AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1357730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1357730 MtrunA17_Chr7g0268501 100.000 133 0 0 1 133 188 320 3.55e-95 276
Msa1357730 MtrunA17_Chr7g0268521 100.000 133 0 0 1 133 188 320 3.55e-95 276
Msa1357730 MtrunA17_Chr7g0268491 92.941 85 6 0 49 133 13 97 1.00e-52 161
Msa1357730 MtrunA17_Chr4g0024121 54.074 135 60 2 1 133 192 326 4.88e-45 149
Msa1357730 MtrunA17_Chr2g0322861 52.206 136 62 2 1 133 251 386 4.70e-43 145
Msa1357730 MtrunA17_Chr3g0128061 47.407 135 69 2 1 133 192 326 1.09e-33 119
Msa1357730 MtrunA17_Chr1g0164891 42.963 135 75 2 1 133 258 392 1.30e-31 115
Msa1357730 MtrunA17_Chr1g0148021 44.776 134 73 1 1 133 195 328 3.77e-31 113
Msa1357730 MtrunA17_Chr1g0194151 42.647 136 75 2 1 133 198 333 6.39e-31 112
Msa1357730 MtrunA17_Chr2g0292261 45.588 136 71 2 1 133 195 330 1.55e-30 112
Msa1357730 MtrunA17_Chr2g0292241 44.853 136 72 2 1 133 195 330 2.37e-30 111
Msa1357730 MtrunA17_Chr7g0258811 45.588 136 71 2 1 133 236 371 3.32e-30 111
Msa1357730 MtrunA17_Chr5g0401431 42.857 133 75 1 1 132 196 328 5.15e-30 110
Msa1357730 MtrunA17_Chr4g0070951 44.203 138 72 1 1 133 192 329 5.16e-30 110
Msa1357730 MtrunA17_Chr4g0072091 44.030 134 74 1 1 133 190 323 7.44e-30 109
Msa1357730 MtrunA17_Chr1g0175561 44.853 136 72 2 1 133 191 326 1.18e-29 109
Msa1357730 MtrunA17_Chr8g0371381 42.222 135 69 3 2 133 191 319 3.04e-29 107
Msa1357730 MtrunA17_Chr2g0292251 43.382 136 74 2 1 133 194 329 8.37e-29 107
Msa1357730 MtrunA17_Chr2g0292211 42.647 136 75 2 1 133 206 341 9.87e-29 107
Msa1357730 MtrunA17_Chr2g0292271 44.118 136 73 2 1 133 195 330 2.33e-28 106
Msa1357730 MtrunA17_Chr1g0194271 38.462 143 78 2 1 133 198 340 2.72e-28 105
Msa1357730 MtrunA17_Chr7g0225221 41.791 134 73 2 1 133 191 320 4.58e-28 104
Msa1357730 MtrunA17_Chr5g0402581 42.963 135 75 2 1 133 192 326 8.97e-28 104
Msa1357730 MtrunA17_Chr2g0299691 43.571 140 71 3 1 133 220 358 1.91e-27 103
Msa1357730 MtrunA17_Chr5g0404721 42.963 135 75 2 1 133 196 330 2.33e-27 103
Msa1357730 MtrunA17_Chr4g0053871 42.857 133 70 2 1 132 196 323 1.05e-26 101
Msa1357730 MtrunA17_Chr2g0291751 40.441 136 78 2 1 133 195 330 1.97e-26 101
Msa1357730 MtrunA17_Chr6g0467161 41.045 134 71 3 1 133 210 336 2.34e-26 100
Msa1357730 MtrunA17_Chr8g0364291 38.519 135 81 2 1 133 190 324 3.81e-26 100
Msa1357730 MtrunA17_Chr5g0405991 42.029 138 65 6 2 133 188 316 4.21e-26 99.8
Msa1357730 MtrunA17_Chr8g0372601 42.963 135 75 2 1 133 196 330 1.46e-25 98.6
Msa1357730 MtrunA17_Chr2g0292181 39.706 136 79 2 1 133 204 339 1.55e-25 99.0
Msa1357730 MtrunA17_Chr1g0204011 41.791 134 73 3 2 133 192 322 1.97e-25 97.8
Msa1357730 MtrunA17_Chr2g0279061 39.130 138 79 1 1 133 129 266 2.21e-25 96.7
Msa1357730 MtrunA17_Chr2g0292151 41.176 136 77 2 1 133 195 330 2.34e-25 98.2
Msa1357730 MtrunA17_Chr2g0296621 39.552 134 77 2 1 133 211 341 2.48e-25 98.2
Msa1357730 MtrunA17_Chr2g0292111 40.441 136 78 2 1 133 195 330 5.88e-25 97.1
Msa1357730 MtrunA17_Chr2g0279041 39.130 138 79 1 1 133 188 325 6.01e-25 96.7
Msa1357730 MtrunA17_Chr2g0292101 42.647 136 75 2 1 133 191 326 6.02e-25 97.1
Msa1357730 MtrunA17_Chr5g0402571 38.519 135 81 2 1 133 192 326 1.13e-24 95.9
Msa1357730 MtrunA17_Chr2g0292291 42.336 137 75 3 1 133 196 332 2.41e-24 95.5
Msa1357730 MtrunA17_Chr1g0197411 36.765 136 82 3 1 133 196 330 9.54e-24 93.6
Msa1357730 MtrunA17_Chr2g0329641 35.971 139 83 2 1 133 197 335 1.21e-23 93.6
Msa1357730 MtrunA17_Chr1g0157051 37.879 132 78 2 2 132 189 317 2.24e-23 92.4
Msa1357730 MtrunA17_Chr4g0046131 36.567 134 82 3 1 131 211 344 2.67e-23 92.8
Msa1357730 MtrunA17_Chr2g0292281 41.912 136 66 4 1 133 91 216 7.09e-23 89.7
Msa1357730 MtrunA17_Chr6g0467121 39.552 134 73 3 1 133 237 363 2.10e-22 90.5
Msa1357730 MtrunA17_Chr7g0254201 36.567 134 80 3 1 133 183 312 2.44e-22 89.7
Msa1357730 MtrunA17_Chr2g0292091 39.706 136 79 2 1 133 190 325 4.27e-22 89.4
Msa1357730 MtrunA17_Chr2g0320091 37.778 135 75 3 1 133 191 318 9.14e-22 88.2
Msa1357730 MtrunA17_Chr4g0076211 38.519 135 78 3 1 133 202 333 1.29e-21 87.8
Msa1357730 MtrunA17_Chr6g0467251 35.821 134 78 3 1 133 202 328 1.41e-21 88.2
Msa1357730 MtrunA17_Chr2g0320071 36.567 134 77 2 1 133 191 317 2.51e-21 87.0
Msa1357730 MtrunA17_Chr1g0188431 35.821 134 76 3 1 133 203 327 1.08e-20 85.5
Msa1357730 MtrunA17_Chr3g0129051 37.879 132 72 3 1 129 194 318 2.31e-20 84.3
Msa1357730 MtrunA17_Chr2g0320101 36.567 134 77 2 1 133 191 317 2.66e-20 84.3
Msa1357730 MtrunA17_Chr3g0136751 36.496 137 82 3 1 133 187 322 3.89e-20 84.0
Msa1357730 MtrunA17_Chr6g0477071 36.567 134 75 5 1 133 218 342 9.60e-20 82.8
Msa1357730 MtrunA17_Chr1g0181511 34.559 136 80 3 2 133 193 323 1.30e-19 82.4
Msa1357730 MtrunA17_Chr4g0063671 33.094 139 87 1 1 133 182 320 1.44e-19 82.4
Msa1357730 MtrunA17_Chr4g0074851 35.036 137 80 4 1 133 197 328 1.49e-19 82.4
Msa1357730 MtrunA17_Chr1g0181481 33.577 137 82 3 1 133 192 323 1.60e-19 82.0
Msa1357730 MtrunA17_Chr4g0068621 38.235 136 78 4 1 133 204 336 1.63e-19 82.4
Msa1357730 MtrunA17_Chr7g0244461 34.328 134 80 2 1 133 186 312 1.63e-19 82.0
Msa1357730 MtrunA17_Chr1g0181431 33.577 137 82 3 1 133 192 323 1.92e-19 82.0
Msa1357730 MtrunA17_Chr2g0320051 37.778 135 75 2 1 133 191 318 2.78e-19 81.3
Msa1357730 MtrunA17_Chr4g0013721 35.075 134 83 3 1 133 266 396 4.35e-19 81.6
Msa1357730 MtrunA17_Chr2g0320061 36.296 135 77 2 1 133 191 318 5.09e-19 80.9
Msa1357730 MtrunA17_Chr1g0181501 32.847 137 83 3 1 133 58 189 5.64e-19 78.6
Msa1357730 MtrunA17_Chr1g0155611 39.706 136 79 2 1 133 212 347 6.66e-19 80.9
Msa1357730 MtrunA17_Chr3g0114451 33.582 134 81 2 1 133 192 318 4.66e-18 78.2
Msa1357730 MtrunA17_Chr4g0013171 33.582 134 81 2 1 133 195 321 7.40e-18 77.8
Msa1357730 MtrunA17_Chr3g0129061 34.848 132 76 3 1 129 194 318 8.60e-18 77.4
Msa1357730 MtrunA17_Chr2g0320031 34.815 135 79 2 1 133 192 319 2.14e-17 76.3
Msa1357730 MtrunA17_Chr4g0004591 30.921 152 85 2 1 132 204 355 2.82e-17 76.3
Msa1357730 MtrunA17_Chr4g0013181 32.836 134 82 2 1 133 227 353 2.85e-17 76.3
Msa1357730 MtrunA17_Chr6g0460711 33.582 134 85 3 1 133 184 314 3.11e-17 75.9
Msa1357730 MtrunA17_Chr4g0038161 35.714 140 74 3 1 133 190 320 4.56e-17 75.5
Msa1357730 MtrunA17_Chr4g0038151 35.000 140 75 3 1 133 191 321 7.39e-17 74.7
Msa1357730 MtrunA17_Chr3g0114461 34.074 135 80 3 1 133 65 192 8.19e-17 72.8
Msa1357730 MtrunA17_Chr7g0254231 32.090 134 83 3 1 133 185 311 1.44e-16 73.9
Msa1357730 MtrunA17_Chr5g0413051 30.714 140 87 4 1 133 241 377 1.62e-16 74.3
Msa1357730 MtrunA17_Chr3g0129041 34.559 136 79 3 1 133 194 322 1.92e-16 73.6
Msa1357730 MtrunA17_Chr5g0438021 33.088 136 81 3 1 133 197 325 2.19e-16 73.6
Msa1357730 MtrunA17_Chr3g0106901 32.090 134 83 2 1 133 197 323 2.57e-16 73.6
Msa1357730 MtrunA17_Chr4g0043391 34.058 138 80 3 1 133 193 324 2.80e-16 73.2
Msa1357730 MtrunA17_Chr3g0107881 35.294 136 78 3 1 133 196 324 2.92e-16 73.2
Msa1357730 MtrunA17_Chr7g0224371 35.294 136 75 4 1 133 197 322 4.17e-16 72.8
Msa1357730 MtrunA17_Chr1g0213061 32.847 137 81 3 1 133 192 321 5.12e-16 72.8
Msa1357730 MtrunA17_Chr7g0244451 32.090 134 83 2 1 133 190 316 5.59e-16 72.4
Msa1357730 MtrunA17_Chr5g0432441 32.847 137 81 3 1 133 197 326 1.94e-15 70.9
Msa1357730 MtrunA17_Chr4g0013191 33.088 136 81 3 1 133 212 340 2.30e-15 70.9
Msa1357730 MtrunA17_Chr4g0038171 33.333 141 77 3 1 133 190 321 2.49e-15 70.9
Msa1357730 MtrunA17_Chr6g0451571 32.353 136 86 4 1 133 202 334 2.68e-15 70.9
Msa1357730 MtrunA17_Chr5g0400331 31.429 140 89 2 1 133 192 331 4.59e-15 70.1
Msa1357730 MtrunA17_Chr4g0013821 30.597 134 89 3 1 133 194 324 4.99e-15 70.1
Msa1357730 MtrunA17_Chr4g0013201 32.090 134 83 2 1 133 212 338 9.32e-15 69.3
Msa1357730 MtrunA17_Chr4g0075241 33.088 136 85 4 1 133 195 327 1.13e-14 68.9
Msa1357730 MtrunA17_Chr2g0278671 32.374 139 87 2 1 133 186 323 2.39e-14 67.8
Msa1357730 MtrunA17_Chr5g0432331 36.496 137 74 5 1 133 225 352 4.51e-14 67.4
Msa1357730 MtrunA17_Chr3g0106921 30.882 136 84 3 1 133 192 320 1.64e-13 65.5
Msa1357730 MtrunA17_Chr5g0432351 35.036 137 76 5 1 133 194 321 6.67e-13 63.9
Msa1357730 MtrunA17_Chr2g0316481 26.543 162 86 4 1 130 243 403 7.75e-13 63.9
Msa1357730 MtrunA17_Chr2g0316191 26.380 163 86 4 1 130 243 404 2.15e-12 62.8
Msa1357730 MtrunA17_Chr2g0316791 24.691 162 89 3 1 130 243 403 2.23e-12 62.8
Msa1357730 MtrunA17_Chr2g0316831 26.543 162 86 4 1 130 279 439 3.61e-12 62.0
Msa1357730 MtrunA17_Chr2g0316771 23.457 162 91 3 1 130 23 183 7.75e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1357730 AT4G11290.1 56.618 136 56 2 1 133 191 326 1.21e-45 150
Msa1357730 AT1G05260.1 52.941 136 61 2 1 133 190 325 2.23e-43 145
Msa1357730 AT2G39040.1 50.000 138 64 2 1 133 213 350 1.82e-41 140
Msa1357730 AT5G15180.1 50.370 135 64 2 1 133 196 329 1.21e-39 135
Msa1357730 AT3G21770.1 49.630 135 65 2 1 132 193 327 1.40e-39 135
Msa1357730 AT3G01190.1 50.370 135 64 2 1 133 188 321 1.78e-39 135
Msa1357730 AT1G05240.1 47.059 136 67 2 2 133 191 325 2.96e-38 132
Msa1357730 AT1G05250.1 47.059 136 67 2 2 133 191 325 2.96e-38 132
Msa1357730 AT5G64120.1 44.030 134 73 2 1 133 196 328 7.52e-32 115
Msa1357730 AT4G30170.1 45.522 134 72 1 1 133 192 325 1.08e-31 114
Msa1357730 AT2G18980.1 44.030 134 74 1 1 133 190 323 4.15e-31 113
Msa1357730 AT2G24800.1 46.377 138 68 4 1 133 193 329 1.08e-30 112
Msa1357730 AT2G18140.1 41.481 135 77 2 1 133 201 335 3.52e-30 111
Msa1357730 AT5G19880.1 42.336 137 75 3 1 133 193 329 3.57e-30 110
Msa1357730 AT4G37530.1 42.537 134 76 1 1 133 196 329 1.12e-29 109
Msa1357730 AT5G17820.1 38.806 134 77 2 1 133 184 313 7.93e-29 107
Msa1357730 AT4G31760.1 43.066 137 73 3 1 133 191 326 1.81e-28 106
Msa1357730 AT5G06720.1 44.853 136 72 2 1 133 198 333 1.92e-28 106
Msa1357730 AT5G14130.1 42.537 134 75 2 1 133 198 330 1.99e-28 106
Msa1357730 AT2G18150.1 40.741 135 78 2 1 133 202 336 2.43e-28 106
Msa1357730 AT5G17820.2 38.806 134 77 2 1 133 237 366 2.65e-28 106
Msa1357730 AT5G06730.1 45.588 136 71 2 1 133 199 334 2.75e-28 106
Msa1357730 AT5G14130.2 42.537 134 75 2 1 133 239 371 3.63e-28 106
Msa1357730 AT4G37520.2 40.299 134 79 1 1 133 193 326 2.00e-27 103
Msa1357730 AT4G37520.1 40.299 134 79 1 1 133 196 329 2.14e-27 103
Msa1357730 AT1G71695.1 42.857 133 69 3 2 133 213 339 3.55e-27 103
Msa1357730 AT5G19890.1 40.580 138 75 2 1 133 192 327 4.88e-27 102
Msa1357730 AT5G39580.1 39.552 134 79 2 1 133 187 319 1.45e-26 101
Msa1357730 AT2G38390.1 43.382 136 74 2 1 133 198 333 1.75e-26 101
Msa1357730 AT4G36430.1 38.519 135 81 2 1 133 196 330 3.35e-26 100
Msa1357730 AT5G67400.1 39.552 134 80 1 1 133 196 329 4.01e-26 100
Msa1357730 AT3G49960.1 41.045 134 78 1 1 133 196 329 4.18e-26 100
Msa1357730 AT5G64110.1 37.956 137 81 1 1 133 194 330 7.76e-26 99.8
Msa1357730 AT5G66390.1 41.912 136 75 3 1 133 199 333 1.38e-25 99.0
Msa1357730 AT4G08770.1 38.235 136 80 2 2 133 192 327 2.12e-25 98.6
Msa1357730 AT5G64100.1 36.496 137 81 2 1 133 197 331 5.93e-25 97.1
Msa1357730 AT3G32980.1 38.686 137 78 2 2 133 199 334 1.22e-24 96.7
Msa1357730 AT2G38380.1 41.176 136 77 2 1 133 198 333 3.34e-24 95.5
Msa1357730 AT3G50990.1 39.259 135 80 1 1 133 207 341 3.79e-23 92.4
Msa1357730 AT3G03670.1 34.328 134 87 1 1 133 188 321 7.76e-23 91.3
Msa1357730 AT1G34510.1 35.821 134 75 3 1 133 186 309 8.81e-23 91.3
Msa1357730 AT5G24070.1 36.691 139 82 2 1 133 197 335 9.94e-23 91.3
Msa1357730 AT1G30870.1 34.307 137 83 3 1 133 212 345 2.14e-22 90.5
Msa1357730 AT3G49110.1 35.766 137 84 1 1 133 200 336 2.20e-22 90.5
Msa1357730 AT1G49570.1 40.602 133 75 3 1 129 214 346 2.52e-22 90.5
Msa1357730 AT4G08780.1 35.294 136 84 1 2 133 192 327 4.52e-22 89.7
Msa1357730 AT3G49120.1 36.029 136 83 1 2 133 200 335 1.02e-21 89.0
Msa1357730 AT1G14550.1 37.778 135 76 3 1 133 193 321 1.69e-21 87.8
Msa1357730 AT1G14540.1 37.037 135 77 2 1 133 187 315 3.18e-21 87.0
Msa1357730 AT2G41480.1 33.813 139 86 1 1 133 190 328 3.47e-21 87.0
Msa1357730 AT5G22410.1 33.333 132 85 2 1 129 190 321 1.03e-20 85.9
Msa1357730 AT5G42180.1 34.848 132 82 2 2 132 188 316 1.80e-20 85.1
Msa1357730 AT4G17690.1 36.029 136 78 4 1 133 193 322 2.01e-20 85.1
Msa1357730 AT4G26010.1 35.338 133 75 3 2 133 187 309 3.16e-20 84.3
Msa1357730 AT5G51890.1 39.552 134 77 3 2 133 191 322 3.21e-20 84.3
Msa1357730 AT4G26010.2 35.075 134 76 3 1 133 214 337 3.65e-20 84.3
Msa1357730 AT4G33870.1 32.530 166 76 4 1 130 233 398 8.41e-20 84.0
Msa1357730 AT1G77100.1 32.609 138 82 2 1 133 188 319 1.00e-19 83.2
Msa1357730 AT2G22420.1 35.075 134 83 3 1 133 189 319 3.51e-19 81.6
Msa1357730 AT4G21960.1 34.307 137 81 4 1 133 196 327 4.09e-19 81.6
Msa1357730 AT4G33420.1 35.821 134 76 3 1 133 201 325 6.84e-19 80.9
Msa1357730 AT1G44970.1 38.971 136 80 2 1 133 211 346 7.68e-19 80.9
Msa1357730 AT4G33420.2 35.821 134 76 3 1 133 248 372 9.93e-19 80.9
Msa1357730 AT4G25980.1 33.813 139 86 2 1 133 188 326 1.14e-18 80.1
Msa1357730 AT4G37530.2 46.429 84 45 0 1 84 196 279 1.35e-18 79.3
Msa1357730 AT5G40150.1 36.765 136 77 4 1 133 199 328 1.78e-18 79.7
Msa1357730 AT2G34060.1 35.075 134 84 3 1 131 211 344 2.61e-18 79.3
Msa1357730 AT3G28200.1 34.074 135 81 3 1 133 188 316 6.31e-18 78.2
Msa1357730 AT1G24110.1 35.036 137 82 4 1 133 189 322 9.92e-18 77.8
Msa1357730 AT2G43480.1 30.216 139 91 2 1 133 197 335 1.29e-17 77.4
Msa1357730 AT2G37130.2 32.847 137 83 3 1 133 165 296 1.46e-17 77.0
Msa1357730 AT2G37130.1 32.847 137 83 3 1 133 196 327 1.85e-17 77.0
Msa1357730 AT5G47000.1 32.593 135 85 3 1 133 200 330 2.02e-17 77.0
Msa1357730 AT2G35380.2 32.857 140 87 2 1 133 108 247 6.83e-17 74.3
Msa1357730 AT2G35380.1 34.286 140 85 3 1 133 196 335 1.69e-16 74.3
Msa1357730 AT5G58390.1 33.333 132 78 3 1 129 188 312 8.20e-16 72.4
Msa1357730 AT3G17070.2 31.884 138 86 4 1 133 142 276 2.85e-15 70.5
Msa1357730 AT3G17070.1 31.884 138 86 4 1 133 205 339 5.89e-15 70.1
Msa1357730 AT4G16270.1 32.593 135 87 3 1 133 216 348 2.08e-14 68.6
Msa1357730 AT5G58400.1 33.083 133 77 4 1 129 197 321 2.87e-14 68.2
Msa1357730 AT1G68850.1 36.486 148 71 8 1 133 196 335 4.50e-14 67.8
Msa1357730 AT5G05340.1 31.618 136 83 3 1 133 196 324 6.59e-14 67.0

Find 24 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATGTTGTTGAGCTATT+TGG 0.173041 tig0016171:-340 None:intergenic
GTCACTTAGGCCTTGGCATT+TGG 0.297824 tig0016171:-293 None:intergenic
TAGAAACATTGTTAATGAAT+TGG 0.309617 tig0016171:+441 Msa1357730:CDS
TTCAAGAAATTTGCATAAGT+TGG 0.318870 tig0016171:-268 None:intergenic
AACAATGTTTCTAGATTGCT+TGG 0.397011 tig0016171:-431 None:intergenic
TTTATAGGAGCACACACAAT+TGG 0.421672 tig0016171:+169 None:intergenic
ATTGCAACTTATTCAGCAAC+AGG 0.429672 tig0016171:+200 Msa1357730:CDS
GTTCGAATGGAGAAATTAGA+AGG 0.434571 tig0016171:+536 Msa1357730:CDS
TGGTGGTGTCACTTAGGCCT+TGG 0.470093 tig0016171:-300 None:intergenic
CTGTTGTGGTGGTGTCACTT+AGG 0.496811 tig0016171:-306 None:intergenic
GAAGAGGATGGGAGCTATTG+AGG 0.498561 tig0016171:+504 Msa1357730:CDS
TTCTCAGTCAATGAAGAGGA+TGG 0.515611 tig0016171:+492 Msa1357730:CDS
GCATAAGTTGGATTCAGAGA+TGG 0.521194 tig0016171:-256 None:intergenic
GGAGCTATTGAGGTCCTCAC+TGG 0.526293 tig0016171:+514 Msa1357730:CDS
TCCTGTCCTTCTACAAAACA+AGG 0.528441 tig0016171:+375 Msa1357730:CDS
TGGATCCATCTCTACTGTTG+TGG 0.535692 tig0016171:-320 None:intergenic
CACCACCACAACAGTAGAGA+TGG 0.539448 tig0016171:+315 Msa1357730:CDS
ATCCATCTCTACTGTTGTGG+TGG 0.572153 tig0016171:-317 None:intergenic
GAGGTCCTCACTGGTTCGAA+TGG 0.575650 tig0016171:+523 Msa1357730:CDS
TCTCAGTCAATGAAGAGGAT+GGG 0.577113 tig0016171:+493 Msa1357730:CDS
TTCTTGAAGACCAAATGCCA+AGG 0.601738 tig0016171:+283 Msa1357730:CDS
GGAGCACACACAATTGGAGT+AGG 0.649757 tig0016171:+175 None:intergenic
CCTGTCCTTCTACAAAACAA+GGG 0.678901 tig0016171:+376 Msa1357730:CDS
TTTCTCCATTCGAACCAGTG+AGG 0.691397 tig0016171:-528 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGAAACATTGTTAATGAAT+TGG + tig0016171:441-460 Msa1357730:CDS 20.0%
! TTCAAGAAATTTGCATAAGT+TGG - tig0016171:271-290 None:intergenic 25.0%
TCAAATGTTGTTGAGCTATT+TGG - tig0016171:343-362 None:intergenic 30.0%
!! AACAATGTTTCTAGATTGCT+TGG - tig0016171:434-453 None:intergenic 30.0%
ATTGCAACTTATTCAGCAAC+AGG + tig0016171:200-219 Msa1357730:CDS 35.0%
! CTTTTCAACTTCACTGGAAA+AGG + tig0016171:223-242 Msa1357730:CDS 35.0%
! AGTTTTCTCAGTCAATGAAG+AGG + tig0016171:488-507 Msa1357730:CDS 35.0%
GTTCGAATGGAGAAATTAGA+AGG + tig0016171:536-555 Msa1357730:CDS 35.0%
! AACAGGCTTTTCAACTTCAC+TGG + tig0016171:217-236 Msa1357730:CDS 40.0%
! GCATAAGTTGGATTCAGAGA+TGG - tig0016171:259-278 None:intergenic 40.0%
TTCTTGAAGACCAAATGCCA+AGG + tig0016171:283-302 Msa1357730:CDS 40.0%
TCCTGTCCTTCTACAAAACA+AGG + tig0016171:375-394 Msa1357730:CDS 40.0%
CCTGTCCTTCTACAAAACAA+GGG + tig0016171:376-395 Msa1357730:CDS 40.0%
!!! AGAGACCCTTGTTTTGTAGA+AGG - tig0016171:384-403 None:intergenic 40.0%
TTCTCAGTCAATGAAGAGGA+TGG + tig0016171:492-511 Msa1357730:CDS 40.0%
TCTCAGTCAATGAAGAGGAT+GGG + tig0016171:493-512 Msa1357730:CDS 40.0%
ATCCATCTCTACTGTTGTGG+TGG - tig0016171:320-339 None:intergenic 45.0%
TGGATCCATCTCTACTGTTG+TGG - tig0016171:323-342 None:intergenic 45.0%
!!! CCCTTGTTTTGTAGAAGGAC+AGG - tig0016171:379-398 None:intergenic 45.0%
TTTCTCCATTCGAACCAGTG+AGG - tig0016171:531-550 None:intergenic 45.0%
GTCACTTAGGCCTTGGCATT+TGG - tig0016171:296-315 None:intergenic 50.0%
!! CTGTTGTGGTGGTGTCACTT+AGG - tig0016171:309-328 None:intergenic 50.0%
CACCACCACAACAGTAGAGA+TGG + tig0016171:315-334 Msa1357730:CDS 50.0%
GAAGAGGATGGGAGCTATTG+AGG + tig0016171:504-523 Msa1357730:CDS 50.0%
!! TGGTGGTGTCACTTAGGCCT+TGG - tig0016171:303-322 None:intergenic 55.0%
GGAGCTATTGAGGTCCTCAC+TGG + tig0016171:514-533 Msa1357730:CDS 55.0%
!! GAGGTCCTCACTGGTTCGAA+TGG + tig0016171:523-542 Msa1357730:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0016171 gene 178 576 178 ID=Msa1357730;Name=Msa1357730
tig0016171 mRNA 178 576 178 ID=Msa1357730-mRNA-1;Parent=Msa1357730;Name=Msa1357730-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|0|1|0|133
tig0016171 exon 178 576 178 ID=Msa1357730-mRNA-1:exon:10505;Parent=Msa1357730-mRNA-1
tig0016171 CDS 178 576 178 ID=Msa1357730-mRNA-1:cds;Parent=Msa1357730-mRNA-1
Gene Sequence

>Msa1357730

GCACACACAATTGGAGTAGGACATTGCAACTTATTCAGCAACAGGCTTTTCAACTTCACTGGAAAAGGTGATCAAGATCCATCTCTGAATCCAACTTATGCAAATTTCTTGAAGACCAAATGCCAAGGCCTAAGTGACACCACCACAACAGTAGAGATGGATCCAAATAGCTCAACAACATTTGACAATGACTATTATCCTGTCCTTCTACAAAACAAGGGTCTCTTCACATCAGATGCTGCTCTTCTAACAACCAAGCAATCTAGAAACATTGTTAATGAATTGGTTTCACAAAATAAATTCTTCACTGAGTTTTCTCAGTCAATGAAGAGGATGGGAGCTATTGAGGTCCTCACTGGTTCGAATGGAGAAATTAGAAGGAAGTGCTCTGTTGTTAAT
Protein sequence

>Msa1357730

AHTIGVGHCNLFSNRLFNFTGKGDQDPSLNPTYANFLKTKCQGLSDTTTTVEMDPNSSTTFDNDYYPVLLQNKGLFTSDAALLTTKQSRNIVNELVSQNKFFTEFSQSMKRMGAIEVLTGSNGEIRRKCSVVN