AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1349600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1349600 MtrunA17_Chr5g0397281 90.367 218 21 0 1 218 1 218 1.04e-146 410
Msa1349600 MtrunA17_Chr3g0126191 33.824 136 90 0 73 208 76 211 2.46e-24 97.1
Msa1349600 MtrunA17_Chr7g0221181 30.827 133 92 0 81 213 76 208 2.11e-23 94.0
Msa1349600 MtrunA17_Chr2g0289851 23.762 202 134 6 19 201 13 213 3.22e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1349600 AT5G41470.1 48.148 135 70 0 74 208 66 200 8.99e-38 133
Msa1349600 AT1G71480.1 32.576 132 89 0 81 212 69 200 9.00e-26 100
Msa1349600 AT1G71480.3 33.333 105 70 0 81 185 69 173 5.26e-23 92.0
Msa1349600 AT1G71480.2 28.916 83 59 0 81 163 69 151 2.31e-12 63.2

Find 80 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAACTCTGGTTGGGAATT+TGG 0.130689 tig0010967:+57405 Msa1349620:intron
CCACCACTTCCTCCTGTTAT+AGG 0.246729 tig0010967:+55998 Msa1349610:CDS
TGACTATGAATTTCACATTC+TGG 0.276043 tig0010967:+56901 Msa1349620:intron
CAAACCTTCTTTCCTTGAAA+TGG 0.293968 tig0010967:+57061 Msa1349620:intron
TGGCATGGTAGAATGGAAAA+AGG 0.312351 tig0010967:-56737 Msa1349600:intron
TAGAAGAAAACATGACAATT+AGG 0.322961 tig0010967:-56666 Msa1349600:intron
GACCTCATGCAATACTATTA+TGG 0.322975 tig0010967:-56250 Msa1349600:three_prime_UTR
TTGAGTTTATTCCCGGATAA+TGG 0.328554 tig0010967:+57380 Msa1349620:intron
ATTTAACTGTTGCGGCAAAA+TGG 0.329724 tig0010967:-56857 Msa1349600:CDS
TCCTCCTGTTATAGGATCAT+TGG 0.342440 tig0010967:+56006 Msa1349610:CDS
TTGATGTTTGAGTTTATTCC+CGG 0.352803 tig0010967:+57373 Msa1349620:intron
GCTTGTGCTTCAGAAATCTA+AGG 0.355699 tig0010967:+55550 None:intergenic
GACTCACATTCAGCTGCATT+TGG 0.356576 tig0010967:+56358 Msa1349620:intron
GTTACCAAAACTATGCTTCC+AGG 0.364460 tig0010967:+56534 Msa1349620:intron
TTACAACACTTGAAAGAGTT+AGG 0.368447 tig0010967:+56498 Msa1349620:intron
CCCTCTCGTTGTTGGATACA+TGG 0.370228 tig0010967:+56032 Msa1349610:CDS
CAGAGTTTCATGCCATTATC+CGG 0.370820 tig0010967:-57392 Msa1349600:CDS
GGCCGGCTATAGGCGACTAT+TGG 0.377649 tig0010967:-55974 Msa1349600:three_prime_UTR
AGCTATCTAGTAGTGGATTA+AGG 0.384056 tig0010967:+56192 None:intergenic
ACCTTCATCAATCCATTTCA+AGG 0.398951 tig0010967:-57073 Msa1349600:CDS
ATTGGTTGCCCTCTCGTTGT+TGG 0.399335 tig0010967:+56024 Msa1349610:CDS
CAAACCTGGAAGCATAGTTT+TGG 0.405840 tig0010967:-56538 Msa1349600:CDS
CAATCCATTTCAAGGAAAGA+AGG 0.408899 tig0010967:-57065 Msa1349600:intron
AAGTGGTGGAGGCCGGCTAT+AGG 0.418164 tig0010967:-55984 Msa1349600:three_prime_UTR
TTCTTACAGCAGCTTACTGC+TGG 0.418688 tig0010967:-56930 Msa1349600:CDS
AGGAACATGCATGTATGTTA+AGG 0.419513 tig0010967:+56289 None:intergenic
TATAGTTACTCACCTGCAAT+TGG 0.426851 tig0010967:-55584 Msa1349600:three_prime_UTR
AAAGATGAATCTGGCGAGAT+GGG 0.432844 tig0010967:+55926 Msa1349610:CDS
CGGATAATGGCATGAAACTC+TGG 0.438289 tig0010967:+57393 Msa1349620:intron
GCAACCAATGATCCTATAAC+AGG 0.441435 tig0010967:-56010 Msa1349600:three_prime_UTR
TACGTGTCTGTATCGTGTCT+TGG 0.447616 tig0010967:+55491 None:intergenic
ACAGCAGCTTACTGCTGGCA+TGG 0.459854 tig0010967:-56925 Msa1349600:CDS
GCCGGCTATAGGCGACTATT+GGG 0.460498 tig0010967:-55973 Msa1349600:three_prime_UTR
ACATACATGCATGTTCCTGC+AGG 0.462917 tig0010967:-56284 Msa1349600:three_prime_UTR
TCCTTGAAATGGATTGATGA+AGG 0.467392 tig0010967:+57072 Msa1349620:intron
ACCAATTGAATCAATCCAAA+AGG 0.487395 tig0010967:+56441 Msa1349620:intron
GATCCATTCACTTGGTAGAC+CGG 0.491216 tig0010967:-55676 Msa1349600:three_prime_UTR
GGAACATGCATGTATGTTAA+GGG 0.494614 tig0010967:+56290 None:intergenic
GAACAAATCCCATTCACAAC+AGG 0.495206 tig0010967:-56715 Msa1349600:CDS
AAGTGATCTACTATCTCTGC+TGG 0.496660 tig0010967:+57172 Msa1349620:intron
AGCTGCAGCCTGTTGTGAAT+GGG 0.499013 tig0010967:+56707 Msa1349620:intron
AAGCTGCAGCCTGTTGTGAA+TGG 0.506105 tig0010967:+56706 Msa1349620:intron
AAGCCGGTCTACCAAGTGAA+TGG 0.508284 tig0010967:+55673 Msa1349610:CDS
CTAAGACTATATTTAAGTAG+AGG 0.515504 tig0010967:-56103 Msa1349600:three_prime_UTR
AATGGCATGAAACTCTGGTT+GGG 0.526617 tig0010967:+57398 Msa1349620:intron
AAAAGATGAATCTGGCGAGA+TGG 0.530203 tig0010967:+55925 Msa1349610:CDS
GATCCTATAACAGGAGGAAG+TGG 0.536218 tig0010967:-56001 Msa1349600:three_prime_UTR
CAGGAGGAAGTGGTGGAGGC+CGG 0.536906 tig0010967:-55991 Msa1349600:three_prime_UTR
CAGCAGCTTACTGCTGGCAT+GGG 0.537521 tig0010967:-56924 Msa1349600:CDS
TAATGGCATGAAACTCTGGT+TGG 0.544699 tig0010967:+57397 Msa1349620:intron
AGAGTTTCATGCCATTATCC+GGG 0.545323 tig0010967:-57391 Msa1349600:CDS
ACCCAATAGTCGCCTATAGC+CGG 0.547078 tig0010967:+55972 Msa1349610:CDS
GGGAGATGATTTAACTGTTG+CGG 0.550185 tig0010967:-56865 Msa1349600:CDS
CTACCAAGTGAATGGATCAC+AGG 0.559874 tig0010967:+55681 Msa1349610:CDS
AGAAACATTACAAATGGATG+TGG 0.563651 tig0010967:-57431 None:intergenic
TGCTGTCCTCATCAGTGCTG+CGG 0.577568 tig0010967:-56060 Msa1349600:three_prime_UTR
GTCATCAAACAATGAAGTCA+CGG 0.578442 tig0010967:+56384 Msa1349620:intron
TAGATAGCTACATAAAGCTA+TGG 0.579049 tig0010967:-56178 Msa1349600:three_prime_UTR
ATAACAGGAGGAAGTGGTGG+AGG 0.586799 tig0010967:-55995 Msa1349600:three_prime_UTR
ATTGAATCACTAGTCAAACC+TGG 0.588394 tig0010967:-56552 Msa1349600:CDS
CCTATAACAGGAGGAAGTGG+TGG 0.596015 tig0010967:-55998 Msa1349600:three_prime_UTR
GTGGAACCGCAGCACTGATG+AGG 0.597213 tig0010967:+56054 Msa1349610:CDS
GTTGCGGCAAAATGGCACTT+AGG 0.602225 tig0010967:-56849 Msa1349600:intron
GGTCTTCTTAAGAACCCTGC+AGG 0.602800 tig0010967:+56269 None:intergenic
TCTAGTAGTGGATTAAGGAA+AGG 0.610402 tig0010967:+56197 None:intergenic
TTTATGTAGCTATCTAGTAG+TGG 0.616283 tig0010967:+56185 None:intergenic
TACCCTGTGATCCATTCACT+TGG 0.621091 tig0010967:-55684 Msa1349600:three_prime_UTR
TTTGCTAGGCGAAAAGCTGA+GGG 0.630682 tig0010967:+57339 Msa1349620:intron
CATACATGCATGTTCCTGCA+GGG 0.632925 tig0010967:-56283 Msa1349600:three_prime_UTR
ATAGTCAGAAATATATGCGA+GGG 0.636276 tig0010967:-56885 Msa1349600:CDS
AAGATGAATCTGGCGAGATG+GGG 0.637726 tig0010967:+55927 Msa1349610:CDS
ATCCATAATAGTATTGCATG+AGG 0.639529 tig0010967:+56248 None:intergenic
CATAGTCAGAAATATATGCG+AGG 0.642978 tig0010967:-56886 Msa1349600:CDS
ACCAATGATCCTATAACAGG+AGG 0.648824 tig0010967:-56007 Msa1349600:three_prime_UTR
ACCATGTATCCAACAACGAG+AGG 0.650347 tig0010967:-56033 Msa1349600:three_prime_UTR
CCATGTATCCAACAACGAGA+GGG 0.655825 tig0010967:-56032 Msa1349600:three_prime_UTR
AGCTGAATGTGAGTCAAACA+TGG 0.656790 tig0010967:-56350 Msa1349600:three_prime_UTR
CTATAGATAAAGTTCATGCG+TGG 0.662656 tig0010967:+55610 None:intergenic
TACCAAGTGAATGGATCACA+GGG 0.666568 tig0010967:+55682 Msa1349610:CDS
TCTCGTTGTTGGATACATGG+TGG 0.695822 tig0010967:+56035 Msa1349610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCTTATATCATTTATACTTT+AGG + tig0010967:56117-56136 None:intergenic 20.0%
!!! TTTCTTCTACTTTTTCTAAT+TGG + tig0010967:56213-56232 None:intergenic 20.0%
!!! ATGATATTTTGCATTACTAT+AGG + tig0010967:57015-57034 Msa1349620:intron 20.0%
!! GCTTATATCATTTATACTTT+AGG + tig0010967:56117-56136 None:intergenic 20.0%
!!! TTTCTTCTACTTTTTCTAAT+TGG + tig0010967:56213-56232 None:intergenic 20.0%
!!! ATGATATTTTGCATTACTAT+AGG + tig0010967:57015-57034 Msa1349620:intron 20.0%
!! TTTGAATTTTTCCCGAATTT+GGG + tig0010967:55694-55713 Msa1349610:intron 25.0%
! TGAAAAAGAGCTAAAACAAT+TGG - tig0010967:55756-55775 Msa1349600:intron 25.0%
! TAATCTAAACATAACATTGC+AGG - tig0010967:55926-55945 Msa1349600:three_prime_UTR 25.0%
! AAAAGCAATAAATCTAGGAA+AGG + tig0010967:56074-56093 None:intergenic 25.0%
! TAGAAGAAAACATGACAATT+AGG - tig0010967:56224-56243 Msa1349600:three_prime_UTR 25.0%
! CTCACTATATAATTGAATTC+AGG - tig0010967:56300-56319 Msa1349600:three_prime_UTR 25.0%
! AAAGGAAACAAAAATACAGT+TGG + tig0010967:56434-56453 Msa1349620:intron 25.0%
!!! ACTGTATTTTTGTTTCCTTT+TGG - tig0010967:56434-56453 Msa1349600:three_prime_UTR 25.0%
!! GTATGTTAAGGGATATTTTT+GGG + tig0010967:56592-56611 Msa1349620:intron 25.0%
!! TGTATGTTAAGGGATATTTT+TGG + tig0010967:56593-56612 Msa1349620:intron 25.0%
! CTAAGACTATATTTAAGTAG+AGG - tig0010967:56787-56806 Msa1349600:intron 25.0%
! AAACAAGAAAAAGATGAATC+TGG + tig0010967:56976-56995 Msa1349620:intron 25.0%
!!! TCATCTTTTTCTTGTTTCTT+AGG - tig0010967:56979-56998 Msa1349600:intron 25.0%
! TTGCATTACTATAGGTATAT+TGG + tig0010967:57007-57026 Msa1349620:intron 25.0%
!! TTTGAATTTTTCCCGAATTT+GGG + tig0010967:55694-55713 Msa1349610:intron 25.0%
! TGAAAAAGAGCTAAAACAAT+TGG - tig0010967:55756-55775 Msa1349600:intron 25.0%
! TAATCTAAACATAACATTGC+AGG - tig0010967:55926-55945 Msa1349600:three_prime_UTR 25.0%
! AAAAGCAATAAATCTAGGAA+AGG + tig0010967:56074-56093 None:intergenic 25.0%
! TAGAAGAAAACATGACAATT+AGG - tig0010967:56224-56243 Msa1349600:three_prime_UTR 25.0%
! CTCACTATATAATTGAATTC+AGG - tig0010967:56300-56319 Msa1349600:three_prime_UTR 25.0%
! AAAGGAAACAAAAATACAGT+TGG + tig0010967:56434-56453 Msa1349620:intron 25.0%
!!! ACTGTATTTTTGTTTCCTTT+TGG - tig0010967:56434-56453 Msa1349600:three_prime_UTR 25.0%
!! GTATGTTAAGGGATATTTTT+GGG + tig0010967:56592-56611 Msa1349620:intron 25.0%
!! TGTATGTTAAGGGATATTTT+TGG + tig0010967:56593-56612 Msa1349620:intron 25.0%
! CTAAGACTATATTTAAGTAG+AGG - tig0010967:56787-56806 Msa1349600:intron 25.0%
! AAACAAGAAAAAGATGAATC+TGG + tig0010967:56976-56995 Msa1349620:intron 25.0%
!!! TCATCTTTTTCTTGTTTCTT+AGG - tig0010967:56979-56998 Msa1349600:intron 25.0%
! TTGCATTACTATAGGTATAT+TGG + tig0010967:57007-57026 Msa1349620:intron 25.0%
! TTGATGTTTGAGTTTATTCC+CGG + tig0010967:55520-55539 None:intergenic 30.0%
AGAATGTCAAAAACAGAACT+TGG - tig0010967:55629-55648 Msa1349600:three_prime_UTR 30.0%
GAATGTCAAAAACAGAACTT+GGG - tig0010967:55630-55649 Msa1349600:three_prime_UTR 30.0%
AATGTCAAAAACAGAACTTG+GGG - tig0010967:55631-55650 Msa1349600:three_prime_UTR 30.0%
TCAGCCATATTATCAAAAAG+AGG - tig0010967:55667-55686 Msa1349600:three_prime_UTR 30.0%
! TTGAATTTTTCCCGAATTTG+GGG + tig0010967:55693-55712 Msa1349610:intron 30.0%
! GTTTGAATTTTTCCCGAATT+TGG + tig0010967:55695-55714 Msa1349610:intron 30.0%
GTTATTAGAACTTAGAAGGA+TGG + tig0010967:55863-55882 Msa1349610:intron 30.0%
CTAGGTTATTAGAACTTAGA+AGG + tig0010967:55867-55886 Msa1349610:intron 30.0%
TGACTATGAATTTCACATTC+TGG + tig0010967:55992-56011 Msa1349610:CDS 30.0%
ATAGTCAGAAATATATGCGA+GGG - tig0010967:56005-56024 Msa1349600:three_prime_UTR 30.0%
AGCAGAAAAGCAATAAATCT+AGG + tig0010967:56079-56098 None:intergenic 30.0%
! AAAGAGTTAGGTTCTGTTTT+AGG + tig0010967:56383-56402 Msa1349620:intron 30.0%
TTACAACACTTGAAAGAGTT+AGG + tig0010967:56395-56414 Msa1349620:intron 30.0%
!! TCCTTTTGGATTGATTCAAT+TGG - tig0010967:56448-56467 Msa1349600:three_prime_UTR 30.0%
ACCAATTGAATCAATCCAAA+AGG + tig0010967:56452-56471 Msa1349620:intron 30.0%
ATCCATAATAGTATTGCATG+AGG + tig0010967:56645-56664 Msa1349620:intron 30.0%
TTTATGTAGCTATCTAGTAG+TGG + tig0010967:56708-56727 Msa1349620:intron 30.0%
TAGATAGCTACATAAAGCTA+TGG - tig0010967:56712-56731 Msa1349600:CDS 30.0%
!! TAGTTTGAGTGTTGAACAAT+GGG + tig0010967:57098-57117 Msa1349620:intron 30.0%
! TTGATGTTTGAGTTTATTCC+CGG + tig0010967:55520-55539 None:intergenic 30.0%
AGAATGTCAAAAACAGAACT+TGG - tig0010967:55629-55648 Msa1349600:three_prime_UTR 30.0%
GAATGTCAAAAACAGAACTT+GGG - tig0010967:55630-55649 Msa1349600:three_prime_UTR 30.0%
AATGTCAAAAACAGAACTTG+GGG - tig0010967:55631-55650 Msa1349600:three_prime_UTR 30.0%
TCAGCCATATTATCAAAAAG+AGG - tig0010967:55667-55686 Msa1349600:three_prime_UTR 30.0%
! TTGAATTTTTCCCGAATTTG+GGG + tig0010967:55693-55712 Msa1349610:intron 30.0%
! GTTTGAATTTTTCCCGAATT+TGG + tig0010967:55695-55714 Msa1349610:intron 30.0%
GTTATTAGAACTTAGAAGGA+TGG + tig0010967:55863-55882 Msa1349610:intron 30.0%
CTAGGTTATTAGAACTTAGA+AGG + tig0010967:55867-55886 Msa1349610:intron 30.0%
TGACTATGAATTTCACATTC+TGG + tig0010967:55992-56011 Msa1349610:CDS 30.0%
ATAGTCAGAAATATATGCGA+GGG - tig0010967:56005-56024 Msa1349600:three_prime_UTR 30.0%
AGCAGAAAAGCAATAAATCT+AGG + tig0010967:56079-56098 None:intergenic 30.0%
! AAAGAGTTAGGTTCTGTTTT+AGG + tig0010967:56383-56402 Msa1349620:intron 30.0%
TTACAACACTTGAAAGAGTT+AGG + tig0010967:56395-56414 Msa1349620:intron 30.0%
!! TCCTTTTGGATTGATTCAAT+TGG - tig0010967:56448-56467 Msa1349600:three_prime_UTR 30.0%
ACCAATTGAATCAATCCAAA+AGG + tig0010967:56452-56471 Msa1349620:intron 30.0%
ATCCATAATAGTATTGCATG+AGG + tig0010967:56645-56664 Msa1349620:intron 30.0%
TTTATGTAGCTATCTAGTAG+TGG + tig0010967:56708-56727 Msa1349620:intron 30.0%
TAGATAGCTACATAAAGCTA+TGG - tig0010967:56712-56731 Msa1349600:CDS 30.0%
!! TAGTTTGAGTGTTGAACAAT+GGG + tig0010967:57098-57117 Msa1349620:intron 30.0%
TTGAGTTTATTCCCGGATAA+TGG + tig0010967:55513-55532 None:intergenic 35.0%
! AAGTTGCATGATGTTTTGCT+AGG + tig0010967:55568-55587 None:intergenic 35.0%
ATGCAACTTGCACAAAAAGA+TGG - tig0010967:55579-55598 Msa1349600:three_prime_UTR 35.0%
ACCTTCATCAATCCATTTCA+AGG - tig0010967:55817-55836 Msa1349600:intron 35.0%
!! TCCTTGAAATGGATTGATGA+AGG + tig0010967:55821-55840 Msa1349610:intron 35.0%
CAATCCATTTCAAGGAAAGA+AGG - tig0010967:55825-55844 Msa1349600:intron 35.0%
CAAACCTTCTTTCCTTGAAA+TGG + tig0010967:55832-55851 Msa1349610:intron 35.0%
CATAGTCAGAAATATATGCG+AGG - tig0010967:56004-56023 Msa1349600:three_prime_UTR 35.0%
ATTTAACTGTTGCGGCAAAA+TGG - tig0010967:56033-56052 Msa1349600:three_prime_UTR 35.0%
! AAGCCTGACGAATATCTTTT+TGG - tig0010967:56133-56152 Msa1349600:three_prime_UTR 35.0%
ATGCCAAAAAGATATTCGTC+AGG + tig0010967:56139-56158 None:intergenic 35.0%
!! TGACGAATATCTTTTTGGCA+TGG - tig0010967:56138-56157 Msa1349600:three_prime_UTR 35.0%
!! ATCTTTTTGGCATGGTAGAA+TGG - tig0010967:56146-56165 Msa1349600:three_prime_UTR 35.0%
ATTGAATCACTAGTCAAACC+TGG - tig0010967:56338-56357 Msa1349600:three_prime_UTR 35.0%
GTCATCAAACAATGAAGTCA+CGG + tig0010967:56509-56528 Msa1349620:intron 35.0%
GGAACATGCATGTATGTTAA+GGG + tig0010967:56603-56622 Msa1349620:intron 35.0%
AGGAACATGCATGTATGTTA+AGG + tig0010967:56604-56623 Msa1349620:intron 35.0%
GACCTCATGCAATACTATTA+TGG - tig0010967:56640-56659 Msa1349600:intron 35.0%
!! TCTAGTAGTGGATTAAGGAA+AGG + tig0010967:56696-56715 Msa1349620:intron 35.0%
!! AGCTATCTAGTAGTGGATTA+AGG + tig0010967:56701-56720 Msa1349620:intron 35.0%
!! CTAGTTTGAGTGTTGAACAA+TGG + tig0010967:57099-57118 Msa1349620:intron 35.0%
TGCGAAAAACAGATTTAAGC+CGG + tig0010967:57236-57255 Msa1349620:intron 35.0%
CTATAGATAAAGTTCATGCG+TGG + tig0010967:57283-57302 Msa1349620:intron 35.0%
TATAGTTACTCACCTGCAAT+TGG - tig0010967:57306-57325 Msa1349600:CDS 35.0%
!!! GCTTGTTTTATTCCAATTGC+AGG + tig0010967:57321-57340 Msa1349620:intron 35.0%
AAGCACAAGCATGAAAAACT+CGG - tig0010967:57353-57372 Msa1349600:CDS 35.0%
TTGAGTTTATTCCCGGATAA+TGG + tig0010967:55513-55532 None:intergenic 35.0%
! AAGTTGCATGATGTTTTGCT+AGG + tig0010967:55568-55587 None:intergenic 35.0%
ATGCAACTTGCACAAAAAGA+TGG - tig0010967:55579-55598 Msa1349600:three_prime_UTR 35.0%
ACCTTCATCAATCCATTTCA+AGG - tig0010967:55817-55836 Msa1349600:intron 35.0%
!! TCCTTGAAATGGATTGATGA+AGG + tig0010967:55821-55840 Msa1349610:intron 35.0%
CAATCCATTTCAAGGAAAGA+AGG - tig0010967:55825-55844 Msa1349600:intron 35.0%
CAAACCTTCTTTCCTTGAAA+TGG + tig0010967:55832-55851 Msa1349610:intron 35.0%
CATAGTCAGAAATATATGCG+AGG - tig0010967:56004-56023 Msa1349600:three_prime_UTR 35.0%
ATTTAACTGTTGCGGCAAAA+TGG - tig0010967:56033-56052 Msa1349600:three_prime_UTR 35.0%
! AAGCCTGACGAATATCTTTT+TGG - tig0010967:56133-56152 Msa1349600:three_prime_UTR 35.0%
ATGCCAAAAAGATATTCGTC+AGG + tig0010967:56139-56158 None:intergenic 35.0%
!! TGACGAATATCTTTTTGGCA+TGG - tig0010967:56138-56157 Msa1349600:three_prime_UTR 35.0%
!! ATCTTTTTGGCATGGTAGAA+TGG - tig0010967:56146-56165 Msa1349600:three_prime_UTR 35.0%
ATTGAATCACTAGTCAAACC+TGG - tig0010967:56338-56357 Msa1349600:three_prime_UTR 35.0%
GTCATCAAACAATGAAGTCA+CGG + tig0010967:56509-56528 Msa1349620:intron 35.0%
GGAACATGCATGTATGTTAA+GGG + tig0010967:56603-56622 Msa1349620:intron 35.0%
AGGAACATGCATGTATGTTA+AGG + tig0010967:56604-56623 Msa1349620:intron 35.0%
GACCTCATGCAATACTATTA+TGG - tig0010967:56640-56659 Msa1349600:intron 35.0%
!! TCTAGTAGTGGATTAAGGAA+AGG + tig0010967:56696-56715 Msa1349620:intron 35.0%
!! AGCTATCTAGTAGTGGATTA+AGG + tig0010967:56701-56720 Msa1349620:intron 35.0%
!! CTAGTTTGAGTGTTGAACAA+TGG + tig0010967:57099-57118 Msa1349620:intron 35.0%
TGCGAAAAACAGATTTAAGC+CGG + tig0010967:57236-57255 Msa1349620:intron 35.0%
CTATAGATAAAGTTCATGCG+TGG + tig0010967:57283-57302 Msa1349620:intron 35.0%
TATAGTTACTCACCTGCAAT+TGG - tig0010967:57306-57325 Msa1349600:CDS 35.0%
!!! GCTTGTTTTATTCCAATTGC+AGG + tig0010967:57321-57340 Msa1349620:intron 35.0%
AAGCACAAGCATGAAAAACT+CGG - tig0010967:57353-57372 Msa1349600:CDS 35.0%
TGAAACTCTGGTTGGGAATT+TGG + tig0010967:55488-55507 None:intergenic 40.0%
AATGGCATGAAACTCTGGTT+GGG + tig0010967:55495-55514 None:intergenic 40.0%
TAATGGCATGAAACTCTGGT+TGG + tig0010967:55496-55515 None:intergenic 40.0%
CAGAGTTTCATGCCATTATC+CGG - tig0010967:55498-55517 Msa1349600:three_prime_UTR 40.0%
AGAGTTTCATGCCATTATCC+GGG - tig0010967:55499-55518 Msa1349600:three_prime_UTR 40.0%
!! GGGGCCTCTTTTTGATAATA+TGG + tig0010967:55674-55693 Msa1349610:CDS 40.0%
TCAAAAAGAGGCCCCAAATT+CGG - tig0010967:55679-55698 Msa1349600:three_prime_UTR 40.0%
AAGTGATCTACTATCTCTGC+TGG + tig0010967:55721-55740 Msa1349610:intron 40.0%
AGCTGTTTACTCGATGCAAT+CGG + tig0010967:55898-55917 Msa1349610:intron 40.0%
GGGAGATGATTTAACTGTTG+CGG - tig0010967:56025-56044 Msa1349600:three_prime_UTR 40.0%
TGGCATGGTAGAATGGAAAA+AGG - tig0010967:56153-56172 Msa1349600:three_prime_UTR 40.0%
GAACAAATCCCATTCACAAC+AGG - tig0010967:56175-56194 Msa1349600:three_prime_UTR 40.0%
!! CAAACCTGGAAGCATAGTTT+TGG - tig0010967:56352-56371 Msa1349600:three_prime_UTR 40.0%
GTTACCAAAACTATGCTTCC+AGG + tig0010967:56359-56378 Msa1349620:intron 40.0%
AGCTGAATGTGAGTCAAACA+TGG - tig0010967:56540-56559 Msa1349600:CDS 40.0%
GCAACCAATGATCCTATAAC+AGG - tig0010967:56880-56899 Msa1349600:CDS 40.0%
ACCAATGATCCTATAACAGG+AGG - tig0010967:56883-56902 Msa1349600:CDS 40.0%
TCCTCCTGTTATAGGATCAT+TGG + tig0010967:56887-56906 Msa1349620:intron 40.0%
AAAGATGAATCTGGCGAGAT+GGG + tig0010967:56967-56986 Msa1349620:intron 40.0%
AAAAGATGAATCTGGCGAGA+TGG + tig0010967:56968-56987 Msa1349620:intron 40.0%
TACCAAGTGAATGGATCACA+GGG + tig0010967:57211-57230 Msa1349620:intron 40.0%
GCTTGTGCTTCAGAAATCTA+AGG + tig0010967:57343-57362 Msa1349620:intron 40.0%
TGAAACTCTGGTTGGGAATT+TGG + tig0010967:55488-55507 None:intergenic 40.0%
AATGGCATGAAACTCTGGTT+GGG + tig0010967:55495-55514 None:intergenic 40.0%
TAATGGCATGAAACTCTGGT+TGG + tig0010967:55496-55515 None:intergenic 40.0%
CAGAGTTTCATGCCATTATC+CGG - tig0010967:55498-55517 Msa1349600:three_prime_UTR 40.0%
AGAGTTTCATGCCATTATCC+GGG - tig0010967:55499-55518 Msa1349600:three_prime_UTR 40.0%
!! GGGGCCTCTTTTTGATAATA+TGG + tig0010967:55674-55693 Msa1349610:CDS 40.0%
TCAAAAAGAGGCCCCAAATT+CGG - tig0010967:55679-55698 Msa1349600:three_prime_UTR 40.0%
AAGTGATCTACTATCTCTGC+TGG + tig0010967:55721-55740 Msa1349610:intron 40.0%
AGCTGTTTACTCGATGCAAT+CGG + tig0010967:55898-55917 Msa1349610:intron 40.0%
GGGAGATGATTTAACTGTTG+CGG - tig0010967:56025-56044 Msa1349600:three_prime_UTR 40.0%
TGGCATGGTAGAATGGAAAA+AGG - tig0010967:56153-56172 Msa1349600:three_prime_UTR 40.0%
GAACAAATCCCATTCACAAC+AGG - tig0010967:56175-56194 Msa1349600:three_prime_UTR 40.0%
!! CAAACCTGGAAGCATAGTTT+TGG - tig0010967:56352-56371 Msa1349600:three_prime_UTR 40.0%
GTTACCAAAACTATGCTTCC+AGG + tig0010967:56359-56378 Msa1349620:intron 40.0%
AGCTGAATGTGAGTCAAACA+TGG - tig0010967:56540-56559 Msa1349600:CDS 40.0%
GCAACCAATGATCCTATAAC+AGG - tig0010967:56880-56899 Msa1349600:CDS 40.0%
ACCAATGATCCTATAACAGG+AGG - tig0010967:56883-56902 Msa1349600:CDS 40.0%
TCCTCCTGTTATAGGATCAT+TGG + tig0010967:56887-56906 Msa1349620:intron 40.0%
AAAGATGAATCTGGCGAGAT+GGG + tig0010967:56967-56986 Msa1349620:intron 40.0%
AAAAGATGAATCTGGCGAGA+TGG + tig0010967:56968-56987 Msa1349620:intron 40.0%
TACCAAGTGAATGGATCACA+GGG + tig0010967:57211-57230 Msa1349620:intron 40.0%
GCTTGTGCTTCAGAAATCTA+AGG + tig0010967:57343-57362 Msa1349620:intron 40.0%
! CGGATAATGGCATGAAACTC+TGG + tig0010967:55500-55519 None:intergenic 45.0%
TTTGCTAGGCGAAAAGCTGA+GGG + tig0010967:55554-55573 None:intergenic 45.0%
! TTTTGCTAGGCGAAAAGCTG+AGG + tig0010967:55555-55574 None:intergenic 45.0%
CAAAAAGAGGCCCCAAATTC+GGG - tig0010967:55680-55699 Msa1349600:three_prime_UTR 45.0%
ATGCAATCGGGGTTACTTCT+AGG + tig0010967:55885-55904 Msa1349610:intron 45.0%
CTGTTTACTCGATGCAATCG+GGG + tig0010967:55896-55915 Msa1349610:intron 45.0%
GCTGTTTACTCGATGCAATC+GGG + tig0010967:55897-55916 Msa1349610:intron 45.0%
TTCTTACAGCAGCTTACTGC+TGG - tig0010967:55960-55979 Msa1349600:three_prime_UTR 45.0%
GACTCACATTCAGCTGCATT+TGG + tig0010967:56535-56554 Msa1349620:intron 45.0%
ACATACATGCATGTTCCTGC+AGG - tig0010967:56606-56625 Msa1349600:intron 45.0%
CATACATGCATGTTCCTGCA+GGG - tig0010967:56607-56626 Msa1349600:intron 45.0%
TCTCGTTGTTGGATACATGG+TGG + tig0010967:56858-56877 Msa1349620:intron 45.0%
ACCATGTATCCAACAACGAG+AGG - tig0010967:56857-56876 Msa1349600:CDS 45.0%
CCATGTATCCAACAACGAGA+GGG - tig0010967:56858-56877 Msa1349600:CDS 45.0%
GATCCTATAACAGGAGGAAG+TGG - tig0010967:56889-56908 Msa1349600:CDS 45.0%
AAGATGAATCTGGCGAGATG+GGG + tig0010967:56966-56985 Msa1349620:intron 45.0%
TACCCTGTGATCCATTCACT+TGG - tig0010967:57206-57225 Msa1349600:CDS 45.0%
CTACCAAGTGAATGGATCAC+AGG + tig0010967:57212-57231 Msa1349620:intron 45.0%
GATCCATTCACTTGGTAGAC+CGG - tig0010967:57214-57233 Msa1349600:CDS 45.0%
TACGTGTCTGTATCGTGTCT+TGG + tig0010967:57402-57421 Msa1349620:intron 45.0%
! CGGATAATGGCATGAAACTC+TGG + tig0010967:55500-55519 None:intergenic 45.0%
TTTGCTAGGCGAAAAGCTGA+GGG + tig0010967:55554-55573 None:intergenic 45.0%
! TTTTGCTAGGCGAAAAGCTG+AGG + tig0010967:55555-55574 None:intergenic 45.0%
CAAAAAGAGGCCCCAAATTC+GGG - tig0010967:55680-55699 Msa1349600:three_prime_UTR 45.0%
ATGCAATCGGGGTTACTTCT+AGG + tig0010967:55885-55904 Msa1349610:intron 45.0%
CTGTTTACTCGATGCAATCG+GGG + tig0010967:55896-55915 Msa1349610:intron 45.0%
GCTGTTTACTCGATGCAATC+GGG + tig0010967:55897-55916 Msa1349610:intron 45.0%
TTCTTACAGCAGCTTACTGC+TGG - tig0010967:55960-55979 Msa1349600:three_prime_UTR 45.0%
GACTCACATTCAGCTGCATT+TGG + tig0010967:56535-56554 Msa1349620:intron 45.0%
ACATACATGCATGTTCCTGC+AGG - tig0010967:56606-56625 Msa1349600:intron 45.0%
CATACATGCATGTTCCTGCA+GGG - tig0010967:56607-56626 Msa1349600:intron 45.0%
TCTCGTTGTTGGATACATGG+TGG + tig0010967:56858-56877 Msa1349620:intron 45.0%
ACCATGTATCCAACAACGAG+AGG - tig0010967:56857-56876 Msa1349600:CDS 45.0%
CCATGTATCCAACAACGAGA+GGG - tig0010967:56858-56877 Msa1349600:CDS 45.0%
GATCCTATAACAGGAGGAAG+TGG - tig0010967:56889-56908 Msa1349600:CDS 45.0%
AAGATGAATCTGGCGAGATG+GGG + tig0010967:56966-56985 Msa1349620:intron 45.0%
TACCCTGTGATCCATTCACT+TGG - tig0010967:57206-57225 Msa1349600:CDS 45.0%
CTACCAAGTGAATGGATCAC+AGG + tig0010967:57212-57231 Msa1349620:intron 45.0%
GATCCATTCACTTGGTAGAC+CGG - tig0010967:57214-57233 Msa1349600:CDS 45.0%
TACGTGTCTGTATCGTGTCT+TGG + tig0010967:57402-57421 Msa1349620:intron 45.0%
GTTGCGGCAAAATGGCACTT+AGG - tig0010967:56041-56060 Msa1349600:three_prime_UTR 50.0%
AGCTGCAGCCTGTTGTGAAT+GGG + tig0010967:56186-56205 None:intergenic 50.0%
AAGCTGCAGCCTGTTGTGAA+TGG + tig0010967:56187-56206 None:intergenic 50.0%
GGTCTTCTTAAGAACCCTGC+AGG + tig0010967:56624-56643 Msa1349620:intron 50.0%
CCCTCTCGTTGTTGGATACA+TGG + tig0010967:56861-56880 Msa1349620:intron 50.0%
ATTGGTTGCCCTCTCGTTGT+TGG + tig0010967:56869-56888 Msa1349620:intron 50.0%
CCACCACTTCCTCCTGTTAT+AGG + tig0010967:56895-56914 Msa1349620:intron 50.0%
CCTATAACAGGAGGAAGTGG+TGG - tig0010967:56892-56911 Msa1349600:CDS 50.0%
ATAACAGGAGGAAGTGGTGG+AGG - tig0010967:56895-56914 Msa1349600:CDS 50.0%
ACCCAATAGTCGCCTATAGC+CGG + tig0010967:56921-56940 Msa1349620:intron 50.0%
AAGCCGGTCTACCAAGTGAA+TGG + tig0010967:57220-57239 Msa1349620:intron 50.0%
GTTGCGGCAAAATGGCACTT+AGG - tig0010967:56041-56060 Msa1349600:three_prime_UTR 50.0%
AGCTGCAGCCTGTTGTGAAT+GGG + tig0010967:56186-56205 None:intergenic 50.0%
AAGCTGCAGCCTGTTGTGAA+TGG + tig0010967:56187-56206 None:intergenic 50.0%
GGTCTTCTTAAGAACCCTGC+AGG + tig0010967:56624-56643 Msa1349620:intron 50.0%
CCCTCTCGTTGTTGGATACA+TGG + tig0010967:56861-56880 Msa1349620:intron 50.0%
ATTGGTTGCCCTCTCGTTGT+TGG + tig0010967:56869-56888 Msa1349620:intron 50.0%
CCACCACTTCCTCCTGTTAT+AGG + tig0010967:56895-56914 Msa1349620:intron 50.0%
CCTATAACAGGAGGAAGTGG+TGG - tig0010967:56892-56911 Msa1349600:CDS 50.0%
ATAACAGGAGGAAGTGGTGG+AGG - tig0010967:56895-56914 Msa1349600:CDS 50.0%
ACCCAATAGTCGCCTATAGC+CGG + tig0010967:56921-56940 Msa1349620:intron 50.0%
AAGCCGGTCTACCAAGTGAA+TGG + tig0010967:57220-57239 Msa1349620:intron 50.0%
ACAGCAGCTTACTGCTGGCA+TGG - tig0010967:55965-55984 Msa1349600:three_prime_UTR 55.0%
CAGCAGCTTACTGCTGGCAT+GGG - tig0010967:55966-55985 Msa1349600:three_prime_UTR 55.0%
TGCTGTCCTCATCAGTGCTG+CGG - tig0010967:56830-56849 Msa1349600:intron 55.0%
GCCGGCTATAGGCGACTATT+GGG - tig0010967:56917-56936 Msa1349600:CDS 55.0%
ACAGCAGCTTACTGCTGGCA+TGG - tig0010967:55965-55984 Msa1349600:three_prime_UTR 55.0%
CAGCAGCTTACTGCTGGCAT+GGG - tig0010967:55966-55985 Msa1349600:three_prime_UTR 55.0%
TGCTGTCCTCATCAGTGCTG+CGG - tig0010967:56830-56849 Msa1349600:intron 55.0%
GCCGGCTATAGGCGACTATT+GGG - tig0010967:56917-56936 Msa1349600:CDS 55.0%
!! GTGGAACCGCAGCACTGATG+AGG + tig0010967:56839-56858 Msa1349620:intron 60.0%
! AAGTGGTGGAGGCCGGCTAT+AGG - tig0010967:56906-56925 Msa1349600:CDS 60.0%
GGCCGGCTATAGGCGACTAT+TGG - tig0010967:56916-56935 Msa1349600:CDS 60.0%
!! GTGGAACCGCAGCACTGATG+AGG + tig0010967:56839-56858 Msa1349620:intron 60.0%
! AAGTGGTGGAGGCCGGCTAT+AGG - tig0010967:56906-56925 Msa1349600:CDS 60.0%
GGCCGGCTATAGGCGACTAT+TGG - tig0010967:56916-56935 Msa1349600:CDS 60.0%
! CAGGAGGAAGTGGTGGAGGC+CGG - tig0010967:56899-56918 Msa1349600:CDS 65.0%
! CAGGAGGAAGTGGTGGAGGC+CGG - tig0010967:56899-56918 Msa1349600:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0010967 gene 55472 57440 55472 ID=Msa1349600;Name=Msa1349600
tig0010967 mRNA 55472 57440 55472 ID=Msa1349600-mRNA-1;Parent=Msa1349600;Name=Msa1349600-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|1|0.8|1|0.75|0.6|5|817|225
tig0010967 exon 55472 55707 55472 ID=Msa1349600-mRNA-1:exon:7058;Parent=Msa1349600-mRNA-1
tig0010967 exon 55904 56590 55904 ID=Msa1349600-mRNA-1:exon:7057;Parent=Msa1349600-mRNA-1
tig0010967 exon 56667 56748 56667 ID=Msa1349600-mRNA-1:exon:7056;Parent=Msa1349600-mRNA-1
tig0010967 exon 56850 56964 56850 ID=Msa1349600-mRNA-1:exon:7055;Parent=Msa1349600-mRNA-1
tig0010967 exon 57066 57440 57066 ID=Msa1349600-mRNA-1:exon:7054;Parent=Msa1349600-mRNA-1
tig0010967 CDS 57066 57440 57066 ID=Msa1349600-mRNA-1:cds;Parent=Msa1349600-mRNA-1
tig0010967 CDS 56850 56964 56850 ID=Msa1349600-mRNA-1:cds;Parent=Msa1349600-mRNA-1
tig0010967 CDS 56667 56748 56667 ID=Msa1349600-mRNA-1:cds;Parent=Msa1349600-mRNA-1
tig0010967 CDS 56485 56590 56485 ID=Msa1349600-mRNA-1:cds;Parent=Msa1349600-mRNA-1
tig0010967 three_prime_UTR 55904 56484 55904 ID=Msa1349600-mRNA-1:three_prime_utr;Parent=Msa1349600-mRNA-1
tig0010967 three_prime_UTR 55472 55707 55472 ID=Msa1349600-mRNA-1:three_prime_utr;Parent=Msa1349600-mRNA-1
Gene Sequence

>Msa1349600

ATGGATGTGGTAGCCAAATTCCCAACCAGAGTTTCATGCCATTATCCGGGAATAAACTCAAACATCAAAAAGAGACTCGCCCTCAGCTTTTCGCCTAGCAAAACATCATGCAACTTGCACAAAAAGATGGAGATACAACAAAGAAGCATTTGTTTCAAGAATGTCAAAAACAGAACTTGGGGTAACAATAGAATTTCAGCCATATTATCAAAAAGAGGCCCCAAATTCGGGAAAAATTCAAACTCTCCAGCAGAGATAGTAGATCACTTCTACACATGTATCAATGAAAAAGAGCTAAAACAATTGGATGAATACATATCACAAGATGCTTGCTTTTATGACTATACCTTCATCAATCCATTTCAAGGAAAGAAGGAAGTGATGCATTTCTTACAGCAGCTTACTGCTGGCATGGGCCAGAATGTGAAATTCATAGTCAGAAATATATGCGAGGGAGATGATTTAACTGTTGCGGCAAAATGGCACTTAGAATGGAAAAAGGAACAAATCCCATTCACAACAGGCTGCAGCTTCTTCCAATTAGAAAAAGTAGAAGAAAACATGACAATTAGGAGAGCTGAGATATTTATTGAATCACTAGTCAAACCTGGAAGCATAGTTTTGGTAACTCCTAAAACAGAACCTAACTCTTTCAAGTGTTGTAATTCTTATACGTGA
Protein sequence

>Msa1349600

MDVVAKFPTRVSCHYPGINSNIKKRLALSFSPSKTSCNLHKKMEIQQRSICFKNVKNRTWGNNRISAILSKRGPKFGKNSNSPAEIVDHFYTCINEKELKQLDEYISQDACFYDYTFINPFQGKKEVMHFLQQLTAGMGQNVKFIVRNICEGDDLTVAAKWHLEWKKEQIPFTTGCSFFQLEKVEENMTIRRAEIFIESLVKPGSIVLVTPKTEPNSFKCCNSYT*