AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1351030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1351030 MtrunA17_Chr3g0133211 95.304 362 17 0 1 362 1 362 0.0 645
Msa1351030 MtrunA17_Chr4g0041721 75.824 364 64 5 1 362 1 342 0.0 526
Msa1351030 MtrunA17_Chr5g0412471 51.111 360 148 11 1 354 1 338 4.70e-104 310
Msa1351030 MtrunA17_Chr5g0427191 48.588 354 166 8 3 352 4 345 1.52e-102 306
Msa1351030 MtrunA17_Chr8g0367661 40.850 306 157 6 65 352 62 361 9.00e-64 207
Msa1351030 MtrunA17_Chr8g0364521 42.241 232 131 2 62 291 59 289 1.50e-53 180
Msa1351030 MtrunA17_Chr1g0208231 36.364 297 174 7 62 352 72 359 7.39e-51 174
Msa1351030 MtrunA17_Chr5g0414271 37.024 289 156 4 9 295 12 276 2.36e-50 171
Msa1351030 MtrunA17_Chr4g0053381 37.786 262 154 4 95 352 80 336 1.35e-49 170
Msa1351030 MtrunA17_Chr3g0134261 37.165 261 156 5 96 353 84 339 4.10e-46 160
Msa1351030 MtrunA17_Chr5g0401271 39.196 199 113 3 95 290 101 294 4.13e-37 137
Msa1351030 MtrunA17_Chr2g0323361 32.069 290 172 8 74 353 28 302 1.85e-35 131
Msa1351030 MtrunA17_Chr2g0280941 35.268 224 130 6 99 319 43 254 6.92e-34 126
Msa1351030 MtrunA17_Chr3g0134781 34.440 241 128 6 8 246 1 213 4.14e-31 120
Msa1351030 MtrunA17_Chr4g0054011 29.915 234 144 7 97 319 68 292 2.57e-15 75.9
Msa1351030 MtrunA17_Chr3g0102851 31.765 170 102 5 92 255 56 217 3.06e-15 75.5
Msa1351030 MtrunA17_Chr3g0138431 29.121 182 116 4 74 251 225 397 5.10e-15 76.3
Msa1351030 MtrunA17_Chr1g0148681 28.492 179 123 3 73 251 167 340 1.11e-14 74.7
Msa1351030 MtrunA17_Chr3g0102861 30.000 170 105 5 92 255 46 207 2.14e-13 69.7
Msa1351030 MtrunA17_Chr3g0103471 30.286 175 107 6 92 258 46 213 2.40e-12 66.6
Msa1351030 MtrunA17_Chr8g0370671 30.000 170 107 4 91 257 203 363 2.69e-12 67.8
Msa1351030 MtrunA17_Chr1g0161581 28.155 206 120 8 51 252 329 510 3.50e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1351030 AT5G65420.1 50.545 275 118 6 62 332 42 302 3.36e-82 253
Msa1351030 AT5G65420.3 49.123 285 117 7 62 332 42 312 4.41e-81 250
Msa1351030 AT2G22490.1 45.338 311 159 5 26 329 25 331 6.24e-81 251
Msa1351030 AT2G22490.2 45.016 311 161 4 26 329 25 332 1.40e-80 251
Msa1351030 AT5G10440.1 54.545 231 102 1 59 289 31 258 2.28e-78 243
Msa1351030 AT5G65420.4 48.708 271 118 5 10 278 9 260 1.63e-76 237
Msa1351030 AT5G10440.2 56.983 179 77 0 59 237 31 209 1.62e-66 210
Msa1351030 AT5G65420.2 55.932 177 77 1 62 237 42 218 4.50e-62 199
Msa1351030 AT1G70210.1 44.444 243 127 3 57 295 45 283 5.61e-62 202
Msa1351030 AT3G50070.1 41.518 224 120 4 97 317 86 301 2.85e-46 162
Msa1351030 AT4G34160.1 39.604 202 119 2 97 296 87 287 3.24e-45 159
Msa1351030 AT5G67260.1 39.474 228 122 6 97 319 96 312 3.54e-43 154
Msa1351030 AT5G67260.2 39.207 227 124 5 97 319 96 312 7.91e-43 154
Msa1351030 AT4G37630.2 43.421 152 84 2 101 251 76 226 4.24e-32 121
Msa1351030 AT4G03270.1 31.338 284 168 11 74 352 35 296 3.15e-31 120
Msa1351030 AT4G37630.3 42.857 154 84 3 101 251 76 228 7.59e-31 118
Msa1351030 AT4G37630.1 42.857 154 84 3 101 251 76 228 2.96e-30 118
Msa1351030 AT4G03270.2 31.338 284 167 12 74 352 35 295 2.46e-29 115
Msa1351030 AT5G02110.1 26.383 235 159 6 64 294 45 269 3.80e-18 84.7
Msa1351030 AT1G20610.1 31.429 210 127 8 82 288 197 392 1.21e-15 78.2
Msa1351030 AT1G77390.1 29.609 179 119 4 74 251 190 362 9.92e-15 75.5
Msa1351030 AT5G02110.2 30.612 147 96 3 140 286 92 232 1.03e-14 74.3
Msa1351030 AT4G37490.1 29.143 175 114 4 77 251 179 343 1.48e-14 75.1
Msa1351030 AT3G11520.1 30.058 173 109 4 91 260 185 348 1.65e-14 74.7
Msa1351030 AT3G11520.2 30.058 173 109 4 91 260 185 348 2.62e-14 73.9
Msa1351030 AT1G76310.1 26.728 217 149 4 72 288 187 393 2.02e-13 71.6
Msa1351030 AT1G76310.2 28.889 180 118 4 72 251 187 356 5.77e-13 70.1

Find 110 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGCAGGTCTTGACTTCT+TGG 0.156748 tig0012074:-1496 Msa1351030:intron
AATTGAACTAAAAGGGTTAT+TGG 0.215800 tig0012074:-2982 Msa1351030:five_prime_UTR
CTCTCTCACTTCAGTGTCTA+TGG 0.221876 tig0012074:+3147 None:intergenic
CTTTATTTAGATTATAGATT+TGG 0.224239 tig0012074:+3031 None:intergenic
TCCTCAGCCTCTTGATTTGC+AGG 0.243856 tig0012074:-1956 Msa1351030:intron
TAGGTTGCAGTTTGGAGATT+TGG 0.254751 tig0012074:-2687 Msa1351030:CDS
GGCTGTAGCTTGCTTATCTT+TGG 0.277760 tig0012074:-2004 Msa1351030:CDS
GTTCGAGCACATTTCGGTTT+TGG 0.278825 tig0012074:-2211 Msa1351030:CDS
CATGACTCACCTTATAGTTT+TGG 0.280433 tig0012074:+607 None:intergenic
GAATCGATCCAAGTAATTAA+TGG 0.321350 tig0012074:+2168 None:intergenic
TATGGTTTGGTTCGAAGTTT+CGG 0.337739 tig0012074:+3165 None:intergenic
TAAAGGGGTGAGTGGGGTTG+AGG 0.340672 tig0012074:+3250 None:intergenic
CAAATCTATAATCTAAATAA+AGG 0.347585 tig0012074:-3030 Msa1351030:five_prime_UTR
GAAAGTAACCTTATGGAATA+TGG 0.352022 tig0012074:+761 None:intergenic
TTTATTTAGATTATAGATTT+GGG 0.357724 tig0012074:+3032 None:intergenic
TCACTTCAGTGTCTATGGTT+TGG 0.358014 tig0012074:+3152 None:intergenic
ACGAGGATTTGTGGCGTTCT+AGG 0.359940 tig0012074:-2838 Msa1351030:CDS
TTATCTATCCATTAATTACT+TGG 0.373505 tig0012074:-2176 Msa1351030:CDS
TTTAGGGTAAAGGGGTGAGT+GGG 0.385838 tig0012074:+3243 None:intergenic
TTTGTTTAATTGAACTAAAA+GGG 0.388432 tig0012074:-2989 Msa1351030:five_prime_UTR
GTAGTAGCCCAGATGCTAAA+AGG 0.388944 tig0012074:-844 Msa1351030:CDS
CAAAGAGTACCAAAACTATA+AGG 0.393869 tig0012074:-616 Msa1351030:three_prime_UTR
CACTGTTCAATGACATCTCT+TGG 0.397078 tig0012074:+1008 None:intergenic
TTTGCTACCATCAATTGCTT+GGG 0.406060 tig0012074:+1419 None:intergenic
AAACATTTAGGTACCTTTGA+TGG 0.406098 tig0012074:+1637 None:intergenic
TATTTGAATAGGTTGCAGTT+TGG 0.406265 tig0012074:-2695 Msa1351030:CDS
CTGGTGCTGATTATTTGAAT+AGG 0.407191 tig0012074:-2706 Msa1351030:CDS
CTCTGCAATGGCAACTCATC+AGG 0.411138 tig0012074:+2776 None:intergenic
ATTTGCTACCATCAATTGCT+TGG 0.417048 tig0012074:+1418 None:intergenic
CTGTGGCAACATGTGTTGTT+GGG 0.427417 tig0012074:-1446 Msa1351030:CDS
TTATAGGTTCGAGCACATTT+CGG 0.428580 tig0012074:-2217 Msa1351030:intron
GGAATATGGTGGTGGTGGTT+CGG 0.429440 tig0012074:-2870 Msa1351030:CDS
TCCTTGGTATTGCATGCTCT+TGG 0.432351 tig0012074:-556 Msa1351030:three_prime_UTR
ATTATTGAAGTGTGTTGAAA+AGG 0.434517 tig0012074:-1032 Msa1351030:CDS
TTTAGCTTCAAACACAAACT+TGG 0.447842 tig0012074:+1823 None:intergenic
AGCTAAAACCATTCAGAGAA+TGG 0.449900 tig0012074:-1807 Msa1351030:CDS
AACACACCCATTGGGCTTTG+AGG 0.450882 tig0012074:+941 None:intergenic
ATTGCAGAGTGAAGAGTGTT+TGG 0.451360 tig0012074:-2762 Msa1351030:CDS
CATCATCATTAATCTTAGAA+AGG 0.451623 tig0012074:+1714 None:intergenic
TAAAAGCTCCATTCTCTGAA+TGG 0.457326 tig0012074:+1799 None:intergenic
AACACCATCATAACGTTGTT+GGG 0.457610 tig0012074:+2799 None:intergenic
ATATGGTGGTGGTGGTTCGG+TGG 0.464818 tig0012074:-2867 Msa1351030:CDS
GACACCAATGCTGGTGGTGG+TGG 0.467695 tig0012074:-887 Msa1351030:CDS
AACAAGAGAAAGAGAGAGAG+AGG 0.470907 tig0012074:+3193 None:intergenic
TGGTGGTGGTGGTTCGGTGG+AGG 0.471956 tig0012074:-2864 Msa1351030:CDS
AAACATTCAATGAAGGAGAA+TGG 0.477187 tig0012074:+1737 None:intergenic
GATGGGGAATATGGTGGTGG+TGG 0.478397 tig0012074:-2875 Msa1351030:CDS
GGGGAAACCCAAGCAATTGA+TGG 0.479575 tig0012074:-1426 Msa1351030:CDS
AATGTATCCAACACACCCAT+TGG 0.480352 tig0012074:+932 None:intergenic
AGAGTGTTTGGTTCTGATGT+TGG 0.485214 tig0012074:-2750 Msa1351030:CDS
TAACAGTCAAAGAAAATTGG+AGG 0.485659 tig0012074:-486 Msa1351030:three_prime_UTR
TTAGGGTAAAGGGGTGAGTG+GGG 0.486613 tig0012074:+3244 None:intergenic
AATGCAAAATATTTAACAGC+TGG 0.493099 tig0012074:+514 None:intergenic
TGGAGGTGTACGAGGATTTG+TGG 0.493364 tig0012074:-2847 Msa1351030:CDS
GATGATGGGGAATATGGTGG+TGG 0.494099 tig0012074:-2878 Msa1351030:CDS
AGCTTGGACAATGCAATTGT+TGG 0.494353 tig0012074:-2025 Msa1351030:CDS
GAACACCATCATAACGTTGT+TGG 0.495114 tig0012074:+2798 None:intergenic
GCTGTGGCAACATGTGTTGT+TGG 0.496061 tig0012074:-1447 Msa1351030:CDS
TCTGATTCAATACATAGAAA+AGG 0.496974 tig0012074:-1385 Msa1351030:intron
ATTGGGCTTTGAGGCACACT+AGG 0.501893 tig0012074:+950 None:intergenic
AATTTCAGAAGGGAAGAGCT+TGG 0.509011 tig0012074:-2041 Msa1351030:intron
ATGTATCCAACACACCCATT+GGG 0.513076 tig0012074:+933 None:intergenic
ACAGCTGCTGCAATCTCTGA+TGG 0.518373 tig0012074:+1465 None:intergenic
CTACAAAAGCTCTGATCCAC+AGG 0.518487 tig0012074:+805 None:intergenic
TTTGATGATGGGGAATATGG+TGG 0.519402 tig0012074:-2881 Msa1351030:CDS
CTAGAAACACAATCAAAACT+TGG 0.521362 tig0012074:+2935 None:intergenic
GTCAAAGAAAATTGGAGGAG+GGG 0.526134 tig0012074:-481 Msa1351030:three_prime_UTR
AGAAAGGAAACATTCAATGA+AGG 0.530701 tig0012074:+1730 None:intergenic
AGAGAGAGAGGGTCTTATGA+AGG 0.531795 tig0012074:+3205 None:intergenic
GTGATAAAATGAATAGTCCT+TGG 0.532264 tig0012074:-572 Msa1351030:three_prime_UTR
TGAACCACCACCACCAGCAT+TGG 0.536808 tig0012074:+883 None:intergenic
AGTCATCGATTGGATTCAAA+AGG 0.537030 tig0012074:-2645 Msa1351030:intron
ACCTGCAAATCAAGAGGCTG+AGG 0.539718 tig0012074:+1955 None:intergenic
CTAGAAATGAAGTCATCGAT+TGG 0.540927 tig0012074:-2655 Msa1351030:CDS
TAGTACTCAGCACATTGAAG+TGG 0.542367 tig0012074:-1778 Msa1351030:CDS
CAGGGAATTCATACACAGAA+AGG 0.549830 tig0012074:+2146 None:intergenic
AGTGTGCCTCAAAGCCCAAT+GGG 0.552076 tig0012074:-947 Msa1351030:CDS
AAAGAGTGTCAGCAATGGCC+TGG 0.553578 tig0012074:-2725 Msa1351030:CDS
ATCAGAGATTGCAGCAGCTG+TGG 0.553736 tig0012074:-1463 Msa1351030:CDS
ATGACCCAACAACGTTATGA+TGG 0.554188 tig0012074:-2803 Msa1351030:CDS
TAGTGTGCCTCAAAGCCCAA+TGG 0.559807 tig0012074:-948 Msa1351030:CDS
AGTCAAAGAAAATTGGAGGA+GGG 0.559877 tig0012074:-482 Msa1351030:three_prime_UTR
CAGTCAAAGAAAATTGGAGG+AGG 0.561515 tig0012074:-483 Msa1351030:three_prime_UTR
TATCTTCTTCAGTGCAAAGA+AGG 0.566069 tig0012074:+2912 None:intergenic
GTGAGTCATGAGTGGAAGGT+GGG 0.576350 tig0012074:-594 Msa1351030:three_prime_UTR
TCAAAGCCCAATGGGTGTGT+TGG 0.579780 tig0012074:-939 Msa1351030:CDS
CTTATATCAAGCACCATCAA+AGG 0.583083 tig0012074:-1650 Msa1351030:intron
TCATTGAACAGTGTGTTCAC+TGG 0.586627 tig0012074:-998 Msa1351030:CDS
GGTGAGTCATGAGTGGAAGG+TGG 0.594616 tig0012074:-595 Msa1351030:three_prime_UTR
CACTGAAGTGAGAGAGTGAG+TGG 0.594748 tig0012074:-3140 Msa1351030:five_prime_UTR
AAACACTCTTCACTCTGCAA+TGG 0.596088 tig0012074:+2764 None:intergenic
GAACAGTGTGTTCACTGGCA+AGG 0.596096 tig0012074:-993 Msa1351030:CDS
TTGGGCTTTGAGGCACACTA+GGG 0.596531 tig0012074:+951 None:intergenic
TAAGGTGAGTCATGAGTGGA+AGG 0.597213 tig0012074:-598 Msa1351030:three_prime_UTR
ATCTTTGGCAGCTAAAGTAG+AGG 0.600255 tig0012074:-1989 Msa1351030:CDS
AATCCTACCTGCAAATCAAG+AGG 0.610223 tig0012074:+1949 None:intergenic
ACTATAAGGTGAGTCATGAG+TGG 0.611274 tig0012074:-602 Msa1351030:three_prime_UTR
CTATTCAAATAATCAGCACC+AGG 0.612948 tig0012074:+2707 None:intergenic
ATGATGATAAGAGTTCATCA+AGG 0.613251 tig0012074:-1697 Msa1351030:CDS
TGGTTCGGTGGAGGTGTACG+AGG 0.618407 tig0012074:-2855 Msa1351030:CDS
ACAAGAGAAAGAGAGAGAGA+GGG 0.634472 tig0012074:+3194 None:intergenic
GATGACACCAATGCTGGTGG+TGG 0.635406 tig0012074:-890 Msa1351030:CDS
AAAAGTGATGACACCAATGC+TGG 0.640482 tig0012074:-896 Msa1351030:CDS
ATGGATAGATAAGCACAAAG+AGG 0.659013 tig0012074:+2187 None:intergenic
GCCAAGAGCATGCAATACCA+AGG 0.661537 tig0012074:+555 None:intergenic
TGTGGCAACATGTGTTGTTG+GGG 0.663318 tig0012074:-1445 Msa1351030:CDS
AGGAAGCTAAACAAAACCTG+TGG 0.680338 tig0012074:-821 Msa1351030:CDS
AGAGATGAGTATGTACAATG+TGG 0.690153 tig0012074:+3277 None:intergenic
GTAGCCCAGATGCTAAAAGG+AGG 0.696973 tig0012074:-841 Msa1351030:CDS
AGTGATGACACCAATGCTGG+TGG 0.729525 tig0012074:-893 Msa1351030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTTAGATTATAGATTT+GGG + tig0012074:641-660 None:intergenic 10.0%
!!! CTTTATTTAGATTATAGATT+TGG + tig0012074:642-661 None:intergenic 15.0%
!! CAAATCTATAATCTAAATAA+AGG - tig0012074:640-659 Msa1351030:three_prime_UTR 15.0%
!!! TTTTGTTTAATTGAACTAAA+AGG - tig0012074:680-699 Msa1351030:three_prime_UTR 15.0%
!! TTTGTTTAATTGAACTAAAA+GGG - tig0012074:681-700 Msa1351030:three_prime_UTR 15.0%
!!! TTTTTATGAAATCTTTTTGA+TGG - tig0012074:1108-1127 Msa1351030:intron 15.0%
!!! TTTTATGAAATCTTTTTGAT+GGG - tig0012074:1109-1128 Msa1351030:intron 15.0%
!!! TTTAAGTTGATTAATTTTGA+TGG - tig0012074:2143-2162 Msa1351030:intron 15.0%
!!! TATTGGATTTTCTGTTATTT+TGG - tig0012074:705-724 Msa1351030:three_prime_UTR 20.0%
!!! TTAATTTTTCATCAAAACAG+TGG - tig0012074:1157-1176 Msa1351030:intron 20.0%
!!! CAATTTTCTTTGTGATTTAT+AGG - tig0012074:1437-1456 Msa1351030:CDS 20.0%
!! TTATCTATCCATTAATTACT+TGG - tig0012074:1494-1513 Msa1351030:intron 20.0%
!! AATACATAGAAAAGGTAAAA+TGG - tig0012074:2293-2312 Msa1351030:intron 20.0%
!! TATTAACAATGTGTTAATTG+TGG - tig0012074:2601-2620 Msa1351030:intron 20.0%
!!! TTTTAACAGTCAAAGAAAAT+TGG - tig0012074:3181-3200 Msa1351030:five_prime_UTR 20.0%
! AATTGAACTAAAAGGGTTAT+TGG - tig0012074:688-707 Msa1351030:three_prime_UTR 25.0%
!!! GATTTTCTGTTATTTTGGAA+TGG - tig0012074:710-729 Msa1351030:three_prime_UTR 25.0%
!! ATATTGATTGTGAAAACTGA+AGG - tig0012074:1071-1090 Msa1351030:intron 25.0%
! TTTAATGAACTATCCCTTTA+TGG - tig0012074:1210-1229 Msa1351030:intron 25.0%
! TTGTGATATGTATCCATAAA+GGG + tig0012074:1226-1245 None:intergenic 25.0%
! ATTGTGATATGTATCCATAA+AGG + tig0012074:1227-1246 None:intergenic 25.0%
! TCGTTATTCATTAACTTACA+GGG + tig0012074:1545-1564 None:intergenic 25.0%
! GAAGTTCAACACAAAAAAAT+AGG - tig0012074:1565-1584 Msa1351030:intron 25.0%
! TTATGTTTCCAATTTCAGAA+GGG - tig0012074:1619-1638 Msa1351030:intron 25.0%
! CATCATCATTAATCTTAGAA+AGG + tig0012074:1959-1978 None:intergenic 25.0%
! AGTGAAAACAATCATGAATT+AGG - tig0012074:2116-2135 Msa1351030:intron 25.0%
! TCTGATTCAATACATAGAAA+AGG - tig0012074:2285-2304 Msa1351030:intron 25.0%
!! TACATTGTTTTCTTGTCATT+TGG + tig0012074:2328-2347 None:intergenic 25.0%
! CTCATATAACAACTTAAACA+AGG - tig0012074:2478-2497 Msa1351030:intron 25.0%
! ATTATTGAAGTGTGTTGAAA+AGG - tig0012074:2638-2657 Msa1351030:intron 25.0%
!! GAATTTTACCATATTCCATA+AGG - tig0012074:2901-2920 Msa1351030:CDS 25.0%
!!! TTTTTTTGGTATTGAACTGA+GGG + tig0012074:2974-2993 None:intergenic 25.0%
! AATGCAAAATATTTAACAGC+TGG + tig0012074:3159-3178 None:intergenic 25.0%
! TTTTAATGATGTTGTGTGTG+TGG + tig0012074:556-575 None:intergenic 30.0%
CTAGAAACACAATCAAAACT+TGG + tig0012074:738-757 None:intergenic 30.0%
!! ATAGCAGTGTTTTTGATGAT+GGG - tig0012074:778-797 Msa1351030:three_prime_UTR 30.0%
TATTTGAATAGGTTGCAGTT+TGG - tig0012074:975-994 Msa1351030:CDS 30.0%
! ATTTTTCATCAAAACAGTGG+CGG - tig0012074:1160-1179 Msa1351030:intron 30.0%
! ATCCATTGCTTGATAACTAT+TGG - tig0012074:1264-1283 Msa1351030:intron 30.0%
TCATCAATTCATCAGATACA+AGG + tig0012074:1295-1314 None:intergenic 30.0%
! TGATGAATTGATGACTTGAA+TGG - tig0012074:1301-1320 Msa1351030:intron 30.0%
TAGCACATTTGATTGATGAA+TGG - tig0012074:1334-1353 Msa1351030:intron 30.0%
!! ATTGATGAATGGATTGAGAT+TGG - tig0012074:1345-1364 Msa1351030:intron 30.0%
GAATCGATCCAAGTAATTAA+TGG + tig0012074:1505-1524 None:intergenic 30.0%
CTCGTTATTCATTAACTTAC+AGG + tig0012074:1546-1565 None:intergenic 30.0%
! ATAGGCTTTATATTCTGCAT+TGG - tig0012074:1583-1602 Msa1351030:intron 30.0%
GTTATGTTTCCAATTTCAGA+AGG - tig0012074:1618-1637 Msa1351030:intron 30.0%
AACTTGTCAATTTCAATGCT+TGG - tig0012074:1747-1766 Msa1351030:CDS 30.0%
! TTTAGCTTCAAACACAAACT+TGG + tig0012074:1850-1869 None:intergenic 30.0%
AAACATTCAATGAAGGAGAA+TGG + tig0012074:1936-1955 None:intergenic 30.0%
AGAAAGGAAACATTCAATGA+AGG + tig0012074:1943-1962 None:intergenic 30.0%
ATGATGATAAGAGTTCATCA+AGG - tig0012074:1973-1992 Msa1351030:CDS 30.0%
AAACATTTAGGTACCTTTGA+TGG + tig0012074:2036-2055 None:intergenic 30.0%
ACATAGAAAAGGTAAAATGG+TGG - tig0012074:2296-2315 Msa1351030:intron 30.0%
!! TGTTTTCTTGTCATTTGGAA+AGG + tig0012074:2323-2342 None:intergenic 30.0%
TTAGTTCATACATACTCTCA+AGG - tig0012074:2366-2385 Msa1351030:intron 30.0%
GTGTTAATTGTGGTTATTAC+AGG - tig0012074:2611-2630 Msa1351030:intron 30.0%
GAAAGTAACCTTATGGAATA+TGG + tig0012074:2912-2931 None:intergenic 30.0%
!!! ACACATAATGTCCTTTTTTG+GGG + tig0012074:2934-2953 None:intergenic 30.0%
!!! CACACATAATGTCCTTTTTT+GGG + tig0012074:2935-2954 None:intergenic 30.0%
!!! ACACACATAATGTCCTTTTT+TGG + tig0012074:2936-2955 None:intergenic 30.0%
! AGGACATTATGTGTGTTTTA+TGG - tig0012074:2940-2959 Msa1351030:CDS 30.0%
! CATTATGTGTGTTTTATGGA+AGG - tig0012074:2944-2963 Msa1351030:CDS 30.0%
!!! TTTTTTGGTATTGAACTGAG+GGG + tig0012074:2973-2992 None:intergenic 30.0%
!!! CTTTTTTTGGTATTGAACTG+AGG + tig0012074:2975-2994 None:intergenic 30.0%
CTCAGTTCAATACCAAAAAA+AGG - tig0012074:2973-2992 Msa1351030:five_prime_UTR 30.0%
!!! GCTATATCTCTTCCTTTTTT+TGG + tig0012074:2988-3007 None:intergenic 30.0%
!! CAAAGAGTACCAAAACTATA+AGG - tig0012074:3054-3073 Msa1351030:five_prime_UTR 30.0%
GTGATAAAATGAATAGTCCT+TGG - tig0012074:3098-3117 Msa1351030:five_prime_UTR 30.0%
TAACAGTCAAAGAAAATTGG+AGG - tig0012074:3184-3203 Msa1351030:five_prime_UTR 30.0%
AGAGATGAGTATGTACAATG+TGG + tig0012074:396-415 None:intergenic 35.0%
!! TATGGTTTGGTTCGAAGTTT+CGG + tig0012074:508-527 None:intergenic 35.0%
TATCTTCTTCAGTGCAAAGA+AGG + tig0012074:761-780 None:intergenic 35.0%
!! GATAGCAGTGTTTTTGATGA+TGG - tig0012074:777-796 Msa1351030:three_prime_UTR 35.0%
!!! TAGCAGTGTTTTTGATGATG+GGG - tig0012074:779-798 Msa1351030:three_prime_UTR 35.0%
!!! GTTTTTGATGATGGGGAATA+TGG - tig0012074:786-805 Msa1351030:exon 35.0%
AACACCATCATAACGTTGTT+GGG + tig0012074:874-893 None:intergenic 35.0%
CTATTCAAATAATCAGCACC+AGG + tig0012074:966-985 None:intergenic 35.0%
!! CTGGTGCTGATTATTTGAAT+AGG - tig0012074:964-983 Msa1351030:CDS 35.0%
! GTTTGGAGATTTGGATTTTC+AGG - tig0012074:992-1011 Msa1351030:CDS 35.0%
CTAGAAATGAAGTCATCGAT+TGG - tig0012074:1015-1034 Msa1351030:CDS 35.0%
! AGTCATCGATTGGATTCAAA+AGG - tig0012074:1025-1044 Msa1351030:CDS 35.0%
!!! CGTTTTAATTGGGTTTGGAT+TGG - tig0012074:1186-1205 Msa1351030:intron 35.0%
GTCCAATAGTTATCAAGCAA+TGG + tig0012074:1269-1288 None:intergenic 35.0%
! GGATTGAGATTGGATTAGAT+TGG - tig0012074:1355-1374 Msa1351030:intron 35.0%
! GAGATTGGATTAGATTGGAT+TGG - tig0012074:1360-1379 Msa1351030:intron 35.0%
TTATAGGTTCGAGCACATTT+CGG - tig0012074:1453-1472 Msa1351030:CDS 35.0%
ATGGATAGATAAGCACAAAG+AGG + tig0012074:1486-1505 None:intergenic 35.0%
AGCTAAAACCATTCAGAGAA+TGG - tig0012074:1863-1882 Msa1351030:intron 35.0%
TAAAAGCTCCATTCTCTGAA+TGG + tig0012074:1874-1893 None:intergenic 35.0%
CTTATATCAAGCACCATCAA+AGG - tig0012074:2020-2039 Msa1351030:CDS 35.0%
TCACTTGAGCAATGAAGTTA+GGG + tig0012074:2063-2082 None:intergenic 35.0%
TTCACTTGAGCAATGAAGTT+AGG + tig0012074:2064-2083 None:intergenic 35.0%
! ATGATTGTTTTCACTGCAAG+GGG + tig0012074:2111-2130 None:intergenic 35.0%
! CATGATTGTTTTCACTGCAA+GGG + tig0012074:2112-2131 None:intergenic 35.0%
! TCATGATTGTTTTCACTGCA+AGG + tig0012074:2113-2132 None:intergenic 35.0%
TTTGCTACCATCAATTGCTT+GGG + tig0012074:2254-2273 None:intergenic 35.0%
! ATTTGCTACCATCAATTGCT+TGG + tig0012074:2255-2274 None:intergenic 35.0%
!! TTTTGGGGAAAGTAACCTTA+TGG + tig0012074:2919-2938 None:intergenic 35.0%
! AAGGTTACTTTCCCCAAAAA+AGG - tig0012074:2920-2939 Msa1351030:CDS 35.0%
! CATGACTCACCTTATAGTTT+TGG + tig0012074:3066-3085 None:intergenic 35.0%
AGTCAAAGAAAATTGGAGGA+GGG - tig0012074:3188-3207 Msa1351030:five_prime_UTR 35.0%
!! GTAGAGTCTGAGATTTTCTT+TGG - tig0012074:3242-3261 Msa1351030:five_prime_UTR 35.0%
!!! ACGCCTCTTTTTAGGGTAAA+GGG + tig0012074:439-458 None:intergenic 40.0%
!!! AACGCCTCTTTTTAGGGTAA+AGG + tig0012074:440-459 None:intergenic 40.0%
!! TTACCCTAAAAAGAGGCGTT+CGG - tig0012074:440-459 Msa1351030:three_prime_UTR 40.0%
ACAAGAGAAAGAGAGAGAGA+GGG + tig0012074:479-498 None:intergenic 40.0%
AACAAGAGAAAGAGAGAGAG+AGG + tig0012074:480-499 None:intergenic 40.0%
TCACTTCAGTGTCTATGGTT+TGG + tig0012074:521-540 None:intergenic 40.0%
!! TTTGATGATGGGGAATATGG+TGG - tig0012074:789-808 Msa1351030:exon 40.0%
ATGACCCAACAACGTTATGA+TGG - tig0012074:867-886 Msa1351030:CDS 40.0%
GAACACCATCATAACGTTGT+TGG + tig0012074:875-894 None:intergenic 40.0%
AAACACTCTTCACTCTGCAA+TGG + tig0012074:909-928 None:intergenic 40.0%
!! ATTGCAGAGTGAAGAGTGTT+TGG - tig0012074:908-927 Msa1351030:CDS 40.0%
!! AGAGTGTTTGGTTCTGATGT+TGG - tig0012074:920-939 Msa1351030:CDS 40.0%
!! TGGAAAAAGAGTGTCAGCAA+TGG - tig0012074:940-959 Msa1351030:CDS 40.0%
TAGGTTGCAGTTTGGAGATT+TGG - tig0012074:983-1002 Msa1351030:CDS 40.0%
CAGGGAATTCATACACAGAA+AGG + tig0012074:1527-1546 None:intergenic 40.0%
!! AAGCTCTTCCCTTCTGAAAT+TGG + tig0012074:1630-1649 None:intergenic 40.0%
AATTTCAGAAGGGAAGAGCT+TGG - tig0012074:1629-1648 Msa1351030:intron 40.0%
AGCTTGGACAATGCAATTGT+TGG - tig0012074:1645-1664 Msa1351030:intron 40.0%
ATCTTTGGCAGCTAAAGTAG+AGG - tig0012074:1681-1700 Msa1351030:CDS 40.0%
AATCCTACCTGCAAATCAAG+AGG + tig0012074:1724-1743 None:intergenic 40.0%
!! CTTGATTTGCAGGTAGGATT+TGG - tig0012074:1724-1743 Msa1351030:CDS 40.0%
!! GTCGATTTTTGTGTGCAGAT+TGG - tig0012074:1819-1838 Msa1351030:CDS 40.0%
TAGTACTCAGCACATTGAAG+TGG - tig0012074:1892-1911 Msa1351030:intron 40.0%
AGTTAGGGAGGGAAACATTT+AGG + tig0012074:2048-2067 None:intergenic 40.0%
TTGAGCAATGAAGTTAGGGA+GGG + tig0012074:2059-2078 None:intergenic 40.0%
! TGATGGTGTGAATTCAATGC+AGG - tig0012074:2160-2179 Msa1351030:CDS 40.0%
CACTGTTCAATGACATCTCT+TGG + tig0012074:2665-2684 None:intergenic 40.0%
TCATTGAACAGTGTGTTCAC+TGG - tig0012074:2672-2691 Msa1351030:CDS 40.0%
ATGTATCCAACACACCCATT+GGG + tig0012074:2740-2759 None:intergenic 40.0%
AATGTATCCAACACACCCAT+TGG + tig0012074:2741-2760 None:intergenic 40.0%
AAAAGTGATGACACCAATGC+TGG - tig0012074:2774-2793 Msa1351030:CDS 40.0%
AGGAAGCTAAACAAAACCTG+TGG - tig0012074:2849-2868 Msa1351030:CDS 40.0%
! TCAGAGCTTTTGTAGTCATG+TGG - tig0012074:2873-2892 Msa1351030:CDS 40.0%
! CTTTTGTAGTCATGTGGCAT+GGG - tig0012074:2879-2898 Msa1351030:CDS 40.0%
ACTATAAGGTGAGTCATGAG+TGG - tig0012074:3068-3087 Msa1351030:five_prime_UTR 40.0%
CAGTCAAAGAAAATTGGAGG+AGG - tig0012074:3187-3206 Msa1351030:five_prime_UTR 40.0%
GTCAAAGAAAATTGGAGGAG+GGG - tig0012074:3189-3208 Msa1351030:five_prime_UTR 40.0%
! TTTAGGGTAAAGGGGTGAGT+GGG + tig0012074:430-449 None:intergenic 45.0%
!!! TTTTAGGGTAAAGGGGTGAG+TGG + tig0012074:431-450 None:intergenic 45.0%
CACCCCTTTACCCTAAAAAG+AGG - tig0012074:433-452 Msa1351030:three_prime_UTR 45.0%
!!! CGCCTCTTTTTAGGGTAAAG+GGG + tig0012074:438-457 None:intergenic 45.0%
! GAACCGAACGCCTCTTTTTA+GGG + tig0012074:446-465 None:intergenic 45.0%
! AGAGAGAGAGGGTCTTATGA+AGG + tig0012074:468-487 None:intergenic 45.0%
CTCTCTCACTTCAGTGTCTA+TGG + tig0012074:526-545 None:intergenic 45.0%
TTTCATCAAAACAGTGGCGG+CGG - tig0012074:1163-1182 Msa1351030:intron 45.0%
! GTTCGAGCACATTTCGGTTT+TGG - tig0012074:1459-1478 Msa1351030:CDS 45.0%
!! GGCTGTAGCTTGCTTATCTT+TGG - tig0012074:1666-1685 Msa1351030:CDS 45.0%
CTTGAGCAATGAAGTTAGGG+AGG + tig0012074:2060-2079 None:intergenic 45.0%
! TTGTTTTCACTGCAAGGGGT+TGG + tig0012074:2107-2126 None:intergenic 45.0%
CAATGCAGGTCTTGACTTCT+TGG - tig0012074:2174-2193 Msa1351030:CDS 45.0%
! CTGTGGCAACATGTGTTGTT+GGG - tig0012074:2224-2243 Msa1351030:intron 45.0%
! TGTGGCAACATGTGTTGTTG+GGG - tig0012074:2225-2244 Msa1351030:intron 45.0%
GTAGTAGCCCAGATGCTAAA+AGG - tig0012074:2826-2845 Msa1351030:CDS 45.0%
! GCTTCCTCCTTTTAGCATCT+GGG + tig0012074:2836-2855 None:intergenic 45.0%
! AGCTTCCTCCTTTTAGCATC+TGG + tig0012074:2837-2856 None:intergenic 45.0%
! CTACAAAAGCTCTGATCCAC+AGG + tig0012074:2868-2887 None:intergenic 45.0%
! GCTTTTGTAGTCATGTGGCA+TGG - tig0012074:2878-2897 Msa1351030:CDS 45.0%
TAAGGTGAGTCATGAGTGGA+AGG - tig0012074:3072-3091 Msa1351030:five_prime_UTR 45.0%
TCCTTGGTATTGCATGCTCT+TGG - tig0012074:3114-3133 Msa1351030:five_prime_UTR 45.0%
TTAGGGTAAAGGGGTGAGTG+GGG + tig0012074:429-448 None:intergenic 50.0%
! GGAACCGAACGCCTCTTTTT+AGG + tig0012074:447-466 None:intergenic 50.0%
CACTGAAGTGAGAGAGTGAG+TGG - tig0012074:530-549 Msa1351030:three_prime_UTR 50.0%
!! GATGATGGGGAATATGGTGG+TGG - tig0012074:792-811 Msa1351030:exon 50.0%
!! GGAATATGGTGGTGGTGGTT+CGG - tig0012074:800-819 Msa1351030:exon 50.0%
! TGGAGGTGTACGAGGATTTG+TGG - tig0012074:823-842 Msa1351030:CDS 50.0%
ACGAGGATTTGTGGCGTTCT+AGG - tig0012074:832-851 Msa1351030:CDS 50.0%
CTCTGCAATGGCAACTCATC+AGG + tig0012074:897-916 None:intergenic 50.0%
!! AAAGAGTGTCAGCAATGGCC+TGG - tig0012074:945-964 Msa1351030:CDS 50.0%
!!! GGCGGCGTTTTAATTGGGTT+TGG - tig0012074:1181-1200 Msa1351030:intron 50.0%
!! TCCTCAGCCTCTTGATTTGC+AGG - tig0012074:1714-1733 Msa1351030:CDS 50.0%
ACCTGCAAATCAAGAGGCTG+AGG + tig0012074:1718-1737 None:intergenic 50.0%
!! CAGCCTCTTGATTTGCAGGT+AGG - tig0012074:1718-1737 Msa1351030:CDS 50.0%
ACAGCTGCTGCAATCTCTGA+TGG + tig0012074:2208-2227 None:intergenic 50.0%
ATCAGAGATTGCAGCAGCTG+TGG - tig0012074:2207-2226 Msa1351030:CDS 50.0%
GCTGTGGCAACATGTGTTGT+TGG - tig0012074:2223-2242 Msa1351030:intron 50.0%
GGGGAAACCCAAGCAATTGA+TGG - tig0012074:2244-2263 Msa1351030:intron 50.0%
GAACAGTGTGTTCACTGGCA+AGG - tig0012074:2677-2696 Msa1351030:CDS 50.0%
!! TTGGGCTTTGAGGCACACTA+GGG + tig0012074:2722-2741 None:intergenic 50.0%
!! ATTGGGCTTTGAGGCACACT+AGG + tig0012074:2723-2742 None:intergenic 50.0%
TAGTGTGCCTCAAAGCCCAA+TGG - tig0012074:2722-2741 Msa1351030:CDS 50.0%
AGTGTGCCTCAAAGCCCAAT+GGG - tig0012074:2723-2742 Msa1351030:CDS 50.0%
AACACACCCATTGGGCTTTG+AGG + tig0012074:2732-2751 None:intergenic 50.0%
TCAAAGCCCAATGGGTGTGT+TGG - tig0012074:2731-2750 Msa1351030:CDS 50.0%
AGTGATGACACCAATGCTGG+TGG - tig0012074:2777-2796 Msa1351030:CDS 50.0%
GTAGCCCAGATGCTAAAAGG+AGG - tig0012074:2829-2848 Msa1351030:CDS 50.0%
GTGAGTCATGAGTGGAAGGT+GGG - tig0012074:3076-3095 Msa1351030:five_prime_UTR 50.0%
! GCCAAGAGCATGCAATACCA+AGG + tig0012074:3118-3137 None:intergenic 50.0%
TAAAGGGGTGAGTGGGGTTG+AGG + tig0012074:423-442 None:intergenic 55.0%
!! GATGGGGAATATGGTGGTGG+TGG - tig0012074:795-814 Msa1351030:exon 55.0%
!! ATATGGTGGTGGTGGTTCGG+TGG - tig0012074:803-822 Msa1351030:exon 55.0%
!! AGTGGCGGCGGCGTTTTAAT+TGG - tig0012074:1175-1194 Msa1351030:intron 55.0%
!!! GTGGCGGCGGCGTTTTAATT+GGG - tig0012074:1176-1195 Msa1351030:intron 55.0%
GATGACACCAATGCTGGTGG+TGG - tig0012074:2780-2799 Msa1351030:CDS 55.0%
TGAACCACCACCACCAGCAT+TGG + tig0012074:2790-2809 None:intergenic 55.0%
GGTGAGTCATGAGTGGAAGG+TGG - tig0012074:3075-3094 Msa1351030:five_prime_UTR 55.0%
!! TGGTTCGGTGGAGGTGTACG+AGG - tig0012074:815-834 Msa1351030:CDS 60.0%
!! GACACCAATGCTGGTGGTGG+TGG - tig0012074:2783-2802 Msa1351030:CDS 60.0%
!! TGGTGGTGGTGGTTCGGTGG+AGG - tig0012074:806-825 Msa1351030:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0012074 gene 387 3305 387 ID=Msa1351030;Name=Msa1351030
tig0012074 mRNA 387 3305 387 ID=Msa1351030-mRNA-1;Parent=Msa1351030;Name=Msa1351030-mRNA-1;_AED=0.04;_eAED=0.04;_QI=342|1|1|1|1|1|6|418|362
tig0012074 exon 387 1059 387 ID=Msa1351030-mRNA-1:exon:7636;Parent=Msa1351030-mRNA-1
tig0012074 exon 1386 1510 1386 ID=Msa1351030-mRNA-1:exon:7635;Parent=Msa1351030-mRNA-1
tig0012074 exon 1651 1855 1651 ID=Msa1351030-mRNA-1:exon:7634;Parent=Msa1351030-mRNA-1
tig0012074 exon 1957 2055 1957 ID=Msa1351030-mRNA-1:exon:7633;Parent=Msa1351030-mRNA-1
tig0012074 exon 2147 2233 2147 ID=Msa1351030-mRNA-1:exon:7632;Parent=Msa1351030-mRNA-1
tig0012074 exon 2646 3305 2646 ID=Msa1351030-mRNA-1:exon:7631;Parent=Msa1351030-mRNA-1
tig0012074 five_prime_UTR 2964 3305 2964 ID=Msa1351030-mRNA-1:five_prime_utr;Parent=Msa1351030-mRNA-1
tig0012074 CDS 2646 2963 2646 ID=Msa1351030-mRNA-1:cds;Parent=Msa1351030-mRNA-1
tig0012074 CDS 2147 2233 2147 ID=Msa1351030-mRNA-1:cds;Parent=Msa1351030-mRNA-1
tig0012074 CDS 1957 2055 1957 ID=Msa1351030-mRNA-1:cds;Parent=Msa1351030-mRNA-1
tig0012074 CDS 1651 1855 1651 ID=Msa1351030-mRNA-1:cds;Parent=Msa1351030-mRNA-1
tig0012074 CDS 1386 1510 1386 ID=Msa1351030-mRNA-1:cds;Parent=Msa1351030-mRNA-1
tig0012074 CDS 805 1059 805 ID=Msa1351030-mRNA-1:cds;Parent=Msa1351030-mRNA-1
tig0012074 three_prime_UTR 387 804 387 ID=Msa1351030-mRNA-1:three_prime_utr;Parent=Msa1351030-mRNA-1
Gene Sequence

>Msa1351030

ATGGCACCAAGTTTTGATTGTGTTTCTAGCCTTCTTTGCACTGAAGAAGATAGCAGTGTTTTTGATGATGGGGAATATGGTGGTGGTGGTTCGGTGGAGGTGTACGAGGATTTGTGGCGTTCTAGGAATGATCATCAGATGACCCAACAACGTTATGATGGTGTTCCTGATGAGTTGCCATTGCAGAGTGAAGAGTGTTTGGTTCTGATGTTGGAAAAAGAGTGTCAGCAATGGCCTGGTGCTGATTATTTGAATAGGTTGCAGTTTGGAGATTTGGATTTTCAGGCTAGAAATGAAGTCATCGATTGGATTCAAAAGGTTCGAGCACATTTCGGTTTTGGTCCTCTTTGTGCTTATCTATCCATTAATTACTTGGATCGATTCCTTTCTGTGTATGAATTCCCTAAGGGAAGAGCTTGGACAATGCAATTGTTGGCTGTAGCTTGCTTATCTTTGGCAGCTAAAGTAGAGGAAACTGCTGTTCCTCAGCCTCTTGATTTGCAGATTGGTGAATCCAAGTTTGTGTTTGAAGCTAAAACCATTCAGAGAATGGAGCTTTTAGTACTCAGCACATTGAAGTGGAGAATGCAAGCAATCACTCCATTCTCCTTCATTGAATGTTTCCTTTCTAAGATTAATGATGATGATAAGAGTTCATCAAGGTTTTCAATTTTGCGATCAACTCAACTTATATCAAGCACCATCAAAGGTCTTGACTTCTTGGAATTCAAACCATCAGAGATTGCAGCAGCTGTGGCAACATGTGTTGTTGGGGAAACCCAAGCAATTGATGGTAGCAAATCAATTTCTACTCTGATTCAATACATAGAAAAGGAGAGATTATTGAAGTGTGTTGAAAAGGTCCAAGAGATGTCATTGAACAGTGTGTTCACTGGCAAGGATTCAAGTGCATCTTCTGTCCCTAGTGTGCCTCAAAGCCCAATGGGTGTGTTGGATACATTGTGCTTCAGCTACAAAAGTGATGACACCAATGCTGGTGGTGGTGGTTCATGTTCTTCTTCACATAGTAGTAGCCCAGATGCTAAAAGGAGGAAGCTAAACAAAACCTGTGGATCAGAGCTTTTGTAG
Protein sequence

>Msa1351030

MAPSFDCVSSLLCTEEDSSVFDDGEYGGGGSVEVYEDLWRSRNDHQMTQQRYDGVPDELPLQSEECLVLMLEKECQQWPGADYLNRLQFGDLDFQARNEVIDWIQKVRAHFGFGPLCAYLSINYLDRFLSVYEFPKGRAWTMQLLAVACLSLAAKVEETAVPQPLDLQIGESKFVFEAKTIQRMELLVLSTLKWRMQAITPFSFIECFLSKINDDDKSSSRFSILRSTQLISSTIKGLDFLEFKPSEIAAAVATCVVGETQAIDGSKSISTLIQYIEKERLLKCVEKVQEMSLNSVFTGKDSSASSVPSVPQSPMGVLDTLCFSYKSDDTNAGGGGSCSSSHSSSPDAKRRKLNKTCGSELL*