AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0054160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054160 MtrunA17_Chr1g0150371 77.483 151 2 1 10 128 1 151 1.42e-77 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054160 AT2G24970.1 40.940 149 56 1 12 128 4 152 4.61e-35 119

Find 33 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATATGATAGTAGCTGTTT+TGG 0.249235 1_2:-8837550 Msa0054160:three_prime_UTR
CTTTCATAGCGAAAACTATT+TGG 0.250674 1_2:-8837583 Msa0054160:three_prime_UTR
GCATCATTCAAAATTAGTTT+TGG 0.290764 1_2:-8837241 Msa0054160:three_prime_UTR
GAACCACAAGATTCTGATTC+TGG 0.302148 1_2:-8837858 Msa0054160:CDS
CCAGTATGTTACACATGTTA+TGG 0.354700 1_2:+8837401 None:intergenic
CCATAACATGTGTAACATAC+TGG 0.388934 1_2:-8837401 Msa0054160:three_prime_UTR
AATAGAACTTTGCCGTGTAT+TGG 0.392603 1_2:-8837691 Msa0054160:three_prime_UTR
ACATACTGGAAAGTTAATTC+AGG 0.415415 1_2:-8837387 Msa0054160:three_prime_UTR
TCATCATCTTCACTAGTAAC+GGG 0.420623 1_2:+8840550 None:intergenic
TGCATTCACTAACTTCAAAC+AGG 0.459588 1_2:-8840527 Msa0054160:intron
GCTACAATATGTAGCTTCAA+TGG 0.460763 1_2:+8837605 None:intergenic
TTGTGTGCTTCTGCTGTGGA+AGG 0.483363 1_2:-8837517 Msa0054160:three_prime_UTR
GTGTGCACACAGAAACAGTA+TGG 0.501688 1_2:-8837471 Msa0054160:three_prime_UTR
TAATTTGTGTGCTTCTGCTG+TGG 0.524043 1_2:-8837521 Msa0054160:three_prime_UTR
ATCATCATCTTCACTAGTAA+CGG 0.530991 1_2:+8840549 None:intergenic
GTGAGTGTTTCGATCTGTGT+TGG 0.548936 1_2:-8837646 Msa0054160:three_prime_UTR
TTCTATCTTGCTCACCACAA+TGG 0.578942 1_2:+8837427 None:intergenic
TATATATCTGATAAAACCCT+AGG 0.583849 1_2:+8837737 None:intergenic
ATGTCATATTTACACGAGGA+AGG 0.586125 1_2:-8837339 Msa0054160:three_prime_UTR
GCAGCGTATGCAATCATCTG+TGG 0.599854 1_2:-8840578 Msa0054160:CDS
TTGTGCAGGTGATTGATGAA+TGG 0.600689 1_2:-8838979 Msa0054160:intron
CAGCGTATGCAATCATCTGT+GGG 0.606247 1_2:-8840577 Msa0054160:CDS
GCACACAGAAACAGTATGGT+AGG 0.608143 1_2:-8837467 Msa0054160:three_prime_UTR
TGTGTGCTTCTGCTGTGGAA+GGG 0.613009 1_2:-8837516 Msa0054160:three_prime_UTR
GAATAGTTTCACAAGTCCGT+CGG 0.620054 1_2:+8841481 None:intergenic
GTTGAAATTCAGTTTGAAGA+CGG 0.620867 1_2:+8841423 None:intergenic
ATTTATGAAGTCATAACACT+CGG 0.635098 1_2:+8837360 None:intergenic
TCTCCAGAATCAGAATCTTG+TGG 0.645712 1_2:+8837855 None:intergenic
CATCATCTTCACTAGTAACG+GGG 0.657093 1_2:+8840551 None:intergenic
TTGTGAAGGAATGACATGAG+TGG 0.677030 1_2:-8837281 Msa0054160:three_prime_UTR
GAATACAATGAAGCAACCGA+CGG 0.695465 1_2:-8841497 Msa0054160:CDS
TAAGAAGAATAACCAATACA+CGG 0.712508 1_2:+8837679 None:intergenic
ATAAATGTCATATTTACACG+AGG 0.735678 1_2:-8837343 Msa0054160:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAAGAAAAATGAAAAAA+GGG + chr1_2:8837401-8837420 None:intergenic 15.0%
!! AATTGAAGAAAAATGAAAAA+AGG + chr1_2:8837402-8837421 None:intergenic 15.0%
!! ATAATAAGTGGTTAAATATA+GGG - chr1_2:8837870-8837889 Msa0054160:intron 15.0%
!!! TGACTATGAATATTTTAAAA+AGG + chr1_2:8837926-8837945 None:intergenic 15.0%
!! TGTCTAATTTATAAGTTATT+TGG - chr1_2:8838447-8838466 Msa0054160:intron 15.0%
!! GTCTAATTTATAAGTTATTT+GGG - chr1_2:8838448-8838467 Msa0054160:intron 15.0%
!! TCTAATTTATAAGTTATTTG+GGG - chr1_2:8838449-8838468 Msa0054160:intron 15.0%
!! TTTAATGTAATTAATGATTG+CGG + chr1_2:8838596-8838615 None:intergenic 15.0%
!! AACCAATATAAATCAAAATA+CGG - chr1_2:8839087-8839106 Msa0054160:intron 15.0%
!! CTAAAATTATGTTTATAAGT+GGG - chr1_2:8839407-8839426 Msa0054160:intron 15.0%
!! AATGATGATTTACATAAATA+AGG + chr1_2:8839886-8839905 None:intergenic 15.0%
!!! GTTTATTACTTACATTTTCA+GGG + chr1_2:8837355-8837374 None:intergenic 20.0%
!! TTGAAGAAAAATGAAAAAAG+GGG + chr1_2:8837400-8837419 None:intergenic 20.0%
!! CATAACATTACTATCAAATT+TGG + chr1_2:8837808-8837827 None:intergenic 20.0%
!!! CAAATTTGATAGTAATGTTA+TGG - chr1_2:8837806-8837825 Msa0054160:exon 20.0%
!! TTAACCACTTATTATCAATT+TGG + chr1_2:8837865-8837884 None:intergenic 20.0%
!! GATAATAAGTGGTTAAATAT+AGG - chr1_2:8837869-8837888 Msa0054160:intron 20.0%
!!! TTTAAAATATTCATAGTCAC+AGG - chr1_2:8837927-8837946 Msa0054160:intron 20.0%
!! TTAAAATATTCATAGTCACA+GGG - chr1_2:8837928-8837947 Msa0054160:intron 20.0%
!!! AAAGGATCAAAAGATTATTT+TGG + chr1_2:8838394-8838413 None:intergenic 20.0%
!!! ATAGAGTATTGTTTGTTTAA+AGG + chr1_2:8838412-8838431 None:intergenic 20.0%
!! AAAATCTAGCAAAAATACTT+AGG + chr1_2:8838894-8838913 None:intergenic 20.0%
!! CTGAAAATATCTTTATAAGT+GGG - chr1_2:8839206-8839225 Msa0054160:intron 20.0%
!!! CCACTTATAAACATAATTTT+AGG + chr1_2:8839409-8839428 None:intergenic 20.0%
!! CCTAAAATTATGTTTATAAG+TGG - chr1_2:8839406-8839425 Msa0054160:intron 20.0%
!! ATTTATGTAAATCATCATTC+TGG - chr1_2:8839887-8839906 Msa0054160:intron 20.0%
!!! TTGTTATTTATTACTCTAGT+TGG - chr1_2:8840128-8840147 Msa0054160:intron 20.0%
!! GGTTTATTACTTACATTTTC+AGG + chr1_2:8837356-8837375 None:intergenic 25.0%
! TGAAGAAAAATGAAAAAAGG+GGG + chr1_2:8837399-8837418 None:intergenic 25.0%
!! AGAAGAGATTTCAATTTTGA+AGG - chr1_2:8837517-8837536 Msa0054160:three_prime_UTR 25.0%
!! GAAGAGATTTCAATTTTGAA+GGG - chr1_2:8837518-8837537 Msa0054160:three_prime_UTR 25.0%
!!! AAGAGATTTCAATTTTGAAG+GGG - chr1_2:8837519-8837538 Msa0054160:three_prime_UTR 25.0%
!! TAGACCAAATTGATAATAAG+TGG - chr1_2:8837858-8837877 Msa0054160:CDS 25.0%
! ATGCATACATAAATGAGTAA+AGG + chr1_2:8838351-8838370 None:intergenic 25.0%
! CAACATAAGATTATCATTCA+AGG - chr1_2:8838556-8838575 Msa0054160:intron 25.0%
! ATTACATTAAAATGTTGTCG+TGG - chr1_2:8838605-8838624 Msa0054160:intron 25.0%
! TTACATTAAAATGTTGTCGT+GGG - chr1_2:8838606-8838625 Msa0054160:intron 25.0%
! AAAAATACTTAGGAGAGATT+CGG + chr1_2:8838884-8838903 None:intergenic 25.0%
!!! TTGCTAGATTTTGATTTCAT+AGG - chr1_2:8838902-8838921 Msa0054160:intron 25.0%
!!! CTCCGTATTTTGATTTATAT+TGG + chr1_2:8839092-8839111 None:intergenic 25.0%
! TAAGTATTTAACCAAGCTTT+GGG - chr1_2:8839117-8839136 Msa0054160:intron 25.0%
!! CCACTTATAAAGATATTTTC+AGG + chr1_2:8839208-8839227 None:intergenic 25.0%
! CCTGAAAATATCTTTATAAG+TGG - chr1_2:8839205-8839224 Msa0054160:intron 25.0%
! AAATTATGTTTATAAGTGGG+AGG - chr1_2:8839410-8839429 Msa0054160:intron 25.0%
! AGTTGAGAGATACAAAATTT+GGG + chr1_2:8839675-8839694 None:intergenic 25.0%
! AAGTTGAGAGATACAAAATT+TGG + chr1_2:8839676-8839695 None:intergenic 25.0%
! GATCAAATAAGATCAAAAAC+TGG - chr1_2:8839792-8839811 Msa0054160:intron 25.0%
!!! TTTTCTAGCTATTAAAACAG+AGG - chr1_2:8839920-8839939 Msa0054160:intron 25.0%
! AAAGAGATAACAACAGATTA+TGG - chr1_2:8839967-8839986 Msa0054160:intron 25.0%
!! TTTCTAGGAGAGTAAAAAAA+GGG + chr1_2:8840000-8840019 None:intergenic 25.0%
! TTATTTATTACTCTAGTTGG+TGG - chr1_2:8840131-8840150 Msa0054160:intron 25.0%
! ACAACACTTTATACTAAACA+GGG + chr1_2:8840401-8840420 None:intergenic 25.0%
!!! TATAAAGTGTTGTCAAATAG+CGG - chr1_2:8840408-8840427 Msa0054160:intron 25.0%
! TTGTATTACATATTGTATCG+TGG + chr1_2:8840618-8840637 None:intergenic 25.0%
! ACAATATGTAATACAAAGTG+TGG - chr1_2:8840622-8840641 Msa0054160:CDS 25.0%
! TACACTACTAGATTAAAGTT+AGG - chr1_2:8840734-8840753 Msa0054160:intron 25.0%
! GAAATGGTATACAAATGAAA+AGG + chr1_2:8840809-8840828 None:intergenic 25.0%
! TATATATCTGATAAAACCCT+AGG + chr1_2:8841011-8841030 None:intergenic 25.0%
!!! AGGGTTTTATCAGATATATA+CGG - chr1_2:8841011-8841030 Msa0054160:intron 25.0%
! TAAGAAGAATAACCAATACA+CGG + chr1_2:8841069-8841088 None:intergenic 25.0%
!! AAATATGATAGTAGCTGTTT+TGG - chr1_2:8841195-8841214 Msa0054160:intron 25.0%
!!! AATATGATAGTAGCTGTTTT+GGG - chr1_2:8841196-8841215 Msa0054160:intron 25.0%
! ATTTATGAAGTCATAACACT+CGG + chr1_2:8841388-8841407 None:intergenic 25.0%
! ATAAATGTCATATTTACACG+AGG - chr1_2:8841402-8841421 Msa0054160:intron 25.0%
!!! GCATCATTCAAAATTAGTTT+TGG - chr1_2:8841504-8841523 Msa0054160:CDS 25.0%
GTTGAAATTCAGTTTGAAGA+CGG + chr1_2:8837325-8837344 None:intergenic 30.0%
TCTTCAATTCCTGTGATAAT+TGG - chr1_2:8837413-8837432 Msa0054160:three_prime_UTR 30.0%
!! AAAAAGGTACCAATTATCAC+AGG + chr1_2:8837425-8837444 None:intergenic 30.0%
ATCAGTGTTAAGCACAAAAA+AGG + chr1_2:8837441-8837460 None:intergenic 30.0%
TTTGTGCTTAACACTGATAT+TGG - chr1_2:8837443-8837462 Msa0054160:three_prime_UTR 30.0%
TATGACACTCTCAATTCAAA+CGG - chr1_2:8837701-8837720 Msa0054160:three_prime_UTR 30.0%
GGATCAACTTGACAATTTAA+TGG - chr1_2:8837891-8837910 Msa0054160:intron 30.0%
ATTCATATGTTAGTGTGAGA+TGG - chr1_2:8838046-8838065 Msa0054160:intron 30.0%
TTCATATGTTAGTGTGAGAT+GGG - chr1_2:8838047-8838066 Msa0054160:intron 30.0%
ATCATCATCTTCACTAGTAA+CGG + chr1_2:8838199-8838218 None:intergenic 30.0%
GTATGCATAATGATTCATGT+TGG - chr1_2:8838363-8838382 Msa0054160:intron 30.0%
TACATTAAAATGTTGTCGTG+GGG - chr1_2:8838607-8838626 Msa0054160:intron 30.0%
AATTGTGATCACTATGCATT+TGG - chr1_2:8838716-8838735 Msa0054160:intron 30.0%
! TTTGGTGATCTTTGATGTAA+AGG - chr1_2:8838734-8838753 Msa0054160:intron 30.0%
! TTGGTGATCTTTGATGTAAA+GGG - chr1_2:8838735-8838754 Msa0054160:intron 30.0%
AAAAAGCTGAAAACGACTTA+TGG - chr1_2:8838782-8838801 Msa0054160:intron 30.0%
AAAAGCTGAAAACGACTTAT+GGG - chr1_2:8838783-8838802 Msa0054160:intron 30.0%
!!! GAATCTTTTGCTATTCTCAA+AGG - chr1_2:8839013-8839032 Msa0054160:intron 30.0%
! GATTTATATTGGTTGTTTGC+TGG + chr1_2:8839081-8839100 None:intergenic 30.0%
GTAAGTATTTAACCAAGCTT+TGG - chr1_2:8839116-8839135 Msa0054160:intron 30.0%
TATAAGCAAAGCAATGTGAA+AGG + chr1_2:8839145-8839164 None:intergenic 30.0%
TCACATTGCTTTGCTTATAT+AGG - chr1_2:8839146-8839165 Msa0054160:intron 30.0%
CACATTGCTTTGCTTATATA+GGG - chr1_2:8839147-8839166 Msa0054160:intron 30.0%
GGTTATATCTCATCCAATAT+GGG + chr1_2:8839187-8839206 None:intergenic 30.0%
AGGTTATATCTCATCCAATA+TGG + chr1_2:8839188-8839207 None:intergenic 30.0%
AAAATTTAAGCCCTACAAAC+TGG + chr1_2:8839253-8839272 None:intergenic 30.0%
! AAGTCCAACCAAATTTTAAG+AGG - chr1_2:8839475-8839494 Msa0054160:intron 30.0%
AACTAGAGTAATAGCTCAAA+CGG - chr1_2:8839571-8839590 Msa0054160:intron 30.0%
TGAAAACCATTGCAATTACT+TGG + chr1_2:8839731-8839750 None:intergenic 30.0%
!!! ACAATTTTAACTGATTGTGC+AGG - chr1_2:8839752-8839771 Msa0054160:intron 30.0%
TAGCTATTAAAACAGAGGTT+TGG - chr1_2:8839925-8839944 Msa0054160:intron 30.0%
GATAACAACAGATTATGGTT+TGG - chr1_2:8839972-8839991 Msa0054160:intron 30.0%
! TTCTAGGAGAGTAAAAAAAG+GGG + chr1_2:8839999-8840018 None:intergenic 30.0%
! GTTTCTAGGAGAGTAAAAAA+AGG + chr1_2:8840001-8840020 None:intergenic 30.0%
TTAAAAACTATCCTAGAAGC+TGG - chr1_2:8840098-8840117 Msa0054160:intron 30.0%
ACAAAAATATTCCAGCTTCT+AGG + chr1_2:8840112-8840131 None:intergenic 30.0%
AGATGCATTAGTTAGTCATA+AGG - chr1_2:8840226-8840245 Msa0054160:intron 30.0%
TTGTCAAATAGCAGTTATAG+TGG - chr1_2:8840318-8840337 Msa0054160:intron 30.0%
GACAACACTTTATACTAAAC+AGG + chr1_2:8840402-8840421 None:intergenic 30.0%
GAGTGTAATAACAGAATATG+AGG - chr1_2:8840518-8840537 Msa0054160:intron 30.0%
TGGTATACAAATGAAAAGGA+TGG + chr1_2:8840805-8840824 None:intergenic 30.0%
! GCATTTCTGAATTCTAGAAA+TGG + chr1_2:8840825-8840844 None:intergenic 30.0%
!!! CTGTAACATGCATGATTTTT+TGG + chr1_2:8840975-8840994 None:intergenic 30.0%
CTTTCATAGCGAAAACTATT+TGG - chr1_2:8841162-8841181 Msa0054160:intron 30.0%
AAAAATAGCATCAACCATTG+TGG - chr1_2:8841304-8841323 Msa0054160:intron 30.0%
ACATACTGGAAAGTTAATTC+AGG - chr1_2:8841358-8841377 Msa0054160:intron 30.0%
!! TGAAGCTTTTGAGAAGCAAA+TGG - chr1_2:8837220-8837239 Msa0054160:three_prime_UTR 35.0%
!! GAAGCTTTTGAGAAGCAAAT+GGG - chr1_2:8837221-8837240 Msa0054160:three_prime_UTR 35.0%
!! AAGCTTTTGAGAAGCAAATG+GGG - chr1_2:8837222-8837241 Msa0054160:three_prime_UTR 35.0%
TCCTAGAAACAAGCTTCATA+GGG + chr1_2:8837484-8837503 None:intergenic 35.0%
ATCCTAGAAACAAGCTTCAT+AGG + chr1_2:8837485-8837504 None:intergenic 35.0%
CTTTAGATTGAAGACGTGTT+TGG - chr1_2:8837614-8837633 Msa0054160:three_prime_UTR 35.0%
!!! TGTCACTTTTGAGTAAACCA+CGG - chr1_2:8837661-8837680 Msa0054160:three_prime_UTR 35.0%
ATGACACTCTCAATTCAAAC+GGG - chr1_2:8837702-8837721 Msa0054160:three_prime_UTR 35.0%
AAATTAGTCCCTGACATTGT+AGG + chr1_2:8837970-8837989 None:intergenic 35.0%
TACAATGTCAGGGACTAATT+TGG - chr1_2:8837969-8837988 Msa0054160:intron 35.0%
TCATATGTTAGTGTGAGATG+GGG - chr1_2:8838048-8838067 Msa0054160:intron 35.0%
TCATCATCTTCACTAGTAAC+GGG + chr1_2:8838198-8838217 None:intergenic 35.0%
TGCATTCACTAACTTCAAAC+AGG - chr1_2:8838218-8838237 Msa0054160:intron 35.0%
AAGAACACACATTCAGCTAT+AGG + chr1_2:8838293-8838312 None:intergenic 35.0%
TGAAATGCCACACACAAAAA+AGG + chr1_2:8838323-8838342 None:intergenic 35.0%
AGTTGAAAACGCAAAAGAAG+AGG - chr1_2:8838486-8838505 Msa0054160:intron 35.0%
TAGCTATTGTTTCGTCTTTG+TGG - chr1_2:8838527-8838546 Msa0054160:intron 35.0%
! CGAGTTGTTCATTTTCTGAT+TGG - chr1_2:8838659-8838678 Msa0054160:intron 35.0%
! GTTGTTCATTTTCTGATTGG+TGG - chr1_2:8838662-8838681 Msa0054160:intron 35.0%
TGATCTTTGATGTAAAGGGT+TGG - chr1_2:8838739-8838758 Msa0054160:intron 35.0%
AGATGCAGTCTCTTCTATTA+TGG - chr1_2:8838953-8838972 Msa0054160:intron 35.0%
!! TTAAAACACCGCTTTGAAAC+TGG + chr1_2:8839042-8839061 None:intergenic 35.0%
TTGCTTTGCTTATATAGGGA+AGG - chr1_2:8839151-8839170 Msa0054160:intron 35.0%
TGCTTTGCTTATATAGGGAA+GGG - chr1_2:8839152-8839171 Msa0054160:intron 35.0%
ACATTAGAAGCCAGTTTGTA+GGG - chr1_2:8839240-8839259 Msa0054160:intron 35.0%
AAAGCTTATCCAAGATCTGA+CGG - chr1_2:8839284-8839303 Msa0054160:intron 35.0%
AGTAGCTCAAAATTGACAGT+GGG + chr1_2:8839314-8839333 None:intergenic 35.0%
AAGTAGCTCAAAATTGACAG+TGG + chr1_2:8839315-8839334 None:intergenic 35.0%
! ACTGTCAATTTTGAGCTACT+TGG - chr1_2:8839314-8839333 Msa0054160:intron 35.0%
! CTGTCAATTTTGAGCTACTT+GGG - chr1_2:8839315-8839334 Msa0054160:intron 35.0%
TTAATTCAACCCTGCAAAAC+CGG + chr1_2:8839457-8839476 None:intergenic 35.0%
AGGACCTCTTAAAATTTGGT+TGG + chr1_2:8839482-8839501 None:intergenic 35.0%
CAACAGGACCTCTTAAAATT+TGG + chr1_2:8839486-8839505 None:intergenic 35.0%
!! CAAATTTTAAGAGGTCCTGT+TGG - chr1_2:8839484-8839503 Msa0054160:intron 35.0%
ACTAGAGTAATAGCTCAAAC+GGG - chr1_2:8839572-8839591 Msa0054160:intron 35.0%
AAAATCACTTTGCAGATGCT+TGG + chr1_2:8839649-8839668 None:intergenic 35.0%
AGTCAACCAAGTAATTGCAA+TGG - chr1_2:8839722-8839741 Msa0054160:intron 35.0%
! AAAACTGGTGAGCAGTTTTA+CGG - chr1_2:8839807-8839826 Msa0054160:intron 35.0%
TCCTAGAAACTAATCCACTT+GGG - chr1_2:8840011-8840030 Msa0054160:intron 35.0%
TTACACAAACAAAAGCGAAG+AGG + chr1_2:8840191-8840210 None:intergenic 35.0%
TAGTTAGTCATAAGGTCTGT+AGG - chr1_2:8840234-8840253 Msa0054160:intron 35.0%
AAATAGCAGTTATAGTGGCT+CGG - chr1_2:8840323-8840342 Msa0054160:intron 35.0%
TCAAATTCCTCAACACTAGA+GGG + chr1_2:8840450-8840469 None:intergenic 35.0%
TTCAAATTCCTCAACACTAG+AGG + chr1_2:8840451-8840470 None:intergenic 35.0%
GTATGCATGTCATGTTTATC+TGG - chr1_2:8840550-8840569 Msa0054160:CDS 35.0%
TACAAAGTGTGGTCAAATAG+TGG - chr1_2:8840633-8840652 Msa0054160:intron 35.0%
! TGTACCAGTGTTGTTAATCA+CGG + chr1_2:8840718-8840737 None:intergenic 35.0%
!!! CTGTAAATGTGTTTTGATGC+AGG - chr1_2:8840860-8840879 Msa0054160:intron 35.0%
!!! TGCATGATTTTTTGGTTGTC+TGG + chr1_2:8840967-8840986 None:intergenic 35.0%
!!! ACAGTTTTAGTTCAGTTCCT+AGG - chr1_2:8840991-8841010 Msa0054160:intron 35.0%
!!! CAGTTTTAGTTCAGTTCCTA+GGG - chr1_2:8840992-8841011 Msa0054160:intron 35.0%
AATAGAACTTTGCCGTGTAT+TGG - chr1_2:8841054-8841073 Msa0054160:intron 35.0%
GCTACAATATGTAGCTTCAA+TGG + chr1_2:8841143-8841162 None:intergenic 35.0%
CCAGTATGTTACACATGTTA+TGG + chr1_2:8841347-8841366 None:intergenic 35.0%
CCATAACATGTGTAACATAC+TGG - chr1_2:8841344-8841363 Msa0054160:intron 35.0%
ATGTCATATTTACACGAGGA+AGG - chr1_2:8841406-8841425 Msa0054160:CDS 35.0%
GAATACAATGAAGCAACCGA+CGG - chr1_2:8837248-8837267 Msa0054160:three_prime_UTR 40.0%
GAATAGTTTCACAAGTCCGT+CGG + chr1_2:8837267-8837286 None:intergenic 40.0%
! GTGAAAAGTGTAATATGGGG+GGG + chr1_2:8837377-8837396 None:intergenic 40.0%
! GGTGAAAAGTGTAATATGGG+GGG + chr1_2:8837378-8837397 None:intergenic 40.0%
! GGGTGAAAAGTGTAATATGG+GGG + chr1_2:8837379-8837398 None:intergenic 40.0%
! GGGGTGAAAAGTGTAATATG+GGG + chr1_2:8837380-8837399 None:intergenic 40.0%
! GGGGGTGAAAAGTGTAATAT+GGG + chr1_2:8837381-8837400 None:intergenic 40.0%
! AGGGGGTGAAAAGTGTAATA+TGG + chr1_2:8837382-8837401 None:intergenic 40.0%
!!! CACTGATATTGGTTTTCTGC+AGG - chr1_2:8837454-8837473 Msa0054160:three_prime_UTR 40.0%
! ACCCTATGAAGCTTGTTTCT+AGG - chr1_2:8837480-8837499 Msa0054160:three_prime_UTR 40.0%
AATGTCAGGGACTAATTTGG+TGG - chr1_2:8837972-8837991 Msa0054160:intron 40.0%
CATCATCTTCACTAGTAACG+GGG + chr1_2:8838197-8838216 None:intergenic 40.0%
!!! TGATGTGCCTTTTTTGTGTG+TGG - chr1_2:8838313-8838332 Msa0054160:intron 40.0%
TGAAAACGCAAAAGAAGAGG+TGG - chr1_2:8838489-8838508 Msa0054160:intron 40.0%
GAAAACGCAAAAGAAGAGGT+GGG - chr1_2:8838490-8838509 Msa0054160:intron 40.0%
TTAAAATGTTGTCGTGGGGA+TGG - chr1_2:8838611-8838630 Msa0054160:intron 40.0%
GGATGGTTTATTGGTAGAGA+AGG - chr1_2:8838628-8838647 Msa0054160:intron 40.0%
GGTTTATTGGTAGAGAAGGA+AGG - chr1_2:8838632-8838651 Msa0054160:intron 40.0%
AATAGCCAAGTGAAGAGTGA+TGG - chr1_2:8838978-8838997 Msa0054160:intron 40.0%
AGTTACCATCACTCTTCACT+TGG + chr1_2:8838986-8839005 None:intergenic 40.0%
AAAGGTTGCCAGTTTCAAAG+CGG - chr1_2:8839031-8839050 Msa0054160:intron 40.0%
GCTTTGCTTATATAGGGAAG+GGG - chr1_2:8839153-8839172 Msa0054160:intron 40.0%
ATCTCATCCAATATGGGAAC+AGG + chr1_2:8839181-8839200 None:intergenic 40.0%
! CAAACTGGCTTCTAATGTGA+GGG + chr1_2:8839238-8839257 None:intergenic 40.0%
! ACAAACTGGCTTCTAATGTG+AGG + chr1_2:8839239-8839258 None:intergenic 40.0%
CACATTAGAAGCCAGTTTGT+AGG - chr1_2:8839239-8839258 Msa0054160:intron 40.0%
AAGCTTATCCAAGATCTGAC+GGG - chr1_2:8839285-8839304 Msa0054160:intron 40.0%
AAGACTGAACATTTGCAGCA+TGG + chr1_2:8839341-8839360 None:intergenic 40.0%
TGCTGCAAATGTTCAGTCTT+AGG - chr1_2:8839341-8839360 Msa0054160:intron 40.0%
GTTCCTCATTGGATGACATA+TGG - chr1_2:8839383-8839402 Msa0054160:intron 40.0%
!!! AGGTCCTGTTGGTTTTTAGT+TGG - chr1_2:8839495-8839514 Msa0054160:intron 40.0%
TCTGCCAACTAAAAACCAAC+AGG + chr1_2:8839502-8839521 None:intergenic 40.0%
!!! GTTTTTAGTTGGCAGAACGT+GGG - chr1_2:8839506-8839525 Msa0054160:intron 40.0%
AGGGAGAAGGTAAAATACAG+AGG - chr1_2:8839530-8839549 Msa0054160:intron 40.0%
TTGTGCAGGTGATTGATGAA+TGG - chr1_2:8839766-8839785 Msa0054160:intron 40.0%
! GAGCAGTTTTACGGACTTTT+TGG - chr1_2:8839816-8839835 Msa0054160:intron 40.0%
TCAAATGTACAGCATGTGCA+TGG + chr1_2:8839854-8839873 None:intergenic 40.0%
CTCCTAGAAACTAATCCACT+TGG - chr1_2:8840010-8840029 Msa0054160:intron 40.0%
!! ACCCAAGTGGATTAGTTTCT+AGG + chr1_2:8840015-8840034 None:intergenic 40.0%
AGAAACTAATCCACTTGGGT+TGG - chr1_2:8840015-8840034 Msa0054160:intron 40.0%
CAAAAGCGAAGAGGAGAAAA+AGG + chr1_2:8840182-8840201 None:intergenic 40.0%
TCGATTCCTTCAATGAAGAG+AGG - chr1_2:8840269-8840288 Msa0054160:intron 40.0%
TTATACTAAACAGGGTGACG+CGG + chr1_2:8840393-8840412 None:intergenic 40.0%
! ACATCAAGTGTTGTCAATCG+TGG + chr1_2:8840495-8840514 None:intergenic 40.0%
TGCATGTCATGTTTATCTGG+CGG - chr1_2:8840553-8840572 Msa0054160:CDS 40.0%
GAACCACAAGATTCTGATTC+TGG - chr1_2:8840887-8840906 Msa0054160:intron 40.0%
TCTCCAGAATCAGAATCTTG+TGG + chr1_2:8840893-8840912 None:intergenic 40.0%
! TAATTTGTGTGCTTCTGCTG+TGG - chr1_2:8841224-8841243 Msa0054160:intron 40.0%
TTCTATCTTGCTCACCACAA+TGG + chr1_2:8841321-8841340 None:intergenic 40.0%
TTGTGAAGGAATGACATGAG+TGG - chr1_2:8841464-8841483 Msa0054160:CDS 40.0%
GTAAACCACGGAATTCGACA+TGG - chr1_2:8837673-8837692 Msa0054160:three_prime_UTR 45.0%
GAAAACCATGTCGAATTCCG+TGG + chr1_2:8837681-8837700 None:intergenic 45.0%
GACAGAGTCAGTGACATTTG+TGG + chr1_2:8837748-8837767 None:intergenic 45.0%
! TATCAGTCCTTCTGAGAGTG+CGG - chr1_2:8837780-8837799 Msa0054160:three_prime_UTR 45.0%
ATTGTAGGCCACTGTCGAAT+AGG + chr1_2:8837955-8837974 None:intergenic 45.0%
GTGAGATGGGGATAAGATCT+AGG - chr1_2:8838060-8838079 Msa0054160:intron 45.0%
CAGCGTATGCAATCATCTGT+GGG - chr1_2:8838168-8838187 Msa0054160:intron 45.0%
AAGAGGTGGGACATGTCATA+CGG - chr1_2:8838503-8838522 Msa0054160:intron 45.0%
TTGTCGTGGGGATGGTTTAT+TGG - chr1_2:8838619-8838638 Msa0054160:intron 45.0%
! TTGGTTGTTTGCTGGCAAGA+GGG + chr1_2:8839073-8839092 None:intergenic 45.0%
! ATTGGTTGTTTGCTGGCAAG+AGG + chr1_2:8839074-8839093 None:intergenic 45.0%
!! TGTGAAAGGTACCCAAAGCT+TGG + chr1_2:8839131-8839150 None:intergenic 45.0%
AATGTTCAGTCTTAGGCGTG+AGG - chr1_2:8839348-8839367 Msa0054160:intron 45.0%
ATGTTCAGTCTTAGGCGTGA+GGG - chr1_2:8839349-8839368 Msa0054160:intron 45.0%
AGGCCATATGTCATCCAATG+AGG + chr1_2:8839389-8839408 None:intergenic 45.0%
!!! GGTTTTTAGTTGGCAGAACG+TGG - chr1_2:8839505-8839524 Msa0054160:intron 45.0%
ACAGCATGTGCATGGACAAT+TGG + chr1_2:8839846-8839865 None:intergenic 45.0%
!! TAAGGTCTGTAGGAGTGCTT+AGG - chr1_2:8840244-8840263 Msa0054160:intron 45.0%
!! AAGGTCTGTAGGAGTGCTTA+GGG - chr1_2:8840245-8840264 Msa0054160:intron 45.0%
CACTCTCCTCTCTTCATTGA+AGG + chr1_2:8840278-8840297 None:intergenic 45.0%
CTTCAATGAAGAGAGGAGAG+TGG - chr1_2:8840276-8840295 Msa0054160:intron 45.0%
TAGTGGCTATAGTGCTGTAG+CGG - chr1_2:8840650-8840669 Msa0054160:intron 45.0%
! CCAGTGTTGTTAATCACGGA+TGG + chr1_2:8840714-8840733 None:intergenic 45.0%
CCATCCGTGATTAACAACAC+TGG - chr1_2:8840711-8840730 Msa0054160:intron 45.0%
GTGAGTGTTTCGATCTGTGT+TGG - chr1_2:8841099-8841118 Msa0054160:intron 45.0%
GTGTGCACACAGAAACAGTA+TGG - chr1_2:8841274-8841293 Msa0054160:intron 45.0%
GCACACAGAAACAGTATGGT+AGG - chr1_2:8841278-8841297 Msa0054160:intron 45.0%
! CTGCAAGCTGCATTTTGTGA+AGG - chr1_2:8841450-8841469 Msa0054160:CDS 45.0%
TGAGCTTCTTGCTGCTAAGC+AGG - chr1_2:8837550-8837569 Msa0054160:three_prime_UTR 50.0%
! ATCAGTCCTTCTGAGAGTGC+GGG - chr1_2:8837781-8837800 Msa0054160:three_prime_UTR 50.0%
GACAGTGGCCTACAATGTCA+GGG - chr1_2:8837959-8837978 Msa0054160:intron 50.0%
! GTTTGCTTGTTGAGCTGTGC+AGG - chr1_2:8838095-8838114 Msa0054160:intron 50.0%
GCAGCGTATGCAATCATCTG+TGG - chr1_2:8838167-8838186 Msa0054160:intron 50.0%
AGGGGAACCTGTTCCCATAT+TGG - chr1_2:8839171-8839190 Msa0054160:intron 50.0%
TGTTCAGTCTTAGGCGTGAG+GGG - chr1_2:8839350-8839369 Msa0054160:intron 50.0%
GTGCGTTGAGTGTTCCTCAT+TGG - chr1_2:8839372-8839391 Msa0054160:intron 50.0%
TGCAAAACCGGCAGCTTGTA+AGG + chr1_2:8839445-8839464 None:intergenic 50.0%
!!! TTACAAGCTGCCGGTTTTGC+AGG - chr1_2:8839444-8839463 Msa0054160:intron 50.0%
!!! TACAAGCTGCCGGTTTTGCA+GGG - chr1_2:8839445-8839464 Msa0054160:intron 50.0%
TTAGTTGGCAGAACGTGGGA+AGG - chr1_2:8839510-8839529 Msa0054160:intron 50.0%
TAGTTGGCAGAACGTGGGAA+GGG - chr1_2:8839511-8839530 Msa0054160:intron 50.0%
GATCTCAAGCCAACACCACT+AGG + chr1_2:8840041-8840060 None:intergenic 50.0%
!! GTGGTGTTGGCTTGAGATCT+TGG - chr1_2:8840042-8840061 Msa0054160:intron 50.0%
CTTGGAGTGTGCTCTTCTCA+AGG - chr1_2:8840060-8840079 Msa0054160:intron 50.0%
TGCTCAACCCTCTAGTGTTG+AGG - chr1_2:8840440-8840459 Msa0054160:intron 50.0%
GTGTTGTCAATCGTGGAACG+TGG + chr1_2:8840488-8840507 None:intergenic 50.0%
GCAGCGAAATGAAGACAGCA+TGG + chr1_2:8840772-8840791 None:intergenic 50.0%
GCTGTCTTCATTTCGCTGCA+TGG - chr1_2:8840773-8840792 Msa0054160:intron 50.0%
TTGTGTGCTTCTGCTGTGGA+AGG - chr1_2:8841228-8841247 Msa0054160:intron 50.0%
TGTGTGCTTCTGCTGTGGAA+GGG - chr1_2:8841229-8841248 Msa0054160:intron 50.0%
TTTGGTCCCGCACTCTCAGA+AGG + chr1_2:8837790-8837809 None:intergenic 55.0%
CACAGGGACCTATTCGACAG+TGG - chr1_2:8837944-8837963 Msa0054160:intron 55.0%
CGACAGTGGCCTACAATGTC+AGG - chr1_2:8837958-8837977 Msa0054160:intron 55.0%
TTCCTCGCCTTACAAGCTGC+CGG - chr1_2:8839435-8839454 Msa0054160:intron 55.0%
!! ATCCACTTGGGTTGGCCTAG+TGG - chr1_2:8840023-8840042 Msa0054160:intron 55.0%
!! TTGGGTTGGCCTAGTGGTGT+TGG - chr1_2:8840029-8840048 Msa0054160:intron 55.0%
! ATAGTGGCTCGGCAGTGTTG+TGG - chr1_2:8840334-8840353 Msa0054160:intron 55.0%
! GTGGGTTGCCCGTCAGATCT+TGG + chr1_2:8839296-8839315 None:intergenic 60.0%
AACCGGCAGCTTGTAAGGCG+AGG + chr1_2:8839440-8839459 None:intergenic 60.0%
GCAGAACGTGGGAAGGGAGA+AGG - chr1_2:8839517-8839536 Msa0054160:intron 60.0%
CACCACTAGGCCAACCCAAG+TGG + chr1_2:8840028-8840047 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 8837212 8841555 8837212 ID=Msa0054160;Name=Msa0054160
chr1_2 mRNA 8837212 8841555 8837212 ID=Msa0054160-mRNA-1;Parent=Msa0054160;Name=Msa0054160-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.66|0.75|1|0.33|0.75|4|600|128
chr1_2 exon 8837212 8837885 8837212 ID=Msa0054160-mRNA-1:exon:3183;Parent=Msa0054160-mRNA-1
chr1_2 exon 8838954 8838993 8838954 ID=Msa0054160-mRNA-1:exon:3182;Parent=Msa0054160-mRNA-1
chr1_2 exon 8840528 8840650 8840528 ID=Msa0054160-mRNA-1:exon:3181;Parent=Msa0054160-mRNA-1
chr1_2 exon 8841406 8841555 8841406 ID=Msa0054160-mRNA-1:exon:3180;Parent=Msa0054160-mRNA-1
chr1_2 CDS 8841406 8841555 8841406 ID=Msa0054160-mRNA-1:cds;Parent=Msa0054160-mRNA-1
chr1_2 CDS 8840528 8840650 8840528 ID=Msa0054160-mRNA-1:cds;Parent=Msa0054160-mRNA-1
chr1_2 CDS 8838954 8838993 8838954 ID=Msa0054160-mRNA-1:cds;Parent=Msa0054160-mRNA-1
chr1_2 CDS 8837812 8837885 8837812 ID=Msa0054160-mRNA-1:cds;Parent=Msa0054160-mRNA-1
chr1_2 three_prime_UTR 8837212 8837811 8837212 ID=Msa0054160-mRNA-1:three_prime_utr;Parent=Msa0054160-mRNA-1
Gene Sequence

>Msa0054160

TGGAAGTTTGAAGCTTTTGAGAAGCAAATGGGGAATGAATACAATGAAGCAACCGACGGACTTGTGAAACTATTCAGAAAAGCAAATCACGATCTTGATATCGTTCAACACCGTCTTCAAACTGAATTTCAACAGCAGTACCCTGAAAATGATTTGATTGATAAAGCTCAGACAGTTCTTGTTGAGAATAGAAATTTAGTGCAGCGTATGCAATCATCTGTGGGAATCCCCGTTACTAGTGAAGATGATGATGCATTCACTAACTTCAAACAGGTGATTGATGAATGGACTGATCAAATAAGATCAAAAACTGGAAATGAACCACAAGATTCTGATTCTGGAGATCTCAACAAACTACTCTTTTCAGCTATAGTTCAAAGTAATTGA
Protein sequence

>Msa0054160

WKFEAFEKQMGNEYNEATDGLVKLFRKANHDLDIVQHRLQTEFQQQYPENDLIDKAQTVLVENRNLVQRMQSSVGIPVTSEDDDAFTNFKQVIDEWTDQIRSKTGNEPQDSDSGDLNKLLFSAIVQSN*