Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0227400 | Msa1358560 | 0.830833 | 2.349786e-55 | -8.615850e-47 |
| Msa1093330 | Msa1358560 | 0.804348 | 2.255616e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATCCCAGATCTTGTTAA+AGG | 0.270792 | tig0016349:+14289 | Msa1358560:intergenic |
| AGGGCTCCTTTAACAAGATC+TGG | 0.356611 | tig0016349:-14295 | Msa1358560:CDS |
| CAAGATCTGGGATTGGACTA+TGG | 0.396618 | tig0016349:-14282 | Msa1358560:CDS |
| GGACGGCAAGAACAGATGTT+AGG | 0.424689 | tig0016349:-14367 | Msa1358560:CDS |
| TGGAATACGTTGGGGATTGA+TGG | 0.439053 | tig0016349:-14319 | Msa1358560:CDS |
| AATTCTCATTCAGCCGTTGC+AGG | 0.440465 | tig0016349:-14249 | Msa1358560:CDS |
| CCACAGCACACATCATTACT+TGG | 0.442405 | tig0016349:-14224 | Msa1358560:CDS |
| GTACAACCATGGAATACGTT+GGG | 0.446927 | tig0016349:-14328 | Msa1358560:CDS |
| ATACGTTGGGGATTGATGGC+AGG | 0.469327 | tig0016349:-14315 | Msa1358560:CDS |
| GGTTGCTGTTAGGTGCAGTT+TGG | 0.480286 | tig0016349:-14388 | None:intergenic |
| CCTTTAACAAGATCTGGGAT+TGG | 0.523358 | tig0016349:-14289 | Msa1358560:CDS |
| GGGCTCCTTTAACAAGATCT+GGG | 0.530820 | tig0016349:-14294 | Msa1358560:CDS |
| GTGTGCTGTGGTTCCTGCAA+CGG | 0.541494 | tig0016349:+14236 | None:intergenic |
| GCTGTTAGGTGCAGTTTGGA+CGG | 0.546360 | tig0016349:-14384 | None:intergenic |
| CAGATGTTAGGAGTTACGTT+GGG | 0.558125 | tig0016349:-14355 | Msa1358560:CDS |
| TACGTTGGGGATTGATGGCA+GGG | 0.569598 | tig0016349:-14314 | Msa1358560:CDS |
| TCAATCCCCAACGTATTCCA+TGG | 0.595299 | tig0016349:+14322 | Msa1358560:intergenic |
| CGTTGGGAGGTGTACAACCA+TGG | 0.613849 | tig0016349:-14339 | Msa1358560:CDS |
| ACAGATGTTAGGAGTTACGT+TGG | 0.617439 | tig0016349:-14356 | Msa1358560:CDS |
| GAACACATTGCAAAATCTAG+AGG | 0.621854 | tig0016349:-14184 | Msa1358560:CDS |
| ATGTTAGGAGTTACGTTGGG+AGG | 0.623753 | tig0016349:-14352 | Msa1358560:CDS |
| TGTACAACCATGGAATACGT+TGG | 0.642869 | tig0016349:-14329 | Msa1358560:CDS |
| TACAACCATGGAATACGTTG+GGG | 0.648865 | tig0016349:-14327 | Msa1358560:CDS |
| CCAAGTAATGATGTGTGCTG+TGG | 0.697360 | tig0016349:+14224 | Msa1358550:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATTACTTGGAATTTTTGAA+TGG | - | tig0016349:14345-14364 | Msa1358560:CDS | 25.0% |
| GAACACATTGCAAAATCTAG+AGG | - | tig0016349:14372-14391 | Msa1358560:CDS | 35.0% | |
| ACAGATGTTAGGAGTTACGT+TGG | - | tig0016349:14200-14219 | Msa1358560:CDS | 40.0% | |
| CAGATGTTAGGAGTTACGTT+GGG | - | tig0016349:14201-14220 | Msa1358560:CDS | 40.0% | |
| TGTACAACCATGGAATACGT+TGG | - | tig0016349:14227-14246 | Msa1358560:CDS | 40.0% | |
| GTACAACCATGGAATACGTT+GGG | - | tig0016349:14228-14247 | Msa1358560:CDS | 40.0% | |
| TACAACCATGGAATACGTTG+GGG | - | tig0016349:14229-14248 | Msa1358560:CDS | 40.0% | |
| CCAATCCCAGATCTTGTTAA+AGG | + | tig0016349:14270-14289 | Msa1358560:intergenic | 40.0% | |
| ! | CCTTTAACAAGATCTGGGAT+TGG | - | tig0016349:14267-14286 | Msa1358560:CDS | 40.0% |
| ATGTTAGGAGTTACGTTGGG+AGG | - | tig0016349:14204-14223 | Msa1358560:CDS | 45.0% | |
| TCAATCCCCAACGTATTCCA+TGG | + | tig0016349:14237-14256 | Msa1358560:intergenic | 45.0% | |
| TGGAATACGTTGGGGATTGA+TGG | - | tig0016349:14237-14256 | Msa1358560:CDS | 45.0% | |
| AGGGCTCCTTTAACAAGATC+TGG | - | tig0016349:14261-14280 | Msa1358560:CDS | 45.0% | |
| GGGCTCCTTTAACAAGATCT+GGG | - | tig0016349:14262-14281 | Msa1358560:CDS | 45.0% | |
| ! | CAAGATCTGGGATTGGACTA+TGG | - | tig0016349:14274-14293 | Msa1358560:CDS | 45.0% |
| AATTCTCATTCAGCCGTTGC+AGG | - | tig0016349:14307-14326 | Msa1358560:CDS | 45.0% | |
| CCAAGTAATGATGTGTGCTG+TGG | + | tig0016349:14335-14354 | Msa1358560:intergenic | 45.0% | |
| CCACAGCACACATCATTACT+TGG | - | tig0016349:14332-14351 | Msa1358560:CDS | 45.0% | |
| GGACGGCAAGAACAGATGTT+AGG | - | tig0016349:14189-14208 | Msa1358560:CDS | 50.0% | |
| ! | ATACGTTGGGGATTGATGGC+AGG | - | tig0016349:14241-14260 | Msa1358560:CDS | 50.0% |
| ! | TACGTTGGGGATTGATGGCA+GGG | - | tig0016349:14242-14261 | Msa1358560:CDS | 50.0% |
| ! | CGTTGGGAGGTGTACAACCA+TGG | - | tig0016349:14217-14236 | Msa1358560:CDS | 55.0% |
| GTGTGCTGTGGTTCCTGCAA+CGG | + | tig0016349:14323-14342 | Msa1358560:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0016349 | gene | 14180 | 14398 | 14180 | ID=Msa1358560;Name=Msa1358560 |
| tig0016349 | mRNA | 14180 | 14398 | 14180 | ID=Msa1358560-mRNA-1;Parent=Msa1358560;Name=Msa1358560-mRNA-1;_AED=0.48;_eAED=0.58;_QI=0|-1|0|1|-1|1|1|0|72 |
| tig0016349 | exon | 14180 | 14398 | 14180 | ID=Msa1358560-mRNA-1:exon:10913;Parent=Msa1358560-mRNA-1 |
| tig0016349 | CDS | 14180 | 14398 | 14180 | ID=Msa1358560-mRNA-1:cds;Parent=Msa1358560-mRNA-1 |
| Gene Sequence |
| Protein sequence |
