultivar zhongmu-4 / Msa0550730

KEGG: -
GO: -
PFAM: PF00643.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0550730	XP_003607012.1	95.982	224	8	1	1	223	1	224	1.00e-155	442

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0550730	sp\|Q0IGM7\|BBX20_ARATH	52.500	200	71	4	1	176	1	200	6.11e-57	185

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0550730	G7JIM6	95.982	224	8	1	1	223	1	224	4.80e-156	442

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TFs/TRs:

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Gene ID	Type	Classification
Msa0550730	TF	DBB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0422820	Msa0550730	0.822188	2.707560e-53	-8.615850e-47
Msa0550730	Msa0606150	0.872594	2.821373e-67	-8.615850e-47
Msa0550730	Msa0804170	0.814826	1.264359e-51	-8.615850e-47
Msa0550730	Msa1328280	0.804524	2.072742e-49	-8.615850e-47
Msa0550730	Msa1339260	0.850384	1.758175e-60	-8.615850e-47
Msa0550730	Msa1413020	0.810354	1.201894e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0550730	MtrunA17_Chr4g0035651	95.982	224	8	1	1	223	1	224	9.24e-160	442
Msa0550730	MtrunA17_Chr3g0145061	59.624	213	69	6	1	198	1	211	3.66e-76	230
Msa0550730	MtrunA17_Chr1g0155811	47.926	217	99	4	1	208	1	212	6.00e-58	186
Msa0550730	MtrunA17_Chr3g0142221	47.191	178	58	3	1	177	1	143	3.55e-51	166
Msa0550730	MtrunA17_Chr2g0314671	54.717	106	47	1	1	106	1	105	9.81e-36	129
Msa0550730	MtrunA17_Chr4g0033641	50.000	110	54	1	1	110	1	109	5.29e-34	123
Msa0550730	MtrunA17_Chr2g0329511	49.057	106	53	1	1	106	1	105	2.67e-31	115
Msa0550730	MtrunA17_Chr5g0405151	44.000	100	52	2	1	100	1	96	1.45e-22	91.7
Msa0550730	MtrunA17_Chr1g0150471	43.299	97	45	1	5	101	6	92	1.11e-19	86.7
Msa0550730	MtrunA17_Chr3g0136701	39.175	97	49	1	5	101	6	92	1.25e-16	77.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0550730	AT4G39070.1	52.500	200	71	4	1	176	1	200	6.21e-58	185
Msa0550730	AT1G75540.1	35.938	320	134	9	1	250	1	319	6.20e-55	180
Msa0550730	AT1G06040.2	46.715	137	66	2	1	137	1	130	4.67e-36	126
Msa0550730	AT1G78600.1	42.529	174	91	3	1	173	1	166	1.05e-35	129
Msa0550730	AT1G78600.2	42.529	174	91	3	1	173	1	166	1.21e-35	129
Msa0550730	AT1G06040.1	46.715	137	66	2	1	137	1	130	3.59e-35	126
Msa0550730	AT2G31380.1	50.442	113	55	1	1	113	1	112	1.53e-32	119
Msa0550730	AT4G10240.1	45.000	140	56	4	1	135	1	124	4.76e-30	110
Msa0550730	AT4G38960.1	43.810	105	55	2	1	105	1	101	3.36e-23	93.6
Msa0550730	AT4G38960.6	43.810	105	55	2	1	105	1	101	3.36e-23	93.6

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Find 85 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAAATAGGTCTCGTAA+TGG	0.547529	4_1:+46254818	Msa0550730:five_prime_UTR
AACAATTCCTGGTTACTGCA+TGG	0.545989	4_1:+46255939	Msa0550730:CDS
AACTTACCAAGCAACACAAC+AGG	0.456566	4_1:+46255716	Msa0550730:CDS
AAGAAACCTGTTGTGTTGCT+TGG	0.533532	4_1:-46255722	None:intergenic
AAGAATGGAGGACTAATTGA+AGG	0.493324	4_1:-46256313	None:intergenic
AAGATAGAGCAATACTTTGC+AGG	0.379068	4_1:+46255662	Msa0550730:CDS
AAGTTATAAACATGTCTGAC+TGG	0.451524	4_1:+46256170	Msa0550730:CDS
AAGTTCTTTCAAAGAAAACA+TGG	0.562402	4_1:+46255870	Msa0550730:CDS
AATTGTTTCAATCAAGTATT+CGG	0.312961	4_1:-46255923	None:intergenic
ACAAGCACCGATCTTTACGC+CGG	0.707715	4_1:-46255749	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTCTTTCTATGAGTAAAGA+GGG	-	chr4_1:46254777-46254796	None:intergenic	30.0%
TTCTCTTTCTATGAGTAAAG+AGG	-	chr4_1:46254778-46254797	None:intergenic	30.0%
GAGAGAGTCAGAAAATAAAT+AGG	+	chr4_1:46254807-46254826	Msa0550730:five_prime_UTR	30.0%
CATTTGGATCTGATGAATTA+TGG	-	chr4_1:46254951-46254970	None:intergenic	30.0%
CACCTTTACTACATTAACTT+TGG	+	chr4_1:46255044-46255063	Msa0550730:five_prime_UTR	30.0%
GCCTATATGATCCAAAAATA+TGG	+	chr4_1:46255547-46255566	Msa0550730:intron	30.0%
TACTTGATTGAAACAATTCC+TGG	+	chr4_1:46255928-46255947	Msa0550730:CDS	30.0%
ACCTTACAGAAAACGTTATT+AGG	-	chr4_1:46255991-46256010	None:intergenic	30.0%
TAGATGAAAAATAGCTAGCT+AGG	-	chr4_1:46256075-46256094	None:intergenic	30.0%
AAGTTATAAACATGTCTGAC+TGG	+	chr4_1:46256170-46256189	Msa0550730:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	46254774	46256528	46254774	ID=Msa0550730;Name=Msa0550730
chr4_1	mRNA	46254774	46256528	46254774	ID=Msa0550730-mRNA-1;Parent=Msa0550730;Name=Msa0550730-mRNA-1;_AED=0.05;_eAED=0.05;_QI=380\|1\|1\|1\|1\|1\|3\|168\|259
chr4_1	exon	46254774	46255339	46254774	ID=Msa0550730-mRNA-1:exon:12325;Parent=Msa0550730-mRNA-1
chr4_1	exon	46255634	46256008	46255634	ID=Msa0550730-mRNA-1:exon:12326;Parent=Msa0550730-mRNA-1
chr4_1	exon	46256142	46256528	46256142	ID=Msa0550730-mRNA-1:exon:12327;Parent=Msa0550730-mRNA-1
chr4_1	five_prime_UTR	46254774	46255153	46254774	ID=Msa0550730-mRNA-1:five_prime_utr;Parent=Msa0550730-mRNA-1
chr4_1	CDS	46255154	46255339	46255154	ID=Msa0550730-mRNA-1:cds;Parent=Msa0550730-mRNA-1
chr4_1	CDS	46255634	46256008	46255634	ID=Msa0550730-mRNA-1:cds;Parent=Msa0550730-mRNA-1
chr4_1	CDS	46256142	46256360	46256142	ID=Msa0550730-mRNA-1:cds;Parent=Msa0550730-mRNA-1
chr4_1	three_prime_UTR	46256361	46256528	46256361	ID=Msa0550730-mRNA-1:three_prime_utr;Parent=Msa0550730-mRNA-1

Gene Sequence

>Msa0550730

ATGAAGATCCAGTGTGATGCATGTCACAAACAAGAGGCCTCCTTGTTCTGCCCTGCAGATGAAGCAGCTCTCTGCAATCAATGTGATCGCAATATCCACTATGCCAACAAGGTCTCTGCTAAACACAAACGCTTCACTCTTCACCACCCCACTTCCAAAGACACCCCTCTCTGTGATATCTGCAAGGAGAGGCGTGCATATCTATTTTGCAAAGAAGATAGAGCAATACTTTGCAGGGAATGTGACATTCCTATCCATGAAGTCAACAAACTTACCAAGCAACACAACAGGTTTCTTCTCACCGGCGTAAAGATCGGTGCTTGTTCTTCTTATTCAAACCCAACAACTTCCAATGGCTCAGAACTAAGAACCTCAAGTCCAAGACCAAGTTCATTTTCAAGTGAAAATAATAGTTGTTCTCAAAGTTCTTTCAAAGAAAACATGGCTTGTGACACAGTTTCAACCAGTAGCATTTCCGAATACTTGATTGAAACAATTCCTGGTTACTGCATGGAAGACCTTTTTGATGCTTCTTTTACACCTAATAACGTTTTCTGTAAGGATTATCTTGAACAGAACCAAGATCTTCAAGTTATAAACATGTCTGACTGGGTACCACAATCTCAAGTTAGATTCCCTCAGCTTAGCGCTAATTCGAATGTTCCCCACATTGATTCTTTAGACGGGGTTATGAAAATGCAACATACTAAAGCTGTTGAAGGATACAAAGTTCCTTCAATTAGTCCTCCATTCTTCAAACAATGTACAGGCACTCGTTAA

Protein sequence

>Msa0550730

MKIQCDACHKQEASLFCPADEAALCNQCDRNIHYANKVSAKHKRFTLHHPTSKDTPLCDICKERRAYLFCKEDRAILCRECDIPIHEVNKLTKQHNRFLLTGVKIGACSSYSNPTTSNGSELRTSSPRPSSFSSENNSCSQSSFKENMACDTVSTSSISEYLIETIPGYCMEDLFDASFTPNNVFCKDYLEQNQDLQVINMSDWVPQSQVRFPQLSANSNVPHIDSLDGVMKMQHTKAVEGYKVPSISPPFFKQCTGTR*