AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0099770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099770 MtrunA17_Chr1g0150371 77.483 151 2 1 1 119 1 151 8.26e-78 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099770 AT2G24970.1 40.940 149 56 1 3 119 4 152 3.63e-35 118

Find 33 sgRNAs with CRISPR-Local

Find 281 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATATGATAGTAGCTGTTT+TGG 0.249235 1_3:+6045595 Msa0099770:three_prime_UTR
CTTTCATAGCGAAAACTATT+TGG 0.250674 1_3:+6045562 Msa0099770:three_prime_UTR
GAACCACAAGATTCTGATTC+TGG 0.302148 1_3:+6045287 Msa0099770:CDS
GCATCATTCAAAATTAGTTT+TGG 0.306642 1_3:+6045904 Msa0099770:three_prime_UTR
CCAGTATGTTACACATGTTA+TGG 0.354700 1_3:-6045744 None:intergenic
CCATAACATGTGTAACATAC+TGG 0.388934 1_3:+6045744 Msa0099770:three_prime_UTR
AATAGAACTTTGCCGTGTAT+TGG 0.392603 1_3:+6045454 Msa0099770:three_prime_UTR
ACATACTGGAAAGTTAATTC+AGG 0.415415 1_3:+6045758 Msa0099770:three_prime_UTR
TCATCATCTTCACTAGTAAC+GGG 0.420623 1_3:-6042595 None:intergenic
TGCATTCACTAACTTCAAAC+AGG 0.459588 1_3:+6042618 Msa0099770:CDS
GCTACAATATGTAGCTTCAA+TGG 0.460763 1_3:-6045540 None:intergenic
TTGTGTGCTTCTGCTGTGGA+AGG 0.501500 1_3:+6045628 Msa0099770:three_prime_UTR
GTGTGCACACAGAAACAGTA+TGG 0.501688 1_3:+6045674 Msa0099770:three_prime_UTR
ATCATCATCTTCACTAGTAA+CGG 0.530991 1_3:-6042596 None:intergenic
GTGAGTGTTTCGATCTGTGT+TGG 0.548936 1_3:+6045499 Msa0099770:three_prime_UTR
TAACTTGTGTGCTTCTGCTG+TGG 0.553563 1_3:+6045624 Msa0099770:three_prime_UTR
TTCTATCTTGCTCACCACAA+TGG 0.578942 1_3:-6045718 None:intergenic
TATATATCTGATAAAACCCT+AGG 0.583849 1_3:-6045408 None:intergenic
ATGTCATATTTACACGAGGA+AGG 0.586125 1_3:+6045806 Msa0099770:three_prime_UTR
GCAGCGTATGCAATCATCTG+TGG 0.599854 1_3:+6042567 Msa0099770:CDS
TTGTGCAGGTGATTGATGAA+TGG 0.600689 1_3:+6044166 Msa0099770:intron
CAGCGTATGCAATCATCTGT+GGG 0.606247 1_3:+6042568 Msa0099770:CDS
GCACACAGAAACAGTATGGT+AGG 0.608143 1_3:+6045678 Msa0099770:three_prime_UTR
GAATAGTTTCACAAGTCCGT+CGG 0.620054 1_3:-6041663 None:intergenic
GTTGAAATTCAGTTTGAAGA+CGG 0.620867 1_3:-6041721 None:intergenic
ATTTATGAAGTCATAACACT+CGG 0.635098 1_3:-6045785 None:intergenic
TGTGTGCTTCTGCTGTGGAA+GGG 0.640233 1_3:+6045629 Msa0099770:three_prime_UTR
TCTCCAGAATCAGAATCTTG+TGG 0.645712 1_3:-6045290 None:intergenic
CATCATCTTCACTAGTAACG+GGG 0.657093 1_3:-6042594 None:intergenic
TTGTGAAGGAATGACATGAG+TGG 0.677030 1_3:+6045864 Msa0099770:three_prime_UTR
GAATACAATGAAGCAACCGA+CGG 0.695465 1_3:+6041647 Msa0099770:CDS
TAAGAAGAATAACCAATACA+CGG 0.712508 1_3:-6045466 None:intergenic
ATAAATGTCATATTTACACG+AGG 0.735678 1_3:+6045802 Msa0099770:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAAGAAAAATGAAAAAA+GGG - chr1_3:6041801-6041820 None:intergenic 15.0%
!! AATTGAAGAAAAATGAAAAA+AGG - chr1_3:6041802-6041821 None:intergenic 15.0%
!! ATAATAAGTGGTTAAATATA+GGG + chr1_3:6042270-6042289 Msa0099770:intron 15.0%
!!! TGACTATGAATATTTTAAAA+AGG - chr1_3:6042326-6042345 None:intergenic 15.0%
!! TGTCTAATTTATAAGTTATT+TGG + chr1_3:6042847-6042866 Msa0099770:intron 15.0%
!! GTCTAATTTATAAGTTATTT+GGG + chr1_3:6042848-6042867 Msa0099770:intron 15.0%
!! TCTAATTTATAAGTTATTTG+GGG + chr1_3:6042849-6042868 Msa0099770:intron 15.0%
!! TTTAATGTAATTAATGATTG+CGG - chr1_3:6042996-6043015 None:intergenic 15.0%
!! AACCAATATAAATCAAAATA+CGG + chr1_3:6043487-6043506 Msa0099770:intron 15.0%
!! CTAAAATTATGTTTATAAGT+GGG + chr1_3:6043807-6043826 Msa0099770:intron 15.0%
!! AATGATGATTTACATAAATA+AGG - chr1_3:6044286-6044305 None:intergenic 15.0%
!!! GTTTATTACTTACATTTTCA+GGG - chr1_3:6041754-6041773 None:intergenic 20.0%
!! TTGAAGAAAAATGAAAAAAG+GGG - chr1_3:6041800-6041819 None:intergenic 20.0%
!! CATAACATTACTATCAAATT+TGG - chr1_3:6042208-6042227 None:intergenic 20.0%
!!! CAAATTTGATAGTAATGTTA+TGG + chr1_3:6042206-6042225 Msa0099770:intron 20.0%
!! TTAACCACTTATTATCAATT+TGG - chr1_3:6042265-6042284 None:intergenic 20.0%
!! GATAATAAGTGGTTAAATAT+AGG + chr1_3:6042269-6042288 Msa0099770:intron 20.0%
!!! TTTAAAATATTCATAGTCAC+AGG + chr1_3:6042327-6042346 Msa0099770:intron 20.0%
!! TTAAAATATTCATAGTCACA+GGG + chr1_3:6042328-6042347 Msa0099770:intron 20.0%
!!! AAAGGATCAAAAGATTATTT+TGG - chr1_3:6042794-6042813 None:intergenic 20.0%
!!! ATAGAGTTTTGTTTGTTTAA+AGG - chr1_3:6042812-6042831 None:intergenic 20.0%
!! AAAATCTAGCAAAAATACTT+AGG - chr1_3:6043294-6043313 None:intergenic 20.0%
!! CTGAAAATATCTTTATAAGT+GGG + chr1_3:6043606-6043625 Msa0099770:intron 20.0%
!!! CCACTTATAAACATAATTTT+AGG - chr1_3:6043809-6043828 None:intergenic 20.0%
!! CCTAAAATTATGTTTATAAG+TGG + chr1_3:6043806-6043825 Msa0099770:intron 20.0%
!! ATTTATGTAAATCATCATTC+TGG + chr1_3:6044287-6044306 Msa0099770:intron 20.0%
!!! TTGTTATTTATTACTCTAGT+TGG + chr1_3:6044528-6044547 Msa0099770:intron 20.0%
!! GGTTTATTACTTACATTTTC+AGG - chr1_3:6041755-6041774 None:intergenic 25.0%
! TGAAGAAAAATGAAAAAAGG+GGG - chr1_3:6041799-6041818 None:intergenic 25.0%
!! AGAAGAGATTTCAATTTTGA+AGG + chr1_3:6041917-6041936 Msa0099770:intron 25.0%
!! GAAGAGATTTCAATTTTGAA+GGG + chr1_3:6041918-6041937 Msa0099770:intron 25.0%
!!! AAGAGATTTCAATTTTGAAG+GGG + chr1_3:6041919-6041938 Msa0099770:intron 25.0%
!! TAGACCAAATTGATAATAAG+TGG + chr1_3:6042258-6042277 Msa0099770:intron 25.0%
! ATGCATACATAAATGAGTAA+AGG - chr1_3:6042751-6042770 None:intergenic 25.0%
! ATTACATTAAAATGTTGTCG+TGG + chr1_3:6043005-6043024 Msa0099770:intron 25.0%
! TTACATTAAAATGTTGTCGT+GGG + chr1_3:6043006-6043025 Msa0099770:intron 25.0%
! AAAAATACTTAGGAGAGATT+CGG - chr1_3:6043284-6043303 None:intergenic 25.0%
!!! TTGCTAGATTTTGATTTCAT+AGG + chr1_3:6043302-6043321 Msa0099770:intron 25.0%
!!! CTCCGTATTTTGATTTATAT+TGG - chr1_3:6043492-6043511 None:intergenic 25.0%
! TAAGTATTTAACCAAGCTTT+GGG + chr1_3:6043517-6043536 Msa0099770:intron 25.0%
!! CCACTTATAAAGATATTTTC+AGG - chr1_3:6043608-6043627 None:intergenic 25.0%
! CCTGAAAATATCTTTATAAG+TGG + chr1_3:6043605-6043624 Msa0099770:intron 25.0%
! AAATTATGTTTATAAGTGGG+AGG + chr1_3:6043810-6043829 Msa0099770:intron 25.0%
! AGTTGAGAGATACAAAATTT+GGG - chr1_3:6044075-6044094 None:intergenic 25.0%
! AAGTTGAGAGATACAAAATT+TGG - chr1_3:6044076-6044095 None:intergenic 25.0%
! GATCAAATAAGATCAAAAAC+TGG + chr1_3:6044192-6044211 Msa0099770:CDS 25.0%
!!! TTTTCTAGCTATTAAAACAG+AGG + chr1_3:6044320-6044339 Msa0099770:intron 25.0%
! AAAGAGATAACAACAGATTA+TGG + chr1_3:6044367-6044386 Msa0099770:intron 25.0%
!! TTTCTAGGAGAGTAAAAAAA+GGG - chr1_3:6044400-6044419 None:intergenic 25.0%
! TTATTTATTACTCTAGTTGG+TGG + chr1_3:6044531-6044550 Msa0099770:intron 25.0%
! ACAACACTTTATACTAAACA+GGG - chr1_3:6044801-6044820 None:intergenic 25.0%
!!! TATAAAGTGTTGTCAAATAG+CGG + chr1_3:6044808-6044827 Msa0099770:intron 25.0%
! TTGTATTACATATTGTATCG+TGG - chr1_3:6045018-6045037 None:intergenic 25.0%
! ACAATATGTAATACAAAGTG+TGG + chr1_3:6045022-6045041 Msa0099770:intron 25.0%
! TACACTACTAGATTAAAGTT+AGG + chr1_3:6045134-6045153 Msa0099770:intron 25.0%
! GAAATGGTATACAAATGAAA+AGG - chr1_3:6045209-6045228 None:intergenic 25.0%
! TATATATCTGATAAAACCCT+AGG - chr1_3:6045411-6045430 None:intergenic 25.0%
!!! AGGGTTTTATCAGATATATA+CGG + chr1_3:6045411-6045430 Msa0099770:three_prime_UTR 25.0%
! TAAGAAGAATAACCAATACA+CGG - chr1_3:6045469-6045488 None:intergenic 25.0%
!! AAATATGATAGTAGCTGTTT+TGG + chr1_3:6045595-6045614 Msa0099770:three_prime_UTR 25.0%
!!! AATATGATAGTAGCTGTTTT+GGG + chr1_3:6045596-6045615 Msa0099770:three_prime_UTR 25.0%
! ATTTATGAAGTCATAACACT+CGG - chr1_3:6045788-6045807 None:intergenic 25.0%
! ATAAATGTCATATTTACACG+AGG + chr1_3:6045802-6045821 Msa0099770:three_prime_UTR 25.0%
!!! GCATCATTCAAAATTAGTTT+TGG + chr1_3:6045904-6045923 Msa0099770:three_prime_UTR 25.0%
GTTGAAATTCAGTTTGAAGA+CGG - chr1_3:6041724-6041743 None:intergenic 30.0%
TCTTCAATTCCTGTGATAAT+TGG + chr1_3:6041813-6041832 Msa0099770:intron 30.0%
!! AAAAAGGTACCAATTATCAC+AGG - chr1_3:6041825-6041844 None:intergenic 30.0%
ATCAGTGTTAAGCACAAAAA+AGG - chr1_3:6041841-6041860 None:intergenic 30.0%
TTTGTGCTTAACACTGATAT+TGG + chr1_3:6041843-6041862 Msa0099770:intron 30.0%
AAACACGTCTTCAATCTAAA+GGG - chr1_3:6042015-6042034 None:intergenic 30.0%
ATAGAAAACCATGTCGAATT+CGG - chr1_3:6042084-6042103 None:intergenic 30.0%
TATGACACTCTCAATTCAAA+CGG + chr1_3:6042101-6042120 Msa0099770:intron 30.0%
GGATCAACTTGACAATTTAA+TGG + chr1_3:6042291-6042310 Msa0099770:intron 30.0%
ATTCATATGTTAGTGTGAGA+TGG + chr1_3:6042446-6042465 Msa0099770:intron 30.0%
TTCATATGTTAGTGTGAGAT+GGG + chr1_3:6042447-6042466 Msa0099770:intron 30.0%
ATCATCATCTTCACTAGTAA+CGG - chr1_3:6042599-6042618 None:intergenic 30.0%
GTATGCATAATGATTCATGT+TGG + chr1_3:6042763-6042782 Msa0099770:intron 30.0%
CAACATAAGATTATCGTTCA+AGG + chr1_3:6042956-6042975 Msa0099770:intron 30.0%
TACATTAAAATGTTGTCGTG+GGG + chr1_3:6043007-6043026 Msa0099770:intron 30.0%
AATTGTGATCACTATGCATT+TGG + chr1_3:6043116-6043135 Msa0099770:intron 30.0%
! TTTGGTGATCTTTGATGTAA+AGG + chr1_3:6043134-6043153 Msa0099770:intron 30.0%
! TTGGTGATCTTTGATGTAAA+GGG + chr1_3:6043135-6043154 Msa0099770:intron 30.0%
AAAAAGCTGAAAACGACTTA+TGG + chr1_3:6043182-6043201 Msa0099770:intron 30.0%
AAAAGCTGAAAACGACTTAT+GGG + chr1_3:6043183-6043202 Msa0099770:intron 30.0%
!!! GAATCTTTTGCTATTCTCAA+AGG + chr1_3:6043413-6043432 Msa0099770:intron 30.0%
! GATTTATATTGGTTGTTTGC+TGG - chr1_3:6043481-6043500 None:intergenic 30.0%
GTAAGTATTTAACCAAGCTT+TGG + chr1_3:6043516-6043535 Msa0099770:intron 30.0%
TATAAGCAAAGCAATGTGAA+AGG - chr1_3:6043545-6043564 None:intergenic 30.0%
TCACATTGCTTTGCTTATAT+AGG + chr1_3:6043546-6043565 Msa0099770:intron 30.0%
CACATTGCTTTGCTTATATA+GGG + chr1_3:6043547-6043566 Msa0099770:intron 30.0%
GGTTATATCTCATCCAATAT+GGG - chr1_3:6043587-6043606 None:intergenic 30.0%
AGGTTATATCTCATCCAATA+TGG - chr1_3:6043588-6043607 None:intergenic 30.0%
AAAATTTAAGCCCTACAAAC+TGG - chr1_3:6043653-6043672 None:intergenic 30.0%
! AAGTCCAACCAAATTTTAAG+AGG + chr1_3:6043875-6043894 Msa0099770:intron 30.0%
AACTAGAGTAATAGCTCAAA+CGG + chr1_3:6043971-6043990 Msa0099770:intron 30.0%
TGAAAACCATTGCAATTACT+TGG - chr1_3:6044131-6044150 None:intergenic 30.0%
!!! ACAATTTTAACTGATTGTGC+AGG + chr1_3:6044152-6044171 Msa0099770:intron 30.0%
TAGCTATTAAAACAGAGGTT+TGG + chr1_3:6044325-6044344 Msa0099770:intron 30.0%
GATAACAACAGATTATGGTT+TGG + chr1_3:6044372-6044391 Msa0099770:intron 30.0%
! TTCTAGGAGAGTAAAAAAAG+GGG - chr1_3:6044399-6044418 None:intergenic 30.0%
! GTTTCTAGGAGAGTAAAAAA+AGG - chr1_3:6044401-6044420 None:intergenic 30.0%
TTAAAAACTATCCTAGAAGC+TGG + chr1_3:6044498-6044517 Msa0099770:intron 30.0%
ACAAAAATATTCCAGCTTCT+AGG - chr1_3:6044512-6044531 None:intergenic 30.0%
AGATGCATTAGTTAGTCATA+AGG + chr1_3:6044626-6044645 Msa0099770:intron 30.0%
TTGTCAAATAGCAGTTATAG+TGG + chr1_3:6044718-6044737 Msa0099770:intron 30.0%
GACAACACTTTATACTAAAC+AGG - chr1_3:6044802-6044821 None:intergenic 30.0%
GAGTGTAATAACAGAATATG+AGG + chr1_3:6044918-6044937 Msa0099770:intron 30.0%
TGGTATACAAATGAAAAGGA+TGG - chr1_3:6045205-6045224 None:intergenic 30.0%
! GCATTTCTGAATTCTAGAAA+TGG - chr1_3:6045225-6045244 None:intergenic 30.0%
!!! CTGTAACATGCATGATTTTT+TGG - chr1_3:6045375-6045394 None:intergenic 30.0%
CTTTCATAGCGAAAACTATT+TGG + chr1_3:6045562-6045581 Msa0099770:three_prime_UTR 30.0%
AAAAATAGCATCAACCATTG+TGG + chr1_3:6045704-6045723 Msa0099770:three_prime_UTR 30.0%
ACATACTGGAAAGTTAATTC+AGG + chr1_3:6045758-6045777 Msa0099770:three_prime_UTR 30.0%
!! TGAAGCTTTTGAGAAGCAAA+TGG + chr1_3:6041619-6041638 Msa0099770:exon 35.0%
!! GAAGCTTTTGAGAAGCAAAT+GGG + chr1_3:6041620-6041639 Msa0099770:exon 35.0%
!! AAGCTTTTGAGAAGCAAATG+GGG + chr1_3:6041621-6041640 Msa0099770:exon 35.0%
TCCTAGAAACAAGCTTCATA+GGG - chr1_3:6041884-6041903 None:intergenic 35.0%
ATCCTAGAAACAAGCTTCAT+AGG - chr1_3:6041885-6041904 None:intergenic 35.0%
CAAACACGTCTTCAATCTAA+AGG - chr1_3:6042016-6042035 None:intergenic 35.0%
CTTTAGATTGAAGACGTGTT+TGG + chr1_3:6042014-6042033 Msa0099770:intron 35.0%
ATGACACTCTCAATTCAAAC+GGG + chr1_3:6042102-6042121 Msa0099770:intron 35.0%
AAATTAGTCCCTGACATTGT+AGG - chr1_3:6042370-6042389 None:intergenic 35.0%
TACAATGTCAGGGACTAATT+TGG + chr1_3:6042369-6042388 Msa0099770:intron 35.0%
TCATATGTTAGTGTGAGATG+GGG + chr1_3:6042448-6042467 Msa0099770:intron 35.0%
TCATCATCTTCACTAGTAAC+GGG - chr1_3:6042598-6042617 None:intergenic 35.0%
TGCATTCACTAACTTCAAAC+AGG + chr1_3:6042618-6042637 Msa0099770:CDS 35.0%
AAGAACACACATTCAGCTAT+AGG - chr1_3:6042693-6042712 None:intergenic 35.0%
TGAAATGCCACACACAAAAA+AGG - chr1_3:6042723-6042742 None:intergenic 35.0%
AGTTGAAAACGCAAAAGAAG+AGG + chr1_3:6042886-6042905 Msa0099770:intron 35.0%
TAGCTATTGTTTCGTCTTTG+TGG + chr1_3:6042927-6042946 Msa0099770:intron 35.0%
! CGAGTTGTTCATTTTCTGAT+TGG + chr1_3:6043059-6043078 Msa0099770:intron 35.0%
! GTTGTTCATTTTCTGATTGG+TGG + chr1_3:6043062-6043081 Msa0099770:intron 35.0%
TGATCTTTGATGTAAAGGGT+TGG + chr1_3:6043139-6043158 Msa0099770:intron 35.0%
AGATGCAGTCTCTTCTATTA+TGG + chr1_3:6043353-6043372 Msa0099770:intron 35.0%
!! TTAAAACACCGCTTTGAAAC+TGG - chr1_3:6043442-6043461 None:intergenic 35.0%
TTGCTTTGCTTATATAGGGA+AGG + chr1_3:6043551-6043570 Msa0099770:intron 35.0%
TGCTTTGCTTATATAGGGAA+GGG + chr1_3:6043552-6043571 Msa0099770:intron 35.0%
ACATTAGAAGCCAGTTTGTA+GGG + chr1_3:6043640-6043659 Msa0099770:intron 35.0%
AAAGCTTATCCAAGATCTGA+CGG + chr1_3:6043684-6043703 Msa0099770:intron 35.0%
AGTAGCTCAAAATTGACAGT+GGG - chr1_3:6043714-6043733 None:intergenic 35.0%
AAGTAGCTCAAAATTGACAG+TGG - chr1_3:6043715-6043734 None:intergenic 35.0%
! ACTGTCAATTTTGAGCTACT+TGG + chr1_3:6043714-6043733 Msa0099770:intron 35.0%
! CTGTCAATTTTGAGCTACTT+GGG + chr1_3:6043715-6043734 Msa0099770:intron 35.0%
TTAATTCAACCCTGCAAAAC+CGG - chr1_3:6043857-6043876 None:intergenic 35.0%
AGGACCTCTTAAAATTTGGT+TGG - chr1_3:6043882-6043901 None:intergenic 35.0%
CAACAGGACCTCTTAAAATT+TGG - chr1_3:6043886-6043905 None:intergenic 35.0%
!! CAAATTTTAAGAGGTCCTGT+TGG + chr1_3:6043884-6043903 Msa0099770:intron 35.0%
ACTAGAGTAATAGCTCAAAC+GGG + chr1_3:6043972-6043991 Msa0099770:intron 35.0%
AAAATCACTTTGCAGATGCT+TGG - chr1_3:6044049-6044068 None:intergenic 35.0%
AGTCAACCAAGTAATTGCAA+TGG + chr1_3:6044122-6044141 Msa0099770:intron 35.0%
! AAAACTGGTGAGCAGTTTTA+CGG + chr1_3:6044207-6044226 Msa0099770:intron 35.0%
TCCTAGAAACTAATCCACTT+GGG + chr1_3:6044411-6044430 Msa0099770:intron 35.0%
TTACACAAACAAAAGCGAAG+AGG - chr1_3:6044591-6044610 None:intergenic 35.0%
TAGTTAGTCATAAGGTCTGT+AGG + chr1_3:6044634-6044653 Msa0099770:intron 35.0%
AAATAGCAGTTATAGTGGCT+CGG + chr1_3:6044723-6044742 Msa0099770:intron 35.0%
TCAAATTCCTCAACACTAGA+GGG - chr1_3:6044850-6044869 None:intergenic 35.0%
TTCAAATTCCTCAACACTAG+AGG - chr1_3:6044851-6044870 None:intergenic 35.0%
GTATGCATGTCATGTTTATC+TGG + chr1_3:6044950-6044969 Msa0099770:intron 35.0%
TACAAAGTGTGGTCAAATAG+TGG + chr1_3:6045033-6045052 Msa0099770:intron 35.0%
! TGTACCAGTGTTGTTAATCA+CGG - chr1_3:6045118-6045137 None:intergenic 35.0%
!!! CTGTAAATGTGTTTTGATGC+AGG + chr1_3:6045260-6045279 Msa0099770:intron 35.0%
!!! TGCATGATTTTTTGGTTGTC+TGG - chr1_3:6045367-6045386 None:intergenic 35.0%
!!! ACAGTTTTAGTTCAGTTCCT+AGG + chr1_3:6045391-6045410 Msa0099770:three_prime_UTR 35.0%
!!! CAGTTTTAGTTCAGTTCCTA+GGG + chr1_3:6045392-6045411 Msa0099770:three_prime_UTR 35.0%
AATAGAACTTTGCCGTGTAT+TGG + chr1_3:6045454-6045473 Msa0099770:three_prime_UTR 35.0%
GCTACAATATGTAGCTTCAA+TGG - chr1_3:6045543-6045562 None:intergenic 35.0%
CCAGTATGTTACACATGTTA+TGG - chr1_3:6045747-6045766 None:intergenic 35.0%
CCATAACATGTGTAACATAC+TGG + chr1_3:6045744-6045763 Msa0099770:three_prime_UTR 35.0%
ATGTCATATTTACACGAGGA+AGG + chr1_3:6045806-6045825 Msa0099770:three_prime_UTR 35.0%
GAATACAATGAAGCAACCGA+CGG + chr1_3:6041647-6041666 Msa0099770:CDS 40.0%
GAATAGTTTCACAAGTCCGT+CGG - chr1_3:6041666-6041685 None:intergenic 40.0%
! TGAAAAGTGTAATATGGGGG+GGG - chr1_3:6041776-6041795 None:intergenic 40.0%
! GTGAAAAGTGTAATATGGGG+GGG - chr1_3:6041777-6041796 None:intergenic 40.0%
! GGTGAAAAGTGTAATATGGG+GGG - chr1_3:6041778-6041797 None:intergenic 40.0%
! GGGTGAAAAGTGTAATATGG+GGG - chr1_3:6041779-6041798 None:intergenic 40.0%
! GGGGTGAAAAGTGTAATATG+GGG - chr1_3:6041780-6041799 None:intergenic 40.0%
! GGGGGTGAAAAGTGTAATAT+GGG - chr1_3:6041781-6041800 None:intergenic 40.0%
! AGGGGGTGAAAAGTGTAATA+TGG - chr1_3:6041782-6041801 None:intergenic 40.0%
!!! CACTGATATTGGTTTTCTGC+AGG + chr1_3:6041854-6041873 Msa0099770:intron 40.0%
! ACCCTATGAAGCTTGTTTCT+AGG + chr1_3:6041880-6041899 Msa0099770:intron 40.0%
AATGTCAGGGACTAATTTGG+TGG + chr1_3:6042372-6042391 Msa0099770:intron 40.0%
CATCATCTTCACTAGTAACG+GGG - chr1_3:6042597-6042616 None:intergenic 40.0%
!!! TGATGTGCCTTTTTTGTGTG+TGG + chr1_3:6042713-6042732 Msa0099770:intron 40.0%
TGAAAACGCAAAAGAAGAGG+TGG + chr1_3:6042889-6042908 Msa0099770:intron 40.0%
GAAAACGCAAAAGAAGAGGT+GGG + chr1_3:6042890-6042909 Msa0099770:intron 40.0%
TTAAAATGTTGTCGTGGGGA+TGG + chr1_3:6043011-6043030 Msa0099770:intron 40.0%
GGATGGTTTATTGGTAGAGA+AGG + chr1_3:6043028-6043047 Msa0099770:intron 40.0%
GGTTTATTGGTAGAGAAGGA+AGG + chr1_3:6043032-6043051 Msa0099770:intron 40.0%
AATAGCCAAGTGAAGAGTGA+TGG + chr1_3:6043378-6043397 Msa0099770:intron 40.0%
AGTTACCATCACTCTTCACT+TGG - chr1_3:6043386-6043405 None:intergenic 40.0%
AAAGGTTGCCAGTTTCAAAG+CGG + chr1_3:6043431-6043450 Msa0099770:intron 40.0%
GCTTTGCTTATATAGGGAAG+GGG + chr1_3:6043553-6043572 Msa0099770:intron 40.0%
ATCTCATCCAATATGGGAAC+AGG - chr1_3:6043581-6043600 None:intergenic 40.0%
! CAAACTGGCTTCTAATGTGA+GGG - chr1_3:6043638-6043657 None:intergenic 40.0%
! ACAAACTGGCTTCTAATGTG+AGG - chr1_3:6043639-6043658 None:intergenic 40.0%
CACATTAGAAGCCAGTTTGT+AGG + chr1_3:6043639-6043658 Msa0099770:intron 40.0%
AAGCTTATCCAAGATCTGAC+GGG + chr1_3:6043685-6043704 Msa0099770:intron 40.0%
AAGACTGAACATTTGCAGCA+TGG - chr1_3:6043741-6043760 None:intergenic 40.0%
TGCTGCAAATGTTCAGTCTT+AGG + chr1_3:6043741-6043760 Msa0099770:intron 40.0%
GTTCCTCATTGGATGACATA+TGG + chr1_3:6043783-6043802 Msa0099770:intron 40.0%
!!! AGGTCCTGTTGGTTTTTAGT+TGG + chr1_3:6043895-6043914 Msa0099770:intron 40.0%
TCTGCCAACTAAAAACCAAC+AGG - chr1_3:6043902-6043921 None:intergenic 40.0%
!!! GTTTTTAGTTGGCAGAACGT+GGG + chr1_3:6043906-6043925 Msa0099770:intron 40.0%
AGGGAGAAGGTAAAATACAG+AGG + chr1_3:6043930-6043949 Msa0099770:intron 40.0%
TTGTGCAGGTGATTGATGAA+TGG + chr1_3:6044166-6044185 Msa0099770:intron 40.0%
! GAGCAGTTTTACGGACTTTT+TGG + chr1_3:6044216-6044235 Msa0099770:intron 40.0%
TCAAATGTACAGCATGTGCA+TGG - chr1_3:6044254-6044273 None:intergenic 40.0%
CTCCTAGAAACTAATCCACT+TGG + chr1_3:6044410-6044429 Msa0099770:intron 40.0%
!! ACCCAAGTGGATTAGTTTCT+AGG - chr1_3:6044415-6044434 None:intergenic 40.0%
AGAAACTAATCCACTTGGGT+TGG + chr1_3:6044415-6044434 Msa0099770:intron 40.0%
CAAAAGCGAAGAGGAGAAAA+AGG - chr1_3:6044582-6044601 None:intergenic 40.0%
TCGATTCCTTCAATGAAGAG+AGG + chr1_3:6044669-6044688 Msa0099770:intron 40.0%
TTATACTAAACAGGGTGACG+CGG - chr1_3:6044793-6044812 None:intergenic 40.0%
! ACATCAAGTGTTGTCAATCG+TGG - chr1_3:6044895-6044914 None:intergenic 40.0%
TGCATGTCATGTTTATCTGG+CGG + chr1_3:6044953-6044972 Msa0099770:intron 40.0%
GAACCACAAGATTCTGATTC+TGG + chr1_3:6045287-6045306 Msa0099770:CDS 40.0%
TCTCCAGAATCAGAATCTTG+TGG - chr1_3:6045293-6045312 None:intergenic 40.0%
TTCTATCTTGCTCACCACAA+TGG - chr1_3:6045721-6045740 None:intergenic 40.0%
TTGTGAAGGAATGACATGAG+TGG + chr1_3:6045864-6045883 Msa0099770:three_prime_UTR 40.0%
GTAAACCACCGAATTCGACA+TGG + chr1_3:6042073-6042092 Msa0099770:intron 45.0%
GAAAACCATGTCGAATTCGG+TGG - chr1_3:6042081-6042100 None:intergenic 45.0%
GACAGAGTCAGTGACATTTG+TGG - chr1_3:6042148-6042167 None:intergenic 45.0%
! TATCAGTCCTTCTGAGAGTG+CGG + chr1_3:6042180-6042199 Msa0099770:intron 45.0%
ATTGTAGGCCACTGTCGAAT+AGG - chr1_3:6042355-6042374 None:intergenic 45.0%
GTGAGATGGGGATAAGATCT+AGG + chr1_3:6042460-6042479 Msa0099770:intron 45.0%
CAGCGTATGCAATCATCTGT+GGG + chr1_3:6042568-6042587 Msa0099770:CDS 45.0%
AAGAGGTGGGACATGTCATA+CGG + chr1_3:6042903-6042922 Msa0099770:intron 45.0%
TTGTCGTGGGGATGGTTTAT+TGG + chr1_3:6043019-6043038 Msa0099770:intron 45.0%
! TTGGTTGTTTGCTGGCAAGA+GGG - chr1_3:6043473-6043492 None:intergenic 45.0%
! ATTGGTTGTTTGCTGGCAAG+AGG - chr1_3:6043474-6043493 None:intergenic 45.0%
!! TGTGAAAGGTACCCAAAGCT+TGG - chr1_3:6043531-6043550 None:intergenic 45.0%
AATGTTCAGTCTTAGGCGTG+AGG + chr1_3:6043748-6043767 Msa0099770:intron 45.0%
ATGTTCAGTCTTAGGCGTGA+GGG + chr1_3:6043749-6043768 Msa0099770:intron 45.0%
AGGCCATATGTCATCCAATG+AGG - chr1_3:6043789-6043808 None:intergenic 45.0%
!!! GGTTTTTAGTTGGCAGAACG+TGG + chr1_3:6043905-6043924 Msa0099770:intron 45.0%
ACAGCATGTGCATGGACAAT+TGG - chr1_3:6044246-6044265 None:intergenic 45.0%
!! TAAGGTCTGTAGGAGTGCTT+AGG + chr1_3:6044644-6044663 Msa0099770:intron 45.0%
!! AAGGTCTGTAGGAGTGCTTA+GGG + chr1_3:6044645-6044664 Msa0099770:intron 45.0%
CACTCTCCTCTCTTCATTGA+AGG - chr1_3:6044678-6044697 None:intergenic 45.0%
CTTCAATGAAGAGAGGAGAG+TGG + chr1_3:6044676-6044695 Msa0099770:intron 45.0%
TAGTGGCTATAGTGCTGTAG+CGG + chr1_3:6045050-6045069 Msa0099770:intron 45.0%
! CCAGTGTTGTTAATCACGGA+TGG - chr1_3:6045114-6045133 None:intergenic 45.0%
CCATCCGTGATTAACAACAC+TGG + chr1_3:6045111-6045130 Msa0099770:intron 45.0%
GTGAGTGTTTCGATCTGTGT+TGG + chr1_3:6045499-6045518 Msa0099770:three_prime_UTR 45.0%
! TAACTTGTGTGCTTCTGCTG+TGG + chr1_3:6045624-6045643 Msa0099770:three_prime_UTR 45.0%
GTGTGCACACAGAAACAGTA+TGG + chr1_3:6045674-6045693 Msa0099770:three_prime_UTR 45.0%
GCACACAGAAACAGTATGGT+AGG + chr1_3:6045678-6045697 Msa0099770:three_prime_UTR 45.0%
! CTGCAAGCTGCATTTTGTGA+AGG + chr1_3:6045850-6045869 Msa0099770:three_prime_UTR 45.0%
TGAGCTTCTTGCTGCTAAGC+AGG + chr1_3:6041950-6041969 Msa0099770:intron 50.0%
! ATCAGTCCTTCTGAGAGTGC+GGG + chr1_3:6042181-6042200 Msa0099770:intron 50.0%
GACAGTGGCCTACAATGTCA+GGG + chr1_3:6042359-6042378 Msa0099770:intron 50.0%
! GTTTGCTTGTTGAGCTGTGC+AGG + chr1_3:6042495-6042514 Msa0099770:intron 50.0%
GCAGCGTATGCAATCATCTG+TGG + chr1_3:6042567-6042586 Msa0099770:CDS 50.0%
AGGGGAACCTGTTCCCATAT+TGG + chr1_3:6043571-6043590 Msa0099770:intron 50.0%
TGTTCAGTCTTAGGCGTGAG+GGG + chr1_3:6043750-6043769 Msa0099770:intron 50.0%
GTGCGTTGAGTGTTCCTCAT+TGG + chr1_3:6043772-6043791 Msa0099770:intron 50.0%
TGCAAAACCGGCAGCTTGTA+AGG - chr1_3:6043845-6043864 None:intergenic 50.0%
!!! TTACAAGCTGCCGGTTTTGC+AGG + chr1_3:6043844-6043863 Msa0099770:intron 50.0%
!!! TACAAGCTGCCGGTTTTGCA+GGG + chr1_3:6043845-6043864 Msa0099770:intron 50.0%
TTAGTTGGCAGAACGTGGGA+AGG + chr1_3:6043910-6043929 Msa0099770:intron 50.0%
TAGTTGGCAGAACGTGGGAA+GGG + chr1_3:6043911-6043930 Msa0099770:intron 50.0%
GATCTCAAGCCAACACCACT+AGG - chr1_3:6044441-6044460 None:intergenic 50.0%
!! GTGGTGTTGGCTTGAGATCT+TGG + chr1_3:6044442-6044461 Msa0099770:intron 50.0%
CTTGGAGTGTGCTCTTCTCA+AGG + chr1_3:6044460-6044479 Msa0099770:intron 50.0%
TGCTCAACCCTCTAGTGTTG+AGG + chr1_3:6044840-6044859 Msa0099770:intron 50.0%
GTGTTGTCAATCGTGGAACG+TGG - chr1_3:6044888-6044907 None:intergenic 50.0%
GCAGCGAAATGAAGACAGCA+TGG - chr1_3:6045172-6045191 None:intergenic 50.0%
GCTGTCTTCATTTCGCTGCA+TGG + chr1_3:6045173-6045192 Msa0099770:intron 50.0%
TTGTGTGCTTCTGCTGTGGA+AGG + chr1_3:6045628-6045647 Msa0099770:three_prime_UTR 50.0%
TGTGTGCTTCTGCTGTGGAA+GGG + chr1_3:6045629-6045648 Msa0099770:three_prime_UTR 50.0%
TTTGGTCCCGCACTCTCAGA+AGG - chr1_3:6042190-6042209 None:intergenic 55.0%
CACAGGGACCTATTCGACAG+TGG + chr1_3:6042344-6042363 Msa0099770:intron 55.0%
CGACAGTGGCCTACAATGTC+AGG + chr1_3:6042358-6042377 Msa0099770:intron 55.0%
TTCCTCGCCTTACAAGCTGC+CGG + chr1_3:6043835-6043854 Msa0099770:intron 55.0%
!! ATCCACTTGGGTTGGCCTAG+TGG + chr1_3:6044423-6044442 Msa0099770:intron 55.0%
!! TTGGGTTGGCCTAGTGGTGT+TGG + chr1_3:6044429-6044448 Msa0099770:intron 55.0%
! ATAGTGGCTCGGCAGTGTTG+TGG + chr1_3:6044734-6044753 Msa0099770:intron 55.0%
! GTGGGTTGCCCGTCAGATCT+TGG - chr1_3:6043696-6043715 None:intergenic 60.0%
AACCGGCAGCTTGTAAGGCG+AGG - chr1_3:6043840-6043859 None:intergenic 60.0%
GCAGAACGTGGGAAGGGAGA+AGG + chr1_3:6043917-6043936 Msa0099770:intron 60.0%
CACCACTAGGCCAACCCAAG+TGG - chr1_3:6044428-6044447 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 6041609 6045955 6041609 ID=Msa0099770;Name=Msa0099770
chr1_3 mRNA 6041609 6045955 6041609 ID=Msa0099770-mRNA-1;Parent=Msa0099770;Name=Msa0099770-mRNA-1;_AED=0.05;_eAED=0.05;_QI=29|0.66|1|1|0.66|1|4|600|119
chr1_3 exon 6041609 6041760 6041609 ID=Msa0099770-mRNA-1:exon:2294;Parent=Msa0099770-mRNA-1
chr1_3 exon 6042517 6042639 6042517 ID=Msa0099770-mRNA-1:exon:2295;Parent=Msa0099770-mRNA-1
chr1_3 exon 6044174 6044213 6044174 ID=Msa0099770-mRNA-1:exon:2296;Parent=Msa0099770-mRNA-1
chr1_3 exon 6045282 6045955 6045282 ID=Msa0099770-mRNA-1:exon:2297;Parent=Msa0099770-mRNA-1
chr1_3 five_prime_UTR 6041609 6041637 6041609 ID=Msa0099770-mRNA-1:five_prime_utr;Parent=Msa0099770-mRNA-1
chr1_3 CDS 6041638 6041760 6041638 ID=Msa0099770-mRNA-1:cds;Parent=Msa0099770-mRNA-1
chr1_3 CDS 6042517 6042639 6042517 ID=Msa0099770-mRNA-1:cds;Parent=Msa0099770-mRNA-1
chr1_3 CDS 6044174 6044213 6044174 ID=Msa0099770-mRNA-1:cds;Parent=Msa0099770-mRNA-1
chr1_3 CDS 6045282 6045355 6045282 ID=Msa0099770-mRNA-1:cds;Parent=Msa0099770-mRNA-1
chr1_3 three_prime_UTR 6045356 6045955 6045356 ID=Msa0099770-mRNA-1:three_prime_utr;Parent=Msa0099770-mRNA-1
Gene Sequence

>Msa0099770

ATGGGGAATGAATACAATGAAGCAACCGACGGACTTGTGAAACTATTCAGAAAAGCAAATCACGATCTTGATATCGTTCAACACCGTCTTCAAACTGAATTTCAACAGCAGTACCCTGAAAATGATTTGATTGATAAAGCTCAGACAGTTCTTGTTGAGAATAGAAATTTAGTGCAGCGTATGCAATCATCTGTGGGAATCCCCGTTACTAGTGAAGATGATGATGCATTCACTAACTTCAAACAGGTGATTGATGAATGGACTGATCAAATAAGATCAAAAACTGGAAATGAACCACAAGATTCTGATTCTGGAGATCTCAACAAACTACTCTTTTCAGCTATAGTTCAAAGTAATTGA
Protein sequence

>Msa0099770

MGNEYNEATDGLVKLFRKANHDLDIVQHRLQTEFQQQYPENDLIDKAQTVLVENRNLVQRMQSSVGIPVTSEDDDAFTNFKQVIDEWTDQIRSKTGNEPQDSDSGDLNKLLFSAIVQSN*