AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1353790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1353790 MtrunA17_Chr1g0150371 77.483 151 2 1 10 128 1 151 1.42e-77 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1353790 AT2G24970.1 40.940 149 56 1 12 128 4 152 4.61e-35 119

Find 33 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATATGATAGTAGCTGTTT+TGG 0.249235 tig0014038:-26196 Msa1353790:three_prime_UTR
CTTTCATAGCGAAAACTATT+TGG 0.250674 tig0014038:-26229 Msa1353790:three_prime_UTR
GAACCACAAGATTCTGATTC+TGG 0.302148 tig0014038:-26504 Msa1353790:CDS
GCATCATTCAAAATTAGTTT+TGG 0.306642 tig0014038:-25887 Msa1353790:three_prime_UTR
CCAGTATGTTACACATGTTA+TGG 0.354700 tig0014038:+26047 None:intergenic
CCATAACATGTGTAACATAC+TGG 0.388934 tig0014038:-26047 Msa1353790:three_prime_UTR
AATAGAACTTTGCCGTGTAT+TGG 0.392603 tig0014038:-26337 Msa1353790:three_prime_UTR
ACATACTGGAAAGTTAATTC+AGG 0.415415 tig0014038:-26033 Msa1353790:three_prime_UTR
TCATCATCTTCACTAGTAAC+GGG 0.420623 tig0014038:+29199 None:intergenic
TGCATTCACTAACTTCAAAC+AGG 0.459588 tig0014038:-29176 Msa1353790:intron
GCTACAATATGTAGCTTCAA+TGG 0.460763 tig0014038:+26251 None:intergenic
TTGTGTGCTTCTGCTGTGGA+AGG 0.501500 tig0014038:-26163 Msa1353790:three_prime_UTR
GTGTGCACACAGAAACAGTA+TGG 0.501688 tig0014038:-26117 Msa1353790:three_prime_UTR
ATCATCATCTTCACTAGTAA+CGG 0.530991 tig0014038:+29198 None:intergenic
GTGAGTGTTTCGATCTGTGT+TGG 0.548936 tig0014038:-26292 Msa1353790:three_prime_UTR
TAACTTGTGTGCTTCTGCTG+TGG 0.553563 tig0014038:-26167 Msa1353790:three_prime_UTR
TTCTATCTTGCTCACCACAA+TGG 0.578942 tig0014038:+26073 None:intergenic
TATATATCTGATAAAACCCT+AGG 0.583439 tig0014038:+26383 None:intergenic
ATGTCATATTTACACGAGGA+AGG 0.586125 tig0014038:-25985 Msa1353790:three_prime_UTR
GCAGCGTATGCAATCATCTG+TGG 0.599854 tig0014038:-29227 Msa1353790:CDS
TTGTGCAGGTGATTGATGAA+TGG 0.600689 tig0014038:-27625 Msa1353790:intron
CAGCGTATGCAATCATCTGT+GGG 0.606247 tig0014038:-29226 Msa1353790:CDS
GCACACAGAAACAGTATGGT+AGG 0.608143 tig0014038:-26113 Msa1353790:three_prime_UTR
GAATAGTTTCACAAGTCCGT+CGG 0.620054 tig0014038:+30130 None:intergenic
GTTGAAATTCAGTTTGAAGA+CGG 0.620867 tig0014038:+30072 None:intergenic
ATTTATGAAGTCATAACACT+CGG 0.635098 tig0014038:+26006 None:intergenic
TGTGTGCTTCTGCTGTGGAA+GGG 0.640233 tig0014038:-26162 Msa1353790:three_prime_UTR
TCTCCAGAATCAGAATCTTG+TGG 0.645712 tig0014038:+26501 None:intergenic
CATCATCTTCACTAGTAACG+GGG 0.657093 tig0014038:+29200 None:intergenic
TTGTGAAGGAATGACATGAG+TGG 0.677030 tig0014038:-25927 Msa1353790:three_prime_UTR
GAATACAATGAAGCAACCGA+CGG 0.695465 tig0014038:-30146 Msa1353790:CDS
TAAGAAGAATAACCAATACA+CGG 0.712508 tig0014038:+26325 None:intergenic
ATAAATGTCATATTTACACG+AGG 0.735678 tig0014038:-25989 Msa1353790:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAAGAAAAATGAAAAAA+GGG + tig0014038:26047-26066 None:intergenic 15.0%
!! AATTGAAGAAAAATGAAAAA+AGG + tig0014038:26048-26067 None:intergenic 15.0%
!! ATAATAAGTGGTTAAATATA+GGG - tig0014038:26516-26535 Msa1353790:intron 15.0%
!!! TGACTATGAATATTTTAAAA+AGG + tig0014038:26572-26591 None:intergenic 15.0%
!! TGTCTAATTTATAAGTTATT+TGG - tig0014038:27096-27115 Msa1353790:intron 15.0%
!! GTCTAATTTATAAGTTATTT+GGG - tig0014038:27097-27116 Msa1353790:intron 15.0%
!! TCTAATTTATAAGTTATTTG+GGG - tig0014038:27098-27117 Msa1353790:intron 15.0%
!! TTTAATGTAATTAATGATTG+CGG + tig0014038:27246-27265 None:intergenic 15.0%
!! AACCAATATAAATCAAAATA+CGG - tig0014038:27737-27756 Msa1353790:intron 15.0%
!! CTAAAATTATGTTTATAAGT+GGG - tig0014038:28057-28076 Msa1353790:intron 15.0%
!! AATGATGATTTACATAAATA+AGG + tig0014038:28535-28554 None:intergenic 15.0%
!!! GTTTATTACTTACATTTTCA+GGG + tig0014038:26001-26020 None:intergenic 20.0%
!! TTGAAGAAAAATGAAAAAAG+GGG + tig0014038:26046-26065 None:intergenic 20.0%
!! CATAACATTACTATCAAATT+TGG + tig0014038:26454-26473 None:intergenic 20.0%
!!! CAAATTTGATAGTAATGTTA+TGG - tig0014038:26452-26471 Msa1353790:exon 20.0%
!! TTAACCACTTATTATCAATT+TGG + tig0014038:26511-26530 None:intergenic 20.0%
!! GATAATAAGTGGTTAAATAT+AGG - tig0014038:26515-26534 Msa1353790:intron 20.0%
!!! TTTAAAATATTCATAGTCAC+AGG - tig0014038:26573-26592 Msa1353790:intron 20.0%
!! TTAAAATATTCATAGTCACA+GGG - tig0014038:26574-26593 Msa1353790:intron 20.0%
!!! AAAGGATCAAAAGAATATTT+TGG + tig0014038:27043-27062 None:intergenic 20.0%
!!! ATAGAGTTTTGTTTGTTTAA+AGG + tig0014038:27061-27080 None:intergenic 20.0%
!! AAAATCTAGCAAAAATACTT+AGG + tig0014038:27544-27563 None:intergenic 20.0%
!! CTGAAAATATCTTTATAAGT+GGG - tig0014038:27856-27875 Msa1353790:intron 20.0%
!!! CCACTTATAAACATAATTTT+AGG + tig0014038:28059-28078 None:intergenic 20.0%
!! CCTAAAATTATGTTTATAAG+TGG - tig0014038:28056-28075 Msa1353790:intron 20.0%
!! ATTTATGTAAATCATCATTC+TGG - tig0014038:28536-28555 Msa1353790:intron 20.0%
!!! TTGTTATTTATTACTCTAGT+TGG - tig0014038:28777-28796 Msa1353790:intron 20.0%
!! GGTTTATTACTTACATTTTC+AGG + tig0014038:26002-26021 None:intergenic 25.0%
! TGAAGAAAAATGAAAAAAGG+GGG + tig0014038:26045-26064 None:intergenic 25.0%
!! AGAAGAGATTTCAATTTTGA+AGG - tig0014038:26163-26182 Msa1353790:three_prime_UTR 25.0%
!! GAAGAGATTTCAATTTTGAA+GGG - tig0014038:26164-26183 Msa1353790:three_prime_UTR 25.0%
!!! AAGAGATTTCAATTTTGAAG+GGG - tig0014038:26165-26184 Msa1353790:three_prime_UTR 25.0%
!! TAGACCAAATTGATAATAAG+TGG - tig0014038:26504-26523 Msa1353790:CDS 25.0%
! ATGCATACATAAATGAGTAA+AGG + tig0014038:27000-27019 None:intergenic 25.0%
! ATTACATTAAAATGTTGTCG+TGG - tig0014038:27255-27274 Msa1353790:intron 25.0%
! TTACATTAAAATGTTGTCGT+GGG - tig0014038:27256-27275 Msa1353790:intron 25.0%
! AAAAATACTTAGGAGAGATT+CGG + tig0014038:27534-27553 None:intergenic 25.0%
!!! TTGCTAGATTTTGATTTCAT+AGG - tig0014038:27552-27571 Msa1353790:intron 25.0%
!!! CTCCGTATTTTGATTTATAT+TGG + tig0014038:27742-27761 None:intergenic 25.0%
! TAAGTATTTAACCAAGCTTT+GGG - tig0014038:27767-27786 Msa1353790:intron 25.0%
!! CCACTTATAAAGATATTTTC+AGG + tig0014038:27858-27877 None:intergenic 25.0%
! CCTGAAAATATCTTTATAAG+TGG - tig0014038:27855-27874 Msa1353790:intron 25.0%
! AAATTATGTTTATAAGTGGG+AGG - tig0014038:28060-28079 Msa1353790:intron 25.0%
! AGTTGAGAGATACAAAATTT+GGG + tig0014038:28324-28343 None:intergenic 25.0%
! AAGTTGAGAGATACAAAATT+TGG + tig0014038:28325-28344 None:intergenic 25.0%
! GATCAAATAAGATCAAAAAC+TGG - tig0014038:28441-28460 Msa1353790:intron 25.0%
!!! TTTTCTAGCTATTAAAACAG+AGG - tig0014038:28569-28588 Msa1353790:intron 25.0%
! AAAGAGATAACAACAGATTA+TGG - tig0014038:28616-28635 Msa1353790:intron 25.0%
!! TTTCTAGGAGAGTAAAAAAA+GGG + tig0014038:28649-28668 None:intergenic 25.0%
! TTATTTATTACTCTAGTTGG+TGG - tig0014038:28780-28799 Msa1353790:intron 25.0%
! ACAACACTTTATACTAAACA+GGG + tig0014038:29050-29069 None:intergenic 25.0%
!!! TATAAAGTGTTGTCAAATAG+CGG - tig0014038:29057-29076 Msa1353790:intron 25.0%
! TTGTATTACATATTGTATCG+CGG + tig0014038:29267-29286 None:intergenic 25.0%
! ACAATATGTAATACAAAGTG+TGG - tig0014038:29271-29290 Msa1353790:CDS 25.0%
! TACACTACTAGATTAAAGTT+AGG - tig0014038:29383-29402 Msa1353790:intron 25.0%
! GAAATGGTATACAAATGAAA+AGG + tig0014038:29458-29477 None:intergenic 25.0%
! TATATATCTGATAAAACCCT+AGG + tig0014038:29660-29679 None:intergenic 25.0%
!!! AGGGTTTTATCAGATATATA+TGG - tig0014038:29660-29679 Msa1353790:intron 25.0%
! TAAGAAGAATAACCAATACA+CGG + tig0014038:29718-29737 None:intergenic 25.0%
!! AAATATGATAGTAGCTGTTT+TGG - tig0014038:29844-29863 Msa1353790:intron 25.0%
!!! AATATGATAGTAGCTGTTTT+GGG - tig0014038:29845-29864 Msa1353790:intron 25.0%
! ATTTATGAAGTCATAACACT+CGG + tig0014038:30037-30056 None:intergenic 25.0%
! ATAAATGTCATATTTACACG+AGG - tig0014038:30051-30070 Msa1353790:intron 25.0%
!!! GCATCATTCAAAATTAGTTT+TGG - tig0014038:30153-30172 Msa1353790:CDS 25.0%
GTTGAAATTCAGTTTGAAGA+CGG + tig0014038:25971-25990 None:intergenic 30.0%
TCTTCAATTCCTGTGATAAT+TGG - tig0014038:26059-26078 Msa1353790:three_prime_UTR 30.0%
!! AAAAAGGTACCAATTATCAC+AGG + tig0014038:26071-26090 None:intergenic 30.0%
ATCAGTGTTAAGCACAAAAA+AGG + tig0014038:26087-26106 None:intergenic 30.0%
TTTGTGCTTAACACTGATAT+TGG - tig0014038:26089-26108 Msa1353790:three_prime_UTR 30.0%
ATAGAAAACCATGTCGAATT+CGG + tig0014038:26330-26349 None:intergenic 30.0%
TATGACACTCTCAATTCAAA+CGG - tig0014038:26347-26366 Msa1353790:three_prime_UTR 30.0%
GGATCAACTTGACAATTTAA+TGG - tig0014038:26537-26556 Msa1353790:intron 30.0%
ATTCATATGTTAGTGTGAGA+TGG - tig0014038:26692-26711 Msa1353790:intron 30.0%
TTCATATGTTAGTGTGAGAT+GGG - tig0014038:26693-26712 Msa1353790:intron 30.0%
ATCATCATCTTCACTAGTAA+CGG + tig0014038:26845-26864 None:intergenic 30.0%
GTATGCATAATGATTCATGT+TGG - tig0014038:27012-27031 Msa1353790:intron 30.0%
CAACATAAGATTATCGTTCA+AGG - tig0014038:27206-27225 Msa1353790:intron 30.0%
TACATTAAAATGTTGTCGTG+GGG - tig0014038:27257-27276 Msa1353790:intron 30.0%
AATTGTGATCACTATGCATT+TGG - tig0014038:27366-27385 Msa1353790:intron 30.0%
! TTTGGTGATCTTTGATGTAA+AGG - tig0014038:27384-27403 Msa1353790:intron 30.0%
! TTGGTGATCTTTGATGTAAA+GGG - tig0014038:27385-27404 Msa1353790:intron 30.0%
AAAAAGCTGAAAACGACTTA+TGG - tig0014038:27432-27451 Msa1353790:intron 30.0%
AAAAGCTGAAAACGACTTAT+GGG - tig0014038:27433-27452 Msa1353790:intron 30.0%
!! CTCGTGAGTTAAGTTTTAAT+AGG - tig0014038:27503-27522 Msa1353790:intron 30.0%
!!! GAATCTTTTGCTATTCTCAA+AGG - tig0014038:27663-27682 Msa1353790:intron 30.0%
! GATTTATATTGGTTGTTTGC+TGG + tig0014038:27731-27750 None:intergenic 30.0%
GTAAGTATTTAACCAAGCTT+TGG - tig0014038:27766-27785 Msa1353790:intron 30.0%
TATAAGCAAAGCAATGTGAA+AGG + tig0014038:27795-27814 None:intergenic 30.0%
TCACATTGCTTTGCTTATAT+AGG - tig0014038:27796-27815 Msa1353790:intron 30.0%
CACATTGCTTTGCTTATATA+GGG - tig0014038:27797-27816 Msa1353790:intron 30.0%
GGTTATATCTCATCCAATAT+GGG + tig0014038:27837-27856 None:intergenic 30.0%
AGGTTATATCTCATCCAATA+TGG + tig0014038:27838-27857 None:intergenic 30.0%
AAAATTTAAGCCCTACAAAC+TGG + tig0014038:27903-27922 None:intergenic 30.0%
! AAGTCCAACCAAATTTTAAG+AGG - tig0014038:28125-28144 Msa1353790:intron 30.0%
AACTAGAGTAATAGCTCAAA+CGG - tig0014038:28221-28240 Msa1353790:intron 30.0%
TGAAAACCATTGCAATTACT+TGG + tig0014038:28380-28399 None:intergenic 30.0%
!!! ACAATTTTAACTGATTGTGC+AGG - tig0014038:28401-28420 Msa1353790:intron 30.0%
TAGCTATTAAAACAGAGGTT+TGG - tig0014038:28574-28593 Msa1353790:intron 30.0%
GATAACAACAGATTATGGTT+TGG - tig0014038:28621-28640 Msa1353790:intron 30.0%
! TTCTAGGAGAGTAAAAAAAG+GGG + tig0014038:28648-28667 None:intergenic 30.0%
! GTTTCTAGGAGAGTAAAAAA+AGG + tig0014038:28650-28669 None:intergenic 30.0%
TTAAAAACTATCCTAGAAGC+TGG - tig0014038:28747-28766 Msa1353790:intron 30.0%
ACAAAAATATTCCAGCTTCT+AGG + tig0014038:28761-28780 None:intergenic 30.0%
AGATGCATTAGTTAGTCATA+AGG - tig0014038:28875-28894 Msa1353790:intron 30.0%
TTGTCAAATAGCAGTTATAG+TGG - tig0014038:28967-28986 Msa1353790:intron 30.0%
GACAACACTTTATACTAAAC+AGG + tig0014038:29051-29070 None:intergenic 30.0%
GAGTGTAATAACAGAATATG+AGG - tig0014038:29167-29186 Msa1353790:intron 30.0%
TGTACCAATGTTGTTAATCA+CGG + tig0014038:29367-29386 None:intergenic 30.0%
TGGTATACAAATGAAAAGGA+TGG + tig0014038:29454-29473 None:intergenic 30.0%
! GCATTTCTGAATTCTAGAAA+TGG + tig0014038:29474-29493 None:intergenic 30.0%
!!! CTGTAACATGCATGATTTTT+TGG + tig0014038:29624-29643 None:intergenic 30.0%
CTTTCATAGCGAAAACTATT+TGG - tig0014038:29811-29830 Msa1353790:intron 30.0%
AAAAATAGCATCAACCATTG+TGG - tig0014038:29953-29972 Msa1353790:intron 30.0%
ACATACTGGAAAGTTAATTC+AGG - tig0014038:30007-30026 Msa1353790:intron 30.0%
!! TGAAGCTTTTGAGAAGCAAA+TGG - tig0014038:25866-25885 Msa1353790:three_prime_UTR 35.0%
!! GAAGCTTTTGAGAAGCAAAT+GGG - tig0014038:25867-25886 Msa1353790:three_prime_UTR 35.0%
!! AAGCTTTTGAGAAGCAAATG+GGG - tig0014038:25868-25887 Msa1353790:three_prime_UTR 35.0%
TCCTAGAAACAAGCTTCATA+GGG + tig0014038:26130-26149 None:intergenic 35.0%
ATCCTAGAAACAAGCTTCAT+AGG + tig0014038:26131-26150 None:intergenic 35.0%
CTTTAGATTGAAGACGTGTT+TGG - tig0014038:26260-26279 Msa1353790:three_prime_UTR 35.0%
ATGACACTCTCAATTCAAAC+GGG - tig0014038:26348-26367 Msa1353790:three_prime_UTR 35.0%
AAATTAGTCCCTGACATTGT+AGG + tig0014038:26616-26635 None:intergenic 35.0%
TACAATGTCAGGGACTAATT+TGG - tig0014038:26615-26634 Msa1353790:intron 35.0%
TCATATGTTAGTGTGAGATG+GGG - tig0014038:26694-26713 Msa1353790:intron 35.0%
TCATCATCTTCACTAGTAAC+GGG + tig0014038:26844-26863 None:intergenic 35.0%
TGCATTCACTAACTTCAAAC+AGG - tig0014038:26864-26883 Msa1353790:intron 35.0%
AAGAACACGCATTCAACTAT+AGG + tig0014038:26942-26961 None:intergenic 35.0%
TGAAATGCCACACACAAAAA+AGG + tig0014038:26972-26991 None:intergenic 35.0%
GTTGTAAACGCAAAAAGAAG+AGG - tig0014038:27136-27155 Msa1353790:intron 35.0%
TAAACGCAAAAAGAAGAGGT+GGG - tig0014038:27140-27159 Msa1353790:intron 35.0%
TAGCTATTGTTTCGTCTTTG+TGG - tig0014038:27177-27196 Msa1353790:intron 35.0%
! CGAGTTGTTCATTTTCTGAT+TGG - tig0014038:27309-27328 Msa1353790:intron 35.0%
! GTTGTTCATTTTCTGATTGG+TGG - tig0014038:27312-27331 Msa1353790:intron 35.0%
TGATCTTTGATGTAAAGGGT+TGG - tig0014038:27389-27408 Msa1353790:intron 35.0%
AGATGCAGTCTCTTCTATTA+TGG - tig0014038:27603-27622 Msa1353790:CDS 35.0%
!! TTAAAACACCGCTTTGAAAC+TGG + tig0014038:27692-27711 None:intergenic 35.0%
TTGCTTTGCTTATATAGGGA+AGG - tig0014038:27801-27820 Msa1353790:intron 35.0%
TGCTTTGCTTATATAGGGAA+GGG - tig0014038:27802-27821 Msa1353790:intron 35.0%
ACATTAGAAGCCAGTTTGTA+GGG - tig0014038:27890-27909 Msa1353790:intron 35.0%
AAAGCTTATCCAAGATCTGA+CGG - tig0014038:27934-27953 Msa1353790:intron 35.0%
AGTAGCTCAAAATTGACAGT+GGG + tig0014038:27964-27983 None:intergenic 35.0%
AAGTAGCTCAAAATTGACAG+TGG + tig0014038:27965-27984 None:intergenic 35.0%
! ACTGTCAATTTTGAGCTACT+TGG - tig0014038:27964-27983 Msa1353790:intron 35.0%
! CTGTCAATTTTGAGCTACTT+GGG - tig0014038:27965-27984 Msa1353790:intron 35.0%
TTAATTCAACCCTGCAAAAC+CGG + tig0014038:28107-28126 None:intergenic 35.0%
AGGACCTCTTAAAATTTGGT+TGG + tig0014038:28132-28151 None:intergenic 35.0%
CAACAGGACCTCTTAAAATT+TGG + tig0014038:28136-28155 None:intergenic 35.0%
!! CAAATTTTAAGAGGTCCTGT+TGG - tig0014038:28134-28153 Msa1353790:intron 35.0%
ACTAGAGTAATAGCTCAAAC+GGG - tig0014038:28222-28241 Msa1353790:intron 35.0%
AAAATCACTTTGCAGATGCT+TGG + tig0014038:28298-28317 None:intergenic 35.0%
AGTCAACCAAGTAATTGCAA+TGG - tig0014038:28371-28390 Msa1353790:intron 35.0%
! AAAACTGGTGAGCAGTTTTA+CGG - tig0014038:28456-28475 Msa1353790:intron 35.0%
TCCTAGAAACTAATCCACTT+GGG - tig0014038:28660-28679 Msa1353790:intron 35.0%
TTACACAAACAAAAGCGAAG+AGG + tig0014038:28840-28859 None:intergenic 35.0%
TAGTTAGTCATAAGGTCTGT+AGG - tig0014038:28883-28902 Msa1353790:intron 35.0%
AAATAGCAGTTATAGTGGCT+CGG - tig0014038:28972-28991 Msa1353790:intron 35.0%
TCAAATTCCTCAACACTAGA+GGG + tig0014038:29099-29118 None:intergenic 35.0%
TTCAAATTCCTCAACACTAG+AGG + tig0014038:29100-29119 None:intergenic 35.0%
GTATGCATGTCATGTTTATC+TGG - tig0014038:29199-29218 Msa1353790:CDS 35.0%
TACAAAGTGTGGTCAAATAG+TGG - tig0014038:29282-29301 Msa1353790:intron 35.0%
!!! CTGTAAATGTGTTTTGATGC+AGG - tig0014038:29509-29528 Msa1353790:intron 35.0%
!!! TGCATGATTTTTTGGTTGTC+TGG + tig0014038:29616-29635 None:intergenic 35.0%
!!! ACAGTTTTAGTTCAGTTCCT+AGG - tig0014038:29640-29659 Msa1353790:intron 35.0%
!!! CAGTTTTAGTTCAGTTCCTA+GGG - tig0014038:29641-29660 Msa1353790:intron 35.0%
AATAGAACTTTGCCGTGTAT+TGG - tig0014038:29703-29722 Msa1353790:intron 35.0%
GCTACAATATGTAGCTTCAA+TGG + tig0014038:29792-29811 None:intergenic 35.0%
CCAGTATGTTACACATGTTA+TGG + tig0014038:29996-30015 None:intergenic 35.0%
CCATAACATGTGTAACATAC+TGG - tig0014038:29993-30012 Msa1353790:intron 35.0%
ATGTCATATTTACACGAGGA+AGG - tig0014038:30055-30074 Msa1353790:CDS 35.0%
GAATACAATGAAGCAACCGA+CGG - tig0014038:25894-25913 Msa1353790:three_prime_UTR 40.0%
GAATAGTTTCACAAGTCCGT+CGG + tig0014038:25913-25932 None:intergenic 40.0%
! GTGAAAAGTGTAATATGGGG+GGG + tig0014038:26023-26042 None:intergenic 40.0%
! GGTGAAAAGTGTAATATGGG+GGG + tig0014038:26024-26043 None:intergenic 40.0%
! GGGTGAAAAGTGTAATATGG+GGG + tig0014038:26025-26044 None:intergenic 40.0%
! GGGGTGAAAAGTGTAATATG+GGG + tig0014038:26026-26045 None:intergenic 40.0%
! GGGGGTGAAAAGTGTAATAT+GGG + tig0014038:26027-26046 None:intergenic 40.0%
! AGGGGGTGAAAAGTGTAATA+TGG + tig0014038:26028-26047 None:intergenic 40.0%
!!! CACTGATATTGGTTTTCTGC+AGG - tig0014038:26100-26119 Msa1353790:three_prime_UTR 40.0%
! ACCCTATGAAGCTTGTTTCT+AGG - tig0014038:26126-26145 Msa1353790:three_prime_UTR 40.0%
AATGTCAGGGACTAATTTGG+TGG - tig0014038:26618-26637 Msa1353790:intron 40.0%
CATCATCTTCACTAGTAACG+GGG + tig0014038:26843-26862 None:intergenic 40.0%
!!! TGATGTGCCTTTTTTGTGTG+TGG - tig0014038:26962-26981 Msa1353790:intron 40.0%
GTAAACGCAAAAAGAAGAGG+TGG - tig0014038:27139-27158 Msa1353790:intron 40.0%
TTAAAATGTTGTCGTGGGGA+TGG - tig0014038:27261-27280 Msa1353790:intron 40.0%
GGATGGTTTATTGGTAGAGA+AGG - tig0014038:27278-27297 Msa1353790:intron 40.0%
GGTTTATTGGTAGAGAAGGA+AGG - tig0014038:27282-27301 Msa1353790:intron 40.0%
AATAGCCAAGTGAAGAGTGA+TGG - tig0014038:27628-27647 Msa1353790:intron 40.0%
AGTTACCATCACTCTTCACT+TGG + tig0014038:27636-27655 None:intergenic 40.0%
AAAGGTTGCCAGTTTCAAAG+CGG - tig0014038:27681-27700 Msa1353790:intron 40.0%
GCTTTGCTTATATAGGGAAG+GGG - tig0014038:27803-27822 Msa1353790:intron 40.0%
ATCTCATCCAATATGGGAAC+AGG + tig0014038:27831-27850 None:intergenic 40.0%
! CAAACTGGCTTCTAATGTGA+GGG + tig0014038:27888-27907 None:intergenic 40.0%
! ACAAACTGGCTTCTAATGTG+AGG + tig0014038:27889-27908 None:intergenic 40.0%
CACATTAGAAGCCAGTTTGT+AGG - tig0014038:27889-27908 Msa1353790:intron 40.0%
AAGCTTATCCAAGATCTGAC+GGG - tig0014038:27935-27954 Msa1353790:intron 40.0%
AAGACTGAACATTTGCAGCA+TGG + tig0014038:27991-28010 None:intergenic 40.0%
TGCTGCAAATGTTCAGTCTT+AGG - tig0014038:27991-28010 Msa1353790:intron 40.0%
GTTCCTCATTGGATGACATA+TGG - tig0014038:28033-28052 Msa1353790:intron 40.0%
!!! AGGTCCTGTTGGTTTTTAGT+TGG - tig0014038:28145-28164 Msa1353790:intron 40.0%
TCTGCCAACTAAAAACCAAC+AGG + tig0014038:28152-28171 None:intergenic 40.0%
!!! GTTTTTAGTTGGCAGAACGT+GGG - tig0014038:28156-28175 Msa1353790:intron 40.0%
AGGGAGAAGGTAAAATACAG+AGG - tig0014038:28180-28199 Msa1353790:intron 40.0%
TTGTGCAGGTGATTGATGAA+TGG - tig0014038:28415-28434 Msa1353790:intron 40.0%
! GAGCAGTTTTACGGACTTTT+TGG - tig0014038:28465-28484 Msa1353790:intron 40.0%
TCAAATGTACAGCATGTGCA+TGG + tig0014038:28503-28522 None:intergenic 40.0%
CTCCTAGAAACTAATCCACT+TGG - tig0014038:28659-28678 Msa1353790:intron 40.0%
!! ACCCAAGTGGATTAGTTTCT+AGG + tig0014038:28664-28683 None:intergenic 40.0%
AGAAACTAATCCACTTGGGT+TGG - tig0014038:28664-28683 Msa1353790:intron 40.0%
CAAAAGCGAAGAGGAGAAAA+AGG + tig0014038:28831-28850 None:intergenic 40.0%
TCGATTCCTTCAATGAAGAG+AGG - tig0014038:28918-28937 Msa1353790:intron 40.0%
TTATACTAAACAGGGTGACG+CGG + tig0014038:29042-29061 None:intergenic 40.0%
! ACATCAAGTGTTGTCAATCG+TGG + tig0014038:29144-29163 None:intergenic 40.0%
TGCATGTCATGTTTATCTGG+CGG - tig0014038:29202-29221 Msa1353790:CDS 40.0%
CCAATGTTGTTAATCACGGA+TGG + tig0014038:29363-29382 None:intergenic 40.0%
CCATCCGTGATTAACAACAT+TGG - tig0014038:29360-29379 Msa1353790:intron 40.0%
GAACCACAAGATTCTGATTC+TGG - tig0014038:29536-29555 Msa1353790:intron 40.0%
TCTCCAGAATCAGAATCTTG+TGG + tig0014038:29542-29561 None:intergenic 40.0%
TTCTATCTTGCTCACCACAA+TGG + tig0014038:29970-29989 None:intergenic 40.0%
TTGTGAAGGAATGACATGAG+TGG - tig0014038:30113-30132 Msa1353790:CDS 40.0%
GTAAACCACCGAATTCGACA+TGG - tig0014038:26319-26338 Msa1353790:three_prime_UTR 45.0%
GAAAACCATGTCGAATTCGG+TGG + tig0014038:26327-26346 None:intergenic 45.0%
GACAGAGTCAGTGACATTTG+TGG + tig0014038:26394-26413 None:intergenic 45.0%
! TATCAGTCCTTCTGAGAGTG+CGG - tig0014038:26426-26445 Msa1353790:three_prime_UTR 45.0%
ATTGTAGGCCACTGTCGAAT+AGG + tig0014038:26601-26620 None:intergenic 45.0%
GTGAGATGGGGATAAGATCT+AGG - tig0014038:26706-26725 Msa1353790:intron 45.0%
CAGCGTATGCAATCATCTGT+GGG - tig0014038:26814-26833 Msa1353790:intron 45.0%
AAGAGGTGGGACATGTCATA+CGG - tig0014038:27153-27172 Msa1353790:intron 45.0%
TTGTCGTGGGGATGGTTTAT+TGG - tig0014038:27269-27288 Msa1353790:intron 45.0%
! TTGGTTGTTTGCTGGCAAGA+GGG + tig0014038:27723-27742 None:intergenic 45.0%
! ATTGGTTGTTTGCTGGCAAG+AGG + tig0014038:27724-27743 None:intergenic 45.0%
!! TGTGAAAGGTACCCAAAGCT+TGG + tig0014038:27781-27800 None:intergenic 45.0%
AATGTTCAGTCTTAGGCGTG+AGG - tig0014038:27998-28017 Msa1353790:intron 45.0%
ATGTTCAGTCTTAGGCGTGA+GGG - tig0014038:27999-28018 Msa1353790:intron 45.0%
AGGCCATATGTCATCCAATG+AGG + tig0014038:28039-28058 None:intergenic 45.0%
!!! GGTTTTTAGTTGGCAGAACG+TGG - tig0014038:28155-28174 Msa1353790:intron 45.0%
ACAGCATGTGCATGGACAAT+TGG + tig0014038:28495-28514 None:intergenic 45.0%
!! TAAGGTCTGTAGGAGTGCTT+AGG - tig0014038:28893-28912 Msa1353790:intron 45.0%
!! AAGGTCTGTAGGAGTGCTTA+GGG - tig0014038:28894-28913 Msa1353790:intron 45.0%
CACTCTCCTCTCTTCATTGA+AGG + tig0014038:28927-28946 None:intergenic 45.0%
CTTCAATGAAGAGAGGAGAG+TGG - tig0014038:28925-28944 Msa1353790:intron 45.0%
TAGTGGCTATAGTGCTGTAG+CGG - tig0014038:29299-29318 Msa1353790:intron 45.0%
GTGAGTGTTTCGATCTGTGT+TGG - tig0014038:29748-29767 Msa1353790:intron 45.0%
! TAACTTGTGTGCTTCTGCTG+TGG - tig0014038:29873-29892 Msa1353790:intron 45.0%
GTGTGCACACAGAAACAGTA+TGG - tig0014038:29923-29942 Msa1353790:intron 45.0%
GCACACAGAAACAGTATGGT+AGG - tig0014038:29927-29946 Msa1353790:intron 45.0%
! CTGCAAGCTGCATTTTGTGA+AGG - tig0014038:30099-30118 Msa1353790:CDS 45.0%
TGAGCTTCTTGCTGCTAAGC+AGG - tig0014038:26196-26215 Msa1353790:three_prime_UTR 50.0%
! ATCAGTCCTTCTGAGAGTGC+GGG - tig0014038:26427-26446 Msa1353790:three_prime_UTR 50.0%
GACAGTGGCCTACAATGTCA+GGG - tig0014038:26605-26624 Msa1353790:intron 50.0%
! GTTTGCTTGTTGAGCTGTGC+AGG - tig0014038:26741-26760 Msa1353790:intron 50.0%
GCAGCGTATGCAATCATCTG+TGG - tig0014038:26813-26832 Msa1353790:intron 50.0%
AGGGGAACCTGTTCCCATAT+TGG - tig0014038:27821-27840 Msa1353790:intron 50.0%
TGTTCAGTCTTAGGCGTGAG+GGG - tig0014038:28000-28019 Msa1353790:intron 50.0%
GTGCGTTGAGTGTTCCTCAT+TGG - tig0014038:28022-28041 Msa1353790:intron 50.0%
TGCAAAACCGGCAGCTTGTA+AGG + tig0014038:28095-28114 None:intergenic 50.0%
!!! TTACAAGCTGCCGGTTTTGC+AGG - tig0014038:28094-28113 Msa1353790:intron 50.0%
!!! TACAAGCTGCCGGTTTTGCA+GGG - tig0014038:28095-28114 Msa1353790:intron 50.0%
TTAGTTGGCAGAACGTGGGA+AGG - tig0014038:28160-28179 Msa1353790:intron 50.0%
TAGTTGGCAGAACGTGGGAA+GGG - tig0014038:28161-28180 Msa1353790:intron 50.0%
GATCTCAAGCCAACACCACT+AGG + tig0014038:28690-28709 None:intergenic 50.0%
!! GTGGTGTTGGCTTGAGATCT+TGG - tig0014038:28691-28710 Msa1353790:intron 50.0%
CTTGGAGTGTGCTCTTCTCA+AGG - tig0014038:28709-28728 Msa1353790:intron 50.0%
TGCTCAACCCTCTAGTGTTG+AGG - tig0014038:29089-29108 Msa1353790:intron 50.0%
GTGTTGTCAATCGTGGAACG+TGG + tig0014038:29137-29156 None:intergenic 50.0%
GCAGCGAAATGAAGACAGCA+TGG + tig0014038:29421-29440 None:intergenic 50.0%
GCTGTCTTCATTTCGCTGCA+TGG - tig0014038:29422-29441 Msa1353790:intron 50.0%
TTGTGTGCTTCTGCTGTGGA+AGG - tig0014038:29877-29896 Msa1353790:intron 50.0%
TGTGTGCTTCTGCTGTGGAA+GGG - tig0014038:29878-29897 Msa1353790:intron 50.0%
TTTGGTCCCGCACTCTCAGA+AGG + tig0014038:26436-26455 None:intergenic 55.0%
CACAGGGACCTATTCGACAG+TGG - tig0014038:26590-26609 Msa1353790:intron 55.0%
CGACAGTGGCCTACAATGTC+AGG - tig0014038:26604-26623 Msa1353790:intron 55.0%
TTCCTCGCCTTACAAGCTGC+CGG - tig0014038:28085-28104 Msa1353790:intron 55.0%
!! ATCCACTTGGGTTGGCCTAG+TGG - tig0014038:28672-28691 Msa1353790:intron 55.0%
!! TTGGGTTGGCCTAGTGGTGT+TGG - tig0014038:28678-28697 Msa1353790:intron 55.0%
! ATAGTGGCTCGGCAGTGTTG+TGG - tig0014038:28983-29002 Msa1353790:intron 55.0%
! GTGGGTTGCCCGTCAGATCT+TGG + tig0014038:27946-27965 None:intergenic 60.0%
AACCGGCAGCTTGTAAGGCG+AGG + tig0014038:28090-28109 None:intergenic 60.0%
GCAGAACGTGGGAAGGGAGA+AGG - tig0014038:28167-28186 Msa1353790:intron 60.0%
CACCACTAGGCCAACCCAAG+TGG + tig0014038:28677-28696 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0014038 gene 25858 30204 25858 ID=Msa1353790;Name=Msa1353790
tig0014038 mRNA 25858 30204 25858 ID=Msa1353790-mRNA-1;Parent=Msa1353790;Name=Msa1353790-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.66|0.75|1|0.33|0.75|4|600|128
tig0014038 exon 25858 26531 25858 ID=Msa1353790-mRNA-1:exon:8814;Parent=Msa1353790-mRNA-1
tig0014038 exon 27600 27639 27600 ID=Msa1353790-mRNA-1:exon:8813;Parent=Msa1353790-mRNA-1
tig0014038 exon 29177 29299 29177 ID=Msa1353790-mRNA-1:exon:8812;Parent=Msa1353790-mRNA-1
tig0014038 exon 30055 30204 30055 ID=Msa1353790-mRNA-1:exon:8811;Parent=Msa1353790-mRNA-1
tig0014038 CDS 30055 30204 30055 ID=Msa1353790-mRNA-1:cds;Parent=Msa1353790-mRNA-1
tig0014038 CDS 29177 29299 29177 ID=Msa1353790-mRNA-1:cds;Parent=Msa1353790-mRNA-1
tig0014038 CDS 27600 27639 27600 ID=Msa1353790-mRNA-1:cds;Parent=Msa1353790-mRNA-1
tig0014038 CDS 26458 26531 26458 ID=Msa1353790-mRNA-1:cds;Parent=Msa1353790-mRNA-1
tig0014038 three_prime_UTR 25858 26457 25858 ID=Msa1353790-mRNA-1:three_prime_utr;Parent=Msa1353790-mRNA-1
Gene Sequence

>Msa1353790

TGGAAGTTTGAAGCTTTTGAGAAGCAAATGGGGAATGAATACAATGAAGCAACCGACGGACTTGTGAAACTATTCAGAAAAGCAAATCACGATCTTGATATCGTTCAACACCGTCTTCAAACTGAATTTCAACAGCAGTACCCTGAAAATGATTTGATTGATAAAGCTCAGACAGTTCTTGTTGAGAATAGAAATTTAGTGCAGCGTATGCAATCATCTGTGGGAATCCCCGTTACTAGTGAAGATGATGATGCATTCACTAACTTCAAACAGGTGATTGATGAATGGACTGATCAAATAAGATCAAAAACTGGAAATGAACCACAAGATTCTGATTCTGGAGATCTCAACAAACTACTCTTTTCAGCTATAGTTCAAAGTAATTGA
Protein sequence

>Msa1353790

WKFEAFEKQMGNEYNEATDGLVKLFRKANHDLDIVQHRLQTEFQQQYPENDLIDKAQTVLVENRNLVQRMQSSVGIPVTSEDDDAFTNFKQVIDEWTDQIRSKTGNEPQDSDSGDLNKLLFSAIVQSN*