AlfalfaGEDB | Msa1349500

M. sativa cultivar zhongmu-4 / Msa1349500

KEGG: K01179
GO: -
PFAM: PF00759.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1349500	XP_003611061.1	82.973	370	42	1	21	369	20	389	0.0	588
Msa1349500	XP_003611061.1	93.519	432	27	1	650	1080	73	504	0.0	790

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1349500	sp\|P22503\|GUN_PHAVU	81.840	424	76	1	650	1072	73	496	0.0	723
Msa1349500	sp\|P22503\|GUN_PHAVU	74.169	391	79	2	1	370	1	390	0.0	579

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1349500	G7KC03	82.973	370	42	1	21	369	20	389	0.0	588
Msa1349500	G7KC03	93.519	432	27	1	650	1080	73	504	0.0	790

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0389900	Msa1349500	0.892180	2.012832e-74	-8.615850e-47
Msa0712570	Msa1349500	0.815360	9.623792e-52	-8.615850e-47
Msa0752960	Msa1349500	0.856867	2.405961e-62	-8.615850e-47
Msa0787790	Msa1349500	0.803197	3.910557e-49	-8.615850e-47
Msa0863990	Msa1349500	0.817756	2.796235e-52	-8.615850e-47
Msa1058320	Msa1349500	0.804597	2.001134e-49	-8.615850e-47
Msa1101240	Msa1349500	0.802500	5.447352e-49	-8.615850e-47
Msa1349500	Msa1407880	0.818886	1.551115e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1349500	MtrunA17_Chr5g0397201	93.519	432	27	1	650	1080	73	504	0.0	790
Msa1349500	MtrunA17_Chr5g0397201	82.973	370	42	1	21	369	20	389	0.0	588
Msa1349500	MtrunA17_Chr5g0397181	84.788	401	60	1	678	1077	1	401	0.0	687
Msa1349500	MtrunA17_Chr5g0397181	89.965	289	8	1	102	369	1	289	4.54e-169	502
Msa1349500	MtrunA17_Chr5g0397191	82.997	347	4	2	367	658	26	372	0.0	566
Msa1349500	MtrunA17_Chr3g0119821	52.817	426	188	6	659	1074	77	499	1.10e-148	453
Msa1349500	MtrunA17_Chr3g0119821	49.046	367	145	8	38	369	32	391	9.40e-105	337
Msa1349500	MtrunA17_Chr1g0195271	51.551	419	191	5	659	1068	81	496	4.98e-143	439
Msa1349500	MtrunA17_Chr1g0195271	48.619	362	153	7	38	369	36	394	5.20e-100	325
Msa1349500	MtrunA17_Chr3g0145261	49.052	422	202	5	659	1069	66	485	1.06e-136	421

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1349500	AT4G23560.1	66.667	462	147	2	610	1069	19	475	0.0	612
Msa1349500	AT4G23560.1	67.622	349	92	1	42	369	24	372	3.60e-151	459
Msa1349500	AT4G09740.1	70.803	411	120	0	659	1069	65	475	0.0	601
Msa1349500	AT4G09740.1	58.894	416	143	3	23	410	5	420	1.31e-149	455
Msa1349500	AT1G63990.1	70.418	311	71	1	368	657	28	338	4.69e-149	450
Msa1349500	AT1G02800.1	53.682	421	181	6	657	1068	83	498	1.58e-147	451
Msa1349500	AT1G02800.1	46.384	401	178	8	1	369	1	396	2.73e-101	328
Msa1349500	AT4G02290.1	50.597	419	195	5	657	1066	92	507	1.19e-141	436
Msa1349500	AT4G02290.1	47.790	362	156	6	38	369	49	407	3.77e-99	323
Msa1349500	AT1G23210.1	50.120	415	193	6	659	1063	70	480	3.43e-139	428

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Find 238 sgRNAs with CRISPR-Local

Find 715 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATTGATCAGGCAGCA+TGG	0.565462	tig0010965:+9133	None:intergenic
AAAAGCAGCATTCATATAGG+TGG	0.619409	tig0010965:+5294	None:intergenic
AAACGCAGATCATAATTGTT+GGG	0.523983	tig0010965:-6918	Msa1349500:CDS
AAACGCAGATCATCAATGTT+GGG	0.510319	tig0010965:-16590	Msa1349500:CDS
AAAGATTGATCAGGCAGCAT+GGG	0.554309	tig0010965:+9134	None:intergenic
AAAGGCTCTAATTTCAGAGG+GGG	0.637646	tig0010965:+5675	None:intergenic
AAATATAGAGGTTCTTACTC+GGG	0.568349	tig0010965:-6480	Msa1349500:CDS
AAATTTGGAAGTGTAGGAAT+GGG	0.504401	tig0010965:-9350	Msa1349500:CDS
AACAAAAGCAGCATTCATAT+AGG	0.500246	tig0010965:+5291	None:intergenic
AACTATTATGGGCTGCTTCT+TGG	0.270350	tig0010965:-6313	Msa1349500:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCATTATTCTTACTACTCA+TGG	+	tig0010965:5280-5299	None:intergenic	30.0%
TAATGGTCTATTAGCCATAA+TGG	-	tig0010965:5293-5312	Msa1349500:CDS	30.0%
TTGTCCTTCAAAAAACAAGA+TGG	+	tig0010965:5382-5401	None:intergenic	30.0%
TCAAGAAATCTATCAAAAGG+GGG	-	tig0010965:5708-5727	Msa1349500:CDS	30.0%
TCTATAGTTACACATATGAG+TGG	+	tig0010965:5842-5861	None:intergenic	30.0%
TTGAATGTTATTGCATGAGA+GGG	-	tig0010965:6029-6048	Msa1349500:CDS	30.0%
TAATACACATGATCAGTCAT+AGG	+	tig0010965:6157-6176	None:intergenic	30.0%
GTGTATTATATATCTTTGGC+AGG	-	tig0010965:6169-6188	Msa1349500:intron	30.0%
CATGATATATAGGGTAAGTT+TGG	+	tig0010965:6596-6615	None:intergenic	30.0%
AATATTTAACCTGCAACACA+AGG	+	tig0010965:6666-6685	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0010965	gene	5234	18111	5234	ID=Msa1349500;Name=Msa1349500
tig0010965	mRNA	5234	18111	5234	ID=Msa1349500-mRNA-1;Parent=Msa1349500;Name=Msa1349500-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0\|0.28\|0.13\|1\|0.85\|0.90\|22\|0\|1080
tig0010965	exon	17866	18111	17866	ID=Msa1349500-mRNA-1:exon:6978;Parent=Msa1349500-mRNA-1
tig0010965	exon	16995	17154	16995	ID=Msa1349500-mRNA-1:exon:6977;Parent=Msa1349500-mRNA-1
tig0010965	exon	16423	16613	16423	ID=Msa1349500-mRNA-1:exon:6976;Parent=Msa1349500-mRNA-1
tig0010965	exon	16262	16339	16262	ID=Msa1349500-mRNA-1:exon:6975;Parent=Msa1349500-mRNA-1
tig0010965	exon	16004	16156	16004	ID=Msa1349500-mRNA-1:exon:6974;Parent=Msa1349500-mRNA-1
tig0010965	exon	15775	15883	15775	ID=Msa1349500-mRNA-1:exon:6973;Parent=Msa1349500-mRNA-1
tig0010965	exon	15504	15670	15504	ID=Msa1349500-mRNA-1:exon:6972;Parent=Msa1349500-mRNA-1
tig0010965	exon	11994	12077	11994	ID=Msa1349500-mRNA-1:exon:6971;Parent=Msa1349500-mRNA-1
tig0010965	exon	10981	11110	10981	ID=Msa1349500-mRNA-1:exon:6970;Parent=Msa1349500-mRNA-1
tig0010965	exon	10754	10891	10754	ID=Msa1349500-mRNA-1:exon:6969;Parent=Msa1349500-mRNA-1
tig0010965	exon	10567	10655	10567	ID=Msa1349500-mRNA-1:exon:6968;Parent=Msa1349500-mRNA-1
tig0010965	exon	9992	10120	9992	ID=Msa1349500-mRNA-1:exon:6967;Parent=Msa1349500-mRNA-1
tig0010965	exon	9783	9877	9783	ID=Msa1349500-mRNA-1:exon:6966;Parent=Msa1349500-mRNA-1
tig0010965	exon	9330	9406	9330	ID=Msa1349500-mRNA-1:exon:6965;Parent=Msa1349500-mRNA-1
tig0010965	exon	9070	9197	9070	ID=Msa1349500-mRNA-1:exon:6964;Parent=Msa1349500-mRNA-1
tig0010965	exon	7075	7287	7075	ID=Msa1349500-mRNA-1:exon:6963;Parent=Msa1349500-mRNA-1
tig0010965	exon	6751	6951	6751	ID=Msa1349500-mRNA-1:exon:6962;Parent=Msa1349500-mRNA-1
tig0010965	exon	6443	6520	6443	ID=Msa1349500-mRNA-1:exon:6961;Parent=Msa1349500-mRNA-1
tig0010965	exon	6187	6339	6187	ID=Msa1349500-mRNA-1:exon:6960;Parent=Msa1349500-mRNA-1
tig0010965	exon	5949	6057	5949	ID=Msa1349500-mRNA-1:exon:6959;Parent=Msa1349500-mRNA-1
tig0010965	exon	5663	5829	5663	ID=Msa1349500-mRNA-1:exon:6958;Parent=Msa1349500-mRNA-1
tig0010965	exon	5234	5581	5234	ID=Msa1349500-mRNA-1:exon:6957;Parent=Msa1349500-mRNA-1
tig0010965	CDS	17866	18111	17866	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	16995	17154	16995	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	16423	16613	16423	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	16262	16339	16262	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	16004	16156	16004	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	15775	15883	15775	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	15504	15670	15504	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	11994	12077	11994	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	10981	11110	10981	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	10754	10891	10754	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	10567	10655	10567	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	9992	10120	9992	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	9783	9877	9783	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	9330	9406	9330	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	9070	9197	9070	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	7075	7287	7075	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	6751	6951	6751	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	6443	6520	6443	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	6187	6339	6187	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	5949	6057	5949	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	5663	5829	5663	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1
tig0010965	CDS	5234	5581	5234	ID=Msa1349500-mRNA-1:cds;Parent=Msa1349500-mRNA-1

Gene Sequence

>Msa1349500

ATGGGGTTCTATTTGTTGATAAGTGGTTTGATCTTGTGGTTTGCCATGAGTAGTAAGAATAATGGTCTATTAGCCATAATGGATGAGGAATTAATTAGTAAAAGTTCCTCATCTAGTTACAACTATGCAGATGCTCTTGGCAAAGCCATCTTGTTTTTTGAAGGACAACGCTCAGGGAAATTGCCTAAAGACCAAAGAGTGAAGTGGAGGGGAGACTCAGCTCTCTCTGATGGCAAAACTCAAAATGTGAATCTGGTTGGAGGATACTATGATGCTGGTGATAATGTGAAGTTTGGATGGCCAATGTCATTTACAGTCAGCTTATTGAGTTGGGCTGCTGTTGAATATGAAAGTGAGATTTCTTCAGTTAACCAGATTGATTATCTCCGCCGTGCTATTCGTTGGGGAAACGCAGATCATCAATGTTGGGAGCGTCCGGAAGACATGGATACTCCAAGAACACTTTACAAGATTGATGCTAATTCCCCTGGAACTGAGGCTGCAGCTGAGGCTGCTGCTGCACTTTCAGCTGCTTCAATTGTCTTTAAGAAAAAAGACACTCAATATTCATCGAAGTTGTTAAGACATTCAAAATCTTTGTTTGATTTTGCTGACAAGTATAGAGGCACCTACACGGGTTCTTGTCCATTCTACTGCTCGTACTCTGGTTACCAAGATGAACTGTTATGGGCTGCTGCTTGGTTATACAAGGCCAGTGGAGAAAGCAAGTACTTGAAGTACATCACAGATAACCAAGGTTGGAATCAGGCAGCGTCTGAGTTCAGCTGGGACAACAAATTTGTTGGAGTTCAAACATTACTAACACAGGAATTCTATGGTGGGAAGAAGGACTTAGCCAAGATTCATAGTGATGGTGAATCATTTATATGTGCATTGATGCCAGGAAGTTACTCTCTACAGATCAAAACAACTCCTGGTGGACTTTTGTATACTAGAGATAGTAGTAATTTGCAATACACCACAACCTCAACCATGGTACTATTCATTTTCTCCAAAATCCTAAATAAAAATCATATTGATGGAATCCATTGTGGCTCTACCAATTTCACATCCTCTGAAATCAGAGCCTTTGCAAAATCACAGGTTCGAGCAAGAATTGAAGTTTCAGTTCTCAGTTTCCTCAAAATATTGAATGCGTCAAACCCTGCTATATCAGATTTGCCTCTGATTCAAAGAAAATCGAGCAACAGTCGAGTGAATCATGGCCTGTTGACCGAATTATCATATGTTTTTCTCTCTGATTCCATATCTACAAGGTCTCTTATGAGACCTAATGCAGCAAAAGCCTTTGTTAGGGTGTGGAAGGTGATGGAAATGTGCTATCAGATATTGCTTCAGGAAAAGCGTGTCACACAGAGGGAACTCTTCTACAAGCTGCTGTGTGATTCACAACATCTGTTCCCTTCTCAAAAGGACGTCAATAGGACAATCCAAGATCTTGTAGCGTTGCTTCGGTGCAGCAGATACAGTCTTGGAATCATGGCATCTAGCCGAGGACTCATTGCTGGACGTCTGATTTTGCAGGAACCGGGCAAAGAGGCTGTTGATTGCTCTGTGTGTGGATCTTCTGGACATGCAATTTCTGGTGACTTGAATTTGTTAGATAAATTGATTCTAAATGCAGATGCACGTTACATTATAATTGTGGAGAAGCATGCAATATTTCAACGACTTGCTGAAGATAGGTTTTTTAATCAAATTCCAAGCATTCTTATCACAGCTAAAGGTTACCCAGATATTGCCACAAGGAATCCAGCTGGATTAGCTATACTATGCACCTTCAAATTTGGAAGTGTAGGAATGGGCCTAGAGTCATACAGATACGCTTGCAATGTCAAGTGGTTAGGACTACGGGGGCATGATCTACCCATGCTGCCTGATCAATCTTTTGTTCCATTGAAGACAAAGGACCTACAAATTGCTCAAAGCTTGATGTCCTCGAGAATCTTACCGGTGGATTTGGTTGGAGGATACTACGACGCGGGTGATAATGTGAAGTTTGGTTGGCCGATGTCTTTTACGGTGAGCTTGTTGAGTTGGACTGCTGTTGAATATGAAAGTGAGATTTCTTCGGTGAACCAGCTTGATTATCTTCGCAGTGCTATTCGTTGGGGTGCAGATTTCATACTTAAAGCTCACACTTCACCAACCACGCTCTTTACACAGGTGGGAGATGGAAACGCAGATCATAATTGTTGGGAGCGTCCAGAAGACATGGATACACCAAGAACAACTTACAAGATTGATTCGAATTCCCCTGGAACTGAGGCTGCAGCTGAGGCTGCTGCCGCTCTTTCTGCTGCTTCAATTGTCTTTAAGAAAACAGATATCAATTATTCATCCAAGCTATTAAGCCAGTCAAAATCTTTGTTTGATTTTGCTGACAAATATAGAGGTTCTTACTCGGGTTCTTGCCCATTTTACTGCTCGTACTCTGGTTATCAGGATGAACTATTATGGGCTGCTTCTTGGCTATACAAGGCTAGTGGGGAAAGCAAGTACTTGACGTACATCACAAGTAACCAAGGTTGGAGTCAAGCAGTGTCTGAATTCAGCTGGGATAACAAATTTGTTGGAGCTCAAACATTACTAACACAGGAATTCTATGGCGGGAAGAAGGAGTTAGCCAAGATTAAGACTGATGGTGAATCATTTATATGTGCATTGATGCCAGGAAGTAGCTCTCTACAGATTAGAACAACTCCAGGTGGGCTTCTGTATATTAGAGATGGTAGTAATTTGCAATATACCACAACCTCAACCATGGTGCTATTCATTTTCTCCAAAATCTTAAATAAAAATCATATTGATGGAATTCAGTGTGGCTCTGCCCATTTCTCCCCCTCTGAAATTAGAGCCTTTGCAAAATCACAGGTGGACTACATATTAGGAAACAATCCCATGAAGATGTCATACATGGTGGGATATGGGAGTAAATATCCAAAACAATTACATCACAGAGGTTCTTCCATCCCTTCAATCATATCTCAACCTGCCAAAGTGGGGTGCAATGATGGCCAGAACTATTTCTCTTCATCTAATCCAAATCCTAATATTCATGTGGGTGCTATTGTTGGAGGACCAAATTCTAATGACCAATACAATGATGCAAGATCTGATTACTCTCATGCAGAACCTACCACCTATATGAATGCTGCTTTTGTTGGTTCTGTTGCAGCTTTGCTTGGTGAAACTAACACCGAGACTCTTCAATTTTCTTGA

Protein sequence

>Msa1349500

MGFYLLISGLILWFAMSSKNNGLLAIMDEELISKSSSSSYNYADALGKAILFFEGQRSGKLPKDQRVKWRGDSALSDGKTQNVNLVGGYYDAGDNVKFGWPMSFTVSLLSWAAVEYESEISSVNQIDYLRRAIRWGNADHQCWERPEDMDTPRTLYKIDANSPGTEAAAEAAAALSAASIVFKKKDTQYSSKLLRHSKSLFDFADKYRGTYTGSCPFYCSYSGYQDELLWAAAWLYKASGESKYLKYITDNQGWNQAASEFSWDNKFVGVQTLLTQEFYGGKKDLAKIHSDGESFICALMPGSYSLQIKTTPGGLLYTRDSSNLQYTTTSTMVLFIFSKILNKNHIDGIHCGSTNFTSSEIRAFAKSQVRARIEVSVLSFLKILNASNPAISDLPLIQRKSSNSRVNHGLLTELSYVFLSDSISTRSLMRPNAAKAFVRVWKVMEMCYQILLQEKRVTQRELFYKLLCDSQHLFPSQKDVNRTIQDLVALLRCSRYSLGIMASSRGLIAGRLILQEPGKEAVDCSVCGSSGHAISGDLNLLDKLILNADARYIIIVEKHAIFQRLAEDRFFNQIPSILITAKGYPDIATRNPAGLAILCTFKFGSVGMGLESYRYACNVKWLGLRGHDLPMLPDQSFVPLKTKDLQIAQSLMSSRILPVDLVGGYYDAGDNVKFGWPMSFTVSLLSWTAVEYESEISSVNQLDYLRSAIRWGADFILKAHTSPTTLFTQVGDGNADHNCWERPEDMDTPRTTYKIDSNSPGTEAAAEAAAALSAASIVFKKTDINYSSKLLSQSKSLFDFADKYRGSYSGSCPFYCSYSGYQDELLWAASWLYKASGESKYLTYITSNQGWSQAVSEFSWDNKFVGAQTLLTQEFYGGKKELAKIKTDGESFICALMPGSSSLQIRTTPGGLLYIRDGSNLQYTTTSTMVLFIFSKILNKNHIDGIQCGSAHFSPSEIRAFAKSQVDYILGNNPMKMSYMVGYGSKYPKQLHHRGSSIPSIISQPAKVGCNDGQNYFSSSNPNPNIHVGAIVGGPNSNDQYNDARSDYSHAEPTTYMNAAFVGSVAALLGETNTETLQFS*