AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0550730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550730 MtrunA17_Chr4g0035651 95.982 224 8 1 1 223 1 224 9.24e-160 442
Msa0550730 MtrunA17_Chr3g0145061 59.624 213 69 6 1 198 1 211 3.66e-76 230
Msa0550730 MtrunA17_Chr1g0155811 47.926 217 99 4 1 208 1 212 6.00e-58 186
Msa0550730 MtrunA17_Chr3g0142221 47.191 178 58 3 1 177 1 143 3.55e-51 166
Msa0550730 MtrunA17_Chr2g0314671 54.717 106 47 1 1 106 1 105 9.81e-36 129
Msa0550730 MtrunA17_Chr4g0033641 50.000 110 54 1 1 110 1 109 5.29e-34 123
Msa0550730 MtrunA17_Chr2g0329511 49.057 106 53 1 1 106 1 105 2.67e-31 115
Msa0550730 MtrunA17_Chr5g0405151 44.000 100 52 2 1 100 1 96 1.45e-22 91.7
Msa0550730 MtrunA17_Chr1g0150471 43.299 97 45 1 5 101 6 92 1.11e-19 86.7
Msa0550730 MtrunA17_Chr3g0136701 39.175 97 49 1 5 101 6 92 1.25e-16 77.8
Msa0550730 MtrunA17_Chr4g0072871 40.860 93 45 1 5 97 22 104 2.37e-16 77.8
Msa0550730 MtrunA17_Chr7g0220721 35.043 117 63 2 5 121 26 129 5.15e-15 73.9
Msa0550730 MtrunA17_Chr5g0429341 36.082 97 52 1 1 97 1 87 6.92e-14 70.9
Msa0550730 MtrunA17_Chr3g0120711 35.052 97 52 2 1 97 1 86 2.39e-11 63.2
Msa0550730 MtrunA17_Chr7g0268691 32.653 98 55 2 5 102 20 106 9.36e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0550730 AT4G39070.1 52.500 200 71 4 1 176 1 200 6.21e-58 185
Msa0550730 AT1G75540.1 35.938 320 134 9 1 250 1 319 6.20e-55 180
Msa0550730 AT1G06040.2 46.715 137 66 2 1 137 1 130 4.67e-36 126
Msa0550730 AT1G78600.1 42.529 174 91 3 1 173 1 166 1.05e-35 129
Msa0550730 AT1G78600.2 42.529 174 91 3 1 173 1 166 1.21e-35 129
Msa0550730 AT1G06040.1 46.715 137 66 2 1 137 1 130 3.59e-35 126
Msa0550730 AT2G31380.1 50.442 113 55 1 1 113 1 112 1.53e-32 119
Msa0550730 AT4G10240.1 45.000 140 56 4 1 135 1 124 4.76e-30 110
Msa0550730 AT4G38960.1 43.810 105 55 2 1 105 1 101 3.36e-23 93.6
Msa0550730 AT4G38960.6 43.810 105 55 2 1 105 1 101 3.36e-23 93.6
Msa0550730 AT2G21320.1 43.810 105 55 2 1 105 1 101 6.25e-22 89.7
Msa0550730 AT2G24790.2 41.237 97 47 1 5 101 8 94 6.83e-19 82.8
Msa0550730 AT2G24790.1 41.237 97 47 1 5 101 8 94 1.80e-18 83.2
Msa0550730 AT5G24930.1 37.113 97 51 1 5 101 6 92 3.90e-16 77.4
Msa0550730 AT3G02380.1 34.906 106 59 1 5 110 16 111 8.06e-16 76.3
Msa0550730 AT4G38960.3 31.081 148 55 3 1 105 1 144 3.07e-15 73.2
Msa0550730 AT5G15850.1 32.520 123 70 2 5 127 12 121 3.75e-15 74.7
Msa0550730 AT5G15840.2 32.520 123 71 2 5 127 20 130 2.18e-14 71.6
Msa0550730 AT5G15840.1 32.520 123 71 2 5 127 20 130 2.87e-14 72.0
Msa0550730 AT2G47890.2 40.244 82 39 1 5 86 13 84 2.61e-12 65.1
Msa0550730 AT1G28050.1 35.644 101 55 1 2 102 6 96 3.43e-12 66.2
Msa0550730 AT2G47890.3 40.244 82 39 1 5 86 13 84 4.05e-12 65.5
Msa0550730 AT5G48250.1 34.694 98 53 2 1 97 1 88 6.21e-12 65.1
Msa0550730 AT2G47890.1 40.244 82 39 1 5 86 13 84 7.04e-12 65.1
Msa0550730 AT2G33500.2 34.000 100 56 1 3 102 10 99 1.32e-11 64.3
Msa0550730 AT2G33500.1 34.000 100 56 1 3 102 10 99 1.40e-11 64.3
Msa0550730 AT5G57660.1 37.634 93 44 2 5 97 22 100 2.24e-11 63.5
Msa0550730 AT3G07650.3 36.047 86 45 1 1 86 1 76 6.67e-11 62.4
Msa0550730 AT3G07650.2 36.047 86 45 1 1 86 1 76 6.67e-11 62.4
Msa0550730 AT3G07650.4 36.047 86 45 1 1 86 1 76 6.67e-11 62.4
Msa0550730 AT3G07650.1 36.047 86 45 1 1 86 1 76 6.67e-11 62.4

Find 85 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGGATCTGATGAATTA+TGG 0.164578 4_1:-46254948 None:intergenic
GGATGGGGACAAAAGAATTA+TGG 0.300907 4_1:-46255077 None:intergenic
AATTGTTTCAATCAAGTATT+CGG 0.312961 4_1:-46255923 None:intergenic
ACCTTACAGAAAACGTTATT+AGG 0.333966 4_1:-46255988 None:intergenic
ATCACAGAGAGGGGTGTCTT+TGG 0.337373 4_1:-46255308 None:intergenic
CACCTTTACTACATTAACTT+TGG 0.337392 4_1:+46255044 Msa0550730:five_prime_UTR
TGTGCTATTTCTACTACATT+TGG 0.337992 4_1:-46254964 None:intergenic
ATTTGGATCTGATGAATTAT+GGG 0.352913 4_1:-46254947 None:intergenic
CTTGAAAATGAACTTGGTCT+TGG 0.353565 4_1:-46255826 None:intergenic
TGTTTATAACTTGAAGATCT+TGG 0.361700 4_1:-46256159 None:intergenic
CTGAGCCATTGGAAGTTGTT+GGG 0.365142 4_1:-46255786 None:intergenic
TGGTAAGTTTGTTGACTTCA+TGG 0.370579 4_1:-46255702 None:intergenic
TTGTTCTCATCTAAGGTAGA+AGG 0.378807 4_1:+46256450 Msa0550730:three_prime_UTR
AAGATAGAGCAATACTTTGC+AGG 0.379068 4_1:+46255662 Msa0550730:CDS
TCTGAGCCATTGGAAGTTGT+TGG 0.394596 4_1:-46255787 None:intergenic
TACTTGATTGAAACAATTCC+TGG 0.400109 4_1:+46255928 Msa0550730:CDS
CAGAGAGCTGCTTCATCTGC+AGG 0.413650 4_1:-46255205 None:intergenic
GAGAGAGTCAGAAAATAAAT+AGG 0.421018 4_1:+46254807 Msa0550730:five_prime_UTR
CAGAGAGAACAGCTAAGATT+TGG 0.425768 4_1:-46254895 None:intergenic
CAACATACTAAAGCTGTTGA+AGG 0.434663 4_1:+46256280 Msa0550730:CDS
CCCCACATTGATTCTTTAGA+CGG 0.435559 4_1:+46256244 Msa0550730:CDS
ATGGATAAGAAAACAAATGA+TGG 0.444047 4_1:-46256395 None:intergenic
AGATAATTCGTATATGAAAC+GGG 0.445044 4_1:-46256510 None:intergenic
GTGTAAAAGAAGCATCAAAA+AGG 0.446259 4_1:-46255966 None:intergenic
AAGTTATAAACATGTCTGAC+TGG 0.451524 4_1:+46256170 Msa0550730:CDS
TAGATAATTCGTATATGAAA+CGG 0.454093 4_1:-46256511 None:intergenic
AACTTACCAAGCAACACAAC+AGG 0.456566 4_1:+46255716 Msa0550730:CDS
TTGAAGGCAAAGTGGGGATG+GGG 0.458406 4_1:-46255092 None:intergenic
TGAACTTGGTCTTGGACTTG+AGG 0.462812 4_1:-46255818 None:intergenic
GTGTTTAGCAGAGACCTTGT+TGG 0.467471 4_1:-46255257 None:intergenic
CTTCTCACCGGCGTAAAGAT+CGG 0.467524 4_1:+46255742 Msa0550730:CDS
CAGAGACCTTGTTGGCATAG+TGG 0.468511 4_1:-46255249 None:intergenic
CCCACATTGATTCTTTAGAC+GGG 0.468902 4_1:+46256245 Msa0550730:CDS
TTTGAAGGCAAAGTGGGGAT+GGG 0.471662 4_1:-46255093 None:intergenic
GATGGGGACAAAAGAATTAT+GGG 0.472346 4_1:-46255076 None:intergenic
GAGAGAAGTACATAATTAAG+AGG 0.479528 4_1:-46256477 None:intergenic
CAAGTATTCGGAAATGCTAC+TGG 0.481122 4_1:-46255911 None:intergenic
CCTGTACATTGTTTGAAGAA+TGG 0.484919 4_1:-46256328 None:intergenic
TTCGTATATGAAACGGGAAA+AGG 0.485996 4_1:-46256504 None:intergenic
TCTGATGAATTATGGGCGTT+TGG 0.486017 4_1:-46254940 None:intergenic
TTCCAAAGTTAATGTAGTAA+AGG 0.489803 4_1:-46255046 None:intergenic
GGTTCTTAGTTCTGAGCCAT+TGG 0.490954 4_1:-46255797 None:intergenic
AAGAATGGAGGACTAATTGA+AGG 0.493324 4_1:-46256313 None:intergenic
AGAGGGGTGTCTTTGGAAGT+GGG 0.493908 4_1:-46255301 None:intergenic
GCCATTGATTTAAAATCGAT+CGG 0.501102 4_1:+46255012 Msa0550730:five_prime_UTR
GAGAGGGGTGTCTTTGGAAG+TGG 0.504462 4_1:-46255302 None:intergenic
TTACACGCATTAGTTGATCT+AGG 0.518328 4_1:+46254990 Msa0550730:five_prime_UTR
TTCTCTTTCTATGAGTAAAG+AGG 0.529085 4_1:-46254775 None:intergenic
AAGAAACCTGTTGTGTTGCT+TGG 0.533532 4_1:-46255722 None:intergenic
AGATAGAGCAATACTTTGCA+GGG 0.534009 4_1:+46255663 Msa0550730:CDS
AACAATTCCTGGTTACTGCA+TGG 0.545989 4_1:+46255939 Msa0550730:CDS
AAAATAAATAGGTCTCGTAA+TGG 0.547529 4_1:+46254818 Msa0550730:five_prime_UTR
TTCATCTGCAGGGCAGAACA+AGG 0.547884 4_1:-46255194 None:intergenic
TGGATAAGAAAACAAATGAT+GGG 0.550125 4_1:-46256394 None:intergenic
CACAACAGGTTTCTTCTCAC+CGG 0.551440 4_1:+46255730 Msa0550730:CDS
TCGAATTAGCGCTAAGCTGA+GGG 0.554345 4_1:-46256216 None:intergenic
CCATTCTTCAAACAATGTAC+AGG 0.558397 4_1:+46256328 Msa0550730:CDS
AAGTTCTTTCAAAGAAAACA+TGG 0.562402 4_1:+46255870 Msa0550730:CDS
TCAAACCCAACAACTTCCAA+TGG 0.565279 4_1:+46255781 Msa0550730:CDS
AGGTCTTCCATGCAGTAACC+AGG 0.571610 4_1:-46255946 None:intergenic
AGTTTGTTGACTTCATGGAT+AGG 0.572223 4_1:-46255697 None:intergenic
GAGGGGTGTCTTTGGAAGTG+GGG 0.580604 4_1:-46255300 None:intergenic
GGGTGTCTTTGGAAGTGGGG+TGG 0.581849 4_1:-46255297 None:intergenic
TCTCTTTCTATGAGTAAAGA+GGG 0.582196 4_1:-46254774 None:intergenic
TTCGAATTAGCGCTAAGCTG+AGG 0.584714 4_1:-46256217 None:intergenic
ATATGAAACGGGAAAAGGAA+AGG 0.589122 4_1:-46256499 None:intergenic
AGAGAGCTGCTTCATCTGCA+GGG 0.589609 4_1:-46255204 None:intergenic
GTACATTGTTTGAAGAATGG+AGG 0.591485 4_1:-46256325 None:intergenic
GGGAATCTAACTTGAGATTG+TGG 0.592001 4_1:-46256196 None:intergenic
CCCGTCTAAAGAATCAATGT+GGG 0.595981 4_1:-46256245 None:intergenic
CAATATCCACTATGCCAACA+AGG 0.603596 4_1:+46255243 Msa0550730:CDS
CCACATTGATTCTTTAGACG+GGG 0.606501 4_1:+46256246 Msa0550730:CDS
CCTTGCAGATATCACAGAGA+GGG 0.606602 4_1:-46255318 None:intergenic
CCCTCTCTGTGATATCTGCA+AGG 0.608181 4_1:+46255318 Msa0550730:CDS
CCCCGTCTAAAGAATCAATG+TGG 0.617525 4_1:-46256246 None:intergenic
GTTTGTGACATGCATCACAC+TGG 0.618408 4_1:-46255162 None:intergenic
TGATGCATGTCACAAACAAG+AGG 0.622158 4_1:+46255168 Msa0550730:CDS
AGTTATAAACATGTCTGACT+GGG 0.637692 4_1:+46256171 Msa0550730:CDS
TCTCATCTAAGGTAGAAGGT+AGG 0.670836 4_1:+46256454 Msa0550730:three_prime_UTR
CATAGAAAGAGAATCACACA+TGG 0.682829 4_1:+46254785 Msa0550730:five_prime_UTR
ATCTGCAGGGCAGAACAAGG+AGG 0.684576 4_1:-46255191 None:intergenic
CTTGCAGATATCACAGAGAG+GGG 0.687405 4_1:-46255317 None:intergenic
CCGTCTAAAGAATCAATGTG+GGG 0.706719 4_1:-46256244 None:intergenic
ACAAGCACCGATCTTTACGC+CGG 0.707715 4_1:-46255749 None:intergenic
ACCTTGCAGATATCACAGAG+AGG 0.718667 4_1:-46255319 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAGTTGTATATGTTTTT+TGG + chr4_1:46256108-46256127 Msa0550730:intron 15.0%
!! TTCTAGATAAAAATTGTAAA+TGG - chr4_1:46256417-46256436 None:intergenic 15.0%
!!! AATAAGTTGTATATGTTTTT+TGG + chr4_1:46256108-46256127 Msa0550730:intron 15.0%
!! TTCTAGATAAAAATTGTAAA+TGG - chr4_1:46256417-46256436 None:intergenic 15.0%
!!! TTCTGAAAATCAGTTAAATA+AGG - chr4_1:46255464-46255483 None:intergenic 20.0%
!! AAGTTGTTTATCGATAAAAT+AGG + chr4_1:46255512-46255531 Msa0550730:intron 20.0%
!!! AACAAATATGACCATATTTT+TGG - chr4_1:46255561-46255580 None:intergenic 20.0%
!! AATAAAAGACTGAGTAATAT+AGG + chr4_1:46255591-46255610 Msa0550730:intron 20.0%
!! AATTGTTTCAATCAAGTATT+CGG - chr4_1:46255926-46255945 None:intergenic 20.0%
!!! TTCTGAAAATCAGTTAAATA+AGG - chr4_1:46255464-46255483 None:intergenic 20.0%
!! AAGTTGTTTATCGATAAAAT+AGG + chr4_1:46255512-46255531 Msa0550730:intron 20.0%
!!! AACAAATATGACCATATTTT+TGG - chr4_1:46255561-46255580 None:intergenic 20.0%
!! AATAAAAGACTGAGTAATAT+AGG + chr4_1:46255591-46255610 Msa0550730:intron 20.0%
!! AATTGTTTCAATCAAGTATT+CGG - chr4_1:46255926-46255945 None:intergenic 20.0%
! AAAATAAATAGGTCTCGTAA+TGG + chr4_1:46254818-46254837 Msa0550730:five_prime_UTR 25.0%
! ATCTGACTTTCAGAAAAATT+TGG - chr4_1:46254851-46254870 None:intergenic 25.0%
! ATTTGGATCTGATGAATTAT+GGG - chr4_1:46254950-46254969 None:intergenic 25.0%
! TTCCAAAGTTAATGTAGTAA+AGG - chr4_1:46255049-46255068 None:intergenic 25.0%
!!! ACCATATTTTTGGATCATAT+AGG - chr4_1:46255551-46255570 None:intergenic 25.0%
!! GGAAATAACATTTTTATTGC+AGG + chr4_1:46255612-46255631 Msa0550730:intron 25.0%
!! TTTTCACTTGAAAATGAACT+TGG - chr4_1:46255835-46255854 None:intergenic 25.0%
! AAGTTCTTTCAAAGAAAACA+TGG + chr4_1:46255870-46255889 Msa0550730:CDS 25.0%
!!! ATGTTTTTTGGTATTGTTTC+AGG + chr4_1:46256120-46256139 Msa0550730:intron 25.0%
! TGTTTATAACTTGAAGATCT+TGG - chr4_1:46256162-46256181 None:intergenic 25.0%
! TGGATAAGAAAACAAATGAT+GGG - chr4_1:46256397-46256416 None:intergenic 25.0%
! ATGGATAAGAAAACAAATGA+TGG - chr4_1:46256398-46256417 None:intergenic 25.0%
! AAAATAAATAGGTCTCGTAA+TGG + chr4_1:46254818-46254837 Msa0550730:five_prime_UTR 25.0%
! ATCTGACTTTCAGAAAAATT+TGG - chr4_1:46254851-46254870 None:intergenic 25.0%
! ATTTGGATCTGATGAATTAT+GGG - chr4_1:46254950-46254969 None:intergenic 25.0%
! TTCCAAAGTTAATGTAGTAA+AGG - chr4_1:46255049-46255068 None:intergenic 25.0%
!!! ACCATATTTTTGGATCATAT+AGG - chr4_1:46255551-46255570 None:intergenic 25.0%
!! GGAAATAACATTTTTATTGC+AGG + chr4_1:46255612-46255631 Msa0550730:intron 25.0%
!! TTTTCACTTGAAAATGAACT+TGG - chr4_1:46255835-46255854 None:intergenic 25.0%
! AAGTTCTTTCAAAGAAAACA+TGG + chr4_1:46255870-46255889 Msa0550730:CDS 25.0%
!!! ATGTTTTTTGGTATTGTTTC+AGG + chr4_1:46256120-46256139 Msa0550730:intron 25.0%
! TGTTTATAACTTGAAGATCT+TGG - chr4_1:46256162-46256181 None:intergenic 25.0%
! TGGATAAGAAAACAAATGAT+GGG - chr4_1:46256397-46256416 None:intergenic 25.0%
! ATGGATAAGAAAACAAATGA+TGG - chr4_1:46256398-46256417 None:intergenic 25.0%
TCTCTTTCTATGAGTAAAGA+GGG - chr4_1:46254777-46254796 None:intergenic 30.0%
TTCTCTTTCTATGAGTAAAG+AGG - chr4_1:46254778-46254797 None:intergenic 30.0%
GAGAGAGTCAGAAAATAAAT+AGG + chr4_1:46254807-46254826 Msa0550730:five_prime_UTR 30.0%
CATTTGGATCTGATGAATTA+TGG - chr4_1:46254951-46254970 None:intergenic 30.0%
!! TGTGCTATTTCTACTACATT+TGG - chr4_1:46254967-46254986 None:intergenic 30.0%
!! GCCATTGATTTAAAATCGAT+CGG + chr4_1:46255012-46255031 Msa0550730:five_prime_UTR 30.0%
CACCTTTACTACATTAACTT+TGG + chr4_1:46255044-46255063 Msa0550730:five_prime_UTR 30.0%
!!! TTTTGTTTTGAAGGCAAAGT+GGG - chr4_1:46255102-46255121 None:intergenic 30.0%
!!! TTTTTGTTTTGAAGGCAAAG+TGG - chr4_1:46255103-46255122 None:intergenic 30.0%
!!! AGGTTTGGTTTTTGTTTTGA+AGG - chr4_1:46255111-46255130 None:intergenic 30.0%
! TTTCTTACGTACTTTTCAAC+AGG + chr4_1:46255356-46255375 Msa0550730:intron 30.0%
! TTCTTACGTACTTTTCAACA+GGG + chr4_1:46255357-46255376 Msa0550730:intron 30.0%
GCCTATATGATCCAAAAATA+TGG + chr4_1:46255547-46255566 Msa0550730:intron 30.0%
! TAACATTTTTATTGCAGGAG+AGG + chr4_1:46255617-46255636 Msa0550730:intron 30.0%
TACTTGATTGAAACAATTCC+TGG + chr4_1:46255928-46255947 Msa0550730:CDS 30.0%
!! GTGTAAAAGAAGCATCAAAA+AGG - chr4_1:46255969-46255988 None:intergenic 30.0%
! ACCTAATAACGTTTTCTGTA+AGG + chr4_1:46255987-46256006 Msa0550730:CDS 30.0%
ACCTTACAGAAAACGTTATT+AGG - chr4_1:46255991-46256010 None:intergenic 30.0%
!! AACGTTTTCTGTAAGGTATT+TGG + chr4_1:46255994-46256013 Msa0550730:intron 30.0%
TAGATGAAAAATAGCTAGCT+AGG - chr4_1:46256075-46256094 None:intergenic 30.0%
AAGTTATAAACATGTCTGAC+TGG + chr4_1:46256170-46256189 Msa0550730:CDS 30.0%
AGTTATAAACATGTCTGACT+GGG + chr4_1:46256171-46256190 Msa0550730:CDS 30.0%
! TGCAATTTTGTTCTCATCTA+AGG + chr4_1:46256443-46256462 Msa0550730:three_prime_UTR 30.0%
! GAGAGAAGTACATAATTAAG+AGG - chr4_1:46256480-46256499 None:intergenic 30.0%
TCTCTTTCTATGAGTAAAGA+GGG - chr4_1:46254777-46254796 None:intergenic 30.0%
TTCTCTTTCTATGAGTAAAG+AGG - chr4_1:46254778-46254797 None:intergenic 30.0%
GAGAGAGTCAGAAAATAAAT+AGG + chr4_1:46254807-46254826 Msa0550730:five_prime_UTR 30.0%
CATTTGGATCTGATGAATTA+TGG - chr4_1:46254951-46254970 None:intergenic 30.0%
!! TGTGCTATTTCTACTACATT+TGG - chr4_1:46254967-46254986 None:intergenic 30.0%
!! GCCATTGATTTAAAATCGAT+CGG + chr4_1:46255012-46255031 Msa0550730:five_prime_UTR 30.0%
CACCTTTACTACATTAACTT+TGG + chr4_1:46255044-46255063 Msa0550730:five_prime_UTR 30.0%
!!! TTTTGTTTTGAAGGCAAAGT+GGG - chr4_1:46255102-46255121 None:intergenic 30.0%
!!! TTTTTGTTTTGAAGGCAAAG+TGG - chr4_1:46255103-46255122 None:intergenic 30.0%
!!! AGGTTTGGTTTTTGTTTTGA+AGG - chr4_1:46255111-46255130 None:intergenic 30.0%
! TTTCTTACGTACTTTTCAAC+AGG + chr4_1:46255356-46255375 Msa0550730:intron 30.0%
! TTCTTACGTACTTTTCAACA+GGG + chr4_1:46255357-46255376 Msa0550730:intron 30.0%
GCCTATATGATCCAAAAATA+TGG + chr4_1:46255547-46255566 Msa0550730:intron 30.0%
! TAACATTTTTATTGCAGGAG+AGG + chr4_1:46255617-46255636 Msa0550730:intron 30.0%
TACTTGATTGAAACAATTCC+TGG + chr4_1:46255928-46255947 Msa0550730:CDS 30.0%
!! GTGTAAAAGAAGCATCAAAA+AGG - chr4_1:46255969-46255988 None:intergenic 30.0%
! ACCTAATAACGTTTTCTGTA+AGG + chr4_1:46255987-46256006 Msa0550730:CDS 30.0%
ACCTTACAGAAAACGTTATT+AGG - chr4_1:46255991-46256010 None:intergenic 30.0%
!! AACGTTTTCTGTAAGGTATT+TGG + chr4_1:46255994-46256013 Msa0550730:intron 30.0%
TAGATGAAAAATAGCTAGCT+AGG - chr4_1:46256075-46256094 None:intergenic 30.0%
AAGTTATAAACATGTCTGAC+TGG + chr4_1:46256170-46256189 Msa0550730:CDS 30.0%
AGTTATAAACATGTCTGACT+GGG + chr4_1:46256171-46256190 Msa0550730:CDS 30.0%
! TGCAATTTTGTTCTCATCTA+AGG + chr4_1:46256443-46256462 Msa0550730:three_prime_UTR 30.0%
! GAGAGAAGTACATAATTAAG+AGG - chr4_1:46256480-46256499 None:intergenic 30.0%
CATAGAAAGAGAATCACACA+TGG + chr4_1:46254785-46254804 Msa0550730:five_prime_UTR 35.0%
TTACACGCATTAGTTGATCT+AGG + chr4_1:46254990-46255009 Msa0550730:five_prime_UTR 35.0%
!! GCCGATCGATTTTAAATCAA+TGG - chr4_1:46255016-46255035 None:intergenic 35.0%
GATGGGGACAAAAGAATTAT+GGG - chr4_1:46255079-46255098 None:intergenic 35.0%
!!! TTTGTTTTGAAGGCAAAGTG+GGG - chr4_1:46255101-46255120 None:intergenic 35.0%
!!! TCTATTTTTGAGCGTTTTGG+AGG - chr4_1:46255131-46255150 None:intergenic 35.0%
!!! CTCTCTATTTTTGAGCGTTT+TGG - chr4_1:46255134-46255153 None:intergenic 35.0%
AAGATAGAGCAATACTTTGC+AGG + chr4_1:46255662-46255681 Msa0550730:CDS 35.0%
AGATAGAGCAATACTTTGCA+GGG + chr4_1:46255663-46255682 Msa0550730:CDS 35.0%
!! AGTTTGTTGACTTCATGGAT+AGG - chr4_1:46255700-46255719 None:intergenic 35.0%
!! TGGTAAGTTTGTTGACTTCA+TGG - chr4_1:46255705-46255724 None:intergenic 35.0%
CTTGAAAATGAACTTGGTCT+TGG - chr4_1:46255829-46255848 None:intergenic 35.0%
CAACATACTAAAGCTGTTGA+AGG + chr4_1:46256280-46256299 Msa0550730:CDS 35.0%
AAGAATGGAGGACTAATTGA+AGG - chr4_1:46256316-46256335 None:intergenic 35.0%
GTACATTGTTTGAAGAATGG+AGG - chr4_1:46256328-46256347 None:intergenic 35.0%
CCTGTACATTGTTTGAAGAA+TGG - chr4_1:46256331-46256350 None:intergenic 35.0%
CCATTCTTCAAACAATGTAC+AGG + chr4_1:46256328-46256347 Msa0550730:CDS 35.0%
! TTGTTCTCATCTAAGGTAGA+AGG + chr4_1:46256450-46256469 Msa0550730:three_prime_UTR 35.0%
ATATGAAACGGGAAAAGGAA+AGG - chr4_1:46256502-46256521 None:intergenic 35.0%
TTCGTATATGAAACGGGAAA+AGG - chr4_1:46256507-46256526 None:intergenic 35.0%
CATAGAAAGAGAATCACACA+TGG + chr4_1:46254785-46254804 Msa0550730:five_prime_UTR 35.0%
TTACACGCATTAGTTGATCT+AGG + chr4_1:46254990-46255009 Msa0550730:five_prime_UTR 35.0%
!! GCCGATCGATTTTAAATCAA+TGG - chr4_1:46255016-46255035 None:intergenic 35.0%
GATGGGGACAAAAGAATTAT+GGG - chr4_1:46255079-46255098 None:intergenic 35.0%
!!! TTTGTTTTGAAGGCAAAGTG+GGG - chr4_1:46255101-46255120 None:intergenic 35.0%
!!! TCTATTTTTGAGCGTTTTGG+AGG - chr4_1:46255131-46255150 None:intergenic 35.0%
!!! CTCTCTATTTTTGAGCGTTT+TGG - chr4_1:46255134-46255153 None:intergenic 35.0%
AAGATAGAGCAATACTTTGC+AGG + chr4_1:46255662-46255681 Msa0550730:CDS 35.0%
AGATAGAGCAATACTTTGCA+GGG + chr4_1:46255663-46255682 Msa0550730:CDS 35.0%
!! AGTTTGTTGACTTCATGGAT+AGG - chr4_1:46255700-46255719 None:intergenic 35.0%
!! TGGTAAGTTTGTTGACTTCA+TGG - chr4_1:46255705-46255724 None:intergenic 35.0%
CTTGAAAATGAACTTGGTCT+TGG - chr4_1:46255829-46255848 None:intergenic 35.0%
CAACATACTAAAGCTGTTGA+AGG + chr4_1:46256280-46256299 Msa0550730:CDS 35.0%
AAGAATGGAGGACTAATTGA+AGG - chr4_1:46256316-46256335 None:intergenic 35.0%
GTACATTGTTTGAAGAATGG+AGG - chr4_1:46256328-46256347 None:intergenic 35.0%
CCTGTACATTGTTTGAAGAA+TGG - chr4_1:46256331-46256350 None:intergenic 35.0%
CCATTCTTCAAACAATGTAC+AGG + chr4_1:46256328-46256347 Msa0550730:CDS 35.0%
! TTGTTCTCATCTAAGGTAGA+AGG + chr4_1:46256450-46256469 Msa0550730:three_prime_UTR 35.0%
ATATGAAACGGGAAAAGGAA+AGG - chr4_1:46256502-46256521 None:intergenic 35.0%
TTCGTATATGAAACGGGAAA+AGG - chr4_1:46256507-46256526 None:intergenic 35.0%
CAGAGAGAACAGCTAAGATT+TGG - chr4_1:46254898-46254917 None:intergenic 40.0%
TCTGATGAATTATGGGCGTT+TGG - chr4_1:46254943-46254962 None:intergenic 40.0%
GGATGGGGACAAAAGAATTA+TGG - chr4_1:46255080-46255099 None:intergenic 40.0%
!!! TTTTGAGCGTTTTGGAGGTT+TGG - chr4_1:46255126-46255145 None:intergenic 40.0%
TGATGCATGTCACAAACAAG+AGG + chr4_1:46255168-46255187 Msa0550730:CDS 40.0%
CAATATCCACTATGCCAACA+AGG + chr4_1:46255243-46255262 Msa0550730:CDS 40.0%
AACTTACCAAGCAACACAAC+AGG + chr4_1:46255716-46255735 Msa0550730:CDS 40.0%
! AAGAAACCTGTTGTGTTGCT+TGG - chr4_1:46255725-46255744 None:intergenic 40.0%
TCAAACCCAACAACTTCCAA+TGG + chr4_1:46255781-46255800 Msa0550730:CDS 40.0%
CAAGTATTCGGAAATGCTAC+TGG - chr4_1:46255914-46255933 None:intergenic 40.0%
AACAATTCCTGGTTACTGCA+TGG + chr4_1:46255939-46255958 Msa0550730:CDS 40.0%
GGGAATCTAACTTGAGATTG+TGG - chr4_1:46256199-46256218 None:intergenic 40.0%
CCGTCTAAAGAATCAATGTG+GGG - chr4_1:46256247-46256266 None:intergenic 40.0%
!! CCCCACATTGATTCTTTAGA+CGG + chr4_1:46256244-46256263 Msa0550730:CDS 40.0%
CCCGTCTAAAGAATCAATGT+GGG - chr4_1:46256248-46256267 None:intergenic 40.0%
!! CCCACATTGATTCTTTAGAC+GGG + chr4_1:46256245-46256264 Msa0550730:CDS 40.0%
!! CCACATTGATTCTTTAGACG+GGG + chr4_1:46256246-46256265 Msa0550730:CDS 40.0%
! TCTCATCTAAGGTAGAAGGT+AGG + chr4_1:46256454-46256473 Msa0550730:three_prime_UTR 40.0%
CAGAGAGAACAGCTAAGATT+TGG - chr4_1:46254898-46254917 None:intergenic 40.0%
TCTGATGAATTATGGGCGTT+TGG - chr4_1:46254943-46254962 None:intergenic 40.0%
GGATGGGGACAAAAGAATTA+TGG - chr4_1:46255080-46255099 None:intergenic 40.0%
!!! TTTTGAGCGTTTTGGAGGTT+TGG - chr4_1:46255126-46255145 None:intergenic 40.0%
TGATGCATGTCACAAACAAG+AGG + chr4_1:46255168-46255187 Msa0550730:CDS 40.0%
CAATATCCACTATGCCAACA+AGG + chr4_1:46255243-46255262 Msa0550730:CDS 40.0%
AACTTACCAAGCAACACAAC+AGG + chr4_1:46255716-46255735 Msa0550730:CDS 40.0%
! AAGAAACCTGTTGTGTTGCT+TGG - chr4_1:46255725-46255744 None:intergenic 40.0%
TCAAACCCAACAACTTCCAA+TGG + chr4_1:46255781-46255800 Msa0550730:CDS 40.0%
CAAGTATTCGGAAATGCTAC+TGG - chr4_1:46255914-46255933 None:intergenic 40.0%
AACAATTCCTGGTTACTGCA+TGG + chr4_1:46255939-46255958 Msa0550730:CDS 40.0%
GGGAATCTAACTTGAGATTG+TGG - chr4_1:46256199-46256218 None:intergenic 40.0%
CCGTCTAAAGAATCAATGTG+GGG - chr4_1:46256247-46256266 None:intergenic 40.0%
!! CCCCACATTGATTCTTTAGA+CGG + chr4_1:46256244-46256263 Msa0550730:CDS 40.0%
CCCGTCTAAAGAATCAATGT+GGG - chr4_1:46256248-46256267 None:intergenic 40.0%
!! CCCACATTGATTCTTTAGAC+GGG + chr4_1:46256245-46256264 Msa0550730:CDS 40.0%
!! CCACATTGATTCTTTAGACG+GGG + chr4_1:46256246-46256265 Msa0550730:CDS 40.0%
! TCTCATCTAAGGTAGAAGGT+AGG + chr4_1:46256454-46256473 Msa0550730:three_prime_UTR 40.0%
TTTGAAGGCAAAGTGGGGAT+GGG - chr4_1:46255096-46255115 None:intergenic 45.0%
!! TTTTGAAGGCAAAGTGGGGA+TGG - chr4_1:46255097-46255116 None:intergenic 45.0%
GTTTGTGACATGCATCACAC+TGG - chr4_1:46255165-46255184 None:intergenic 45.0%
GTGTTTAGCAGAGACCTTGT+TGG - chr4_1:46255260-46255279 None:intergenic 45.0%
CTTGCAGATATCACAGAGAG+GGG - chr4_1:46255320-46255339 None:intergenic 45.0%
CCTTGCAGATATCACAGAGA+GGG - chr4_1:46255321-46255340 None:intergenic 45.0%
ACCTTGCAGATATCACAGAG+AGG - chr4_1:46255322-46255341 None:intergenic 45.0%
CACAACAGGTTTCTTCTCAC+CGG + chr4_1:46255730-46255749 Msa0550730:CDS 45.0%
CTGAGCCATTGGAAGTTGTT+GGG - chr4_1:46255789-46255808 None:intergenic 45.0%
TCTGAGCCATTGGAAGTTGT+TGG - chr4_1:46255790-46255809 None:intergenic 45.0%
!! GGTTCTTAGTTCTGAGCCAT+TGG - chr4_1:46255800-46255819 None:intergenic 45.0%
!! TGAACTTGGTCTTGGACTTG+AGG - chr4_1:46255821-46255840 None:intergenic 45.0%
TCGAATTAGCGCTAAGCTGA+GGG - chr4_1:46256219-46256238 None:intergenic 45.0%
TTCGAATTAGCGCTAAGCTG+AGG - chr4_1:46256220-46256239 None:intergenic 45.0%
CCCCGTCTAAAGAATCAATG+TGG - chr4_1:46256249-46256268 None:intergenic 45.0%
TTTGAAGGCAAAGTGGGGAT+GGG - chr4_1:46255096-46255115 None:intergenic 45.0%
!! TTTTGAAGGCAAAGTGGGGA+TGG - chr4_1:46255097-46255116 None:intergenic 45.0%
GTTTGTGACATGCATCACAC+TGG - chr4_1:46255165-46255184 None:intergenic 45.0%
GTGTTTAGCAGAGACCTTGT+TGG - chr4_1:46255260-46255279 None:intergenic 45.0%
CTTGCAGATATCACAGAGAG+GGG - chr4_1:46255320-46255339 None:intergenic 45.0%
CCTTGCAGATATCACAGAGA+GGG - chr4_1:46255321-46255340 None:intergenic 45.0%
ACCTTGCAGATATCACAGAG+AGG - chr4_1:46255322-46255341 None:intergenic 45.0%
CACAACAGGTTTCTTCTCAC+CGG + chr4_1:46255730-46255749 Msa0550730:CDS 45.0%
CTGAGCCATTGGAAGTTGTT+GGG - chr4_1:46255789-46255808 None:intergenic 45.0%
TCTGAGCCATTGGAAGTTGT+TGG - chr4_1:46255790-46255809 None:intergenic 45.0%
!! GGTTCTTAGTTCTGAGCCAT+TGG - chr4_1:46255800-46255819 None:intergenic 45.0%
!! TGAACTTGGTCTTGGACTTG+AGG - chr4_1:46255821-46255840 None:intergenic 45.0%
TCGAATTAGCGCTAAGCTGA+GGG - chr4_1:46256219-46256238 None:intergenic 45.0%
TTCGAATTAGCGCTAAGCTG+AGG - chr4_1:46256220-46256239 None:intergenic 45.0%
CCCCGTCTAAAGAATCAATG+TGG - chr4_1:46256249-46256268 None:intergenic 45.0%
TTGAAGGCAAAGTGGGGATG+GGG - chr4_1:46255095-46255114 None:intergenic 50.0%
TTCATCTGCAGGGCAGAACA+AGG - chr4_1:46255197-46255216 None:intergenic 50.0%
AGAGAGCTGCTTCATCTGCA+GGG - chr4_1:46255207-46255226 None:intergenic 50.0%
CAGAGACCTTGTTGGCATAG+TGG - chr4_1:46255252-46255271 None:intergenic 50.0%
!! AGAGGGGTGTCTTTGGAAGT+GGG - chr4_1:46255304-46255323 None:intergenic 50.0%
!! ATCACAGAGAGGGGTGTCTT+TGG - chr4_1:46255311-46255330 None:intergenic 50.0%
CCCTCTCTGTGATATCTGCA+AGG + chr4_1:46255318-46255337 Msa0550730:CDS 50.0%
CTTCTCACCGGCGTAAAGAT+CGG + chr4_1:46255742-46255761 Msa0550730:CDS 50.0%
!! ACAAGCACCGATCTTTACGC+CGG - chr4_1:46255752-46255771 None:intergenic 50.0%
AGGTCTTCCATGCAGTAACC+AGG - chr4_1:46255949-46255968 None:intergenic 50.0%
TTGAAGGCAAAGTGGGGATG+GGG - chr4_1:46255095-46255114 None:intergenic 50.0%
TTCATCTGCAGGGCAGAACA+AGG - chr4_1:46255197-46255216 None:intergenic 50.0%
AGAGAGCTGCTTCATCTGCA+GGG - chr4_1:46255207-46255226 None:intergenic 50.0%
CAGAGACCTTGTTGGCATAG+TGG - chr4_1:46255252-46255271 None:intergenic 50.0%
!! AGAGGGGTGTCTTTGGAAGT+GGG - chr4_1:46255304-46255323 None:intergenic 50.0%
!! ATCACAGAGAGGGGTGTCTT+TGG - chr4_1:46255311-46255330 None:intergenic 50.0%
CCCTCTCTGTGATATCTGCA+AGG + chr4_1:46255318-46255337 Msa0550730:CDS 50.0%
CTTCTCACCGGCGTAAAGAT+CGG + chr4_1:46255742-46255761 Msa0550730:CDS 50.0%
!! ACAAGCACCGATCTTTACGC+CGG - chr4_1:46255752-46255771 None:intergenic 50.0%
AGGTCTTCCATGCAGTAACC+AGG - chr4_1:46255949-46255968 None:intergenic 50.0%
ATCTGCAGGGCAGAACAAGG+AGG - chr4_1:46255194-46255213 None:intergenic 55.0%
CAGAGAGCTGCTTCATCTGC+AGG - chr4_1:46255208-46255227 None:intergenic 55.0%
!! GAGGGGTGTCTTTGGAAGTG+GGG - chr4_1:46255303-46255322 None:intergenic 55.0%
!! GAGAGGGGTGTCTTTGGAAG+TGG - chr4_1:46255305-46255324 None:intergenic 55.0%
CTGCAACACAACCGCAGTAG+AGG + chr4_1:46255429-46255448 Msa0550730:intron 55.0%
GGCTGATACTGCCTCTACTG+CGG - chr4_1:46255443-46255462 None:intergenic 55.0%
ATCTGCAGGGCAGAACAAGG+AGG - chr4_1:46255194-46255213 None:intergenic 55.0%
CAGAGAGCTGCTTCATCTGC+AGG - chr4_1:46255208-46255227 None:intergenic 55.0%
!! GAGGGGTGTCTTTGGAAGTG+GGG - chr4_1:46255303-46255322 None:intergenic 55.0%
!! GAGAGGGGTGTCTTTGGAAG+TGG - chr4_1:46255305-46255324 None:intergenic 55.0%
CTGCAACACAACCGCAGTAG+AGG + chr4_1:46255429-46255448 Msa0550730:intron 55.0%
GGCTGATACTGCCTCTACTG+CGG - chr4_1:46255443-46255462 None:intergenic 55.0%
! GGGTGTCTTTGGAAGTGGGG+TGG - chr4_1:46255300-46255319 None:intergenic 60.0%
! GGGTGTCTTTGGAAGTGGGG+TGG - chr4_1:46255300-46255319 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 46254774 46256528 46254774 ID=Msa0550730;Name=Msa0550730
chr4_1 mRNA 46254774 46256528 46254774 ID=Msa0550730-mRNA-1;Parent=Msa0550730;Name=Msa0550730-mRNA-1;_AED=0.05;_eAED=0.05;_QI=380|1|1|1|1|1|3|168|259
chr4_1 exon 46254774 46255339 46254774 ID=Msa0550730-mRNA-1:exon:12325;Parent=Msa0550730-mRNA-1
chr4_1 exon 46255634 46256008 46255634 ID=Msa0550730-mRNA-1:exon:12326;Parent=Msa0550730-mRNA-1
chr4_1 exon 46256142 46256528 46256142 ID=Msa0550730-mRNA-1:exon:12327;Parent=Msa0550730-mRNA-1
chr4_1 five_prime_UTR 46254774 46255153 46254774 ID=Msa0550730-mRNA-1:five_prime_utr;Parent=Msa0550730-mRNA-1
chr4_1 CDS 46255154 46255339 46255154 ID=Msa0550730-mRNA-1:cds;Parent=Msa0550730-mRNA-1
chr4_1 CDS 46255634 46256008 46255634 ID=Msa0550730-mRNA-1:cds;Parent=Msa0550730-mRNA-1
chr4_1 CDS 46256142 46256360 46256142 ID=Msa0550730-mRNA-1:cds;Parent=Msa0550730-mRNA-1
chr4_1 three_prime_UTR 46256361 46256528 46256361 ID=Msa0550730-mRNA-1:three_prime_utr;Parent=Msa0550730-mRNA-1
Gene Sequence

>Msa0550730

ATGAAGATCCAGTGTGATGCATGTCACAAACAAGAGGCCTCCTTGTTCTGCCCTGCAGATGAAGCAGCTCTCTGCAATCAATGTGATCGCAATATCCACTATGCCAACAAGGTCTCTGCTAAACACAAACGCTTCACTCTTCACCACCCCACTTCCAAAGACACCCCTCTCTGTGATATCTGCAAGGAGAGGCGTGCATATCTATTTTGCAAAGAAGATAGAGCAATACTTTGCAGGGAATGTGACATTCCTATCCATGAAGTCAACAAACTTACCAAGCAACACAACAGGTTTCTTCTCACCGGCGTAAAGATCGGTGCTTGTTCTTCTTATTCAAACCCAACAACTTCCAATGGCTCAGAACTAAGAACCTCAAGTCCAAGACCAAGTTCATTTTCAAGTGAAAATAATAGTTGTTCTCAAAGTTCTTTCAAAGAAAACATGGCTTGTGACACAGTTTCAACCAGTAGCATTTCCGAATACTTGATTGAAACAATTCCTGGTTACTGCATGGAAGACCTTTTTGATGCTTCTTTTACACCTAATAACGTTTTCTGTAAGGATTATCTTGAACAGAACCAAGATCTTCAAGTTATAAACATGTCTGACTGGGTACCACAATCTCAAGTTAGATTCCCTCAGCTTAGCGCTAATTCGAATGTTCCCCACATTGATTCTTTAGACGGGGTTATGAAAATGCAACATACTAAAGCTGTTGAAGGATACAAAGTTCCTTCAATTAGTCCTCCATTCTTCAAACAATGTACAGGCACTCGTTAA
Protein sequence

>Msa0550730

MKIQCDACHKQEASLFCPADEAALCNQCDRNIHYANKVSAKHKRFTLHHPTSKDTPLCDICKERRAYLFCKEDRAILCRECDIPIHEVNKLTKQHNRFLLTGVKIGACSSYSNPTTSNGSELRTSSPRPSSFSSENNSCSQSSFKENMACDTVSTSSISEYLIETIPGYCMEDLFDASFTPNNVFCKDYLEQNQDLQVINMSDWVPQSQVRFPQLSANSNVPHIDSLDGVMKMQHTKAVEGYKVPSISPPFFKQCTGTR*